IMPROVED PROCESS FOR THE PREPARATION OF IMMUNOGLOBULIN G (IgG)
20170247433 · 2017-08-31
Inventors
Cpc classification
International classification
Abstract
The present invention is directed to processes for extracting IgG from an unused waste precipitate produced during normal plasma fractionation processes via a separate fractionation process, thereby increasing the overall yield of IgG from blood plasma.
Claims
1. A method for extracting IgG from a waste precipitate fraction, the method comprising contacting the waste precipitate fraction with a suitable solvent to extract IgG from the precipitate, wherein the waste precipitate fraction is produced during plasma fractionation and separated from the main IgG manufacturing process stream.
2. A method for improving the yield of IgG during plasma fractionation to produce IgG, the method comprising extracting IgG from a waste precipitate fraction, wherein the waste precipitate fraction is produced during plasma fractionation and separated from the main IgG manufacturing process stream using a suitable solvent.
3. A method for the separation of IgG from plasma or a plasma fraction, the method comprising a) fractionating the plasma or plasma fraction to produce a liquid fraction containing a majority of the IgG present in the plasma or plasma fraction, and a waste precipitate fraction containing additional IgG; and b) extracting at least some of the additional IgG from the waste precipitate fraction using a suitable solvent.
4. A method according to claim 1, wherein the waste precipitate fraction is produced during a fractionation step to produce a liquid fraction (supernatant) containing IgG.
5. A method according to claim 4, wherein the liquid fraction is selected from the group consisting of Cohn supernatant III, Cohn supernatant I+III, Kistler & Nitschmann supernatant B, Kistler & Nitschmann supernatant B+I, modified Kistler & Nitschmann precipitate B, and Kistler & Nitschmann B+I.
6. A method according to claim 1, wherein the waste precipitate fraction is selected from the group consisting of Cohn supernatant III, Cohn supernatant I+III, Kistler & Nitschmann supernatant B, Kistler & Nitschmann supernatant B+I, modified Kistler & Nitschmann precipitate B, and Kistler & Nitschmann B+I.
7. A method according to claim 1, wherein the solvent is buffered aqueous ethanol.
8. A method according to claim 7, wherein the solvent is an aqueous buffer containing about 13 vol. % to about 17 vol. % ethanol.
9. A method according to claim 7, wherein the solvent is buffered using phosphate and/or acetate buffer.
10. A method according to claim 1, wherein the ratio of waste precipitate fraction to solvent is from about 1:2 to about 1:10.
11. A method according to claim 1, wherein the solvent containing extracted IgG is separated from any remaining waste precipitate solids.
12. A method according claim 11, wherein the solvent containing extracted IgG is separated from remaining waste precipitate solids by filtration or centrifugation.
13. A method according to claim 1, wherein the extracted IgG is combined with IgG obtained from the main IgG manufacturing process stream.
14. A method according to claim 1, wherein the method for extracting IgG from the waste precipitate fraction does not involve any chromatographic steps.
15. A method according to claim 1, wherein the extracted IgG obtained from the waste precipitate is further processed to produce a pharmaceutical IgG product.
16. A method according to claim 15, wherein the further processing to produce a pharmaceutical IgG product comprises precipitation of the extracted IgG from the solvent.
17. A method according to claim 16, wherein the precipitation is achieved by adjusting one or more of ethanol concentration, temperature and pH.
18. A method according to claim 16, wherein the precipitation yields Fraction II.
19. A method according to claim 16, wherein the resulting precipitate is combined with an IgG rich fraction from the main IgG manufacturing process stream.
20. A method for the preparation of IgG comprising: a) recovering precipitate and supernatant from a modified Kistler and Nitschmann B+I fractionation process; b) homogenising the precipitate obtained in step a) and extracting IgG therefrom by mixing with 17 vol. % aqueous ethanol in acetate/phosphate buffer at 0° C. for 1 to 3 hours; and then c) separating the buffer containing extracted IgG from any remaining precipitate.
21. The method according to claim 20, further comprising: d) combining the buffer containing extracted IgG obtained in step c) with the modified Kistler and Nitschmann B+I supernatant obtained in step a).
22. The method according to claim 20, wherein step c) is performed via filtration and wherein the method further comprises: e) incubating the extracted IgG in aqueous ethanol according to the conditions for modified Kistler and Nitschmann Fraction II precipitation; and f) recovering the resulting IgG-enriched Fraction II precipitate.
23. A method according to claim 1, wherein the extracted IgG product comprises not less than 85 wt. % monomer and dimer and not more than 10 wt. % polymer and/or aggregate.
24. A method according to claim 1, wherein the extracted IgG product comprises a purity of not less than 90% gammaglobulin.
Description
BRIEF DESCRIPTION OF THE FIGURES
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[0040]
[0041]
DETAILED DESCRIPTION
[0042] In the following, the terms “liquid fraction” and “supernatant” are equivalent, as are the terms “precipitate” and “fraction”.
[0043] The IgG products obtained in the methods described herein are of high purity, with minimal aggregated IgG, and therefore meet the minimum standards for purity set out the European Pharmacopoeia monograph 0338 (January 2015). Thus, the IgG products directly obtained by the methods described herein contain at least 90% gammaglobulin, as determined by electrophoresis. The product obtained by these methods also contains at least 85 wt. % monomeric and dimeric IgG, with less than 10 wt. % of polymeric and aggregated IgG, as determined by size exclusion chromatography. HPLC may also be used to analyse aggregate content. This means that the products obtained from the fractionation processes described herein are of sufficient purity and quality to meet European pharmacopoeial standards, and therefore do not necessarily require any further purification to form a pharmaceutical product. Of course, the IgG obtained by the fractionation processes described herein may be further processed by any suitable means to obtain a higher quality IgG product, or to obtain a different product profile e.g. for an alternative pharmaceutical use. Such further processing methods are well known to the person skilled in the art, and may include in particular one or more virus inactivation steps.
[0044] Cold ethanol fractionation is one of the most widely used methods for isolating IgG from blood plasma. Generally, a pooled batch of plasma from multiple donors is subjected to cryoprecipitation to remove clotting factors such as Factor VIII as cryoprecipitate. The cryoprecipitate supernatant is then subjected to one or more cold ethanol fractionation steps to eventually produce a precipitate fraction comprising primarily IgG. In both the classic Cohn/Oncley process and the Kistler & Nitschmann method, this IgG-rich fraction is referred to as Fraction II or precipitate II. This precipitate is then subjected to further purification and virus inactivation steps to provide a pharmaceutically acceptable IgG final product, for intravenous, subcutaneous or intramuscular infusion.
[0045] “Normal plasma”, “hyperimmune plasma” (such as hyperimmune anti-D, tetanus or hepatitis B plasma) or any plasma equivalent thereto can be used in the cold ethanol fractionation processes described herein.
[0046] In the Cohn fractionation method, the first fractionation step results in fraction I which comprises mainly fibrinogen and fibronectin. The supernatant from this step is further processed to precipitate out fraction II+III and then fractions III and II. Typically, fraction II+III contains approximately 60% IgG, together with impurities such as fibrinogen, IgM, and IgA. Most of these impurities are then removed in fraction III, which is considered a waste fraction and is normally discarded. The supernatant is then treated to precipitate out the main IgG-containing fraction, fraction II, which can contain greater than 90% IgG. The above % values refer to % purity of the IgG. Purity can be measured by any method known in the art, such as gel electrophoresis.
[0047] In the Kistler & Nitschmann method, fraction I is equivalent to fraction I of the Cohn method. The next precipitate/fraction is referred to as precipitate A (fraction A). This precipitate is broadly equivalent, although not identical, to Cohn fraction II+III. The precipitate is then redissolved and conditions adjusted to precipitate out precipitate B (fraction B), which is equivalent to Cohn fraction III. Again, this is considered to be a waste fraction, and is normally discarded. The precipitate B supernatant is then processed further to produce precipitate II, which corresponds to Cohn Fraction II.
[0048] In a modification of the Kistler & Nitschmann process, shown in
[0049] In principle, the methods of the present invention may be applied to any of the precipitate fractions produced during cold ethanol fractionation prior to production of fraction II, including Cohn fraction III and fraction I+III and Kistler & Nitschmann precipitate B or B+I. Preferably, the methods are applied to the precipitate fraction immediately preceding fraction II in the fractionation process, which is normally considered a waste fraction. Preferably, the waste precipitate fraction is Cohn fraction III or I+III, Kistler & Nitschmann precipitate B or B+I, a modified Kistler & Nitschmann precipitate B or B+I, or a fraction which is substantially equivalent thereto.
[0050] It has been found that these waste precipitate fractions can contain significant amounts of the IgG from the starting plasma pool, in some cases as much as 25-30%. This is postulated to be due in part to some supernatant being trapped in the precipitate fraction and in part due to co-precipitation of IgG with impurities such as IgM and IgA.
[0051] Surprisingly, it has been found that it is possible to recover a commercially significant proportion of the “lost” IgG in relatively pure form through a simple washing (extraction) process applied to the waste fraction. This result is unexpected, as a skilled person would have expected that extracting IgG from waste precipitate would be difficult given the relatively high levels of other proteins present in the precipitate, and that any IgG that could be extracted would be of low purity (e.g. in terms of aggregation and other unwanted protein species or proteolytic activity).
[0052] It is important to note that the method of the present invention does not require any chromatographic steps to extract further IgG from the waste fraction. Thus, the process of the present invention provides a relatively pure IgG product from waste fractions without the need for chromatography.
[0053] Choice of a suitable solvent for the washing process has been found to allow extraction of IgG from the waste precipitate without significant extraction of any of the other proteins present in the precipitate, which are considered to be impurities insofar as IgG purification is concerned. Thus, the solvent should be selected to be specific for removal of IgG from the precipitate, without simultaneously removing unwanted impurities from the precipitate.
[0054] The waste precipitate fraction can be subjected to a washing process immediately after it is produced. Alternatively, the waste fraction can be stored in frozen form for later processing. Before washing, the precipitate should, if necessary, be equilibrated to the temperature at which the washing process will be carried out. Such equilibration will generally be static, i.e. will not involve any agitation of the precipitate.
[0055] The type and amount of solvent used for the washing process should be selected such that recovery of IgG is maximised without unduly compromising the purity of the recovered IgG. To optimise IgG recovery whilst minimising extraction of impurities from the waste precipitate, the solvent used for the washing/extraction is preferably identical to the solvent used in the fractionation step which produced the waste precipitate in question.
[0056] When the waste fraction is (modified) Kistler & Nitschmann precipitate B or B+I, or Cohn fraction III or I+III preferred solvents are aqueous ethanol solutions. More preferably, such ethanol solutions are buffered. The ethanol concentration, temperature and pH should be controlled such that IgG extracted from the precipitate remains in solution.
[0057] Preferred concentrations of ethanol are in the range of about 10 to about 20 vol. %. More preferred is a concentration range of about 11 to about 19 vol. %, even more preferred is a concentration range of about 12 to about 19 vol. %, most preferably a concentration range of about 13 to about 17 vol. %. About 13 vol. % and about 17 vol. % are most preferred. “About 13 vol. %” is preferably 13±2 vol. %, whilst “about 17 vol. %” is preferably 17±2 vol. %.
[0058] The temperature at which the washing process occurs will also affect the amount and purity of the IgG recovered. Ideally, the temperature during the washing process is maintained at the same temperature that was used for the fractionation step which produced the waste precipitate in question. The optimum temperature range will therefore depend on the fraction and the fractionation process in question. Generally preferred are temperatures in the range of about −3° C. to about −8° C., including about −3° C. to about −7° C. For example, when the waste fraction is (modified) Kistler & Nitschmann precipitate B or B+I, the temperature during the washing extraction is preferably −5° C.±2.0° C. When the waste fraction is Cohn fraction III or I+III, the preferred temperature is usually slightly cooler, preferably −6° C.±2.0° C.
[0059] Generally preferred are temperatures in the range of about +3° C. to about −8° C., including about −3° C. to about −7° C. For example, when the waste fraction is modified Kistler & Nitschmann precipitate B or B+I, the temperature during the washing extraction is preferably −2° C.±5° C. When the waste fraction is Cohn fraction III or I+III, the preferred temperature is usually slightly cooler, preferably −3° C.±5.0° C.
[0060] The optimum pH range will also depend on the fraction. Generally preferred are pHs in the range of about 4.8 to about 5.3, more preferably about 5.1 to about 5.3. For example, when the waste fraction is (modified) Kistler & Nitschmann precipitate B or B+I, the pH range is preferably 5.1±0.05. When the waste fraction is Cohn fraction III or I+III, the pH may be slightly higher, preferably 5.2±0.05.
[0061] In a particularly preferred embodiment, when the waste fraction is (modified) Kistler & Nitschmann precipitate B or B+I, the solvent is 17±2 vol. % aqueous ethanol, the temperature is −5° C.±2.0° C. and the pH range is 5.1±0.05.
[0062] In a particularly preferred embodiment, when the waste fraction is (modified) Kistler & Nitschmann precipitate B or B+I, the solvent is 17±2 vol. % aqueous ethanol, the temperature is −2° C.±5° C. and the pH range is 5.1±0.05.
[0063] In another particularly preferred embodiment, when the waste fraction is Cohn fraction III or I+III, the solvent is 17±2 vol. % aqueous ethanol, the temperature is −6° C.±2.0° C. and the pH range is 5.2±0.05.
[0064] In another particularly preferred embodiment, when the waste fraction is Cohn fraction III or I+III, the solvent is 17±2 vol. % aqueous ethanol, the temperature is −3° C.±5° C. and the pH range is 5.2±0.05.
[0065] In another particularly preferred embodiment, when the waste fraction is (modified) Kistler & Nitschmann precipitate B or B+I prepared from hyperimmune plasma, or equivalent plasma, the solvent is 13±2 vol. % aqueous ethanol, the temperature is −5° C.±2.0° C. and the pH range is 5.1±0.05.
[0066] In another particularly preferred embodiment, when the waste fraction is (modified) Kistler & Nitschmann precipitate B or B+I prepared from hyperimmune plasma, or equivalent plasma, the solvent is 13±2 vol. % aqueous ethanol, the temperature is −2° C.±5° C. and the pH range is 5.1±0.05.
[0067] In another particularly preferred embodiment, when the waste fraction is Cohn fraction III or I+III prepared from hyperimmune plasma, or equivalent plasma, the solvent is 13±2 vol. % aqueous ethanol, the temperature is −6° C.±2.0° C. and the pH range is 5.2±0.05.
[0068] In another particularly preferred embodiment, when the waste fraction is Cohn fraction III or I+III prepared from hyperimmune plasma, or equivalent plasma, the solvent is 13±2 vol. % aqueous ethanol, the temperature is −3° C.±5° C. and the pH range is 5.2±0.05.
[0069] The solvent may be buffered using known buffers, including phosphate and acetate.
[0070] Generally, the washing step comprises suspension of the waste fraction in the solvent. The suspension is mixed, ideally until it is homogenised, and then left for a sufficient period of time for IgG to be extracted into the solvent. This corresponds to the “extract” and “condition” steps shown in
[0071] In a preferred aspect, the invention provides a method for the preparation of IgG comprising: [0072] a) recovering precipitate and supernatant from a modified Kistler and Nitschmann B+I fractionation process; [0073] b) homogenising the precipitate obtained in step a) and extracting IgG therefrom by mixing with 17 vol. % aqueous ethanol in acetate/phosphate buffer at 0° C. for 1 to 3 hours; and then [0074] c) separating the buffer containing extracted IgG from any remaining precipitate.
[0075] More preferably, the method above further comprises combining the buffer containing extracted IgG obtained in step c) with the modified Kistler and Nitschmann B+I supernatant obtained in step a). Alternatively, in the method described above, step c) is performed via filtration and the method further comprises incubating the extracted IgG in aqueous ethanol according to the conditions for modified Kistler and Nitschmann Fraction II precipitation; and recovering the resulting IgG-enriched Fraction II precipitate.
[0076] The IgG rich solution produced by the washing/extraction step preferably contains IgG of the same or similar purity to the main IgG-containing supernatant produced when the waste fraction was precipitated and/or separated away from the established IgG target protein manufacturing process stream.
[0077] Given the commercial and therapeutic value of IgG, any improvement in IgG yield from the starting plasma is potentially important, and even relatively low % recoveries of IgG from a waste fraction can be highly valuable.
[0078] The resulting IgG enriched solution may be recovered by any standard method known in the art, for example centrifugation or filtration to separate it from the remaining precipitate. If centrifugation is used, the supernatant will be IgG rich (i.e. will contain the extracted IgG), and the precipitate may be discarded, treated again according to the invention to extract further IgG and/or used for the extraction of other proteins. If filtration is used, the filtrate will be IgG rich, and the resultant filter cake may be discarded, further flushed to recover residual entrained IgG, treated again according to the invention to extract further IgG and/or used for the extraction of other proteins. Suitable filter media are known in the art. A silicate filter aid such as kieselghur, for example CELITE® or CELPURE®, may be added to facilitate filtration.
[0079] Any volume of solvent may potentially be used in the wash process, but should ideally be optimised for the available processing equipment. If very low volumes are used, the resulting suspension may be too viscous to process easily whilst very high volumes could lead to process inefficiencies. For reasons of process efficiency, it is therefore generally preferred to keep the volume of solvent relatively low. For example, the weight of waste fraction to solvent will generally be from about 1:2 to about 1:10. Preferably, the weight of solvent may be approximately four times the weight of the waste fraction, i.e. a weight ratio of about 1:4 of waste fraction to solvent.
[0080] The IgG rich solutions obtained using the processes of the invention can be further processed by methods known in the art to provide a pharmaceutically acceptable IgG product, according to the standards set by both the US and European Pharmacopoeia. Preferably, standard conditions are used to precipitate Fraction II from the solution, which is then further purified to provide a pharmaceutical product such as intravenous immunoglobulin (IVIG) or subcutaneous immunoglogulin (SCIG).
[0081] Further purification may take the form of anion and/or cation exchange chromatography, combined with suitable steps to assure the virus safety of the IVIG or SCIG (see Roberts et. al., Biologicals, Vol. 43(2), March 2015, p123-129).
[0082] The IgG rich solution resulting from extraction from the waste fractions can be processed separately, combined with other solvent extraction of the same waste fractions and/or combined with the solvent extraction of the waste fractions from other process batches. However, it is generally more efficient to combine these solutions with the bulk IgG process intermediate of the main target protein downstream process. For example, the IgG from solvent extraction of the “waste fraction” may be combined with the IgG-rich supernatant from the fractionation step which produced the relevant waste precipitate. This may be the supernatant from the same fractionation batch, or a different batch.
[0083] Alternatively, the IgG extracted from the waste fraction may undergo one or more of the same downstream manufacturing steps as are used in the main IgG process, before being combined with the bulk IgG intermediate at a downstream process stage. For example, the IgG rich solution produced by washing/extraction from precipitate B+I may be combined with B+I supernatant which is then processed further to the final product. Alternatively, the IgG rich solution produced by washing/extraction from precipitate B+I may be processed through downstream process steps such as ethanol precipitation to Fraction II, before combining with the main IgG Fraction II intermediate processed from B+I supernatant and processing to the final product. Either option may be preferred, depending on the available scale of manufacturing equipment and associated logistics. The optimum solvent to be used for the extraction is may depend in part on any intended subsequent processing step(s).
[0084] Various alternative points at which the extracted IgG may be recombined with the main IgG process stream are shown in
[0085] Preferred features of the invention may be combined in any manner. Thus certain features which are, for clarity, described herein in the context of separate embodiments, may be combined in any manner. Conversely, various features that are, for brevity, described in the context of a single preferred feature, may also be provided separately or in any subcombination. Further, reference to values stated in ranges includes each and every value within that range.
[0086] Note that not all of the activities described above in the general description are required, that a portion of a particular activity may not be required, and that one or more further activities may be performed in addition to those described. Still further, the order in which activities are listed are not necessarily the order in which they are performed.
EXAMPLES
[0087] The following non-limiting Examples further illustrate the present invention.
[0088] In the following Examples/Tables, the precipitates and supernatants were analysed for protein content using a ‘SpaPlus’ auto-analyser (The Binding Site, Birmingham, UK), which is a turbidometric analysis platform. Coagulation factors VII, IX, XI and XII were analysed using AssayPro ELISA kits (supplied by Universal Biologicals, Cambridge, UK). Factor XIa was measured using the Hyphen BioPhen chromogenic assay kit (supplied by Quadratech Diagnostics, Epsom, UK).
[0089] Table 1 below shows a typical composition of a B+I precipitate that is a waste fraction (equivalent to Cohn/Oncley Fraction III) produced by Kistler & Nitschmann cold-ethanol fractionation.
TABLE-US-00001 TABLE 1 B + I precipitate mg/litre Protein plasma % total alpha 1 acid glycoprotein 7 0.1 alpha 1 antichymotrypsin 31 0.5 C1 inactivator 12 0.2 Ceruloplasmin 195 3.2 Antithrombin III 77 1.3 Prealbumin 78 1.3 B2 glycoprotein 1 16 0.3 Gc Globulin 51 0.8 alpha 2 macroglobulin 1240 20.6 Haemopexin 12 0.2 alpha 1 antitrypsin 51 0.8 IgA 1000 16.6 IgG 1100 18.3 IgM 710 11.8 alpha 2 HS glycoprotein 27 0.4 Haptoglobin 65 1.1 Albumin 204 3.4 Fibrinogen 457 7.6 Apolipoprotein B 506 8.4 Inter alpha trypsin inhibitor 168 2.8 Transferrin 9 0.1 Total Protein 6016 100
[0090] Starting material for the experiments described below was an IgG containing precipitate B+I, which had a composition similar to that shown in Table 1.
[0091] In the following examples, the 17 vol. % ethanol aqueous buffered solution is made as follows: [0092] disodium hydrogen phosphate dihydrate 7.1 mM (1.27 g/L) [0093] glacial acetic acid 12.8 mM (0.77 g/L)
[0094] 17 vol. % ethanol in phosphate/acetate buffer: 141.6 g of 96% ethanol added to 858.4 g phosphate/acetate buffer (final pH is ˜5.0-5.1).
Example 1
[0095] In a first experiment, 97 g of precipitate B+I was rapidly re-suspended by homogeniser at a pH of 4.8 to 5.2 in the presence of 1,000 g of a 17 vol. % ethanol aqueous buffered solution at a temperature range of about −3° C. to −7° C., to give a precipitate to buffer ratio of 1:10. The buffer consisted of 17 vol. % aqueous ethanol solution containing phosphate and acetate adjusted to the appropriate pH (“ethanol buffer”).
[0096] In a second experiment, 99 g of the same precipitate B+I was re-suspended in the same manner in 1,000 g of 17 vol. % ethanol in water (i.e. unbuffered ethanol solution) at a temperature range of about −3° C. to −7° C. Both re-suspensions were then continuously mixed by moderate agitation and the precipitate was conditioned (matured) under the stated conditions over 24 hours. Samples of the buffered and non-buffered re-suspensions were centrifuged to recover the IgG enriched liquid. The pre-centrifuged suspension and the supernatant were analysed for the presence of IgG and several other proteins of interest (Table 2).
TABLE-US-00002 TABLE 2 Analysis of re-suspended precipitate B + I and supernatant fraction from buffered and unbuffered 17 vol. % aqueous ethanol solution 17 vol. % Ethanol in 17 vol. % Ethanol in Phoshate/Acetate Buffer Water SAMPLES Resuspension Supernatant Resuspension Supernatant Analysis Turbidity (NTU) 23.5 1,233 IgG (mg/mL) 3.47 1.24 3.46 2.23 Albumin (mg/mL) 0.76 0.33 0.81 0.28 IgA (mg/mL) 1.67 0.03 1.94 0.43 IgM (mg/mL) 0.85 <0.11 0.94 0.25 Haptoglobin (mg/mL) 0.06 <0.025 0.06 0.03 FXIa (ng/mL) 153 <0.6 105 8.26 FXI (ng/mL) 228 <0.78 211 17.0 FXII (μg/mL) 25.4 0.87 44.5 2.29 Protease (U/mL) 547 7.22 425 >50 Plasmin (IU/mL) 0.45 0.11 0.40 <0.1 Plasminogen (U/mL) NQ.sup.a 0.09 0.43 0.79 .sup.aNQ = Not Quantifiable - sample OD between blank and lowest point of standard line NTU = Nephelometric Turbidity Ratio Units, measured using a turbiditimeter calibrated in NTU
[0097] From Table 2 it can be seen that re-suspension in both the phosphate/acetate buffered 17 vol. % aqueous ethanol solution (“ethanol buffer”) and the unbuffered ethanol solution successfully extracted IgG. Unbuffered ethanol supernatant extract contained a higher concentration of IgG than the buffered supernatant, but buffered ethanol supernatant extract contained IgG with higher quality and much lower proportion of IgA and IgM. Protease activity, Factor XI/XIa and Factor XII were also lower in the buffered ethanol supernatant extract. High rates of thromboembolic side effects have been related to Factor XI and XIa in IVIG products and it is therefore highly desirable not to re-extract these into the supernatant fraction. Unlike the ethanol/water extract, the ethanol/buffer extract was similar in composition to the Supernatant B+I fraction (which goes on to Fraction II) and therefore admixing of the two fractions is possible to generate a single Fraction II of equivalent purity containing a higher yield of IgG.
[0098] The yield of IgG from the phosphate/acetate buffered aqueous ethanol supernatant was equivalent to 0.55 g of IgG per litre of plasma, which represents a yield increase of ˜10% of the plasma IgG which can translate into 10-20% additional yield of the IgG final product.
Example 2
[0099] In a second set of experiments, the effect of reducing the re-suspension ratio was investigated to reduce the overall volume. In one experiment, 500 g of B+I precipitate was re-suspended in 1000 g of buffered aqueous ethanol solution (one part precipitate to two parts buffer). In another experiment 250 g of the same B+I precipitate was re-suspended in 1,000 g of ethanol buffer (one part precipitate to four parts buffer). Samples were taken at intervals during the maturation for analysis (Table 3).
TABLE-US-00003 TABLE 3 Effect of re-suspension ratio (precipitate:ethanol buffer) on quality of B + I suspension supernatant over 24 hours Maturation Time (Hours) 0.5 2.0 6.5 24 SAMPLES Re-suspension ratio Method 1:2 1:4 1:2 1:4 1:2 1:4 1:2 1:4 Analysis Turbidity 3,432 27.2 3,314 28.1 4,000 47.9 3,765 30.7 (NTU) IgG 4.98 2.69 4.69 2.78 3.97 2.85 4.30 1.83 (mg/mL) Albumin 0.96 0.48 0.87 0.57 0.74 0.63 0.85 0.39 (mg/mL) IgA 0.30 0.02 0.34 0.03 0.37 0.04 0.32 <0.02 (mg/mL) IgM 0.18 <0.11 0.19 <0.11 0.20 <0.11 0.2.0 <0.11 (mg/mL) FXIa 5.20 <0.6 5.20 <0.6 6.65 <0.6 5.85 <0.6 (ng/mL)
[0100] The data in Table 3 indicate that ratios of 1:2 and 1:4 enable extraction of IgG from the precipitate and that the yield and quality of the extracted IgG was greater at the 1:4 ratio. For example, the turbidity of the 1:4 supernatants, which is a gross indicator of material quality, was two orders of magnitude lower than the equivalent 1:2 re-suspension supernatants. In addition, the concentrations of contaminants such as IgM, IgA and Factor XIa, were significantly decreased in 1:4 supernatant compared to 1:2 supernatant. Data from both experimental runs also suggest that the quality and yield of IgG can be maintained using maturation times of approximately two hours or more. The yield of IgG after 2 hour maturation was 0.47 g and 0.42 g of IgG per litre of plasma in the 1:4 and 1:2 supernatants respectively.
Example 3
[0101] Several re-suspensions were carried out at a 1:4 ratio to confirm the results of Example 2. A different batch of starting material was used in six re-suspensions. In each case, 250 g of B+I precipitate was re-suspended in 1,000 g of aqueous ethanol buffer as described above. The precipitate suspension was conditioned at −5° C. whilst mixing for a minimum of 2 hours. At the end of conditioning, the re-suspension was centrifuged. Both the re-suspension and the supernatant were analysed. The IgG concentration and IgG yield (plasma equivalent) of the supernatants were calculated (Table 4).
TABLE-US-00004 TABLE 4 IgG content and yield of confirmatory B + I re-suspensions Confirmatory Runs 1:4 (B + I:Ethanol Buffer) SAMPLE 1 2 3 4 5 6 MEAN IgG (mg/mL) 4.40 2.18 3.07 2.83 2.89 4.73 3.35 Yield: IgG 0.75 0.35 0.52 0.48 0.49 0.8 0.57 g/L PE
[0102] The mean yield of IgG was 0.57 g IgG per litre of plasma. By addition to the main fractionation process stream, this yield can be translated into a significant yield increase, representing an additional 0.57 g IgG from each litre of plasma processed.
Example 4
[0103] 334 g Of B+I precipitate was re-suspended in 1,335 g of 17 vol. % ethanol buffer and mixed for 2 hours at a temperature of −5° C. The liquid phase (“first extract”) was then separated from the solid phase by centrifugation.
[0104] 274 g of the solid phase precipitate was re-suspended in 1,096 g of 17 vol. % ethanol buffer and mixed for 2 hours at a temperature of −5° C. The liquid phase (“second extract”) was then separated from the solid phase by centrifugation.
[0105] The pre-centrifuged suspension and the supernatant from each procedure were analysed for the presence of IgG and several other proteins of interest (Table 5). This confirmed that repeated sequential extractions of B+I precipitate yielded IgG in the solvent supernatant phase, with equivalent reduction in other proteins.
TABLE-US-00005 TABLE 5 First Extract Second Extract Sample (supernatant) (supernatant) IgG (mg/mL) 4.22 2.17 IgG (g per L plasma) 0.71 0.40 Albumin (mg/mL) 0.95 0.60 IgA (mg/mL) 0.26 0.091 IgM (mg/mL) 0.18 0.12 FXIa (ng/mL) 2.40 1.31
Example 5
[0106] Extract of B+I precipitate was prepared by mixing one part of precipitate with four parts of 17 vol. % ethanol buffer for two hours at a temperature of −5° C. The extract was then separated from the residual precipitate by centrifugation.
[0107] The extract of B+I precipitate was then combined with B+I supernatant in a ratio of 1:10 by volume. The composition of the B+I supernatant was compared with the composition of the combined B+I supernatant and B+I precipitate extract (Table 6). This confirmed that the supernatant could be combined with the precipitate extract to increase the IgG concentration while retaining an acceptable IgG purity profile.
TABLE-US-00006 TABLE 6 B + I Supernatant + B + I Sample B + I Supernatant precipitate extract IgG (mg/mL) 2.53 2.74 Albumin (mg/mL) 0.22 0.31 IgA (mg/mL) 0.02 0.04 IgM (mg/mL) <0.099 <0.099 FXIa (ng/mL) <0.6 <0.6 FVII (ng/mL) 4.65 6.75 FIX (ng/mL) 83.7 87.6 FXII (ng/mL) 260 603
Example 6
[0108] 250 kg Of B+I precipitate from plasma fractionated according to the modified Kistler and Nitschmann method was conditioned at 0° C.±2° C. and resuspended by homogenisation at 0° C.±2° C. for one hour in the presence of 1000 kg 17 vol. % ethanol buffer. The mixture was then matured at 0° C.±2° C. for 2 hours, after which the pH was 5.14 and the conductivity was 0.6 mS/cm. The supernatant extract was separated from the precipitate by filtration or by centrifugation. This extraction method and recovery by either filtration or centrifugation successfully extracted IgG of high purity from the B+I precipitate (Table 7).
TABLE-US-00007 TABLE 7 B + I Supernatant extract B + I Supernatant extract Sample recovered by filtration recovered by centrifugation IgG (g/L) 2.22 3.28 IgG (g per L 0.36 0.53 plasma Albumin (g/L) 0.41 0.85 IgA (g/L) <0.02 0.1 IgM (g/L) <0.10 <0.1 FXIa (ng/mL) NQ 2.16 Plasmin (U/mL) <0.1 <0.1 Plasminogen 0.15 0.3 (U/mL)
Example 7
[0109] 313 kg Of supernatant extract filtrate from Example 6 was adjusted to an ionic strength of 3.9 mS/cm, the ethanol concentration was raised to 25 vol. %, and the pH adjusted to pH 6.9 by titration with 1M sodium hydroxide and then incubated for 6 hours at −6.5° C. to yield 2.4 kg Fraction II precipitate which was collected by centrifugation. The Fraction II (Fr II) precipitate was dissolved in water (FrII:water=1:2). Analysis shows that the supernatant extract of B+I precipitate is compatible with further downstream purification to yield IgG with low levels of aggregate (Table 8).
TABLE-US-00008 TABLE 8 Composition of redissolved Fraction II precipitate from extracted B + I precipitate Redissolved FrII precipitate from Sample extracted B + I precipitate IG (g/L) 88.1 IgG (g per L plasma) 0.32 Albumin (g/L) 6.91 IgA (g/L) 0.59 IgM (g/L) 0.23 FXIa (ng/mL) 1.51 Plasmin (U/mL) <0.1 Plasminogen (U/mL) 0.16 % aggregate (by HPLC) 1.31
Example 8
[0110] 100 g Of B+I precipitate which had been stored frozen at less than −30° C. was brought to 0° C. without any agitation either in a vessel controlled to 0° C. (option (a)) or in a vessel containing 17 vol. % ethanol buffer which was controlled to 0° C. (option (b)). The temperature was measured by probes which had been placed in the precipitate before freezing. When the precipitate temperature reached 0° C.±1° C., 17 vol. % ethanol buffer was added to the precipitate from option (a) and both precipitates were homogenised prior to extraction of IgG into the solvent buffer with agitation for not less than 2 hours (maturation/conditioning). The supernatant was then separated from the remaining precipitate and analysed. There was equivalent IgG extraction from precipitate when conditioned to 0° C. with or without buffer (Table 9).
TABLE-US-00009 TABLE 9 Supernatant extract from B + I precipitate conditioned to 0° C. Sample Without buffer With buffer IgG (g/L) 5.21 5.40 IgG (g per L plasma) 0.87 0.94 Albumin (g/L) 1.51 1.73 IgA (g/L) 0.33 0.50 IgM (g/L) 0.32 0.44 FXIa (ng/mL) 8.14 11.6 Plasmin (U/mL) 0.22 0.19 Plasminogen (U/mL) 0.57 1.23