The present invention relates to determination of the microorganism content in material comprising cellulose within the pulp and paper industry. The material comprising cellulose is enzymatically pretreated and microorganisms are determined using PCR based technology.

The invention relates to xylanases and to polynucleotides encoding the xylanases. In addition, methods of designing new xylanases and methods of use thereof are also provided. The xylanases have increased activity and stability at increased pH and temperature.

Disclosed herein are cellulase variants, or active fragments thereof, and polynucleotides encoding same, wherein the cellulase variants, or active fragments thereof, have endoglucanase activity. Also disclosed herein are compositions comprising said cellulase variants, or active fragments thereof, vectors and/or host cells comprising the polynucleotides encoding said cellulase variants, or active fragments thereof; and methods for making and/or using said cellulase variants, or active fragments thereof and/or compositions containing same; wherein said cellulase variants, or active fragments thereof, have endoglucanase activity.

The invention is directed to novel variant endoglucanases and their use thereof.

Systems and methods as disclosed herein automatically provide real-time dosing corrections for an industrial process wherein enzyme blends are applied to natural fibers for pulp/ paper production. An initial enzyme blend (e.g., enzymes and supporting formulation components, as relevant) and respective dose rates are selected to be applied based on expected fiber surface substrate characterization, expected fiber quality characterization, the physical conditions of the system being treated, respective characteristics of the initially selected enzyme blend components, etc. Upon application of the initial enzyme blend, online sensors provide real-time feedback data corresponding to measured actual values for the fiber surface substrate characterization and fiber quality characterization. A replacement enzyme blend (enzymes and supporting formulation components) and respective dose rates thereof is dynamically selected based on the feedback data. The enzyme dosing stage can be optimized responsive to product changes and/or variations in fiber sources/blend and/or physical conditions, substantially in real time.

The present invention relates to GH61 polypeptide variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

A solution stable enzyme composition comprising an enzyme component, a stabilizer component, an optional antimicrobial preservative and water is disclosed, as well as its use in manufacturing of pulp and paper.

A method of making an absorbent structure including forming a stock mixture of fibers, a cationic wet strength resin, an anionic polyacrylamide and a cellulase enzyme, and at least partially drying the stock mixture to form a web.

The present disclosure provides methods of producing plants with preferred levels of cell wall biosynthesis; and uses of such plants. The inventors have identified that the GFR9, CCoAOMT and MYB41 genes are major regulators of the cell wall biosynthesis pathway. Plants with modulated cell wall biosynthesis, based on modulation of the expression or activity of the GFR9, CCoAOMT and MYB41 genes, have divergent uses including pulp and paper production, and bioproduct production.

The present invention relates to an enhanced process for the production of cellulose pulps with increased quality and applicability of said pulps, especially their physical resistance properties and degree of resistance to drainage, through an enzymatic treatment step comprised in the production process of said cellulose pulp, concomitantly with the polymer dosage based on carbohydrates.