The present disclosure disclosed is method for improving the transparency of starch liquefaction, and belongs to the field of biologically modified starch. The method changes the molecular structure of the starch liquefaction product by adding a 1,4-α-glucan branching enzyme from Rhodothermus obamensis, so the molecular structure has a smaller branched chain and a higher branching degree, thereby achieving the purpose of improving transparency and stability. The method comprises the steps of dissolving a starch liquefaction product in water according to a certain concentration to prepare an aqueous solution of the starch liquefaction product, and adding a 1,4-α-glucan branching enzyme to react at a certain temperature for a period of time, so as to improve the transparency of the starch liquefaction product during storage. The method provides a new idea for improving the stability of starch liquefaction products, and has great potential and application prospects.

Disclosed herein are methods for preparing modified starches, and uses thereof. A method for preparing a modified starch includes the steps of: modifying a gelatinized starch suspension with β-amylase; after inactivating the β-amylase, further modifying with a branching enzyme, after inactivating the branching enzyme, further modifying with pullulanase, after inactivating the pullulanase, precipitating a resulting enzymatic hydrolysate with an alcohol to obtain precipitates; and drying the precipitates to obtain the modified starch. The methods disclosed herein substantially increase the number of linear chains with a degree of polymerization (DP) 6-11 in the starch chains and, thus, significantly increase the content of resistant starch (RS) in the modified starch—thereby facilitating the use in foods and medicaments.

Disclosed is an enzyme composition for regulating sugar metabolism which can regulate the absorption of glucose into the body by converting the carbohydrates in food to a form of sugar that is not absorbed in the stomach and the like before being decomposed in the small intestine into glucose by the activity of various enzymes such as maltase, sucrase, or lactase and the like and absorbed, wherein the enzyme composition includes: one or more enzymes selected from the group consisting of glucoamylase, sucrase and lactase; glucose oxidase; and transglucosidase.

A series of independent human-induced non-transgenic mutations found at one or more of the SBEII genes of wheat; wheat plants having these mutations in one or more of their SBEII genes; and a method of creating and finding similar and/or additional mutations of SBEII by screening pooled and/or individual wheat plants. The seeds and flour from the wheat plants of the present invention exhibit an increase in amylose and resistant starch without having the inclusion of foreign nucleic acids in their genomes. Additionally, the wheat plants of the present invention exhibit altered SBEII activity without having the inclusion of foreign nucleic acids in their genomes.

Wheat grain (Triticum aestivum) comprising an embryo and starch, wherein the embryo comprises two identical alleles of an SBEIIa-A gene, two identical alleles of an SBEIIa-B gene and two identical alleles of an SBEIIa-D gene, wherein each of the SBEIIa genes gives rise to an amount of protein (w/w) or a protein having SBEIIa activity which is lower than the corresponding wild-type gene, and at least one of said genes comprises a point mutation, wherein the starch comprises amylose such that the grain has an amylose content of at least 50% (w/w) as a proportion of the extractable starch of the grain.

A series of independent human-induced non-transgenic mutations found at one or more of the SBEII genes of wheat; wheat plants having these mutations in one or more of their SBEII genes; and a method of creating and finding similar and/or additional mutations of SBEII by screening pooled and/or individual wheat plants. The seeds and flour from the wheat plants of the present invention exhibit an increase in amylose and resistant starch without having the inclusion of foreign nucleic acids in their genomes. Additionally, the wheat plants of the present invention exhibit altered SBEII activity without having the inclusion of foreign nucleic acids in their genomes.

The present disclosure disclosed is method for improving the transparency of starch liquefaction, and belongs to the field of biologically modified starch. The method changes the molecular structure of the starch liquefaction product by adding a 1,4--glucan branching enzyme from Rhodothermus obamensis, so the molecular structure has a smaller branched chain and a higher branching degree, thereby achieving the purpose of improving transparency and stability. The method comprises the steps of dissolving a starch liquefaction product in water according to a certain concentration to prepare an aqueous solution of the starch liquefaction product, and adding a 1,4--glucan branching enzyme to react at a certain temperature for a period of time, so as to improve the transparency of the starch liquefaction product during storage. The method provides a new idea for improving the stability of starch liquefaction products, and has great potential and application prospects.

Wheat grain (Triticum aestivum) comprising an embryo and starch, wherein the embryo comprises two identical alleles of an SBEIIa-A gene, two identical alleles of an SBEIIa-B gene and two identical alleles of an SBEIIa-D gene, wherein each of the SBEIIa genes gives rise to an amount of protein (w/w) or a protein having SBEIIa activity which is lower than the corresponding wild-type gene, and at least one of said genes comprises a point mutation, wherein the starch comprises amylose such that the grain has an amylose content of at least 50% (w/w) as a proportion of the extractable starch of the grain.

The invention relates to immobilized enzyme compositions for the preparation of a hexose. Hexoses include, for example, tagatose, psicose, fructose, allose, mannose, galactose, altrose, talose, sorbose, gulose, idose, and inositol. The invention also relates to an enzymatic process for preparing a hexose from a saccharide by contacting a starch derivative with an immobilized enzyme composition of the invention.

A series of independent human-induced non-transgenic mutations found at one or more of the SBEII genes of wheat; wheat plants having these mutations in one or more of their SBEII genes; and a method of creating and finding similar and/or additional mutations of SBEII by screening pooled and/or individual wheat plants. The seeds and flour from the wheat plants of the present invention exhibit an increase in amylose and resistant starch without having the inclusion of foreign nucleic acids in their genomes. Additionally, the wheat plants of the present invention exhibit altered SBEII activity without having the inclusion of foreign nucleic acids in their genomes.