Methods and compositions for rapidly replacing cardiac myosin binding protein-C in sarcomeres

Abstract

Methods and compositions for rapidly replacing cMyBP-C in sarcomeres featuring the creation of Spy-C mice, which are mice genetically engineered to express cMyBP-C with a protease recognition site and SpyTag peptide introduced into the cMyBP-C gene. In permeabilized myocytes from the Spy-C mice, the cMyBP-C protein can be cleaved at the protease recognition site, and the N-Terminus of cMyBP-C can be removed while the C-terminus remains anchored to the thick filament. A new peptide featuring the SpyCatcher sequence can be covalently bonded to the remaining portion of cMyBP-C, thereby creating a modified cMyBP-C protein. The methods and compositions of the present invention allow for the reconstitution of full-length cMyBP-C at the precise position of native cMyBP-C in the sarcomere and allow for a variety of modifications to be introduced to cMyBP-C in situ.

Claims

1. A recombinant protein comprising cMyBP-C with an internal insertion therein, the internal insertion comprises at least a SpyTag peptide adjacent to a protease recognition site, wherein the internal insertion is within or between C7 and C8 domains of cMyBP-C.

2. A recombinant protein comprising cMyBP-C with an internal insertion therein, the internal insertion comprises at least a SpyTag peptide adjacent to a protease recognition site, wherein the internal insertion is in between residues VQEILQR (SEQ ID NO: 14) in domain C7 and residues PRLQLPRH (SEQ ID NO: 15) of domain C8 of cMyBP-C.

3. A recombinant protein comprising cMyBP-C with an internal insertion therein, wherein the recombinant cMyBP-C peptide is according to SEQ ID NO: 13.

4. A method of producing a recombinant cMyBP-C protein, said method comprising: a. introducing into a genome of a host a cassette for expressing a first recombinant cMyBP-C protein, wherein the first recombinant protein is according to the recombinant protein of claim 1, wherein the protease recognition site is adjacent to and 5′ to the SpyTag peptide; b. isolating myocytes from the host; c. introducing to the myocytes a protease that cleaves the first cMyBP-C protein at the protease recognition site to remove its N-terminus and expose the SpyTag peptide; d. introducing a recombinant cMyBP-C N-terminus, the recombinant cMyBP-C N-terminus being at least a portion of cMyBP-C with a SpyCatcher peptide at its C-terminus; wherein the SpyCatcher peptide of the cMyBP-C N-terminus binds to the SpyTag peptide of the first recombinant cMyBP-C to produce a second recombinant cMyBP-C protein.

5. A motility assay platform comprising: a coverslip with a thick filament disposed thereon, wherein the thick filament comprises a recombinant cMyBP-C protein according to claim 1.

6. A method of producing a recombinant cMyBP-C protein, said method comprising: a. introducing into a genome of a host a cassette for expressing a first recombinant cMyBP-C protein, wherein the first recombinant protein is according to the recombinant protein of claim 2, wherein the protease recognition site is adjacent to and 5′ to the SpyTag peptide; b. isolating myocytes from the host; c. introducing to the myocytes a protease that cleaves the first cMyBP-C protein at the protease recognition site to remove its N-terminus and expose the SpyTag peptide; d. introducing a recombinant cMyBP-C N-terminus, the recombinant cMyBP-C N-terminus being at least a portion of cMyBP-C with a SpyCatcher peptide at its C-terminus; wherein the SpyCatcher peptide of the cMyBP-C N-terminus binds to the SpyTag peptide of the first recombinant cMyBP-C to produce a second recombinant cMyBP-C protein.

7. A motility assay platform comprising: a coverslip with a thick filament disposed thereon, wherein the thick filament comprises a recombinant cMyBP-C protein according to claim 2.

8. A method of producing a recombinant cMyBP-C protein, said method comprising: a. introducing into a genome of a host a cassette for expressing a first recombinant cMyBP-C protein, the first recombinant cMyBP-C protein being a cMyBP-C peptide with an internal insertion therein, the internal insertion comprising a protease recognition site adjacent to and 5′ to a SpyTag peptide, wherein the internal insertion is within or between C7 and C8 domains of cMyBP-C; b. isolating myocytes from the host; c. introducing to the myocytes a protease that cleaves the first cMyBP-C protein at the protease recognition site to remove its N-terminus and expose the SpyTag peptide; d. introducing a recombinant cMyBP-C N-terminus, the recombinant cMyBP-C N-terminus being at least a portion of cMyBP-C with a SpyCatcher peptide at its C-terminus; wherein the SpyCatcher peptide of the cMyBP-C N-terminus binds to the SpyTag peptide of the first recombinant cMyBP-C to produce a second recombinant cMyBP-C protein according to claim 3.

9. A motility assay platform comprising: a coverslip with a thick filament disposed thereon, wherein the thick filament comprises a recombinant cMyBP-C protein according to claim 3.

Description

BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWING(S)

(1) The features and advantages of the present invention will become apparent from a consideration of the following detailed description presented in connection with the accompanying drawings in which:

(2) FIG. 1. Shows a schematic view of the structure of cMyBP-C as a series of 11 folded domains (purple circles) numbered C0-C10 along with the regulatory M-domain between C1 and C2. The M-domain contains 3 cardiac-specific phosphorylation sites known to regulate effects of cMyBP-C in response to inotropic agonists, but 14 additional phosphorylation sites have been identified whose function is unknown. The N′-terminal domains C0-C2 contribute to the regulatory effects of cMyBP-C on contraction in part by binding to the thin filament in a phosphorylation dependent manner. Domains C8-C10 anchor cMyBP-C to the thick filament.

(3) FIG. 2 shows a schematic view of the method of the present invention, e.g., the rapid exchange of cMyBP-C in Spy-C permeabilized myocytes. Spy-C mice express cMyBP-C with a TEV protease recognition site (blue rectangle) and a SpyTag sequence (orange rectangle) inserted between domains C7 and C8. (1) TEV cleavage of Spy-C cMyBP-C in permeabilized myocytes first removes domains C0-C7, which (2) are subsequently replaced by new recombinant C0-C7 domains (purple) containing desired phosphorylation site mutations (example sites in C0, M, and C4 indicated by red asterisks). The new C0-C7 domains covalently attach to C8-C10 via the SpyCatcher and SpyTag bond. Because domains C8-C10 remain anchored to the thick filament after TEV cleavage, the Spy-C method efficiently achieves replacement of cMyBP-C in situ.

(4) FIG. 3 shows force measurements in cardiomyocytes from wild type (WT) and homozygous (HO) Spy-C mice, demonstrating overtly normal force generation in cardiomyocytes from Spy-C mice. Top left panel shows normal tension-pCa relationships in WT and HO myocytes, while the top right panel shows summary data illustrating that the midpoints of the tension-pCa relationships are not different. Bottom panels show that maximal force (F.sub.max) and passive tension (F.sub.pas) also are not different between WT and HO myocytes.

(5) FIG. 4 shows a typical in vitro motility assay (prior art).

(6) FIG. 5 shows a motility assay using thick filaments, highlighting where cMyBP-C proteins (e.g., recombinant cMyBP-C proteins made using the methods and compositions herein) are located (image source: Previs et al., Science 2012, 337:1215-1218). Myosin molecules are maintained in their native filamentous state (blue). Full-length cMyBP-C (orange) can be retained at its proper locations on thick filaments.

TERMS

(7) Unless otherwise explained, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which a disclosed invention belongs. The singular terms “a,” “an,” and “the” include plural referents unless context clearly indicates otherwise. Similarly, the word “or” is intended to include “and” unless the context clearly indicates otherwise. The term “comprising” means that other elements can also be present in addition to the defined elements presented. The use of “comprising” indicates inclusion rather than limitation. Stated another way, the term “comprising” means “including principally, but not necessary solely”. Furthermore, variation of the word “comprising”, such as “comprise” and “comprises”, have correspondingly the same meanings. In one respect, the technology described herein related to the herein described compositions, methods, and respective component(s) thereof, as essential to the invention, yet open to the inclusion of unspecified elements, essential or not (“comprising”).

(8) All embodiments disclosed herein can be combined with other embodiments unless the context clearly dictates otherwise.

(9) Suitable methods and materials for the practice and/or testing of embodiments of the disclosure are described below. Such methods and materials are illustrative only and are not intended to be limiting. Other methods and materials similar or equivalent to those described herein can be used. For example, conventional methods well known in the art to which the disclosure pertains are described in various general and more specific references, including, for example, Sambrook et al., Molecular Cloning: A Laboratory Manual, 2d ed., Cold Spring Harbor Laboratory Press, 1989; Sambrook et al., Molecular Cloning: A Laboratory Manual, 3d ed., Cold Spring Harbor Press, 2001; Ausubel et al., Current Protocols in Molecular Biology, Greene Publishing Associates, 1992 (and Supplements to 2000); Ausubel et al., Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology, 4th ed., Wiley & Sons, 1999; Harlow and Lane, Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, 1990; and Harlow and Lane, Using Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory. Press, 1999, Gene Expression Technology (Methods in Enzymology, Vol. 185, edited by D. Goeddel, 1991. Academic Press, San Diego, Calif.), “Guide to Protein Purification” in Methods in Enzymology (M. P. Deutshcer, ed., (1990) Academic Press, Inc.); PCR Protocols: A Guide to Methods and Applications (Innis, et al. 1990. Academic Press, San Diego, Calif.), Culture of Animal Cells: A Manual of Basic Technique, 2.sup.nd Ed. (R. I. Freshney. 1987. Liss, Inc. New York, N.Y.), Gene Transfer and Expression Protocols, pp. 109-128, ed. E. J. Murray, The Humana Press Inc., Clifton, N.J.), and the Ambion 1998 Catalog (Ambion, Austin, Tex.), the disclosures of which are incorporated in their entirety herein by reference.

(10) All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety for all purposes. In case of conflict, the present specification, including explanations of terms, will control.

(11) Although methods and materials similar or equivalent to those described herein can be used to practice or test the disclosed technology, suitable methods and materials are described below. The materials, methods, and examples are illustrative only and not intended to be limiting.

(12) In order to facilitate review of the various embodiments of the disclosure, the following explanations of specific terms are provided:

(13) CRISPR/Cas9: A genome editing tool that enables the editing of parts of the genome by removing, adding, or altering sections of the DNA sequence. Cas9 is an enzyme that acts as a pair of molecular scissors to cut the two stands of DNA at a specific location in the genome so that other DNA can be added or removed. Guide RNA (gRNA) is a small piece of pre-designed RNA located within a longer RNA scaffold. The scaffold part binds to DNA and the pre-designed sequence guides Cas9 to the right part of the genome. The gRNA has a sequence that is complementary to the target DNA sequence. After the DNA is cut, the cell recognizes the DNA damage and tries to repair it. The DNA repair machinery can be used to introduce changes to the gene.

(14) Modification: Changes to a protein sequence, structure, etc., or changes to a nucleic acid sequence, etc. As used herein, the term “modified” or “modification,” can include one or more mutations, deletions, substitutions, physical alteration (e.g., cross-linking modification, covalent bonding of a component, post-translational modification, e.g., acetylation, glycosylation, the like, or a combination thereof), the like, or a combination thereof. Modification, e.g., mutation, is not limited to random modification (e.g., random mutagenesis) but includes rational design as well.

(15) Recombinant nucleic acid: A nucleic acid having nucleotide sequences that are not naturally joined together and can be made by artificially combining two otherwise separated segments of sequence. This artificial combination is often accomplished by chemical synthesis or, more commonly, by the artificial manipulation of isolated segments of nucleic acids, for example, by genetic engineering techniques. Recombinant nucleic acids include nucleic acid vectors comprising an amplified or assembled nucleic acid, which can be used to transform or transfect a suitable host cell. A host cell that comprises the recombinant nucleic acid is referred to as a “recombinant host cell.” The gene is then expressed in the recombinant host cell to produce a “recombinant polypeptide.” A recombinant nucleic acid can also serve a non-coding function (for example, promoter, origin of replication, ribosome-binding site and the like).

(16) Recombinant protein: For a recombinant nucleic acid, see “Recombinant Nucleic Acid” above. A recombinant protein or polypeptide is one that has a sequence that is not naturally occurring or has a sequence that is made by an artificial combination of two otherwise separated segments of sequence. This artificial combination is often accomplished by chemical synthesis or, more commonly, by the artificial manipulation of isolated segments of nucleic acids, for example, by genetic engineering techniques. Recombinant proteins may be made in cells transduced, transfected, or transformed with genetic elements to direct the synthesis of the heterologous protein. They may also be made in cell-free systems.

DETAILED DESCRIPTION OF THE INVENTION

(17) The present invention features methods and compositions for rapidly replacing cMyBP-C in its normal position in sarcomeres. The methods herein feature the use of a gene-edited mouse model platform that allows for rapid exchange of new combinations of modified or mutant cMyBP-C, such as phosphorylation site mutants, insertions, deletions, fluorescent probes, etc.

(18) Split Peptide Pairs

(19) The present invention features the use of a split peptide pair, e.g., a pair of peptides derived from the splitting of a protein into two halves, each with reactive residues. The split peptide pair is engineered to recombine and form covalent bonds (irreversible isopeptide linkages), thereby creating a new fusion protein. A non-limiting example of a split peptide pair includes SpyCatcher/SpyTag. SpyCatcher and SpyTag are well known to one of ordinary skill in the art. SpyTag and SpyCatcher were generating by splitting the CnaB2 domain from the fibronectin-binding protein FbaB from Streptococcus pyogenes (Spy). CnaB2 was split into a 13 residue peptide (SpyTag) and the 116 residue complementary domain (SpyCatcher). These two parts spontaneously reconstitute to form an isopeptide bond under a range of temperatures, pH values, buffers, and even with non-ionic detergents. SpyTag and SpyCatcher function well when fused at either the N-terminus or C-terminus (see Zakeri et al., PNAS 2012, 109:E690-697; Reddington and Howarth, Current Opinion in Chemical Biology 2015, 29:94-99. SpyCatcher/SpyTag has been used previously to create genetic fusions including several platform technologies such as synthetic vaccine production and catalytic circulation.

(20) As used in the present invention. The SpyCatcher and SpyTag pair form a spontaneous isopeptide bond between a reactive lysine of SpyCatcher and a reactive aspartate of SpyTag when each half of the pair is expressed as a fusion protein with cMyBP-C. The present invention is not limited to SpyCatcher/SpyTag. Any appropriate split peptide pair that is currently available or that may be developed in the future may be contemplated. For example, in certain embodiments, the split peptide pair is SnoopCatcher/SnoopTag.

(21) Spy-C Method

(22) In the present invention, the SpyCatcher/SpyTag technology was applied to generically engineered mice (see FIG. 2). Spy-C mice were created using CRISPR/Cas9 gene editing to introduce a protease recognition site (e.g., tobacco etch virus (TEV) protease recognition site, blue rectangle in FIG. 2) and a SpyTag sequence (orange rectangle in FIG. 2) in the linker between domains C7 and C8 of cMyBP-C. The protease recognition site allows a portion (e.g., the N-terminus) of cMyBP-C to be cut and removed, and the SpyTag sequence allows another peptide with a SpyCatcher sequence to be attached to the remaining portion of cMyBP-C. Note that TEV is highly specific for a consensus sequence. The sequence is rare in mammalian proteins.

(23) To achieve rapid exchange of cMyBP-C in permeabilized myocytes, Spy-C cMyBP-C was cleaved at the TEV site. Domains C0-C7 of Spy-C cMyBP-C were then readily washed out and replaced with novel recombinant cMyBP-C-SpyCatcher containing desired phosphorylation site mutations by adding proteins directly to the bath surrounding the permeabilized myocytes. Added C0-C7-SpyCatcher (purple rectangle in FIG. 2) formed a permanent covalent bond with SpyTag (orange rectangle in FIG. 2) encoded at the beginning of domains C8-C10. Domains C8-C10 remained anchored to the thick filament because these domains contain the major binding sites for myosin and titin and densities attributable to domains C8-C10 are visible in EM reconstructions of isolated thick filaments. Note that other insertion sites may be possible.

(24) By fusing the SpyCatcher sequence to new recombinant cMyBP-C N-terminal sequences, it is possible to reconstitute full-length cMyBP-C at the precise position of native cMyBP-C in the sarcomere. Thus, the present invention provides methods for efficiently cutting and pasting cMyBP-C in situ to introduce virtually any desired modification (e.g., point mutations, FRET probes, etc.) to study cMyBP-C interactions with its binding partners in sarcomeres or to provide recombinant cMyBP-C peptides for various purposes.

(25) It was surprisingly found that the Spy-C mice generated were phenotypically normal. Given the importance of the cMyBP-C protein, it was expected that disruption of the cMyBP-C protein would have deleterious effects that might preclude the usefulness of the mouse model. However, echocardiography demonstrated the Spy-C mice had no overt cardiac phenotype up to 12 months of age in founders or progeny. Further, cMyBP-C expression was normal and cMyBP-C was properly localized in sarcomeres in Spy-C mice. FIG. 3 shows force measurements in wild type and Spy-C mice, demonstrating overtly normal force generation in cardiomyocytes from Spy-C mice. Inventors were also surprised that the protein was not destabilized by the insertion of the TEV protease site and SpyTag between the C7-C8 regions. There is not much known about the C-terminal end of the cMyBP-C protein, so it was not clear if the insertion would work, or where the insertion should be placed.

(26) Non-limiting examples of sequences encoding modified cMyBP-C proteins are included in Table 1. SEQ ID NO: 1 is a cDNA sequence for wild-type cMyBP-C C0-C7 domains with SpyCatcher followed by a TEV recognition site, His Tag, and Stop codon. SEQ ID NO: 1 is used to make C0-C7-sc replacement domains after TEV cleavage of cMyBP-C in myocytes from Spy-C mice. SEQ: ID NO: 2 is a cDNA sequence for cMyBP-C C0-C7 domains containing the [E330K] mutation with SpyCatcher followed by a TEV recognition site, His Tag, and Stop codon. SEQ: ID NO: 2 is used to make C0-C7-sc replacement proteins with the specified mutation after TEV cleavage of cMyBP-C in myocytes from Spy-C mice. SEQ ID NO: 3 is a cDNA sequence for cMyBP-C C0-C7 domains containing the [L348P] mutation with SpyCatcher followed by a TEV recognition site, His Tag, and Stop codon. SEQ ID NO: 3 is used to make C0-C7-sc replacement proteins with the specified mutation after TEV cleavage of cMyBP-C in myocytes from Spy-C mice.

(27) TABLE-US-00001 TABLE 1 Description Sequence SEQ ID  ATGCCGGAGCCGGGTAAAAAGCCGGTTAGCGCGTTCAACAAAAAACCGCGTAGCGCGGAAG NO: 1 TGACCGCGGGTAGCGCGGCGGTGTTTGAGGCGGAAACCGAGCGTAGCGGCGTTAAAGTGC GTTGGCAACGTGACGGTAGCGATATTACCGCGAACGATAAATACGGTCTGGCGGCGGAAGG CAAGCGTCACACCCTGACCGTTCGTGATGCGAGCCCGGACGATCAGGGTAGCTATGCGGTG ATCGCGGGCAGCAGCAAGGTGAAATTTGACCTGAAAGTTACCGAGCCGGCTCCGCCGGAAA AAGCGGAAAGCGAGGTGGCGCCGGGTGCGCCGAAGGAAGTTCCGGCGCCGGCGACCGAG CTGGAGGAAAGCGTGAGCAGCCCGGAAGGCAGCGTTAGCGTGACCCAGGATGGTAGCGCG GCGGAACATCAAGGTGCGCCGGATGATCCGATCGGTCTGTTCCTGATGCGTCCGCAAGACG GTGAGGTTACCGTGGGTGGCAGCATTGTGTTTAGCGCGCGTGTTGCGGGTGCGAGCCTGCT GAAACCGCCGGTGGTTAAGTGGTTCAAGGGCAAATGGGTGGATCTGAGCAGCAAAGTTGGT CAGCACCTGCAACTGCACGACAGCTACGATCGTGCGAGCAAGGTTTACCTGTTCGAACTGCA CATTACCGATGCGCAGACCACCAGCGCGGGTGGCTACCGTTGCGAGGTTAGCACCAAGGAC AAATTCGATAGCTGCAACTTTAACCTGACCGTGCACGAAGCGATCGGTAGCGGCGACCTGG ATCTGCGTAGCGCGTTTCGTCGTACCAGCCTGGCGGGTGCGGGTCGTCGTACCAGCGACAG CCATGAGGATGCGGGCACCCTGGATTTCAGCAGCCTGCTGAAGAAACGTGATAGCTTTCGT CGTGACAGCAAACTGGAAGCGCCGGCGGAGGAAGACGTTTGGGAGATCCTGCGTCAAGCT CCGCCGAGCGAATACGAGCGTATTGCGTTCCAGCACGGTGTGACCGATCTGCGTGGCATGC TGAAGCGTCTGAAGGGTATGAAGCAGGACGAAAAGAAAAGCACCGCGTTTCAGAAGAAACT GGAGCCGGCGTATCAAGTGAACAAAGGCCACAAGATCCGTCTGACCGTGGAACTGGCGGAC CCGGATGCGGAAGTGAAATGGCTGAAGAACGGCCAGGAAATCCAAATGAGCGGTAGCAAAT ACATTTTCGAGAGCGTTGGTGCGAAGCGTACCCTGACCATTAGCCAATGCAGCCTGGCGGA CGATGCGGCGTATCAGTGCGTGGTTGGTGGCGAGAAATGCAGCACCGAACTGTTCGTGAAG GAGCCGCCGGTTCTGATCACCCGTAGCCTGGAAGATCAGCTGGTTATGGTGGGTCAACGTG TGGAATTTGAGTGCGAAGTTAGCGAGGAAGGCGCGCAAGTGAAATGGCTGAAGGACGGTGT TGAGCTGACCCGTGAGGAAACCTTCAAATACCGTTTTAAGAAAGATGGTCGTAAGCACCACC TGATCATTAACGAAGCGACCCTGGAGGATGCGGGTCACTATGCGGTTCGTACCAGCGGTGG CCAGAGCCTGGCGGAACTGATCGTGCAAGAAAAGAAACTGGAAGTGTATCAGAGCATTGCG GATCTGGCGGTGGGTGCGAAAGACCAGGCGGTGTTCAAGTGCGAAGTTAGCGATGAGAACG TTCGTGGTGTGTGGCTGAAAAACGGCAAGGAGCTGGTTCCGGACAACCGTATCAAAGTGAG CCACATTGGTCGTGTTCACAAGCTGACCATCGACGATGTTACCCCGGCGGACGAAGCGGAT TATAGCTTCGTGCCGGAGGGCTTTGCGTGCAACCTGAGCGCGAAACTGCACTTCATGGAAG TGAAGATCGACTTTGTTCCGCGTCAGGAGCCGCCGAAAATTCATCTGGATTGCCCGGGTAGC ACCCCGGACACCATTGTGGTTGTGGCGGGTAACAAACTGCGTCTGGATGTGCCGATTAGCG GCGACCCGGCGCCGACCGTTGTGTGGCAGAAGACCGTGACCCAAGGTAAGAAAGCGAGCA CCGGTCCGCACCCGGATGCGCCGGAGGATGCGGGTGCGGACGAGGAATGGGTTTTCGATA AGAAACTGCTGTGCGAAACCGAAGGCCGTGTTCGTGTGGAAACCACCAAGGATCGTAGCGT TTTTACCGTGGAGGGCGCGGAGAAAGAAGACGAGGGTGTTTACACCGTTACCGTGAAGAAC CCGGTGGGTGAAGACCAGGTTAACCTGACCGTTAAAGTTATTGATGTTCCGGATGCGCCGG CGGCGCCGAAGATTAGCAACGTGGGTGAAGATAGCTGCACCGTTCAATGGGAGCCGCCGG CGTATGATGGTGGCCAGCCGGTGCTGGGCTATATCCTGGAGCGTAAGAAAAAGAAAAGCTA TCGTTGGATGCGTCTGAACTTCGACCTGCTGCGTGAACTGAGCCACGAGGCGCGTCGTATG ATTGAAGGTGTTGCGTACGAGATGCGTGTTTATGCGGTGAACGCGGTTGGTATGAGCCGTC CGAGCCCGGCGAGCCAGCCGTTTATGCCGATTGGTCCGCCGGGTGAACCGACCCACCTGG CGGTGGAGGACGTTAGCGATACCACCGTGAGCCTGAAATGGCGTCCGCCGGAACGTGTTG GTGCGGGTGGCCTGGATGGCTACAGCGTGGAATATTGCCAAGAGGGCTGCAGCGAATGGA CCCCGGCGCTGCAGGGTCTGACCGAGCGTACCAGCATGCTGGTTAAAGACCTGCCGACCG GTGCGCGTCTGCTGTTCCGTGTGCGTGCGCATAACGTTGCGGGTCCGGGTGGCCCGATCGT GACCAAGGAACCGGTTACCGTGCAGGAGATT[AGCGGTGATAGCGCGACCCACATTAAATTT AGCAAGCGTGACGAGGATGGTAAAGAACTGGCGGGCGCGACCATGGAACTGCGTGACAGC AGCGGCAAGACCATCAGCACCTGGATTAGCGATGGTCAGGTTAAAGACTTCTACCTGTATCC GGGCAAGTACACCTTTGTGGAAACCGCGGCGCCGGATGGTTATGAGGTGGCGACCGCGAT CACCTTCACCGTTAACGAGCAGGGTCAAGTTACCGTGAACGGTAAAGCGACCAAGGGC][GA GAATCTGTATTTCCAGGGT][CATCACCACCACCATCAC]TAA SEQ ID  ATGCCGGAGCCGGGTAAAAAGCCGGTTAGCGCGTTCAACAAAAAACCGCGTAGCGCGGAAG NO: 2 TGACCGCGGGTAGCGCGGCGGTGTTTGAGGCGGAAACCGAGCGTAGCGGCGTTAAAGTGC GTTGGCAACGTGACGGTAGCGATATTACCGCGAACGATAAATACGGTCTGGCGGCGGAAGG CAAGCGTCACACCCTGACCGTTCGTGATGCGAGCCCGGACGATCAGGGTAGCTATGCGGTG ATCGCGGGCAGCAGCAAGGTGAAATTTGACCTGAAAGTTACCGAGCCGGCTCCGCCGGAAA AAGCGGAAAGCGAGGTGGCGCCGGGTGCGCCGAAGGAAGTTCCGGCGCCGGCGACCGAG CTGGAGGAAAGCGTGAGCAGCCCGGAAGGCAGCGTTAGCGTGACCCAGGATGGTAGCGCG GCGGAACATCAAGGTGCGCCGGATGATCCGATCGGTCTGTTCCTGATGCGTCCGCAAGACG GTGAGGTTACCGTGGGTGGCAGCATTGTGTTTAGCGCGCGTGTTGCGGGTGCGAGCCTGCT GAAACCGCCGGTGGTTAAGTGGTTCAAGGGCAAATGGGTGGATCTGAGCAGCAAAGTTGGT CAGCACCTGCAACTGCACGACAGCTACGATCGTGCGAGCAAGGTTTACCTGTTCGAACTGCA CATTACCGATGCGCAGACCACCAGCGCGGGTGGCTACCGTTGCGAGGTTAGCACCAAGGAC AAATTCGATAGCTGCAACTTTAACCTGACCGTGCACGAAGCGATCGGTAGCGGCGACCTGG ATCTGCGTAGCGCGTTTCGTCGTACCAGCCTGGCGGGTGCGGGTCGTCGTACCAGCGACAG CCATGAGGATGCGGGCACCCTGGATTTCAGCAGCCTGCTGAAGAAACGTGATAGCTTTCGT CGTGACAGCAAACTGGAAGCGCCGGCGGAGGAAGACGTTTGGGAGATCCTGCGTCAAGCT CCGCC[AAG]CGAATACGAGCGTATTGCGTTCCAGCACGGTGTGACCGATCTGCGTGGCATG CTGAAGCGTCTGAAGGGTATGAAGCAGGACGAAAAGAAAAGCACCGCGTTTCAGAAGAAAC TGGAGCCGGCGTATCAAGTGAACAAAGGCCACAAGATCCGTCTGACCGTGGAACTGGCGGA CCCGGATGCGGAAGTGAAATGGCTGAAGAACGGCCAGGAAATCCAAATGAGCGGTAGCAAA TACATTTTCGAGAGCGTTGGTGCGAAGCGTACCCTGACCATTAGCCAATGCAGCCTGGCGG ACGATGCGGCGTATCAGTGCGTGGTTGGTGGCGAGAAATGCAGCACCGAACTGTTCGTGAA GGAGCCGCCGGTTCTGATCACCCGTAGCCTGGAAGATCAGCTGGTTATGGTGGGTCAACGT GTGGAATTTGAGTGCGAAGTTAGCGAGGAAGGCGCGCAAGTGAAATGGCTGAAGGACGGTG TTGAGCTGACCCGTGAGGAAACCTTCAAATACCGTTTTAAGAAAGATGGTCGTAAGCACCAC CTGATCATTAACGAAGCGACCCTGGAGGATGCGGGTCACTATGCGGTTCGTACCAGCGGTG GCCAGAGCCTGGCGGAACTGATCGTGCAAGAAAAGAAACTGGAAGTGTATCAGAGCATTGC GGATCTGGCGGTGGGTGCGAAAGACCAGGCGGTGTTCAAGTGCGAAGTTAGCGATGAGAAC GTTCGTGGTGTGTGGCTGAAAAACGGCAAGGAGCTGGTTCCGGACAACCGTATCAAAGTGA GCCACATTGGTCGTGTTCACAAGCTGACCATCGACGATGTTACCCCGGCGGACGAAGCGGA TTATAGCTTCGTGCCGGAGGGCTTTGCGTGCAACCTGAGCGCGAAACTGCACTTCATGGAA GTGAAGATCGACTTTGTTCCGCGTCAGGAGCCGCCGAAAATTCATCTGGATTGCCCGGGTA GCACCCCGGACACCATTGTGGTTGTGGCGGGTAACAAACTGCGTCTGGATGTGCCGATTAG CGGCGACCCGGCGCCGACCGTTGTGTGGCAGAAGACCGTGACCCAAGGTAAGAAAGCGAG CACCGGTCCGCACCCGGATGCGCCGGAGGATGCGGGTGCGGACGAGGAATGGGTTTTCGA TAAGAAACTGCTGTGCGAAACCGAAGGCCGTGTTCGTGTGGAAACCACCAAGGATCGTAGC GTTTTTACCGTGGAGGGCGCGGAGAAAGAAGACGAGGGTGTTTACACCGTTACCGTGAAGA ACCCGGTGGGTGAAGACCAGGTTAACCTGACCGTTAAAGTTATTGATGTTCCGGATGCGCCG GCGGCGCCGAAGATTAGCAACGTGGGTGAAGATAGCTGCACCGTTCAATGGGAGCCGCCG GCGTATGATGGTGGCCAGCCGGTGCTGGGCTATATCCTGGAGCGTAAGAAAAAGAAAAGCT ATCGTTGGATGCGTCTGAACTTCGACCTGCTGCGTGAACTGAGCCACGAGGCGCGTCGTAT GATTGAAGGTGTTGCGTACGAGATGCGTGTTTATGCGGTGAACGCGGTTGGTATGAGCCGT CCGAGCCCGGCGAGCCAGCCGTTTATGCCGATTGGTCCGCCGGGTGAACCGACCCACCTG GCGGTGGAGGACGTTAGCGATACCACCGTGAGCCTGAAATGGCGTCCGCCGGAACGTGTT GGTGCGGGTGGCCTGGATGGCTACAGCGTGGAATATTGCCAAGAGGGCTGCAGCGAATGG ACCCCGGCGCTGCAGGGTCTGACCGAGCGTACCAGCATGCTGGTTAAAGACCTGCCGACC GGTGCGCGTCTGCTGTTCCGTGTGCGTGCGCATAACGTTGCGGGTCCGGGTGGCCCGATC GTGACCAAGGAACCGGTTACCGTGCAGGAGATT[AGCGGTGATAGCGCGACCCACATTAAAT TTAGCAAGCGTGACGAGGATGGTAAAGAACTGGCGGGCGCGACCATGGAACTGCGTGACAG CAGCGGCAAGACCATCAGCACCTGGATTAGCGATGGTCAGGTTAAAGACTTCTACCTGTATC CGGGCAAGTACACCTTTGTGGAAACCGCGGCGCCGGATGGTTATGAGGTGGCGACCGCGA TCACCTTCACCGTTAACGAGCAGGGTCAAGTTACCGTGAACGGTAAAGCGACCAAGGGC][G AGAATCTGTATTTCCAGGGT][CATCACCACCACCATCAC]TAA SEQ ID  ATGCCGGAGCCGGGTAAAAAGCCGGTTAGCGCGTTCAACAAAAAACCGCGTAGCGCGGAAG NO: 3 TGACCGCGGGTAGCGCGGCGGTGTTTGAGGCGGAAACCGAGCGTAGCGGCGTTAAAGTGC GTTGGCAACGTGACGGTAGCGATATTACCGCGAACGATAAATACGGTCTGGCGGCGGAAGG CAAGCGTCACACCCTGACCGTTCGTGATGCGAGCCCGGACGATCAGGGTAGCTATGCGGTG ATCGCGGGCAGCAGCAAGGTGAAATTTGACCTGAAAGTTACCGAGCCGGCTCCGCCGGAAA AAGCGGAAAGCGAGGTGGCGCCGGGTGCGCCGAAGGAAGTTCCGGCGCCGGCGACCGAG CTGGAGGAAAGCGTGAGCAGCCCGGAAGGCAGCGTTAGCGTGACCCAGGATGGTAGCGCG GCGGAACATCAAGGTGCGCCGGATGATCCGATCGGTCTGTTCCTGATGCGTCCGCAAGACG GTGAGGTTACCGTGGGTGGCAGCATTGTGTTTAGCGCGCGTGTTGCGGGTGCGAGCCTGCT GAAACCGCCGGTGGTTAAGTGGTTCAAGGGCAAATGGGTGGATCTGAGCAGCAAAGTTGGT CAGCACCTGCAACTGCACGACAGCTACGATCGTGCGAGCAAGGTTTACCTGTTCGAACTGCA CATTACCGATGCGCAGACCACCAGCGCGGGTGGCTACCGTTGCGAGGTTAGCACCAAGGAC AAATTCGATAGCTGCAACTTTAACCTGACCGTGCACGAAGCGATCGGTAGCGGCGACCTGG ATCTGCGTAGCGCGTTTCGTCGTACCAGCCTGGCGGGTGCGGGTCGTCGTACCAGCGACAG CCATGAGGATGCGGGCACCCTGGATTTCAGCAGCCTGCTGAAGAAACGTGATAGCTTTCGT CGTGACAGCAAACTGGAAGCGCCGGCGGAGGAAGACGTTTGGGAGATCCTGCGTCAAGCT CCGCCGAGCGAATACGAGCGTATTGCGTTCCAGCACGGTGTGACCGATCTGCGTGGCATGC TGAAGCGT[CCG]AAGGGTATGAAGCAGGACGAAAAGAAAAGCACCGCGTTTCAGAAGAAACT GGAGCCGGCGTATCAAGTGAACAAAGGCCACAAGATCCGTCTGACCGTGGAACTGGCGGAC CCGGATGCGGAAGTGAAATGGCTGAAGAACGGCCAGGAAATCCAAATGAGCGGTAGCAAAT ACATTTTCGAGAGCGTTGGTGCGAAGCGTACCCTGACCATTAGCCAATGCAGCCTGGCGGA CGATGCGGCGTATCAGTGCGTGGTTGGTGGCGAGAAATGCAGCACCGAACTGTTCGTGAAG GAGCCGCCGGTTCTGATCACCCGTAGCCTGGAAGATCAGCTGGTTATGGTGGGTCAACGTG TGGAATTTGAGTGCGAAGTTAGCGAGGAAGGCGCGCAAGTGAAATGGCTGAAGGACGGTGT TGAGCTGACCCGTGAGGAAACCTTCAAATACCGTTTTAAGAAAGATGGTCGTAAGCACCACC TGATCATTAACGAAGCGACCCTGGAGGATGCGGGTCACTATGCGGTTCGTACCAGCGGTGG CCAGAGCCTGGCGGAACTGATCGTGCAAGAAAAGAAACTGGAAGTGTATCAGAGCATTGCG GATCTGGCGGTGGGTGCGAAAGACCAGGCGGTGTTCAAGTGCGAAGTTAGCGATGAGAACG TTCGTGGTGTGTGGCTGAAAAACGGCAAGGAGCTGGTTCCGGACAACCGTATCAAAGTGAG CCACATTGGTCGTGTTCACAAGCTGACCATCGACGATGTTACCCCGGCGGACGAAGCGGAT TATAGCTTCGTGCCGGAGGGCTTTGCGTGCAACCTGAGCGCGAAACTGCACTTCATGGAAG TGAAGATCGACTTTGTTCCGCGTCAGGAGCCGCCGAAAATTCATCTGGATTGCCCGGGTAGC ACCCCGGACACCATTGTGGTTGTGGCGGGTAACAAACTGCGTCTGGATGTGCCGATTAGCG GCGACCCGGCGCCGACCGTTGTGTGGCAGAAGACCGTGACCCAAGGTAAGAAAGCGAGCA CCGGTCCGCACCCGGATGCGCCGGAGGATGCGGGTGCGGACGAGGAATGGGTTTTCGATA AGAAACTGCTGTGCGAAACCGAAGGCCGTGTTCGTGTGGAAACCACCAAGGATCGTAGCGT TTTTACCGTGGAGGGCGCGGAGAAAGAAGACGAGGGTGTTTACACCGTTACCGTGAAGAAC CCGGTGGGTGAAGACCAGGTTAACCTGACCGTTAAAGTTATTGATGTTCCGGATGCGCCGG CGGCGCCGAAGATTAGCAACGTGGGTGAAGATAGCTGCACCGTTCAATGGGAGCCGCCGG CGTATGATGGTGGCCAGCCGGTGCTGGGCTATATCCTGGAGCGTAAGAAAAAGAAAAGCTA TCGTTGGATGCGTCTGAACTTCGACCTGCTGCGTGAACTGAGCCACGAGGCGCGTCGTATG ATTGAAGGTGTTGCGTACGAGATGCGTGTTTATGCGGTGAACGCGGTTGGTATGAGCCGTC CGAGCCCGGCGAGCCAGCCGTTTATGCCGATTGGTCCGCCGGGTGAACCGACCCACCTGG CGGTGGAGGACGTTAGCGATACCACCGTGAGCCTGAAATGGCGTCCGCCGGAACGTGTTG GTGCGGGTGGCCTGGATGGCTACAGCGTGGAATATTGCCAAGAGGGCTGCAGCGAATGGA CCCCGGCGCTGCAGGGTCTGACCGAGCGTACCAGCATGCTGGTTAAAGACCTGCCGACCG GTGCGCGTCTGCTGTTCCGTGTGCGTGCGCATAACGTTGCGGGTCCGGGTGGCCCGATCGT GACCAAGGAACCGGTTACCGTGCAGGAGATT[AGCGGTGATAGCGCGACCCACATTAAATTT AGCAAGCGTGACGAGGATGGTAAAGAACTGGCGGGCGCGACCATGGAACTGCGTGACAGC AGCGGCAAGACCATCAGCACCTGGATTAGCGATGGTCAGGTTAAAGACTTCTACCTGTATCC GGGCAAGTACACCTTTGTGGAAACCGCGGCGCCGGATGGTTATGAGGTGGCGACCGCGAT CACCTTCACCGTTAACGAGCAGGGTCAAGTTACCGTGAACGGTAAAGCGACCAAGGGC][GA GAATCTGTATTTCCAGGGT][CATCACCACCACCATCAC]TAA

(28) Table 4 shows sequences for TEV protease consensus recognition site, SpyCatcher, and cMyBP-C.

(29) TABLE-US-00002 TABLE 4 Description Sequence SEQ ID NO: 4 GAGAATCTGTATTTCCAGGGT (Example of TEV Protease recognition  site) SEQ ID NO: 5 E-Xaa-Xaa-Y-Xaa-Q-G (TEV Protease recognition  site) SEQ ID NO: 6 E-Xaa-Xaa-Y-Xaa-Q-S (TEV Protease recognition  site) SEQ ID NO: 7 AGCGGTGATAGCGCGACCCACATTAAATTTAGCAAGCGTGACGAGGATGGTAA (SpyCatcher) AGAACTGGCGGGCGCGACCATGGAACTGCGTGACAGCAGCGGCAAGACCATC AGCACCTGGATTAGCGATGGTCAGGTTAAAGACTTCTACCTGTATCCGGGCAA GTACACCTTTGTGGAAACCGCGGCGCCGGATGGTTATGAGGTGGCGACCGCG ATCACCTTCACCGTTAACGAGCAGGGTCAAGTTACCGTGAACGGTAAAGCGAC CAAGGGC SEQ ID NO: 8 SGDSATHIKFSKRDEDGKELAGATMELRDSSGKTISTWISDGQVKDFYLYPGKYTF (SpyCatcher VETAAPDGYEVATAITFTVNEQGQVTVNGKATKG protein) SEQ ID NO: 9 AHIVMVDAYKPTK (SpyTag protein) SEQ ID NO: 10 MPEPGKKPVSAFNKKPRSAEVTAGSAAVFEAETERSGVMVRWQRDGSDITANDK (cMyBP-C YGLAAEGKRHTLTVRDASPDDQGSYAVIAGSSKVKFDLKVTEPAPPEKAESEVAP protein) GAPEEVPAPATELEESVSSPEGSVSVTQDGSAAEHQGAPDDPIGLFLMRPQDGEV TVGGSIVESARVAGASLLKPPVVKWFKGKWVDLSSKVGQHLQLHDSYDRASKVYL FELHITDAQTTSAGGYRCEVSTKDKFDSCNFNLTVHEAIGSGDLDLRSAFRRTSLA GAGRRTSDSHEDAGTPDFSSLLKKRDSFRRDSKLEAPAEEDVWEILRQAPPSEYE RIAFQHGVEACHRPLKRLKGMKQDEKKSTAFQKKLEPAYQVNKGHKIRLTVELAD PDAEVKWLKNGQEIQMSGSKYIFESVGAKRTLTISQCSLADDAAYQCVVGGEKCS TELFVKEPPVLITRSLEDQLVMVGQRVEFECEVSEEGAQVKWLKDGVELTREETF KYRFKKDGRKHHLIINEATLEDAGHYAVRTSGGQSLAELIVQEKKLEVYQSIADLAV GAKDQAVEKCEVSDENVRGVWLKNGKELVPDNRIKVSHIGRVHKLTIDDVTPADE ADYSFVPEGFACNLSAKLHFMEVKIDFVPRQEPPKIHLDCPGSTPDTIVVVTGNKLR LDVPISGDPAPTVVWQKTVTQGKKASAGPHPDAPEDAGADEEWVEDKKLLCETE GRVRVETTKDRSVFTVEGAEKEDEGVYTVTVKNPVGEDQVNLTVKVIDVPDAPAA PKISNVGEDSCTVQWEPPAYDGGQPVLGYILERKKKKSYRWMRLNEDLLRELSHE ARRMIEGVAYEMRVYAVNAVGMSRPSPASQPFMPIGPPGEPTHLAVEDVSDTTVS LKWRPPERVGAGGLDGYSVEYCQEGCSEWTPALQGLTERRSMLVKDLPTGARLL FRVRAHNVAGPGGPIVTKEPVTVQEILQRPRLQLPRHLRQTIQKKVGEPVNLLIPFQ GKPRPQVTWTKEGQPLAGEEVSIRNSPTDTILFIRAARRTHSGTYQVIVRIENMED KATLILQIVDKPSPPQDIRIVETWGFNVALEWKPPQDDGNTEIWGYTVQKADKKTM EWFTVLEHYRRTHCVVSELIIGNGYYFRVFSHNMVGSSDKAAATKEPVFIPRPGITY EPPKYKALDFSEAPSFTQPLANRSIIAGYNAILCCAVRGSPKPKISWEKNGLDLGED ARFRMECKQGVLTLEIRKPCPYDGGVYVCRATNLQGEAQCECRLEVRVPQ SEQ ID NO: 11 ATGCCGGAGCCGGGTAAAAAGCCGGTTAGCGCGTTCAACAAAAAACCGCGTA (cMyBP-C C0-C7 GCGCGGAAGTGACCGCGGGTAGCGCGGCGGTGTTTGAGGCGGAAACCGAGC domains) GTAGCGGCGTTAAAGTGCGTTGGCAACGTGACGGTAGCGATATTACCGCGAA CGATAAATACGGTCTGGCGGCGGAAGGCAAGCGTCACACCCTGACCGTTCGT GATGCGAGCCCGGACGATCAGGGTAGCTATGCGGTGATCGCGGGCAGCAGC AAGGTGAAATTTGACCTGAAAGTTACCGAGCCGGCTCCGCCGGAAAAAGCGG AAAGCGAGGTGGCGCCGGGTGCGCCGAAGGAAGTTCCGGCGCCGGCGACCG AGCTGGAGGAAAGCGTGAGCAGCCCGGAAGGCAGCGTTAGCGTGACCCAGG ATGGTAGCGCGGCGGAACATCAAGGTGCGCCGGATGATCCGATCGGTCTGTT CCTGATGCGTCCGCAAGACGGTGAGGTTACCGTGGGTGGCAGCATTGTGTTTA GCGCGCGTGTTGCGGGTGCGAGCCTGCTGAAACCGCCGGTGGTTAAGTGGTT CAAGGGCAAATGGGTGGATCTGAGCAGCAAAGTTGGTCAGCACCTGCAACTG CACGACAGCTACGATCGTGCGAGCAAGGTTTACCTGTTCGAACTGCACATTAC CGATGCGCAGACCACCAGCGCGGGTGGCTACCGTTGCGAGGTTAGCACCAAG GACAAATTCGATAGCTGCAACTTTAACCTGACCGTGCACGAAGCGATCGGTAG CGGCGACCTGGATCTGCGTAGCGCGTTTCGTCGTACCAGCCTGGCGGGTGCG GGTCGTCGTACCAGCGACAGCCATGAGGATGCGGGCACCCTGGATTTCAGCA GCCTGCTGAAGAAACGTGATAGCTTTCGTCGTGACAGCAAACTGGAAGCGCCG GCGGAGGAAGACGTTTGGGAGATCCTGCGTCAAGCTCCGCCGAGCGAATACG AGCGTATTGCGTTCCAGCACGGTGTGACCGATCTGCGTGGCATGCTGAAGCG TCTGAAGGGTATGAAGCAGGACGAAAAGAAAAGCACCGCGTTTCAGAAGAAAC TGGAGCCGGCGTATCAAGTGAACAAAGGCCACAAGATCCGTCTGACCGTGGA ACTGGCGGACCCGGATGCGGAAGTGAAATGGCTGAAGAACGGCCAGGAAATC CAAATGAGCGGTAGCAAATACATTTTCGAGAGCGTTGGTGCGAAGCGTACCCT GACCATTAGCCAATGCAGCCTGGCGGACGATGCGGCGTATCAGTGCGTGGTT GGTGGCGAGAAATGCAGCACCGAACTGTTCGTGAAGGAGCCGCCGGTTCTGA TCACCCGTAGCCTGGAAGATCAGCTGGTTATGGTGGGTCAACGTGTGGAATTT GAGTGCGAAGTTAGCGAGGAAGGCGCGCAAGTGAAATGGCTGAAGGACGGTG TTGAGCTGACCCGTGAGGAAACCTTCAAATACCGTTTTAAGAAAGATGGTCGTA AGCACCACCTGATCATTAACGAAGCGACCCTGGAGGATGCGGGTCACTATGC GGTTCGTACCAGCGGTGGCCAGAGCCTGGCGGAACTGATCGTGCAAGAAAAG AAACTGGAAGTGTATCAGAGCATTGCGGATCTGGCGGTGGGTGCGAAAGACC AGGCGGTGTTCAAGTGCGAAGTTAGCGATGAGAACGTTCGTGGTGTGTGGCT GAAAAACGGCAAGGAGCTGGTTCCGGACAACCGTATCAAAGTGAGCCACATTG GTCGTGTTCACAAGCTGACCATCGACGATGTTACCCCGGCGGACGAAGCGGA TTATAGCTTCGTGCCGGAGGGCTTTGCGTGCAACCTGAGCGCGAAACTGCACT TCATGGAAGTGAAGATCGACTTTGTTCCGCGTCAGGAGCCGCCGAAAATTCAT CTGGATTGCCCGGGTAGCACCCCGGACACCATTGTGGTTGTGGCGGGTAACA AACTGCGTCTGGATGTGCCGATTAGCGGCGACCCGGCGCCGACCGTTGTGTG GCAGAAGACCGTGACCCAAGGTAAGAAAGCGAGCACCGGTCCGCACCCGGAT GCGCCGGAGGATGCGGGTGCGGACGAGGAATGGGTTTTCGATAAGAAACTGC TGTGCGAAACCGAAGGCCGTGTTCGTGTGGAAACCACCAAGGATCGTAGCGTT TTTACCGTGGAGGGCGCGGAGAAAGAAGACGAGGGTGTTTACACCGTTACCG TGAAGAACCCGGTGGGTGAAGACCAGGTTAACCTGACCGTTAAAGTTATTGAT GTTCCGGATGCGCCGGCGGCGCCGAAGATTAGCAACGTGGGTGAAGATAGCT GCACCGTTCAATGGGAGCCGCCGGCGTATGATGGTGGCCAGCCGGTGCTGG GCTATATCCTGGAGCGTAAGAAAAAGAAAAGCTATCGTTGGATGCGTCTGAAC TTCGACCTGCTGCGTGAACTGAGCCACGAGGCGCGTCGTATGATTGAAGGTGT TGCGTACGAGATGCGTGTTTATGCGGTGAACGCGGTTGGTATGAGCCGTCCG AGCCCGGCGAGCCAGCCGTTTATGCCGATTGGTCCGCCGGGTGAACCGACCC ACCTGGCGGTGGAGGACGTTAGCGATACCACCGTGAGCCTGAAATGGCGTCC GCCGGAACGTGTTGGTGCGGGTGGCCTGGATGGCTACAGCGTGGAATATTGC CAAGAGGGCTGCAGCGAATGGACCCCGGCGCTGCAGGGTCTGACCGAGCGT ACCAGCATGCTGGTTAAAGACCTGCCGACCGGTGCGCGTCTGCTGTTCCGTGT GCGTGCGCATAACGTTGCGGGTCCGGGTGGCCCGATCGTGACCAAGGAACCG GTTACCGTGCAGGAGATT SEQ ID NO: 12 MPEPGKKPVSAFNKKPRSAEVTAGSAAVFEAETERSGVKVRWQRDGSDITANDK (cMyBP-C C0-C7 YGLAAEGKRHTLTVRDASPODQGSYAVIAGSSKVKFDLKVTEPAPPEKAESEVAP domains) GAPKEVPAPATELEESVSSPEGSVSVTQDGSAAEHQGAPDDPIGLFLMRPQDGEV TVGGSIVFSARVAGASLLKPPVVKWFKGKWVDLSSKVGQHLQLHDSYDRASKVYL FELHITDAQTTSAGGYRCEVSTKDKFDSCNFNLTVHEAIGSGDLDLRSAFRRTSLA GAGRRTSDSHEDAGTLDFSSLLKKRDSFRRDSKLEAPAEEDVWEILRQAPPSEYE RIAFQHGVTDLRGMLKRLKGMKQDEKKSTAFQKKLEPAYQVNKGHKIRLTVELAD PDAEVKWLKNGQEIQMSGSKYIFESVGAKRTLTISQCSLADDAAYQCVVGGEKCS TELFVKEPPVLITRSLEDQLVMVGQRVEFECEVSEEGAQVKWLKDGVELTREETF KYRFKKDGRKHHLIINEATLEDAGHYAVRTSGGQSLAELIVQEKKLEVYQSIADLAV GAKDQAVEKCEVSDENVRGVWLKNGKELVPDNRIKVSHIGRVHKLTIDDVTPADE ADYSFVPEGFACNLSAKLHFMEVKIDFVPRQEPPKIHLDCPGSTPDTIVVVAGNKL RLDVPISGDPAPTVVWQKTVTQGKKASTGPHPDAPEDAGADEEWVEDKKLLCET EGRVRVETTKDRSVETVEGAEKEDEGVYTVTVKNPVGEDQVNLTVKVIDVPDAPA APKISNVGEDSCTVQWEPPAYDGGQPVLGYILERKKKKSYRWMRLNFDLLRELSH EARRMIEGVAYEMRVYAVNAVGMSRPSPASQPFMPIGPPGEPTHLAVEDVSDTTV SLKWRPPERVGAGGLDGYSVEYCQEGCSEWTPALQGLTERTSMLVKDLPTGARL LERVRAHNVAGPGGPIVTKEPVTVQEI SEQ ID NO: 13 MPEPGKKPVSAFNKKPRSAEVTAGSAAVFEAETERSGVKVRWQRDGSDITANDK (C0-C7-SC-ST- YGLAAEGKRHTLTVRDASPDDQGSYAVIAGSSKVKFDLKVTEPAPPEKAESEVAP C8-C10) GAPKEVPAPATELEESVSSPEGSVSVTQDGSAAEHQGAPDDPIGLFLMRPQDGEV TVGGSIVFSARVAGASLLKPPVVKWFKGKWVDLSSKVGQHLQLHDSYDRASKVYL FELHITDAQTTSAGGYRCEVSTKDKFDSCNFNLTVHEAIGSGDLDLRSAFRRTSLA GAGRRTSDSHEDAGTLDFSSLLKKRDSFRRDSKLEAPAEEDVWEILRQAPPSEYE RIAFQHGVTDLRGMLKRLKGMKQDEKKSTAFQKKLEPAYQVNKGHKIRLTVELAD PDAEVKWLKNGQEIQMSGSKYIFESVGAKRTLTISQCSLADDAAYQCVVGGEKCS TELFVKEPPVLITRSLEDQLVMVGQRVEFECEVSEEGAQVKWLKDGVELTREETF KYRFKKDGRKHHLIINEATLEDAGHYAVRTSGGQSLAELIVQEKKLEVYQSIADLAV GAKDQAVFKCEVSDENVRGVWLKNGKELVPDNRIKVSHIGRVHKLTIDDVTPADE ADYSFVPEGFACNLSAKLHFMEVKIDFVPRQEPPKIHLDCPGSTPDTIVVVAGNKL RLDVPISGDPAPTVVWQKTVTQGKKASTGPHPDAPEDAGADEEWVFDKKLLCET EGRVRVETTKDRSVFTVEGAEKEDEGVYTVTVKNPVGEDQVNLTVKVIDVPDAPA APKISNVGEDSCTVQWEPPAYDGGQPVLGYILERKKKKSYRWMRLNFDLLRELSH EARRMIEGVAYEMRVYAVNAVGMSRPSPASQPFMPIGPPGEPTHLAVEDVSDTTV SLKWRPPERVGAGGLDGYSVEYCQEGCSEWTPALQGLTERTSMLVKDLPTGARL LFRVRAHNVAGPGGPIVTKEPVTVQEISGDSATHIKFSKRDEDGKELAGATMELRD SSGKTISTWISDGQVKDFYLYPGKYTFVETAAPDGYEVATAITFTVNEQGQVTVNG KATKGGAHIVMVDAYKPTKLQRPRLQLPRHLRQTIQKKVGEPVNLLIPFQGKPRPQ VTWTKEGQPLAGEEVSIRNSPTDTILFIRAARRTHSGTYQVTVRIENMEDKATLILQI VDKPSPPQDIRIVETWGFNVALEWKPPQDDGNTEIWGYTVQKADKKTMEWFTVLE HYRRTHCVVSELIIGNGYYFRVFSHNMVGSSDKAAATKEPVFIPRPGITYEPPKYKA LDFSEAPSFTQPLANRSIIAGYNAILCCAVRGSPKPKISWFKNGLDLGEDARFRMFC KQGVLTLEIRKPCPYDGGVYVCRATNLQGEAQCECRLEVRVPQ
Applications

(30) The methods and/or compositions of present invention may be used as a research tool, for example to study cMyBP-C (e.g., to study its function, its features, drug interactions, mutations that lead to diseases, effects of introduced mutations, effects of post-translational modification such as phosphorylation, effects of a longer or shorter domains, effects of stiffer or more compliant domains, etc.), to study diseases such as hypertrophic cardiomyopathy, or for other research purposes. The present invention may also be applied to other proteins, such as skeletal muscle MyBP-C isoforms or other sarcomere proteins. There is also the potential to develop cell lines (e.g., stem cells) with a cassette (e.g., TEV/SpyTag cassette) in cMyBP-C or other sarcomere proteins.

(31) In certain embodiments the methods and/or compositions of the present invention are used in motility assays. For example, in certain embodiments, recombinant cMyBP-C proteins of the present invention may be used in motility assays to test the effects of certain drugs. In certain embodiments, recombinant cMyBP-C proteins of the present invention may be used in motility assays for the purpose of evaluating the effects of mutations or changes in the cMyBP-C protein. The present invention is not limited to these uses for the recombinant cMyBP-C proteins or purposes of motility assays. Standard motility assays have coverslipped slides with attached myosin heads (se FIG. 4), wherein the attached myosin heads can propel fluorescently labeled filaments of actin. The recombinant cMyBP-C proteins of the present invention would be more suitable for motility assays that feature isolated thick filaments linked to a coverslip (see FIG. 5), which allow myosin heads to be in a configuration that is more physiologically relevant and allow for the appropriate positioning and function of cMyBP-C.

(32) Thick filaments for use in such assays can be isolated from systems expressing recombinant cMyBP-C proteins that are constructed using methods of the present invention (e.g., the thick filaments can be isolated from Spy-C mice, from permeabilized myocytes, etc.). These isolated thick filaments can be attached to a coverslip as demonstrated in Previs et al. (Science 2012, 337:1215-1218), and the filaments can be used to test the effects of particular drug (e.g., to see if the drug is an activator or an inhibitor, etc.). In certain embodiments, the filaments featuring recombinant cMyBP-C proteins are used for research purposes, e.g., to evaluate the function or effects of mutations or changes to the cMyBP-C protein. In certain embodiments, force measurements may be used to assess the results of a drug screen or to test the effects of mutations or changes to the cMyBP-C protein. In certain embodiments, the speed of the actin molecules may be assessed to determine the results of a drug screen or to test the effects of mutations or changes to the cMyBP-C protein.

(33) Various modifications of the invention, in addition to those described herein, will be apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the appended claims. Each reference cited in the present application is incorporated herein by reference in its entirety.

(34) Although there has been shown and described the preferred embodiment of the present invention, it will be readily apparent to those skilled in the art that modifications may be made thereto which do not exceed the scope of the appended claims. Therefore, the scope of the invention is only to be limited by the following claims. Reference numbers recited in the claims are exemplary and for ease of review by the patent office only, and are not limiting in any way. In some embodiments, the figures presented in this patent application are drawn to scale, including the angles, ratios of dimensions, etc. In some embodiments, the figures are representative only and the claims are not limited by the dimensions of the figures. In some embodiments, descriptions of the inventions described herein using the phrase “comprising” includes embodiments that could be described as “consisting of”, and as such the written description requirement for claiming one or more embodiments of the present invention using the phrase “consisting of” is met.

(35) The reference numbers recited in the below claims are solely for ease of examination of this patent application, and are exemplary, and are not intended in any way to limit the scope of the claims to the particular features having the corresponding reference numbers in the drawings.