The invention concerns a method for producing a chromatography analysis column, the resulting column, and a device comprising such a column. The method according to the invention comprises the following steps: (a) depositing on the flat surface of a substrate a first layer of particles which are intended to form the stationary phase; (b) depositing on the layer at least one second layer of compactly assembled particles; (c) impregnating the first and second layers with a light radiation-sensitive material, to form at least two compactly assembled particle layers impregnated with sensitive material; (d) insolating these layers in the regions corresponding to the desired internal shape of the chromatography analysis column, if the light radiation-sensitive material behaves like a positive resin, or outlining this internal shape if the light radiation-sensitive material behaves like a negative photosensitive resin; (e) eliminating either the regions insolated in step (d) if the light radiation-sensitive layer behaves like a positive photosensitive resin, or the regions not insolated in step (d) if the light radiation-sensitive material behaves like a negative photosensitive resin; and (f) covering and sealing the structure obtained in step (e) with a cover covered on the face facing the layers with at least one layer of compactly assembled particles which are identical to or different from those deposited on the substrate surface. The invention is used in particular in the field of chemical analysis.

The present invention relates to a chromatography column (100; 100′) comprising a tubular side wall (110) having an inner wall (112), an adaptor assembly (120) and a base assembly (125). An enclosed bed space (130) is defined between said adaptor assembly (120), said base assembly (125) and said inner wall (112) of said tubular side wall (110). The adaptor assembly (120) is axially movable inside said tubular side wall (110) in relation to said base assembly (125). The inner wall (112) comprises at least one groove (180; 180′; 180″; 180′″) which is positioned in a lower half of the tubular side wall (110) of the chromatography column (100), via which groove (180; 180′; 180″; 180′″) air and other fluid can pass the adaptor assembly (120) when the adaptor assembly is positioned in a priming position, in which priming position said adaptor assembly intersects with at least a part of the at least one groove (180; 180′; 180″; 180′″).

The present invention relates to a chromatography column (100; 100′) comprising a tubular side wall (110) having an inner wall (112), an adaptor assembly (120) and a base assembly (125). An enclosed bed space (130) is defined between said adaptor assembly (120), said base assembly (125) and said inner wall (112) of said tubular side wall (110). The adaptor assembly (120) is axially movable inside said tubular side wall (110) in relation to said base assembly (125). The inner wall (112) comprises at least one groove (180; 180′; 180″; 180′″) which is positioned in a lower half of the tubular side wall (110) of the chromatography column (100), via which groove (180; 180′; 180″; 180′″) air and other fluid can pass the adaptor assembly (120) when the adaptor assembly is positioned in a priming position, in which priming position said adaptor assembly intersects with at least a part of the at least one groove (180; 180′; 180″; 180′″).

The invention relates to a system and method for the stable storage of sensitive biological or chemical target substance, in a bound form on certain capture media. The method comprised providing a sample containing the target substance in a suitable buffer; combining the sample with a capture media to effect reversible binding of the target substance to the capture media; and storing the capture media with the target substance at between about −20 and 20° C.; and recovering the target substance from the capture media. The target substance recovered maintains the desired activity. Also provides are methods for reducing aggregates in the sensitive biological or chemical target substance.

The invention relates to a system and method for the stable storage of sensitive biological or chemical target substance, in a bound form on certain capture media. The method comprised providing a sample containing the target substance in a suitable buffer; combining the sample with a capture media to effect reversible binding of the target substance to the capture media; and storing the capture media with the target substance at between about −20 and 20° C.; and recovering the target substance from the capture media. The target substance recovered maintains the desired activity. Also provides are methods for reducing aggregates in the sensitive biological or chemical target substance.

Methods and systems for chromatography are disclosed that employ a flexible container configured to fit within a support structure and adapted to receive a filtration or absorptive medium. The flexible container can include at least one inlet, at least one outlet, and a separation barrier peripherally sealed within the container to separate the container into a resin containing portion and a drainage portion. The barrier can be configured to exclude the resin material from the drainage portion while allowing fluids to pass therethrough. The disposable chromatography system can further include one or more agitators disposed within the flexible container and adjustably configured to be raised or lowered in the flexible container. When the agitator is in the raised position, the resin packing material can operate in a settled, packed-bed configuration. Alternatively, the agitator in the lowered position permits the chromatography resin packing material to operate in a mixed, slurry configuration.

Methods and systems for chromatography are disclosed that employ a flexible container configured to fit within a support structure and adapted to receive a filtration or absorptive medium. The flexible container can include at least one inlet, at least one outlet, and a separation barrier peripherally sealed within the container to separate the container into a resin containing portion and a drainage portion. The barrier can be configured to exclude the resin material from the drainage portion while allowing fluids to pass therethrough. The disposable chromatography system can further include one or more agitators disposed within the flexible container and adjustably configured to be raised or lowered in the flexible container. When the agitator is in the raised position, the resin packing material can operate in a settled, packed-bed configuration. Alternatively, the agitator in the lowered position permits the chromatography resin packing material to operate in a mixed, slurry configuration.

Chromatography apparatus and methods are described, especially for expanded bed adsorption. A column tube has a process fluid input device at the bottom and a movable piston in the top. The piston is enclosed in the column by a cover plate. The piston body has an inflatable seal, and is connected by a frame to a contact ring which carries another inflatable member to contact the tube wall. Process fluid leaves the operating volume through an opening of the piston and flexible hose, through the enclosed space and out through the cover plate. The space above the piston can be pressurised to control piston movement. The contact ring maintains piston alignment. The inflatable seals are used to fix the piston in position, allow it to slide or allow washing. The piston outlet may include a vortex-inhibitor. Bed and piston levels may be monitored by ultrasound sensors.

Chromatography apparatus and methods are described, especially for expanded bed adsorption. A column tube has a process fluid input device at the bottom and a movable piston in the top. The piston is enclosed in the column by a cover plate. The piston body has an inflatable seal, and is connected by a frame to a contact ring which carries another inflatable member to contact the tube wall. Process fluid leaves the operating volume through an opening of the piston and flexible hose, through the enclosed space and out through the cover plate. The space above the piston can be pressurised to control piston movement. The contact ring maintains piston alignment. The inflatable seals are used to fix the piston in position, allow it to slide or allow washing. The piston outlet may include a vortex-inhibitor. Bed and piston levels may be monitored by ultrasound sensors.

A medical separation device, its production, and its use are provided, wherein the device has a hollow cylindrical housing sealed at its top and bottom sides, wherein the device has an outer wall, a fluid inlet, and a fluid outlet, and wherein the device has a filling connection for a separation medium arranged tangentially to, and inside of, the cylindrical housing outer wall for filling the device with a separation medium.