A method of generating a color image of a sample includes obtaining a plurality of low resolution holographic images of the sample using a color image sensor, the sample illuminated simultaneously by light from three or more distinct colors, wherein the illuminated sample casts sample holograms on the image sensor and wherein the plurality of low resolution holographic images are obtained by relative x, y, and z directional shifts between sample holograms and the image sensor. Pixel super-resolved holograms of the sample are generated at each of the three or more distinct colors. De-multiplexed holograms are generated from the pixel super-resolved holograms. Phase information is retrieved from the de-multiplexed holograms using a phase retrieval algorithm to obtain complex holograms. The complex hologram for the three or more distinct colors is digitally combined and back-propagated to a sample plane to generate the color image.

A Volume Bragg grating (VBG) is placed in the back of a collimating lens, the incident angle between exposure light beam and the Volume Bragg grating is equal to the Bragg angle of the Volume Bragg grating. A photodetector is placed in the 1 grade transmission diffraction light path of the Volume Bragg grating which the exposure light beam is emitted to. The pinhole filter is moved back and forth along an optical axis and the reading of the photodetector is observed in real time. When the reading of the photodetector is maximum, fix the pinhole filter and keep the distance between the first pinhole filter and the first collimating lens a constant. The method for adjusting the self-collimation optical path is provided, using the Volume Bragg grating to detect the parallelism of self-collimation light and substituting for a traditional Moire pattern adjustment method.

A method of generating a color image of a sample includes obtaining a plurality of low resolution holographic images of the sample using a color image sensor, the sample illuminated simultaneously by light from three or more distinct colors, wherein the illuminated sample casts sample holograms on the image sensor and wherein the plurality of low resolution holographic images are obtained by relative x, y, and z directional shifts between sample holograms and the image sensor. Pixel super-resolved holograms of the sample are generated at each of the three or more distinct colors. De-multiplexed holograms are generated from the pixel super-resolved holograms. Phase information is retrieved from the de-multiplexed holograms using a phase retrieval algorithm to obtain complex holograms. The complex hologram for the three or more distinct colors is digitally combined and back-propagated to a sample plane to generate the color image.

A device and method for recording and reproducing holographic information, and a display apparatus are disclosed. The device for recording holographic information comprises an electro-optical regulating element, which is arranged in a light path between an object to be reproduced and a recording medium, receives an object beam scattered by the object to be reproduced, and regulates an outgoing direction and a focal distance of the object beam to irradiate the recording medium. The recording medium is configured to receive the reference beam from the light source and the object beam regulated by the electro-optical regulating element. The reference beam and the object beam form information relevant with the object to be reproduced in the recording medium. By regulating the outgoing direction and focal distance of beam with the electro-optical regulating element, it is unnecessary to rotate the recording medium for recording and reproducing the holographic information.

A method of imaging a sample having birefringent crystals (or other materials) using a lens-free polarized microscopy device includes illuminating the sample contained on a sample holder with circularly polarized partially coherent or coherent light and capturing lower resolution holographic images of the birefringent crystals with an image sensor. A polarization analyzer unit made from a /4 retarder and a linear polarizer is positioned between the sample holder and the image sensor. The lower resolution holographic images are obtained with the polarization analyzer unit in two different orientations (e.g. 90 orientations). Phase-retrieved, higher resolution images of the birefringent crystals at the different orientations are obtained using the lower resolution holographic images. A differential image is generated from the respective phase-retrieved, higher resolution images. An object support mask is applied to identify the birefringent crystals which can then be pseudo-colored.

Systems and methods for sub-aperture correlation based wavefront measurement in a thick sample and correction as a post processing technique for interferometric imaging to achieve near diffraction limited resolution are described. Theory, simulation and experimental results are presented for the case of full field interference microscopy. The inventive technique can be applied to any coherent interferometric imaging technique and does not require knowledge of any system parameters. In one embodiment of the present application, a fast and simple way to correct for defocus aberration is described. A variety of applications for the method are presented.

In one embodiment, a self-interference incoherent digital holography system including a light sensor and a diffractive filter configured to receive light from an object to be holographically imaged and generate holographic interference patterns on the light sensor. A self-interference incoherent digital holography system comprising: a light sensor; and a diffractive filter configured to receive light from an object to be holographically imaged and generate holographic interference patterns on the sensor.

An optical system comprises an imaging holographic optical element, which produces a floating hologram. An upstream light-forming holographic optical element causes spectral filtering of the light.

A system and method to produce a hologram of a single plane of a three dimensional object includes an electromagnetic radiation assembly to elicit electromagnetic radiation from a single plane of said object, and an assembly to direct the elicited electromagnetic radiation toward a hologram-forming assembly. The hologram-forming assembly creates a hologram that is recorded by an image capture assembly and then further processed to create maximum resolution images free of an inherent holographic artifact.

The present invention relates to an improved method for determining the identity of a biomolecule using interferometric light scattering apparatus, notably distinguishing between variants of biomolecules, such as viral serotypes and the like. Such a method has potential to simplify the testing for biological manufacture and for diagnosing disease, monitoring environmental issues and determination of contaminants.