DETECTION DEVICE AND METHOD FOR SIMULTANEOUS IN-SITU MEASUREMENT OF DISSOLVED OXYGEN AT DIFFERENT SUBMERGED PLANT LEAF-WATER INTERFACE LEVELS

Abstract

A detection device for simultaneous in-situ measurement of dissolved oxygen at different submerged plant leaf-water interface levels. The detection device includes a dissolved oxygen micro-optrode host. A plurality of detection probes are externally connected to the dissolved oxygen micro-optrode host and can extend out probes. The detection device includes a leaf clamp having an upper clamping head, a lower clamping head, a hinged shaft and a clamping handle. Each of the upper and lower clamping head includes a plurality of water passing cavities penetrating through the back and the front thereof. Each of the upper and lower clamping head includes a plurality of slots horizontally extending inwardly of the corresponding clamping head. The detection device includes a plurality of insertion pieces having a probe groove. The detection probe is locatable in the respective probe groove for fixation. The insertion pieces are insertable and fixable in the slots.

Claims

1. A detection device for simultaneous in-situ measurement of dissolved oxygen at different submerged plant leaf-water interface levels, comprising a dissolved oxygen micro-optrode host, wherein a plurality of detection probes are externally connected to the dissolved oxygen micro-optrode host and can extend out probes used for detecting dissolved oxygen, and characterized in that the detection device further comprises a leaf clamp, and the leaf clamp consists of an upper clamping head, a lower clamping head, a hinged shaft and a clamping handle; submerged plant leaves are clamped between the upper clamping head and the lower clamping head, each of the upper clamping head and the lower clamping head is provided with a plurality of water passing cavities penetrating through the back and the front thereof, and each of the upper clamping head and the lower clamping head is further provided with a plurality of slots horizontally extending inwardly of the corresponding clamping head; the slots are communicated with each water passing cavity; and the detection device further comprises a plurality of insertion pieces, wherein each of the insertion pieces is provided with a probe groove, the detection probe can be placed in the respective probe groove for fixation, the insertion pieces can be inserted and fixed in the slots, and the probes extending out can be positioned in different water passing cavities by adjusting the depths of the insertion pieces into the slots.

2. The detection device for simultaneous in-situ measurement of dissolved oxygen at different submerged plant leaf-water interface levels according to claim 1, wherein openings of the slots are positioned at the front end surfaces of the upper clamping head and the lower clamping head.

3. The detection device for simultaneous in-situ measurement of dissolved oxygen at different submerged plant leaf-water interface levels according to claim 2, wherein the upper clamping head, the lower clamping head and the clamping handle can all rotate around the hinged shaft, and opening and closing of the upper clamping head and the lower clamping head are controlled by pressing the clamping handle around the hinged shaft.

4. The detection device for simultaneous in-situ measurement of dissolved oxygen at different submerged plant leaf-water interface levels according to claim 3, wherein the detection probe comprises a flexible optical fiber and a rigid optical fiber, wherein the rigid optical fiber is the probe and the rear end of the rigid optical fiber is connected to the dissolved oxygen micro-optrode host through the flexible optical fiber.

5. The detection device for simultaneous in-situ measurement of dissolved oxygen at different submerged plant leaf-water interface levels according to claim 4, wherein the detection probe further comprises a probe pushing structure, wherein the probe pushing structure comprises a pushing pipe, a pushing column, a friction block, a seal ring, a pushing handle and a pipe backseat; the pushing handle is connected to the rear end of the pushing column, the front end of the pushing column is extended into the pushing pipe to be fixedly connected to the friction block in the pushing pipe, the friction block is frictionally engaged with the inner wall of the pushing pipe, the pipe backseat blocks off the rear end of the pushing pipe, the friction block is fixedly connected to the rear part of the probe, the seal ring is fixed in the pushing pipe to seal and divide a cavity of the pushing pipe into a front cavity and a rear cavity, the front cavity is provided with a through window through which water can enter the front cavity, and accordingly, the probe groove is provided with a groove window at a position corresponding to the through window, so that the through window is communicated with the water passing cavity when the insertion piece fixing the detection probe is fixed in the slot; and when an external force for propelling the pushing handle is larger than a friction force between the friction block and the pushing pipe, the friction block slides in the pushing pipe, so that the probe extends out into the front cavity.

6. The detection device for simultaneous in-situ measurement of dissolved oxygen at different submerged plant leaf-water interface levels according to claim 5, wherein the flexible optical fiber is fixedly connected to the pushing handle.

7. The detection device for simultaneous in-situ measurement of dissolved oxygen at different submerged plant leaf-water interface levels according to claim 6, wherein a plurality of fixing grooves are formed in the side of the insertion piece; a fixing boss is arranged in the slot; and when the insertion piece is inserted into the slot, the fixing grooves are clamped into the fixing boss, so that the insertion piece is in stationary fit with a detection kit, and the depth of the insertion piece inserted into the slot is adjusted by selecting different fixing grooves to be clamped with the fixing boss.

8. A detection method for simultaneous in-situ measurement of dissolved oxygen at different submerged plant leaf-water interface levels, wherein by comprising the following steps: step 1, assembling a detection device, namely selecting a plurality of detection probes as required, specifying slots, clamping each detection probe into a probe groove of a corresponding insertion piece to ensure that the detection probe and the insertion piece are positioned, and then inserting the insertion pieces into the specified slots; step 2, pushing out a probe of each detection probe into a front cavity of a pushing pipe; step 3, extending a leaf clamp into water, clamping submerged plant leaves between an upper clamping head and a lower clamping head, wherein front and back leaf surfaces of the submerged plant leaves respectively are communicated with the water passing cavity on the upper clamping head and/or lower clamping head, and injecting water into the water passing cavity and the front cavity of the pushing pipe; step 4, collecting dissolved oxygen data in the water by each probe, transmitting the data to a dissolved oxygen micro-optrode host, and displaying after analyzing and processing the data by the dissolved oxygen micro-optrode host.

9. The detection method for simultaneous in-situ measurement of dissolved oxygen at different submerged plant leaf-water interface levels according to claim 8, wherein when all the insertion pieces are inserted into the slots on the upper clamping head or lower clamping head, the detection device will detect the dissolved oxygen at one side of the submerged plant leaf; when the insertion pieces are inserted into the upper clamping head and lower clamping head, the detection device will detect the dissolved oxygen at both the front and back sides of the submerged plant leaf.

10. The detection method for simultaneous in-situ measurement of dissolved oxygen at different submerged plant leaf-water interface levels according to claim 8, wherein by further comprising step 5, namely after detection is completed, lifting the leaf clamp from the water body, and taking out the insertion pieces, wherein the method of taking out the insertion pieces comprises: pulling a pushing handle backwards, so that the friction block slides backwards; when the friction block slides to the rear end of the pushing pipe, the probes are retracted to the rear cavity of the pushing pipe; continuously pulling the pushing handle backwards; the friction block abuts against the pipe backseat, so that the whole insertion piece moves backwards; and the fixing grooves exit from the fixing boss to draw out the insertion piece.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0029] FIG. 1 is a structural schematic view of a leaf clamp;

[0030] FIG. 2 is a left view of FIG. 1;

[0031] FIG. 3 is a structural schematic view of a detection probe;

[0032] FIG. 4 is a structural schematic view of an insertion piece;

[0033] FIG. 5 is a schematic view of a detection probe placed in an insertion piece;

[0034] FIG. 6 is a schematic view of a probe extending outwards;

[0035] FIG. 7 is a schematic view of an insertion piece inserted into a leaf clamp and detection of dissolved oxygen at the front and back sides of a submerged plant leaf;

[0036] FIG. 8 is an enlarged structural view of part A of FIG. 7; and

[0037] FIG. 9 is a structural schematic block diagram of dissolved oxygen micro-optrode.

[0038] The reference numerals are as follows: dissolved oxygen micro-optrode host 1, detection probe 2, probe 21, flexible optical fiber 22, leaf clamp 3, upper clamping head 31, lower clamping head 32, hinged shaft 33, clamping handle 34, water passing cavity 35, slot 36, fixing boss 37, insertion piece 4, probe groove 41, groove window 41a, fixing groove 42, probe pushing structure 5, pushing pipe 51, front cavity 51a, rear cavity 51b, pushing column 52, friction block 53, seal ring 54, pushing handle 55, pipe backseat 56, through window 57, submerged plant leaf 6.

DETAILED DESCRIPTION OF THE INVENTION

[0039] Embodiments of the present invention will be described in further details with reference to the accompanying drawings.

[0040] As shown in FIGS. 1 to 9, the detection device for simultaneous in-situ measurement of dissolved oxygen at different submerged plant leaf-water interface levels of the present invention comprises a dissolved oxygen micro-optrode host 1, and a plurality of detection probes 2 are connected to the dissolved oxygen micro-optrode host 1 and can extend out probes 21 used for detecting dissolved oxygen, wherein the detection device further comprises a leaf clamp 3, and the leaf clamp 3 consists of an upper clamping head 31, a lower clamping head 32, a hinged shaft 33 and a clamping handle 34; submerged plant leaves are clamped between the upper clamping head 31 and the lower clamping head 32, each of the upper clamping head 31 and the lower clamping head 32 is provided with a plurality of water passing cavities 35 penetrating through the back and the front thereof, and each of the upper clamping head 31 and the lower clamping head 32 is further provided with a plurality of slots 36 horizontally extending inwardly of the corresponding clamping head; the slots 36 are communicated with each water passing cavity 35; and the detection device further comprises a plurality of insertion pieces 4, wherein each of the insertion pieces 4 is provided with a probe groove 41, the detection probes 2 can be placed in the respective probe grooves 41 for fixation, and the insertion pieces 4 can be inserted and fixed in the slots 36, and the probes 21 extending out can be positioned in different water passing cavities 35 by adjusting the depths of the insertion pieces 4 into the slots 36.

[0041] In the embodiments, the openings of the slots 36 are positioned at the front end surfaces of the upper clamping head 31 and the lower clamping head 32.

[0042] In the embodiment, the upper clamping head 31, the lower clamping head 32 and the clamping handle 34 can all rotate around the hinged shaft 33, and opening and closing of the upper clamping head 31 and the lower clamping head 32 are controlled by pressing the clamping handle 34 around the hinged shaft 33.

[0043] In the embodiment, the detection probe 2 comprises a flexible optical fiber 22 and a rigid optical fiber, wherein the rigid optical fiber is the probe 21 and the rear end of the rigid optical fiber is connected to the dissolved oxygen micro-optrode host 1 through the flexible optical fiber 22.

[0044] In the embodiment, the detection probe 2 further comprises a probe pushing structure 5, wherein the probe pushing structure 5 comprises a pushing pipe 51, a pushing column 52, a friction block 53, a seal ring 54, a pushing handle 55 and a pipe backseat 56; the pushing handle 55 is connected to the rear end of the pushing column 52, the front end of the pushing column 52 is extended into the pushing pipe 51 to be fixedly connected to the friction block 53 in the pushing pipe 51, the friction block 53 is frictionally engaged with the inner wall of the pushing pipe 51, the pipe backseat 56 blocks off the rear end of the pushing pipe 51; the friction block 53 is fixedly connected to the rear part of the probe 21, the seal ring 54 is fixed in the pushing pipe 51 to seal and divide a cavity of the pushing pipe 51 into a front cavity 51a and a rear cavity 51b, the front cavity 51a is provided with a through window 57, through which water can enter the front cavity 51a, and accordingly, the probe groove 41 is provided with a groove window 41a at a position corresponding to the through window 57, so that the through window 57 is communicated with the corresponding water passing cavity 35 when the insertion piece 4 fixing the detection probe 2 is fixed in the slot 36; and when an external force for propelling the pushing handle 55 is larger than a friction force between the friction block 53 and the pushing pipe 51, the friction block 53 slides in the pushing pipe 51, so that the probe 21 extends out into the front cavity 51a.

[0045] In the embodiment, the flexible optical fiber 22 is fixedly connected to the pushing handle 55.

[0046] In the embodiment, a plurality of fixing grooves 42 are formed in the side of the insertion piece 4, a fixing boss 37 is arranged in the slot 36, when the insertion piece 4 is inserted into the slot 36, the fixing grooves 42 are clamped into the fixing boss 37, so that the insertion piece 4 is in stationary fit with a detection kit 3, and the depth of the insertion piece 4 inserted into the slot 36 is adjusted by selecting different fixing grooves 42 to be clamped with the fixing boss 37.

[0047] A detection method for simultaneous in-situ measurement of dissolved oxygen at different submerged plant leaf-water interface levels comprises the following steps:

[0048] Step 1, assembling a detection device, namely selecting a plurality of detection probes 2 as required, specifying slots 36, clamping each detection probe 2 into a probe groove 41 of a corresponding insertion piece 4 to ensure that the detection probe 2 and the insertion piece 4 are positioned, and then inserting the insertion pieces 4 into the specified slots 36;

[0049] Step 2, pushing out a probe 21 of each detection probe 2 into a front cavity 51a of a pushing pipe 51;

[0050] Step 3, extending a leaf clamp 3 into water, clamping submerged plant leaves between an upper clamping head 31 and a lower clamping head 32, wherein front and back leaf surfaces of the submerged plant leaves respectively are communicated with the water passing cavity 35 on the upper clamping head 31 and/or lower clamping head 32, and injecting water into the water passing cavity 35 and the front cavity 51a of the pushing pipe 51;

[0051] Step 4, collecting dissolved oxygen data in the water by each probe 21, transmitting the data to a dissolved oxygen micro-optrode host 1, and displaying after analyzing and processing the data by the dissolved oxygen micro-optrode host 1;

[0052] Step 5, after detection is completed, lifting the leaf clamp 3 from the water body, and taking out the insertion pieces 4, wherein the method of taking out the insertion pieces 4 comprises: pulling the pushing handle 55 backwards, so that the friction block 53 slides backwards; when the friction block 53 slides to the rear end of the pushing pipe 51, enabling the probes 21 to be retracted to the rear cavity 51b of the pushing pipe 51, continuously pulling the pushing handle 55 backwards; the friction block 53 abuts against the pipe backseat 56, so that the whole insertion piece 4 moves backwards; and the fixing grooves 42 exit from the fixing boss 37 to draw out the insertion piece 4.

[0053] When all the insertion pieces 4 are inserted into the slots 36 on the upper clamping head 31 or lower clamping head 32, the detection device will detect the dissolved oxygen at one side of the submerged plant leaf; when the insertion pieces 4 are inserted into the upper clamping head 31 or lower clamping head 32, the detection device will detect the dissolved oxygen at both the front and back sides of the submerged plant leaf.

[0054] According to the present invention, the height of the leaf clamp 3 does not exceed 1 cm, the thickness of the insertion piece 4 is about 1 mm, and 4-8 inserting pieces 4 can be inserted into one leaf clamp 3.

[0055] The detection probes 2 adopted by the embodiment are fluorescent probes, and the probes detect the dissolved oxygen of the submerged plant leaves according to the following principle: a dissolved oxygen micro-optrode host 1 is used for emitting lasers, the lasers are emitted to the probes 21 through a flexible optical fiber 22, the front ends of the probes 21 are provided with an oxygen fluorescence sensitive material, the lasers are emitted to the oxygen fluorescence sensitive material, and the fluorescence generated by fluorescence quencher effect is fed back to the dissolved oxygen micro-optrode host 1, the dissolved oxygen micro-optrode host 1 converts the flourescent signal into an electric signal, demodulates the electric signal, and compares the demodulated electric signal with a dissolvable oxygen concentration change relation, so that dissolvable oxygen concentration information is obtained. The oxygen fluorescence sensitive material can be selected from various fluorescent sensitive materials in prior, and good materials are ruthenium complexes, such as Ru(dpp).sub.3Cl.sub.2.

[0056] The above are only preferable embodiments of the present invention, the scope of the present invention is not limited to the embodiments, and the technical scheme under the spirit of the present invention belongs to the scope of the present invention. It should be noted that for those of skilled in the art, various modifications and improvements without departing from the principles shall be within the scope of the present invention.