An efficient manual method for preparing a cytology sample for staining. According to the method, a biological sample is provided, wherein the biological sample is a liquid-based cytology sample. A slide is placed in a holder adapted for receiving the slide. The slide is pre-coated with a composition that will cause cells to adhere to the slide. A settling chamber is placed over said slide and the settling chamber is locked onto the holder. The settling chamber has openings in both proximate and distal ends. The opening in the distal end of the settling chamber is positioned over the slide such that the slide is interposed between the holder and the settling chamber and the slide is in fluid communication with the settling chamber. A density reagent is then dispensed into the settling chamber. The liquid-based cytology sample is then dispensed into the settling chamber over the reagent. The assembly formed by the holder, slide and settling chamber (and its liquid contents) is placed in a centrifuge. The assembly is centrifuged for less than 5 minutes at a rotation force of less than 500 g. The density reagent and the liquid thereover are decanted from the settling chamber. The settling chamber is removed from over the slide. The slide is removed from the holder for staining.

The present invention addresses the problem of providing a centrifugal smearing sample container holder having a structure which enables improvement of operability and easy assembly. A centrifugal smearing sample container holder (1) is provided with: a base part (2) including a bottom surface section (2a) that accommodates a sample container, a packing, and slide glass by being stacked, side surface sections (2b), (2c) formed on both sides of the bottom surface section (2a), locking sections (2e), (2f) respectively formed in the side surface sections (2b), (2c), a back surface section (2d) formed on a back side of the bottom surface section (2a), and a curl section (2g) formed in the back surface section (2d); and a holding part (3) including a handle section (3a) disposed on a front side of the bottom surface section (2a) along a short direction of the base part (2), arm sections (3e), (3f).

Exemplary embodiments of the inventive concept are directed to devices for facilitating the creation of pathology cell blocks and to methods for creating such cell blocks using said devices. Various cell block preparation devices are described, which cell blocks may be comprised of various media and may be provided with one or multiple wells for receiving and retaining tissue/cell specimens, such as cytology specimens. The tissue/cell specimen laden cell block preparation devices are treated with fixative to produce cell block specimens that are highly amenable to sectioning and subsequent analysis according to known methods. A marker may be integrated into any of the cell block preparation devices to guide a technologist during sectioning and subsequent application of cell block sections to slides for proper orientation of the cell block sections.

A quartz crystal microbalance coated with functionalized nanoparticles used to detect molecule-nanoparticle interactions to assist with characterization of difficult to predict molecule-nanoparticle interactions for novel ligand chemistries and, particularly, mixed ligand nanoparticles exhibiting different ligand morphologies, in order to quantify nanoparticle-molecule interactions independently from more complex solvation requirements.

A spectroscopy and artificial intelligence-interaction serum analysis method includes: collecting bulk SERS spectral data of clinical serum samples, performing dimension reduction on the spectral data by using a covariance matrix to obtain spectral different peak positions of cancer patients and normal individuals, and performing spectral data processing and algorithm identification by using an svm model of an artificial intelligence algorithm to obtain a cancer identification rate. Compared with the conventional serum analysis method, the spectroscopy and artificial intelligence-interaction serum analysis method requires no antibody-antigen or other biological specificity modification processes, and the serum of cancer patients and normal individuals can be identified more cheaply, rapidly and accurately. Also the different peak positions in SERS spectra of a large amount of serum samples can be located, which provides an entirely novel detection and analysis method at a molecular bond energy level for the field of liquid biopsy of clinical cancers.

A centrifugal smearing device including a sealed rotating container that houses a chamber with a slide glass removably attached for holding a liquid sample to smear cells with a centrifugal force onto the slide glass, and a base housing the sealed rotating container and including rotational driving means for rotating the sealed rotating container, the sealed rotating container includes a main body capable of housing the chamber and a lid for closing an upper surface opening portion of the main body, a seal portion for sealing a space between the main body and the end face of the upper face opening portion is provided on an outer circumferential edge of the lid, and a scattering protection wall is formed at an inside of the seal portion so as to protrude downward to hide the seal portion from inside so that a liquid does not scatter onto the seal portion.

Provided is a lid stand that can accommodate a lid of a sealed rotating container of a centrifugal smearing device in a leaned state and so that liquid dripping can be prevented, without securing a wide space on a desk, and that can similarly lower a risk of touching a liquid specimen also for both right-handed and left-handed operators. A lid stand (1) of the present invention is a lid stand against which a lid (142) fitted in a sealed rotating container (134) of a centrifugal smearing device (130) and having a flange portion (151) protruding in a radial direction in a peripheral portion (142d) and a cylindrical scattering protective wall (160) protruding to a rear surface direction is placed by leaning, wherein: a center supporting member (10) having an upper supporting portion (12) supporting a center vicinity portion (142c) of the lid (142) on both one side and the other side is disposed at a center position; and a lateral side supporting member (20) having a lower supporting portion (22) supporting the peripheral portion (142d) of the lid (142) is disposed at positions on both sides being the one side and the other side with respect to the center supporting member (10).

The present invention relates to a process for preparing and analyzing a plurality of cell suspensions (5) comprising at least the following successive steps: (a) loading a plurality of bottles (4) onto a reception plate (6), each bottle (4) comprising a cell suspension (5) to be analyzed; (b) loading a plurality of analysis containers (32, 34, 36) onto the reception plate (6); and (c) taking a sample of a cell suspension (5) from a bottle (4) and depositing this sample in an analysis container (34, 36); wherein step (c) is repeated for each bottle (4) to be analyzed. Step (c) of taking a sample of a cell suspension from a bottle (4) and depositing this sample in an analysis container (34, 36) comprises at least one step of breaking up the cell clusters by virtue of pipetting distribution means (20). The invention also relates to an automated device for implementing this process.

A biological sample is provided, wherein the biological sample is a liquid-based cytology sample. A slide is placed in a holder. The slide is pre-coated with a composition that will cause cells to adhere to the slide. A settling chamber is placed over the slide and the settling chamber is locked onto the holder. The settling chamber has openings in both proximate and distal ends. The opening in the distal end of the settling chamber is positioned over the slide. A density reagent is then dispensed into the settling chamber. The liquid-based cytology sample is then dispensed into the settling chamber over the reagent. The assembly formed by the holder, slide and settling chamber (and its liquid contents) is placed in a centrifuge. The density reagent and the liquid thereover are decanted from the settling chamber. The settling chamber is removed from over the slide.

A biological sample is provided, wherein the biological sample is a liquid-based cytology sample. A slide is placed in a holder. The slide is pre coated with a composition that will cause cells to adhere to the slide. A settling chamber is placed over the slide and the settling chamber is locked onto the holder. The settling chamber has openings in both proximate and distal ends. The opening in the distal end of the settling chamber is positioned over the slide. A density reagent is then dispensed into the settling chamber. The liquid-based cytology sample is then dispensed into the settling chamber over the reagent. The assembly formed by the holder, slide and settling chamber (and its liquid contents) is placed in a centrifuge. The density reagent and the liquid thereover are decanted from the settling chamber. The settling chamber is removed from over the slide.