ANIONICALLY MODIFIED POLYALLYLAMINE DERIVATIVE, USE OF ANIONICALLY MODIFIED POLYALLYLAMINE DERIVATIVE AS MEDICINE, PARTICULARLY FOR PROPYLAXIS AND TREATMENT OF INFECTIONS OF RESPIRATORY TRACT CAUSED BY HUMAN METAPNEUMOVIRUS (HMPV), HUMAN RHINOVIRUSES (HRV), AND INFECTION BY INFLUENZA VIRUS TYPE A (IAV) AND PHARMACEUTICAL COMPOSITION COMPRISING THE ANIONICALLY MODIFIED POLYALLYLAMINE DERIVATIVE

Abstract

The subject of the invention is an N-sulfonic polyallylamine derivative (NSPAH) with Formula 1, wherein R is —SO.sub.3.sup.− or —H, and n is an integer from 150 to 15000; an application of the N-sulfonic polyallylamine derivative as a medicine, particularly for prevention and treatment of respiratory tract infections caused by the human metapneumovirus (hMPV), respiratory tract infections caused by the human rhinoviruses (HRV), and infections caused by the influenza A virus; as well as a pharmaceutical composition comprising the N-sulfonic polyallylamine derivative and application thereof.

Claims

1. An anionically modified polyallylamine derivative (NSPAH) with Formula 1 ##STR00005## wherein each R is independently selected from such groups as —SO.sub.3.sup.− and —H, and at least one R is —SO.sub.3.sup.− group, and n is an integer from 150 to 15000.

2. The anionically modified polyallylamine derivative according to claim 1, having the form of a sodium salt.

3. The anionically modified polyallylamine derivative according to claim 1 to be used as a medicine.

4. The anionically modified polyallylamine derivative according to claim 1 to be used in therapy and prevention of infections caused by the human metapneumovirus hMPV, respiratory tract infections caused by the human rhinoviruses (HRV) and infections caused by the influenza A virus.

5. A pharmaceutical composition, characterised in that it contains an anionically modified polyallylamine derivative according to claim 1 as the active substance.

6. The composition according to claim 5 to be used as a medicine.

7. The composition according to claim 5 to be used in therapy and prevention of infections caused by the human metapneumovirus hMPV, respiratory tract infections caused by the human rhinoviruses (HRV) and infections caused by the influenza A virus.

8. The composition according to claim 5, characterised in that it has the form of a solution or an aerosol administered to the upper respiratory tract.

9. An application of the anionically modified polyallylamine derivative according to claim 1 for production of a medicine to be used in therapy and prevention of infections caused by the human metapneumovirus hMPV, respiratory tract infections caused by the human rhinoviruses (HRV) and infections caused by the influenza A virus.

10. The application according to claim 9, wherein the anionically modified polyallylamine derivative according to claim 1 is used for production of a medicine having the form of a solution or an aerosol administered to the upper respiratory tract.

Description

BRIEF DESCRIPTION OF THE DRAWING

[0020] FIG. 1 shows FTIR-ATR spectra of the polyallylamine before (PAH-15-0—solid line) and after modification (NSPAH-15-30, NSPAH-15-95, NSPAH-65-75, NSPAH-65-89—dashed lines).

[0021] FIGS. 2a, 2b, 2c and 2d show the results of a cytotoxicity study of NSPAH-15-30, NSPAH-15-95, NSPAH-65-75 and NSPAH-65-89 polymers, respectively, carried out on the LLC-MK2 cell line by XTT and NR tests.

[0022] FIGS. 3a, 3b, 3c and 3d show the results of a cytotoxicity study of NSPAH-15-30, NSPAH-15-95, NSPAH-65-75 and NSPAH-65-89 polymers, respectively, carried out on the MDCK cell line (Madin-Darby dog kidney cells) by XTT and NR tests.

[0023] FIG. 4 shows the inhibition of replication of the human metapneumovirus (hMPV) by the N-sulfonic polyallylamine derivatives according to the invention, depending on the concentration, the degree of substitution with sulfonic groups and the molecular mass of the studied polymers.

[0024] FIG. 5 shows the inhibition of replication of the human rhinovirus (HRV) by the anionically modified polyallylamine derivatives, depending on the concentration and the molecular mass of the studied polymers.

[0025] FIG. 6 shows the inhibition of replication of the influenza A virus (IAV) by the N-sulfonic polyallylamine derivatives, depending on the concentration, the degree of substitution with sulfonic groups and the molecular mass of the studied polymers.

[0026] The subject of the invention is presented in more detail in the following embodiments:

Example 1

Preparation and Physico-Chemical Characterisation of the Anionic Polyallylamine Derivative (NSPAH)

[0027] The reaction of the polyallylamine modification, shown in Scheme 1, was used earlier for preparation of an N-sulfonic chitosan derivative [6].

##STR00004##

[0028] 0.5 g of polyallylamine hydrochloride (PAH-15) with a molecular mass of Mw ˜15 kDa or 2.45 ml of 20% polyallylamine hydrochloride solution (PAH-65) with a molecular mass of Mw ˜65 kDa were dissolved in 25 ml of distilled water. 1.85 g of sodium carbonate was then added, and the mixture was stirred using a magnetic stirrer for 45 minutes in order to unlock the amine groups. During this time, the mixture was degassed by passing nitrogen through the system and afterwards a proper amount (Table 1) of sulfur trioxide-trimethylamine complex (STTC). The reaction was carried out for 48 hours at 55° C., with the mixture being stirred using a magnetic stirrer. After this time, the reaction mixture was cooled to room temperature and dialysed against water for 7 days. The obtained polymers were isolated from the solutions by freezing in a freeze dryer for 48 hours.

[0029] The structure of the anionically modified polyallylamines is confirmed by FTIR-ATR spectra (FIG. 1). Values of the degree of substitution are collected in Table 1.

[0030] In the FTIR-ATR spectra of the modified polyallylamines, bands at 631-662, 1044-1086 and 1211-1198 cm.sup.−1 occur, characteristic for stretching vibrations of sulfonic moieties present in the modified polyallylamine.

[0031] The degree of substitution (DS) with sulfonic groups was calculated based on the results of elemental analysis. The degree of substitution and zeta potential of the obtained polymers are presented in Table 1.

TABLE-US-00001 TABLE 1 Reaction conditions, composition and physico-chemical characteristics of the synthesised N-sulfonic polyallylamines. zeta potential STTC/amine [mV].sup.b PBS zeta potential groups molar DS buffer pH = [mV].sup.c medium Polymer ratio [%].sup.a 7.4 0% DMEM — — — —  −5.1 ± 0.5 PAH-15-0 — 0 +26.4 ± 1.4  +7.0 ± 0.6 PAH-65-0 — 0  +4.3 ± 0.5 — NSPAH-15-95 5.0 95 −11.5 ± 0.4 −15.2 ± 0.6 NSPAH-56-98 5.0 98 −13.6 ± 2.2 −24.4 ± 1.6 NSPAH-65-89 5.0 89 −16.7 ± 0.8 −30.0 ± 0.7 .sup.aThe degree of substitution with sulfonic groups calculated based on elemental analysis. The DS is a percentage of amine groups substituted with the sulfonic group; therefore, e.g., DS = 30% means that in 30% of amine groups in allylamine units, one H atom was replaced with the SO.sub.3.sup.− moiety. .sup.bpolymer concentration 0.5 mg/ml, temperature 25° C.; average of 5 measurements. .sup.cpolymer concentration 0.5 mg/ml, temperature 25° C.; average of 5 measurements.

Example 2

Cytotoxicity of the Studied Polymers

[0032] The cytotoxicity of anionically modified polyallylamines towards LLCMK2 cells (Macaca mulatta monkey kidney cells) and MDCK cells (dog kidney cells) (FIGS. 2, 3) was examined.

[0033] The cytotoxicity was determined based on two tests. The first test consisted of a colorimetric test based on the ability of mitochondrial enzymes (succinate dehydrogenase) to reduce the XTT dye ((2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) to coloured Formazan salts. Determination of the number and condition of cells may be done based on the existence of a direct dependence between cell viability and the amount of dye formed, calculated from an absorbance measurement at the absorption maximum (450 nm) (FIGS. 2, 3).

[0034] Evaluation of cell viability was also carried out using neutral red (NR). The test is based on the ability of neutral red to pass to the cytoplasm via passive transport. The dye accumulates in the lysosomes of living cells. The percentage of living cells is calculated after a lysis of cells and absorption measurements of the obtained solutions at 540 nm (FIGS. 2, 3).

[0035] In the case of the LLC-MK2 line culture, the cells were grown for 6 days in a medium defined with DMEM without serum, but with an addition of trypsin, a medium containing the studied sulfonic polyallylamines in increasing concentrations. After this time, the cytotoxicity of the studied substances was determined according to the methods described above. Moreover, morphological changes in the cells in the presence of the polymers were observed using a phase contrast microscope. For the concentrations used, i.e. 5000, 2500, 1250 and 625 μg/ml, a cytotoxicity level of 50% was not achieved for any of the studied polymers. In other words, the studied sulfonic polyallylamines are not toxic for the LLC-MK2 cells. FIGS. 2a, 2b, 2c and 2d show the results of a cytotoxicity study of NSPAH-15-30, NSPAH-15-95, NSPAH-65-75 and NSPAH-65-89 polymers, respectively, carried out on the LLC-MK2 cell line by XTT and NR tests. The results obtained from the measurements carried out were consistent with the observations concerning the lack of changes in cell morphology.

[0036] In the case of the MDCK line culture, the cells were incubated for 2 days in a medium defined with DMEM without serum containing the studied N-sulfonic polyallylamine derivatives in increasing concentrations. After this time, the cytotoxicity of the studied substances was determined using the methods described above. Moreover, morphological changes in the cells in the presence of the polymers were observed using a phase contrast microscope. For the concentration range of 625-5000 μg/ml, a cytotoxicity level of 50% was not achieved for any of the studied polymers. Therefore, it was proven that the studied N-sulfonic polyallylamine derivatives are not toxic for MDCK cells.

[0037] FIGS. 3a, 3b, 3c and 3d show the results of a cytotoxicity study of NSPAH-15-30, NSPAH-15-95, NSPAH-65-75 and NSPAH-65-89 polymers, respectively, carried out on the MDCK cell line by XTT and NR tests.

[0038] The results obtained from the measurements carried out were consistent with the observations concerning the lack of changes in cell morphology.

Example 3

[0039] Influence of the Studied Polymers on Inhibition of the Cytopathic Effect Caused by Replication of the Human Metapneumovirus (hMPV)

[0040] Inhibition of replication of the human metapneumovirus by sulfonic polyallylamines was examined. It was observed that the antiviral effect was stronger the higher the degree of substitution of the polyallylamine with sulfonic groups and the higher the molecular mass of the polymer was.

[0041] The experiment was carried out by infecting susceptible cells—the LLC-MK2 line—with the human metapneumovirus in the presence of the polymers in increasing concentrations. The cells were in a medium defined with DMEM without bovine serum, but with an addition of trypsin, for the entire time. After two hours of incubation at 37° C., the non-bound virions were washed out by triple rinsing of the cells with a PBS solution, and solutions of polymers with proper concentrations were then introduced. The infected cells were incubated at 37° C. for 6 days.

[0042] After the assigned incubation time, morphological changes were observed using a phase contrast microscope. Inhibition of IAV and hMPV replication and no cytopathic effect (CPE) were observed with the polymer concentrations presented in Table 2.

TABLE-US-00002 TABLE 2 Values of the polymer concentrations, above which inhibition of IAV replication and no CPE were observed. Minimum polymer concentration with which CPE was not Virus Polymer observed [μg/ml] hMPV NSPAH-15-30 >2000 NSPAH-15-95 1000 NSPAH-65-75 500 NSPAH-65-89 500

Example 4

[0043] Study on the Influence of Sulfonic Polyallylamines on hMPV Replication in LLC-MK2 Cells Using RT-PCR Real-Time Analysis.

[0044] The influence of sulfonic polyallylamines on hMPV replication was examined by a measurement of the number of RNA copies in the medium using real-time RT-qPCR analysis (Reverse Transcription Quantitative Polymerase Chain Reaction). In the study, the LLC-MK2 cells were incubated for 6 days in a medium defined with DMEM without bovine serum, but with an addition of trypsin. The infection was carried out in the presence of the polymer; after 2 hours of incubation of the cells with the virus, the medium was removed, and a fresh medium comprising polymers with proper concentrations was then introduced. The incubation was continued for 6 days. When the incubation was completed, total RNA was isolated from the cell supernatants. After the reverse transcription reaction, cDNA was used as a matrix for the PCR.

[0045] The experiment was carried out using increasing concentrations of polymers. The obtained results are shown in FIG. 4, illustrating the inhibition of replication of the human metapneumovirus (hMPV) by the N-sulfonic polyallylamine derivatives, depending on the concentration, the degree of substitution with sulfonic groups and the molecular mass of the studied polymers. The number of viral RNA copies was expressed as the number of RNA copies in 1 ml of the sample.

[0046] Based on the presented dependencies, the values of concentrations of the N-sulfonic polyallylamines were determined, for which a 50% inhibition of replication of the human metapneumovirus (the so-called IC.sub.50) occurred. These values are gathered together in Table 3. It was observed that sulfonic polyallylamines caused an inhibition of replication of the human metapneumovirus, while in the control samples (without the addition of the polymers), normal replication was found. A dependence of the antiviral effect vs. the degree of substitution with sulfonic groups and the molecular mass of the polyallylamines was proven. The studies carried out indicate that the effect is stronger the higher the molecular mass is and with the higher degree of substitution of the polymer is.

TABLE-US-00003 TABLE 3 Values of IC.sub.50 determined based on the results of real-time PCR analysis (qRT-PCR). Polymer concentration Polymer for IC.sub.50 [μg/ml] PAH-15-0 — PAH-65-0 — NSPAH-15-30 335.2 ± 1.21 NSPAH-15-95 239.0 ± 1.20 NSPAH-65-75  20.2 ± 1.02 NSPAH-65-89  12.9 ± 1.01

Example 5

Influence of the Studied Polymers on Inhibition of the Cytopathic Effect (CPE) Caused by Replication of the Human Rhinovirus (HRV)

[0047] Inhibition of rhinovirus replication by anionically modified polyallylamines with molecular masses of 15 kDa, 56 kDa and 65 kDa, and a high degree of substitution with sulfonic groups, 95%, 98% and 89%, respectively, was examined.

[0048] The experiment was carried out by infecting susceptible cells—the HeLa line—with the rhinovirus in the presence of the polymers in increasing concentrations. The cells were in a medium defined with DMEM without serum for the entire time. After two hours of incubation at 32° C., the non-bound virions were washed out by triple rinsing of the cells with a PBS solution, and solutions of polymers with proper concentrations were then introduced. The infected cells were incubated at 32° C. for 2 days till the CPE occurred as a result of HRV infection.

[0049] After the assigned incubation time, morphological changes were observed using a phase contrast microscope. Inhibition of HRV replication, manifesting itself as a lack of the CPE, was observed in concentrations of the polymers equal to 1.0 mg/ml (Table 4).

TABLE-US-00004 TABLE 4 Values of the polymer concentrations, above which inhibition of HVA replication, manifesting itself as a lack of the CPE, was observed. Polymer concentration, above which the CPE was Virus Polymer not observed [mg/ml] HRV NSPAH-15-95 1.0 NSPAH-56-98 1.0 NSPAH-65-89 1.0

Example 6

Study on the Influence of Anionically Modified Polyallylamines on HRV Replication in HeLa Cells Using RT-qPCR Real-Time Analysis.

[0050] The influence of anionically modified polyallylamines on HRV replication was examined by a measurement of the number of RNA copies in the medium using real-time RT-qPCR analysis (Reverse Transcription Quantitative Polymerase Chain Reaction). In the study, the HeLa cells were incubated for 2 days in a medium defined with DMEM without bovine serum. The cells were infected with the HRV at a dose of TCID.sub.50=400 in the presence of the polymers. After 2 hours of incubation at 32° C., the medium was removed, the cells were rinsed with a PBS solution three times, and a fresh medium comprising polymers with proper concentrations was then introduced. The incubation was continued for 2 days at 32° C., and RNA was then isolated from the cell supernatants. After the reverse transcription reaction, RNA was used as a matrix for the PCR.

[0051] The experiment was carried out using increasing concentrations of polymers. The obtained results are shown in FIG. 5. The decrease in the number of copies of viral RNA in the studied sample in relation to the control sample, LRV (Log Reduction Value), was determined using the following formula:

[00001]$\mathrm{LRV}=-\log .Math.\frac{c_i}{c_0}$

where: [0052] the number of copies of viral RNA [copies/ml] in the studied sample for a given concentration of the studied polymer; [0053] the number of copies of viral RNA [copies/ml] in the control sample, i.e. without the polymer inhibitor.

[0054] FIG. 5 shows the inhibition of replication of the human rhinovirus (HRV) by the anionically modified polyallylamine derivatives, depending on the concentration and the molecular mass of the studied polymers.

[0055] It was observed that sulfonic polyallylamines inhibit replication of the human rhinovirus very strongly (a dose of the polymers at a concentration of 100 μg/ml causes a decrease in the amount of the viral RNA in the sample to a non-measurable low value), while in the control samples (without the addition of the polymers), normal HRV replication was found. Also, a dependence between the molecular mass of the N-sulfonic polyallylamines and their antiviral activity against the HRV was proven. The studies carried out indicate that the effect is stronger the higher the molecular mass of the N-sulfonic polyallylamine derivative is.

Example 7

Influence of the Studied Polymers on the Cytopathic Effect Caused by the Influenza a Virus (IAV).

[0056] Inhibition of replication of the influenza A virus by the N-sulfonic polyallylamine derivatives was examined. It was observed that the antiviral effect was stronger the higher the degree of substitution of the polyallylamine with sulfonic groups was and the higher the molecular mass of the polymer was.

[0057] The experiment was carried out by infecting susceptible cells (MDCK) in the presence of increasing concentrations of the polymers. While infecting the cells with the IAV, they were in a medium defined with DMEM without bovine serum. After two hours of incubation at 37° C., the non-bound virions were washed out by triple rinsing of the cells with a PBS buffer, and solutions of polymers with proper concentrations were then introduced. The infected cells were incubated at 37° C. for 2 days.

[0058] After the assigned incubation time, morphological changes were observed using a phase contrast microscope. Inhibition of IAV replication and a lack of the cytopathic effect were observed already at minimum concentrations of the polymers, which is presented in Table 5.

TABLE-US-00005 TABLE 5 Values of the polymer concentrations, above which inhibition of IAV replication and no CPE were observed. Minimum polymer concentration with which the CPE Polymer vanished [μg/ml] NSPAH-15-30 1000 NSPAH-15-95 500 NSPAH-65-75 250 NSPAH-65-89 250

Example 8

Study on the Influence of N-Sulfonic Polyallylamine Derivatives on IAV Replication in MDCK Cells Using RT-PCR Real-Time Analysis.

[0059] The influence of N-sulfonic polyallylamine derivatives on IAV replication was examined by a measurement of the number of RNA copies in the medium using real-time RT-qPCR analysis (Reverse Transcription Quantitative Polymerase Chain Reaction). In the study, the MDCK cells were incubated for 2 days in a medium defined with DMEM without bovine serum. The infection was carried out in the presence of the polymer; after 2 hours, the medium was removed, and a fresh medium comprising polymers with proper concentrations was then introduced. The incubation was continued for 2 days. When incubation was completed, total RNA was isolated from the cell supernatants. After the reverse transcription reaction, cDNA was used as a matrix for the PCR.

[0060] The experiment was carried out using increasing concentrations of polymers. The obtained results are shown in FIG. 6 illustrating the inhibition of replication of the influenza A virus (IAV) by the N-sulfonic polyallylamine derivatives, depending on the concentration, the degree of substitution with sulfonic groups and the molecular mass of the studied polymers. The number of viral RNA copies was expressed as the number of RNA chains in 1 ml of the sample. Concentrations of the N-sulfonic polyallylamines, with which a 50% inhibition of replication of the influenza A virus (the so-called IC.sub.50) was observed, are gathered in Table 6. It was observed that the N-sulfonic polyallylamine derivatives caused an inhibition of replication of the influenza A virus, while in the control samples (without the addition of the polymers), normal replication was found. A dependence of the antiviral effect vs. the degree of substitution with sulfonic groups and the molecular mass of the N-sulfonic polyallylamine derivatives was proven. The studies carried out indicate that the effect is stronger the higher the molecular mass is and the higher the degree of substitution of the polymer is. Non-modified polyallylamines (PAH-15 and PAH-65) did not exhibit antiviral properties, but at the same time they were highly toxic to MDCK and LLC-MK2 cell lines.

TABLE-US-00006 TABLE 6 Values of IC.sub.50 determined based on the results of real-time PCR analysis (qRT-PCR). Polymer concentration Polymer for IC.sub.50 [μg/ml] PAH-15-0 — PAH-65-0 — NSPAH-15-30 53.4 ± 1.7  NSPAH-15-95 4.5 ± 2.1 NSPAH-65-75 0.5 ± 1.2 NSPAH-65-89 0.6 ± 1.1

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