The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

A holographic optical tweezers for manipulating a micro- or nano-size particle, the optical tweezers including a light source configured to emit first and second light beams; a light focusing apparatus configured to focus the first and second light beams to generate focused light beams, which create optical forces; and a trapping assembly configured to receive the first and second focused light beams and form a trap for holding the particle with the optical forces. The trapping assembly includes first and second micromirrors attached to a microscope coverslip.

The invention discloses an optical system for generating arbitrary-order optical vortex arrays and finite optical lattices with defects, comprising a laser, a collimating and beam-expanding system, a spatial light modulator, a 4-f lens system, and an image detector which are disposed according to a light path. After passing through the collimating and beam-expanding system, the linearly-polarized Gaussian beam emitted by the laser is radiated to the spatial light modulator to be modulated in complex amplitude; the first-order diffraction beam of the emergent light generates an arbitrary-order alternating optical vortex array on the back focal plane of the first 2-f lens system, and an adjustable finite optical lattice with defects on the back focal plane of the second 2-f lens system. The topological charge value of each vortex and the spacing between vortices, in the generated arbitrary-order alternating optical vortex array, can be precisely controlled.

An optical image generation system including: a spatial light modulator (SLM) configured to receive an input collimated laser beam and modulate the wavefront of the laser beam; one or more optical elements configured to project the modulated laser beam onto a focal plane; a first mirror and a second mirror situated at the focal plane, an edge of the first mirror being adjacent to an edge of the second mirror, the first mirror reflects a first portion of the modulated laser beam in a first direction, the second mirror reflects a second portion of the modulated laser beam in a second direction; and an objective lens projects the first and second portions into a combined image; wherein the zeroth order diffraction is block or suppressed at the center of the focal plane.

The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

A method and a device for measuring light field distribution are provided; including steps of utilizing the optical trap to stably levitating particles, moving the optical trap to bring the particles close to the light field to be measured, and utilizing the photodetector to collect the scattered light signals of the particles at different positions in the three-dimensional space of the light field to be measured, and calculating the light field distribution of the light field to be measured according to the scattered light intensity which is proportional to the light intensity at that position. The device for measuring the optical field distribution includes a laser, an optical trapping path, particles, a photodetector, a control system and an upper computer; the laser emits a laser, passes through the optical trapping path, and emits highly focused captured light B to form an V optical trap to capture particles.

The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

Apparatus and methods for 3D-Scanless Holographic Optogenetics with Temporal focusing (3D-SHOT), which allows precise, simultaneous photo-activation of arbitrary sets of neurons anywhere within the addressable volume of the microscope. Soma-targeted (ST) optogenetic tools, ST-ChroME and IRES-ST-eGtACR1, optimized for multiphoton activation and suppression are also provided. The methods use point-cloud holography to place multiple copies of a temporally focused disc matching the dimensions of a designated neuron's cell body. Experiments in cultured cells, brain slices, and in living mice demonstrate single-neuron spatial resolution even when optically targeting randomly distributed groups of neurons in 3D.

Atoms and atom-like quantum emitters are promising for quantum sensing, computing, and communications. Lasers and microscopes enable high-fidelity quantum control of the atomic quantum bits (qubits). However, it is challenging to scale up individual quantum control to enough atomic quantum nodes for implementing useful and practical quantum algorithms. Here, we introduce methods and systems to holographically implement large-scale quantum circuits that individually address atomic quantum nodes. These methods enable implementation of quantum circuits over large, multi-dimensional arrays of atomic qubits at rates of thousands to millions of quantum circuit layers per second. The quantum circuit layers are encoded in multiplexed holograms displayed on a slow SLM and retrieved by fast interrogation to produce spatial distributions that operate on the qubit array. This technology can also be used for optically addressing objects such as biological cells and on-chip photonic components for optical tweezers, opto-genetics, optical computing, and optical neural networks.