A holographic microscope configured to observe a sample is provided. The holographic microscope includes a light source, a light splitting element, a polarizing element, a phase modulation element, a light combining element, and a photosensitive element. The light source is configured to provide an illumination beam. The illuminating beam is transmitted through the light splitting element to form a first light beam and a second light beam, and the sample is disposed on a transmission path of the first light beam. The polarizing element and the phase modulation element are disposed on the transmission path of the first light beam or the second light beam. The first light beam and the second light beam are transmitted to the light combining element to form an interference beam. The photosensitive element is disposed on a transmission path of the interference beam to receive the interference beam to generate an optical signal.

Provided are an imaging system and an imaging device capable of generating a super-resolution interference fringe image of an object to be observed flowing through a flow channel. A light source that irradiates light in a first direction and irradiates light toward a flow channel through which an object to be observed flows in a second direction orthogonal to the first direction, an imaging sensor that has an imaging surface orthogonal to the first direction and on which a plurality of pixels are two-dimensionally arranged in a manner non-parallel to the second direction and that images light passing through the flow channel to output an interference fringe image, and an information processing device that generates a super-resolution interference fringe image based on a plurality of interference fringe images output from the imaging sensor are included.

A method having the steps of obtaining prepared image data captured by an image sensor receiving light propagated across a sample volume, containing a fluid possibly comprising microscopic objects of foreign origin, while illuminating the sample volume by coherent light. The prepared image data comprising, for a microscopic object, a prepared hologram pattern with prepared spatially alternating intensity formed by the interference fringes; providing filtered image data, comprising automatically filtering the prepared image data by an edge enhancing filter. the filtered image data comprising, for a prepared hologram pattern, a filtered hologram pattern. The presence of the microscopic object associated with the filtered hologram pattern in the sample volume of the fluid is automatically detected on the basis of the filtered hologram pattern.

A method of training AI for label-free cell viability determination includes a step of providing a cell sample, a step of obtaining a fluorescence image and a DHM image of the cell sample, a step of determining a first cell viability of the cell sample according to the fluorescence image of the cell sample, a step of labeling the DHM image of the cell sample as a model specifying the first cell viability, and a step of performing AI training by using the model containing the DHM image of the cell sample.

In an embodiment, the present disclosure pertains to a method of performing circulating tumor cell (CTC) analysis. In general, the method includes flowing a sample through a CTC microfluidic platform, deforming a CTC within the sample, measuring CTC deformation through an imprint of the deformed CTC, processing data related to the measuring, and at least one of identifying or characterizing parameters related to the data that enables at least one of detection of CTCs, enumeration of CTCs in the sample, characterization of biophysical properties, CTC cell size, CTC cell membrane deformability, stresses on CTC cell membranes, adhesion stress on CTC cells, normal stress of CTC cells, or combinations thereof. In some embodiments, the flowing includes passing the sample through at least one channel of the CTC microfluidic platform having a constricted section.

A holographic microscopy characterization (HMC) process for utilizing holographic video microscopy to provide an efficient, automated, label-free method of accurately identifying cell viability. Optical properties of a sample of cells are determined by HMC. The optical properties are compared to known samples or compared over time to observe changes in the optical properties, enabling identification of cells as viable or not viable, or as extra-cellular or degraded cellular materials.

Disclosed is a method of measuring red blood cell membrane fluctuations based on dynamic cell parameters using a digital holographic microscope; the method including a step of modeling the three-dimensional images of red blood cells to be measured, and a step of measuring red blood cell membrane fluctuations based on the three-dimensional images. According to this method, since the three-dimensional images of red blood cells to be measured are modeled and red blood cell membrane fluctuations are measured based on the three-dimensional images, red blood cell membrane fluctuations can be measured more easily.

Apparatus for detecting a 3D structure of an object, comprising at least three laser emitters and a beam splitter that splits the laser radiation of the laser emitters into a reference radiation and an illumination radiation. The illumination radiation strikes the object to be measured, is reflected by the object as object radiation and interferes with the reference radiation. A detector receives the interference patterns formed from the interference of the reference and object radiation and an analysis unit analyzes the interference patterns. At least two of the laser emitters emit laser radiation in the invisible range and the analysis unit detects the object in three dimensions based on the interference patterns of the invisible laser radiation. At least one of the laser emitters emits colored laser radiation and the analysis unit deduces the object's color based on the intensity of the colored object radiation reflected by the object.

Methods, systems and computer program products for performing visual quality assessment using holographic interferometry are provided. Aspects include obtaining a reference holographic pattern based on a reference object and obtaining a test holographic pattern based on a test object. Aspects also include creating an interference pattern by superimposing the test holographic pattern onto the reference holographic pattern. Aspects further include determining a difference between the reference object and the test object based upon the interference pattern.

Systems and methods for uniquely identifying fluid-phase products by endowing them with fingerprints composed of dispersed colloidal particles, and by reading out those fingerprints on demand using Total Holographic Characterization. A library of chemically inert colloidal particles is developed that can be dispersed into soft materials, the stoichiometry of the mixture encoding user-specified information, including information about the host material. Encoded information then can be recovered by high-speed analysis of holographic microscopy images of the dispersed particles. Specifically, holograms of individual colloidal spheres are analyzed with predictions of the theory of light scattering to measure each sphere's radius and refractive index, thereby building up the distribution of particle properties one particle at a time. A complete analysis of a colloidal fingerprint requires several thousand single-particle holograms and can be completed in ten minutes.