One embodiment of the present invention provides a nonwoven fabric containing silk fibers in which an abs intensity ratio [abs (1650)/abs (1620)], which is a ratio of an intensity of a peak positioned in a vicinity of 1650 cm.sup.−1 [abs (1650)] in an infrared absorption spectrum to an intensity of a peak positioned in a vicinity of 1620 cm.sup.−1 [abs (1620)] in an infrared absorption spectrum, is larger than 0.65 and 1.90 or less, and a method for producing the nonwoven fabric containing silk fibers.

A method of producing a fibroin protein product includes providing a stock solution of fibroin protein, injecting a first injection solution including a chaotropic salt into the stock solution, injecting a second injection solution including a kosmotropic salt into the stock solution to provide an intermediate solution, and exposing the intermediate solution to a shear force. A method of preparing a stock solution of fibroin protein includes dissolving a silk fiber into a protic solvent to provide a solution including fibroin protein and adding a chaotropic salt to the solution including fibroin protein to provide the stock solution of fibroin protein. A 3D printer includes a first extruder, a closed duct assembly, at least one injection site, a filter assembly, a printer head assembly, and a printer body.

A method of producing a fibroin protein product includes providing a stock solution of fibroin protein, injecting a first injection solution including a chaotropic salt into the stock solution, injecting a second injection solution including a kosmotropic salt into the stock solution to provide an intermediate solution, and exposing the intermediate solution to a shear force. A method of preparing a stock solution of fibroin protein includes dissolving a silk fiber into a protic solvent to provide a solution including fibroin protein and adding a chaotropic salt to the solution including fibroin protein to provide the stock solution of fibroin protein. A 3D printer includes a first extruder, a closed duct assembly, at least one injection site, a filter assembly, a printer head assembly, and a printer body.

The disclosure relates to systems and methods for improving the manufacturing of silk solutions and powders containing silk fibroin obtained from silkworm cocoons. The solutions and powders can be used to improve the post-harvest preservation of perishables and to improve the performance of packaging, including biodegradable packaging.

The disclosure relates to systems and methods for improving the manufacturing of silk solutions and powders containing silk fibroin obtained from silkworm cocoons. The solutions and powders can be used to improve the post-harvest preservation of perishables and to improve the performance of packaging, including biodegradable packaging.

The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.

The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.

The disclosure relates to systems and methods for improving the manufacturing of silk solutions and powders containing silk fibroin obtained from silkworm cocoons. The solutions and powders can be used to improve the post-harvest preservation of perishables and to improve the performance of packaging, including biodegradable packaging.

The disclosure relates to systems and methods for improving the manufacturing of silk solutions and powders containing silk fibroin obtained from silkworm cocoons. The solutions and powders can be used to improve the post-harvest preservation of perishables and to improve the performance of packaging, including biodegradable packaging.

Composites based on a polymer and a mixture of proteins derived from a MaSp (major ampullate spidroin) protein are provides. Further, methods for preparation of same, and method of use of the composites are provided.