A method of processing of a biological sample containing multiple metabolites is described The method comprising the steps of pre-treating the biological sample with a metabolite extraction solvent to provide a pre-treated sample, separating a first aliquot of the pretreated sample by reverse phase liquid chromatography (RPLC) to provide a first eluent containing resolved hydrophobic metabolites, and separating a second aliquot of the pre-treated sample by hydrophilic interaction liquid interaction chromatography (HILIC) to provide a second eluent containing resolved hydrophilic metabolites. The first and second eluents are assayed using targeted tandem mass spectroscopy operated in multiple reaction monitoring mode. Each liquid chromatography step (LC) is directly hyphenated with the tandem mass spectrometry (MS/MS) into a single LC-MS/MS analysis. The extraction solvent typically comprises methanol, isopropanol and an acetate buffer.

Methods for determining the relative abundance of viral capsid components in a sample of viral particles are disclosed. In embodiments, methods for determining the relative abundance of empty capsids, partially-full capsids and full capsids (e.g., containing a heterologous nucleic acid molecule) of adeno-associated virus are disclosed.

An analysis method includes: performing liquid chromatography using a mobile phase including an adsorption prevention agent for preventing adsorption of a sample including a compound having a phosphate group to metal; and performing mass spectrometry on an eluate of the liquid chromatography. The adsorption prevention agent includes an oxalic acid or a salt of the oxalic acid.

Methods and system for identification of dimer species using online chromatography and electrospray ionization mass spectrometry are provided. Also provided are methods and system for quantitation of heterodimer species using immunoprecipitation and liquid chromatography-mass spectrometry.

This disclosure provides liquid chromatography tandem mass spectrometer (LC-MS/MS) methods and systems for detecting low levels of pesticides and mycotoxins in a test sample. In the disclosed methods and systems, oxalic acid is added to a mobile phase composition of a reverse phase chromatographic separation column. This addition improves the signal for certain pesticides and mycotoxins by a factor of from 1.5 to 9, improving their detection limits in a variety of test samples.

A data processing device comprises a processor unit adapted to process a plurality of initial data vectors provided by a chromatograph and/or a mass spectrometer, the processing being carried out in one, two or more processing steps producing items of processed data, and a storage unit adapted to save and retrieve initial data vectors and/or items of processed data, in particular processed data vectors or identified compounds, and/or items of additional data, in particular properties of the sample introduced in the mass spectrometer. Each item of processed data and/or additional data is connected to at least one initial data vector, and wherein the processor unit is adapted to group, select and/or modify initial data vectors and/or items of processed data according to one or more items of additional data.

Exemplary embodiments are directed to devices for separating a sample by chromatography, components of the devices, and methods for using the devices, and directed to devices and components for use with immobilized enzymatic reactors. A device includes a wall having a wetted surface exposed to a mobile phase including the sample during chromatographic separation. The wetted surface of the wall includes an alloy material including the following constituents: nickel, and cobalt and/or chromium where the alloy is limited in an amount of titanium to 1 wt %. A component includes a body having a wetted surface exposed to a mobile phase including the sample during chromatographic separation. The wetted surface of the body includes an alloy material including the following constituents: nickel, and cobalt and/or chromium where the alloy is limited in an amount of titanium to 1 wt %.

A kit or composition for in situ simultaneously derivatization of 14 phenol and carboxylic acid metabolites of benzene, toluene, ethylbenzene, and xylene (BTEX) in a urine sample is disclosed. The derivatization imparts a positive charge to phenol and carboxylic acid for subsequent LC-MS analysis. Limit of detection reached part-per-trillion levels for o-Cresol and part-per-billion levels for the remaining BTEX metabolites. BTEX metabolites can be detected in less than 35 mins according to one embodiment of the invention. Methods, kits and compositions disclosed herein can be used for in situ simultaneous derivatization of phenol and carboxylic acid in aqueous solution in general.

The present invention relates to the technical field of chemical analysis and quantitative detection, in particular to a quantitative detection method for snake venom thrombin-like enzyme (SVTLE) from Agkistrodon halys pallas. The quantitative detection method for the SVTLE includes the following steps of taking a reference substance of marker peptide for the SVTLE from Agkistrodon halys pallas with an amino acid sequence of LDSPVSNSAHIAPLSLPSSAPSVGSVCR, and preparing a series of reference solutions with different concentrations; adding the reference solutions in test solutions respectively for enzymolysis, and then taking a supernatant after enzymolysis as a series of solutions to be detected; and adding the solutions to be detected in a liquid chromatogram-mass spectrometer, and then selecting a qualitative ion pair and a quantitative ion pair to detect contents of marker peptide in the solutions to be detected.

An ion source assembly for use in a mass spectrometry system comprises a housing defining an ionization chamber disposed in fluid communication with a sampling orifice of a mass spectrometer system. The housing defines a first opening for coupling to a first electrospray probe to discharge a liquid sample at flow rates greater than a nanoflow range along a longitudinal axis that is substantially orthogonal to a central axis of the sampling orifice. An elongate auxiliary electrode assembly extends from the housing to an electrically conductive distal end disposed in the ionization chamber such that the electrically conductive distal end is disposed substantially on the central axis of the sampling orifice. The electrically conductive distal end may be coupled to a power supply to generate an electric field to improve the desolvation of the sample plume and the transport of ions ejected from the sample plume into the sampling orifice.