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GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
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Water conservation; Efficient water supply; Efficient water use
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METHOD FOR PRODUCING PANCREATIC BLAST CELLS AND PANCREATIC DISEASE TREATMENT AGENT CONTAINING PANCREATIC BLAST CELLS
20170175082 · 2017-06-22 ·

Provided is a method for generating pancreatic bud cells, having the step of culturing PDX1.sup.+/NKX6.1.sup. cells in a medium containing KGF, EGF and a BMP inhibitor. The culturing step may be performed in suspension cultures or in adherent cultures. When the cells are cultured in adherent cultures, the cells may be cultured in a medium further containing a ROCK inhibitor or a nonmuscle myosin II inhibitor.