Use of tetrazolinones for combating resistant phytopathogenic fungi on fruits

Abstract

The present invention relates to the use of use of a tetrazolinone fungicide for combating phytopathogenic fungi on fruits, such fungi containing a G143A mutation in the mitochondrial cytochrome b gene conferring resistance to Qo inhibitors.

Claims

1. A method for combating phytopathogenic fungi on fruits, wherein the fungi, their habitat, breeding grounds, their locus or the plants to be protected against fungal attack, the soil or plant propagation material are treated with an effective amount of a compound of 1-[2-[[1-(4-chlorophenyl)pyrazol-3-yl]oxymethyl]-3-methyl-phenyl]-4-methyl-tetrazol-5-one (compound I), such fungi containing a G143A mutation in the mitochondrial cytochrome b gene conferring resistance to Qo inhibitors.

2. The method of claim 1, wherein the fruit plant is apple.

3. The method of claim 2, wherein the phytopathogenic fungi is Venturia inaequalis.

4. The method of claim 1, wherein the fruit plant is grape.

5. The method of claim 4, wherein the phytopathogenic fungi is Uncinula necator.

6. The method of claim 4, wherein the phytopathogenic fungi is Plasmopara viticola.

7. The method of claim 1, wherein compound I as defined in claim 1 is applied in form of a mixture with second compound II, which is selected from the group consisting of 2-[4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl]-1-(1,2,4-triazol-1-yl)propan-2-ol, 2-[4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl]-3-methyl-1-(1,2,4-triazol-1-yl)butan-2-ol, 1[4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl]-1-cyclopropyl-2-(1,2,4-triazol-1-yl)ethanol, difenoconazole, penconazole, tetraconazole, myclobutanil, fluxapyroxad, boscalid, fluopyram, folpet, mancozeb, metiram, dithianon, folpet, sulfur, copper, metrafenone, ametoctradin, dimethomorph, oxathiapiproline, cyazofamid, cyprodinil, pyrimethanil, iprodione, fludioxonil and fluopicolide.

8. The method of claim 7, wherein the ratio by weight of compound I and second compound II is 500:1 to 1:500.

9. The method of claim 7, wherein the fruit plant is apple.

10. The method of claim 7, wherein the fruit plant is grape.

11. The method of claim 10, wherein the phytopathogenic fungi is Uncinula necator.

12. The method of claim 10, wherein the phytopathogenic fungi is Plasmopara viticola.

13. The method of claim 9, wherein the phytopathogenic fungi is Venturia inaequalis.

14. The method of claim 7, wherein compound II is selected from the group consisting of 2-[4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl]-1-(1,2,4-triazol-1-yl)propan-2-ol, 2-[4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl]-3-methyl-1-(1,2,4-triazol-1-yl)butan-2-ol, 1-[4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl]-1-cyclopropyl-2-(1,2,4-triazol-1-yl)ethanol, fluxapyroxad, boscalid, metiram, dithianon, metrafenone, ametoctradin, dimethomorph and pyrimethanil.

Description

EXAMPLES

(1) Activity against grape powdery mildew caused by Uncinula necator (UNCINE)

(2) The active compounds were formulated separately. Both compounds were applied on plants at the same concentrations of 100, 25 and 5 ppm. The spray broths were prepared as aqueous solutions based on a 6% EC of compound I according to the present invention and a 5% Aceton/water solution of compound I-213 from prior art (WO2013/092224).

(3) The spray solutions were applied until runoff using an experimental glasshouse spray machine at a water volume equivalent to 1.000 L/ha. Treated plants were placed (together with untreated plants as a reference for infection success) in a glasshouse chamber at approx. 18° C. and 50% rH for two days. Two days after treatment, the plants were split into two groups. They were artificially inoculated using an aqueous conidia suspension of 0.1 to 0.5 E05 conidia/ml. One part was inoculated using a Qol-sensitive isolate, the other part using a Qol-resistant isolate carrying the target mutation G143A. After the artificial inoculation, the plants were placed in a glasshouse chamber at 21° C. and 55% rH for 19 days. The assessment of the infection was done 19 days after the inoculation and reported as % infected leaf area on two leaves which were fully covered by the spray application.

(4) TABLE-US-00003 Conc. Infection (%) Sens. Infection (%) Res. Compound (ppm) Uncinula necator Uncinula necator compound I-213 100 21.3 55.0 (WO13/092224) 25 21.7 66.7 5 40.5 68.0 compound I of the 100 0.0 0.1 present invention 25 0.4 1.2 5 0.3 5.6

(5) The comparison of compound I according to the present invention with compound I-213 from prior art (WO2013/092224) show the unexpected superior activity of compound I (1-[2-[[1-(4-chlorophenyl)pyrazol-3-yl]oxymethyl]-3-methyl-phenyl]-4-methyl-tetrazol-5-one) for resistant phytopathogenic Uncinula necator.