The present invention provides method and kits for amplification based detection of target nucleic acids and pathogens using crude biological samples without prior target purification.

The present invention provides method and kits for amplification based detection of target nucleic acids and pathogens using crude biological samples without prior target purification.

The present invention provides method and kits for amplification based detection of target nucleic acids and pathogens using crude biological samples without prior target purification.

The present disclosure provides a method and system for the PCR amplification of a target sequence which suppresses non-specific amplification products. The disclosure concerns the use of a primer pair optimized to amplify a nucleic acid of a contaminant in the background of genomic DNA of a first organism. When DNA from a second organism suspected for comprising the contaminant is subjected to the same PCR-based amplification reaction, detection sensitivity and specificity of the contaminant is enhanced when an amount of genomic DNA of the first organism is present in the amplification reaction.

The present invention is directed to compositions, methods and kits useful for the synthesis of nucleic acid molecules. More specifically, compositions, methods and kits are provided for the amplification of nucleic acid molecules in a one-step RT-PCR procedure comprising one or more agents used to increase tolerance to PCR inhibitors.

The present invention is directed to compositions, methods and kits useful for the synthesis of nucleic acid molecules. More specifically, compositions, methods and kits are provided for the amplification of nucleic acid molecules in a one-step RT-PCR procedure comprising one or more agents used to increase tolerance to PCR inhibitors.

The present disclosure provides a method and system for the PCR amplification of a target sequence which suppresses non-specific amplification products. The disclosure concerns the use of a primer pair optimized to amplify a nucleic acid of a contaminant in the background of genomic DNA of a first organism. When DNA from a second organism suspected for comprising the contaminant is subjected to the same PCR-based amplification reaction, detection sensitivity and specificity of the contaminant is enhanced when an amount of genomic DNA of the first organism is present in the amplification reaction.

Disclosed herein include systems, methods, compositions, and kits for performing intracellular AbSeq assays. There are provided, in some embodiments, methods for measuring intracellular target expression. The method can comprise fixing and permeabilizing a plurality of cells before contacting with a plurality of intracellular target-binding reagents capable of specifically binding to an intracellular target. Intracellular target-binding reagents can comprise an intracellular target-binding reagent specific oligonucleotide comprising a unique intracellular target identifier for the intracellular target-binding reagent specific oligonucleotide. The method can further comprise removing the permeabilizing agent and reversing the fixation.

Disclosed herein include systems, methods, compositions, and kits for performing intracellular AbSeq assays. There are provided, in some embodiments, methods for measuring intracellular target expression. The method can comprise fixing and permeabilizing a plurality of cells before contacting with a plurality of intracellular target-binding reagents capable of specifically binding to an intracellular target. Intracellular target-binding reagents can comprise an intracellular target-binding reagent specific oligonucleotide comprising a unique intracellular target identifier for the intracellular target-binding reagent specific oligonucleotide. The method can further comprise removing the permeabilizing agent and reversing the fixation.

Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of DNA polymerases, such as Taq DNA polymerase.