Provided is a method for evaluating the viral clearance capability of a virus removal medium, comprising the steps of: (a) adding a viral capsid-containing liquid to a solution to be purified; (b) contacting the virus removal medium with the solution to be purified that has been supplemented with the viral capsid-containing liquid to harvest a purified solution; and (c) quantifying a total viral capsid in the solution to be purified before the purification and a total viral capsid in the purified solution.

A composition for improving the detection performance of fluorescent quantitative PCR. The composition comprises bovine serum albumin, sorbitol, ammonium sulfate, formamide, tetramethylammonium chloride, and at least one of dithiothreitol and betaine. The present invention further relates to a qPCR reaction liquid containing the composition and a preparation method therefor. The composition can improve the sensitivity, specificity, and interference resistance of real-time fluorescent quantitative PCR.

A composition for improving the detection performance of fluorescent quantitative PCR. The composition comprises bovine serum albumin, sorbitol, ammonium sulfate, formamide, tetramethylammonium chloride, and at least one of dithiothreitol and betaine. The present invention further relates to a qPCR reaction liquid containing the composition and a preparation method therefor. The composition can improve the sensitivity, specificity, and interference resistance of real-time fluorescent quantitative PCR.

Methods for use in multiplex amplification and or detection of Trichomonas vaginalis. The multiphase amplification provides fast, quantitative, sensitive detection with lower variability at low analyte concentrations. Described are detection probes, capture probes, amplification oligonucleotides, nucleic acid compositions, probe mixes, methods, and kits useful for amplifying and determining the presence of Trichomonas vaginalis in a test sample.

Methods for use in multiplex amplification and or detection of Trichomonas vaginalis. The multiphase amplification provides fast, quantitative, sensitive detection with lower variability at low analyte concentrations. Described are detection probes, capture probes, amplification oligonucleotides, nucleic acid compositions, probe mixes, methods, and kits useful for amplifying and determining the presence of Trichomonas vaginalis in a test sample.

Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

A method is provided comprising the following steps: (a) treating a nucleic acid with bisulfite to convert non-methylated cytosines in the nucleic acid into uracils while leaving methylated cytosines unchanged to form a treated nucleic acid strand that is part of two joined nucleic acid strands; (b) ligating a first adapter to a 3′ end of the treated nucleic acid strand, the first adapter having a first protruding random sequence that least 3 bases long and that acts as a splint for the two joined nucleic acid strands; (c) ligating a second adapter to a 5′ end of the once adapter ligated nucleic acid strand, the second adapter having a second protruding random sequence at least 3 bases long and that acts as a splint for the two joined nucleic acid strands; and (d) performing PCR amplification on the twice ligated nucleic acid strand.

A method is provided comprising the following steps: (a) treating a nucleic acid with bisulfite to convert non-methylated cytosines in the nucleic acid into uracils while leaving methylated cytosines unchanged to form a treated nucleic acid strand that is part of two joined nucleic acid strands; (b) ligating a first adapter to a 3′ end of the treated nucleic acid strand, the first adapter having a first protruding random sequence that least 3 bases long and that acts as a splint for the two joined nucleic acid strands; (c) ligating a second adapter to a 5′ end of the once adapter ligated nucleic acid strand, the second adapter having a second protruding random sequence at least 3 bases long and that acts as a splint for the two joined nucleic acid strands; and (d) performing PCR amplification on the twice ligated nucleic acid strand.

A microfluidic device for amplifying a nucleic acid includes a cartridge and a control part. The cartridge includes a tank part and a plurality of first chambers. The control part is configured to control execution of a thermal cycle, count a number of repetitions of the thermal cycle for each of the first chambers and store a count value, acquire a fluorescence intensity of each of the first chambers for each thermal cycle, and reset the count value of a defective chamber of which the fluorescence intensity is not within a predetermined range, discharge the solution from the defective chamber, and fill the defective chamber with a new solution from the tank part.