Liposomes for the treatment of textile materials

Abstract

Liposomes with cosmetic and/or dermopharmaceutical ingredients for the care of the skin, scalp and/or hair and their use in washing agents and/or sprays for the treatment of textile materials.

Claims

1. A textile material with adsorbed liposomes of a size smaller than or equal to 200 nm, which contain at least one phospholipid and cosmetic and/or dermopharmaceutical active ingredients and/or adjuvants, the liposomes being bound to cationic copolymers of acrylic acid with dimethyldiallylammonium chloride, each of the cationic copolymers having positive charges which each electrostatically interact with a phosphate group of the phospholipids which form lipid membranes of the liposomes, each of the cationic copolymers being of a particle size of between 600 and 1700 nm, such that the same cationic copolymer interacts with several of the liposomes bound thereto at once and is bound to a surface of the textile material at several points, and wherein the cationic copolymers form a network of polymer chains over a surface of the textile material, in which the liposomes are embedded.

2. The textile material according to claim 1, wherein the phospholipid of the liposomes is a phosphoglyceride.

3. The textile material according to claim 2, wherein the phosphoglyceride is selected from the group consisting of phosphatidylserine, phosphatidylinositol, phosphatidylethanolamine, phosphatidylcholine, phosphatidic acid, phosphatidylglycerol, diphosphatidylglycerol, phosphorylcholine, their fatty acid esters, hydrogenation products and mixtures thereof.

4. The textile material according to claim 1, wherein the liposomes are formed by one or more phospholipids and one or more surfactants.

5. The textile material according to claim 4, wherein the surfactant is selected from the group consisting of nonionic surfactants, amphoteric surfactants, anionic surfactants, cationic surfactants and mixtures thereof.

6. The textile material according to claim 5, wherein the surfactant is an anionic surfactant.

7. The textile material according to claim 1 wherein the cationic copolymer includes polyquaternium-16.

8. The textile material according to claim 1, wherein the liposomes are microfluidized liposomes.

Description

DESCRIPTION OF THE FIGURES

(1) FIG. 1: FIG. 1 shows 5 different textile materials (non-woven viscose, polyamide, tights, cotton and polyester), the quantity of caffeine bound to the textile material as per quantity of caffeine in the bath where the textile material is submerged for 3 bathings wherein the textile material weight/bath ratios are 1/25 (top graph), 1/50 (middle graph) and 1/100 (bottom graph). Caffeine is one of the cosmetic and/or dermopharmaceutical active ingredients contained in the liposomes of example 3.

(2) FIG. 2: FIG. 2 shows the quantity of caffeine bound to the textile material as per quantity of caffeine in the bath where the textile material is submerged for 3 bathings wherein the textile material weight/bath ratios are 1/25 (top graph), 1/50 (middle graph) and 1/100 (bottom graph), for the same textile materials as FIG. 1, after 2 washes of the textile material.

(3) FIG. 3: shows the quantity of caffeine bound to the textile material as per quantity of caffeine in the bath where the textile material is submerged for 3 bathings wherein the textile material weight/bath ratios are 1/25 (top graph), 1/50 (middle graph) and 1/100 (bottom graph), for the same textile materials as FIG. 1, after 4 washes of the textile material.

(4) FIG. 4: FIG. 4 shows images of liposomes from example 2 through transmission electron microscopy (TEM).

(5) FIG. 5: FIG. 5a shows the image of the mica substrate used for atomic force microscopy. FIG. 5b shows the atomic force microscopy image of the liposomes bound to cationic polymers from example 2. The irregularities concern the cationic polymers which form the network and the clearer, flatter and higher zones concern the liposomes bound to these cationic polymers.

DETAILED DESCRIPTION

(6) As required, detailed embodiments of the present invention are disclosed herein; however, it is to be understood that the disclosed embodiments are merely exemplary of the invention that may be embodied in various and alternative forms. The figures are not necessarily to scale; some features may be exaggerated or minimized to show details of particular components. Therefore, specific structural and functional details disclosed herein are not to be interpreted as limiting, but merely as a representative basis for teaching one skilled in the art to variously employ the present invention.

EXAMPLES

(7) General Methodology

(8) All the reagents and solvents are of synthesis quality and are used without any additional treatment.

Example 1

(9) Obtaining liposomes containing Antarcticine bound to cationic polymers of polyquaternium-16

(10) Antarcticine® (phase A), water, Zemea [INCI: 1,3-propanediol] and phenoxyethanol (ingredients B to D) were added to a suitable vessel. When all the previous components had dissolved Centrolex F [INCI: Lecithin] (ingredient E) was slowly added under intense stirring until it was completely dissolved. Afterwards Labrasol [INCI: PEG-8 Caprylic/Capric Glycerides] (ingredient F) was added and was left being stirred for 10-15 minutes to form an emulsion.

(11) TABLE-US-00001 % IN INGREDIENT (INCI Nomenclature) WEIGHT A PSEUDOALTEROMONAS FERMENT EXTRACT 2.5 A PHENOXYETHANOL, METHYLPARABEN, 0.05 BUTYLPARABEN, ETHYLPARABEN, PROPYLPARABEN, ISOBUTYLPARABEN B 1,3-PROPANEDIOL 8.50 C PHENOXYETHANOL 1.70 D AQUA (WATER) q.s.p. 100 E LECITHIN 10.00 F PEG-8 CAPRYLIC/CAPRIC GLYCERIDES 4.00

(12) The sample was passed through a microfluidizer for a cycle at an entrance pressure of 80 bar and 12500 psi on exit. The liposomes obtained were added to Luviquat HMM552 [INCI: Polyquaternium-16] in a cationic liposome:polymer ratio of 1.5:1 under light stirring.

Example 2

(13) Obtaining liposomes containing Decelerine™ bound to cationic polymers of polyquaternium-16

(14) The liposomes were obtained in the same way as in example 1 but instead of Antarcticine®, Decelerine™ (ingredients from phase A) was used and the same ingredients and quantities as in the other phases.

(15) TABLE-US-00002 % IN INGREDIENT (INCI Nomenclature) WEIGHT A GLYCERIN 1.00 A BARBADOS ALOE (ALOE BARBADENSIS) LEAF 1.00 EXTRACT, SODIUM SORBATE, SODIUM BENZOATE A LAURYL ISOQUINOLIUM BROMIDE, 0.50 ISOPROPYL ALCOHOL A PSEUDOALTEROMONAS FERMENT EXTRACT 0.25 A POLYQUATERNIUM-37 0.20 A DISODIUM EDTA 0.18 A ALLANTOIN 0.05 A GLUCOSE 0.005 A CAPRYLYL GLYCOL 0.005 B 1,3-PROPANEDIOL 8.50 C PHENOXYETHANOL 1.70 D AQUA (WATER) q.s.p. 100 E LECITHIN 10.00 F PEG-8 CAPRYLIC/CAPRIC GLYCERIDES 4.00

(16) The liposomes obtained were added to Luviquat HMM552 [INCI: Polyquaternium-16] in a cationic liposome:polymer ratio of 1.5:1 under light stirring.

Example 3

(17) Obtaining liposomes containing Liporeductyl bound to cationic polymers of polyquaternium-16

(18) The liposomes were obtained in the same way as in example 1 but instead of Antarcticine®, Liporeductyl® (ingredients from phase A) was used and the same ingredients and quantities as in the other phases.

(19) TABLE-US-00003 % IN INGREDIENT (INCI Nomenclature) WEIGHT A GLYCERIN 1.00 A BUTCHERBROOM (RUSCUS ACULEATUS) ROOT 0.57 EXTRACT, MALTODEXTRIN, SILICA A LECITHIN 0.83 A CAFFEINE 0.59 A TEA-HYDROIODIDE 0.44 A CARNITINE 0.30 A BUTYLENE GLYCOL, WATER (AQUA), IVY 0.38 (HEDERA HELIX) A ESCIN 0.74 A TRIPEPTIDE-1 0.001 A XANTHAN GUM 0.04 A CARRAGEENAN (CHONDRUS CRISPUS) 0.005 A CAPRYLYL GLYCOL 0.09 A PHENOXYETHANOL 0.09 A POTASSIUM SORBATE 0.03 B 1,3-PROPANEDIOL 8.50 C PHENOXYETHANOL 1.70 D AQUA (WATER) q.s.p. 100 E LECITHIN 10.00 F PEG-8 CAPRYLIC/CAPRIC GLYCERIDES 4.00

(20) The liposomes obtained were added to Luviquat HMM552 [INCI: Polyquaternium-16] in a cationic liposome:polymer ratio of 1.5:1 under light stirring.

Example 4

(21) Obtaining liposomes containing Serilesine® bound to cationic polymers of polyquaternium-16

(22) The liposomes were obtained in the same way as in example 1 but instead of Antarcticine®, Serilesine® (ingredients from phase A) was used and the same ingredients and quantities as in the other phases.

(23) TABLE-US-00004 % IN INGREDIENT (INCI Nomenclature) WEIGHT A GLYCERIN 1.00 A HEXAPEPTIDE-10 0.005 A CAPRYLYL GLYCOL 0.05 B 1,3-PROPANEDIOL 8.50 C PHENOXYETHANOL 1.70 D AQUA (WATER) q.s.p. 100 E LECITHIN 10.00 F PEG-8 CAPRYLIC/CAPRIC GLYCERIDES 4.00

(24) The liposomes obtained were added to Luviquat HMM552 [INCI: Polyquaternium-16] in a cationic liposome:polymer ratio of 1.5:1 under light stirring.

Example 5

(25) Obtaining liposomes containing hyaluronic acid and D-panthenol bound to cationic polymers of polyquaternium-16

(26) Water, Zemea [INCI: 1,3-propanediol] and phenoxyethanol (ingredients C to E) were added to a suitable vessel. When all the previous components had dissolved hyaluronic acid was added (ingredient A) slowly under light stirring. When it had dissolved, the D-panthenol (ingredient B) was added. Afterwards Leciflor 100IP [INCI: Lecithin] (ingredient F) was added slowly under intense stirring until fully dissolved. Next Labrasol [INCI: PEG-8 Caprylic/Capric Glycerides] (ingredient G) was added and was left stirring for 10-15 minutes so an emulsion was formed.

(27) TABLE-US-00005 % IN INGREDIENT (INCI Nomenclature) WEIGHT A HYALURONIC ACID 0.05 B DIETHYLAMINOHYDROXYBENZOYL HEXYL 8.50 BENZOATE C 1,3-PROPANEDIOL 8.50 D PHENOXYETHANOL 1.70 E AQUA (WATER) q.s.p. 100 F LECITHIN 10.00 G PEG-8 CAPRYLIC/CAPRIC GLYCERIDES 4.00

(28) The liposomes obtained were added to Luviquat HMM552 [INCI: Polyquaternium-16] in a cationic liposome: polymer ratio of 1.5:1 under light stirring.

Example 6

(29) Obtaining liposomes containing Lipochroman-6, Preventhelia™, Parsol MCX and Uvinul® A Plus bound to cationic polymers of polyquaternium-16

(30) Preventhelia™ (ingredients from phase A), part of the water, Zemea [INCI: 1,3-propanediol], Labrasol [INCI: PEG-8 Caprylic/Capric Glycerides] and phenoxyethanol (ingredients B to E) were added to a suitable vessel. When all the previous components had dissolved Leciflor 100IP [INCI: Lecithin] (ingredient F) was added slowly under intense stirring until fully dissolved. In another vessel Uvinul® A Plus [INCI: Diethylamino Hydroxybenzoyl Hexyl Benzoate], Parsol MCX [INCI: Ethylhexyl methoxycinnamate], Lipochroman-6 [INCI: Dimethylmethoxy Chromanol] (ingredients G to I) and the rest of the water were mixed together, heated to 60° C. to dissolve them. The second mixture was added to the first slowly under intense stirring.

(31) TABLE-US-00006 % IN INGREDIENT (INCI Nomenclature) WEIGHT A DIAMINOPROPIONOYL TRIPEPTIDE-33 0.0043 A CAPRYLYL GLYCOL 0.043 B 1,3-PROPANEDIOL 8.50 C PEG-8 CAPRYLIC/CAPRIC GLYCERIDES 4.00 D PHENOXYETHANOL 1.70 E AQUA (WATER) q.s.p. 100 F LECITHIN 10.00 G DIETHYLAMINO HYDROXYBENZOYL 5.00 HEXYL BENZOATE H ETHYLHEXYL METHOXYCINNAMATE 5.00 I DIMETHYLMETHOXY CHROMANOL 0.085

(32) The sample was passed through a microfluidizer for 3 cycles at an entrance pressure of 80 bar and 12500 psi on exit. The liposomes obtained were added to Luviquat HMM552 [INCI: Polyquaternium-16] in a cationic liposome: polymer ratio of 1.5:1 under light stirring.

Example 7

(33) Composition of a spray containing liposomes which contain Antarcticine bound to cationic polymers

(34) Antarcticine® (phase A), water, Zemea [INCI: 1,3-propanediol] and phenoxyethanol (ingredients B to D) were added to a suitable vessel. When all the previous components had dissolved it was heated to 40° C. and Emulmetik 320 [INCI: Hydrogenated Lecithin] (ingredient E) was added slowly under intense stirring until fully dissolved.

(35) TABLE-US-00007 % IN INGREDIENT (INCI Nomenclature) WEIGHT A PSEUDOALTEROMONAS FERMENT EXTRACT 0.78 A PHENOXYETHANOL, METHYLPARABEN, 0.01 BUTYLPARABEN, ETHYLPARABEN, PROPYLPARABEN, ISOBUTYLPARABEN B 1,3-PROPANEDIOL 8.50 C PHENOXYETHANOL 1.125 D AQUA (WATER) q.s.p. 100 E HYDROGENATED LECITHIN 0.85

(36) The sample was passed through a microfluidizer for 3 cycles at an entrance pressure of 80 bar and 12500 psi on exit. The liposomes obtained were added to Luviquat HMM552 [INCI: Polyquaternium-16] in a cationic liposome:polymer:water ratio of 8:1:1 under light stirring.

Example 8

(37) Composition of a spray containing liposomes which contain Antarcticine bound to cationic polymers

(38) Antarcticine® (phase A), water, Zemea [INCI: 1,3-propanediol] and phenoxyethanol (ingredients B to D) were added to a suitable vessel. When all the previous components had dissolved it was heated to 40° C. and Emulmetik 320 [INCI: Hydrogenated Lecithin] (ingredient E) was added slowly under intense stirring until fully dissolved.

(39) TABLE-US-00008 % IN INGREDIENT (INCI Nomenclature) WEIGHT A PSEUDOALTEROMONAS FERMENT EXTRACT 1.56 A PHENOXYETHANOL, METHYLPARABEN, 0.03 BUTYLPARABEN, ETHYLPARABEN, PROPYLPARABEN, ISOBUTYLPARABEN B 1,3-PROPANEDIOL 8.50 C PHENOXYETHANOL 1.125 D AQUA (WATER) q.s.p. 100 E HYDROGENATED LECITHIN 0.85

(40) The sample was passed through a microfluidizer for 3 cycles at an entrance pressure of 80 bar and 12500 psi on exit. The liposomes obtained were added to Luviquat HMM552 [INCI: Polyquaternium-16] in a cationic liposome: polymer ratio of 4:1 under light stirring.

Example 9

(41) Liposomes bound to cationic polymers containing Liporeductyl in textile materials, their washing and resistance to washing.

(42) The liposomes from example 3 were diluted in 50 mL water baths at 1%, 5%, 10% and 25% in weight. Different textile materials, such as polyamide (PA), cotton (CO 100%), polyester (PES), non-woven viscose (VISCOSA) and tights (TIGHTS), were submerged in each of the previous baths with ratios of textile material weight/bath of 1/25, 1/50 and 1/100 and they were kept submerged for 10 minutes at room temperature without stirring.

(43) To test the resistance of the washing of the liposomes adhered to the previous textile materials they were treated with detergent solutions under stirring according to standard ISO 105 CO6. The quantities of active ingredient bound to the textile material after the bath and after a number of washes were determined after the extraction of caffeine from the textile material through sonication for 10 minutes in a solution of pH 3.0. Afterwards it was heated and maintained at 60° C. for 30 minutes. The external phase was filtered (0.45 μm) and was analyzed by the HPLC-UV/VIS detector. The quantities of caffeine are shown in FIGS. 1 to 3.

(44) The textile materials were dried by being hung out according to standard UNE-EN ISO 6330 after the bath and after each wash. Typical drying times were 24 hours at room temperature.

Example 10

(45) Transmission electron microscopy of liposomes which contain Decelerine™ bound to cationic polymers.

(46) Images of liposomes from example 2 were taken using transmission electron microscopy (TEM) (FIG. 4). The presence of mono- and bilaminar spherical vesicles of 100 to 300 nm in diameter was observed. The cationic polymer chains of polyquaternium-16 could not be observed by this microscopy technique. The difference in the measurements according to this technique and dynamic light scattering is consistent with a structure wherein the liposome is embedded in a cationic network of cationic polymers.

Example 11

(47) Atomic Force Microscopy of liposomes which contain Decelerine™ bound to cationic polymers.

(48) Images of liposomes from example 2 were taken by atomic force microscopy. FIG. 5a shows an image of the mica substrate on its own. As the mica does not present any distortion on its structure, the color pattern is homogeneous. However, for the liposomes in example 2, FIG. 5b shows a layer with series of irregularities or small peaks which are the polyquaternium-16 layer or film on the mica substrate. Furthermore, vesicular structures in a clearer color than the liposomes to which the polyquaternium-16 polymeric chains are bound were observed.

Example 12

(49) Test of transfer to skin of the active ingredients contained in liposomes of Liporeductyl® in textile materials.

(50) The liposomes obtained in example 3 were taken and 100 μL of 2% weight aqueous solution of these liposomes was applied directly with a micropipette to a piece of cotton fabric with a surface area of 1,77 cm2.

(51) Next the transfer test was carried out with pig skin, in accordance with a modification of the OCDE standard 428 (Skin absorption: in vitro method). The modification consisted of applying a weight to the fabric sample placed on the pig skin to simulate the pressure that the skin would exert on the fabric. The weight applied was 1 g. After 24 hours of exposure of the cotton fabric to the skin the quantity of caffeine to have reached the surface of the skin as well as the different layers of skin was determined by HPLC. It was obtained that with a pressure even as small as 0.56 g/cm2, the percentage of caffeine which passed through the surface and layer of skin was 0.40%.

(52) While exemplary embodiments are described above, it is not intended that these embodiments describe all possible forms of the invention. Rather, the words used in the specification are words of description rather than limitation, and it is understood that various changes may be made without departing from the spirit and scope of the invention. Additionally, the features of various implementing embodiments may be combined to form further embodiments of the invention.