TRICYCLIC COMPOUNDS AND PROCESS FOR PREPARATION THEREOF

Abstract

The present invention discloses tricyclic compounds of formula (I) or salt thereof and their process for synthesis. Further, the present invention relates to the use of these novel tricyclic compounds of formula (I) or salt thereof as insect repellents.

##STR00001##

Claims

1. A compound of formula (I) ##STR00024## wherein, R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sup.6, R.sup.7, R.sup.8 is selected from group consisting of hydrogen, alkyl (C.sub.1 to C.sub.4), COOR, COOH COR, CONRR, CH.sub.2OR, NRR wherein, any two of R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sup.6, R.sup.7, R.sup.8 may form a 3 to 8 membered carbocyclic ring which may optionally be substituted or may contain a heteroatom selected from O or N; X is selected from C═O; C═S or C—R—R; R is selected from hydrogen, alkyl (C.sub.1 to C.sub.4); wherein any two R may form a 3 to 8 membered carbocyclic ring which may optionally be substituted or may contain a heteroatom selected from O or N; represents a single or double bond; either of the ring in formula (I) may additionally contain at least one carbonyl group or salt thereof.

2. The compound as claimed in claim 1, wherein the compounds of formula (I) are selected from the group consisting of: i. (1aR,1bR,2R,6aS)-1,1,1b,2-Tetramethyl-1,1a,1b,2,3,6a-hexahydrocyclopropa[a]indene-4,6-dione (1a), ii. Ethyl(1R,1aS,1bR,2R,6aR)-1b,2-dimethyl-4,6-dioxo-1,1a,1b,2,3,4,6,6a-octahydrocyclopropa[a]indene-1-carboxylate (1b), iii. (1aR,6aR)-1,1-Dimethyl-1a,1b,2,3,6,6a-hexahydrocyclopropa[a]-inden-4(1H)-one (1c), iv. (1aR,6aR)-1,1,5-trimethyl-1a,1b,2,3,6,6a hexahydrocyclopropa[a]inden-4(1H)-one (1d), v. (1aR,1bS,6aR)-1,1,2-Trimethyl-1a,1b,2,3,6,6a-hexahydrocyclo propa[a]inden-4(1H)-one (1e), vi. (1aR,1bS,6aR)-2-Ethyl-1,1-dimethyl-1a,1b,2,3,6,6a-hexahydrocyclo propa[a]inden-4(1H)-one (10, vii. (1aR,1bS,6aR)-1,1-Dimethyl-2-propyl-1a,1b,2,3,6,6a-hexahydrocyclo propa[a]inden-4(1H)-one (1g), viii. (1aR,1bR,6aR)-1,1,2,2-Tetramethyl-1a,1b,2,3,6,6a-hexahydrocyclopropa[a]inden-4(1H)-one (1h).

3. The process for the preparation of compounds of formula (I) as claimed in claim 1, wherein said process comprising the steps of: a) adding boron trifluoride diethyl etherate (BF.sub.3.OEt.sub.2) to a solution of diene compounds of formula 4 and (E)-2-methylbut-2-enal of formula 3 in Dichloromethane (CH.sub.2Cl.sub.2) followed by stirring the reaction for the period ranging from 10 to 12 h at temperature in the range of −78° C. to 30° C. to afford compound of formula 6; or b) adding Grubbs second-generation catalyst to a solution of compound of formula 6 of step (a) followed by stirring the mixture for 22 to 24 h temperature in the range of 25 to 30° C. to afford compound of formula 7; c) adding Manganese triacetate dehydrate [Mn(OAC).sub.3.2H.sub.2O] and tert-butyl hydroperoxide (.sup.t−BuOOH) to a solution of compound of formula 7 of step (b) in ethyl acetate (EtOAc) followed by stirring the reaction mixture for 22 to 24 h at temperature 25 to 30° C. to give compound of formula 8a; d) adding 1,8-Diazabicyclo[5.4.0]undec-7-ene (DBU) to a suspension of (ethoxycarbonylmethyl)-dimethylsulfonium bromide in chloroform (CHCl.sub.3) to obtain a mixture followed by addition of solution of compound of formula 8a of step (c) in CHCl.sub.3 and stirring the reaction mixture to afford compound of formula 1b; e) adding zinc dust to a stirred solution of dienedione of formula 8a of step (c) in acetic acid followed by stirring for 20 to 30 min at temperature in the range of 25 to 30° C. to afford enone compound of formula 9; f) adding ethylene glycol and p-Toluenesulfonic acid (PTSA) to a solution of compound of formula 9 of step (e) in benzene followed by refluxing for 50 to 60 minutes at temperature in the range of 80 to 85° C. to afford compound of formula 10; g) adding the solution of .sup.t−BuLi in pentane and isopropyldiphenylsulfonium tetrafluoroborate in THF to a solution of compound of formula 10 of step (f) followed by cooling the reaction mixture to (−)15 to (−)20° C. for 50 to 60 minutes quenching the reaction mixture to give compound of formula 11; h) adding 2-iodoxybenzoic acid (IBX) and trifluoroacetic acid (TFA) to a solution of ketone of formula 11 of step (f) in dimethyl sulfoxide (DMSO) followed by stirring the reaction mixture at 70 to 80° C. for 20 to 24 h to afford compound of formula (I).

4. The process for the preparation of compounds of formula (I) as claimed in claim 3, wherein the step (c) is carried out under nitrogen atmosphere.

5. The process for the preparation of compounds of formula (I) as claimed in claim 3, wherein the process further comprises adding a solution of tert-Butyllithium (.sup.tBuLi) in pentane and isopropyldiphenylsulfonium tetrafluoroborate in THF to a solution of compound of formula 8a of step (c) followed by cooling the mixture to −30° C. for 1 h to afford Nardoaristolone B.

6. The process for the preparation of compounds of formula (I) as claimed in claim 1, wherein said process comprises adding potassium tert-butoxide (KO.sup.tBu) and ketone compound to a stirred solution of compound of formula 14 ((1R,5S)-6,6-dimethylbicyclo[3.1.0]hexan-3-one) and sodium sulfate (Na.sub.2SO.sub.4) in tertiary butanol followed by stirring the reaction mixture for the period ranging from 0.5 h 6 h at temperature 25° C.-60° C. to afford compounds of formula (I).

7. The process for the preparation of compounds of formula (I) as claimed in claim 7, wherein ketone compounds is selected from methyl vinyl ketone, pent-1-en-3-one, (E)-pent-3-en-2-one, (E)-hex-3-en-2-one, (E)-hept-3-en-2-one, 4-methylpent-3-en-2-one.

8. The compound as claimed in claim 1, wherein the compounds are used as insect repellents.

9. The compound as claimed in claim 1, wherein protection period of the compounds of formula (I) against mosquito bites is in the range of 1.23 h to 6.46 h at the concentration in the range of 0.25 to 0.50 mg/cm.sup.2.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0044] The present invention provides tricyclic compounds of formula (I) and process of synthesis thereof which are useful as insect repellents.

[0045] The present invention provides 3/5/6 tricyclic compounds of formula (I),

##STR00003##

wherein,
R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sup.6, R.sup.7, R.sup.8 is selected from the group consisting of hydrogen, alkyl (C.sub.1-C.sub.4), COOR, COOH, COR, CONRR, CH.sub.2OR, NRR;
wherein any two of R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sup.6, R.sup.7, R.sup.8 may form a 3 to 8 membered carbocyclic ring which may optionally be substituted or may contain a heteroatom selected from O or N;
X is selected from C═O; C═S or CRR;
R is selected from hydrogen, alkyl (C.sub.1-C.sub.4);
wherein any two R may form a 3 to 8 membered carbocyclic ring which may optionally be substituted or may contain a heteroatom selected from O or N;

[0046] represents a single or double bond;

[0047] Either of the ring in formula (I) may additionally contain at least one carbonyl group or salt thereof.

[0048] The tricyclic compounds of formula (I) are selected from the group consisting of: [0049] (1aR,1bR,2R,6aS)-1,1,1b,2-Tetramethyl-1,1a,1b,2,3,6a-hexahydro-cyclopropa[a]indene-4,6-dione (1a), [0050] Ethyl(1R,1aS,1 bR,2R,6aR)-1b,2-dimethyl-4,6-dioxo-1,1a,1b,2,3,4,6,6a-octahydrocyclopropa[a]indene-1-carboxylate (1b), [0051] (1aR,6aR)-1,1-Dimethyl-1a,1b,2,3,6,6a-hexahydrocyclopropa[a]-inden-4(1H)-one (1c), [0052] (1aR,6aR)-1,1,5-trimethyl-1a,1b,2,3,6,6a hexahydrocyclopropa[a]inden-4(1H)-one (1d), [0053] (1aR,1bS,6aR)-1,1,2-Trimethyl-1a,1b,2,3,6,6a-hexahydrocyclo propa[a]inden-4(1H)-one (1e), [0054] (1aR,1bS,6aR)-2-Ethyl-1,1-dimethyl-1a,1b,2,3,6,6a-hexahydrocyclo propa[a]inden-4(1H)-one (1f), [0055] (1aR,1bS,6aR)-1,1-Dimethyl-2-propyl-1a,1b,2,3,6,6a-hexahydrocyclo propa[a]inden-4(1H)-one (1g), [0056] (1aR,1bR,6aR)-1,1,2,2-Tetramethyl-1a,1b,2,3,6,6a-hexahydro cyclo propa[a]inden-4(1H)-one (1h).

[0057] The present invention provides a process for the synthesis of 3/5/6 tricyclic compounds formula (I) from tiglic aldehyde comprising the steps of: [0058] a) adding boron trifluoride diethyl etherate (BF.sub.3.OEt.sub.2) to a solution of diene compounds of formula 4 and (E)-2-methylbut-2-enal of formula 3 in Dichloromethane (CH.sub.2Cl.sub.2) to afford compound of formula 6; or [0059] Adding BF.sub.3.OEt.sub.2 to a solution of diene of formula 5 and (E)-2-methylbut-2-enal of formula 3 in CH.sub.2Cl.sub.2 followed by addition of methyl triphenylphosphonium bromide in dry tetrahydrofuran (THF) and potassium tert-butoxide to the reaction mixture followed by addition of diisobutylaluminium hydride (DIBAL-H) to afford compound of formula 6; [0060] b) adding Grubbs second-generation catalyst to a solution of compound of formula 6 of step (a) to afford compound of formula 7; [0061] c) Adding Manganese triacetate dehydrate [Mn(OAC).sub.3.2H.sub.2O] and tert-butyl hydroperoxide (.sup.t−BuOOH) to a solution of compound of formula 7 of step (b) in ethyl acetate (EtOAc) followed by stirring the reaction mixture to give compound of formula 8a; [0062] d) adding 1,8-Diazabicyclo[5.4.0]undec-7-ene (DBU) to a suspension of (ethoxycarbonylmethyl)-dimethylsulfonium bromide in chloroform (CHCl.sub.3) to obtain a mixture followed by addition of solution of compound of formula 8a of step (c) in CHCl.sub.3 and stirring the reaction mixture to give compound of formula 1b; [0063] e) adding zinc dust to a stirred solution of dienedione of formula 8a of step (c) in acetic acid to give compound of formula 9; [0064] f) adding ethylene glycol and p-Toluenesulfonic acid (PTSA) to a solution of compound of formula 9 of step (d) followed by refluxing and quenching to give compound of formula 10; [0065] g) adding the solution of .sup.t−BuLi in pentane and isopropyldiphenylsulfonium tetrafluoroborate in THF to a solution of compound of formula 10 of step (e) and quenching the reaction mixture to give compound of formula 11; [0066] h) adding 2-iodoxybenzoic acid (IBX) and TFA to a solution of ketone of formula 11 of step (f) in dimethyl sulfoxide (DMSO) and stirring the reaction mixture followed by quenching to give compound of formula (I).

[0067] The above process for the synthesis of 3/5/6 tricyclic compounds of formula (I) is shown in scheme 1.

[0068] The present invention provides the process for synthesis of 3/5/6 tricyclic compounds of formula (I) from compound of formula 14

##STR00004##

comprising the steps of: [0069] a) adding potassium tert-butoxide (KO.sup.tBu) and ketone compound to a stirred solution of compound of formula 14 and sodium sulfate (Na.sub.2SO.sub.4) in dry tertiary butanol to obtain the reaction mixture; [0070] b) stirring the reaction mixture for the period ranging from 0.5 h 6 h at temperature 25° C.-60° C. followed by quenching to give compound of formula (I). [0071] c) The ketone compound is selected from methyl vinyl ketone, pent-1-en-3-one, (E)-pent-3-en-2-one, (E)-hex-3-en-2-one, (E)-hept-3-en-2-one, 4-methylpent-3-en-2-one.

[0072] The above process for synthesis of 3/5/6 tricyclic compounds is as shown in Scheme 2.

[0073] The present invention provides a process for the synthesis of Nardoaristolone B comprising the steps of: [0074] a) Adding boron trifluoride diethyl etherate (BF.sub.3.OEt.sub.2) to a solution of diene compounds of formula 4 and (E)-2-methylbut-2-enal of formula 3 in dry CH.sub.2Cl.sub.2 to give compound of formula 6; or [0075] b) Adding BF.sub.3.OEt.sub.2 to a solution of diene of formula 5 and (E)-2-methylbut-2-enal of formula 3 in dry Dichloromethane (CH.sub.2Cl.sub.2) followed by addition of suspension of methyl triphenylphosphonium bromide in dry THF and potassium tert-butoxide to the reaction mixture followed by addition of Diisobutylaluminium hydride (DIBAL) to give compound of formula 6; [0076] c) Adding Grubbs' second-generation catalyst to a solution of compound of formula 6 of step (a) to give compound of formula 7; [0077] d) Adding molecular sieves, Manganese triacetate dehydrate [Mn(OAC).sub.3.2H.sub.2O] and tert-butyl hydroperoxide (.sup.t−BuOOH) to a solution of compound of formula 7 of step (b) in ethyl acetate (EtOAc) followed by stirring the reaction mixture to give compound of formula 8a; [0078] e) Adding a solution of tert-Butyllithium (′BuLi) in pentane and isopropyldiphenylsulfonium tetrafluoroborate in THF to a solution of compound of formula 8a and quenching the resulting mixture to give desired product Nardoaristolone B.

[0079] The above process is shown in Scheme 3.

[0080] The tricyclic compounds of present invention are used as insect repellents.

EXAMPLES

[0081] Following examples are given by way of illustration and therefore should not be construed to limit the scope of the invention.

Example 1

1a) Synthesis of (3R,4S,5R)-3-allyl-4,5-dimethyl-4-vinylcyclohex-1-ene (6)

[0082] ##STR00005##

[0083] To a solution of diene 4 (100 mg, 0.892 mmol) and (E)-2-methylbut-2-enal (0.18 mL, 1.78 mmol) in dry CH.sub.2Cl.sub.2 (20 mL) was added BF.sub.3.OEt.sub.2 (0.16 mL, 0.16 mmol) dropwise at −78° C. The mixture was allowed to warm to 25° C. and was stirred for 10 h at same temperature. The CH.sub.2Cl.sub.2 layer was washed with saturated NaHCO.sub.3 (3×10 mL) followed by H.sub.2O (5 mL) and brine (5 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated in vacuo. The crude material obtained after the removal of solvent was immediately used for next step. To a suspension of methyl triphenylphosphonium bromide (640 mg, 1.78 mmol) in dry THF (10 mL) was added potassium tert-butoxide (200 mg, 1.78 mmol) at 0° C. After 30 minutes, the solution became canary yellow color, to that above crude bis-aldehyde in THF (10 mL) was added and allowed to stir at 0° C. for 1 h. The reaction was quenched with NH.sub.4Cl (5.0 ml) and extracted with petroleum ether (2×30 mL). Combined organic layer was washed with water (15 mL), brine (15 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated in vacuo. Purification by column chromatography (silica gel 100-200, petroleum ether) afforded 6 (15 mg, 10%) as colorless oil. .sup.1H NMR (400 MHz, CDCl.sub.3) δ 6.01-5.94 (m, 1H), 5.86-5.77 (m, 1H), 5.62-5.61 (m, 2H), 5.05-4.99 (m, 4H), 2.31-2.19 (m, 2H), 1.91-1.84 (m, 2H), 1.72-1.64 (m, 2H), 1.03 (s, 3H), 0.86 (d, J=6.8 Hz, 3H); .sup.13C NMR (100 MHz, CDCl.sub.3) δ 146.1, 138.3, 129.1, 125.1, 115.7, 112.3, 43.9, 40.9, 35.8, 35.1, 31.6, 19.3, 16.4; HRMS (ESI) calcd for C.sub.13H.sub.20 [M−H] 175.1481, found 175.1480.

1b) Synthesis of Ethyl 2-((1R,5R,6S)-6-formyl-5,6-dimethylcyclohex-2-en-1-yl) acetate (S-I)

[0084] ##STR00006##

[0085] To a solution of diene 5 (6.0 g, 0.042 mol) and (E)-2-methylbut-2-enal (10.3 mL, 0.107 mmol) in dry CH.sub.2Cl.sub.2 (200 mL) was added BF.sub.3.OEt.sub.2 (10.6 mL, 0.085 mol) dropwise at −78° C. The mixture was allowed to warm to 25° C. and was stirred for 12 h at same temperature. The CH.sub.2Cl.sub.2 layer was washed with saturated NaHCO.sub.3 (3×50 mL) followed by H.sub.2O (50 mL) and brine (50 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated in vacuo. The crude material obtained after the removal of solvent was purified by column chromatography (silica gel 100-200, 1.0:9.0 ethyl acetate:petroleum ether) to afford S-I (7.3 g, 76%) as light yellow oil. IR (neat, cm.sup.−1) 2978, 1732, 1174; .sup.1H NMR (400 MHz, CDCl.sub.3) δ=9.60 (s, 1H), 5.71-5.66 (m, 1H), 5.64-5.97 (m 1H), 4.13 (q, J=7.2 Hz, 2H), 2.65-2.59 (m, 1H), 2.45 (dd, J=5.4 Hz, 15.8 Hz, 1H), 2.37-2.30 (m, 1H), 2.25-2.18 (m, 1H), 2.17-2.05 (m, 1H), 1.79-1.72 (m, 1H), 1.25 (t, J=7.2 Hz, 3H), 1.08 (s, 3H), 0.93 (d, J=6.8 Hz, 3H); .sup.13C NMR (100 MHz, CDCl.sub.3) δ=206.6, 172.6, 127.4, 126.1, 60.4, 49.6, 37.6, 35.6, 30.65, 29.5, 15.7, 15.6, 13.9;

1c) Synthesis of Ethyl 2-((1R,5R,6S)-5,6-dimethyl-6-vinylcyclohex-2-en-1-yl) acetate (S-II)

[0086] ##STR00007##

[0087] To a suspension of methyl triphenylphosphonium bromide (26.8 g, 0.075 mol) in dry THF (60 mL) was added potassium tert-butoxide (7.6 g, 0.068 mol) at 0° C. After 30 minutes, the solution became canary yellow color, to that aldehyde S-I (5.1 g, 0.022 mol) in THF (30 mL) was added and allowed to stirred at 0° C. for 1 h. The reaction was quenched with brine (30 ml) and extracted with ethyl acetate (2×50 mL). Combined organic layer was washed with water (30 mL), brine (30 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated in vacuo. Purification by column chromatography (silica gel 100-200, 0.5:9.5 ethyl acetate:petroleum ether) afforded S-II (4.2 g, 84%) as light brown oil. .sup.1H NMR (400 MHz, CDCl.sub.3) δ=5.86 (dd, J. 11.0 Hz, 18.3 Hz, 1H), 5.65-5.53 (m, 2H), 5.06-5.05 (m, 1H), 5.03-5.02 (m, 1H), 4.15-4.09 (q, 2H), 2.48-2.39 (m, 2H), 2.23-2.18 (m, 1H), 2.12-2.07 (m, 1H), 1.82-1.73 (m, 2H), 1.25 (t, J. 7.3 Hz, 3H), 1.02 (s, 3H), 0.87 (d, J=6.4 Hz, 3H); .sup.13C NMR (100 MHz, CDCl.sub.3) δ=173.7, 145.2, 128.6, 126.2, 113.3, 60.4, 41.2, 40.5, 36.7, 34.4, 31.5, 18.8, 16.4, 14.4. HRMS (ESI) calcd for C.sub.14H.sub.22O.sub.2Na.sup.+245.1512, found 245.1508.

1d) Synthesis of 2-((1R,5R,6S)-5,6-Dimethyl-6-vinylcyclohex-2-en-1-yl) acetaldehyde (S-III)

[0088] ##STR00008##

[0089] To a solution of ester S-II (0.5 g, 2.27 mmol) in dry distilled toluene (15 mL) was added DIBAL (1.0 M in toluene, 1.36 mL, 1.36 mmol) at −78° C. dropwise. Stirred at this temperature for 10 min and then added DIBAL (1.0 M in toluene, 1.13 mL, 1.13 mmol) at −78° C. dropwise. After stirring at the same temperature for 0.5 h, the reaction was quenched with methanol (3 mL), diluted with Et.sub.2O (20 mL) and sat. Na/K tartrate (15 mL). The solution was stirred at 25° C. for 2 h. Extracted the solution with ethyl acetate (3×20 mL), and the combined extracts were dried over Na.sub.2SO.sub.4. The crude mixture was passed through small bed of silica gel and eluted with 20% ethyl acetate:petroleum ether. The eluent was concentrated in vacuo to give the aldehyde S-III (0.33 g, 81%) as a colorless oil which is immediately used for next step.

1e) Synthesis of (3R,4S,5R)-3-allyl-4,5-dimethyl-4-vinylcyclohex-1-ene (6)

[0090] ##STR00009##

[0091] To a suspension of methyl triphenylphosphonium bromide (14.5 g, 0.040 mol) in dry THF (40 mL) was added potassium tert-butoxide (4.1 g, 0.037 mol) at 0° C. After 30 minutes, the solution became canary yellow color, to that aldehyde S-III (2.2 g, 0.012 mol) in THF (20 mL) was added and allowed to stirred at 0° C. for 1 h. The reaction was quenched with NH.sub.4Cl (20 ml) and extracted with petroleum ether (2×60 mL). Combined organic layer was washed with water (20 mL), brine (20 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated in vacuo. Purification by column chromatography (silica gel 100-200, petroleum ether) afforded 6 (1.7 g, 79%) as colorless oil. Spectral data was identical with that of above compound 6.

1f) Synthesis of (3aR,4R, 7aR)-3a, 4-Dimethyl-3a,4,5,7a-tetrahydro-1H-indene (7)

[0092] ##STR00010##

[0093] To a solution of 6 (2.0 g, 0.011 mol) in dry CH.sub.2Cl.sub.2 (100 mL) was added Grubbs' second-generation catalyst (480 mg, 5 mol %) at 25° C. After stirring for 24 h, reaction mixture was filtered through celite and filtrate was concentrated in vacuo. The crude material obtained after the removal of solvent was purified by column chromatography (silica gel 100-200, petroleum ether) to afford 7 (1.2 g, 72%) as colorless oil. .sup.1H NMR (400 MHz, CDCl.sub.3) δ 5.79-5.72 (m, 2H), 5.69-5.66 (m, 2H), 2.56-2.49 (m, 1H), 2.25-2.20 (m, 1H), 2.11-2.04 (m, 1H), 1.93-1.87 (m, 1H), 1.78-1.69 (m, 1H), 1.63-1.56 (m, 1H), 0.92 (s, 3H), 0.90 (d, J=6.6 Hz, 3H); .sup.13C NMR (100 MHz, CDCl.sub.3) δ 141.7, 129.3, 128.5, 125.9, 48.0, 47.7, 38.9, 34.1, 31.05, 18.7, 15.8;

1g) Synthesis of (3aR,4R)-3a, 4-dimethyl-1,6-dioxo-3a, 4,5,6-tetrahydro-1H-inden-2-ylium (8a)

[0094] ##STR00011##

[0095] To a solution of diene 7 (1 g, 6.75 mmol) in EtOAc (100 mL) were added 4 Å molecular sieves (2 g) and .sup.t−BuOOH (5.0 M in decane, 6.7 mL, 33.7 mmol) at room temperature. The reaction mixture was stirred for 30 min before Mn(OAc).sub.3.2H.sub.2O (905 mg, 3.37 mmol) was added at 25° C. The resulting mixture was stirred at room temperature (25-30° C.) an under nitrogen atmosphere for 24 h before it was filtered through celite, eluted with EtOAc (30 mL) and concentrated in vacuo. The crude material obtained after the removal of solvent was purified by column chromatography (silica gel 100-200, 2:8 ethyl acetate:petroleum ether) to afford 8a and mono oxidised mixture which was again oxidised using similar reaction condition to give the 9 (725 mg, 61%) as yellow solid. Mp: 70-72° C.; .sup.1H NMR (400 MHz, CDCl.sub.3) δ 7.70 (d, J=5.9 Hz, 1H), 6.35 (d, J=5.9 Hz, 1H), 6.27 (s, 1H), 2.50-2.46 (m, 2H), 2.27-2.21 (m, 1H), 1.22 (s, 3H), 1.13 (d, J=6.4 Hz, 3H); .sup.13C NMR (100 MHz, CDCl.sub.3) δ 199.7, 195.8, 165.0, 160.4, 133.6, 121.8, 46.5, 42.8, 36.3, 18.4, 15.0; HRMS (ESI) calcd for C.sub.11H.sub.13O.sub.2 [M+H].sup.+ 177.0910, found 177.0909.

1h) Synthesis of (1aS,1bR,2R,6aR)-1,1,1b,2-Tetramethyl-1,1a,1b,2,3,6a-hexahydrocyclopropa[a]indene-4,6-dione (Nardoaristolone B)

[0096] ##STR00012##

[0097] A 1.6 M solution of .sup.t−BuLi in pentane (0.55 mL, 0.852 mmol) was added dropwise to a suspension of isopropyldiphenylsulfonium tetrafluoroborate (296 mg, 0.937 mmol) in THF (4 mL) at −78° C. After 30 min, 8a (50 mg, 0.284 mmol) was added as a solution in THF (2 mL). The resulting mixture was maintained at −30° C. for 1 h, quenched with saturated aqueous NH.sub.4Cl (2 mL), and allowed to warm to 25° C. The reaction mixture was then extracted with ethyl acetate (3×5 mL). Combined organic layer was washed with water (5 mL), brine (5 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated in vacuo. Purification by column chromatography (silica gel 100-200, 1.5:8.5 ethyl acetate:petroleum ether) afforded 1a Nardoaristolone B (20 mg, 32%) as yellow solid.

[0098] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 6.18 (s, 1H), 2.40-2.23 (m, 3H), 1.94 (d, J=5.6 Hz, 1H), 1.77 (d, J=5.6 Hz, 1H), 1.17 (s, 3H), 1.13 (s, 3H), 1.09 (s, 3H), 1.06 (d, J=6.6 Hz, 3H); .sup.13C NMR (100 MHz, CDCl.sub.3) δ 201.5, 200.0, 165.1, 123.6, 44.3, 42.3, 42.2, 40.3, 35.5, 32.1, 28.8, 20.8, 17.8, 15.8; HRMS (ESI) calcd for C.sub.14H.sub.19O.sub.2 [M+H].sup.+ 219.1380, found 219.1377.

Example 2

2a) Synthesis of (3aR,4R)-3a,4-Dimethyl-4,5,7,7a-tetrahydro-1H-indene-1,6(3aH)-dione (9)

[0099] ##STR00013##

[0100] To a stirred solution of dienedione 8a (200 mg, 1.13 mmol) in CH.sub.3COOH (10 mL) at 25° C. was added zinc dust (436 mg, 6.81 mmol). The resulting mixture was stirred for 30 min before being filtered on a short pad of Celite and washed with EtOAc (30 mL). The resulting organic layer was concentrated in vacuo and the crude obtained was purified on column chromatography (silica gel 100-200, 2.5:7.5 ethyl acetate:petroleum ether) to afford enone 9 (143 mg, 71%) as white solid. Mp: 68-70° C.

[0101] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 7.75 (d, J=5.8 Hz, 1H), 6.17 (d, J=5.8 Hz, 1H), 2.67-2.62 (m, 1H), 2.53-2.48 (m, 1H), 2.32-2.26 (m, 2H), 2.20-2.14 (m, 1H), 2.07-2.04 (m, 1H), 1.16 (s, 3H), 1.07 (d, J=6.7 Hz, 3H); .sup.13C NMR (100 MHz, CDCl.sub.3) δ 210.0, 209.5, 170.5, 131.2, 51.5, 47.0, 43.1, 38.7, 35.1, 19.8, 15.5; HRMS (ESI) calcd for C.sub.11H.sub.14O.sub.2Na.sup.+201.0886, found 201.0885.

2b) Synthesis of (7R,7aR)-7,7a-Dimethyl-3a,6,7,7a-tetrahydrospiro[indene-5,2′-[1,3]dioxolan]-3(4H)-one (10)

[0102] ##STR00014##

[0103] To a solution of the 9 (120 mg, 0.674 mmol) in benzene (10 mL) was added ethylene glycol (86 mg, 1.34 mmol) and pTSA (12 mg, 0.067 mmol) at room temperature. The reaction was reflux (80-85° C.) for 1 h then quenched by saturated NaHCO.sub.3 (3 ml) and extracted with ethyl acetate (3×5 mL). Combined organic layer was washed with water (5 mL), brine (5 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated in vacuo. Purification by column chromatography (silica gel 100-200, 2.0:8.0 ethyl acetate:petroleum ether) afforded 10 (130 mg, 87%) as white solid; Mp: 62-64° C. .sup.1H NMR (400 MHz, CDCl.sub.3) δ 7.65 (d, J=5.8 Hz, 1H), 6.07 (d, J=5.8 Hz, 1H), 3.98-3.87 (m, 4H), 2.21-2.12 (m, 2H), 1.81-1.77 (m, 1H), 1.74-1.70 (m, 1H), 1.66-1.56 (m, 2H), 1.15 (s, 3H), 0.96 (d, J=6.7 Hz, 3H); .sup.13C NMR (100 MHz, CDCl.sub.3) δ 209.4, 171.1, 130.5, 108.8, 64.4, 64.1, 54.6, 46.6, 39.5, 37.3, 30.1, 18.0, 16.4; HRMS (ESI) calcd for C.sub.13H.sub.18O.sub.3Na.sup.+245.1148, found 245.1143.

2c) Synthesis of (1aR,1bR,2R,6aS)-1,1,1b,2-Tetramethyloctahydro-6H-spiro[cyclopropa[a]indene-4,2′-[1,3]dioxolan]-6-one (11)

[0104] ##STR00015##

[0105] A 1.6 M solution of .sup.t−BuLi in pentane (0.84 mL, 1.34 mmol) was added dropwise to a suspension of isopropyldiphenylsulfonium tetrafluoroborate (465 mg, 1.47 mmol) in THF (6 mL) at −78° C. After 30 min, 10 (100 mg, 0.446 mmol) was added as a solution in THF (4 mL). The resulting mixture was maintained at −20° C. for 1 h, quenched with saturated aqueous NH.sub.4Cl (2 mL), and allowed to warm to 25° C. The reaction mixture was then extracted with ethyl acetate (3×10 mL). Combined organic layer was washed with water (10 mL), brine (10 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated in vacuo. Purification by column chromatography (silica gel 100-200, 1.5:8.5 ethyl acetate:petroleum ether) afforded 11 (59 mg, 50%, 71% brsm) as white solid. .sup.1H NMR (400 MHz, CDCl.sub.3) δ 3.99-3.83 (m, 4H), Hz, 1H), 2.32-2.28 (m, 1H), 2.19-2.17 (m, 1H), 1.94-1.89 (m, 1H), 1.76 (d, J=5.8 Hz, 1H), 1.68 (d, J=5.8 Hz, 1H), 1.50-1.42, (m, 3H), 1.34 (s, 3H), 1.12 (s, 3H), 1.09 (s, 3H), 0.97 (d, J=6.8 Hz, 3H); .sup.13C NMR (100 MHz, CDCl.sub.3) δ 211.8, 107.8, 64.3, 63.9, 53.4, 43.7, 40.5, 40.2, 38.7, 37.2, 29.9, 28.9, 28.2, 18.0, 16.9, 16.4; HRMS (ESI) calcd for C.sub.16H.sub.24O.sub.3Na.sup.+287.1618, found 287.1614.

2d) Synthesis of (1aR,1bR,2R,6aS)-1,1,1b,2-Tetramethyl-1,1a,1b,2,3,6a-hexahydro-cyclopropa[a]indene-4,6-dione (1a)

[0106] ##STR00016##

[0107] To a solution of ketone 11 (40 mg, 0.151 mmol) in DMSO (5 mL) were added IBX (128 mg, 0.454 mmol) and TFA (˜1 drop) at 0° C. After being stirred at 80° C. for 24 h, the reaction mixture was quenched with saturated aqueous NaHCO.sub.3 (2 mL). The aqueous layer was extracted with EtOAc (3×5 mL), the organic layer was washed with saturated aqueous NaHCO.sub.3, water (10 mL), brine (10 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated in vacuo. Purification by column chromatography (silica gel 100-200, 1.5:8.5 ethyl acetate:petroleum ether) afforded 12 (22 mg, 66%) as yellow solid. .sup.1H NMR (400 MHz, CDCl.sub.3) δ 6.12 (s, 1H), 2.46-2.31 (m, 2H), 2.19-2.14 (m, 1H), 2.01 (d, J=5.6 Hz, 1H), 1.94 (d, J=5.6 Hz, 1H), 1.33 (s, 3H), 1.23 (s, 3H), 1.22 (s, 3H), 1.15 (d, J=6.6 Hz, 3H); .sup.13C NMR (100 MHz, CDCl.sub.3) δ 201.6, 200.1, 165.0, 122.1, 42.5, 41.8, 41.5, 41.4, 40.0, 31.5, 29.1, 20.6, 16.4, 15.0; HRMS (ESI) calcd for C.sub.14H.sub.19O.sub.2 [M+H].sup.+ 219.1380, found 219.1378.

Example 3

Synthesis of Ethyl(1R,1aS,1bR,2R,6aR)-1b,2-dimethyl-4,6-dioxo-1,1a,1b,2,3,4,6,6a-octahydrocyclopropa[a]indene-1-carboxylate (1b)

[0108] ##STR00017##

[0109] DBU (0.06 mL, 0.426 mmol) was added to a suspension of (ethoxycarbonylmethyl)-dimethylsulfonium bromide (97 mg, 0.426 mmol) in CHCl.sub.3 (5.0 mL) at room temperature. After 30 min, compound 8a (50 mg, 0.284 mmol) in CHCl.sub.3 (2.0 mL) was added and the solution was allowed to stir for 16 h at 25° C. The organic solvents were evaporated and then partitioned between CH.sub.2Cl.sub.2 (10 mL) and water (5 mL). The organic layer was washed with brine, dried over MgSO4, filtered, and concentrated. The residue was purified using silica gel chromatography (silica gel 100-200, 2.5:7.5 ethyl acetate:petroleum ether) afforded 1b (43 mg, 58%) as colorless oil. .sup.1H NMR (400 MHz, CDCl.sub.3) δ 6.22 (s, 1H), 4.09-4.02 (m, 2H), 2.52-2.48 (m, 1H), 2.42-2.31 (m, 4H), 2.28-2.24 (m, 1H), 1.26 (s, 3H), 1.19 (t, J=7.0 Hz, 3H), 1.15 (d, J=6.1 Hz, 3H); .sup.13C NMR (100 MHz, CDCl.sub.3) δ 199.9, 199.6, 168.8, 161.6, 124.2, 61.9, 43.5, 42.2, 35.4, 35.1, 31.5, 31.0, 21.3, 15.5, 14.0; HRMS (ESI) calcd for C.sub.15H.sub.18O.sub.4Na.sup.+285.1097, found 285.1094.

Example 4

Synthesis of (1aR,6aR)-1,1-Dimethyl-1a,1b,2,3,6,6a-hexahydrocyclopropa[a]-inden-4(1H)-one (1c)

[0110] ##STR00018##

[0111] To a stirred solution of compound 14 (300 mg, 2.42 mmol) and Na.sub.2SO.sub.4 (1.5 g) in dry tert butanol (15 mL) at 25° C. was added catalytic amount of KO′Bu (27 mg, 0.24 mmol) and then methyl vinyl ketone (0.25 mL, 2.90 mmol) slowly in drop wise manner. Stirring was continued for 30 min. Reaction was quenched by the addition of saturated aq.NH.sub.4Cl (5 mL). Organic solvent was evaporated, water (10 mL) and ethyl acetate (15 mL) were added, organic solvent was separated and aqueous layer was extracted with ethyl acetate (3×10 mL), combined organic layer was washed with brine and dried over anhydrous Na.sub.2SO.sub.4. Purification by column chromatography (silica gel 100-200, 1.0:9.0 ethyl acetate:petroleum ether) afforded 1c (275 mg, 65%) as colourless oil. .sup.1H NMR (400 MHz, CDCl.sub.3) δ 5.69 (s, 1H), 2.70-2.63 (m, 1H), 2.45-2.34 (m, 3H), 2.30-2.25 (m, 1H), 2.21-2.17 (m, 1H), 2.03-1.98 (m, 1H), 1.82-1.73 (m, 1H), 1.33-1.29 (m, 1H), 1.04 (s, 3H), 0.99 (s, 3H); .sup.13C NMR (100 MHz, CDCl.sub.3) δ 199.5, 181.1, 120.8, 40.8, 37.8, 34.4, 32.3, 30.4, 29.0, 27.1, 22.8, 14.2. MS: 177.

Example 5

Synthesis of (1aR,6aR)-1,1,5-trimethyl-1a,1b,2,3,6,6a hexahydrocyclopropa[a]inden-4(1H)-one (1d)

[0112] ##STR00019##

[0113] The compound 1d prepared by using similar procedure given in example 3.

[0114] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 2.77-2.71 (m, 1H), 2.51-2.38 (m, 2H), 2.34-2.24 (m, 2H), 2.13-2.10 (m, 1H), 1.78-1.69 (m, 1H), 1.65 (m, 3H), 1.31-1.28 (m, 2H), 1.03 (s, 3H), 0.97 (s, 3H); .sup.13C NMR (100 MHz, CDCl.sub.3) δ 198.9, 174.2, 126.7, 41.4, 38.4, 34.9, 31.4, 30.3, 29.3, 27.2, 22.1, 14.3, 11.6.

Example: 6

Synthesis of (1aR,1bS,6aR)-1,1,2-Trimethyl-1a,1b,2,3,6,6a-hexahydrocyclo propa[a]inden-4(1H)-one (1e)

[0115] ##STR00020##

[0116] The compound 1d prepared by using similar procedure given in example 3.

[0117] IR (film) vmax 1667, 1373, 1236 cm.sup.−1; .sup.1H NMR (400 MHz, CDCl.sub.3) δ 5.71 (s, 1H), 2.70-2.64 (m, 1H), 2.42-2.34 (m, 2H), 2.03-1.96 (m, 2H), 1.29-1.25 (m, 1H), 1.14-1.12 (m, 1H), 1.11 (d, J=5.9 Hz, 3H), 1.08-1.06 (m, 1H), 1.06 (s, 3H), 0.98 (s, 3H); .sup.13C NMR (100 MHz, CDCl.sub.3) δ 200.0, 180.3, 121.2, 48.1, 46.3, 37.0, 32.8, 32.3, 28.6, 27.2, 22.8, 20.7, 14.4; HRMS (ESI) calcd for C.sub.13H.sub.19O [M+H].sup.+ 191.1430, found 191.1429.

Example 7

Synthesis of (1aR,1bS,6aR)-2-Ethyl-1,1-dimethyl-1a,1b,2,3,6,6a hexahydrocyclo propa[a]inden-4(1H)-one (10

[0118] ##STR00021##

[0119] The compound 1d prepared by using similar procedure given in example 3.

[0120] IR (film) vmax 1668, 1368, 1248 cm.sup.−1; .sup.1H NMR (400 MHz, CDCl.sub.3) δ 5.71 (s, 1H), 2.70-2.64 (m, 1H), 2.53-2.49 (m, 1H), 2.38-2.34 (m, 1H), 2.11-2.08 (m, 1H), 1.97-1.90 (m, 1H), 1.87-1.82 (m, 1H), 1.77-1.71 (m, 1H), 1.36-1.25 (m, 2H), 1.15-1.13 (m, 1H), 1.06 (s, 3H), 0.98 (s, 3H), 0.92 (t, J=7.3 Hz, 3H); .sup.13C NMR (100 MHz, CDCl.sub.3) δ 200.2, 180.6, 121.0, 46.1, 43.1, 42.8, 32.7, 32.3, 28.7, 27.4, 27.3, 22.9, 14.4, 10.6; HRMS (ESI) calcd for C.sub.14H.sub.21O [M+H].sup.+ 205.1587, found 205.1584.

Example 8

Synthesis of (1aR,1bS,6aR)-1,1-Dimethyl-2-propyl-1a,1b,2,3,6,6a-hexahydrocyclo propa[a]inden-4(1H)-one (1g)

[0121] ##STR00022##

[0122] IR(film) vmax 1669, 1351, 1233 cm.sup.−1; .sup.1H NMR (400 MHz, CDCl.sub.3) δ 5.67 (s, 1H), 2.65-2.60 (m, 1H), 2.49-2.47 (m, 1H), 2.35-2.30 (m, 1H), 2.17-2.10 (m, 1H), 2.06-2.04 (m, 1H), 1.91-1.86 (m, 2H), 1.63-1.59 (m, 1H), 1.43-1.35 (m, 1H), 1.25-1.19 (m, 2H), 1.12-1.10 (m, 1H), 1.03 (s, 3H), 0.95 (s, 3H), 0.89 (t, J=7.3 Hz, 3H); .sup.13C NMR (100 MHz, CDCl.sub.3) δ 200.1, 180.5, 121.0, 46.5, 43.4, 41.5, 37.2, 32.8, 32.3, 28.6, 27.3, 22.9, 19.4, 14.4, 14.3; HRMS (ESI) calcd for C.sub.15H.sub.23O [M+H].sup.+ 219.1743, found 219.1739.

Example 9

Synthesis of (1aR,1bR,6aR)-1,1,2,2-Tetramethyl-1a,1b,2,3,6,6a-hexahydro cyclo propa[a]inden-4(1H)-one (1h)

[0123] ##STR00023##

[0124] IR (film) vmax 1668, 1367, 1246 cm.sup.−1; .sup.1H NMR (400 MHz, CDCl.sub.3) δ 5.78 (s, 1H), 2.71-2.64 (m, 1H), 2.44-2.36 (m, 2H), 2.18 (s, 2H), 1.28-1.26 (m, 1H), 1.13 (s, 3H), 1.11-1.09 (m, 1H), 1.07 (s, 3H), 0.97 (s, 3H), 0.91 (s, 3H); .sup.13C NMR (100 MHz, CDCl.sub.3) δ 199.7, 178.3, 121.4, 53.1, 51.6, 38.5, 32.6, 30.4, 30.0, 28.7, 27.3, 21.8, 19.9, 14.3; HRMS (ESI) calcd for C.sub.14H.sub.21O [M+H].sup.+ 205.1587, found 205.1583.

Example 10

Protocol Adopted for the Mosquito Repellence Bioassay

Methodology

[0125] Mosquito repellence activity was assessed on the basis of protection period (hr) offered by various analogues of Nootkatone against Mosquito bites. The protection period was measured on the basis of the concept “time until the first bite” pioneered by Granette (Comparison of mosquito repellency test under laboratory and field conditions. Granett, P. Proc Ann Meet NJ Mosq Assoc, 1938, vol. 25, 51,). Repellence tests were carried out against 3-5 days old, blood starved but sucrose fed (0.5M solution), Ae. aegypti females mosquitoes, drawn from well-established laboratory colony maintained at 27±1° C. Temperatureand 70±5% Relative humidity. The light intensity was regulated at 300-500 lux for testing against laboratory, colonized Ae. aegypti, a day biting mosquito. Human volunteer's hand covered with polythene disposable gloves was introduced in the cage containing about 200 hungry mosquitoes. Mosquitoes were

allowed to bite on the back of the hand through muslin screen stuck over a small window (2 cm×2 cm) cutout in the polythene bag. Various analogues of Nootkatone were loaded on the muslin cloth screen instead of direct skin application so as to avoid the potential risk involved in the evaluation of natural products of unknown mammalian toxicity. All the test solutions were made in Analar grade Acetone The muslin cloth screen was first treated with the analogue taking two doses @0.25 mg/cm.sup.2 and 0.5 mg/cm.sup.2 and the solvent was evaporated before use. Control muslin screen was treated with solvent alone. After introduction of the hand covered with the polythene glove with the treated muslin screen into the mosquito cage, number of mosquito bites received in subsequent 5 minutes were counted. In the event of no bites in the initial 5 minutes exposure, the test hand was exposed repeatedly after every consecutive ½ hr for 5 minutes test till the time a confirmed bite was received. Number of hours before the receipt of a confirmed bite (Techniques for the evaluation of insect repellents. A critical review (Schreck, C. E, Ann Rev Entomol, 1977, vol 22, 101) represented the protection period offered by the test compound. In control rate of mosquito bite was 10-12 bites/min. Above test were repeated with both male and female human volunteers using different mosquito batches. All the tests were carried out at 27±1° C. temperature between 9.00-17.00 hrs.

Protection Time Offered by Nardoaristolone and its Various Analogues

[0126]

TABLE-US-00001 Protection period in hrs Protection period in hrs @dose @dose Sample 0.25 mg/cm.sup.2 0.50 mg/cm.sup.2 Recemic 3.30 ± 0.0708 5.63 ± 0.1778 nardoaristolone B 1a 4.13 ± 0.0626 5.35 ± 0.0868 1b 1.23 ± 0.040 1.40 ± 0.0549 1c 3.60 ± 0.0791 5.30 ± 0.088 1d 5.64 ± 0.1984 6.29 ± 0.0844 1e 5.22 ± 0.0720 6.46 ± 0.1507 1f 1.53 ± 0.1223 3.48 ± 0.1396 1g  1.8 ± 0.1490 1.99 ± 0.11366 1h 2.19 ± 0.0716 2.38 ± 0.07567 Recemic nootkatone 3.12 ± 0.07 5.06 ± 0.06 Natural nootkatone 5.58 ± 0.171 >7 hrs

ADVANTAGES OF THE INVENTION

[0127] Compounds possess improved efficacy,

[0128] Simple processes for synthesis of natural compounds provided