Plasminogen activator inhibitor-1 inhibitors and methods of use thereof

Abstract

The invention relates to plasminogen activator-1 (PAI-1) inhibitor compounds and uses thereof in the treatment of any disease or disorder associated with elevated PAI-1. The invention includes, but is not limited to, the use of such compounds to prevent or reduce thrombosis and fibrosis, to promote thrombolysis, and to modulate lipid metabolism and treat diseases or disorders associated with elevated PAI-1, cholesterol, or lipid levels.

Claims

1. A compound of formula I or a salt thereof: ##STR00267## wherein: R.sup.1 is selected from the group consisting of dihalophenyl, trihalophenyl, fluoro(trifluoromethyl)phenyl, chloro(trifluoromethyl)phenyl, bromo(trifluoromethyl)phenyl, iodo(trifluoromethyl)phenyl, xylyl, fluorotolyl, chlorotolyl, bromotolyl, iodotolyl, fluoroxylyl, chloroxylyl, bromoxylyl, iodoxylyl, (trifluoromethoxy)phenyl, and cyanophenyl, each of which may be optionally substituted; R.sup.2 is -L.sup.5-C(═O)R.sup.3; R.sup.3 is NHNHR.sup.9; R.sup.9 and R.sup.17 are independently selected from the group consisting of H and C.sub.1 to C.sub.12 alkyl; and L.sup.5 is selected from the group consisting of null, C.sub.1 to C.sub.12 alkylene, and C.sub.1 to C.sub.12 alkenylene.

2. The compound or salt of claim 1 having formula VII: ##STR00268##

3. The compound or salt of claim 1, wherein R.sup.1 is selected from the group consisting of: ##STR00269##

4. The compound or salt of claim 1 having a formula selected from the group consisting of C251, C255, C261, C262, C268, C278, C282, C287, C301, C304, C305, C307, C336, C340, and C341: ##STR00270## ##STR00271##

5. A composition comprising the compound or salt according to claim 1 and a pharmaceutically acceptable carrier.

6. A method of treating a disease or disorder associated with an elevated level of PAI-1 in a subject comprising administering an effective amount of a composition comprising the compound or salt according to claim 1 and a pharmaceutically acceptable carrier to decrease the elevated level of PAI-1 in the subject, wherein the disease or disorder is obesity, insulin resistance, a disease or disorder associated with dysregulation of lipid metabolism, a disease or disorder associated with an elevated level of VLDL or LDL, high cholesterol, fibrosis and fibrotic disease, cerebrovascular disease, fibrinolytic disorder, stroke, coronary heart disease, myocardial infarction, thrombosis, deep vein thrombosis, diabetes, or fibrinolytic impairment.

7. A method of modulating cholesterol, lipid clearance, and/or lipid uptake in a subject with an elevated level of PAI-1 comprising administering an effective amount of a composition comprising the compound or salt according to claim 1 and a pharmaceutically acceptable carrier in an amount effective to decrease the elevated level of PAI and modulate cholesterol, lipid clearance, and/or lipid uptake in the subject.

8. The method of claim 7, wherein the composition increases circulating high density lipoprotein (HDL) and/or decreases circulating very low density lipoprotein (VLDL) in the subject.

9. The method of claim 7, wherein the composition inhibits apolipoprotein E (ApoE) or apolipoprotein A (ApoA) binding to VLDL-R.

10. The method of claim 7, wherein the composition affects HDL or apolipoprotein E (ApoE) or apolipoprotein A (ApoA) binding to an ApoA receptor.

11. The method of claim 7, wherein the composition decreases PAI-1 binding to apolipoprotein E (ApoE), apolipoprotein A (ApoA), and/or VLDL.

12. The method of claim 7, wherein the composition binds to PAI-1 in the presence of vitronectin and/or urokinase type plasminogen activator (uPA).

Description

DETAILED DESCRIPTION OF THE INVENTION

(1) The invention describes materials and methods for the inhibition of plasminogen activator inhibitor-1 (PAI-1). In exemplary aspects, the invention describes PAI-1 inhibitor compounds.

(2) PAI-1 Inhibitor Compounds of the Invention

(3) As used herein, the term “haloalkyl” refers to a hydrocarbon group substituted with one or more halogens selected from F, Cl, Br, and I.

(4) As used herein, the term “cycloalkyl” refers to a cyclic hydrocarbon group, e.g., cyclopropyl, cyclobutyl, cyclohexyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl. As used herein, the term “heterocycloalkyl” or “heterocyclic ring” refers to a cyclic hydrocarbon group having one or more heteroatoms, for example, one to three heteroatoms, independently selected from the group consisting of oxygen, nitrogen, and sulfur.

(5) As used herein, the term “aryl” refers to a monocyclic or polycyclic aromatic group, preferably a monocyclic or bicyclic aromatic group. Unless otherwise indicated, an aryl group can be unsubstituted or substituted with one or more, and in particular one to four, groups independently selected from, for example, —OH, —OR (including —OCH.sub.3), —F, —Cl, —Br, —I, —CF.sub.3, —NO.sub.2, —NO, —N(R).sub.2, —N(R).sub.3.sup.+, —C(O)R, —C(O)OR, —CHO, —C(O)NH.sub.2, —C(O)SR, —CN, —S(O).sub.2R, —SO.sub.3R, —SO.sub.3H, —SO.sub.2N(R).sub.2, —S═O, aryl, and heteroaryl. Exemplary aryl groups include, but are not limited to, phenyl, tolyl, naphthyl, tetrahydronaphthyl, chlorophenyl, methylphenyl, methoxyphenyl, trifluoromethylphenyl, nitrophenyl, 2,4-methoxychlorophenyl, and the like.

(6) As used herein, the term “heteroaryl” refers to a monocyclic or polycyclic ring system containing one or more aromatic rings and containing at least one nitrogen, oxygen, or sulfur atom in an aromatic ring. Unless otherwise indicated, a heteroaryl group can be unsubstituted or substituted with one or more, and in particular one to four, substituents selected from, for example, —OH, —OR (including —OCH.sub.3), —F, —Cl, —Br, —I, —CF.sub.3, —NO.sub.2, —NO, —N(R).sub.2, —N(R).sub.3.sup.+, —C(O)R, —C(O)OR, —CHO, —C(O)NH.sub.2, —C(O)SR, —CN, —S(O).sub.2R, —SO.sub.3R, —SO.sub.3H, —SO.sub.2N(R).sub.2, —S═O, aryl, and heteroaryl. Examples of heteroaryl groups include, but are not limited to, thienyl, furyl, pyridyl, oxazolyl, quinolyl, thiophenyl, isoquinolyl, indolyl, triazinyl, triazolyl, isothiazolyl, isoxazolyl, imidazolyl, benzothiazolyl, pyrazinyl, pyrimidinyl, thiazolyl, and thiadiazolyl.

(7) As used herein, the term “substituted benzyl” refers to a benzyl group substituted with one or more, and in particular one to four, groups independently selected from, for example, —OH, —OR (including —OCH.sub.3), —F, —Cl, —Br, —I, —CF.sub.3, —NO.sub.2, —NO, —N(R).sub.2, —N(R).sub.3.sup.+, —C(O)R, —C(O)OR, —CHO, —C(O)NH.sub.2, —C(O)SR, —CN, —S(O).sub.2R, —SO.sub.3R, —SO.sub.3H, —SO.sub.2N(R).sub.2, —S═O, aryl, and heteroaryl.

(8) As used herein, the term “amino acid” refers to naturally occurring and non-natural amino acids, as well as amino acid analogs. Naturally encoded amino acids include the 20 common amino acids (alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine), pyrrolysine, and selenocysteine. Amino acid analogs refers to compounds that have the same basic chemical structure as a naturally occurring amino acid, i.e., a carbon that is bound to a hydrogen, a carboxyl group, an amino group, and an R group, for example, 3-nitrotyrosine, homoserine, norleucine, methionine sulfoxide, methionine methyl sulfonium. Such analogs have modified R groups (such as 3-nitrotyrosine) or modified peptide backbones, but retain the same basic chemical structure as a naturally occurring amino acid. Amino acid analogs also include amino acid esters (e.g., amino acid alkyl esters, such as amino acid methyl esters) and acylated amino acids (e.g., acetylated amino acids).

(9) It will also be appreciated that certain of the compounds of present invention can exist in free form for treatment, or where appropriate, as a pharmaceutically acceptable derivative thereof. According to the present invention, a pharmaceutically acceptable derivative includes, but is not limited to, pharmaceutically acceptable salts, esters, salts of such esters, prodrugs, salts of such prodrugs, or any other adduct or derivative which upon administration to a patient in need is capable of providing, directly or indirectly, a compound as otherwise described herein.

(10) As used herein, the term “pharmaceutically acceptable salt” refers to those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio. A “pharmaceutically acceptable salt” means any salt or salt of an ester of a compound of this invention that, upon administration to a recipient, is capable of providing, either directly or indirectly, a compound of this invention.

(11) Pharmaceutically acceptable salts are well known in the art. For example, S. M. Berge et al. describe pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 1977, 66, 1-19, which is incorporated herein by reference. Pharmaceutically acceptable salts of the compounds of this invention include those derived from suitable inorganic and organic acids and bases. Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, trifluoroacetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange. Other pharmaceutically acceptable salts include adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, glutamate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate, tartrate, thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and the like. Salts of compounds containing a carboxylic acid or other acidic functional group can be prepared by reacting with a suitable base. Such salts include, but are not limited to, alkali metal, alkaline earth metal, aluminum salts, ammonium, N.sup.+(C.sub.1-4alkyl).sub.4 salts, and salts of organic bases such as trimethylamine, triethylamine, morpholine, pyridine, piperidine, picoline, dicyclohexylamine, N,N′-dibenzylethylenediamine, 2-hydroxyethylamine, bis-(2-hydroxyethyl)amine, tri-(2-hydroxyethyl)amine, procaine, dibenzylpiperidine, dehydroabietylamine, N,N′-bisdehydroabietylamine, glucamine, N-methylglucamine, collidine, quinine, quinoline, and basic amino acids such as lysine and arginine. This invention also envisions the quaternization of any basic nitrogen-containing groups of the compounds disclosed herein. Water or oil-soluble or dispersible products may be obtained by such quaternization. Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like. Further pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, lower alkyl sulfonate and aryl sulfonate.

(12) The term “prodrug” as used herein refers to compounds that are rapidly converted to an active form (i.e., drug) within the body or cells thereof by the action of endogenous enzymes or other chemicals and/or conditions. Prodrug design is discussed generally in Hardma et al. (Eds.), Goodman and Gilman's The Pharmacological Basis of Therapeutics, 9th ed., pp. 11-16 (1996). Prodrugs of the compounds disclosed herein include, but are not limited to, esters formed from available hydroxyl or carboxyl groups (also referred to as ester prodrugs or prodrug esters), amides formed from available amino, amido, or carboxyl groups, thioesters formed from available thiol or carboxyl groups, carbonates formed from available hydroxyl or carboxyl groups, carbamates formed from available hydroxyl, amino, or amido groups, carbamides formed from available amido or amino groups, sulfonate esters and sulfate esters formed from available hydroxyl groups, sulfonamides formed from available amino groups, and phosphonamides formed from available amino groups. Suitable ester prodrugs include, but are not limited to, aliphatic esters, aryl esters, benzyl esters, and derivatives thereof.

(13) Compounds of the invention include those of formula I or a salt, ester, or prodrug thereof:

(14) ##STR00001##

(15) wherein:

(16) R.sup.1 is selected from the group consisting of C.sub.1 to C.sub.12 alkyl, -L.sup.1-C.sub.3-C.sub.6 cycloalkyl, -L.sup.2-C.sub.2-C.sub.6 heterocycloalkyl, benzyl, -L.sup.3-aryl, and -L.sup.4-heteroaryl;

(17) R.sup.2 is selected from the group consisting of -L.sup.5-C(═O)R.sup.3, -L.sup.6-R.sup.4, and NHR.sup.5;

(18) R.sup.3 is selected from the group consisting of OR.sup.6, NR.sup.7R.sup.8, and NHNHR.sup.9;

(19) R.sup.5 is selected from the group consisting of OR.sup.10, C.sub.1 to C.sub.12 alkyl, -L.sup.7-C.sub.3-C.sub.6 cycloalkyl, -L.sup.8-C.sub.2-C.sub.6 heterocycloalkyl, benzyl, -L.sup.9-aryl, and -L.sup.10-heteroaryl;

(20) R.sup.8 is selected from the group consisting of OR.sup.11, N═R.sup.12R.sup.13, -L.sup.11-R.sup.14, NHSO.sub.2R.sup.15, and NHR.sup.16;

(21) R.sup.6, R.sup.7, R.sup.9, R.sup.10, R.sup.11, R.sup.12, R.sup.13, and R.sup.17 are independently selected from the group consisting of H and C.sub.1 to C.sub.12 alkyl;

(22) R.sup.4, R.sup.14, R.sup.15, and R.sup.16 are independently selected from the group consisting of -L.sup.12-C.sub.3-C.sub.6 cycloalkyl, -L.sup.13-C.sub.2-C.sub.6 heterocycloalkyl, benzyl, -L.sup.14-aryl, and -L.sup.15-heteroaryl; and

(23) L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5, L.sup.6, L.sup.7, L.sup.8, L.sup.9, L.sup.10, L.sup.11, L.sup.12, L.sup.13, L.sup.14 and L.sup.15 are independently selected from the group consisting of null, C.sub.1 to C.sub.12 alkylene, and C.sub.1 to C.sub.12 alkenylene.

(24) Compounds of the invention include those of formula II or a salt, ester, or prodrug thereof:

(25) ##STR00002##
wherein R.sup.1, L.sup.5, and R.sup.3 are as defined above for formula I.

(26) Compounds of the invention include those of formula III or a salt, ester, or prodrug thereof:

(27) ##STR00003##
wherein R.sup.1, L.sup.6, and R.sup.4 are as defined above for formula I.

(28) Compounds of the invention include those of formula IV or a salt, ester, or prodrug thereof:

(29) ##STR00004##
wherein R.sup.1 and R.sup.5 are as defined above for formula I.

(30) Compounds of the invention include those of formula V or a salt, ester, or prodrug thereof:

(31) ##STR00005##
wherein R.sup.1, L.sup.5, and R.sup.6 are as defined above for formula I.

(32) Compounds of the invention include those of formula VI or a salt, ester, or prodrug thereof:

(33) ##STR00006##
wherein R.sup.1, L.sup.5, R.sup.7, and R.sup.8 are as defined above for formula I.

(34) Compounds of the invention include those of formula VII or a salt, ester, or prodrug thereof:

(35) ##STR00007##
wherein R.sup.1, L.sup.5, and R.sup.9 are as defined above for formula I.

(36) In formulas I to VII, R.sup.1 is selected from C.sub.1 to C.sub.12 alkyl, -L.sup.1-C.sub.3-C.sub.6 cycloalkyl, -L.sup.2-C.sub.2-C.sub.6 heterocycloalkyl, benzyl, -L.sup.3-aryl, and -L.sup.4-heteroaryl, and is optionally substituted with one, two, three, or more substituent groups that are the same or different. Suitable substituent groups include, but are not limited to, F, Cl, Br, I, CF.sub.3, CH.sub.3, OCF.sub.3, OCH.sub.3, and —CN. Additional R.sup.1 groups include, but are not limited to, optionally substituted phenyl, halophenyl (e.g., fluorophenyl, chlorophenyl, bromophenyl, iodophenyl), dihalophenyl (e.g., difluorophenyl, dichlorophenyl, dibromophenyl, diiodophenyl), trihalophenyl (e.g., trifluorophenyl, trichlorophenyl, tribromophenyl, triiodophenyl), (trifluoromethyl)phenyl, fluoro(trifluoromethyl)phenyl, chloro(trifluoromethyl)phenyl, bromo(trifluoromethyl)phenyl, iodo(trifluoromethyl)phenyl, tolyl, xylyl, fluorotolyl, chlorotolyl, bromotolyl, iodotolyl, fluoroxylyl, chloroxylyl, bromoxylyl, iodoxylyl, methoxyphenyl, dimethoxyphenyl, (trifluoromethoxy)phenyl, cyanophenyl, dimethoxybenzyl, methylisoxazolyl, 3H-1,3,4-oxadiazol-2-one-5-yl, methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl, undecyl, and dodecyl. Further R.sup.1 groups include

(37) ##STR00008## ##STR00009##
pentyl, and butyl.

(38) R.sup.4, R.sup.14, R.sup.15, and R.sup.16 are independently selected from -L.sup.12-C.sub.3-C.sub.6 cycloalkyl, -L.sup.13-C.sub.2-C.sub.6 heterocycloalkyl, benzyl, -L.sup.14-aryl, and -L.sup.15-heteroaryl, and are optionally substituted with one, two, three, or more substituent groups that are the same or different. Suitable substituent groups include, but are not limited to, F, Cl, Br, I, CF.sub.3, CH.sub.3, OCF.sub.3, OCH.sub.3, and —CN. Additional R.sup.4, R.sup.14, R.sup.15, and R.sup.16 groups include, but are not limited to, optionally substituted phenyl, halophenyl (e.g., fluorophenyl, chlorophenyl, bromophenyl, iodophenyl), dihalophenyl (e.g., difluorophenyl, dichlorophenyl, dibromophenyl, diiodophenyl), trihalophenyl (e.g., trifluorophenyl, trichlorophenyl, tribromophenyl, triiodophenyl), (trifluoromethyl)phenyl, fluoro(trifluoromethyl)phenyl, chloro(trifluoromethyl)phenyl, bromo(trifluoromethyl)phenyl, iodo(trifluoromethyl)phenyl, tolyl, xylyl, fluorotolyl, chlorotolyl, bromotolyl, iodotolyl, fluoroxylyl, chloroxylyl, bromoxylyl, iodoxylyl, methoxyphenyl, dimethoxyphenyl, (trifluoromethoxy)phenyl, cyanophenyl, dimethoxybenzyl, methylisoxazolyl, and 3H-1,3,4-oxadiazol-2-one-5-yl. Further R.sup.4 groups include

(39) ##STR00010##

(40) R.sup.5 is selected from OR.sup.10, C.sub.1 to C.sub.12 alkyl, -L.sup.7-C.sub.3-C.sub.6 cycloalkyl, -L.sup.8-C.sub.2-C.sub.6 heterocycloalkyl, benzyl, -L.sup.9-aryl, and -L.sup.10-heteroaryl, and is optionally substituted with one, two, three, or more substituent groups that are the same or different. Suitable substituent groups include, but are not limited to, F, Cl, Br, I, CF.sub.3, CH.sub.3, OCF.sub.3, OCH.sub.3, and —CN. Additional R.sup.5 groups include, but are not limited to, optionally substituted phenyl, halophenyl (e.g., fluorophenyl, chlorophenyl, bromophenyl, iodophenyl), dihalophenyl (e.g., difluorophenyl, dichlorophenyl, dibromophenyl, diiodophenyl), trihalophenyl (e.g., trifluorophenyl, trichlorophenyl, tribromophenyl, triiodophenyl), (trifluoromethyl)phenyl, fluoro(trifluoromethyl)phenyl, chloro(trifluoromethyl)phenyl, bromo(trifluoromethyl)phenyl, iodo(trifluoromethyl)phenyl, tolyl, xylyl, fluorotolyl, chlorotolyl, bromotolyl, iodotolyl, fluoroxylyl, chloroxylyl, bromoxylyl, iodoxylyl, methoxyphenyl, dimethoxyphenyl, (trifluoromethoxy)phenyl, cyanophenyl, dimethoxybenzyl, methylisoxazolyl, 3H-1,3,4-oxadiazol-2-one-5-yl, methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl, undecyl, and dodecyl. Further R.sup.5 groups include OH.

(41) R.sup.6, R.sup.7, R.sup.9, R.sup.10, R.sup.11, R.sup.12, R.sup.13, and R.sup.17 are independently selected from H and C.sub.1 to C.sub.12 alkyl, including C.sub.1 alkyl, C.sub.2 alkyl, C.sub.3 alkyl, C.sub.4 alkyl, C.sub.5 alkyl, C.sub.6 alkyl, C.sub.7 alkyl, C.sub.8 alkyl, C.sub.9 alkyl, C.sub.10 alkyl, C.sub.11 alkyl, and C.sub.12 alkyl.

(42) R.sup.8 is selected from OR.sup.11, N═R.sup.12R.sup.13, -L.sup.11-R.sup.14, NHSO.sub.2R.sup.15, and NHR.sup.16. Exemplary R.sup.8 groups include, but are not limited to, OH, OCH.sub.3, N═(CH.sub.3).sub.2,

(43) ##STR00011##

(44) L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5, L.sup.6, L.sup.7, L.sup.8, L.sup.9, L.sup.10, L.sup.11, L.sup.12, L.sup.13, L.sup.14, and L.sup.15 are independently selected from null (a bond), C.sub.1 to C.sub.12 alkylene, including C.sub.1 alkylene, C.sub.2 alkylene, C.sub.3 alkylene, C.sub.4 alkylene, C.sub.5 alkylene, C.sub.6 alkylene, C.sub.7 alkylene, C.sub.8 alkylene, C.sub.9 alkylene, C.sub.10 alkylene, C.sub.11 alkylene, and C.sub.12 alkylene, and C.sub.1 to C.sub.12 alkenylene, including C.sub.1 alkenylene, C.sub.2 alkenylene, C.sub.3 alkenylene, C.sub.4 alkenylene, C.sub.5 alkenylene, C.sub.6 alkenylene, C.sub.7 alkenylene, C.sub.8 alkenylene, C.sub.9 alkenylene, C.sub.10 alkenylene, C.sub.11 alkenylene, and C.sub.12 alkenylene.

(45) Exemplary compounds of the invention have a formula selected from C256, C259, C265, C267, C276, C277, C288, C309, C311, C280, C300, C313, C314, C320, C323, C326, C328, C334, C342, C240, C241, C246, C248, C251, C255, C260, C261, C262, C263, C264, C266, C268, C278, C281, C282, C287, C289, C295, C296, C297, C301, C304, C305, C307, C310, C322, C336, C339, C340, C341, C362, C279, C285, C286, C299, C306, C330, C344, C345, C346, C347, C348, C356, C357, C358, C359, C360, C361, C363, C364, C284, and salts, esters, or prodrugs thereof. These compounds are depicted in Table 1 herein below.

(46) TABLE-US-00001 TABLE 1 Synthesized PAI-1 Inhibitor Compounds PAI-1/ uPA PAI-1/ IC.sub.50 in uPA IC.sub.50 PAI-1/ 1.5% in 10% uPA IC.sub.50 BSA plasma in buffer buffer buffer No. Structure (μM) (μM) (μM) C256 1040, 1353 204, 235  214 C259 2892, 3141 625, 760  526 C265 4018 C267 3794 C276 1438  428  476 C277 750.6, 719 165.4, 158 148, 142 C288 2909 1061  752 C309 1200 C311 0 C280 207, 203 166, 159  168 C300 5491 C313 1196  532  490 C314 C320  329  513  693 C323 C326 C328  713  345  318 C334  189 C342 0 C240 3766, 3912 2221, 1973 1208 C241 1933 C246 2559 C248 1190, 1313 652, 646  478 C251 68.6, 76 110, 123  108 C255 2064, 1780 221, 292  279 C260 147, 187 43, 545  525 C261 102, 103 68, 86  69 C262 170, 187 63, 84  86 C263 0 2837 1685 1356 C264 2121 1175 1248 C266 568, 549  126  219 C268 135, 134  65  77 C278 640, 642 118, 130  142 C281 2285 C282 225, 219 163, 154  121 C287 1187  363  304 C289 2725 1099  733 C295 1337  776  736 C296 0  112  69  64 C297  136  111  87 C301  173  128  106 C304  162  90  74 C305  224  81  62 C307  465  99  101 C310 C322 4315 C336 5469  892  851 C339  282  247  303 C340 0  337  249  272 C341  900 1460 1868 C362  533  353  229 C279 539, 593 545, 504  680 C285 422, 388  265  306 C286  580 C299  238  739  628 C306 1159  403  405 C330 365, 423*  112  115 C344 1002  830  866 C345 0 1469  673  539 C346  353 C347  888  309  293 C348  99  454  240 C356 1100 1241  544 C357 1370 1227  567 C358 1501 1229  619 C359 3373 2084  974 C360  164 1670  771 C361  232 2000  851 C363 0  199  751  309 C364  475  898  533 C284 2481 *pH 7.8 was used.

(47) Comparative compounds that do not demonstrate PAI-1 inhibitory activity in buffer (40 mM HEPES, 100 mM NaCl, 0.05% Tween-20, 10% DMSO, pH 7.4) in the assay described in Example 11 herein are depicted in Table 2.

(48) TABLE-US-00002 TABLE 2 Synthesized Comparative Compounds No. Structure E242 E243 E244 E245 E247 E254 E257 E258 0 E290 E319 E308 E312 E324 E325 E329 E331 E332 E335 00 E343 01 E338 02 E234 03 E235 04 E237 05 E238 06 E252 07 E253 08 E269 09 E291 0 E327 E333 E321 E349 E350 E351 E352 E353 E354 E355 0 E283 E298

(49) Compounds of the invention include those of formula VIII or a salt, ester, or prodrug thereof:

(50) ##STR00123##

(51) wherein:

(52) W is C or N;

(53) X.sub.1, X.sub.2, X.sub.3, X.sub.4, and X.sub.5 are independently selected from the group consisting of —H, —OH, —OR, —F, —Cl, —Br, —I, —NO.sub.2, —NO, —N(R).sub.2, —N(R).sub.3.sup.+, —C(O)R, —C(O)OR, —CHO, —C(O)NH.sub.2, —C(O)SR, —CN, —S(O).sub.2R, —SO.sub.3R, —SO.sub.3H, —SO.sub.2N(R).sub.2, —S═O, C.sub.1 to C.sub.12 alkyl, aryl, substituted aryl, heteroaryl, and substituted heteroaryl;

(54) R is selected from the group consisting of C.sub.1 to C.sub.6 alkyl, C.sub.3 to C.sub.6 cycloalkyl, CH.sub.2—C.sub.3-C.sub.6 cycloalkyl, phenyl, tolyl, and benzyl;

(55) R.sup.a is selected from the group consisting of C.sub.1 to C.sub.12 alkyl, C.sub.3 to C.sub.6 cycloalkyl, (CH.sub.2).sub.m—C.sub.3-C.sub.6 cycloalkyl, C.sub.2 to C.sub.6 heterocycloalkyl, (CH.sub.2).sub.m—C.sub.2-C.sub.6 heterocycloalkyl, C.sub.2 to C.sub.6 heterocycloalkyl, (CH.sub.2).sub.m—C.sub.2-C.sub.6 heterocycloalkyl, benzyl, aryl, (CH.sub.2).sub.m-aryl, heteroaryl, (CH.sub.2).sub.m-heteroaryl, and substituted derivatives thereof; and

(56) m is 1, 2, 3, 4, 5, or 6.

(57) In some embodiments, compounds of the invention include those of formula VIII as defined above with the proviso that at most two of X.sub.1, X.sub.2, X.sub.3, X.sub.4, and X.sub.5 are OH.

(58) In some embodiments, compounds of the invention include those of formula VIII as defined above excluding compounds having a formula

(59) ##STR00124##

(60) In some embodiments, compounds of the invention include those of formula VIII as defined above with the proviso that at most three of X.sub.1, X.sub.2, X.sub.3, X.sub.4, and X.sub.5 are H.

(61) Compounds of the invention include those of formula IX or a salt, ester, or prodrug thereof:

(62) ##STR00125##

(63) wherein R.sup.a is selected from the group consisting of benzyl, aryl, (CH.sub.2).sub.m-aryl, heteroaryl, (CH.sub.2).sub.m-heteroaryl, and substituted derivatives thereof; and

(64) m is 1, 2, 3, 4, 5, or 6.

(65) In some embodiments, compounds of the invention include those of formula VIII or IX as defined above wherein R.sup.a is selected from the group consisting of:

(66) ##STR00126##

(67) wherein: W is C or N; and

(68) R.sup.1 and R.sup.2 are independently selected from the group consisting of —H, —F, —Cl, —Br, —I, —CF.sub.3, C.sub.1 to C.sub.12 alkyl, and phenyl.

(69) Exemplary compounds of the invention have a formula selected from C152, C155, C173, C189, C191, C197, C224, C292, C293, C294, C153, C162, C163, C165, C188, C195, C157, C158, C182, C183, and salts, esters, or prodrugs thereof. These compounds are depicted in Table 3 herein below.

(70) TABLE-US-00003 TABLE 3 Synthesized PAI-1 Inhibitor Compounds PAI-1/ PAI-1/ ATIII/ uPA tPA αIIa IC.sub.50 IC.sub.50 IC.sub.50 No. Structure (μM) (μM) (μM) C152  1.57  1.71 87   C155 30.44 34.84 2326    C173 19.2  C189 0  2.29 C191  1.28 C197  0.173 C224  4.34 C292 4.94, 68* C293 77.6, 123* C294 120.5, 234* C153  0.70  1.02 C162  3.90  7.59 C163  0.288  0.611 C165 0  0.35  0.51 C188  0.12 C195  0.92 C157  0.25  0.98 44.37 C158  2.60  1.44 547    C182  0.033 C183  0.18 *pH 7.4 was used.

(71) Comparative compounds that do not demonstrate PAI-1 inhibitory activity in buffer (40 mM HEPES, 100 mM NaCl, 0.05% Tween-20, 10% DMSO, pH 7.8) in the assay described in Example 12 herein are depicted in Table 4.

(72) TABLE-US-00004 TABLE 4 Synthesized Comparative Compounds No. Structure E174

(73) Compounds of the invention include those of formula X or a salt, ester, or prodrug thereof:

(74) ##STR00148##

(75) wherein

(76) R.sup.a is C.sub.1 to C.sub.12 alkyl,

(77) R.sup.b is selected from the group consisting of C.sub.1 to C.sub.12 alkyl, aryl, heteroaryl, (CH.sub.2).sub.m—R, and

(78) ##STR00149##
or

(79) R.sup.a and R.sup.b taken together with the N atom to which they are bonded form an optionally substituted 3- to 8-membered heterocyclic ring;

(80) m is 1, 2, 3, 4, 5, or 6;

(81) n is 0, 1, 2, 3, 4, 5, or 6;

(82) Y is selected from the group consisting of NH.sub.2 and OH; and

(83) R selected from the group consisting of

(84) ##STR00150##
substituted phenyl and heteroaryl.

(85) In some embodiments, compounds of the invention include those of formula X as defined above wherein R.sup.a is selected from the group consisting of butyl, pentyl, hexyl, heptyl, octyl, nonyl, and decyl. In some embodiments, compounds of the invention include those of formula X as defined above wherein R.sup.b is selected from the group consisting of butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl, fluorophenyl, chlorophenyl, bromophenyl, iodophenyl, trifluoromethylphenyl, and dichlorohydroxyphenyl.

(86) Compounds of the invention include those of formula XI or a salt, ester, or prodrug thereof:

(87) ##STR00151##

(88) wherein

(89) R.sup.b is selected from the group consisting of aryl, heteroaryl, (CH.sub.2).sub.m—R, and

(90) ##STR00152##

(91) m is 1, 2, 3, 4, 5, or 6;

(92) n is 0, 1, 2, 3, 4, 5, or 6;

(93) Y is selected from the group consisting of NH.sub.2 and OH; and

(94) R selected from the group consisting of

(95) ##STR00153##
CO.sub.2H, phenyl, substituted phenyl and heteroaryl.

(96) Compounds of the invention include those of formula XII or a salt, ester, or prodrug thereof:

(97) ##STR00154##

(98) wherein

(99) R.sup.b is selected from the group consisting of aryl, heteroaryl, (CH.sub.2).sub.m—R, and

(100) ##STR00155##

(101) m is 1, 2, 3, 4, 5, or 6;

(102) n is 0, 1, 2, 3, 4, 5, or 6;

(103) Y is selected from the group consisting of NH.sub.2 and OH; and

(104) R selected from the group consisting of

(105) ##STR00156##
CO.sub.2H, phenyl, substituted phenyl and heteroaryl.

(106) Exemplary compounds of the invention have a formula selected from C170, C171, C172, C175, C177, C179, C180, C186, C193, C205, C160, C187, C190, C198, C232, C233, C249, C270, C271, C272, C273, C274, C275, C303, C210, and salts, esters, or prodrugs thereof. These compounds are depicted in Table 5 herein below.

(107) TABLE-US-00005 TABLE 5 Synthesized PAI-1 Inhibitor Compounds PAI-1/ PAI-1/ ATIII/ uPA tPA αIIa IC.sub.50 IC.sub.50 IC.sub.50 No. Structure (μM) (μM) (μM) C170  5.49 C171  1.67 C172  1.98 C175 0  6.74 C177  5.11 C179  2.40 C180  1.34 C186  6.98 C193 552    C205  5.05 C160 39.04 58.16 2326 C187  0.91 C190  9.70 C198 0  0.051 C232 13.3  C233 74.8  C249 663    C270 29.5  C271  1.82 C272 16.4  C273 55.6  C274 73.6  C275 30.5  C303 0 12.6*, 13.6 C210 189.6  *pH 7.4 was used.

(108) Comparative compounds that do not demonstrate PAI-1 inhibitory activity in buffer (40 mM HEPES, 100 mM NaCl, 0.05% Tween-20, 10% DMSO, pH 7.8) in the assay described in Example 12 herein are depicted in Table 6.

(109) TABLE-US-00006 TABLE 6 Synthesized Comparative Compounds No. Structure E169 E178 E192 E194

(110) A comparative compound that does not demonstrate PAI-1 inhibitory activity in buffer (40 mM HEPES, 100 mM NaCl, 0.05% Tween-20, 10% DMSO, pH 7.4) in the assay described in Example 11 herein is depicted below.

(111) ##STR00186##

(112) Exemplary compounds of the invention have a formula selected from C168, C176, C184, C185, C196, C156, C161, C200, C204, C236, and salts, esters, or prodrugs thereof. These compounds are depicted in Table 7 herein below.

(113) TABLE-US-00007 TABLE 7 Synthesized PAI-1 Inhibitor Compounds PAI-1/ ATIII/ PAI-1/ tPA αIIa uPA IC.sub.50 IC.sub.50 IC.sub.50 No. Structure (μM) (μM) (μM) C168 1.98 C176 0.62 C184 0.59 C185 0 0.42 C196 0.45 C156 188.7 581.2 C161 3.70 2.21 1670 C200 58.37 C204 0.035 C236 174

(114) Comparative compounds that do not demonstrate PAI-1 inhibitory activity in buffer (40 mM HEPES, 100 mM NaCl, 0.05% Tween-20, 10% DMSO, pH 7.4) in the assay described in Example 11 herein are depicted in Table 8.

(115) TABLE-US-00008 TABLE 8 Synthesized Comparative Compounds No. Structure E164 E250 E181 E167 00 E166 01

(116) Compounds of the invention include those of formula XIII or a salt, ester, or prodrug thereof:

(117) ##STR00202##

(118) wherein

(119) n is 0 or 1;

(120) R.sup.a and R.sup.b are independently selected from the group consisting of C.sub.1 to C.sub.12 alkyl, C.sub.3 to C.sub.6 cycloalkyl, (CH.sub.2).sub.m—C.sub.3-C.sub.6 cycloalkyl, C.sub.2 to C.sub.6 heterocycloalkyl, (CH.sub.2).sub.m—C.sub.2-C.sub.6 heterocycloalkyl, C.sub.2 to C.sub.6 heterocycloalkyl, (CH.sub.2).sub.m—C.sub.2-C.sub.6 heterocycloalkyl, benzyl, aryl, (CH.sub.2).sub.m-aryl, heteroaryl, (CH.sub.2).sub.m-heteroaryl, and substituted derivatives thereof; and

(121) m is 1, 2, 3, 4, 5, or 6.

(122) In some embodiments, compounds of the invention include those of formula XIII as defined above wherein R.sup.a and R.sup.b are independently selected from the group consisting of butyl, pentyl, cyclopropyl, phenyl, difluorophenyl, and hydroxyphenyl.

(123) Exemplary compounds of the invention have a formula selected from C201, C208, C213, C216, C220, C221, C222, C223, and salts, esters, or prodrugs thereof. These compounds are depicted in Table 9 herein below.

(124) TABLE-US-00009 TABLE 9 Synthesized PAI-1 Inhibitor Compounds PAI-1/ uPA IC.sub.50 in buffer No. Structure (μM) C201 03 181 C208 04 77.2 C213 05 C216 06 C220 07 116.46 C221 08 1608 C222 09 236 C223 0 124.6

(125) Comparative compounds that do not demonstrate PAI-1 inhibitory activity in buffer (40 mM HEPES, 100 mM NaCl, 0.05% Tween-20, 10% DMSO, pH 7.8) in the assay described in Example 12 herein are depicted in Table 10.

(126) TABLE-US-00010 TABLE 10 Synthesized Comparative Compounds No. Structure E202 E209 E211 E212 E215 E217 E218 E219 E226

(127) Compounds of the invention include those of formula XIV or a salt, ester, or prodrug thereof:

(128) ##STR00220##

(129) wherein R is selected from the group consisting of phenyl and substituted biphenyl.

(130) Exemplary compounds of the invention have a formula selected from C199, C203, C206, C207, and salts, esters, or prodrugs thereof. These compounds are depicted in Table 11 herein below.

(131) TABLE-US-00011 TABLE 11 Synthesized PAI-1 Inhibitor Compounds PAI-1/ uPA IC.sub.50 in buffer No. Structure (μM) C199 5.36 C203 17.07 C206 4.96 C207 16.83

(132) Compounds of the invention include those of formula XV or a salt, ester, or prodrug thereof:

(133) ##STR00225##

(134) wherein:

(135) V is selected from the group consisting of (CH.sub.2).sub.n, C.sub.3 to C.sub.8 cycloalkyl, (CH.sub.2).sub.n—C.sub.3-C.sub.8 cycloalkyl-(CH.sub.2).sub.p, aryl, (CH.sub.2).sub.n-aryl-(CH.sub.2).sub.p, heteroaryl, (CH.sub.2).sub.n-heteroaryl-(CH.sub.2).sub.p,

(136) ##STR00226##
and substituted derivatives thereof;

(137) n and p are independently 0, 1, 2, 3, 4, 5, or 6;

(138) X.sub.1, X.sub.2, X.sub.3, X.sub.4, X.sub.5 and X.sub.6 are independently selected from the group consisting of —H, —OH, —OR, —F, —Cl, —Br, —I, —NO.sub.2, —NO, —N(R).sub.2, —N(R).sub.3.sup.+, —C(O)R, —C(O)OR, —CHO, —C(O)NH.sub.2, —C(O)SR, —CN, —S(O).sub.2R, —SO.sub.3R, —SO.sub.3H, —SO.sub.2N(R).sub.2, —S═O, C.sub.1 to C.sub.12 alkyl, aryl, substituted aryl, heteroaryl, and substituted heteroaryl;

(139) R is selected from the group consisting of C.sub.1 to C.sub.6 alkyl, C.sub.3 to C.sub.6 cycloalkyl, CH.sub.2—C.sub.3-C.sub.6 cycloalkyl, phenyl, tolyl, and benzyl;

(140) Y.sub.1 is selected from the group consisting of O, NH, NR.sup.a, S, and CH.sub.2;

(141) Y.sub.2 is selected from the group consisting of O, NH, NR.sup.b, S, and CH.sub.2;

(142) R.sup.a and R.sup.b are independently selected from the group consisting of C.sub.1 to C.sub.12 alkyl, C.sub.3 to C.sub.6 cycloalkyl, (CH.sub.2).sub.m—C.sub.3-C.sub.6 cycloalkyl, C.sub.2 to C.sub.6 heterocycloalkyl, (CH.sub.2).sub.m—C.sub.2-C.sub.6 heterocycloalkyl, benzyl, aryl, (CH.sub.2).sub.m-aryl, heteroaryl, (CH.sub.2).sub.m-heteroaryl, and substituted derivatives thereof;

(143) m is 1, 2, 3, 4, 5, or 6; and

(144) Z.sub.1, Z.sub.2, Z.sub.3, and Z.sub.4 are independently selected from the group consisting of C, P—OH, S, and S═O.

(145) Compounds of the invention include those of formula XVI or a salt, ester, or prodrug thereof:

(146) ##STR00227##
wherein V, n, p, X.sub.1, X.sub.2, X.sub.3, X.sub.4, X.sub.5, X.sub.6, Y.sub.1, Y.sub.2, R.sup.a, R.sup.b, Z.sub.1, Z.sub.2, Z.sub.3, and Z.sub.4 are as defined above for formula XV.

(147) Compounds of the invention include those of formula XVII or a salt, ester, or prodrug thereof:

(148) ##STR00228##

(149) wherein:

(150) X.sub.1, X.sub.2, X.sub.3, X.sub.4, X.sub.5 and X.sub.6 are independently selected from the group consisting of —H, —OH, —OR, —F, —Cl, —Br, —I, —NO.sub.2, —NO, —N(R).sub.2, —N(R).sub.3.sup.+, —C(O)R, —C(O)OR, —CHO, —C(O)NH.sub.2, —C(O)SR, —CN, —S(O).sub.2R, —SO.sub.3R, —SO.sub.3H, —SO.sub.2N(R).sub.2, —S═O, C.sub.1 to C.sub.12 alkyl, aryl, substituted aryl, heteroaryl, and substituted heteroaryl;

(151) R is selected from the group consisting of C.sub.1 to C.sub.6 alkyl, C.sub.3 to C.sub.6 cycloalkyl, CH.sub.2—C.sub.3-C.sub.6 cycloalkyl, phenyl, tolyl, and benzyl;

(152) R.sup.b is selected from the group consisting of H, C.sub.1 to C.sub.12 alkyl, C.sub.3 to C.sub.6 cycloalkyl, (CH.sub.2).sub.m—C.sub.3-C.sub.6 cycloalkyl, C.sub.2 to C.sub.6 heterocycloalkyl, (CH.sub.2).sub.m—C.sub.2-C.sub.6 heterocycloalkyl, benzyl, aryl, (CH.sub.2).sub.m-aryl, heteroaryl, (CH.sub.2).sub.m-heteroaryl, and substituted derivatives thereof;

(153) m is 1, 2, 3, 4, 5, or 6; and

(154) Z.sub.4 is selected from the group consisting of C, P—OH, S, and S═O.

(155) Compounds of the invention include those of formula XVIII or a salt, ester, or prodrug thereof:

(156) ##STR00229##
wherein X.sub.1, X.sub.2, X.sub.3, X.sub.4, X.sub.5, X.sub.6, R.sup.b, and Z.sub.4 are as defined above for formula XVII.

(157) In some embodiments, compounds of the invention include those of formula XVII or XVIII as defined above wherein X.sub.1 and X.sub.2 are independently selected from —OH and —OR.

(158) Compounds of the invention include those of formula XIX or a salt, ester, or prodrug thereof:

(159) ##STR00230##

(160) wherein:

(161) X.sub.3, X.sub.4, and X.sub.6 are independently selected from the group consisting of —H, —OH, —OR, —F, —Cl, —Br, —I, —NO.sub.2, —NO, —N(R).sub.2, —N(R).sub.3.sup.+, —C(O)R, —C(O)OR, —CHO, —C(O)NH.sub.2, —C(O)SR, —CN, —S(O).sub.2R, —SO.sub.3R, —SO.sub.3H, —SO.sub.2N(R).sub.2, —S═O, aryl, substituted aryl, heteroaryl, and substituted heteroaryl;

(162) R is selected from the group consisting of C.sub.1 to C.sub.6 alkyl, C.sub.3 to C.sub.6 cycloalkyl, CH.sub.2—C.sub.3-C.sub.6 cycloalkyl, phenyl, tolyl, and benzyl;

(163) Y.sub.1 is selected from the group consisting of CHR.sup.aR.sup.b, OR.sup.a, NHR.sup.a, NR.sup.aR.sup.b, and SR.sup.a;

(164) R.sup.a and R.sup.b are independently selected from the group consisting of C.sub.1 to C.sub.12 alkyl, C.sub.3 to C.sub.6 cycloalkyl, (CH.sub.2).sub.m—C.sub.3-C.sub.6 cycloalkyl, C.sub.2 to C.sub.6 heterocycloalkyl, (CH.sub.2).sub.m—C.sub.2-C.sub.6 heterocycloalkyl, benzyl, aryl, (CH.sub.2).sub.m-aryl, heteroaryl, (CH.sub.2).sub.m-heteroaryl, —U, (CH.sub.2).sub.m—U, and substituted derivatives thereof, or R.sup.a and R.sup.b taken together with the N atom to which they are bonded form a 3- to 8-membered heterocyclic ring;

(165) U is selected from the group consisting of —NR.sup.cR.sup.d, —NR.sup.cC(O)R.sup.e, —NR.sup.cC(O)OR.sup.e, —NR.sup.cC(O)NR.sup.fR.sup.e, —NR.sup.cC(O)SR.sup.e, —NR.sup.cP(O)(OH)R.sup.e, —NR.sup.cP(O)(OH)OR.sup.e, —NR.sup.cP(O)(OH)NR.sup.fR.sup.e, —NR.sup.cP(O)(OH)SR.sup.e, —NR.sup.cS(O)R.sup.e, —NR.sup.cS(O)OR.sup.e, —NR.sup.cS(O)NR.sup.fR.sup.e, —NR.sup.cS(O)SR.sup.e, —NR.sup.cS(O).sub.2R.sup.e, —NR.sup.cS(O).sub.2OR.sup.e, —NR.sup.cS(O).sub.2NR.sup.fR.sup.e, —NR.sup.cS(O).sub.2SR.sup.e, —OR.sup.f, —OC(O)R.sup.e, —OC(O)OR.sup.e, —OC(O)NR.sup.fR.sup.e, —OC(O)SR.sup.e, —OP(O)(OH)R.sup.e, —OP(O)(OH)OR.sup.e, —OP(O)(OH)NR.sup.dR.sup.e, —OP(O)(OH)SR.sup.e, —OS(O)R.sup.e, —OS(O)OR.sup.e, —OS(O)NR.sup.dR.sup.e, —OS(O)SR.sup.e, —OS(O).sub.2R.sup.e, —OS(O).sub.2OR.sup.e, —OS(O).sub.2NR.sup.dR.sup.e, —OS(O).sub.2SR, —C(O)OR.sup.c, —C(O)NR.sup.cR.sup.d, —C(O)SR.sup.c, and —C(O)R.sup.c;

(166) R.sup.c and R.sup.d are independently selected from the group consisting of H, C.sub.1 to C.sub.12 alkyl, C.sub.1 to C.sub.12 haloalkyl, C.sub.3 to C.sub.6 cycloalkyl, (CH.sub.2).sub.m—C.sub.3-C.sub.6 cycloalkyl, C.sub.2 to C.sub.6 heterocycloalkyl, (CH.sub.2).sub.m—C.sub.2-C.sub.6 heterocycloalkyl, benzyl, aryl, (CH.sub.2).sub.m-aryl, heteroaryl, (CH.sub.2).sub.m-heteroaryl, and substituted derivatives thereof, or R.sup.c and R.sup.d taken together with the N atom to which they are bonded form a 3- to 8-membered heterocyclic ring;

(167) R.sup.e, R.sup.f, and R.sup.g are independently selected from the group consisting of H, C.sub.1 to C.sub.12 alkyl, C.sub.1 to C.sub.12 haloalkyl, C.sub.3 to C.sub.6 cycloalkyl, (CH.sub.2).sub.m—C.sub.3-C.sub.6 cycloalkyl, C.sub.2 to C.sub.6 heterocycloalkyl, (CH.sub.2).sub.m—C.sub.2-C.sub.6 heterocycloalkyl, benzyl, aryl, (CH.sub.2).sub.m-aryl, heteroaryl, (CH.sub.2).sub.m-heteroaryl, and substituted derivatives thereof;

(168) m is 1, 2, 3, 4, 5, or 6; and

(169) Z.sub.1 and Z.sub.4 are independently selected from the group consisting of C, P—OH, S, and S═O.

(170) Exemplary compounds of the invention have a formula selected from C225, C227, C228, C229, and salts, esters, or prodrugs thereof. These compounds are depicted in Table 12 herein below.

(171) TABLE-US-00012 TABLE 12 Synthesized PAI-1 Inhibitor Compounds PAI-1/ uPA IC.sub.50 in buffer No. Structure (μM) C225 163 C227 205 C228 14.8 C229 1288

(172) Comparative compounds that do not demonstrate PAI-1 inhibitory activity in buffer (40 mM HEPES, 100 mM NaCl, 0.05% Tween-20, 10% DMSO, pH 7.4) in the assay described in Example 11 herein are depicted in Table 13.

(173) TABLE-US-00013 TABLE 13 Synthesized Comparative Compounds No. Structure E230 E231 E315 E316 E317 E318 0

(174) Compounds for use in the methods of the invention include those of formula XX to XXIX or a salt, ester, or prodrug thereof. Compounds of formula XX to XXIX that demonstrate PAI-1 inhibitory activity are depicted in Table 14.

(175) TABLE-US-00014 TABLE 14 PAI-1 Inhibitor Compounds Com- pound No. Structure Supplier XX Chembridge XXI Synthon Labs XXII Vitas M Labs XXIII Chembridge XXIV Chembridge XXV Chembridge XXVI Chembridge XXVII Synthon Labs XXVIII Synthon Labs XXIX 0 Enamine

(176) Methods of Making Inhibitors of PAI-1 Activity

(177) The compounds of the present invention can be readily prepared according to the following reaction schemes or modification thereof using readily available starting materials, reagents and conventional synthetic procedures. It is also possible to make use of variants of these process steps, which in themselves are known to and well within the preparatory skill of the medicinal chemist.

(178) Derivatives of PAI-1 inhibitors are also included herein. Such derivatives include molecules modified by one or more water soluble polymer molecules, such as polyethylene glycol, or by the addition of polyamino acids, including fusion proteins (procedures for which are well-known in the art). Such derivatization may occur singularly or there may be multiple sites of derivatization.

(179) Primary High-Throughput Screen for PAL-1 Inhibition

(180) High-throughput screening was carried out using the protocol described below. All screening was performed in the Center for Chemical Genomics in the Life Sciences Institute at the University of Michigan. For assay validation, the Microsource Spectrum 2000 was screened both in HEPES-buffered saline (HBS) and in HBS containing 15 mg/mL BSA. All other libraries were screened in HBS with 15 mg/mL BSA only. Purified compound libraries screened were the NIH Clinical Collection, Chemical Methodologies Libraries Development (Boston University), Maybridge, Chembridge, ChemDiv, National Cancer Institute-Development Therapeutics Program Library, the Cayman Cannabinoid and Epigenetics collections, EMD Protein Kinase collection, and the Enzo Autophagy, Protease, Natural products, REDOX, and Wnt Pathway libraries. Together, these libraries totaled approximately 152,899 purified compounds. For primary screening, all reagents except for compounds were added using a Thermo Scientific Multidrop Combi.

(181) Briefly, 6 μL of 15 nM PAI-1 in 15 mg/mL BSA was added per well, followed by addition of 200 nL of compound using a Beckman Biomek FX with a pin-tool attachment liquid handling system. One compound was added per well, yielding a compound concentration of approximately 32 μM (3.2% DMSO) in the presence of PAI-1. Following a 15-minute incubation, 3 μL of 15 nM uPA in HEPES-buffered saline (HBS) was added per well, for final concentrations of PAI-1 and uPA of 10 nM and 5 nM, respectively. A 2:1 PAI-1:uPA ratio was chosen to enrich for the most active compounds, as greater than half of the PAI-1 must be inactivated before a signal is generated. After an additional 15-minute incubation, 3 μL of the pNA/AMC substrate mixture was added in HBS to yield final concentrations of 200 μM and 100 μM, respectively. The mixture of uPA and substrates in the absence of PAI-1 served as a positive control, while the negative control consisted of the mixture uPA and substrates along with PAI-1. Following a 90-minute incubation to allow for substrate turnover, quenching of WPF by pNA (ex/em 430/470 nm) and AMC fluorescence (ex/em 380/470) were recorded using a BMG Labtech Pherastar plate reader. All data was analyzed using Tripos Benchware Dataminer.

(182) Using this HTS protocol, 152,899 purified compounds were screened from 15 different collections in the Center for Chemical Genomics (CCG) at the University of Michigan. Compounds were considered PAI-1 inactivators if a change in both the pNA and AMC signals was observed that was greater than three standard deviations (>3SD) from the negative control. Compounds that displayed a change in only one of the reporter signals were classified as false-positives and not considered for further evaluation. The average Z-factor values (Z′), which serve as a statistical gauge for the quality of the HTS assay, for pNA and AMC were 0.72 and 0.68, respectively, indicating that the pNA/AMC dual-reporter system is a statistically excellent assay.

(183) Because the pNA and AMC fluorescence reads were recorded individually, the data from each signal was also compared separately to explore the hit rates using pNA or AMC versus the dual-reporter system. Interestingly, a significantly high hit rate was observed for each reporter alone. Analysis of the AMC reporter alone revealed that 20.3% of compounds displayed a change in signal greater than 3SD from the negative control, exemplifying an high hit rate for a primary screen and portraying the difficulty in triaging compounds for confirmation and follow-up. For the pNA reporter only, the hit rate was lower relative to AMC, but also high at 8.7%. Upon applying both signals for the hit criteria, the overall hit rate was significantly reduced to 1.5% (2,363 compounds), indicating a reduction in the false-positive rate of 7.2-18.8%, depending upon which single reporter was used. Together, these HTS results demonstrate the usefulness and efficiency of applying a dual-reporter system for ruling out false-positives compared to a single reporter assay.

(184) Confirmation and Dose-Response Testing for PAI-1 Inhibition

(185) Confirmation testing for purified compounds was performed as described for the primary assay except that compounds were stamped in triplicate. In addition, a ‘pre-read’ was recorded after addition of PAI-1, compound and uPA, but prior to substrate mixture addition. Compounds that showed a change in pNA and AMC signals >3SD in at least 2 out of 3 wells were further examined. Of this subset, only compounds that showed <3SD change in signal in 2 or less wells relative to the negative control in the pre-read were chosen for dose-response testing. Based on these criteria, 300 compounds were chosen for dose-response testing in the CCG. For this analysis, varying volumes (29-600 nL) of compound were stamped in duplicate using a TTP Labtech Mosquito X1 liquid handling system, resulting in an approximate final concentration of 12-250 μM. Development was carried out as described for the primary screen.

(186) Methods of Using PAI-1 Inhibitors

(187) As mentioned herein above, it is contemplated that methods of the invention include treating a disease or disorder associated with elevated levels of PAI-1 comprising administering a PAI-1 inhibitor. In one aspect, the subject is a mammal. In a preferred aspect, the mammalian subject is human.

(188) In one embodiment, the invention includes PAI-1 inhibitor compounds and methods of using the compounds in the treatment of many diseases or disorders associated with PAI-1 activity. Such conditions, e.g., diseases or disorders, include, but are not limited to, dysregulation of lipid metabolism, obesity, diabetes, polycystic ovary syndrome, bone loss induced by estrogen deficiency, fibrosis and fibrotic disease, inflammation, cell migration and migration-driven proliferation of cells, and angiogenesis or thrombosis. In one aspect, such inhibitors are also contemplated to be useful for modulation of endogenous fibrinolysis, and in conjunction with pharmacologic thrombolysis. In another aspect, the invention includes PAI-1 inhibitor compounds and methods of using the compounds in the treatment of acute diseases associated with high PAI-1 levels, such as, but not limited to, sepsis, myocardial infarction, and thrombosis, compared to PAI-1 levels in normal subjects known not to suffer from sepsis, myocardial infarction, or thrombosis. In another aspect, the PAI-1 inhibitor compounds of the invention are used in methods for treating diseases and disorders associated with high PAI-1 levels, such as, but not limited to, cancer, atherosclerosis, insulin resistance, type 2 diabetes, and fibrotic diseases compared to PAI-1 levels in normal subjects known not to suffer from these diseases or disorders. In another aspect, the invention includes PAI-1 inhibitor compounds for regulating lipid metabolism, including increasing circulating HDL and/or decreasing circulating VLDL in a subject.

(189) In various aspects, a PAI-1 inhibitor is useful in the treatment of any condition, including a disease or disorder, wherein the lowering of PAI-1 levels will provide benefits. The PAI-1 inhibitor is useful alone, or in combination with other compounds, which may act as to promote the reduction of PAI-1 levels.

(190) One of the therapeutic embodiments of the invention is the provision, to a subject in need thereof, compositions comprising one or more PAI-1 inhibitor. In one aspect, the PAI-1 inhibitor is isolated from a known compound or is chemically synthesized. In another aspect, the PAI-1 inhibitor formulation for therapy in a subject is selected based on the route of administration and in certain aspects includes liposome and micelle formulations as well as classic pharmaceutical preparations.

(191) The PAI-1 inhibitor is formulated into an appropriate preparation and administered to one or more sites within the subject in a therapeutically effective amount. In one embodiment, the PAI-1 inhibitor-based therapy is effected via continuous or intermittent intravenous administration. In one aspect, the PAI-1 inhibitor-based therapy is effected via continuous or intermittent intramuscular or subcutaneous administration. In another aspect, the PAI inhibitor-based therapy is effected via oral or buccal administration. By “effective amount” the invention refers to an amount of PAI-1 inhibitor compound that is sufficient to support an observable change in the level of one or more biological activities of PAI-1, plasminogen activator, HDL, LDL, or VLDL and/or an observable change in an indication for which the method of treatment is intended. The change may be reduced level of PAI-1 activity. In one aspect, the change is an increase in plasminogen activator, and/or HDL and/or a reduction in LDL and VLDL.

(192) In various aspects, administration of the compositions is systemic or local, and in still other aspects comprises a single site injection of a therapeutically-effective amount of the PAI-1 inhibitor composition. Any route known to those of skill in the art for the administration of a therapeutic composition of the invention is contemplated including, for example, intravenous, intramuscular, subcutaneous, oral, or a catheter for long-term administration.

(193) Alternatively, it is contemplated that the therapeutic composition is delivered to the patient at multiple sites. The multiple administrations are rendered simultaneously or are administered over a period of several hours. It is likewise contemplated that the therapeutic composition is taken on a regular basis via oral administration. In certain cases, it is beneficial to provide a continuous flow of the therapeutic composition. Additional therapy is administered on a period basis, for example, daily, weekly, or monthly.

(194) In addition to therapies based solely on the delivery of the PAI-1 inhibitor composition, combination therapy is specifically contemplated. In the context of the invention, it is contemplated that the PAI-1 inhibitor composition therapy is used similarly in conjunction with other agents commonly used for the treatment of elevated levels of PAI-1, LDL and VLDL.

(195) To achieve the appropriate therapeutic outcome, using the methods and compositions of the invention, one would generally provide a composition comprising a PAI-1 inhibitor and at least one other therapeutic agent (second therapeutic agent). In one aspect of the invention, it is contemplated that methods include administration or inclusion of at least one additional factor or other drug. Such drugs include drugs used to manage cardiovascular disease including, but not limited to, cholesterol lowering drugs, such as statins, anti-inflammatories, and ACE inhibitors. Such drugs also include drugs targeting neurological disorders including, but not limited to drugs for targeting stroke, seizures, and Alzheimer's Disease. In another aspect, the additional drugs include, but are not limited to, drugs targeting diabetes. These are all disorders associated with elevated levels of PAI-1 and, therefore, it is contemplated that combination therapy may be used with PAI-1 inhibitors and other known therapies.

(196) The combination therapy compositions are provided in a combined amount effective to produce the desired therapeutic outcome in the treatment of increased levels of PAI-1, VLDL, or LDL and/or make a detectable change in an indication as described herein. This process involves administering the PAI-1 inhibitor and the second agent(s) or factor(s) at the same time. Methods thus include administering a single composition or pharmacological formulation that includes both agents, or administering two distinct compositions or formulations, at the same time, wherein one composition includes the PAI-1 inhibitor therapeutic composition and the other includes the second therapeutic agent.

(197) Alternatively, the PAI-1 inhibitor treatment precedes or follows the second therapeutic agent treatment by intervals ranging from minutes to weeks. In embodiments where the second therapeutic agent and the PAI-1 inhibitor are administered separately, one generally ensures that a significant period of time did not expire between the times of each delivery, such that the second therapeutic agent and the PAI-1 inhibitor are able to exert an advantageously combined effect. In such instances, it is contemplated that one administers both modalities within about 12-24 hours of each other, or alternately, within about 6-12 hours of each other, or alternately, with a delay time of only about 12 hours. In some situations, it is desirable to extend the time period for treatment significantly, however, where several days (2, 3, 4, 5, 6 or 7) to several weeks (1, 2, 3, 4, 5, 6, 7 or 8) lapse between the respective administrations.

(198) Systemic delivery of PAI-1 inhibitors to patients is a very efficient method for delivering a therapeutically effective amount of the compound to counteract the immediate clinical manifestations of a disease or disorder. Alternatively, local delivery of the PAI-1 inhibitor and/or the second therapeutic agent is appropriate in certain circumstances. In a certain embodiment, it is contemplated that the PAI-1 inhibitor is delivered to a patient for an extended period of time. It is further contemplated that the PAI-1 inhibitor is taken throughout a patient's lifetime to lower PAI-1, VLDL and/or LDL levels.

(199) Pharmaceutical Compositions

(200) As mentioned herein above, the invention also comprehends methods using pharmaceutical compositions comprising effective amounts of PAI-1 inhibitor together with pharmaceutically acceptable diluents, preservatives, solubilizers, emulsifiers, adjuvants and/or carriers useful in PAI-1 inhibitor therapy. Such compositions include diluents of various buffer content (e.g., Tris-HCl, acetate, phosphate), pH and ionic strength; additives such as detergents and solubilizing agents (e.g., Tween 80, Polysorbate 80), anti-oxidants (e.g., ascorbic acid, sodium metabisulfite), preservatives (e.g., thimersol, benzyl alcohol), and bulking substances (e.g., lactose, mannitol); incorporation of the material into particulate preparations of polymeric compounds, such as polylactic acid, polyglycolic acid, etc., or in association with liposomes or micelles. Such compositions will influence the physical state, stability, rate of in vivo release, and rate of in vivo clearance of the PAI-1 inhibitor. See, e.g., Remington's Pharmaceutical Sciences, 18th Ed. (1990) Mack Publishing Co., Easton, Pa., pages 1435-1712, which are herein incorporated by reference.

(201) Sterile liquid compositions include solutions, suspensions, emulsions, syrups and elixirs. The compounds of this invention may be dissolved or suspended in the pharmaceutically acceptable carrier, such as sterile water, sterile organic solvent or a mixture of both. In one aspect, the liquid carrier is one suitable for parental injection. Where the compounds are sufficiently soluble they can be dissolved directly in normal saline with or without the use of suitable organic solvents, such as propylene glycol or polyethylene glycol. If desired, dispersions of the finely divided compounds can be made-up in aqueous starch or sodium carboxymethyl cellulose solution, or in a suitable oil, such as arachis oil. Liquid pharmaceutical compositions, which are sterile solutions or suspensions, can be utilized by intramuscular, intraperitoneal or subcutaneous injection. In many instances a liquid composition form may be used instead of the preferred solid oral method of administration.

(202) It is preferred to prepare unit dosage forms of the compounds for standard administration regimens. In this way, the composition can be subdivided readily into smaller doses at the physician's direction. For example, unit dosages may be made up in packeted powders, vials or ampoules and, in one aspect, in capsule or tablet form. The active compound present in these unit dosage forms of the composition may be present in an amount of from about one gram to about fifteen grams or more, for single or multiple daily administration, according to the particular need of the patient. The daily dose of active compound will vary depending upon the route of administration, the size, age and sex of the patient, the severity of the disease state, and the response to the therapy as traced by blood analysis and the patient's recovery rate.

(203) The precise dosage to be employed depends upon several factors including the host, whether in veterinary medicine or human medicine, the nature and severity of the condition, e.g., disease or disorder, being treated, the mode of administration and the particular active substance employed. The compounds may be administered by any conventional route, in particular enterally, and, in one aspect, orally in the form of tablets or capsules. Administered compounds can be in the free form or pharmaceutically acceptable salt form as appropriate, for use as a pharmaceutical, particularly for use in the prophylactic or curative treatment of atherosclerosis and sequelae (angina pectoris, myocardial infarction, arrhythmias, heart failure, kidney failure, stroke, peripheral arterial occlusion, and related disease states). These measures will slow the rate of progress of the disease state and assist the body in reversing the process direction in a natural manner.

(204) PAI-1 inhibitors or derivatives thereof may be formulated for injection, or oral, nasal, pulmonary, topical, or other types of administration as one skilled in the art will recognize. The formulation may be liquid or may be solid, such as lyophilized, for reconstitution.

(205) PAI-1 inhibitor or derivatives thereof are useful in the treatment of any of the acute or chronic diseases or disorders associated with increased levels of PAI-1, LDL, or VLDL. Conditions (e.g., diseases or disorders) alleviated or modulated by the administration of PAI-1 inhibitor, in some aspects, are those characterized by increased levels of VLDL and LDL. Such conditions may be induced as a course of therapy for other purposes, such as chemotherapy or radiation therapy. It is contemplated that such conditions may result from genetic inheritance or be the side effect of another condition or medication.

(206) The phrase “pharmaceutically or pharmacologically acceptable” refers to molecular entities and compositions that do not produce adverse, allergic, or other untoward reactions when administered to an animal or a human. As used herein, “pharmaceutically acceptable carrier” includes any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents and the like. The use of such media and agents for pharmaceutically active substances is well-known in the art. Except insofar as any conventional media or agent is incompatible with the vectors or cells of the invention, its use in therapeutic compositions is contemplated. Supplementary active ingredients also can be incorporated into the compositions.

(207) The active compositions used in the methods of the invention include classic pharmaceutical preparations. Administration of these compositions according to the invention will be via any common route so long as the target tissue is available via that route. The pharmaceutical compositions may be introduced into the subject by any conventional method, e.g., by intravenous, intradermal, intramusclar, intramammary, intraperitoneal, intrathecal, retrobulbar, intrapulmonary (e.g., term release); by oral, sublingual, nasal, anal, vaginal, or transdermal delivery, or by surgical implantation at a particular site. The treatment may consist of a single dose or a plurality of doses over a period of time.

(208) The active compounds may be prepared for administration as solutions of free base or pharmacologically acceptable salts in water suitably mixed with a surfactant, such as hydroxypropylcellulose. Dispersions also can be prepared in glycerol, liquid polyethylene glycols, and mixtures thereof and in oils. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of microorganisms.

(209) The pharmaceutical forms suitable for injectable use include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. In all cases, the form must be sterile and must be fluid to the extent that easy syringability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms, such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol, and the like), suitable mixtures thereof, and vegetable oils. The proper fluidity can be maintained, for example, by the use of a coating, such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. The prevention of the action of microorganisms can be brought about by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, thimerosal, and the like. In many cases, it will be preferable to include isotonic agents (for example, sugars or sodium chloride). Prolonged absorption of the injectable compositions can be brought about by the use in the compositions of agents delaying absorption (for example, aluminum monostearate and gelatin).

(210) Sterile injectable solutions are prepared by incorporating the active compounds in the required amount in the appropriate solvent with several of the other ingredients enumerated above, as required, followed by filtered sterilization. Generally, dispersions are prepared by incorporating the various sterilized active ingredients into a sterile vehicle that contains the basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum-drying and freeze-drying techniques that yield a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof.

(211) As used herein, “pharmaceutically acceptable carrier” includes any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents and the like. The use of such media and agents for pharmaceutical active substances is well-known in the art. Except insofar as any conventional media or agent is incompatible with the active ingredient, its use in the therapeutic compositions is contemplated. Supplementary active ingredients also can be incorporated into the compositions.

(212) For oral administration of the compositions used in the methods of the invention, a PAI-1 inhibitor may be incorporated with excipients and used in the form of non-ingestible mouthwashes and dentifrices. A mouthwash may be prepared incorporating the active ingredient in the required amount in an appropriate solvent, such as a sodium borate solution (Dobell's Solution). Alternatively, the active ingredient may be incorporated into an antiseptic wash containing sodium borate, glycerin and potassium bicarbonate. The active ingredient may also be dispersed in dentifrices, including: gels, pastes, powders and slurries. The active ingredient may be added in a therapeutically effective amount to a paste dentifrice that may include water, binders, abrasives, flavoring agents, foaming agents, and humectants.

(213) The compositions used in the methods of the invention may be formulated in a neutral or salt form. Pharmaceutically-acceptable salts include the acid addition salts (formed with the free amino groups of the protein) and which are formed with inorganic acids such as, for example, hydrochloric or phosphoric acids, or such organic acids as acetic, oxalic, tartaric, mandelic, and the like. Salts formed with the free carboxyl groups also can be derived from inorganic bases such as, for example, sodium, potassium, ammonium, calcium, or ferric hydroxides, and such organic bases as isopropylamine, trimethylamine, histidine, procaine and the like.

(214) The compositions used in the methods of the invention may be formulated in micelles or liposomes. Such formulations include sterically stabilized micelles or liposomes and sterically stabilized mixed micelles or liposomes. Such formulations can facilitate intracellular delivery, since lipid bilayers of liposomes and micelles are known to fuse with the plasma membrane of cells and deliver entrapped contents into the intracellular compartment.

(215) Upon formulation, solutions will be administered in a manner compatible with the dosage formulation and in such amount as is therapeutically effective. The formulations are easily administered in a variety of dosage forms such as injectable solutions, drug release capsules and the like. For parenteral administration in an aqueous solution, for example, the solution should be suitably buffered if necessary and the liquid diluent first rendered isotonic with sufficient saline or glucose. These particular aqueous solutions are especially suitable for intravenous, intramuscular, subcutaneous and intraperitoneal administration.

(216) Generally, an effective amount of a PAI-1 inhibitor, or derivatives thereof, will be determined by the age, weight, and condition or severity of disease or disorder of the recipient. See, Remington's Pharmaceutical Sciences, supra, pages 697-773, herein incorporated by reference. Typically, a dosage of between about 0.001 μg/kg body weight/day to about 1000 μg/kg body weight/day, may be used, but more or less, as a skilled practitioner will recognize, may be used. Dosing may be one or more times daily, or less frequently, and may be in conjunction with other compositions as described herein. It should be noted that the invention is not limited to the dosages recited herein.

(217) By initiating the treatment regimen with a minimal daily dose of about one gram, the blood levels of PAI-1 and the patient's symptomatic relief analysis may be used to determine whether a larger dose is indicated. One skilled in the art will appreciate that the appropriate dosage levels for treatment will thus vary depending, in part, upon the molecule delivered, the indication for which the PAI-1 inhibitor compound is being used, the route of administration, and the size (body weight, body surface or organ size) and condition (the age and general health) of the patient. Accordingly, the clinician may titer the dosage and may modify the route of administration to obtain the optimal therapeutic effect. A typical dosage may range from about 0.1 μg/kg to up to about 100 mg/kg or more, depending on the factors mentioned above. In other embodiments, the dosage may range from 0.1 μg/kg up to about 100 mg/kg; or 1 μg/kg up to about 100 mg/kg; or 5 μg/kg up to about 100 mg/kg.

(218) “Unit dose” is defined as a discrete amount of a therapeutic composition dispersed in a suitable carrier. Parenteral administration may be carried out with an initial bolus followed by continuous infusion to maintain therapeutic circulating levels of drug product. Those of ordinary skill in the art will readily optimize effective dosages and administration regimens as determined by good medical practice and the clinical condition of the individual patient.

(219) The frequency of dosing will depend on the pharmacokinetic parameters of the agents and the routes of administration. The optimal pharmaceutical formulation will be determined by one of skill in the art depending on the route of administration and the desired dosage. See, for example, Remington's Pharmaceutical Sciences, supra, pages 1435-1712, incorporated herein by reference. Such formulations may influence the physical state, stability, rate of in vivo release and rate of in vivo clearance of the administered agents. Depending on the route of administration, a suitable dose may be calculated according to body weight, body surface areas or organ size. Further refinement of the calculations necessary to determine the appropriate treatment dose is routinely made by those of ordinary skill in the art without undue experimentation, especially in light of the dosage information and assays disclosed herein, as well as the pharmacokinetic data observed in animals or human clinical trials.

(220) Appropriate dosages may be ascertained through the use of established assays for determining level of myocardial infarct in conjunction with relevant dose-response data. The final dosage regimen will be determined by the attending physician, considering factors that modify the action of drugs, e.g., the drug's specific activity, severity of the damage and the responsiveness of the patient, the age, condition, body weight, sex and diet of the patient, the severity of any infection, time of administration and other clinical factors. As studies are conducted, further information will emerge regarding appropriate dosage levels and duration of treatment.

(221) It will be appreciated that the pharmaceutical compositions and treatment methods of the invention are useful in fields of human medicine and veterinary medicine. Thus the subject to be treated is in one aspect a mammal. In another aspect, the mammal is a human.

(222) In addition, the invention contemplates a kit containing components comprising a composition comprising a PAI-1 inhibitor; and optionally, at least one additional factor useful in the treatment of the acute and chronic diseases and disorders discussed herein.

(223) Uses of Compounds of the Invention in the Treatment of Diseases or Disorders

(224) The invention includes the use of compounds of the invention for the production of a medicament for the treatment or prevention of any disease or disorder discussed herein.

(225) The compounds of the invention are inhibitors of the serine protease inhibitor PAI-1, and are therefore useful in the treatment or prophylaxis of those processes which involve the production and/or action of PAI-1. Thus, the compounds of the invention, in various aspects, are useful in preventing or reducing thrombosis, promoting thrombolysis, reducing fibrosis regulating lipid metabolism as described herein. In one aspect, the compounds of the invention are useful in treating high cholesterol and diseases or disorders associated with elevated levels of PAI-1. In another aspect, the compounds of the invention are useful in treating elevated levels of VLDL or LDL. In another aspect, the compounds of the invention are useful in elevating HDL.

(226) In one aspect, the invention includes the uses of these inhibitors for the treatment of many diseases or disorders associated with PAI-1 activity. Such diseases or disorders include, but are not limited to, inflammation, cell migration and migration-driven proliferation of cells, and angiogenesis or thrombosis. Such inhibitors are also contemplated to be useful for modulation of endogenous fibrinolysis, and in conjunction with pharmacologic thrombolysis.

(227) The compounds of the invention are useful in the treatment or prevention of insulin resistance, obesity, non-insulin dependent diabetes mellitus, cardiovascular disease, thrombotic events associated with coronary artery and cerebrovascular disease. The compounds of the invention are also useful for inhibiting the disease process involving the thrombotic and prothrombotic states which include, but are not limited to, formation of atherosclerotic plaques, venous and arterial thrombosis, myocardial ischemia, atrial fibrillation, deep vein thrombosis, coagulation syndromes, pulmonary thrombosis, cerebral thrombosis, thromboembolic complications of surgery (such as joint replacement), and peripheral arterial occlusion. These compounds are also useful in treating stroke associated with or resulting from atrial fibrillation.

(228) The compounds of the invention are also used in the treatment or prophylaxis of high cholesterol and diseases or disorders associated with such a condition.

(229) The compounds of the invention may also be used in the treatment of diseases or disorders associated with extracellular matrix accumulation, including, but not limited to, renal fibrosis, chronic obstructive pulmonary disease, polycystic ovary syndrome, restenosis, renovascular disease and organ transplant rejection.

(230) The compounds of the invention may also be used in the treatment of malignancies, and diseases or disorders associated with neoangiogenesis (such as diabetic retinopathy).

(231) The compounds in the invention may also be used in conjunction with and following processes or procedures involving maintaining blood vessel patency, including vascular surgery, vascular graft and stent patency, organ, tissue and cell implantation and transplantation.

(232) The compounds of the invention may also be used in the treatment of Alzheimer's disease. This method may also be characterized as the inhibition of plasminogen activator by PAI-1 in a mammal, particularly a human, experiencing or subject to Alzheimer's disease. This method may also be characterized as a method of increasing or normalizing levels of plasmin concentration in a mammal, particularly those experiencing or subject to Alzheimer's disease.

(233) The compounds of the invention may be used for the treatment of myelofibrosis with myeloid metaplasia by regulating stromal cell hyperplasia and increases in extracellular matrix proteins.

(234) The compounds of the invention may also be used in conjunction with protease inhibitor-containing highly active antiretroviral therapy (HAART) for the treatment of diseases or disorders which originate from fibrinolytic impairment and hypercoagulability of HIV-1 infected patients receiving such therapy.

(235) The compounds of the invention may be used for the treatment of diabetic nephropathy and renal dialysis associated with nephropathy.

(236) The compounds of the invention may be used to treat cancer, septicemia, proliferative diseases, such as psoriasis, improving coagulation homeostasis, cerebrovascular diseases, microvascular disease, hypertension, dementia, atherosclerosis, osteoporosis, arthritis, asthma, heart failure, arrhythmia, angina, and as a hormone replacement agent, treating, preventing or reversing progression of atherosclerosis, Alzheimer's disease, osteoporosis, osteopenia; reducing inflammatory markers, fibrinolytic disorder, reducing C-reactive protein, or preventing or treating low grade vascular inflammation, stroke, dementia, coronary heart disease, primary and secondary prevention of myocardial infarction, stable and unstable angina, primary prevention of coronary events, secondary prevention of cardiovascular events, peripheral vascular disease, peripheral arterial disease, acute vascular syndromes, deep vein thrombosis, pulmonary embolism, reducing the risk of undergoing a myocardial revascularization procedure, microvascular diseases such as nephropathy, neuropathy, retinopathy and nephrotic syndrome, hypertension, Type 1 and 2 diabetes and related diseases, obesity, insulin resistance, hyperglycemia, hyperinsulinemia, malignant lesions, premalignant lesions, gastrointestinal malignancies, liposarcomas and epithelial tumors, proliferative diseases such as psoriasis, improving coagulation homeostasis, and/or improving endothelial function, and all forms of cerebrovascular diseases.

(237) The compounds of the invention may be used for the topical applications in wound healing for prevention of scarring.

(238) The compounds in the invention can be used in the treatment of inflammatory diseases, septic shock and the vascular damage associated with infections and for the treatment of blood and blood products used in dialysis, blood storage in the fluid phase, especially ex vivo platelet aggregation. The compounds in the present invention may also be used in combination with prothrombolytic, fibrinolytic and anticoagulant agents. The present compounds may also be added to human plasma during the analysis of blood chemistry in hospital settings to determine the fibrinolytic capacity thereof.

(239) This invention further comprises methods for treating, preventing, ameliorating or inhibiting each of the maladies mentioned herein in a mammal, in one aspect, in a human, the method(s) each comprising administering to a mammal in need of such treatment, prevention, amelioration or inhibition a pharmaceutically or therapeutically effective amount of a compound of this invention, or a pharmaceutically acceptable salt, ester, or prodrug form thereof.

(240) The compounds of the invention may also be used to treat cancer including, but not limited to, breast and ovarian cancer, and as imaging agents for the identification of metastatic cancers.

(241) It will be understood that a pharmaceutically or therapeutically effective amount of a compound herein refers to an amount of the compound in question which will sufficiently inhibit the serine protease inhibitor PAI-1 in the mammal in need thereof to a sufficient extent to provide a desirable improvement in the condition in question or provide sufficient inhibition of the serine protease inhibitor PAI-1 to prevent, inhibit or limit the onset of the physiological basis for the malady or condition in question.

EXAMPLES

(242) The invention is described in more detail with reference to the following non-limiting examples, which are offered to more fully illustrate the invention, but are not to be construed as limiting the scope thereof. Those of skill in the art will understand that the techniques described in these examples represent techniques described by the inventors to function well in the practice of the invention, and as such constitute preferred modes for the practice thereof. However, it should be appreciated that those of skill in the art should in light of the present disclosure, appreciate that many changes can be made in the specific methods that are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention.

(243) As discussed herein above, elevated levels of PAI-1 have been implicated in a variety of diseases and disorders. The development of therapeutic agents that act as selective inhibitors of PAI-1 may provide an approach to treat these diseases and disorders. The design and synthesis of a variety of compounds and their structure:activity relationship with PAI-1 is described. Additional synthetic methods for obtaining PAI-1 inhibitors are disclosed in US 2010/0137194, which is incorporated by reference in its entirety.

Example 1—Synthesis of Compounds of Formula III

(244) General Procedure A

(245) Compounds of formula III were synthesized according to the procedure described below and as shown in the following scheme:

(246) ##STR00251##

(247) To a 0° C. solution of an appropriately-substituted carboxylic acid (1 equiv.), an appropriately-substituted amine (1.2 equiv.), N-methylmorpholine (1 equiv.), and HOBT.H.sub.2O (1 equiv.) in 2.5:1 dry CH.sub.2Cl.sub.2 and DMF, EDC.HCl (1 equiv.) was added in portions. The resulting slurry was allowed to warm to room temperature overnight with stirring. The reaction mixture was concentrated by rotary evaporation, diluted with a 4:1 solution of EtOAc and hexane, washed with 0.1N HCl (2×), saturated NaHCO3 (2×), and brine (2×). The organic phase was dried with anhydrous MgSO.sub.4 and concentrated in vacuo. The resultant solid was triturated with chloroform and the solid obtained after filtration was dried in vacuo, which afforded compounds of formula III.

Synthesis of N-(4-chloro-3-(trifluoromethyl)benzyl)-3-(3,4-dihydroxyphenyl)propanamide (compound C330)

(248) N-(4-chloro-3-(trifluoromethyl)benzyl)-3-(3,4-dihydroxyphenyl)propanamide (compound C330) was synthesized according to General Procedure A. To a 0° C. solution of 3,4-dihydroxyhydro cinnamic acid (205.8 mg, 1.13 mmol), 4-chloro-3-(trifluoromethyl)benzylamine (0.2 mL, 1.36 mmol), N-methylmorpholine (0.12 mL, 1.13 mmol) and HOBT.H.sub.2O (173.0 mg, 1.13 mmol) in 5 mL of dry CH.sub.2Cl.sub.2 and 2 mL of DMF, EDC.HCl (216.6 mg, 1.13 mmol) was added in portions. The resulting slurry was allowed to warm to room temperature overnight with stirring. The reaction mixture was concentrated by rotary evaporation, diluted with 30 mL of a 4:1 solution of EtOAc and hexane, washed with 0.1N HCl (2×), saturated NaHCO3 (2×), and brine (2×). The organic phase was dried with anhydrous MgSO.sub.4 and concentrated in vacuo. The resultant solid was triturated with chloroform and the solid obtained after filtration was dried in vacuo, which afforded 235 mg (48%) of product as a pale yellow crystalline solid. .sup.1H NMR (DMSO-d.sub.6, 400 MHz) δ 8.66 (s, 1H), 8.59 (s, 1H), 8.35 (t, J=6.0 Hz, 1H), 7.66 (s, 1H), 7.57 (d, J=8.2 Hz, 1H), 7.31 (d, J=8.2 Hz, 1H), 6.57 (d, J=8.2 Hz, 1H), 6.54 (d, J=1.8 Hz, 1H), 6.39 (dd, J=1.8, 8.2 Hz, 1H), 4.27 (d, J=6.0 Hz, 2H), 2.62 (t, J=7.8 Hz, 2H), 2.33 (t, J=7.7 Hz, 2H). .sup.13C NMR (DMSO-d.sub.6, 100 MHz) δ 172.32, 145.53, 143.89, 140.46, 133.24, 132.45, 131.99, 129.25, 126.99 (q, J=5.7 Hz), 126.53 (q, J=30.5 Hz), 125.96, 123.41 (q, J=270.8 Hz), 119.30, 116.29, 115.92, 41.54, 37.90. HRMS, DART calcd. for C.sub.17H.sub.15F.sub.3NO.sub.3Cl [M+H].sup.+ 374.07707. found: 374.07880.

(249) The following compounds also were synthesized according to General Procedure A: C279, C286, C330, C344, C345, C346, C347, C348, C356, C357, C358, C359, C360, C361, C363, C364.

Synthesis of N-(4-chloro-3-(trifluoromethyl)benzyl)-5-oxo-4,5-dihydro-1,3,4-oxadiazole-2-carboxamide (compound C285)

(250) N-(4-Chloro-3-(trifluoromethyl)benzyl)-5-oxo-4,5-dihydro-1,3,4-oxadiazole-2-carboxamide (C285) was synthesized according to the procedure described below and as shown in the following scheme:

(251) ##STR00252##

(252) To a solution of compound C251 (0.125 g, 0.422 mmol) in 6 mL tetrahydrofuran was added 1,1′-carbonyldiimidazole (0.082 g, 0.506 mmol). The mixture was allowed to stir for 18 hr at room temperature, at which time the reaction was quenched with 1N HCl, taken up in 30 mL ethyl acetate, and washed with brine. The organic layer was dried, filtered, and concentrated in vacuo. The resulting residue was triturated with chloroform and filtered to provide 0.087 g (64%) of product (compound C285) as a solid.

(253) Compounds C299 and C306 were prepared according to above procedure except that compound C282 and compound C305, respectively, were used in place of compound C251.

Example 2—Synthesis of Compounds of Formula IV

(254) General Procedure B

(255) Compounds of formula IV were synthesized according to the procedure described below and as shown in the following scheme:

(256) ##STR00253##

(257) Step 1: To a 0° C. solution of triphosgene (1 equiv.) in dichloromethane, an appropriately-substituted amine (2.5 equiv.) was added dropwise with stirring. A solution of triethylamine (4.7 equiv.) in dichloromethane was then added. The mixture was allowed to stir for 5 min at 0° C., then overnight at 25° C. The reaction mixture was concentrated in vacuo, triturated with ethyl acetate, and filtered. The filtrate was concentrated in vacuo to provide the isocyanate product.

(258) Step 2: An ethanolic solution of hydroxylamine (1 equiv.) was added to a −60° C. solution of an appropriately-substituted isocyanate (1 equiv.) in dry dichloromethane. The mixture was allowed to warm to room temperature and stir for 18 hr. The reaction solution was chilled in an ice bath and filtered cold. The filtrate was concentrated in vacuo and the resulting residue was taken up in ethyl acetate and washed with brine (lx). The organic layer was separated, dried, filtered, and concentrated in vacuo. The resulting residue was triturated with chloroform and filtered to provide compounds of formula IV.

Synthesis of 4-chloro-3-trifluoromethylbenzyl isocyanate

(259) To a 0° C. solution of triphosgene (0.630 g, 2.12 mmol) in 10 mL dichloromethane, 4-chloro-3-trifluoromethylbenzyl amine (0.800 mL, 5.23 mmol) was added dropwise with stirring. A solution of triethylamine (1.39 mL, 10 mmol) in 5 mL dichloromethane was then added. A white precipitate formed immediately. The mixture was allowed to stir for 5 min at 0° C., then overnight at 25° C. The reaction mixture was concentrated in vacuo, triturated with ethyl acetate, and filtered. The filtrate was concentrated in vacuo to provide 1.19 g (97%) of 4-chloro-3-trifluoromethylbenzyl isocyanate as a clear oil.

Synthesis of 1-(4-chloro-3-(trifluoromethyl)benzyl)-3-hydroxyurea (compound C284)

(260) An ethanolic hydroxylamine solution was prepared by addition at 0° C. of a solution of 25 mmol NaOH in 55 mL absolute ethanol to a stirring suspension of 25 mmol hydroxylamine hydrochloride in 60 mL of absolute ethanol. 8.9 mL of the ethanolic solution of hydroxylamine (1.93 mmol) was added to a −60° C. solution of 4-chloro-3-trifluoromethylbenzyl isocyanate (0.455 g, 1.93 mmol) in 10 mL dry dichloromethane. The mixture was allowed to warm to room temperature and stir for 18 hr. The reaction solution was chilled in an ice bath and filtered cold. The filtrate was concentrated in vacuo and the resulting residue was taken up in 30 mL ethyl acetate and washed with brine (1×). The organic layer was separated, dried, filtered, and concentrated in vacuo. The resulting residue was triturated with chloroform and filtered to provide 0.140 g (27%) of compound C284 as a white solid.

Example 3—Synthesis of Compounds of Formula V

(261) General Procedure C

(262) Compounds of formula V were synthesized according to the procedure described below and as shown in the following scheme:

(263) ##STR00254##

(264) To a 0° C. solution of an appropriately-substituted amine (1 equiv.) and pyridine (3.7 equiv.) in dichloromethane, an appropriately-substituted chlorocarbonyl ester (1 equiv.) was added dropwise. The solution was removed from the ice bath and left to react for 30 minutes. The reaction mixture was washed with 0.2 N HCl (2×), saturated NaHCO.sub.3 (2×), and brine (2×), and was then dried with MgSO.sub.4, filtered and concentrated in vacuo to afford compounds of formula V.

Synthesis of ethyl 2-((4-chloro-3-(trifluoromethyl)benzyl)amino)-2-oxoacetate (compound C256)

(265) Ethyl 2-((4-chloro-3-(trifluoromethyl)benzyl)amino)-2-oxoacetate (compound C256) was synthesized according to General Procedure C. To a 0° C. solution of 4-chloro-3-trifluoromethylbenzylamine (187 μL, 1.23 mmol) and pyridine (370 μL, 4.6 mmol) in dichloromethane (6 ml), ethyl 2-chloro-2-oxoacetate (170 μL, 1.25 mmol) was added dropwise. The solution was removed from the ice bath and left to react for 30 minutes. The reaction mixture was washed with 0.2 N HCl (2×), saturated NaHCO.sub.3 (2×), and brine (2×), and was then dried with MgSO.sub.4, filtered and concentrated in vacuo to afford 0.381 g of compound C256 as a white solid (74%). .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 7.48 (m, 3H), 4.53 (d, J=6.4 Hz, 2H), 4.35 (q, J=7.3 Hz, 6.9 Hz, 2H), 1.38 (t, J=6.9 Hz, 3H); .sup.13C NMR (CDCl.sub.3, 100 MHz) δ 160.48, 156.87, 136.25, 132.48, 131.94, 131.85, 128.77 (q, J=31.5 Hz), 127.15 (q, J=4.8 Hz), 122.69 (q, J=271.7 Hz), 63.53, 42.89, 13.97.

(266) The following compounds also were synthesized according to General Procedure C: C256, C259, C265, C267, C276, C277, C288.

(267) General Procedure D

(268) Carboxylic acids were synthesized according to the procedure described below and as shown in the following scheme:

(269) ##STR00255##

(270) An appropriately-substituted ester (1 equiv.) was dissolved in a 2:1 solution of ethanol and water. 1.0 N NaOH (4 equiv.) was added to the reaction. After TLC analysis indicated that the starting material had been completely consumed, approximately 3 equivalents of 1.0 N HCl were used to quench the reaction. The reaction was cooled over ice as a precipitate formed and was then concentrated via filtration to afford the product carboxylic acid.

Synthesis of 2-((4-chloro-3-(trifluoromethyl)benzyl)amino)-2-oxoacetic acid (compound C309)

(271) 2-((4-Chloro-3-(trifluoromethyl)benzyl)amino)-2-oxoacetic acid (compound C309) was synthesized according to General Procedure D. Ethyl 2-((4-chloro-3-(trifluoromethyl)benzyl)amino)-2-oxoacetate (324.7 mg, 1.0 mmol) was dissolved in 12 ml of a 2:1 solution of ethanol and water. 4 ml of 1.0 N NaOH (4.0 mmol) was added to the reaction. After approximately 5 minutes, TLC analysis indicated that the starting material had been completely consumed. Approximately 3 equivalents of 1.0 N HCl (12 ml) were used to quench the reaction. The reaction was cooled over ice as a precipitate formed and was then concentrated via filtration to afford 143 mg (50%) of product as a white solid. .sup.1H NMR (DMSO-d.sub.6, 400 MHz) δ 9.42 (t, J=6.0 Hz, 1H), 7.65 (d, J=8.3 Hz, 1H), 7.73 (d, J=1.8 Hz, 1H), 7.54 (dd, J=8.3 Hz, 1H), 4.34 (d, J=6.44 Hz, 2H); .sup.13C NMR (DMSO-d.sub.6, 100 MHz) δ 162.38, 159.10, 139.26, 133.70, 132.15, 129.70 (q, J=1.9 Hz), 127.39 (q, J=4.8 Hz), 126.95 (q, J=30.5 Hz), 123.38 (q, J=271.7 Hz), 42.09. Percent Yield: 50.74%. HRMS, DART calcd. for C.sub.10H.sub.8ClF.sub.3NO.sub.3 282.01448. found: 282.01169.

(272) The following compound also was synthesized according to General Procedure D: C311.

Example 4—Synthesis of Compounds of Formula VI

(273) General Procedure E

(274) Compounds of formula VI were synthesized according to the procedure described below and as shown in the following scheme:

(275) ##STR00256##

(276) To a mixture of an appropriately-substituted carboxylic acid (1 equiv.), an appropriately-substituted amine (1.15 equiv.), 1-hydroxybenzotriazole [HOBt.H.sub.2O] (1.15 equiv.), N-methylmorpholine [NMM] (1.15 equiv.) in methylene chloride, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC.HCl) (1.15 equiv.) was added and stirred for overnight. The resulting solution was diluted with ethyl acetate and washed with 1N HCl (2×), saturated NaHCO.sub.3 (2×), and brine solution, dried with MgSO.sub.4, filtered, and concentrated in vacuo to obtain compounds of formula VI.

Synthesis of N-(4-chloro-3-(trifluoromethyl)benzyl)-N′-methoxyoxalamide (compound C320)

(277) N-(4-chloro-3-(trifluoromethyl)benzyl)-N′-methoxyoxalamide (compound C320) was synthesized according to General Procedure E. To a mixture of 2-((4-chloro-3-(trifluoromethyl)benzyl)amino)-2-oxoacetic acid (103.8 mg, 0.369 mmol), methoxyamine hydrochloride (36.9 mg, 0.426 mmol), 1-hydroxybenzotriazole [HOBt.H.sub.2O] (65.8 mg, 0.426 mmol), N-methylmorpholine [NMM] (47.0 μl, 0.426 mmol) in methylene chloride (4 ml), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC.HCl) (82.4 mg, 0.426 mmol) was added and stirred for overnight. The resulting solution was diluted with ˜20 ml of ethyl acetate and washed with 1N HCl (2×), saturated NaHCO.sub.3 (2×), and brine solution, dried with MgSO.sub.4, filtered, and concentrated in vacuo to obtain 0.0541 g (47.2%) of the product as a white solid. .sup.1H NMR (DMSO-d.sub.6, 400 MHz) δ 12.11 (bs, 1H), 9.45 (t, J=5.9 Hz, 1H), 7.73 (d, J=1.8 Hz, 1H), 7.65 (d, J=8.2 Hz, 1H), 7.53 (dd, J=6.8 Hz, J=1.4 Hz, 1H), 4.33 (d, J=6.4 Hz, 2H), 3.59 (s, 3H); .sup.13C NMR (DMSO-d.sub.6, 100 MHz) δ 160.17, 156.99, 139.26, 133.78, 132.16, 129.70, 127.52 (q, J=4.77 Hz), 126.92 (q, J=31.46 Hz), 223.38 (q, J=270.8 Hz), 63.67, 41.83; HRMS, DART calcd. for C.sub.11H.sub.1ClF.sub.3N.sub.2O.sub.3 [M+H].sup.+ 311.04103. found: 311.03891.

(278) The following compounds also were synthesized according to General Procedure E: C280, C300, C313, C314, C320, C323, C326, C328, C334, C342.

Example 5—Synthesis of Compounds of Formula VII

(279) General Procedure F

(280) Compounds of formula VII were synthesized according to the procedure described below and as shown in the following scheme:

(281) ##STR00257##

(282) An appropriately-substituted compound of formula V prepared according to General Procedure C in Example 3 (1 equiv.) was dissolved in ethanol. An appropriately-substituted aqueous hydrazine (2 equiv.) was then added dropwise. The mixture was allowed to stir at room temperature for 48 hours. The solution was filtered, which afforded compounds of formula VII.

(283) Synthesis of Compound C301

(284) Compound C301 was synthesized according to General Procedure F. Ethyl 2-(2,3-dichlorobenzylamino)-2-oxoacetate (88.5 mg, 0.32 mmol, according to General Procedure C) was dissolved in 5 mL ethanol. 50% aqueous hydrazine hydrate (41 μL, 0.64 mmol) was then added dropwise. The mixture was allowed to stir at room temperature for 48 hours. The solution was filtered, which afforded 0.2045 g (quantitative yield) of product as a white solid. .sup.1H-NMR (DMSO-d.sub.6, 400 MHz) δ 10.06 (bs, 1H), 9.29 (t, J=6.0 Hz, 1H), 7.52 (dd, J=1.4, 7.8 Hz, 1H), 7.31 (t, J=7.8 Hz, 1H), 7.17 (dd, J=1.4, 7.8 Hz, 1H), 4.52 (bs, 2H), 4.38 (d, J=6.4 Hz, 2H); .sup.13C-NMR (DMSO-d.sub.6, 100 MHz) δ 160.67, 158.24, 138.79, 132.21, 130.29, 129.59, 128.59, 127.48, 41.31; HRMS, DART calcd. for C.sub.9H.sub.10N.sub.3O.sub.2Cl.sub.2 [M+H.sup.+] 262.01500. found: 262.00989.

(285) The following compounds also were synthesized according to General Procedure F: C240, C241, C246, C248, C251, C255, C260, C261, C262, C263, C264, C266, C268, C278, C281, C282, C287, C289, C295, C296, C297, C301, C304, C305, C307, C310, C322, C336, C339, C340, C341, C362.

Example 6—Synthesis of Triazole Compounds of Formula XIII

(286) General Procedure G

(287) Compounds of formula XIII were synthesized according to the procedure described below and as shown in the following scheme:

(288) ##STR00258##

(289) An appropriately-substituted carboxylic acid (1 equiv.), an appropriately-substituted amidine (1.4 equiv.), HATU (1.1 equiv.), diisopropylethylamine (2.8 equiv.), and DMF were added to a round bottom flask, stirred at 25° C. and monitored via TLC (20% methanol in CH.sub.2Cl.sub.2 with 2 drops of acetic acid) for consumption of acid and formation of intermediate. Upon consumption of the acid, an appropriately-substituted hydrazine (1.5 equiv.) and acetic acid (13.8 equiv.) were added, and the mixture was stirred at 80° C. and monitored via TLC (20% methanol in CH.sub.2Cl.sub.2) for formation of product. The mixture was taken up in 4:1 ethyl acetate/hexane, washed with saturated NaHCO.sub.3 solution (3×), dried with MgSO.sub.4, and concentrated by rotary evaporation. Flash chromatography (10% methanol in DCM) was used to isolate compounds of formula XIII. The above procedure was adapted from that described in Castanedo, G. M. et al., J. Org. Chem. 2011, 76, 1177-1179, which is incorporated by reference in its entirety.

Synthesis of 4-(1-(tert-butyl)-3-phenyl-1H-1,2,4-triazol-5-yl)benzene-1,2-diol (compound C201)

(290) 4-(1-(Tert-butyl)-3-phenyl-1H-1,2,4-triazol-5-yl)benzene-1,2-diol (compound C201) was synthesized according to General Procedure G. 3,4-Dihydroxybenzoic acid (265 mg, 1.72 mmol), benzamidine.HCl (292 mg, 2.43 mmol), HATU (707 mg, 1.86 mmol), diisopropylethylamine (850 μL, 4.86 mmol), and DMF (6 mL) were added to a 25 mL round bottom flask, stirred at 25° C. and monitored via TLC (20% methanol in CH.sub.2Cl.sub.2 with 2 drops of acetic acid) for consumption of acid and formation of intermediate. Upon consumption of the acid, t-butylhydrazine.HCl (189 mg, 2.55 mmol) and acetic acid (1 mL, 23.8 mmol) were added, and the mixture was stirred at 80° C. and monitored via TLC (20% methanol in CH.sub.2Cl.sub.2) for formation of product. The mixture was taken up in 100 mL of 4:1 ethyl acetate/hexane, washed with saturated NaHCO.sub.3 solution (3×), dried with MgSO.sub.4, and concentrated by rotary evaporation. Flash chromatography (10% methanol in DCM) was used to isolate 14.6 mg of product (3%). .sup.1H NMR (CDCl.sub.3, 400 MHz) δ 8.10 (dd, J=1.36, 8.24 Hz, 2H), 7.5-7.36 (m, 3H), 6.77 (d, J=1.84 Hz, 1H), 6.72 (d, J=7.8 Hz, 1H), 6.66 (dd, J=1.84, 7.76 Hz, 1H), 1.51 (s, 9H); .sup.13C (CDCl.sub.3, 100 MHz) δ 157.86, 155.02, 147.25, 143.99, 130.51, 129.37, 128.82, 128.71, 126.35, 121.92, 117.84, 114.90, 61.83, 30.76.

(291) The following compounds also were synthesized according to General Procedure G: C208, C213, C214, C216, C220, C221, C222, C223.

Example 7—Synthesis of Thiazolidinedione Compounds of Formula XIV

3-(3-Bromobenzyl)thiazolidine-2,4-dione

(292) 3-(3-Bromobenzyl)thiazolidine-2,4-dione was synthesized according to the procedure described below and as shown in the following scheme:

(293) ##STR00259##

(294) To a solution of 2,4-thiazolidinedione (0.50 g, 4.27 mmol) and 3-bromobenzyl bromide (1.03 g, 4.12 mmol) in 12.5 mL absolute ethanol, 1.27 mL 5N NaOH was added and the reaction mixture was heated to reflux. After 26 h the reaction was diluted with 15 mL H.sub.2O and 20 mL ethyl acetate. The organic layer was washed with H.sub.2O (3×15 mL) and brine (3×15 mL), dried over sodium sulfate, filtered, and concentrated in vacuo. The resulting residue was purified by flash column chromatography on silica (20:80 ethyl acetate:hexane) to provide 0.254 g (21%) of product.

Synthesis of (Z)-3-(3-bromobenzyl)-5-(3,4-dihydroxybenzylidene)thiazolidine-2,4-dione (compound C206)

(295) (Z)-3-(3-bromobenzyl)-5-(3,4-dihydroxybenzylidene)thiazolidine-2,4-dione (compound C206) was synthesized according to the procedure described below and as shown in the following scheme:

(296) ##STR00260##

(297) A solution of 3-(3-bromobenzyl)thiazolidine-2,4-dione (0.123 g, 0.43 mmol), 3,4-dihydroxybenzaldehyde (0.060 g, 0.43 mmol), piperidine (6 drops), acetic acid (6 drops), and 8 mL toluene was heated at reflux for 40 min. The reaction mixture was concentrated and purified by column chromatography (40:60 ethyl acetate:hexane) to provide 0.039 g (22%) of product (compound C206).

(298) Compounds C199 and C203 were prepared according to above procedure except that 3-(benzyl)thiazolidine-2,4-dione and 3-(4-bromobenzyl)thiazolidine-2,4-dione, respectively, were used in place of 3-(3-bromobenzyl)thiazolidine-2,4-dione.

(Z)-5-(3,4-dihydroxybenzylidene)-3-((4′-(trifluoromethoxy)-[1,1′-biphenyl]-3-yl)methyl)thiazolidine-2,4-dione (compound C207)

(299) (Z)-5-(3,4-dihydroxybenzylidene)-3-((4′-(trifluoromethoxy)-[1,1′-biphenyl]-3-yl)methyl)thiazolidine-2,4-dione (compound C207) was synthesized according to the procedure described below and as shown in the following scheme:

(300) ##STR00261##

(301) To a mixture of compound C206 (0.0365 g, 0.090 mmol), 4-trifluoromethoxy phenylboronic acid (0.028 g, 0.131 mmol), 1.3 mL tetrahydrofuran, and 0.15 mL of 2M Na.sub.2CO.sub.3 was added tetrakis(triphenylphosphine) palladium (0.0029 g, 0.0025 mmol). The mixture was heated to reflux for 3 h. The reaction mixture was diluted with 20 mL H.sub.2O and acidified to pH 3 by dropwise addition of 1 N HCl. The solution was extracted with ethyl acetate (3×15 mL), and the combined organics were washed with H.sub.2O (3×15 mL), dried over MgSO.sub.4, filtered, and concentrated in vacuo. The resulted residue was recrystallized from ethanol to provide 0.034 g (78%) of product (compound C207).

(302) Additional synthetic methods for obtaining PAI-1 inhibitors are disclosed in EP 166469 A1, which is incorporated by reference in its entirety.

Example 8—Synthesis of Oxindole Compounds of Formula XV

Synthesis of 5-(2-chloroacetyl)indolin-2-one

(303) 5-(2-Chloroacetyl)indolin-2-one was synthesized according to the procedure described below and as shown in the following scheme:

(304) ##STR00262##

(305) To a 0° C. round bottom flask containing a suspension of 7.53 g AlCl.sub.3 (56.3 mmol) in 7 mL 1,2-dichloroethane, 3.6 mL (45.1 mmol) chloroacetyl chloride was added dropwise, resulting in a color change from yellow to dark red. The reaction was allowed to run for 1 hour after which a solution of 3.00 g oxindole (22.5 mmol) in 16 mL 1,2-dichloroethane was added. The reaction was allowed to run for an additional 2 hours at 0° C., and thereafter for another 3 hours at 45° C. The reaction was stopped by pouring into ice cold water, in which a tan precipitate formed immediately. The precipitate was filtered to provide 0.788 g (77%) of product as a beige solid.

Synthesis of 5,5′-(2,2′-(ethane-1,2-diylbis(azanediyl))bis(acetyl))bis(indolin-2-one) (compound C225)

(306) 5,5′-(2,2′-(Ethane-1,2-diylbis(azanediyl))bis(acetyl))bis(indolin-2-one) (compound C225) was synthesized according to the procedure described below and as shown in the following scheme:

(307) ##STR00263##

(308) To a round bottom flask, 7.2 mL of acetonitrile, 320 mg potassium iodide (1.93 mmol), 250 mg potassium carbonate (1.79 mmol), and 24 μL of 1,2-ethylenediamine were added. Thereafter, 150 mg of 5-(2-chloroacetyl)indolin-2-one was added to the reaction flask and the reaction was allowed to run overnight (under nitrogen, with stirring) at reflux at 85° C. The reaction flask contents were concentrated in vacuo and transferred into a separatory funnel using ethyl acetate. The product was then washed with a saturated salt solution (3×). The organic layer was dried, filtered, and concentrated in vacuo to provide 0.291 g (6.6%) of product (compound C225).

Example 9—Synthesis of Oxindole Compounds of Formula XVII

Synthesis of 5-(2-(3,4-dimethoxyphenyl)thiazol-4-yl)indolin-2-one (C227)

(309) 5-(2-(3,4-dimethoxyphenyl)thiazol-4-yl)indolin-2-one (compound C227) was synthesized according to the procedure described below and as shown in the following scheme:

(310) ##STR00264##

(311) To a round bottom flask containing a solution of 60 mg (0.286 mmol) 5-(2-chloroacetyl)indolin-2-one, 1.5 mL THF, and 1.5 mL absolute ethanol was added 57 mg (0.286 mmol) 3,4-dimethoxythiobenazmide. The reaction was stirred overnight under nitrogen at reflux (80° C.). The reaction flask contents were then concentrated in vacuo and the resulting residue was purified by trituration in acetone to provide 50 mg (50%) of product (compound C227).

(312) Compound C228 was prepared according to above procedure except that 3,4-dihydroxythiobenazmide was used in place of 3,4-dimethoxythiobenazmide.

Example 10—Synthesis of Oxindole Compounds of Formula XIX

Synthesis of 2-oxoindoline-5-sulfonyl chloride

(313) 2-Oxoindoline-5-sulfonyl chloride was synthesized according to the procedure described below and as shown in the following scheme:

(314) ##STR00265##

(315) To a round bottom flask, 2.00 g oxindole (15.0 mmol) was added in small portions to 4.0 mL (61.2 mmol) chlorosulfonic acid at 30° C. while stirring. After all of the oxindole was added, the temperature was reduced to 25° C. and was left to react for 1.5 hours under nitrogen gas. The reaction heated to 70° C. for an additional 1.5 hr. The reaction mixture was then placed in an ice bath and quenched by dropwise addition of deionized water to the reaction mixture. Upon addition of water, a pink precipitate was formed, which was isolated by suction filtration to provide 1.47 g (42%) of product as a dusty rose solid.

Synthesis of 2-oxo-N-phenethylindoline-5-sulfonamide (compound C229)

(316) 2-Oxo-N-phenethylindoline-5-sulfonamide (compound C229) was synthesized according to the procedure described below and as shown in the following scheme:

(317) ##STR00266##

(318) To a round bottom flask containing a solution of 100 mg 2-oxoindoline-5-sulfonyl chloride (0.432 mmol) in 9.5 mL methylene chloride, 0.119 mL (0.950 mmol) of phenethylamine was added. The reaction was stirred for 12 hours at room temperature under nitrogen gas. The reaction mixture was then diluted with 45 mL of ethyl acetate. This solution was washed with 1M HCl (3×15 mL) and a saturated salt solution (1×15 mL). The organic layer was collected and dried over magnesium sulfate, filtered, and concentrated in vacuo to provide 0.110 g (67%) of product (compound C229).

Example 11—Fluorometric PAI-1/uPA IC50 Plate Assay at pH 7.4

(319) To determine the efficacy of various synthesized compounds as PAI-1 inhibitors, a fluorometric plate assay was carried out to measure the half maximal inhibitory concentration (IC.sub.50) of these compounds on recombinant active human PAI-1 in vitro. An IC.sub.50 is a measure of the effectiveness of a compound in inhibiting biological or biochemical function. Stated another way, IC.sub.50 represents the concentration of a drug that is required for 50% inhibition in vitro. The IC.sub.50 of various compounds was measured using a fluorometric plate assay as set out below, and the results are shown in Tables 1 and 12.

(320) PAI-1 inhibitor compounds were dissolved in DMSO to a final concentration of (10-50 mM), depending upon solubility. Compounds were then diluted in physiologic buffer (40 mM HEPES, 100 mM NaCl, 0.05% Tween-20, pH7.4) containing 10% DMSO and a dilution series (from 0 to 1000 uM depending on solubility) was prepared. 80 μL of compound was added per well to a 96-well black, opaque microplate in duplicate. 10 μL of 20 nM Recombinant active human PAI-1 (Molecular Innovations) in physiologic buffer (40 mM HEPES, 100 mM NaCl, 0.05% Tween-20, 10% DMSO, pH 7.4), or physiologic buffer with 15 mg/mL, or physiologic buffer with 10% human plasma, was added and the mixture was agitated for 15 minutes at room temperature. 10 μL of 25 nM uPA (Rheotromb®) was added to each reaction well and the plate was agitated for an additional 30 minutes at room temperature. Tripeptide aminomethylcoumarin Gly-Gly-Arg-AMC (Calbiochem) fluorogenic substrate (100 μL of 100 μM) was then added and residual uPA activity was determined based upon cleavage of this substrate. The rate of AMC release by uPA (fluorescence) was measured at an excitation wavelength of 370 nm and an emission wavelength of 440 nm. Controls included PAI-1 and uPA in the absence of compound and uPA alone. Percent PAI-1 inhibition was calculated using the following formula: [(uPA alone-uPA/PAI-1+ compound)/(uPA alone-PAI-1/uPA)]*100%. The IC.sub.50 was calculated using Graphit (IC.sub.50 0-100%).

Example 12—Fluorometric PAI-1/uPA IC50 Plate Assay at pH 7.8

(321) To determine the efficacy of various synthesized compounds as PAI-1 inhibitors at pH 7.8, additional fluorometric plate assays were carried out to measure the half maximal inhibitory concentration (IC.sub.50) of these compounds on recombinant active human PAI-1 in vitro. The IC.sub.50 of each of these various compounds was measured using an assay as set out below, and the results are shown in Tables 3, 5, 7, 9, and 11.

(322) PAI-1 inhibitor compounds were dissolved in DMSO to a final concentration of (10-50 mM), depending upon solubility. Compounds were then diluted in physiologic buffer (40 mM HEPES, 100 mM NaCl, 0.05% Tween-20, pH7.8) containing (10% DMSO and a dilution series (from 0 to 1000 uM depending on solubility) was prepared. 80 μL of compound was added per well to a 96-well black, opaque microplate in duplicate. 10 μL of 20 nM PAI-1 in physiologic buffer (pH 7.8) was added and the mixture was agitated for 15 minutes at room temperature. 10 μL of 25 nM uPA was added and the plate was agitated for an additional 30 minutes at room temperature. 100 μL of 100 μM fluorogenic, tripeptide aminomethylcoumarin Gly-Gly-Arg-AMC (Calbiochem) fluorogenic substrate was then added and residual uPA activity was determined based upon cleavage of this substrate. Fluorescence, as a measure of the rate of AMC release by uPA, was read at the following wavelengths; excitation 370 nm, emission 440 nm. Controls included PAI-1 and uPA in the absence of compound and uPA alone. Percent PAI-1 inhibition was calculated using the following formula: [(uPA alone-uPA/PAI-1+ compound)/(uPA alone-PAI-1/uPA)]*100%. The IC.sub.50 was calculated using Graphit (IC.sub.50 0-100%).

Example 13—Fluorometric PAI-1/tPA IC50 Plate Assay at pH 7.8

(323) Additional fluorometric plate assays were carried out, as set out below, to determine the IC.sub.50 of various synthesized compounds as PAI-1 inhibitors. Results are shown in Tables 3, 5, 7, 9, and 11.

(324) PAI-1 inhibitor compounds were dissolved in DMSO to a final concentration of (10-50 mM), depending upon solubility. Compounds were then diluted in physiologic buffer (40 mM HEPES, 100 mM NaCl, 0.05% Tween-20, pH7.8) containing (10% DMSO and a dilution series (from 0 to 1000 μM depending on solubility) was prepared. 80 μL of compound was added per well to a 96-well black, opaque microplate in duplicate. 10 μL of 20 nM recombinant active human PAI-1 (Molecular Innovations) in physiologic buffer, as set out above, was added per well and the mixture was agitated for 15 minutes at room temperature. 10 uL of 25 nM human tissue type PA (tPA) (Activase® (alteplase), Genentech) was added per well and the plate was agitated for an additional 30 minutes at room temperature. Tissue type PA activity in each reaction mixture was determined by adding Phe-Gly-Arg-AMC fluorogenic substrate (100 μL of 100 μM) (Centerchem). The rate of AMC release by tPA was measured at an excitation wavelength of 370 nm and an emission wavelength of 440 nm. Controls included PAI-1 and tPA in the absence of compound and tPA alone. Percent PAI-1 inhibition was calculated using the following formula: [(tPA alone-tPA/PAI-1+ compound)/(tPA alone-PAI-1/tPA)]*100%. The IC50 is calculated using Graphit (IC50 0-100%).

Example 14—Fluorometric ATIII/αIIa IC50 Plate Assay at pH 7.8

(325) Additional fluorometric plate assays were carried out, as set out below, to determine the IC.sub.50 of various synthesized compounds as PAI-1 inhibitors. Results are shown in Tables 3, 5, and 7.

(326) Alpha-thrombin is an active enzyme, related to uPA or tPA. Alpha-thrombin is inhibited by the serpin ATIII, which is closely related to PAI-1. This assay was used therefore as a control in testing for specificity of the PAI-1 inhibitor compounds. Thus, any compound that is specific for inhibiting PAI-1 should not inhibit ATIII.

(327) PAI-1 inhibitor compounds were dissolved in DMSO to a final concentration of (10-50 mM), depending upon solubility. Compounds were then diluted in physiologic buffer (40 mM HEPES, 100 mM NaCl, 0.05% Tween-20, pH7.8) containing (10% DMSO and a dilution series (from 0 to 1000 μM depending on solubility) was prepared. 80 μL of compound was added per well to a 96-well black, opaque microplate in duplicate. 10 μL of 20 nM recombinant active anti-thrombin III (ATIII) (Molecular Innovations), a PAI-1-related protein, in physiologic buffer, as set out above, was added and the mixture was agitated for 15 minutes at room temperature. 10 μL of 25 nM human α-Thrombin (αIIa) (Haematologic Technologies) was added to each reaction well and the plate was agitated for an additional 30 minutes at room temperature. 100 μL of 100 μM fluorogenic, tripeptide aminomethylcoumarin benzoyl Phe-Val-Arg-AMC substrate was then added and residual αIIa activity was determined based upon cleavage of this substrate. The rate of AMC release by αIIa was measured at an excitation wavelength of 370 nm and an emission wavelength of 440 nm. Controls included PAI-1 and αIIa in the absence of compound and αIIa alone. Percent PAI-1 inhibition is calculated using the following formula: [(αIIa alone-αIIa/PAI-1+ compound)/(αIIa alone-PAI-1/αIIa)]*100%. The IC50 is calculated using Graphit (IC50 0-100%).

(328) The invention has been described in terms of particular embodiments found or proposed to comprise preferred modes for the practice of the invention. It will be appreciated by those of ordinary skill in the art that, in light of the disclosure, numerous modifications and changes can be made in the particular embodiments exemplified without departing from the intended scope of the invention. Therefore, it is intended that the appended claims cover all such equivalent variations which come within the scope of the invention as claimed.