LAMELLAR CORNEA PRESERVATION SOLUTION

Abstract

Disclosed is a lamellar cornea preservation solution, comprising the following components: 200-500 g/L of glycerol, 5-50 g/L of hyaluronic acid, 10-25 g/L chondroitin sulfate, 1-10 g/L of an antioxidant, and 10-40 mmol/L of a buffer salt solution, the pH value being 6.0-8.0. The present disclosure is suitable for the preservation of lamellar cornea materials, maintains the original collagen fiber structure and the transparency of a cornea, and prevents the collagen fibers of the cornea from being damaged due to irradiation sterilization treatments.

Claims

1. A lamellar cornea preservation solution, comprising the following components: 200-500 g/L of glycerol, 5-50 g/L of hyaluronic acid, 10-25 g/L chondroitin sulfate, 1-10 g/L of an antioxidant, and 10-40 mmol/L of a buffer salt solution, a pH value being 6.0-8.0.

2. The lamellar cornea preservation solution of claim 1, wherein the antioxidant is N-acetylcysteine or vitamin C.

3. The lamellar cornea preservation solution of claim 1, wherein the buffer salt solution is phosphate buffer solution, carbonate buffer solution or Hepes buffer solution.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0015] FIG. 1 The mechanism diagram of the present disclosure.

[0016] FIG. 2 The comparison of preservation results between the present disclosure and the traditional glycerol.

DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS

[0017] The following examples of the present disclosure will be specifically described, which is an example of the present disclosure but not a limitation. A engineer in this field can make some non-essential improvements and adjustments to the present disclosure.

[0018] Formulation examples of the lamellar cornea preservation solution of the present disclosure:

TABLE-US-00001 TABLE 1 components buffer solution (phosphate antioxidant buffer (N- solution or example acetylcysteine Hepes num- hyaluronic chondroitin or buffer bers glycerol acid sulfate vitamin C) solution) 1 200 5 25 5 30 2 250 30 20 3 10 3 300 20 10 3 10 4 300 25 15 3 15 5 350 15 15 5 25 6 350 15 20 5 25 7 200 50 15 8 40 8 300 25 20 3 15 9 500 10 10 10 40 10 450 10 15 10 40 11 500 10 10 10 40 12 400 15 15 5 5

[0019] The corneal preservation solution prepared by the present disclosure can improve the preservation and transparent performances of corneal collagen fiber, and can be widely used in medical filed.

[0020] By repeated tests, the cornea is still transparent after being preserved in the preservation solution of the present disclosure at room temperature for 6 months; while being preserved in traditional glycerol, the cornea is subjected to slightly xanthochroia at room temperature for 1 month, and becoming moderate yellow, turning hard, lossing of original form and beingfragile at room temperature for 6 months (FIG. 2). It demonstrates that for the corneal grafts, the present preservation solution can effectively maintain its transparency, and prevent aging.

[0021] The dehydration effect of the present preservation solution can make the corneal thickness maintained to the original physiological level. A fresh porcine cornea was cut by a 500 μm lamellar cornea shaper, then the thickness of the front lamella was measured by a micro thickness measuring instrument. After being divided into two eaqual pieces along the central line, one piece was put into the present preservation solution and the other into the traditional glycerol preservation solution respectively. The thickness of the corneas were measured at different times, and compared to the original thickness of the cornea, to obtain the ratios of thickness changes at different times after being put into the preservation solutions (FIG. 1). It can be observed that the traditional glycerol preservation solution has a certain dehydration effect on the cornea, which requires the cornea to be rehydrated before use in a surgery. However, the glycerol is small molecule which is easily soluble in water, and the corneal grafts may be too hard due to inadequate rehydration and thus affect operation effect since the rehydration time and degree is difficult to control. While excessive rehydration will lead to graft edema and thus affect vision recovery after surgery. The present preservation solution can effectively maintain corneal thickness, thus the corneal grafts can be sutured directly in a surgery.

[0022] Table 1 Coeneal thickness changes after the corneal graft being put into different preservation solutions (Units: mm)

TABLE-US-00002 TABLE 2 Time after being put into Three One week One month months the present 0.95 ± 0.27 0.92 ± 0.31 1.05 ± 0.29 preservation solution traditional glycerol 0.67 ± 0.25 0.65 ± 0.21 0.64 ± 0.33 preservation solution