Method and the device for diagnostics and therapy based on DNA resonance, a method for locating resonating DNA sequences in the genome, and a method for identifying the properties, structures, mechanisms, and frequencies of electromagnetic resonance in DNA molecules

Abstract

The present invention generally relates to a method and system of DNA resonance and its applications. In particular, the present invention provides a method and system of DNA resonance measurement which is done non-chemically. The invention is a device using an impedance spectroscopy and analyzing it to produce the diagnostic profile of the health and using initial treatments to see how the profile change and then adjusting the treatment to improve the profile in a specific direction, adjusting the parameters of the electric, electromagnetic and sound treatment for better outcomes. In this invention, the initial treatment is at 42.2 Gigahertz, which leads to Alu sequence decompaction and activation of the DNA leading to the improved biological outcomes, improved coherence and orderliness of the DNA and of the DNA fractal patterns. Further, the present invention is intended to various applications such as in therapy, communication to and from the body, communication to and from the brain, biotechnology and biological research using electromagnetic waves, patterned electric voltage, magnetic pulses, sound or other such means of stimulation applied individually and/or in combinations. Moreover, providing an algorithm of the conversion of DNA sequence into and from the wave patterns.

Claims

1. A method and system of DNA resonance and its applications, comprising of a device which is non-destructively affecting DNA and/or receiving information from DNA in live cells via non-chemical means characterized in that the device uses impedance spectroscopy and analyzing it to produce the diagnostic profile of an individual's health and using initial treatments to see how the profile change and then adjusting the treatment parameters field for improved outcome.

2. A method and system of DNA resonance and its applicationsas claimed in claim 1, wherein electromagnetic waves, electric voltage varied in time, magnetic pulses, sound or other such means of stimulation applied individually and/or in combinations is used in the device.

3. A method and system of DNA resonance and its applications as claimed in claim 1, whereinthe device used for initial treatment at 42.2 Gigahertz, which leads to Alusequence decompaction and activation of the DNA improved biological outcomes, improved the coherence and orderliness of the DNA and of the DNA fractal patterns.

4. A method and system of DNA resonance and its applicationsas claimed in claim 1, whereinthe device used for initial treatment at 42.2 Gigahertz, which leads to Aluand other repetitive elements sequence decompaction and activation of the DNA improved biological outcomes, improved the coherence and orderliness of the DNA and of the DNA fractal patterns.

5. A method and system of DNA resonance and its applications as claimed in claim 1, whereinthe main function of Alu and other repetitive elementsis vibrational and is responsible for the creation of the uniquely human (primate) morphogenic field and that is the key resonating component of our mind and consciousness.

6. A method and system of DNA resonance and its applications as claimed in claim 1, whereinAlus and other repetitive eiementsare creating the main part of the field in the nucleus and while interacting with the field they make the major contribution in the control of which genes are transcribed.

7. A method and system of DNA resonance and its applications as claimed in claim 1, whereinthe DNA sequence-specific activation and/or repression of certain DNA sequences are achieved.

8. A method and system of DNA resonance and its applications as claimed in claim 1, whereinthe device is used for application to therapy, communication to and from the body, communication to and from the brain, biotechnology and biological research.

9. A method and system of DNA resonance and its applications as claimed in claim 1, whereinon communication to and from the body, the microtubules (101) and DNA communicate resonantly through the nuclear membrane (102), and that the microtubules of neighboring cells communicate (104) resonantly through the contact points of the cell membranes (103), thereby integrating all the body nuclei by the waveguides.

10. A method and system of DNA resonance and its applications as claimed in claim 1, whereinresonant vibrations of DNA propagate not by chance, but are guided by waveguides of microtubules.

11. A method and system of DNA resonance and its applications as claimed in claim 1, whereinthe nervous system and connective tissue are primarily responsible for uniting the organism into one resonating system.

12. A method and system of DNA resonance and its applications as claimed in claim 1, whereinin neurons (203, 204), the propagation of action potential (201) across the axons is the process of reading and writing of information into and from microtubules (205) and that this propagation of action potential is electromagnetically connected (202) via the microtubules with the DNA in the nucleus thus allowing the participation of DNA in the work of mind and memory.

13. A method and system of DNA resonance and its applications as claimed in claim 1, whereinthe method and system where the sequential signals is patterned in time is claimed.

14. A method and system of DNA resonance and its applications as claimed in claim 1, whereinthe method and system where spatially patterned signals, the shape of the wave, which is its circular polarization, chirality and its 3-dimensional structure, is produced, is claimed.

15. A method and system of DNA resonance and its applications as claimed in claim 1, wherein the method and system of obtaining the patterns via bio-impedance spectroscopy, analyzing them, interpreting and returning to the body as DNA resonance treatment, in accordance with obtained information, is claimed.

16. A method and system of DNA resonance and its applications as claimed in claim 1, whereinelectromagnetic signature from DNA samples, or living tissues or living bodies is obtained and recording it, amplifying and returning back to activate or repress specific DNA sequence groups for therapy, biotechnology and other applications.

17. A method and system of DNA resonance and its applications as claimed in claim 1, whereinthe process of starting the treatment, then observing the changes via DNA resonance diagnostics and adjusting the treatment accordingly to improve the diagnostic results; which can be done is real time or in alternating steps during treatment and diagnostic, is claimed.

18. A method and system of DNA resonance and its applications as claimed in claim 1, whereinthe method where biofeedback is based on electric measurements, electromagnetic measurements, sound measurements, temperature measurements, optical measurements, optoacoustic or electroacoustic measurements, measurements of temperature, detailed pulse characteristics, bio-impedance spectroscopy, a response of the above measurements to active perturbation, and/or measurements performed at specific points including acupuncture points and Zakharyin-Ged Zones is claimed.

19. A method and system of DNA resonance and its applications as claimed in claim 1, whereinthe method locates purine-coded repetitive elements in DNA recoded into purine code (in which A and G are recoded to R; and C and T are recoded to Y) and target these-purine coded repetitive elements in physical therapy.

20. A method and system of DNA resonance and its applications as claimed in claim 1, whereinthe method locatesstrong-coded repetitive elements in DNA recoded into strong code (in which G and C are recoded to S; and A and T are recoded to W) and target these strong-coded repetitive elements in physical therapy.

21. A method and system of DNA resonance and its applications as claimed in claim 1, whereinthe method optimizes the field treatments by treating DNA samples or living biological objects with various treatments, measuring the effects on Alu and other repetitive elements and optimizing the treatment parameters to achieve optimal compaction or decompaction of Alu or other selected repetitive elements

22. A method and system of DNA resonance and its applications as claimed in claim 1, whereinthe method optimizes the field treatments by treating DNA samples or living biological objects with various treatments, measuring the effects on primary-coded, purine-coded and strong-coded repetitive elements and optimizing the treatment parameters to achieve optimal compaction or decompaction of selectedprimary-coded, purine-coded and strong-coded repetitive elements

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0033] A clear understanding of the key features of the invention summarized above may be had by reference to the appended drawings, which illustrate the method and system of the invention, although it will be understood that such figures depict preferred embodiments of the invention and, therefore, are not to be considered as limiting its scope with regard to other embodiments which the invention is capable of contemplating. Accordingly:

[0034] FIG. 1 illustrates [Spectrum] Frequency ranges used for therapy. (LLLT—low-level light therapy, PEMF—pulsed electromagnetic field).

[0035] FIG. 2 show [Table] the very approximate prediction of resonance wavelengths of genomic repeats.

[0036] FIG. 3 shows the recoding schemes used.

[0037] FIG. 4 shows the effect of sequence randomization on the HIDER counts.

[0038] FIG. 5 shows the enrichment of HIDER counts in the original sequences over randomized sequences. (*—P<0.05, **—P<0.01, ***—P<0.001, n.s.—nonsignificant)

[0039] FIG. 6 shows the length dependence of Purine HIDER enrichment in arabidopsis.

DETAILED DESCRIPTION OF THE INVENTION

[0040] Unless otherwise defined, all terms (including technical terms) used herein have the same meaning as commonly understood by one having an ordinary skill in the art to which the invention belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and the present disclosure and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

[0041] In describing the invention, it will be understood that a number of techniques are disclosed. Each of these has individual benefits and each can also be used in conjunction with one or more, or in some cases all, of the other disclosed techniques. Accordingly, for the sake of clarity, this description will refrain from repeating possible combination in an unnecessary fashion. Nevertheless, the specifications and claim/s should be read with the understanding that such combinations are entirely within the scope of the invention and the claim/s.

[0042] The present disclosure is to be considered as an exemplification of the invention and is not intended to limit the invention.

[0043] The invention is a method and system of DNA resonance and its applications.

[0044] In particular, this invention is a device which is non-destructively affecting DNA and/or receiving information from DNA in live cells via non-chemical means. It is also a device where one or more of the following is used: electromagnetic waves, electric voltage varied in time, magnetic pulses, sound or other such means of stimulation applied individually and/or in combinations.

[0045] According to a preferred embodiment of the present invention, the device uses impedance spectroscopy and analyzing it to produce the diagnostic profile of an individual's health and using initial treatments to see how the profile change and then adjusting the treatment parameters field for improved outcome.

[0046] According to a preferred embodiment of the present invention, the invention is a device used for initial treatment at 42.2 Gigahertz, which leads to Alu sequence decompaction and activation of the DNA improved biological outcomes, improved the coherence and orderliness of the DNA and of the DNA fractal patterns. Moreover, the inventors have suggested that the main function of Alu is vibrational and proposed that Alu is responsible for the creation of the uniquely human (primate) morphogenic field and that is the key resonating component of our mind and consciousness. Also, the inventors believe that Alus are creating the main part of the field in the nucleus and while interacting with the field they make the major contribution in the control of which genes are transcribed.

[0047] According to a preferred embodiment of the present invention, the DNA sequence-specific activation and/or repression of certain DNA sequences are achieved.

[0048] According to a preferred embodiment of the present invention, the device is used for application to therapy, communication to and from the body, communication to and from the brain, biotechnology and biological research. On communication to and from the body, referring to FIG. 1, it was proposed that the microtubules (101) and DNA communicate resonantly through the nuclear membrane (102), and that the microtubules of neighboring cells communicate (104) resonantly through the contact points of the cell membranes (103), thereby integrating all the body nuclei by the waveguides. From this, it follows that resonant vibrations of DNA propagate not by chance, but are guided by waveguides of microtubules. It was also proposed that the nervous system and connective tissue are primarily responsible for uniting the organism into one resonating system.

[0049] According to a preferred embodiment of the present invention, referring to FIG. 2, it was also proposed that in neurons (203, 204), the propagation of action potential (201) across the axons is the process of reading and writing of information into and from microtubules (205) and that this propagation of action potential is electromagnetically connected (202) via the microtubules with the DNA in the nucleus thus allowing the participation of DNA in the work of mind and memory.

[0050] According to a preferred embodiment of the present invention, another feature of this invention is the method and system where the sequential signals is patterned in time.

[0051] According to a preferred embodiment of the present invention, another embodiment of this invention is the method and system where spatially patterned signals are produced. Spatially patterned signals, the shape of the wave, which is its circular polarization, chirality and its 3-dimensional structure.

[0052] Furthermore, another embodiment of this invention is the method and system of obtaining the patterns via bio-impedance spectroscopy, analyzing them, interpreting and returning to the body as DNA resonance treatment, in accordance with obtained information.

[0053] Meanwhile, another embodiment of this invention is a method and system of obtaining an electromagnetic signature from DNA samples, or living tissues or living bodies and recording it, amplifying and returning back to activate or repress specific DNA sequence groups for therapy, biotechnology and other applications.

[0054] Moreover, another embodiment of this invention is the process of starting the treatment, then observing the changes via DNA resonance diagnostics and adjusting the treatment accordingly to improve the diagnostic results; which can be done is real time or in alternating steps during treatment and diagnostic.

[0055] Finally, another unique embodiment of this invention is the method where biofeedback is based on electric measurements, electromagnetic measurements, sound measurements, temperature measurements, optical measurements, optoacoustic or electroacoustic measurements, measurements of temperature, detailed pulse characteristics, bio-impedance spectroscopy, a response of the above measurements to active perturbation, and/or measurements performed at specific points including acupuncture points and Zakharyin-Ged Zones.

[0056] According to a preferred embodiment of the present invention, since Inventors believe that the majority of repetitive sequences in the genome are involved in meaningful, resonance signaling, Inventors hypothesized that some of the unique (non-repetitive) sequences in the genome might have evolved to resonate with the genomic repeats. Accordingly, Inventors hypothesized that it is not necessary for the unique sequence to be identical to the repeat, that for resonance, it might need to be only partially similar to the sequence of the repeat: for example, it is possible that some oscillations involve primarily the electron clouds of the aromatic rings (Savelyev et al., 2019). Therefore, only the purine-pyrimidine structure of the resonating sequences should be similar, while their primary sequences can be different. This simplification of the sequence from the primary sequence to the purine-pyrimidine sequence, is hereafter referred to as the “Purine code.” Similarly, for the oscillations primarily involving the hypothetical clouds of the delocalized protons of the hydrogen bonds in base pairs, only the patterns of these bonds should be similar, while the primary sequence can be different. This simplification of the sequence from primary to strong/weak (three bonds/two bonds per base pair), is hereafter referred to as the “Strong code.” The recoding rules used here are listed in FIG. 3.

[0057] Similarly, Amino and Thymine codes were used in the analysis. Therefore, Inventors attempted to search for sequences that are unique (non-repetitive), but become similar to genomic repeats or each other after recoding (simplification). Inventors will refer to them as HIDERs (Homologous If Decoded Elements, Repetitive). In accordance with the four recoding schemes, Table [Codes], four types of HIDERs were analyzed: Purine, Strong, Amine, and Thymine. On the primary sequence level, HIDERs are unique (non-repetitive) sequences, which are identical to each other after recoding. On the physical level, Inventors expect these to be engaged in resonance signaling, and therefore, enriched in the genomes of complex organisms. One of the advantages of such a computational genomics approach is that it is agnostic to the exact physical mechanism of the resonance, allowing verification of its existence prior to the discovery of the mechanism. Once HIDERs are found, their chemical structure may provide an insight into the modes of their resonance.

[0058] Methods

[0059] The repeats were masked using RepeatMasker (http://repeatmasker.org/) followed by a heuristic removal of repeats using Ugene 1.32.0 (http://ugene.net/). Recoding was done as described in Fig. [Codes]. HIDERs were detected by searching for similar pairs of fragments in the recoded sequences using Ugene, analyzed in Google Sheets, and plotted with GraphPad Prism. Randomized sequences were used as controls, see Supplement. To retain the distribution of nucleotide densities along the sequence, randomization was done only on the unmasked parts of the sequence within each 20-nucleotide bin. The significance of enrichment was determined using the t-test.

[0060] Results

[0061] HIDERs are enriched in genomes compared to randomized controls.

[0062] The inventors selected five species for analysis. In addition to humans, Inventors chose mice, drosophila, and arabidopsis as typical model species and the dolphin as a highly developed aquatic mammal.

[0063] In each genome, four 90 kb pieces were selected at random, and the repeats were masked. Randomized reference sequences (RAND) were created from the original sequences (ORIG). ORIG and RAND sequences were recoded, as presented in Table [Codes]. In each sequence, pairs of identical strings (HIDERs) longer than 19 bases were identified.

[0064] Among the five tested species, the highest enrichment of HIDERs was found in mammals and the lowest in drosophila. Among the recoding schemes, the highest enrichment was found in the Purine code and the lowest in the Thymine code. The highest statistical significance was observed for the enrichment of Purine HIDERs in humans and dolphins.

[0065] Length Dependence

[0066] In arabidopsis, the Purine HIDERs demonstrated a positive correlation of the HIDERs' enrichment with their length the enrichment was higher for longer HIDERs.

[0067] This suggests that longer HIDERs might be functional and, thus, preferentially selected during the process of evolution. Such correlation was less pronounced in the other species studied.

[0068] Discussion

[0069] As detailed in the Introduction, Inventors' initial motivation was to find sequence-dependent DNA resonators. Inventors realized that for resonance to be sustained, the number of DNA resonators needs to be very high in each cell, in the order of millions of copies. Logically, so-called “junk DNA” made of repetitive elements of various sizes, is the primary candidate for harboring DNA resonators. Inventors suggested that the key resonator in the human genome is the Alu element, which is represented by 1.1 million copies per cell. Then, Inventors proposed that since DNA resonators ought to serve a function in coordinating the operation of the cell and the transfer of information between cells, resonator sequences should evolve to be enriched in the genome. Moreover, inventors hypothesized that even non-repetitive (unique) sequences might resonate with the repetitive sequences if they support similar modes of oscillation, that is, similar frequencies and patterns of electromagnetic oscillations. Then, Inventors looked specifically for chemical structures in the DNA, which might support sequence-specific oscillations, and suggested that purine-pyrimidine patterns might be characterized by unique vibrational patterns. Specifically, Inventors hypothesized that the pi-electrons of aromatic rings of multiple nucleobases might form a collective delocalized electron cloud, shape and oscillation pattern, of which would be defined primarily by the purine-pyrimidine sequence. Therefore, DNA sequences, having a different primary sequence but common purine-pyrimidine patterns, might resonate. Inventors called such sequences HIDERs, and suggested that they might be enriched in the genome. Here, Inventors tested this hypothesis and confirmed the enrichment of the HIDERs in the selected mammal species and arabidopsis but not in drosophila, Fig. [Enrichment].

[0070] Similarly, Inventors hypothesized that protons of hydrogen bonds of neighboring base pairs would form a delocalized proton cloud (a proton highway). This cloud would be prone to oscillations, and these oscillations would depend on the DNA sequence, specifically on the order in which base pairs with two hydrogen bonds (weak: A, T) and three hydrogen bonds (strong: C, G), respectively, occur in the DNA sequence. As above, Inventors tested whether Strong HIDERs would be enriched in the genome. Inventors observed a significant enrichment in the dolphins, the mice, and arabidopsis, but not in humans or drosophila, Fig. [Enrichment].

[0071] Note that both the Purine and Strong codes are simplifying the sequence from fInventors' symbols (A, C, G, T) to two symbols (purine/pyrimidine or strong/weak). Some information, including side radicals of the nucleobases, is lost, presumably allowing the HIDERs of different primary sequences to resonate with each other and likely with high-copy genomic repeats. Although Inventors believe that high-copy genomic repeats are the primary resonators in the cell, Inventors focused here on HIDERs since they allow the inventors to test the DNA resonance hypothesis via computational genomics.

[0072] To reiterate, to Inventors' knowledge, this is the first, although indirect, evidence of DNA resonance in biology. However, although the obtained evidence is encouraging, more research is needed to verify the existence and the mechanistic details of DNA resonance. Computational modeling of the proposed electron and proton clouds of DNA sequences with the use of methods of quantum chemistry and structural biology could verify and substantiate the existence of such sequence-dependent resonating structures. Spectroscopic measurements could substantiate the proposed resonances between various sequences, including the ones highlighted by Inventors' analyses.

[0073] The inventors are aware that in addition to the DNA resonance explanation, there are possible explanations for the observed enrichments that could involve traditional chemical causes. For example, it is possible that purines are more likely to mutate into each other than into pyrimidines, and vice versa. Therefore, repetitive sequences via the process of mutation might diverge in their primary sequence while retaining their purine-pyrimidine sequence, thus, effectively becoming HIDERs. Similarly, certain repeats might be the targets of transcription factors, which recognize their strong-weak pair sequence while ignoring actual bases. Consequently, certain repeats might diverge in evolution, producing Strong HIDERs. Currently available evolutionary base-substitution rates are not precise enough to enable the delineation of the chemical and resonance causes for the enrichment signal of HIDERs. Therefore, Inventors hope that Inventors' results encourage further research and the hypothesis of sequence-dependent DNA resonance signaling will be verified more conclusively.

[0074] One of the challenges in using computational genomics as a tool for testing hypotheses is the need for the Bonferroni correction in the case of multiple comparisons. To avoid multiple comparisons, Inventors randomly selected the DNA fragments only once, and did not optimize any analysis parameters. The selection and analysis of the data occurred only once. Moreover, to allow for testing the phenomena observed by others, Inventors selected only a few species and only a small part (approximately 3%) of each genome. This way, others could easily reproduce the observed enrichments on untouched data sets.

[0075] Supplement

[0076] Sequences used for the analysis.

[0077] Four original and four randomized sequences were investigated,

[0078] Random sequence selection method: To avoid the multiple comparison problem in the statistics, the selection of sequences was performed only once. Each sequence was 90 Kb long. The selection was achieved using a simple algorithm, and the coordinates were predetermined using a simple rule. The assemblies and the coordinates were as follows:

[0079] Human

[0080] hg38_dna range=chr1: 100000000-100090000

[0081] hg38_dna range=chr1: 100090001-100180000

[0082] hg38_dna range=chr1: 100180001-100270000

[0083] hg38_dna range=chr1: 100270001-100360000

[0084] Dolphin

[0085] turTru2_dna range=JH472452:10000-100000

[0086] turTru2_dna range=JH472452:181000-271000

[0087] turTru2_dna range=JH472452:309250-399250

[0088] turTru2_dna range=JH472452:480250-570250

[0089] Mouse

[0090] mm10_dna range=chr3:32500000-32590000

[0091] mm10_dna range=chr3:32590001-32680000

[0092] mm10_dna range=chr3:32680001-32770000

[0093] mm10_dna range=chr3:32770001-32860000

[0094] Drosophila

[0095] dm6_dna range=chr2L:200000-290000

[0096] dm6_dna range=chr2L:400000-490000

[0097] dm6_dna range=chr2L:800000-890000

[0098] dm6_dna range=chr2L:1200000-1290000

[0099] Arabidopsis

[0100] hub_329263_araTha1_dna range=chr3:400000-490000

[0101] hub_329263_araTha1_dna range=chr3:600000-690000

[0102] hub_329263_araTha1_dna range=chr3:1490000-1580000

[0103] hub_329263_araTha1_dna range=chr3:1800000-1890000

[0104] Repeat Masking

[0105] Repeat masking was conducted in two steps. The original sequence was uploaded into the online RepeatMasker service (http://repeatmasker.org/cgi-bin/WEBRepeatMasker), and the repeats were masked with Ns. Then the sequence was masked by the Find Repeats algorithm of the UGENE program (Unipro UGENE http://ugene.net/).

[0106] Search for HIDERs in the Recoded Sequence.

[0107] The masked sequence was randomized as described in the Methods section. The original and randomized sequences were transformed into degenerate codes, as shown in Fig. [Codes]. Pairs of identical HIDERs longer than 19 pairs were identified using the Find Repeats algorithm of the UGENE program. The accuracy of the search was tested in part by verifying that the primary source sequences for the pairs of HIDERs were different as intended.

[0108] The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term includes any and all combinations of one or more of the associated listed items. As used herein, the singular forms are intended to include the plural forms as well as the singular forms, unless the context clearly indicates otherwise.

[0109] While the preferred embodiments of the present invention has been shown and described in detail, it is to be understood that numerous modifications can be made to the preferred embodiment without departing from the spirit of the invention. Therefore, it should be clearly understood that the forms of the present invention described above and shown in the figures of the accompanying drawings are illustrative only and are not intended to limit the scope of the present invention.

[0110] While the preferred embodiments of the present invention has been shown and described in detail, it is to be understood that numerous modifications can be made to the preferred embodiment without departing from the spirit of the invention. Therefore, it should be clearly understood that the forms of the present invention described above and shown in the figures of the accompanying drawings are illustrative only and are not intended to limit the scope of the present invention.