Provided herein are methods for enrichment from a library of nucleic acid sequences of interest using primers against identification sequences. The nucleic acid sequences of interest can be subsequently cloned, and the protein products can be analyzed.

Provided herein are methods for enrichment from a library of nucleic acid sequences of interest using primers against identification sequences. The nucleic acid sequences of interest can be subsequently cloned, and the protein products can be analyzed.

A method of RNA synthesis via fluid flow apparatus. The method may involve introducing into a first fluid flow module, via a plurality of inlet ports, a plurality of reactants comprising: at least one nucleoside triphosphate (NTP), a reaction buffer, and DNA, a DNA based compound or a DNA based mixture; allowing at least some of the reactants to react within a reaction channel or well within the first module of the flow system; retaining or recirculating the DNA at the first reactor module and allowing reaction products of the reactants to flow into a first fluidic filtration module; and filtering the reaction products within the first filtration module.

A method of RNA synthesis via fluid flow apparatus. The method may involve introducing into a first fluid flow module, via a plurality of inlet ports, a plurality of reactants comprising: at least one nucleoside triphosphate (NTP), a reaction buffer, and DNA, a DNA based compound or a DNA based mixture; allowing at least some of the reactants to react within a reaction channel or well within the first module of the flow system; retaining or recirculating the DNA at the first reactor module and allowing reaction products of the reactants to flow into a first fluidic filtration module; and filtering the reaction products within the first filtration module.

The present invention provides circularized RNA and methods of making, purifying, and using same.

The present invention provides circularized RNA and methods of making, purifying, and using same.

Methods for detecting the presence of a pathogen infection are described. In particular, this document provides a method of detecting target nucleic acids, such as pathogen-specific RNA, in a biological sample obtained from a subject, where the method comprises using one or more toehold switch sensors and an isothermal amplification step to detect the target nucleic acid. Methods specific for detecting and identify the presence of a virus such as Zika virus are also provided.

Methods for detecting the presence of a pathogen infection are described. In particular, this document provides a method of detecting target nucleic acids, such as pathogen-specific RNA, in a biological sample obtained from a subject, where the method comprises using one or more toehold switch sensors and an isothermal amplification step to detect the target nucleic acid. Methods specific for detecting and identify the presence of a virus such as Zika virus are also provided.

This invention relates to methods for producing nucleic acid encoded compounds and libraries of nucleic acid encoded compounds. A nascent compound that comprises a scaffold connected to a solid support by a linker is covalently attached to one or more chemical building blocks to form a chemical portion attached to the scaffold. Coding oligonucleotides encoding the one or more chemical building blocks are covalently attached to the nascent compound to form a coding nucleic acid portion attached to the scaffold. A cleaving group is attached to the chemical portion, nucleic acid portion, or scaffold of the compound. The linker is then reacted with the cleaving group, such that the linker is cleaved and the compound released from the solid support. Nucleic acid encoded compounds and libraries and methods for their production are provided.

This invention relates to methods for producing nucleic acid encoded compounds and libraries of nucleic acid encoded compounds. A nascent compound that comprises a scaffold connected to a solid support by a linker is covalently attached to one or more chemical building blocks to form a chemical portion attached to the scaffold. Coding oligonucleotides encoding the one or more chemical building blocks are covalently attached to the nascent compound to form a coding nucleic acid portion attached to the scaffold. A cleaving group is attached to the chemical portion, nucleic acid portion, or scaffold of the compound. The linker is then reacted with the cleaving group, such that the linker is cleaved and the compound released from the solid support. Nucleic acid encoded compounds and libraries and methods for their production are provided.