An example system for inspecting a surface includes a laser, an optical system, a gated camera, and a control system. The laser is configured to emit pulses of light, with respective wavelengths of the pulses of light varying over time. The optical system includes at least one optical element, and is configured to direct light emitted by the laser to points along a scan line one point at a time. The gated camera is configured to record a fluorescent response of the surface from light having each wavelength of a plurality of wavelengths at each point along the scan line. The control system is configured to control the gated camera such that an aperture of the gated camera is open during fluorescence of the surface but closed during exposure of the surface to light emitted by the laser.

Optical imaging or spectroscopy described can use laminar optical tomography (LOT), diffuse correlation spectroscopy (DCS), or the like. An incident beam is scanned across a target. An orthogonal or oblique optical response can be obtained, such as concurrently at different distances from the incident beam. The optical response from multiple incident wavelengths can be concurrently obtained by dispersing the response wavelengths in a direction orthogonal to the response distances from the incident beam. Temporal correlation can be measured, from which flow and other parameters can be computed. An optical conduit can enable endoscopic or laparoscopic imaging or spectroscopy of internal target locations. An articulating arm can communicate the light for performing the LOT, DCS, or the like. The imaging can find use for skin cancer diagnosis, such as distinguishing lentigo maligna (LM) from lentigo maligna melanoma (LMM).

Disclosed are an optical spectroscopy system using a matched filter-based broadband signal receiver for stable data extraction, and a method for controlling the optical spectroscopy system. The optical spectroscopy system may comprise: a light transmission unit for irradiating light on a particular region of a subject by means of a plurality of light sources, wherein the light irradiated from the plurality of light sources is code-modulated by means of the Walsh codes and then irradiated; and a light receiving unit for detecting emergent light which has passed through the particular region, wherein the light source is identified by demodulating the light by means of the Walsh codes.

Provided herein are methods for classifying or characterizing a biological sample in vivo or ex vivo in real-time using time-resolved spectroscopy and/or electrical stimulation. A biological sample may produce a responsive fluorescence signal when irradiated by a light excitation signal or pulse at a predetermined wavelength. The responsive fluorescence signal may be recorded. The intensity of the excitation wavelength may be recorded and used to normalize the recorded responsive fluorescence signal. The biological sample may produce a responsive electrical signal in response to electrical stimulation. Raw fluorescence decay data may be generated from the responsive fluorescence signal and pre-processed. The pre-processed raw fluorescence decay data may be de-convolved to remove an instrument response function therefrom and generate true fluorescence decay data. The biological sample may be characterized in response to the responsive fluorescence signal, the responsive electrical signal, the normalized responsive fluorescence signal, and/or the true fluorescence decay data.

Provided herein are devices, systems, and methods for characterizing a biological sample in vivo or ex vivo in real-time using time-resolved spectroscopy. A light source generates a light pulse or continuous light wave and excites the biological sample, inducing a responsive fluorescent signal. A demultiplexer splits the signal into spectral bands and a time delay is applied to the spectral bands so as to capture data with a detector from multiple spectral bands from a single excitation pulse. The biological sample is characterized by analyzing the fluorescence intensity magnitude and/or decay of the spectral bands. The sample may comprise one or more exogenous or endogenous fluorophore. The device may be a two-piece probe with a detachable, disposable distal end. The systems may combine fluorescence spectroscopy with other optical spectroscopy or imaging modalities. The light pulse may be focused at a single focal point or scanned or patterned across an area.

A system for providing optical measurements and detection in optical spectrum analyzers (OSAs) with high dynamic range and high speed is disclosed. The system may include a slit to allow inward passage of an optical beam. The system may also include an optical portion to receive the optical beam. In some examples, the optical portion may include at least one optical splitter to split the optical beam into at least two optical paths. The system may also include an electrical portion to receive the optical beams split into the at least two optical paths. In some examples, the electrical portion may include at least one photodetector to receive each of the split optical beam. The electrical portion may also include at least one amplifier communicatively coupled to each of the at least one photodetector to amplify the split optical beam. The electrical portion may further include at least one analog-to-digital converter (ADC) communicatively coupled to each of the at least one amplifier to convert the split optical beams into digital signals.

The invention provides systems for characterizing a biological sample by analyzing emission of fluorescent light from the biological sample upon excitation and methods for using the same. The system includes a laser source, collection fibers, a demultiplexer and an optical delay device. All references cited herein are incorporated by reference in their entirety as though fully set forth. Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of-ordinary skill in the art in which this invention belongs.

Disclosed are a time division spread spectrum code-based optical spectroscopy system capable of controlling irradiation power and a method for controlling the optical spectroscopy system. The optical spectroscopy system may comprise: a light transmission unit for irradiating light to a particular region of a subject by means of a light source, wherein the light is irradiated so that the overall energy is consistently maintained by reducing the light irradiation time and increasing the strength of the light; and a light receiving unit for collecting emergent light which has passed through the particular region.

A processing apparatus, comprises: a first acquirer configured to acquire a first specific information distribution of an object based on acoustic waves propagating from the object onto which light is irradiated; a second acquirer configured to acquire a characteristic value of the first specific information distribution of the object; a third acquirer configured to acquire information indicating a correspondence between an optical coefficient and the characteristic value of the first specific information distribution; and a fourth acquirer configured to acquire the optical coefficient of the object using the characteristic value of the first specific information distribution of the object and the information indicating the correspondence.

An optical manufacturing process sensing and status indication system is taught that is able to utilize optical emissions from a manufacturing process to infer the state of the process. In one case, it is able to use these optical emissions to distinguish thermal phenomena on two timescales and to perform feature extraction and classification so that nominal process conditions may be uniquely distinguished from off-nominal process conditions at a given instant in time or over a sequential series of instants in time occurring over the duration of the manufacturing process. In other case, it is able to utilize these optical emissions to derive corresponding spectra and identify features within those spectra so that nominal process conditions may be uniquely distinguished from off-nominal process conditions at a given instant in time or over a sequential series of instants in time occurring over the duration of the manufacturing process.