The present invention pertains to the field of pulp or paper making. More specifically the present invention relates to a method of preventing a build-up of slime or removing slime from a surface contacted with water from a pulp or paper making process. The present invention can control slime in an efficient and environmentally friendly way.

A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.

A method for the enzymatic production of D-fructose from D-glucose in a one-pot synthesis, wherein D-glucose is oxidized enzymatically to D-glucosone and D-glucosone is reduced enzymatically to D-fructose and the use of the D-fructose produced in this way for the production of furan derivatives.

A molecular manufacturing process includes contacting a platform molecule with (i) a biocatalyst and (ii) a chemical catalyst under conditions suitable to produce a value-added chemical.

Systems comprising a first polynucleotide comprising a regulatory element operatively linked to an open reading frame encoding an invertase enzyme and a second polynucleotide comprising a repressible regulatory element operatively linked to an open reading frame encoding a glucose oxidase enzyme wherein the repressible element inhibits transcription in response to hydrogen peroxide are provided. Cells and compositions comprising a system of the invention, as well as methods using the system of the invention, are also provided, as is artificial honey.

A method of synthesizing(S)-3-hydroxy--butyrolactone (HBL), the method including producing a trione using an enzymatic conversion of glucosone. The enzymatic conversion may include: mutating a domain of Aldos-2-ulose dehydratase (AUDH) to obtain a mutant AUDH; dehydrating glucosone using the mutant AUDH to obtain an intermediate, basidiopyrone P (BPP) (4,5-dihydroxy-2-(hydroxymethyl)-2H-pyran-5-one); and forming the trione from the BPP. The method may further include catalyzing the trione using a base to form an ester of 3,4-dihydroxybutyrate; and converting the ester of 3,4-dihydroxybutyrate into the HBL by adding an acid to the ester of 3,4-dihydroxybutyrate.