Disclosed are a method for determining a platelet concentration of a blood sample, a hematology system and a storage medium. The method for determining a platelet concentration in a blood sample includes: forming a first suspension by mixing a first aliquot of the blood sample with a diluent; forming a second suspension by mixing a second aliquot of the blood sample with a lytic agent and a fluorescent dye to lyse red blood cells and stain white blood cells; measuring DC impedance signals of the first suspension passing through an aperture; measuring light scatter signals and fluorescent signals of the second suspension passing through an optical flow cell; analyzing DC impedance signals of the first suspension to obtain a first platelet distribution; analyzing light scatter signals and fluorescent signals of the second suspension to differentiate platelets from white blood cells and to obtain a second platelet distribution; and determining platelet data, such as the platelet concentration of the blood sample using the first and second platelet distributions.

The analysis method includes a step of measuring cytometry parameters for each biological cell contained in a biological sample; a step of determining, for each biological cell of the biological sample, a point in a N-dimensional space whose coordinates are defined depending on the cytometry parameters measured for the corresponding biological cell, where N is an integer greater than or equal to 3; a step of automatic clustering of the points into different cell clusters depending on the measured cytometry parameters, so as to define a sample cluster file; and a step of comparing the sample cluster files with reference cluster files, each of the reference cluster files being defined from cytometry parameters of a respective pathological or abnormal biological sample.

Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer.

An analyzing system includes an imaging cytometer having a first imaging section configured to perform an imaging of particles contained in an analyte sample to generate images, the imaging cytometer being configured to analyze the images generated by the first imaging section, and a blood cell analyzing apparatus configured to analyze particles contained in the analyte sample by at least one of an electrical resistance method and an optical analyzing method. One of the imaging cytometer and the blood cell analyzing apparatus has an analyzing section configured to produce a report of an analysis of the analyte sample based on particle information acquired by the imaging cytometer and particle information acquired by the blood cell analyzing apparatus.

For analyzing a sample containing particles of at least two categories, such as a sample containing blood cells, a particle counter subject to a detection limit is coupled with an analyzer capable of discerning particle number ratios, such as a visual analyzer, and a processor. A first category of particles can be present beyond detection range limits while a second category of particles is present within respective detection range limits. The concentration of the second category of particles is determined by the particle counter. A ratio of counts of the first category to the second category is determined on the analyzer. The concentration of particles in the first category is calculated on the processor based on the ratio and the count or concentration of particles in the second category.

The present disclosure relates to apparatus, systems, compositions, and methods for analyzing a sample containing particles. In some aspects the system comprises an analyzer which may be a visual analyzer. In one aspect, this disclosure relates to a particle imaging system comprising a flowcell through which a sample containing particles is caused to flow, and a high optical resolution imaging device which captures images for image analysis of samples. Other compositions, methods and features of this disclosure are disclosed herein.

A microfluidic device (10) for full blood count includes a first measurement channel (11) and a second measurement channel (12) separated from the first measurement channel (11). The microfluidic device (10) furthermore includes a first inlet (13) for providing a whole blood sample to the first and second measurement channel (11, 12), a second inlet (14) for providing a lysis agent for white blood cell count in to the first channel (11), a third inlet (15) for providing a quench solution to the first channel (11), and a fourth inlet (16) for providing a lysis agent for hemoglobin measurement to the second channel (12). A method for forming such a microfluidic device (10) and a method for performing a full blood count test using such a microfluidic device (10) are described.

A flow cytometry system including a measuring chamber, an injection device arranged to inject a flow of biological particles to be analyzed in the measuring chamber, an evacuation device arranged to evacuate outside of the cytometry system the flow of biological particles injected in the measuring chamber, a measuring set arranged to measure at least one optical property of the biological particles to be analyzed, the measuring set including an emission device arranged to emit a light beam in the direction of the measuring chamber and capable of crossing the flow of biological particles, and at least one collecting device arranged to collect light rays coming from the measuring chamber, where the flow cytometry system further includes a support on which the injection device, the evacuation device, the emission device and the at least one collecting device are mounted.

In the particle analyzing apparatus of the present invention, first, an inner space with a negative pressure having a predetermined volume is formed in the cylinder of a syringe device for sucking a sample liquid in the measuring chamber, then, the negative pressure is applied to the measuring chamber, the sample liquid is sucked, and measurement of particle is performed in the measuring flow path. The control device calculates a particle analysis value from the measurement signal obtained by the measurement. The particle analysis value is obtained by the sucking force of the negative pressure and the control device further corrects the particle analysis value based on a standard pressure predetermined for the inner space.

Disclosed are a method for determining a platelet concentration of a blood sample, a hematology system and a storage medium. The method for determining a platelet concentration in a blood sample includes: forming a first suspension by mixing a first aliquot of the blood sample with a diluent; forming a second suspension by mixing a second aliquot of the blood sample with a lytic agent and a fluorescent dye to lyse red blood cells and stain white blood cells; measuring DC impedance signals of the first suspension passing through an aperture; measuring light scatter signals and fluorescent signals of the second suspension passing through an optical flow cell; analyzing DC impedance signals of the first suspension to obtain a first platelet distribution; analyzing light scatter signals and fluorescent signals of the second suspension to differentiate platelets from white blood cells and to obtain a second platelet distribution; and determining platelet data, such as the platelet concentration of the blood sample using the first and second platelet distributions.