USE OF ANIMAL ORIGINATED CORNEA MEMBRANE PRODUCTS IN TREATMENT OF EYE AND OUT OF EYE WOUNDS

Abstract

The present disclosure relates to use of animal originated cornea layer in treatment of eye and out of eye wounds. In line with the purpose, the cornea layer is prepared in serum, plate, suspension, cream, and homogenate forms treated with therapeutics.

Claims

1. An animal originated cornea layer for use in a method of treating a wound.

2. A serum comprising the animal originated cornea layer of claim 1.

3. A dressing material comprising the animal originated cornea layer of claim 1.

4. A pharmaceutics composition in cream form comprising the animal originated cornea layer of claim 1.

5. A pharmaceutics composition in suspension form comprising the animal originated cornea layer of claim 1.

6. A homogenate treated with therapeutics comprising the animal originated cornea layer of claim 1.

7. A plate produced by a quick freezing technique and comprising in vivo cells characterized in comprising the animal originated cornea layer of claim 1.

8. A plate produced by slow freezing technique and comprising cells losing vitality characterized in comprising the animal originated cornea layer of claim 1.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0016] In this detailed description, the preferred embodiments of the invention have been described in a manner not forming any restrictive effect and only for purpose of better understanding of the matter.

[0017] The invention relates to use of animal originated cornea in treatment of eye and out of eye wounds. Provided that not limited, examples of such wounding are wet skin surface wounds such as skin, mucosa wounds, surgical cuts, skin ulcers, circulation disorder ulcers, diabetic wounds, cancer related wounds, chemical or burn wounds, tissue and tendon wounding in joints, military, sportive wounding, skin rejuvenation processes, intra oral wounds, sick bed wounds, wounds in anus area, post-surgical wounds and scars, tissue repair requiring tissue deficiencies. Animal sources where cornea can be taken (provided that not limited to those given herein) include bovine, ovine and poultry and pig species.

[0018] For method of preparing said animal originated cornea as wound healer, firstly, eyeball where cornea tissue is taken is provided and procedures of removing cornea from said cornea sphere are realized. After cornea part removed from eyeball is waited in various solutions and flushed and cleaned, it is transformed into desired product form.

[0019] Supply of animal originated cornea tissues is provided from removal of eyeballs under vets control from animal reproduction and slaughtering farms. At this stage, conduct of screening for zoonose (a disease which mess with from an animal to human) and no detection of positive results in terms of particularly Bovine Spongiform (Encephalopathy), Anthrax, tuberculosis and brucellosis are important during production stage and pre-slaughtering. Said obtained eyeballs are kept in preferably with sterile plastic bags and ice blocks ready for processing. During processing of cornea, initially cornea is removed from eyeballs by means of scalpel and flushed by use of balanced saline solution at least three times and tissue particulates and blood remaining in eyeballs are removed. Said balanced saline solution is BSS® Alcon Inc., 6201 South Freeway, Fort Worth., Tex., 76101. Then, corneal buttons taken from eyeball are flushed in antibiotic solution consisting of 50 mcg/ml penicillin, 50 mcg/ml streptomycin, 100 mcg/ml neomycin and 2.5 mcg/ml amphotericin B. Said flushing process can also be made by various chemicals or agents for use of corneal buttons for treatment of various wound types. Thus absorption of various hormones (for instance, growth hormone etc.), polypeptides (for instance insulin), vitamins (for instance Vitamin E a) and some medicines (for instance antibiotics) can be provided.

[0020] Cornea removed from eyeball and flushed is waited on a smooth surface (preferably nitrocellulose filter) in a manner outer surface facing down, inner surface contacting eyeball facing up in order to prevent damage on cornea bottom epithelia preventing formation of undesired oedema. Another material not consisting dye, colouring agents, decolorizing agent, colorizing agent, not affected by freezing and unfreezing operations can also be used instead of said filter surface. In another preferred embodiment of the invention, filter paper wherein no bleaching is used as filter surface and bonded by compressing without use of glue can be used. In line with purpose of storage of said cornea, cornea button is hung preferably onto miniature hangers by latch from sides and thus damage to tissue in form of frame named limbos framing outer surface epithelia, inner surface endothelia and cornea circularly and providing surface healing can be prevented. At this stage, if the prepared cornea plates are to be used fresh in 24 hours, they are prepared on filter paper without waiting in any carrying solution, and can be used. Therefore, a hygiene dressing material ready for use in wound areas is obtained.

[0021] On the other hand, in case a cornea tissue in plate form not containing in vivo cell is desired for another application (for instance, cases where legal obstacles or tissue rejection probability are high for use of in vivo tissue), said cornea particles are prepared in 0.5×0.5 cm, 0.5×1 cm, 1×2 cm, 1×3, 2×2 cm and 3×3 cm sizes and frozen by means of slow freezing technique and solved at room temperature and then drying process is started. Drying process is performed in a vacuum container at 0.2 Torr pressure and for 24 hours. Then treatment with glutaraldehyde solution of 0.25% is performed and kept at room temperature for one hour. Then, it is flushed with buffered phosphate (PBS) in saline solution and glutaraldehyde wastes are removed. Lastly, dry frozen plates are sterilized by ethylene oxide and prepared in plate product ready for applying onto wound.

[0022] Particularly, when cornea tissue in powder form not containing in vivo cell is desired, cornea particulates are frozen in storage solution by slow freezing technique. After unfreezing at room temperature, tissues are dried, crushed in sterile crushing tools into powder form and can be prepared in vials of different volumes from 0.25 cc to 6.0 cc (0.25 cc, 0.50 cc, 1.0 cc, 2.0 cc, 4.0 cc, 6.0 cc).

[0023] However, if cornea buttons production in a form wherein cell in vivo is kept is desired, after dehydration process by use of chemicals, tissues are frozen at fluid nitrogen tanks of −80 to −20° C. temperatures by quick freezing techniques and then unfreezing at room temperature before use. Frozen cornea buttons obtained by this method are safe in terms of sterility and can be stored for up to one year and can be shipped long distance.

[0024] In conclusion, animal originated cornea tissue are used as described below; [0025] is brought suspension by crushing in order to administer into parts such as intra joints, subcutaneous sections by injector, [0026] is homogenized in cream base in order to use as dressing material in broad wound areas, [0027] is used as plate or homogenates by absorbing as insulin, vitamin, antibiotics, growing hormone, silver, cortisone therapeutics, [0028] is used in serum form by crushing and cleaned of cells for injecting subcutaneous use by too small needles, [0029] as drop in serum form, [0030] in plate form containing in vivo cells by quick freezing technique, [0031] in plate form containing cells losing vitality by slow freezing technique.