Cosmetic composition for whitening and improving the resilience of skin

Abstract

The present invention relates to a cosmetic composition for skin whitening and anti-aging, which contains 2 or more of collagen peptides, snake needle grass extract, and white ginseng saccharides as effective ingredients, improves skin resilience by increasing the content of collagen in the skin and suppresses the growth of melanin cells, improves skin brightness and uniformity, and alleviates skin yellowness and redness, so as to provide the effect of making the skin appear clearer and more radiant.

Claims

1. A method for whitening skin and increasing skin elasticity, comprising applying a cosmetic composition, which contains active ingredients consisting of an Oldenlandia diffusa extract, a collagen peptide, and a white ginseng polysaccharide, to the skin of a subject, wherein the collagen peptide is contained in a liquid crystal oil-in-water emulsion base, wherein the cosmetic composition is formulated into an oil-in-water emulsion, wherein each of the amounts of the Oldenlandia diffusa extract and the white ginseng polysaccharide is 0.001 to 10 wt %, respectively, based on the total weight of the composition, wherein the collagen peptide is contained in the amount of 0.001 to 0.05 wt % based on the total weight of the composition, and wherein the liquid crystal oil-in-water emulsion base is composed of two or more selected from the group consisting of sorbitan stearate, sorbitan laurate, behenyl alcohol, cetearyl alcohol, caprylic/capric triglyceride, cetearyl glucoside, polyglyceryl-10 pentastearate, and sodium stearoyl lactylate.

2. The method of claim 1, wherein the collagen peptide has a molecular weight of about 1,000 grams/mole.

3. The method of claim 1, wherein each of the amounts of the Oldenlandia diffusa extract and the white ginseng polysaccharide is 0.005-5 wt %, respectively, based on the total weight of the composition.

4. The method of claim 1, wherein the Oldenlandia diffusa extract is obtained by re-extracting with ethanol after extracting with boiling water, and the white ginseng polysaccharide extract is obtained by extracting with a mixed solvent of water and 1,3-butylene glycol.

Description

DESCRIPTION OF DRAWINGS

(1) FIG. 1 shows the results of evaluation (clinical test) of the skin brightness, uniformity, yellowness and redness on the human body using compositions of Example 3 and Comparative Example 1 for 7 weeks.

BEST MODE

(2) The cosmetic composition of the present invention contains, as an active ingredients, two or more of a collagen peptide, an Oldenlandia diffusa extract and a white ginseng polysaccharide.

(3) The collagen peptide that is used in the present invention may be contained in a liquid crystal oil-in-water emulsion base, and when the liquid crystal oil-in-water emulsion base containing the collagen peptide is used in combination with an Oldenlandia diffusa extract and a white ginseng polysaccharide, it can show synergistic effects on skin elasticity and whitening.

(4) The liquid crystal oil-in-water emulsion base that is used in the present invention may contain two or more selected from the group consisting of sorbitan stearate, sorbitan laurate, behenyl alcohol, cetearyl alcohol, caprylic/capric triglyceride, cetearyl glucoside, polyglyceryl-10 pentastearate, and sodium stearoyl lactylate.

(5) As used herein, the term “collagen” refers to a protein constituting connective tissue in the skin, tendons, bones and cartilages. It is a very important fibrous protein (albuminoid) placed between cells in the body of animals, including humans. Collagen is a polymer protein having a helix structure consisting of three polypeptide chains, each having a molecular weight of about 100,000. It improves the immune function of the human body, promotes the regeneration of cells to strengthen joints, and increases the metabolic activity and moisture-holding capacity of the skin to significantly improve skin beauty.

(6) Collagen peptides that are generally used are difficult to absorb transdermally, because they have a molecular weight ranging from tens of thousands to hundreds of thousands. However, the collagen peptide that is used in the present invention is a component having a molecular weight of about 1,000, prepared by hydrolyzing collagen having a molecular weight of about 100,000. It is easily absorbed into the skin due to its low molecular weight to reduce wrinkles and promote cell regeneration, and has excellent skin affinity and moisturizing ability.

(7) The collagen peptide that is used in the present invention can be prepared according to any conventional method known in the art, and the preparation method is not specifically limited. For example, the collagen peptide that is used in the present invention is prepared by a method comprising the steps of: (1) hydrolyzing collagen derived from the scales of marine fishes grown in a clean environment; (2) removing immunity-inducible components from the hydrolyzed collagen; and (3) reducing the molecular weight of the collagen of step (2).

(8) The collagen peptide of the present invention is contained in an amount of 0.001-0.05 wt % based on the total weight of the composition. If the content of the collagen peptide is less than 0.001 wt %, the skin beauty effect thereof will be insignificant, and if the content of the collagen peptide is more than 0.05 wt %, the stability thereof in the liquid crystal base will be poor.

(9) The Oldenlandia diffusa extract that is used in the present invention may be prepared by any method known in the art, and the preparation method is not specifically limited. For example, 1 kg of the dried whole plant of Oldenlandia diffusa is added to 10 liters of purified water, and then it is heated until boiling and is further heated for 10 minutes. After removing the water, the residue is washed, and then further washed by adding 10 liters of purified water thereto. Then, the residue is dried in air and added to 20 liters of 70% ethanol, after it is warmed and extracted under reflux in a reflux apparatus for 24 hours. After removing the solid using an 80-mesh sieve, the remaining filtrate is further filtered and concentrated, and the resulting solid is encapsulated in nanosomes. In this manner, the Oldenlandia diffusa extract can be obtained.

(10) The Oldenlandia diffusa extract prepared as described above is contained in an amount of 0.001-10 wt %, and preferably 0.005-5.0 wt %, based on the total weight of the composition. If the content of the Oldenlandia diffusa extract is less than 0.001 wt %, it cannot show a distinct effect, and if the content of the Oldenlandia diffusa extract is more than 10.0 wt %, the increase in the content will not lead to a distinct increase in the effect.

(11) The white ginseng polysaccharide that is used in the present invention may be prepared by any method known in the art, and the preparation method is not specifically limited. For example, dried white ginseng is added to purified water warmed to 60˜80° C., and it is washed for 10-20 minutes while being stirred at intervals. The washed white ginseng is added to a 7:3 (w/w) mixed solvent of purified water warmed to 80˜85° C.: 1,3-butylene glycol and is extracted for 6-8 hours while being stirred at intervals. The extract is cooled to 20˜30° C. and filtered to remove the solid. In this manner, the white ginseng polysaccharide extract can be obtained.

(12) The white ginseng polysaccharide prepared as described above is contained in an amount of 0.001-10 wt %, and preferably 0.005-5.0 wt %, based on the total weight of the composition. If the content of the white ginseng polysaccharide is less than 0.001 wt %, it cannot show a distinct effect, and if the content of the white ginseng polysaccharide is more than 10.0 wt %, the increase in the content will not lead to a distinct increase in the effect.

(13) The cosmetic composition of the present invention may further contain an oil component. The oil component may be natural oil or synthetic oil. Specifically, the oil component may be a hydrocarbon, triglyceride, ester or silicone oil.

(14) The oil component is contained in an amount of 1.0-40.0 wt %, and preferably 5.0-20.0 wt %, based on the total weight of the composition. If the content of the oil component is less than 1.0 wt %, it will be difficult to obtain a desired emulsion formulation, and if the content of the oil component is more than 40 wt %, the emulsion formulation will have poor stability.

(15) The cosmetic composition of the present invention may be formulated as an oil-in-water (O/W) or water-in-oil (W/O) emulsion composition. In addition, it may be formulated in the form of lotion, cream, emulsion, patch, stick or spray.

(16) The cosmetic composition of the present invention may further comprise, in addition to the above-described active ingredients, other components which are generally added to cosmetic compositions. Specific examples of these components include oils and fats, moisturizing agents, emollients, surfactants, organic and inorganic pigments, organic powder, UV absorbing agents, preservatives, disinfecting agents, antioxidants, plant extracts, pH adjusting agents, alcohols, dyes, fragrances, blood circulation stimulating agents, cooling agents, antiperspirants, purified water, and the like.

(17) The composition of the present invention can increase the collagen content of the skin to increase skin elasticity to thereby inhibit skin aging. In addition, it can inhibit the formation of melanocytes to alleviate skin pigmentations, including spots, freckles and discolorations, and alleviate the skin yellowness and redness to improve the skin brightness and uniformity.

MODE FOR INVENTION

(18) Hereinafter, the present invention will be described in further with reference to examples and test examples. It is to be understood, however, that these examples and test examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

TEST EXAMPLE 1

Effect on Inhibition of Melanin Production

(19) Human melanoma HM3KO cells (Y. Funasaka, Department of dermatology, Kobe university school of medicine, 5-1 Kusunoki-cho 7-chrome, Chuo-ku, Kobe 650, Japan) were added to 10% fetal bovine serum-containing minimum essential medium (MEM) and cultured under the conditions of 37° C. and 5% CO.sub.2. The cultured cells were plated on T75 flasks at a density of 3×10.sup.5 cells per flask and allowed to stand overnight until the cells adhered to the flask wall. After the cells have been confirmed to adhere to the flask wall, the medium was replaced with a fresh medium containing each of 10 ppm of an Oldenlandia diffusa extract, 10 ppm of a white ginseng polysaccharide and a mixture of 5 ppm of the Oldenlandia diffusa extract and 5 ppm of the white ginseng polysaccharide. In a control group, a DMSO-containing medium was used. While the medium was replaced with a fresh medium containing each test material at intervals of 2-3 days in this manner, the cells were cultured until the flask was filled with the cells. When the cells completely grew, the cells were collected, and the color thereof was compared between the control group and each of the groups treated with the test material. In addition, after the culture medium has been removed and the cells have been washed with PBS, the cells were dissolved in 1N sodium hydroxide, and the absorbance at 500 nm was measured. Based on the measurements, the percent inhibition of melanin production was calculated using the following equation 1, and the results of the calculation are shown in Table 1 below.
Percent inhibition of melanin production=100−(absorbance of each test material/absorbance of control×100)  Equation 1

(20) TABLE-US-00001 TABLE 1 Oldenlandia diffusa extract + Test Oldenlandia White white material diffusa ginseng ginseng (each 0.1%) Control extract extract extract Inhibition % 5 23 16 64

(21) As can be seen in Table 1 above, when the Oldenlandia diffusa extract and the white ginseng extract were used alone, melanin production was inhibited. In addition, when the Oldenlandia diffusa extract and the white ginseng extract were used in combination, the effect on the inhibition of melanin production was higher than when the Oldenlandia diffusa extract and the white ginseng extract were used alone. This suggests that the Oldenlandia diffusa extract and the white ginseng extract, which are used in the present invention, have excellent effects on the inhibition of melanin production.

TEST EXAMPLE 2

Measurement of Effect on Collagen Biosynthesis

(22) Human fibroblasts were cultured on a 24-well plate, and then the medium was replaced with a medium containing each test material at the concentration shown in Table 2 below, and the cells were cultured for 3 days. 0.5 ml of 10% fetal bovine serum-containing DMEM medium was added to each well, and then 10 μCi of L[2, 3, 4, 5-3H]-proline was added. After 24 hours, the medium and cells in each well were collected, washed with 5% TCA (trichloroacetic acid) solution, and dispensed into two test tubes. 1 Unit/μl of type I collagenase was added to one of the test tubes and incubated at 37° C. for 90 minutes, and the other test tube was stored at 4° C. Then, 0.05 ml of 50% trichloroacetic acid solution was added to each of the test tubes which were then allowed to stand at 4° C. for 20 minutes. Then, the content in each test tube was centrifuged at 12,000 rpm for 10 minutes, and the CPM (counts per minute) value of each supernatant and precipitate was measured using a liquid scintillation counter (LSC). Based on the measurements, the relative collagen biosynthesis value for each of the control group and the test groups was calculated using the following equation 2. In a control group, purified water was used in place of the test material. The results of the calculation were expressed relative to the control group (taken as 100) and are shown in Table 2 below.

(23) $\begin{array}{cc}\mathrm{RCB}\left(\%\right)=\frac{{\mathrm{collagen}}_{\mathrm{cpm}}}{\begin{array}{c}\left(\mathrm{total}{\mathrm{collagen}}_{\mathrm{cpm}}-{\mathrm{collagen}}_{\mathrm{cpm}}\right)\times \\ 5.4+{\mathrm{collagen}}_{\mathrm{cpm}}\end{array}}\times 100& \mathrm{Equation}2\end{array}$

(24) TABLE-US-00002 TABLE 2 Relative collagen biosynthesis (RCB) (%) Collagen liquid crystal Collagen Collagen base + Oldenlandia liquid crystal liquid crystal Oldenlandia Collagen diffusa + base + base + diffusa + Conc. liquid crystal Oldenlandia White ginseng White ginseng Oldenlandia White ginseng White ginseng (ppm) Control base diffusa polysaccharide polysaccharide diffusa polysaccharide polysaccharide 10 100 128 115 105 140 158 142 167 1 100 105 103 101 106 107 106 110

(25) As can be seen from the results in Table 2 above, the liquid crystal oil-in-water emulsion base containing the collagen peptide, the Oldenlandia diffusa extract and the white ginseng polysaccharide increased collagen biosynthesis in human skin cells in a concentration-dependent manner. The effect of promoting collagen biosynthesis was higher in the order of the liquid crystal oil-in-water emulsion base containing the collagen peptide >the Oldenlandia diffusa extract >the white ginseng polysaccharide. When two or more of these test materials were used in combination, a synergistic effect could be obtained, and when the liquid crystal oil-in-water emulsion base containing the collagen peptide, the Oldenlandia diffusa extract and the white ginseng polysaccharide were used in combination, the relative collagen biosynthesis (%) was the highest.

(26) Because it can be seen that the density of skin tissue increases as the content of collagen increases, a test for the collagen density of the dermal layer in the human skin was performed in the following manner.

REFERENCE EXAMPLE 1

Preparation of Compositions of Examples 1 to 3 and Comparative Examples 1 to 4

(27) Oil-in-water emulsion cosmetic compositions of Examples 1 to 3 and Comparative Examples 1 to 4, which have the compositions shown in Table 3 below, were prepared. The composition of Comparative Example 1 is a conventional oil-in-water emulsion cosmetic composition which does not contain the liquid crystal oil-in-water emulsion base containing the collagen peptide, the Oldenlandia diffusa extract and the white ginseng polysaccharide; the composition of Comparative Example 2 is a cosmetic composition which contains the liquid crystal oil-in-water emulsion base containing the collagen peptide, but does not contain the Oldenlandia diffusa extract and the white ginseng polysaccharide; and the cosmetic compositions of Comparative Examples 3 and 4 contain the Oldenlandia diffusa extract or the white ginseng polysaccharide. The cosmetic compositions of Examples 1 to 3 contain the liquid crystal oil-in-water emulsion base containing the collagen peptide, together with one or more of the Oldenlandia diffusa extract and the white ginseng polysaccharide. The liquid crystal oil-in-water emulsion base containing the collagen peptide was purchased from Chouette Co., Ltd. (Korea), and the Oldenlandia diffusa extract and the white ginseng polysaccharide were purchased from ACT Co., Ltd (Korea).

(28) TABLE-US-00003 TABLE 3 Comparative Comparative Comparative Comparative No. Raw material name Example 1 Example 2 Example 3 Example 4 Example 1 Example 2 Example 3 Example 4 1 Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 2 Disodium EDTA 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 3 Cetearyl alcohol 1 1 1 1 1 1 1 1 4 Stearic acid 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 5 Glyceryl stearate/PEG- 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 100 stearate 6 Polysorbate 60 1 1 1 1 1 1 1 1 7 Squalane 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 8 Preservative q.s. q.s. q.s. q.s. q.s. q.s. q.s. q.s. 9 Fragrance q.s. q.s. q.s. q.s. q.s. q.s. q.s. q.s. 10 Collagen liquid crystal 1 1 1 — — 1 — — base 11 Oldenlandia diffusa — 3 3 3 — — 3 — extract 12 White ginseng 3 — 3 3 — — — 3 polysaccharide

(29) Method for Preparation of Compositions of Examples 1 to 4 and Comparative Examples 1 to 4

(30) 1) Components 1 and 2 in Table 3 above were mixed uniformly and dissolved by heating to 70° C.

(31) 2) Components 3 to 9 in Table 3 above were mixed uniformly and dissolved by heating to 75° C.

(32) 3) The solution of step 2) was added slowly to the solution of step 1) with stirring (homomixing) at 70° C. to form an emulsion.

(33) 4) The emulsion of step 3) was cooled to 50° C. or below, and components 10 to 12 in Table 3 were added thereto and uniformly mixed, thereby preparing an oil-in-water emulsion cosmetic composition.

TEST EXAMPLE 3

Evaluation of Effect on Skin Elasticity

(34) Thirty five Korean women in their 30s to 50s were divided into 7 groups, each consisting of 5 persons. Each of the cosmetic compositions of Examples 1 to 3 and Comparative Examples 1 to 4 was applied to each group twice a day everyday for 6 weeks, the skin conditions were comparatively analyzed by measuring the dermal compactness using Dermascan C. The results of the analysis are shown in Table 4 below.

(35) TABLE-US-00004 TABLE 4 Percent increase Percent increase in elastic in dermal feeling/ Test material compactness questionnaire Example 1 30 49 Example 2 39 55 Example 3 46 59 Example 4 35 50 Comparative Example 1 6 3 Comparative Example 2 23 30 Comparative Example 3 19 23 Comparative Example 4 20 21

(36) As can be seen in Table 4, when the collagen peptide-containing liquid crystal oil-in-water emulsion base (Comparative Example 2), the Oldenlandia diffusa extract (Comparative Example 3) and the white ginseng polysaccharide (Comparative Example 4) were used alone, the collagen density of the dermis was increased. In addition, in the case of the composition of Example 3, which contains all the three test materials, the collagen density of the dermis significantly highly increased.

(37) These results indicate that the inventive cosmetic composition containing a combination of the collagen peptide-containing liquid crystal oil-in-water emulsion base, the Oldenlandia diffusa extract and the white ginseng polysaccharide has the effect of increasing the collagen density of the dermal layer. In addition, the results of questionnaire (sensory evaluation) indicate that the inventive composition actually increased the elastic feeling.

TEST EXAMPLE 4

Effect on Whitening of Human Skin

(38) In order to directly evaluate the skin whitening effects of the collagen peptide-containing liquid crystal oil-in-water emulsion base, the Oldenlandia diffusa extract and the white ginseng polysaccharide, thirty Korean women in their 30s to 50s (average age: 34.7) were divided into two groups, and each of the cosmetic compositions of Example 3 and Comparative Example 1 was applied to each group twice a day everyday for 7 weeks. The color of the skin was measured using a colorimeter (Minolta CR2002) in order to judge the effect of each composition on the skin conditions. Colors are indicated using the L*a*b* color system, in which the “L” value indicates brightness, the “b” value indicates yellowness, and the “a” value indicates redness. In addition, the uniformity of skin tone was measured using a spectrophotometer and comparatively analyzed. The results of the measurement are shown in FIG. 1.

(39) As can be seen in FIG. 1, when the composition of Example 3 according to the present invention was used, skin brightness and uniformity were increased by 1.12% and 6.35%, respectively, compared to when the composition of Comparative Example 1 was used. In addition, yellowness and redness were improved by 1.19% and 3.5%, respectively.