The present technology is to provide technology for appropriately visualizing a population of particles in particle analysis technology.

There is provided an information processing apparatus including an information processing unit that receives optical data obtained from particles, and calculates a parameter that specifies a display method of the optical data in a display range having at least one axis including a linear axis and a logarithmic axis on the basis of the received optical data, in which the parameter includes a first parameter that specifies a range of the linear axis and a second parameter that specifies a lower limit value of the display range, and the first parameter and the second parameter are calculated on the basis of different reference values.

An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.

A fractionated photoacoustic flow cytometry (PAFC) system and methods for the in vivo detection of target objects in biofluidic systems (e.g., blood, lymph, urine, or cerebrospinal fluid) of a living organism is described. The fractionated system includes a fractionated laser system, a fractionated optical system, a fractionated acoustic system, and combinations thereof. The fractionated laser system includes at least one laser or laser array for pulsing a target object within the circulatory vessel with fractionated focused laser beams. The fractionated optical system separates one or several laser beams into multiple beams in a spatial configuration on the skin above the circulatory vessel of the living organism. The fractionated acoustic system includes multiple focused ultrasound transducers for receiving photoacoustic signals emitted by the target object in response to the fractionated laser beams.

Disclosed herein include embodiments of a system, a device, and a method for sorting a plurality cells of a sample. A plurality of raw images comprising pixels of complex values in a frequency space can be generated from a plurality of channels of fluorescence intensity data of fluorescence emissions of fluorophores, the fluorescence emissions being elicited by fluorescence imaging using radiofrequency-multiplexed excitation in a temporal space. Spectral unmixing can be performed on the raw images prior to a sorting decision being made.

A cell sorting system that automatically generates a sorting strategy based on examples of target events provided by an operator. The target events can be selected using measurements ranging from traditional flow cytometry measurements to derived measurements that are computationally expensive to complex measurements such as images.

To reduce a data amount. An information processing system according to an embodiment includes an excitation light source (100) that irradiates a respective plurality of samples belonging to a sample group with excitation light, a measurement unit (142) that measures fluorescence generated by irradiation of the samples with the excitation light, and an information processing unit (2) that generates differential data based on a difference between similar fluorescence signals among fluorescence signals based on the fluorescence measured for the respective samples.

The invention relates to a formulation for total and differential counting of leukocytes for use in clinical analyses, in particular hemograms, to promote instantaneous differential staining of leukocytes in liquid medium, allowing greater practicality and agility in the total and differential counting of leukocytes. The practicality and agility are related to the use of a single dye for two parameters, eliminating the need for a smear. It results in a satisfactory effect for staining cells in suspension, staining the nucleus and cytoplasm of leukocytes in different shades, allowing their visualization under an optical microscope or in Point-of-Care image processing devices. It uses easily accessible raw materials, including cresyl acetate violet, ethyl alcohol, sodium and potassium chlorides, sodium hydroxide, acetic acid, triton X and deionized water, presenting a simple manufacturing process and low cost.

Some embodiments of the methods provided herein relate to sample analysis and particle characterization methods. Some such embodiments include receiving, from a particle analyzer, measurements for a first portion of particles associated with an experiment. Some embodiments also include generating a tree representing groups of related particles based at least in part on the measurements, wherein the tree includes at least three groups. Some embodiments also include generating a measure of relatedness between a first group and a second group of the tree based at least in part on the measurements. Some embodiments also include and configuring the particle analyzer to classify a subsequent particle associated with the experiment with the first group real-time, wherein the subsequent particle is not included in the first portion of particles. Some embodiments also include sorting the subsequent particle.

Provided is a light scattering cell classification technology which can classify cells into various types and at the same time classify cells with very high accuracy despite the rotation of the cells. A cell analysis apparatus using a plurality of lasers, according to an embodiment of the present invention, comprises: a plurality of laser generators which are installed around a movement path through which cells to be classified are moved, and which irradiate laser beams at one measurement point on the movement path at different angles; a plurality of photodetectors, installed around the one measurement point, which collect a second laser beam, which is a laser beam generated as the laser beams irradiated from the laser generators are incident on the cells and then scattered; and a cell analysis unit which classifies the cells to be classified according to the second laser beam collected by the photodetectors.

A computer-implemented method for data analysis comprises obtaining a plurality of first observations, each one of the plurality of first observations including one or more values of one or more first parameters, the plurality of first observations grouped into a plurality of groups; constructing a first histogram using the values of at least one of the one or more first parameters, included in the plurality of first observations; constructing, for each one of the plurality of groups, a second histogram having bins corresponding to bins of the first histogram, wherein each one of the bins of the second histogram includes a count of the first observations, among the first observations that belong to the one of the plurality of groups, having one or more values corresponding to the one of the bins for the at least one of the one or more first parameters; and outputting the second histograms.