GLYCOPEPTIDE FOR CONTRAST AGENT TARGETING CANCER CELLS AND CONTRAST AGENT KIT CONTAINING THE SAME

Abstract

The present disclosure relates to a glycopeptide targeting cancer cells and a contrast agent kit containing the same. The glycopeptide is one wherein an azide reporting monosaccharide is bound to a substrate peptide. As the substrate peptide is specifically cleaved by cathepsin B in cancer cells, an azide reporting monosaccharide is expressed onto the cell surface via metabolic glycoengineering, thereby providing a target for action as a contrast agent. Accordingly, because the azide is exposed to the cell surface only by cathepsin B, as it is specifically expressed in cancer cells, in particular in metastatic cancer cells, while it is limitedly expressed in normal cells and is hardly excreted out the cells, the cancer cells can be selectively imaged by an azide-specific contrast agent.

Claims

1. A glycopeptide for a contrast agent targeting cancer cells, wherein an azide reporting monosaccharide is bound to a substrate peptide specifically degraded by cathepsin B.

2. The glycopeptide for a contrast agent targeting cancer cells according to claim 1, wherein a linker is bound between the substrate peptide and the azide reporting monosaccharide.

3. The glycopeptide for a contrast agent targeting cancer cells according to claim 2, wherein the substrate peptide is a peptide comprising 4-30 amino acids comprising an amino acid sequence of SEQ ID NO 1.

4. The glycopeptide for a contrast agent targeting cancer cells according to claim 2, wherein the azide reporting monosaccharide is selected from N-azidoalkyl-D-mannosamine, N-azidoalkyl-D-galactosamine and N-azidoalkyl-D-glucosamine.

5. The glycopeptide for a contrast agent targeting cancer cells according to claim 3, wherein the alkyl is C.sub.1-C.sub.3 alkyl.

6. The glycopeptide for a contrast agent targeting cancer cells according to claim 1, wherein the linker is selected from 6-maleimidocaproyl (MC), maleimidopropanoyl (MP), valine-citrulline (val-cit), alanine-phenylalanine (ala-phe), p-aminobenzyloxycarbonyl (PAB), N-succinimidyl 4-(2-pyridylthio)pentanoate (SPP), N-succinimidyl 4-(N-maleimidomethyl)cyclohexane-1 carboxylate (SMCC) and N-succinimidyl (4-iodo-acetyl)aminobenzoate (SIAB).

7. The glycopeptide for a contrast agent targeting cancer cells according to claim 1, wherein the substrate peptide is cleaved from the glycopeptide for a contrast agent targeting cancer cells by cathepsin B which is specifically expressed in cancer cells and then the azide reporting monosaccharide is expressed onto the cancer cell surface.

8. A contrast agent kit targeting cancer cells, which comprises the glycopeptide for a contrast agent targeting cancer cells according to claim 1 and a cyclooctyne-labeling fluorescent material.

9. The contrast agent kit targeting cancer cells according to claim 7, wherein the cyclooctyne is one or more compound selected from a group consisting of dibenzylcyclooctyne (DBCO), difluorocyclooctyne (DIFO), bicyclononyne (BCN), dibenzoazacyclooctyne (DIBAC), dibenzocyclooctynol (DIBO) and azadibenzocyclooctyne (ADIBO).

10. A contrast agent kit targeting cancer cells, which comprises the glycopeptide for a contrast agent targeting cancer cells according to claim 2 and a cyclooctyne-labeling fluorescent material.

11. A contrast agent kit targeting cancer cells, which comprises the glycopeptide for a contrast agent targeting cancer cells according to claim 3 and a cyclooctyne-labeling fluorescent material.

12. A contrast agent kit targeting cancer cells, which comprises the glycopeptide for a contrast agent targeting cancer cells according to claim 4 and a cyclooctyne-labeling fluorescent material.

13. A contrast agent kit targeting cancer cells, which comprises the glycopeptide for a contrast agent targeting cancer cells according to claim 5 and a cyclooctyne-labeling fluorescent material.

14. A contrast agent kit targeting cancer cells, which comprises the glycopeptide for a contrast agent targeting cancer cells according to claim 6 and a cyclooctyne-labeling fluorescent material.

Description

BRIEF DESCRIPTION OF DRAWINGS

[0023] FIG. 1A schematically shows that a bond between a cathepsin B-specific substrate peptide and a linker (S) is cleaved by cathepsin B (dotted line) and the linker (S), the substrate peptide (KGRR) and Ac.sub.3ManAz are released as a result.

[0024] FIG. 1B schematically shows that Ac.sub.3ManAz is metabolized in cancer cells via glycoengineering, expressed onto the cancer cell surface and labeled by a cyclooctyne-labeling fluorescent material DBCO-Cy5.5 via a bioorthogonal click reaction and that RR-S-Ac.sub.3ManAz expresses azide to cell surface glycan in cancer cells via sialic acid biosynthesis whereas it does not in normal cells.

[0025] FIG. 2A shows the 600 MHz .sup.1H-NMR spectrum of Ac.sub.3ManNAz of Example 1.

[0026] FIG. 2B shows the 600 MHz .sup.1H-NMR spectrum of RR-S-Ac.sub.3ManNAz of Example 1.

[0027] FIG. 3A shows the HPLC spectra of RR-S-Ac.sub.3ManNAz (indicated by *) and Ac.sub.3ManNAz (indicated by **) in a reaction solution containing cathepsin B in Example 2 with time.

[0028] FIG. 3B shows the HPLC spectra of RR-S-Ac.sub.3ManNAz (indicated by *) in a reaction solution without cathepsin B in Example 2 with time.

[0029] FIG. 3C shows the time-dependent fluorescence images of an azide reporting monosaccharide on the surface of HT-29 cells in vitro in Example 2. The images are confocal microscopic images of HT-29 cells treated with RR-S-Ac.sub.3ManNAz or Ac.sub.3ManNAz and then with DBCO-Cy5.

[0030] FIG. 4A shows confocal microscopic images showing fluorescence intensity depending on the concentration of RR-S-Ac.sub.3ManNAz in Example 2.

[0031] FIG. 4B shows cytotoxicity depending on the concentration of RR-S-Ac.sub.3ManNAz in Example 2.

[0032] FIG. 5A shows confocal microscopic images for investigating the expression of azide on the surface of HT-29 cells in Example 3.

[0033] FIG. 5B shows a result of quantifying the fluorescence intensity of DBCO-Cy5 via flow cytometry in Example 3.

[0034] FIG. 5C shows western blot images of test groups in Example 3.

[0035] FIG. 6A shows western blot images showing expression of cathepsin B in HT-29 cells, human-derived dermal fibroblasts (HDF cells), human-derived umbilical vein endothelial cells (HUVEC cells) and rat cardiomyocytes (H9C2 cells) in Example 4.

[0036] FIG. 6B shows the band intensity of the western blot images of FIG. 6A.

[0037] FIG. 6C shows the confocal microscopic images showing expression of azide in test groups in Example 4.

[0038] FIG. 7A shows a near-infrared fluorescence image for targeting azide in cancer tissue into which RR-S-Ac.sub.3ManNAz is injected directly in an HT-29 tumor-bearing animal model in Example 5.

[0039] FIG. 7B shows the near-infrared fluorescence images of major organs in Example 5.

[0040] FIG. 7C shows a result of quantifying the fluorescence intensity in the image of FIG. 7A.

[0041] FIG. 7D shows a result of immunofluorescence staining cathepsin B and azide present in cancer tissue in Example 5.

[0042] FIG. 7E shows a result of quantifying an azide reporting monosaccharide produced on the surface of HT-29 cancer tissue via western blot. Coomassie staining visualizes the total amount of proteins.

[0043] FIG. 8A shows near-infrared fluorescence images for targeting azide in an HT-29 tumor-bearing animal model via intravenous injection of RR-S-Ac.sub.3ManNAz in Example 6.

[0044] FIG. 8B shows fluorescence images showing expression of azide in cancer tissue in Example 6.

[0045] FIG. 8C shows the fluorescence intensity of the image of FIG. 8B.

DETAILED DESCRIPTION OF EMBODIMENTS

[0046] Hereinafter, the present disclosure is described in more detail.

[0047] A glycopeptide for a contrast agent targeting cancer cells of the present disclosure is one wherein an azide reporting monosaccharide is bound to a substrate peptide specifically degraded by cathepsin B.

[0048] The glycopeptide for a contrast agent targeting cancer cells is a substrate peptide which has an amino acid sequence specifically degraded by cathepsin B.

[0049] Because cathepsin B is specifically expressed in cancer cells, in particular in metastatic cancer cells, whereas it is limitedly expressed in normal cells, it can be used to detect metastatic cancer. In addition, because it is hardly excreted extracellularly, the risk of a false positive error of detecting normal cells or normal tissue as cancer cells or cancer tissue is very low. In contrast, a prostate-specific antigen (PSA) protease used by Bertozzi et al., for example, is expressed only in prostate cancer, and thus it is not suitable for detection of cancers other than prostate cancer and it is difficult to detect metastatic cancer. In particular, because the enzyme is excreted extracellularly, there is a risk of a false positive error of falsely detecting normal cells or normal tissue.

[0050] The substrate peptide may be a peptide composed of 4-30 amino acids containing an amino acid sequence of SEQ ID NO 1.

[0051] The substrate peptide may be a peptide composed of 4 amino acids of lysine-glycine-arginine-arginine (KGRR) of SEQ ID NO 1 or a peptide composed of 5-30 amino acids wherein 1-26 amino acid(s) is (are) further bound to the N-terminal lysine of the peptide of SEQ ID NO 1. Cathepsin B recognizes the C-terminal arginine-arginine (RR) sequence of the peptide of SEQ ID NO 1 and cleaves the C-terminal. Accordingly, the amino acid(s) further bound to the N-terminal is(are) not limited in kind or sequence. But, if the number of the amino acids of the substrate peptide exceeds 30, glycoengineering may be restricted.

[0052] Specifically, the amino group of the N-terminal amino acid of the substrate peptide may be acetylated.

[0053] During the preparation procedure of the glycopeptide for a contrast agent targeting cancer cells, an amine protecting group may be used to protect the nitrogen atom of the amine group of the substrate peptide. The amine protecting group may be any one known in the art without limitation. For example, a methyloxycarbonyl group, a benzyloxycarbonyl group, a p-methoxybenzyloxycarbonyl group, a t-butyloxycarbonyl (Boc) group, a 9-fluorenylmethyloxycarbonyl (FMOC) group, an allyloxycarbonyl (Alloc) group, a benzoyl (Bz) group, a benzyl (Bn) group, a p-methoxybenzyl (PMB) group, a 3,4-dimethoxybenzyl (DMPM) group, a p-methoxyphenyl (PMP) group, a tosyl (Ts) group, a trimethylsilylethyloxycarbonyl (Teoc) group, a benzhydryl group, a triphenylmethyl (trityl) group, a (4-methoxyphenyl)diphenylmethyl (Mmt) group, a dimethoxytrityl (DMT) group, a diphenylphosphino group, etc. may be used.

[0054] The azide reporting monosaccharide (or azide reporter) expresses azide onto the surface of cancer cells via glycoengineering and may be one or more selected from N-azidoalkyl-D-mannosamine, N-azidoalkyl-D-galactosamine and N-azidoalkyl-D-glucosamine. In the azide reporting monosaccharide, the alkyl group may be a C.sub.1-C.sub.3 alkyl group, specifically an acetyl group.

[0055] The substrate peptide and the azide reporting monosaccharide may be bound directly or a linker may be bound between them. When bound to the substrate peptide, the linker maintains a stable bonding state with the azide reporting monosaccharide under in-vivo physiological environment. But, when the C-terminal of the substrate peptide is cleaved by cathepsin B, the azide reporting monosaccharide is separated from the linker and released intact. Accordingly, the linker does not affect the expression of azide onto the cancer cell surface via glycoengineering.

[0056] One end of the linker may be bound to the C-terminal of the substrate peptide and the other end may be bound to the C-6 hydroxyl group of the azide reporting monosaccharide. For example, the linker may be selected from 6-maleimidocaproyl (MC), maleimidopropanoyl (MP), valine-citrulline (val-cit), alanine-phenylalanine (ala-phe), p-aminobenzyloxycarbonyl (PAB), N-succinimidyl 4-(2-pyridylthio)pentanoate (SPP), N-succinimidyl 4-(N-maleimidomethyl)cyclohexane-1 carboxylate (SMCC) and N-succinimidyl (4-iodo-acetyl)aminobenzoate (SIAB).

[0057] Therefore, when the glycopeptide for a contrast agent targeting cancer cells is administered into the body, glycoengineering does not occur in normal cells or normal tissue because the substrate peptide is not cleaved and, accordingly, azide is not expressed onto the surface of normal cells. In contrast, in cancer cells or cancer tissue, cleavage occurs between the substrate peptide and the azide reporting monosaccharide, between the substrate peptide and the linker or between the linker and the azide reporting monosaccharide by cathepsin B expressed in the cancer cells. As a result, the azide reporting monosaccharide is exposed onto the cancer cell surface via glycoengineering and azide is expressed.

[0058] Because the azide is expressed selectively on the cancer cell surface, cancer cells can be detected selectively by administering a cyclooctyne-labeling fluorescent material which binds specifically to the azide into the body. The glycopeptide for a contrast agent targeting cancer cells is particularly suitable to detect metastatic cancer.

[0059] FIG. 1 schematically shows that Ac.sub.3ManAz is released onto the surface of cancer cells from RR-S-Ac.sub.3ManAz, a glycopeptide for a contrast agent targeting cancer cells according to an exemplary embodiment of the present disclosure, by cathepsin B.

[0060] FIG. 1A schematically shows that a bond between a cathepsin B-specific substrate peptide and a linker (S) is cleaved by cathepsin B (dotted line) and the linker (S), the substrate peptide (KGRR) and Ac.sub.3ManAz are released as a result.

[0061] FIG. 1B schematically shows that Ac.sub.3ManAz is metabolized in cancer cells via glycoengineering, expressed onto the cancer cell surface and labeled by a cyclooctyne-labeling fluorescent material DBCO-Cy5.5 via a bioorthogonal click reaction and that RR-S-Ac.sub.3ManAz expresses azide to cell surface glycan in cancer cells via sialic acid biosynthesis whereas it does not in normal cells.

[0062] In the cyclooctyne-labeling fluorescent material, the cyclooctyne may be one or more compound selected from a group consisting of dibenzylcyclooctyne (DBCO), difluorocyclooctyne (DIFO), bicyclononyne (BCN), dibenzoazacyclooctyne (DIBAC), dibenzocyclooctynol (DIBO) and azadibenzocyclooctyne (ADIBO).

[0063] The fluorescent material may be, for example, cyanine, allophycocyanin, fluorescein, tetramethylrhodamine, BODIPY, Alexa, etc. Among these fluorescent materials, cyanine dyes are preferred because they emit and absorb near-infrared light and thus exhibit low interference in cells, blood, biological tissues, etc. Specifically, Cy5.5 may be used.

[0064] The cyclooctyne-labeling near-infrared fluorescent material allows for in-vivo imaging of tissues of autoimmune disease patients by irradiating near-infrared light and various types of molecular imaging are possible when radioisotopes, quantum dots and MRI contrast agents are introduced together.

[0065] Hereinafter, the present disclosure will be described in more detail through examples. However, the following examples are for illustrative purposes only and not intended to limit the scope of this disclosure.

Example 1: Synthesis of RR-S-Ac3ManNAz

[0066] 4-Aminobenzyl alcohol (227 mg, 1.8 mmol) was added to a solution of Ac-K(Alloc)GR(Alloc).sub.2R(Alloc).sub.2-OH (901 mg, 0.92 mmol) and N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ; 455 mg, 1.8 mmol) in anhydrous dimethylformamide (DMF; 25 mL). The resulting solution was stirred at room temperature for 24 hours under argon gas. After removing DMF from the solution under reduced pressure, the product was precipitated in diethyl ether and washed 3 times with diethyl ether (20 mL) to yield Ac-K(Alloc)GR(Alloc).sub.2R(Alloc).sub.2-PABOH [mass (ESI-MS, MW 1083.15): m/z 1084.3 [M+H].sup.+].

[0067] Ac-K(Alloc)GR(Alloc).sub.2R(Alloc).sub.2-PABOH (145 mg, 0.13 mmol) and 4-nitrophenyl chloroformate (4-NPC, 32.4 mg, 0.16 mmol) were dissolved in anhydrous dichloromethane (DOM; 3.5 mL) under argon gas and 2,6-lutidine (47 μL, 0.40 mmol) was added. The resulting solution was stirred for 6 hours while further adding DMF (1.5 mL), 2,6-lutidine (74 μL, 0.64 mmol) and 4-nitrophenyl chloroformate (27 mg, 0.13 mmol). The reaction solution was extracted with ethyl acetate and then purified by preparative high-performance liquid chromatography (HPLC) to yield Ac-K(Alloc)GR(Alloc).sub.2R(Alloc).sub.2-PABC [mass (ESI-MS, MW 1248.25): m/z 1249.6 [M+H].sup.+].

[0068] Ac.sub.3ManNAz (21.5 mg, 0.055 mmol) and N,N-dimethylpyridin-4-amine (DMAP; 12.3 mg, 0.1 mmol) were added to a solution of Ac-K(Alloc)GR (Alloc).sub.2R(Alloc).sub.2-PABC (63 mg, 0.05 mmol) in anhydrous DCM (5 mL) and stirred overnight at room temperature under argon gas. The volatile components were removed under reduced pressure and the residue was purified by preparative HPLC to yield Ac-K(Alloc)GR(Alloc).sub.2R(Alloc).sub.2-PABC-Ac.sub.3ManNAz [mass (ESI-MS, MW 1497.48): m/z 1498.1 [M+H].sup.+].

[0069] In order to remove the amine protecting group from the peptide, Ac-K(Alloc)GR(Alloc).sub.2R(Alloc).sub.2-PABC-Ac.sub.3ManNAz (34.7 mg, 0.023 mmol), tetrakis(triphenylphosphine)palladium (13.4 mg, 0.0116 mmol), tributyltin hydride (106 μL, 0.40 mmol) and acetic acid (26.6 μL, 0.464 mmol) were dissolved in anhydrous DMF (3 mL) and stirred at room temperature for 1 hour under argon gas. The residue was purified by preparative HPLC to yield RR-S-Ac.sub.3ManNAz [mass (ESI-MS, MW 1077.1): m/z 1078.1 [M+H].sup.+].

[0070] To confirm the chemical structures of Ac.sub.3ManNAz and RR-S-Ac.sub.3ManNAz, they were dissolved respectively in DMSO-d.sub.6 and characteristic peaks were measured by 600 MHz .sup.1H-NMR (DD2 600 MHz FT NMR, Agilent Technologies, USA). The result is shown in FIGS. 2A and 2B. The characteristic .sup.1H-NMR peaks of Ac.sub.3ManNAz and RR-S-Ac.sub.3ManNAz are shown in FIG. 2A and FIG. 2B, respectively.

Example 2: Time-Dependent In Vitro Release of Ac.SUB.3.ManNAz

[0071] To observe the release of Ac.sub.3ManNAz from RR-S-Ac.sub.3ManNAz in the presence or absence of cathepsin B, 500 μM RR-S-Ac.sub.3ManNAz in a 25 mM 2-(N-morpholine)-ethanesulfonic acid reaction buffer was incubated with or without cathepsin B (50 μg/mL) for 0, 3, 6 and 12 hours at 37° C. The samples were analyzed by reversed-phase HPLC (Agilent Technologies 1200 series, Agilent Technologies, USA) using a C18 analytical column (100:0 H.sub.2O:acetonitrile+0.1% TFA to 60:40 H.sub.2O:acetonitrile+0.1% TFA for 60 minutes). Detection was made at 210 nm. The result is shown in FIG. 3A and FIG. 3B.

[0072] FIGS. 3A and 3B show the HPLC peaks of RR-S-Ac.sub.3ManNAz (indicated by *) and Ac.sub.3ManNAz (indicated by **) in a reaction solution containing cathepsin B (a) and in in a reaction solution not containing cathepsin B (b) with time.

[0073] When RR-S-Ac.sub.3ManNAz was incubated with cathepsin B, the KGRR substrate peptide and Ac.sub.3ManNAz were released from RR-S-Ac.sub.3ManNAz in a time-dependent manner (FIG. 3A). However, in the reaction solution not containing cathepsin B, the release of Ac.sub.3ManNAz could not be observed until 12 hours (FIG. 3B).

[0074] FIG. 3C shows the time-dependent fluorescence images of an azide reporting monosaccharide on the surface of HT-29 cells in vitro. The images are confocal microscopic images of HT-29 cells treated with RR-S-Ac.sub.3ManNAz (5 μM) or Ac.sub.3ManNAz (5 μM) and then with DBCO-Cy5 (200 nM). Red=DBCO-Cy5 channel; blue=DAPI channel.

[0075] In order to confirm the expression of azide on the cancer cell surface, 2.0×10.sup.4 human-derived colon cancer HT-29 cells were seeded into 35-mm cover glass bottom dishes and incubated with media containing RR-S-Ac.sub.3ManNAz (5 μM) or Ac.sub.3ManNAz (5 μM) for 24 hours, 48 hours, 72 hours, 96 hours and 120 hours at 37° C. in a carbon dioxide incubator.

[0076] To visualize the expression of the azide reporting monosaccharide on the surface of the HT-29 cells, the cells were incubated with 5 μM DBCO-Cy5 for 2 hours at 37° C. and then washed with Dulbecco's phosphate-buffered saline (DPBS). After treating with a fixation solution for 15 minutes, the nuclei of the cells were stained by treating with a 4,6-diamidino-2-phenylindole (DAPI) solution for 10 minutes. Then, the fluorescence by DBCO-Cy5 bound to the expressed azide was observed using a confocal microscope.

[0077] Both the RR-S-Ac.sub.3ManNAz- and Ac.sub.3ManNAz-treated HT-29 cells showed strong fluorescence signals on the cell surface at 24 hours. For RR-S-Ac.sub.3ManNAz, the fluorescence intensity increased gradually up to 72 hours and then decreased. It is because the HT-29 cells express cathepsin B and, thus, Ac.sub.3ManNAz is released continuously as the substrate peptide is cleaved from RR-S-Ac.sub.3ManNAz. In contrast, the Ac.sub.3ManNAz-treated HT-29 cells showed gradually decreased azide expression after 48 hours.

[0078] FIG. 4A shows confocal microscopic images showing fluorescence intensity depending on the concentration of RR-S-Ac.sub.3ManNAz and FIG. 4B shows cytotoxicity depending on the concentration of RR-S-Ac.sub.3ManNAz.

[0079] As can be seen from FIG. 4A and FIG. 4B, the amount of the azide reporting monosaccharide expressed onto the surface increased with the concentration of RR-S-Ac.sub.3ManNAz in a concentration-dependent manner and cytotoxicity was not observed up to 20 μM. Based on this result, the concentration of RR-S-Ac.sub.3ManNAz was fixed at 5 μM and the expression of the azide reporting monosaccharide was determined after incubation for 72 hours in the following experiments.

Example 3: Evaluation of Azide Expression Efficiency in Cancer Cells by RR-S-Ac3ManNAz Using Cathepsin B Inhibitor

[0080] 2.0×10.sup.4 HT-29 cells were seeded into 35-mm cover glass bottom dishes, incubated for 24 hours at 37° C. in a carbon dioxide incubator, treated with a cathepsin B inhibitor (50 μg/L) and then stabilized for 24 hours. Then, the HT-29 cells were incubated with RR-S-Ac.sub.3ManNAz (5 μM) or Ac.sub.3ManNAz (5 μM) for 72 hours at 37° C. in a carbon dioxide incubator and the fluorescence of DBCO-Cy5 bound to the expressed azide was observed using a confocal microscope in the same manner as in Example 2.

[0081] FIG. 5A shows the confocal microscopic images confirming that the expression of azide on the surface of the HT-29 cells occurs in a cathepsin B-specific manner. The HT-29 cells treated with RR-S-Ac.sub.3ManNAz showed significant decrease in the fluorescence intensity by DBCO-Cy5 in the presence of the cathepsin B inhibitor. In contrast, the fluorescence intensity of the Ac.sub.3ManNAz-treated HT-29 cells was not affected by the cathepsin B inhibitor.

[0082] The incubated cells were treated with trypsin-EDTA and then with 5 μM DBCO-Cy5 for 2 hours. Then, after washing 3 times with DPBS using a centrifuge, the cells were dispersed in DPBS (2% FBS) and analyzed by flow cytometry.

[0083] As seen from FIG. 5B, the mean fluorescence intensities (MFIs) of the RR-S-Ac.sub.3ManNAz-treated HT-29 cells was 10.16-fold higher when the cathepsin B inhibitor was not added than those of the cathepsin B inhibitor-treated cells. In contrast, the Ac.sub.3ManNAz-treated HT-29 cells showed no change in fluorescence intensity in the presence of the cathepsin B inhibitor although the shift of peaks could be observed.

[0084] The incubated cells were lysed by treating with trypsin-EDTA and then incubated with 500 nM phosphine-PEG-biotin for 6 hours. Each sample was electrophoresed on 10% SDS-PAGE gel at 80 V for 2 hours and 30 minutes and then transferred at 120 V for 1 hour and 30 minutes. After blocking in a blocking solution (BSA 75 mg TBS-T 15 mL) for 1 hour, the sample was incubated with streptavidin-HRP (10000:1) at 4° C. for 24 hours.

[0085] As can be seen from the western blot result of FIG. 5C, the cells treated with RR-S-Ac.sub.3ManNAz or Ac.sub.3ManNAz showed strong band intensities as compared to the HT-29 cells not treated with RR-S-Ac.sub.3ManNAz or Ac.sub.3ManNAz. In particular, it was confirmed that the treatment with the cathepsin B inhibitor leads to inhibited expression of the azide reporting monosaccharide from the glycopeptide. This strongly suggests that the azide reporting monosaccharide can be expressed onto the cancer cell surface only when Ac.sub.3ManNAz is released by being cleaved from RR-S-Ac.sub.3ManNAz by cathepsin B.

Example 4: Evaluation of Azide Expression Efficiency in Normal Cells by RR-S-Ac3ManNAz

[0086] In order to investigate whether the substrate peptide is cleaved from RR-S-Ac.sub.3ManNAz by cathepsin B and azide is expressed onto the cell surface also in normal cells such as human-derived dermal fibroblasts (HDF cells), human-derived umbilical vein endothelial cells (HUVEC cells) and rat cardiomyocytes (H9C2 cells), as in the HT-29 cancer cells, 2.0×10.sup.4 HT-29 cells, human-derived dermal fibroblasts (HDF), human-derived umbilical vein endothelial cells (HUVEC) and rat cardiomyocytes (H9C2) were seeded onto 35-mm cover glass bottom dishes and incubated with RR-S-Ac.sub.3ManNAz (5 μM) or Ac.sub.3ManNAz (5 μM) for 72 hours at 37° C. in a carbon dioxide incubator. Then, the fluorescence of DBCO-Cy5 bound to the expressed azide was observed using a confocal microscope in the same manner as in Example 2.

[0087] As can be seen from FIG. 6A and FIG. 6B, the cathepsin B expression level was significantly lower in the normal cells such as the HDF cells, the HUVEC cells and the H9C2 cells. The relative band intensity showed that the cathepsin B expression level was 2.3-200 folds higher in the HT-29 cells than those of the normal cells.

[0088] Also, as can be seen from FIG. 6C, although the fluorescence intensity was similar for the normal cells and the cancer cells when they were treated with Ac.sub.3ManNAz, azide was expressed only on the surface of the HT-29 cells when they were treated with RR-S-Ac.sub.3ManNAz.

Example 5: Expression of Azide in Cancer Tissue Via Intratumoral Injection of RR-S-Ac3ManNAz in Tumor-Bearing Animal Model

[0089] To prepare a tumor-bearing animal model, 1×10.sup.7 HT-29 cells were injected into both flanks of 5-week-old male nude mice. When tumors grew to 200-250 mm.sup.3 in volume after 5 weeks, 2 mg/kg of a cathepsin B inhibitor (Z-Phe-Ala fluoromethyl ketone, CAS 197855-65-5) administered 4 times into the left cancer tissue of the tumor-bearing animal model via intratumoral injection 24 hours before treatment with RR-S-Ac.sub.3ManNAz. Then, 4 mg/kg of RR-S-Ac.sub.3ManNAz was intratumorally into tumors of both sides for 4 days. To observe azide expressed in the cancer tissue, 100 μg of azadibenzocyclooctyne-Cy5.5 (DBCO-Cy5.5) (200 μL) was administered intravenously and near-infrared fluorescence imaging was conducted 24 hours later. Then, major organs were excised and fluorescence from the organs was detected.

[0090] In order to observe the fluorescence from the cancer tissue, frozen sections of the cancer tissue were prepared and observed using a confocal microscope.

[0091] FIGS. 7A to 7E show the near-infrared fluorescence image of azide in the HT-29 tumor-bearing animal model, the fluorescence images of major organs obtained using a confocal microscope, the result of quantifying the azide expression in the cancer tissue, the fluorescence images of the cancer tissue and the result of western blot analysis.

[0092] As can be seen from FIG. 7A, the tumor-bearing animal model showed significant difference in the fluorescence intensity in the left and right flanks. RR-S-Ac.sub.3ManNAz could effectively detect cancer not only in the cell level but also in the tumor-bearing animal model by targeting the azide.

[0093] It was also confirmed that the fluorescence intensity of the left and right flanks of FIG. 7A was higher than that of the major organs of FIG. 7B. This suggests that RR-S-Ac.sub.3ManNAz generates the azide reporting monosaccharide on the surface of the cancer tissue and the azide reporting monosaccharide is chemically labeled with DBCO-Cy5.5 via a bioorthogonal click reaction.

[0094] For more precise analysis, fluorescence molecular topography was conducted for the cancer tissue and the result is shown in FIG. 7C. The fluorescence intensity of DBCO-Cy5.5 in the right cancer tissue was 2.97 folds higher than the left cancer tissue.

[0095] In addition, the right cancer tissue treated with RR-S-Ac.sub.3ManNAz showed stronger red fluorescence of DBCO-Cy5.5 than the left cancer tissue treated with the cathepsin B inhibitor and RR-S-Ac.sub.3ManNAz (see FIG. 7D). This suggests that the azide reporting monosaccharide was not generated on the surface of the left cancer tissue due to the cathepsin B inhibitor.

[0096] A result of analyzing the amount of the azide reporting monosaccharide via western blot to quantify the azide groups generated on the surface of the HT-29 cancer tissue is shown in FIG. 7E. A significantly stronger band intensity could be confirmed when RR-S-Ac.sub.3ManNAz was injected into the cancer tissue as compared to when physiological saline or the cathepsin B inhibitor was injected.

Example 6: Expression of Azide in Cancer Tissue Via Intravenous Injection of RR-S-Ac3ManNAz in Tumor-Bearing Animal Model

[0097] To prepare a tumor-bearing animal model, 1×10.sup.7 HT-29 cells were injected into the left flank of 5-week-old male nude mice. When tumors grew to 200-250 mm.sup.3 in volume after 5 weeks, 32 mg/kg of RR-S-Ac.sub.3ManNAz was injected into the tail vein of the tumor-bearing animal model for 3 days. In order to target the azide expressed in the cancer tissue, 100 μg of azadibenzocyclooctyne-Cy5.5 (DBCO-Cy5.5) (200 μL) was injected intravenously and near-infrared fluorescence imaging was conducted 24 hours later.

[0098] As can be seen from FIG. 8A, a significant difference in fluorescence intensity was observed in the left flank of the tumor-bearing animal model. Accordingly, it was confirmed that RR-S-Ac.sub.3ManNAz can express azide specifically in cancer tissue when it is injected intravenously, as well as intratumorally, and cancer can be effectively detected by targeting the azide expressed in the cancer tissue.

[0099] Also, as can be seen from FIG. 8B and FIG. 8C, it was confirmed that the fluorescence intensity was higher also in the cancer tissue of the animals to which RR-S-Ac.sub.3ManNAz was injected intravenously. This suggests that RR-S-Ac.sub.3ManNAz generates an azide reporting monosaccharide on the cancer tissue and the azide reporting monosaccharide is chemically labeled with DBCO-Cy5.5 via a bioorthogonal click reaction.