A magnification-variable optical system having a small size, a wide angle of view, and high optical performance, an optical apparatus including the magnification-variable optical system, and a method for manufacturing the magnification-variable optical system are provided. A magnification-variable optical system ZL used for an optical apparatus such as a camera 1 includes a first lens group G1 having a negative refractive power and including at least two lenses, and a rear group GR including at least one lens group disposed on an image side of the first lens group G1, and is configured so that a distance between lens groups adjacent to each other changes at magnification change and a condition expressed by predetermined condition expressions is satisfied.

A magnification-variable optical system having a small size, a wide angle of view, and high optical performance, an optical apparatus including the magnification-variable optical system, and a method for manufacturing the magnification-variable optical system are provided, the magnification-variable optical system ZL being used for an optical apparatus and includes a first lens group G1 having a negative refractive power, and a rear group GR including at least one lens group disposed on an image side of the first lens group G1, and is configured so that a distance between lens groups adjacent to each other changes at magnification change and a condition expressed by predetermined condition expressions is satisfied.

A magnification-variable optical system having a small size, a wide angle of view, and high optical performance, an optical apparatus including the magnification-variable optical system, and a method for manufacturing the magnification-variable optical system are provided, the magnification-variable optical system ZL being used for an optical apparatus and includes a first lens group G1 having a negative refractive power, and a rear group GR including at least one lens group disposed on an image side of the first lens group G1, and is configured so that a distance between lens groups adjacent to each other changes at magnification change and a condition expressed by predetermined condition expressions is satisfied.

The present disclosure discloses an optical imaging lens assembly including, sequentially from an object side to an image side along an optical axis, a first lens having negative refractive power; a first prism including an incident surface, a reflecting surface, and an exit surface, and an angle between the reflecting surface of the first prism and the optical axis being 45; a stop; a second lens having positive refractive power; a third lens having refractive power; a fourth lens having positive refractive power; a fifth lens having negative refractive power; and a second prism including an incident surface, a reflecting surface, and an exit surface, and an angle between the reflecting surface of the second prism and the optical axis being 45. A total effective focal length f and a maximum field-of-view FOV of the optical imaging lens assembly satisfy: 2.50 mm<f*tan.sup.2(FOV/2)<4.00 mm.

The present disclosure discloses an imaging system and a sequencing system. The imaging system comprises an objective lens and image sensors. The image sensor comprises a first set of image sensors and a second set of image sensors. A first set of tube lenses are arranged between the objective lens and the first set of image sensors and are configured for enabling the imaging system to have a first depth of field when imaging a first surface; a second set of tube lenses are arranged between the objective lens and the second set of image sensors and are configured for enabling the imaging system to have a second depth of field when imaging a second surface. The first depth of field and the second depth of field are both smaller than a distance of displacement from the first surface to the second surface along the optical axis of the objective lens. The imaging system of the present disclosure can simultaneously achieve the acquisition of optical signals on two surfaces of a sample of interest and imaging. This allows the imaging system to detect biomolecules on the two surfaces of the sample of interest, identify the type of bases in the biomolecules, and acquire the base sequences, thus reducing the detection time and improving the detection efficiency and the sequencing throughput.

The present disclosure discloses an optical imaging lens assembly including, sequentially from an object side to an image side along an optical axis, a first lens having negative refractive power; a prism having an incident surface, a reflecting surface, and an exit surface, and an angle between the reflecting surface and the optical axis being 45; a stop; a second lens having positive refractive power; a third lens having refractive power; a fourth lens having positive refractive power; and a fifth lens having negative refractive power.

An imaging optical system includes, in order from an object side to an image side, a first lens group, a second lens group, a third lens group having a positive refractive power, a fourth lens group having a positive refractive power, and a fifth lens group having a negative refractive power, in which, when focusing from an infinite distance object to a close distance object, the first lens group remains stationary with respect to an image surface, the second lens group moves to the object side along an optical axis, the third lens group remains stationary with respect to the image surface, the fourth lens group moves to the object side along the optical axis, and the fifth lens group remains stationary with respect to the image surface, the imaging optical system includes an aperture diaphragm, and the imaging optical system satisfies a predetermined conditional expression.

A variable magnification optical system (ZL) comprises a preceding lens group (GA) having negative refractive power and a succeeding lens group (GB) having positive refractive power, which are arranged in order from the object side along an optical axis. The succeeding lens group (GB) has a focusing group (GF) and an image-side group (GC) disposed closer to the image side than the focusing group (GF), the focusing group (GF) moves to the image side along the optical axis from focusing on an object at infinity to focusing on a close-distance object, and the following conditional expression is satisfied.
1.80<fF/fBaw
FNow<3.40 where fF is the focal length of the focusing group (GF), fBaw is the focal length in the wide-angle end state of an image-side lens group (GBa) composed of lenses disposed on the image side from the focusing group (GF), and FNow is the F-number of the variable magnification optical system (ZL) in the wide-angle end state.