The present invention refers to a chimeric enzyme constructed for the production of terminal alkenes from fatty acids, so that the cofactor necessary for the reaction is produced in situ from industrial waste, favoring the circular economy. Furthermore, the present invention deals with the process of producing terminal alkenes using said chimeric enzyme. This invention belongs to the field of industrial biotechnology and finds application in the biofuels and renewable chemicals industry.

An alditol oxidase and application thereof. The method uses D-glucose as a substrate to dock the alditol oxidase derived from Streptomyces coelicolor A3, and selects amino acid residues around an active center for saturation mutagenesis, and screens for alditol oxidase with D-glucose oxidizing activity by plate color development. An amino acid sequence of the alditol oxidase is shown in SEQ ID NO: 2 or SEQ ID NO: 4. The alditol oxidase has the activity of converting D-glucose to D-gluconic acid and D-glyceraldehyde to D-glyceric acid. By using the alditol oxidase, the conversion of D-glucose to pyruvic acid is realized by only three enzymes for the first time and does not depend on any coenzyme.