The present invention relates to methods, monitors and systems, useful, for example, for advanced detection of sepsis in a subject.

The present invention pertains to novel analgesics useful for treating mechanical pain. The invention suggests the use of inhibitors of -tubulin acetylation for inhibition of neurological sensations that are mediated by sensory neurons. The perception of mechanical pain is can be modulated by altering the -tubulin acetylation, in context of the invention in particular by modulation of the expression and/or activity of the enzyme -tubulin acetyltransferase (Atat). The invention provides the medical application of -tubulin acetyltransferase inhibitors as analgesics and a screening method for the identification of compounds useful in the treatment of pain.

The present invention relates to methods, monitors and systems, useful, for example, for advanced detection of sepsis in a subject.

The present invention relates to methods, monitors and systems, useful, for example, for advanced detection of sepsis in a subject.

The present invention relates to methods, monitors and systems, useful, for example, for advanced detection of sepsis in a subject.

The invention relates to methods for quantitative multiplex analysis of complex protein samples from plants using mass spectroscopy. In some embodiments, the disclosure concerns methods for maintaining a transgenic plant variety, for example by analyzing generations of a transgenic plant variety for selective and sensitive quantitation of multiplexed transgenic proteins.

A method for determining activity of an acyl transferase enzyme, the method comprising: (i) preparing a reaction mixture comprising: (a) an acyl transferase enzyme, (b) a peptide substrate bound to a fluorophore, wherein the substrate is a cysteine-containing oligopeptide of 5-25 amino acids in length, (c) an acyl-CoA, and (d) a detergent comprising micelles, wherein the acyl transferase enzyme mediates acylation on a cysteine of said peptide substrate to result in association of the peptide with micelles of the detergent with resultant increase in fluorescence polarization; and (ii) measuring fluorescent signal of the reaction mixture; wherein an increase in fluorescence polarization of the reaction mixture compared to fluorescence polarization of a control reaction indicates acyl transferase activity of the acyl transferase enzyme. The above assay method may also be used for screening compounds for their ability to act as inhibitors of an acyl transferase enzyme.