The present invention relates to a diagnostic method for determining lung disease. The method comprises obtaining a plurality of spectra produced by spectroscopic interrogations of a plurality of cells. The method comprises determining a feature of interest from each spectrum of the plurality of spectra. The method comprises determining a distribution of the features of interest. The method comprises diagnosing a lung disease in dependence on the distribution of features of interest.

Devices, systems, and associated methods for detecting drops dispensed by a dropper are provided. For example, a drop detection device may include a light source and a light detector configured to be coupled to a drop dispenser such that the light source and light detector are disposed proximal of a distal dispensing tip of the drop dispenser. The light detector may be configured to receive a reflected portion of a beam of light from the light source, which is reflected by a drop dispensed through the dispensing tip of the drop dispenser. In some embodiments, a processing circuit is configured to analyze a signal provided by the light detector to detect the dispensed drop.

High-throughput methods and systems for using morphological and/or autofluorescence signatures of cells to characterize unknown cell/tissue types within a forensic sample are provided. Machine learning algorithms are used to correlate morphological and/or autofluorescence signatures to characteristics such as cell type.

A device for detecting (D) at least one predetermined particle (P) includes an interferometric element (EI) arranged so as to be illuminated by an incident radiation (L.sub.in) and comprising at least one so-called thin layer (CM) disposed on top of a so-called substrate layer (Sub), the particle being attached to a surface (Sm) of the thin layer, the interferometric element (EI) forming a Fabry-Pérot cavity with or without attached particle P; a matrix sensor (Det) adapted to detect an image comprising a first portion (P.sub.1) deriving from the detection of the incident radiation transmitted (L.sub.TBG) by the interferometric element alone and a second portion (P.sub.2) deriving from the detection of the incident radiation transmitted (L.sub.TP) by the interferometric element and any particle (O, P) attached to a surface (Sm) of the thin layer; a processor (UT) linked to the sensor and configured: to calculate, as a function of wavelengths of the incident radiation λ.sub.i i∈[1,m], the variation of intensity of at least one first pixel of the first portion, called first variation (F.sub.BG) and of at least one second pixel of the second portion, called second variation (F.sub.P), to determine a trend, as a function of the wavelengths of the incident radiation λ.sub.i i∈[1,m], of a phase shift ϕ.sub.i between the first variation and the second variation; to detect the attached particle when the phase shift ϕ.sub.i is not constant as a function of the wavelengths of the incident radiation λ.sub.i i∈[1,m].

A method for electromagnetic imaging of containers receives uncalibrated first data corresponding to signals of a first plurality of different frequencies associated with an antenna array residing in a container having contents. The method estimates of a second data based on a computer model and simulation of signals of a second plurality of different frequencies associated with the antenna array, the second plurality of different frequencies including a subset of the first plurality of different frequencies. The method compares magnitudes, without corresponding phase comparisons, of the first and second data at each frequency of the second plurality of different frequencies. The method updates the second data based on the comparing. The method provides information about the contents within the container based on the updated second data.

A first imaging unit obtains an image of at least one of a jet flow, droplets or satellite drops. Based on a feature value of the at least one of the jet flow, the droplets or the satellite drops in the image, a controller controls a timing of starting to supply charges from a charge supply unit to a final jet flow droplet in one period of vibrations of a vibration element or an amplitude of a drive voltage applied to the vibration element so as to cause variation of a side stream to fall within a reference range.

Provided herein are embodiments of methods and systems for screening cellular, subcellular, and multicellular structures. In one embodiment, a method for screening is provided comprising the steps of introducing a plurality of cellular, subcellular, or multicellular structures, or a combination thereof, to an imaging system, wherein one or more structures of the plurality comprise one or more taggable markers; imaging the plurality of structures using the imaging system; identifying one or more target structures among the plurality of structures based on one or more properties of the target structures; tagging the target structures to produce tagged target structures, wherein each target structure is selectively illuminated by an excitation light, thereby causing one or more taggable markers within the target structure to be phototransformed to produce one or more phototransformed taggable markers within the target structure; and isolating one or more tagged target structures from the plurality of structures.

Disclosed herein are methods and compositions for antimicrobial quantification and functional measurement. In one aspect, a method for quantifying antimicrobial comprises: obtaining a biological sample from a patient receiving an antimicrobial; incubating the biological sample with a reference microbial strain and a fluorophore for detecting cell lesion; measuring a first signal of fluorescent intensity in the incubated biological sample using flow cytometry; and comparing the first signal to a calibrating curve previously generated for the antimicrobial, thereby quantifying the antimicrobial present in the biological sample.

Disclosed is a multi-dimensional optical tweezers calibration device based on electric field quantity calibration and a method thereof. The polarization-dependent characteristics of a tightly focused optical trap are utilized to realize triaxial electric field force calibration of particles through a one-dimensional electric field quantity calibration device. The method of the present application enables a particle electric field force calibration system to be compatible with particle delivery and particle detection systems; the device is simplified and calibration complexity is reduced.

A system for orienting particles in a microfluidic system includes one or more radiation pressure sources arranged to expose particles to radiation pressure to cause the particles to adopt a particular orientation in the fluid. A system for sorting particles in a microfluidic system includes a detection stage arranged to detect at least one difference or discriminate between particles in the fluid flow past the detection stage, and one or more radiation pressure sources past which the particles move sequentially and a controller arranged to switch radiation energy to cause a change in direction of movement of selected particles in the fluid flow to sort the particles. The particles may be biological particles such as spermatozoa. The radiation pressure may be optical pressure and may be from one or more waveguides which may extend across a channel of the microfluidic system.