A method for producing isoprene glycol, including: enzymatically generating 3-hydroxy-3-methylbutyryl-CoA (HMB-CoA) from acetyl-CoA (Ac-CoA); enzymatically generating 3-methyl-3-hydroxybutyrylaldehyde (3-HMBA) from HMB-CoA; and enzymatically converting 3-HMBA to isoprene glycol (ISPG). Wherein the enzymatically generating 3-HMBA from HMB-CoA includes: enzymatically reducing HMB-CoA to produce 3-HMB; or enzymatically hydrolyzing HMB-CoA to 3-hydroxy-3-methylbutyric acid (HMB) and then enzymatically reducing HMB to 3-HMBA. Also provided herein are an enzyme composition, a nucleic acid composition, and a transgenic microorganism for producing isoprene glycol.