METHODS AND COMPOSITIONS FOR TREATING HEPATITIS B VIRUS INFECTIONS

Abstract

Described herein are methods for identifying compounds useful for the treatment of infection by hepatitis B virus (HBV) and for identifying compounds useful for the same.

Claims

1. A method for treating or clinically curing a patient infected by hepatitis B virus (HBV), the method comprising administering to the patient a therapeutically effective amount of a first compound capable of modulating core protein-mediated regulation of HBV DNA in an HBV infected cell of the patient.

2. The method of claim 1, wherein the therapeutically effective amount of the first compound is capable of modulating core protein assembly.

3. The method of claim 1, the method further comprising administering to the patient a therapeutically effective amount of a second compound capable of modulating core protein assembly.

4. The method of claim 3, wherein administration of the first and second compounds to the patient results in improved clinical outcome compared to administration of either compound alone.

5. The method of claim 4, wherein administration of the first and second compounds results in a synergistically improved clinical outcome compared to administration of either compound alone.

6. A method for treating or clinically curing a patient infected by hepatitis B virus (HBV), the method comprising administering to the patient a therapeutically effective amount of a compound capable of modulating core protein assembly, wherein the compound is administered at a dose sufficient to modulate core protein-mediated regulation of HBV DNA.

7. The method of claim 6, wherein administration of the compound alters levels of at least one of HBsAg, HBeAg, and viral RNA.

8. The method of claim 1 wherein the therapeutically effective amount of the first compound, when administered to the patient, is capable of one or more of the following: (a) modulating the structure of core protein; (b) modulating the function of core protein; (c) modulating the binding of core protein to HBV DNA; (d) depleting the amount of free core protein dimer available to bind to HBV DNA; (e) altering nuclear import or export of core protein; (f) altering an interaction between HBV DNA and a chromatin component; (g) altering an interaction between core protein and a chromatin component; (h) altering the rate, quantity, quality or stability of RNA expressed from HBV DNA; (i) altering the stability or maintenance of HBV DNA; and (j) modulating an innate immune response against HBV.

9. The method of claim 1, wherein the first compound acts allosterically or orthosterically.

10. The method of claim 1, further comprising administering at least one additional compound selected from the group consisting of: a nucleoside HBV polymerase inhibitor, a nucleotide HBV polymerase inhibitor, a modified nucleic acid, a peptide entry inhibitor, an interferon (Type I, II or III), a lymphotoxin beta agonist, a Toll-like receptor agonist, a non-nucleoside small molecule HBV polymerase inhibitor, a non-nucleotide small molecule HBV polymerase inhibitor, a compound capable of affecting capsid maturation, an HBV DNA transcriptional modulator, a DNA biosynthesis inhibitor, a subviral particle secretion inhibitor, a checkpoint modulator, an siRNA, a therapeutic vaccine, an entry inhibitor, a transcriptional modifier, a topoisomerase inhibitor, a compound that modulates presentation of HBV antigen via MHC, an HBV RNase H inhibitor, a proteasome inhibitor, a cyclophilin inhibitor, a transcription activator-like effector nuclease (TALEN), a DNA cleavage enzyme such as CAS9/CRISPR targeting HBV DNA, a dominant negative HBV mutant, a second mitochondrial-derived activator of caspases (SMAC) mimetic, a nucleic acid-based polymer (NAP) such as REP-2139Ca or REP-2055, a Stimulator of Interferon Genes (STING) such as DMXAA or 2′3′-cGAMP, and an inhibitory peptide.

11. The method of claim 10, wherein the checkpoint modulator is an anti-PD-1 antibody, an anti-PD-L1 antibody, or an anti-CTLA4 antibody.

12. The method of claim 10, wherein the siRNA targets HBV or host RNA.

13. The method of claim 1, wherein the first compound is: ##STR00077## ##STR00078## ##STR00079##

14. A method for identifying a compound useful for the treatment of infection by hepatitis B virus (HBV) and/or for clinically curing infection by HBV, comprising: (a) measuring the ability of the compound to modulate core protein-mediated regulation of HBV DNA; and (b) identifying the compound as useful for treating or clinically curing infection by HBV based on the ability of the compound to modulate core protein-mediated regulation of HBV DNA.

15. The method of claim 14, wherein measuring the ability of the compound to modulate core protein-mediated regulation of HBV DNA comprises measuring one or more of the following: (a) modulation of the structure of core protein by the compound; (b) modulation of the function of core protein by the compound; (c) modulation of the binding of core protein to HBV DNA by the compound; (d) depletion of the amount of free core protein dimer available to bind to HBV DNA by the compound; (e) alteration of nuclear import or export of core protein by the compound; (f) alteration of an interaction between HBV DNA and a chromatin component by the compound; (g) alteration of an interaction between core protein and a chromatin component by the compound; (h) alteration of the rate, quantity, quality or stability of RNA expressed from HBV DNA by the compound; (i) alteration of the stability or maintenance of HBV DNA by the compound; (j) modulation of an innate immune response against HBV by the compound; (k) alteration of the encapsidation of pgRNA by the compound; (l) recycling of HBV nucleic acid back to the nucleus to form HBV DNA; and (m) production of host proteins whose genes are modulated by core protein, including interferon stimulated genes.

16. The method of claim 14, wherein measuring the ability of the compound to modulate core protein-mediated regulation of HBV DNA comprises one or more of the following: (a) detecting a change in an amount of or state of core protein bound to HBV DNA, optionally by using a chromatin immunoprecipitation (ChIP) assay or immunoprecipitation and mass spectrometry; (b) performing a South-western blot of isolated HBV DNA; (c) evaluating isolated HBV DNA by a qPCR endpoint or real-time reporter assay; (d) measuring viral antigen by ELISA; (e) measuring viral RNA by qRT-PCR; (f) performing an endpoint or real-time reporter assay; (g) performing an assay using energy transfer or quenching between labeled core protein and HBV DNA or between another DNA binding protein and HBV DNA; (h) performing surface plasmon resonance (SPR); (i) performing biointerferometry; (j) performing a fluorescence-based method such as FRET, FP, or fluorescence quenching; and (k) detecting production of host proteins whose genes are modulated by core protein, including interferon stimulated genes.

17. The method of claim 14, wherein the ability of the compound to modulate core protein-mediated regulation of HBV DNA is measured by assessing binding of the compound to a core protein dimer to determine whether the compound affects binding interactions of HBV DNA to the core protein dimer.

18. The method of claim 14, wherein the ability of the compound to modulate core protein-mediated regulation of HBV DNA is measured using an assay comprising differentially reporter-tagged core protein subunits.

19. The method of claim 14, wherein the ability of the compound to modulate core protein-mediated regulation of HBV DNA is determined by measuring in vitro binding of core protein to HBV DNA, optionally comprising a competition assay with control DNA.

20. The method of claim 14, wherein the ability of the compound to modulate core protein-mediated regulation of HBV DNA is determined by measuring the presence or quantity of a viral protein or viral RNA.

21. The method of claim 20, wherein the method comprises measuring the presence or quantity of a HBsAg or HBeAg.

22. The method of claim 14, wherein the ability of the compound to modulate core protein-mediated regulation of HBV DNA is determined by an assay selected from the group consisting of: differential scanning fluorimetry, isothermal calorimetry, thermopheresis, and Saturation Transfer Difference NMR.

23. The method of claim 14, further comprising varying the concentration of the compound until the compound modulates core protein-HBV DNA interaction.

24. A method for identifying a compound useful for the treatment of infection by hepatitis B virus (HBV) and/or for clinically curing infection by HBV, comprising: (a) measuring the ability of the compound to modulate core protein assembly; and (b) identifying the compound as useful for treating and/or clinically curing infection by HBV based on the ability of the compound to modulate core protein.

25. The method of claim 24, further comprising measuring the ability of the compound to modulate core protein-mediated regulation of HBV DNA.

26. The method of claim 25, wherein the ability of the compound to modulate core protein-mediated regulation of HBV DNA is measured by assessing binding of a labeled compound to a core protein dimer to determine whether the compound affects binding interactions of HBV DNA with the core protein dimer.

27. The method of claim 24, wherein the ability of the compound to modulate core protein assembly is determined by measuring fluorescence quenching of labeled core protein.

28. The method of claim 24, wherein the ability of the compound to modulate core protein assembly is measured by an assay selected from the group consisting of: measuring altered binding of core protein to antibodies or other proteins sensitive to core protein tertiary or quaternary structure, immunoprecipitation and Western blot, sandwich ELISA, and a BRET assay.

29. The method of claim 1, wherein the HBV DNA is HBV cccDNA.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0098] FIG. 1 is a schematic showing that Cp is involved in multiple aspects of the HBV life cycle.

[0099] FIG. 2 shows that FL-HAP binds capsid with high affinity. FL-HAP was added to core protein dimer, which assembled. The mixture was resolved on a Superose 6 column. Absorbance monitored at 495 nm shows FL-HAP binding to and co-migrating with the capsid and large oligomer.

[0100] FIG. 3A-C shows a fluorescence quenching assay for depletion of free dimer. (A) Dimers have cysteines engineered at the C-termini, at either end, which is labeled with BoDIPY-FL. (B) Dimers are fluorescent (left) capsids (right) are not. (C) The change in fluorescence matches other methods of monitoring assembly, such as light scattering (LS), as shown.

[0101] FIG. 4 shows elution of Cp149 from a Superose 6 column, demonstrating how a CpAM-emulating Cp mutant can alter Stokes' radius.

DETAILED DESCRIPTION

Methods of Treatment

[0102] Described herein are methods for treating or clinically curing a patient infected by hepatitis B virus (HBV), including administering to the patient a therapeutically effective amount of a compound capable of modulating core protein-mediated regulation of DNA (e.g., cccDNA) in an HBV infected cell of the patient and/or modulating core protein assembly. In some embodiments, administration of the two compounds results in an improved clinical outcome compared to administration of either compound alone. In other embodiments, administration of the two compounds results in a synergistically improved clinical outcome compared to administration of either compound alone.

[0103] Also described herein are methods for treating or clinically curing a patient infected by hepatitis B virus (HBV), the method comprising the step of administering to the patient a compound capable of modulating core protein assembly, wherein the compound is administered at a dose sufficient to modulate core protein-mediated regulation of DNA (e.g., cccDNA). In some embodiments, administration of the compound alters HBV's S antigen (HBsAg), E antigen (HBeAg), or viral RNA levels. Methods for measuring HBsAg, HBeAg and RNA are well known in the art. For example methods for measuring HBsAg are described in Ly et al. (2006) J Clin Microbiol. 44(7): 2321-2326. Methods for measuring HBV DNA are found at www.accessdata.fda.gov/cdrh_docs/pdf8/P080026a.pdf. Methods for measuring HBeAg (and HBsAg) are described in Lee et al. (2011) Hepatology 53(5):1486-93. Methods for measuring viral RNA, e.g., pgRNA, are described in Lu et al. (2009) J Viral Hepat. 16(2):104-12; and Bai et al. (2013) Int J Hepatol. 2013:849290.

[0104] In certain embodiments the compound is capable of (a) modulating the structure of core protein; (b) modulating the function of core protein; (c) modulating the binding of core protein to DNA (e.g., cccDNA); (d) depleting the amount of free core protein dimer available to bind to cccDNA; (e) altering nuclear import or export of core protein; (f) altering an interaction between DNA (e.g., cccDNA) and a chromatin component; (g) altering an interaction between core protein and a chromatin component; (h) altering the rate, quantity, quality or stability of RNA expressed from DNA (e.g., cccDNA); (i) altering the stability or maintenance of DNA (e.g., cccDNA); and/or (j) modulating an innate immune response against HBV. In certain embodiments, the compound acts allosterically or orthosterically.

[0105] In some embodiments, the method also includes administering an additional compound. For example, one or more of the following compounds can be administered in combination with the compounds described herein: a nucleoside or nucleotide HBV polymerase inhibitor, a modified nucleic acid, a peptide entry inhibitor, an interferon (Type I, II or III), a lymphotoxin beta agonist, a Toll-like receptor agonist, a non-nucleoside small molecule HBV polymerase inhibitor, a non-nucleotide small molecule HBV polymerase inhibitor, a compound affecting capsid maturation, an HBV DNA (e.g., cccDNA) transcriptional modulator, a DNA (e.g., cccDNA) biosynthesis inhibitor, a subviral particle secretion inhibitor, a checkpoint modulator, an siRNA, a therapeutic vaccine, an entry inhibitor, a transcriptional modifier, a topoisomerase inhibitor, a compound that modulates presentation of HBV antigen via MHC, an HBV RNase H inhibitor, a proteasome inhibitor, a cyclophilin inhibitor, a transcription activator-like effector nuclease (TALEN), a DNA cleavage enzyme targeting HBV DNA (e.g., cccDNA), a dominant negative HBV mutant, a second mitochondrial-derived activator of caspases (SMAC) mimetic, nucleic acid-based polymer (NAP) such as REP-2139Ca and REP-2055, a Stimulator of Interferon Genes (STING) such as DMXAA and 2′3′-cGAMP, and an inhibitory peptide.

[0106] Nucleoside or nucleotide HBV polymerase inhibitors include, e.g., lamivudine (3TC, LMV), telbivudine, adefovir (ADV), entecavir (ETV), tenofovir, LB80380, lagociclovir valactate, pradefovir, emtricitabine, valtorcitabine, and amdoxovir. See, e.g., Hu et al. (2013) Annual Reports in Medicinal Chemistry 48:265-281. Modified nucleic acids include REP 9AC′. Id. Entry inhibitors include peptide entry inhibitors, e.g., Myrcludex-B. Id. See also, Interferons include type I, II or III interferons, e.g., Type III interferons (Interferon lambda). Id. Exemplary lymphotoxin beta agonists include those discussed in Lucifora et al. (2014) Science 343(6176):1221-8. Toll-like receptor agonists include, e.g., the Toll-like receptor-7 (TLR-7) agonist GS-9620. See, e.g., Hu et al. (2013) Annual Reports in Medicinal Chemistry 48:265-281. Non-nucleoside/nucleotide small molecule HBV polymerase inhibitors include foscarnet, oxymatrine, and compounds 10 and 11

##STR00015##

Id. Compounds affecting capsid maturation include, e.g., GLS-4 and HAP12. HBV cccDNA transcriptional modulators include, e.g., helioxanthin and derivatives 14 and 15

##STR00016##

quinolin-2-one and analog 17

##STR00017##

caudatin, and related compound 19

##STR00018##

See, e.g., Hu et al. (2013) Annual Reports in Medicinal Chemistry 48:265-281. cccDNA biosynthesis inhibitors include, e.g., sulfonamides CCC-0975 and CCC-0346. Id. Agents that block pgRNA encapsidation include isothiafludine (NZ-4), naphthylureas of the carbonyl J acid family (e.g., KM-1), and cIAP2. Subviral particle secretion inhibitors include HBF-0529, BM601 benzimidazole, and PBHBV-2-15. Id. Checkpoint modulators include an anti-PD-1 antibody, an anti-PD-L1 antibody, or an anti-CTLA4 antibody. siRNAs include siRNAs against HBV (e.g., ISIS-HBV mRNA-targeted antisense) or host RNA. See, also, e.g., Wooddell et al. (2013) Mol. Ther. 21(5):973-85. Therapeutic vaccines include GS-4774 (GlobeImmune) and those described in Obeng-Adjei et al. (2013) Cancer Gene Ther. 20(12):652-62. Transcriptional modifiers include Tenofovir and Entecavir. (See, e.g., Kitrinos et al. (2014) Hepatology 59(2):434-42. Topoisomerase inhibitors include, e.g., Topo II inhibitors, such as etoposide, and Topo I inhibitors, such as Irinitecan. Compounds that modulate presentation of HBV antigen via MHC include, e.g., chloroquine. (See, e.g., Accapezzato et al. (2005) J Exp Med. 202(6):817-28.) Methods for designing HBV RNase H inhibitors are discussed in Hayer et al. (2014) J Virol. 88(1):574-82. Proteasome inhibitors include, e.g., MG132. See, e.g., Wang et al. (2013) Hepatogastroenterology. 60(124):837-41. Cyclophilin inhibitors include, e.g., NVP-018 (Neurovive® Pharmaceutical AB), SCY-635, alisporivir, Sanglifehrin A derivatives, and OCB-030. Transcription activator-like effector nucleases (TALENs) targeting the HBV genome are described in, e.g., Bloom et al. (2013) Mol Ther. 21(10):1889-1897. DNA cleavage enzymes targeting HBV cccDNA are described in, e.g., Schiffer et al. (2013) DOI: 10.1371/journal.pcbi.1003131. Dominant negative HBV mutants are described in the art. Second mitochondrial-derived activator of caspases (SMAC) mimetics include birinapant (TL32711), LCL161 (Novartis), GDC-0917 (Genentech), HGS1029 (Human Genome Sciences), and AT-406 (Ascenta). Nucleic acid-based polymers (NAPs) include REP-2139Ca and REP-2055. Stimulator of Interferon Genes (STINGs) include DMXAA and 2′3′-cGAMP. Inhibitory peptides (e.g., NH.sub.2—SFYSVLFLWG TCGGFSHSWY-COOH; NH.sub.2-LCETVRFWPV CFCSLYVICS-COOH; NH.sub.2—SCAPAWSPAP TVVFVALYVV-COOH; NH.sub.2-QWGMDSLIRL YLWESLGLLS-COOH; NH.sub.2-IHPLSRGNFF PHVRLMGEWR-COOH; NH.sub.2-GQALCAGVSL FADWLHESTL-COOH; NH.sub.2-LKHFDPRWPL MSLMSSWACM-COOH; NH.sub.2-PPLRKAFCWR CFNWLSTKRL-COOH; and NH.sub.2-LRKSMLKVGR DVCYVSLWVF-COOH) are described in International Patent Publication WO2000/042063.

Compounds

[0107] The methods described herein can include administration of any one of the following compounds:

##STR00019## ##STR00020## ##STR00021##

[0108] The methods described herein can include administration of any of the capsid promoting and HBsAg reducing molecules described in International Publication No. WO/2015/057945, U.S. Provisional Patent Application No. 62/148,994, and in International Patent Application No. PCT/US2015/020444.

[0109] For example, the methods described herein can include administration of a compound of Formula 1 (from International Publication No. WO/2015/057945) having the structure:

##STR00022##

or a pharmaceutically acceptable salt or stereoisomer thereof, wherein: [0110] X is selected from the group consisting of

##STR00023## [0111] q is 0, 1, 2, 3 or 4; [0112] p is 0, 1, 2, 3, or 4; [0113] r is 0, 1, 2, 3, or 4; [0114] R.sup.1 is independently for each occurrence selected from the group consisting of —H, —C.sub.1-C.sub.6alkyl, —C.sub.1-C.sub.6alkoxy, —C.sub.1-C.sub.6alkyl-O—C.sub.1-C.sub.6alkyl, halogen, cyano, —OH, —C(O)H, —CO.sub.2R′, —C(O)N(R′)(R″), —C(O)C.sub.1-C.sub.6alkyl, —N(R′)(R″), —NO.sub.2, —N(R)C(O)C.sub.1-C.sub.6alkyl, —S(O).sub.w—C.sub.1-C.sub.6alkyl, —N(R)S(O).sub.w—C.sub.1-C.sub.6alkyl, and —S(O).sub.w—N(R′)(R″); [0115] w is 0, 1 or 2; [0116] R′ is independently for each occurrence selected from the group consisting of —H and —C.sub.1-C.sub.6alkyl; [0117] R″ is independently for each occurrence selected from the group consisting of —H and —C.sub.1-C.sub.6alkyl; or R′ and R″ are taken together with the nitrogen atom to which they are attached to form a 4-7 membered heterocyclic ring; [0118] R.sup.2 is independently for each occurrence selected from the group consisting of —H, —C.sub.1-C.sub.6alkyl, —C.sub.1-C.sub.6alkoxy, —C.sub.1-C.sub.6alkyl-O—C.sub.1-C.sub.6alkyl, halogen, cyano, —OH, —C(O)H, —CO.sub.2R′, —C(O)N(R′)(R″), —C(O)C.sub.1-C.sub.6alkyl, —N(R′)(R″), —NO.sub.2, —N(R)C(O)C.sub.1-C.sub.6alkyl, —S(O).sub.w—C.sub.1-C.sub.6alkyl, —N(R)S(O).sub.w—C.sub.1-C.sub.6alkyl, and —S(O).sub.w—N(R′)(R″); and [0119] R.sup.3 is selected from the group consisting of —H and —C.sub.1-C.sub.6alkyl;

[0120] wherein C.sub.1-C.sub.6alkyl or C.sub.1-C.sub.6alkoxy may be independently for each occurrence optionally substituted with one, two, or three halogens.

[0121] The methods described herein can also include a compound of Formula 1 (described in U.S. Provisional Patent Application No. 62/148,994) having the structure:

##STR00024##

or a pharmaceutically acceptable salt or stereoisomer thereof, wherein:

##STR00025##

is selected from the group consisting of phenyl, naphthyl, and heteroaryl; [0122] Y is selected from the group consisting of a bond, —O—, —S(O).sub.w—, and —N(R′)—; [0123] X is selected from the group consisting of phenyl, naphthyl, and heteroaryl; wherein X [0124] is optionally substituted with one, two, three, or four R.sup.2 groups; [0125] provided that at least one of

##STR00026##

or X is a heteroaryl; [0126] R.sup.1 is independently for each occurrence selected from the group consisting of —H, —C.sub.1-C.sub.6alkyl, —C.sub.1-C.sub.6alkoxy, —C.sub.1-C.sub.6alkyl-O—C.sub.1-C.sub.6alkyl, halogen, cyano, —OH, —C(O)H, —CO.sub.2R′, —C(O)N(R′)(R″), —C(O)C.sub.1-C.sub.6alkyl, —N(R′)(R″), —NO.sub.2, —N(R)C(O)C.sub.1-C.sub.6alkyl, —S(O).sub.w—C.sub.1-C.sub.6alkyl, —N(R)S(O).sub.w—C.sub.1-C.sub.6alkyl, and —S(O).sub.w—N(R′)(R″); [0127] q is 0, 1, 2, 3 or 4; [0128] w is 0, 1 or 2; [0129] R′ is independently for each occurrence selected from the group consisting of —H and —C.sub.1-C.sub.6alkyl; [0130] R″ is independently for each occurrence selected from the group consisting of —H and —C.sub.1-C.sub.6alkyl; or R′ and R″ are taken together with the nitrogen atom to which they are attached to form a 4-7 membered heterocyclic or heteroaryl ring, each of which is optionally substituted with an oxo group; [0131] R.sup.2 is independently for each occurrence selected from the group consisting of —H, —C.sub.1-C.sub.6alkyl, —C.sub.1-C.sub.6alkoxy, —C.sub.1-C.sub.6alkyl-O—C.sub.1-C.sub.6alkyl, halogen, oxo, cyano, —OH, —C(O)H, —CO.sub.2R′, —C(O)N(R′)(R″), —C(O)C.sub.1-C.sub.6alkyl, —N(R′)(R″), —NO.sub.2, —N(R)C(O)C.sub.1-C.sub.6alkyl, —S(O).sub.w—C.sub.1-C.sub.6alkyl, —N(R)S(O).sub.w—C.sub.1-C.sub.6alkyl, and —S(O).sub.w—N(R′)(R″); and [0132] R.sup.3 is selected from the group consisting of —H, —C.sub.1-C.sub.6alkyl, —N(R′)(R″), —N(R′)C.sub.1-C.sub.6alkyl-N(R′)(R″), —N(R′)—C.sub.1-C.sub.6alkyl-OR′, —OH, —C.sub.1-C.sub.6alkoxy, —O—C.sub.1-C.sub.6alkyl-OR′, —O-heterocyclyl, —O-heteroaryl, —O—C.sub.1-C.sub.6alkyl-heteroaryl, —C.sub.1-C.sub.6alkyl-heteroaryl, heterocyclyl, and heteroaryl, wherein heterocyclyl and heteroaryl are optionally substituted with one or two C.sub.1-C.sub.6alkyl or halogen; [0133] wherein C.sub.1-C.sub.6alkyl or C.sub.1-C.sub.6alkoxy may be independently for each occurrence optionally substituted with one, two, or three halogens.

[0134] The methods described herein can also include a compound of Formula 2 (described in U.S. Provisional Patent Application No. 62/148,994) having the structure:

##STR00027##

or a pharmaceutically acceptable salt or stereoisomer thereof, wherein:

##STR00028##

is selected from the group consisting of phenyl, naphthyl, and heteroaryl; [0135] Y is selected from the group consisting of a bond, —O—, and —S(O).sub.w—; [0136] X is selected from the group consisting of phenyl, naphthyl, and heteroaryl; wherein X is optionally substituted with one, two, three, or four R.sup.2 groups; [0137] R.sup.1 is independently for each occurrence selected from the group consisting of —H, —C.sub.1-C.sub.6alkyl, —C.sub.1-C.sub.6alkoxy, —C.sub.1-C.sub.6alkyl-O—C.sub.1-C.sub.6alkyl, halogen, cyano, —OH, —C(O)H, —CO.sub.2R′, —C(O)N(R′)(R″), —C(O)C.sub.1-C.sub.6alkyl, —N(R′)(R″), —NO.sub.2, —N(R)C(O)C.sub.1-C.sub.6alkyl, —S(O).sub.w—C.sub.1-C.sub.6alkyl, —N(R)S(O).sub.w—C.sub.1-C.sub.6alkyl, and —S(O).sub.w—N(R′)(R″); [0138] q is 0, 1, 2, 3 or 4; [0139] w is 0, 1 or 2; [0140] R′ is independently for each occurrence selected from the group consisting of —H and —C.sub.1-C.sub.6alkyl; [0141] R″ is independently for each occurrence selected from the group consisting of —H and —C.sub.1-C.sub.6alkyl; or R′ and R″ are taken together with the nitrogen atom to which they are attached to form a 4-7 membered heterocyclic or heteroaryl ring, each of which is optionally substituted with an oxo group; [0142] R.sup.2 is independently for each occurrence selected from the group consisting of —H, —C.sub.1-C.sub.6alkyl, —C.sub.1-C.sub.6alkoxy, —C.sub.1-C.sub.6alkyl-O—C.sub.1-C.sub.6alkyl, halogen, oxo, cyano, —OH, —C(O)H, —CO.sub.2R′, —C(O)N(R′)(R″), —C(O)C.sub.1-C.sub.6alkyl, —N(R′)(R″), —NO.sub.2, —N(R)C(O)C.sub.1-C.sub.6alkyl, —S(O).sub.w—C.sub.1-C.sub.6alkyl, —N(R)S(O).sub.w—C.sub.1-C.sub.6alkyl, and —S(O).sub.w—N(R′)(R″); and [0143] R.sup.3 is selected from the group consisting of —H, —C.sub.1-C.sub.6alkyl, —N(R′)(R″), —N(R′)C.sub.1-C.sub.6alkyl-N(R′)(R″), —N(R′)—C.sub.1-C.sub.6alkyl-OR′, —OH, —C.sub.1-C.sub.6alkoxy, —O—C.sub.1-C.sub.6alkyl-OR′, —O-heterocyclyl, —O-heteroaryl, —O—C.sub.1-C.sub.6alkyl-heteroaryl, —C.sub.1-C.sub.6alkyl-heteroaryl, heterocyclyl, and heteroaryl, wherein heterocyclyl and heteroaryl are optionally substituted with one or two C.sub.1-C.sub.6alkyl or halogen; [0144] wherein C.sub.1-C.sub.6alkyl or C.sub.1-C.sub.6alkoxy may be independently for each occurrence optionally substituted with one, two, or three halogens.

[0145] The methods described herein can also include a compound of Formula 3 (described in U.S. Provisional Patent Application No. 62/148,994) having the structure:

##STR00029##

or a pharmaceutically acceptable salt or stereoisomer thereof, wherein:

##STR00030##

is selected from the group consisting of phenyl, naphthyl, and heteroaryl; [0146] Y is selected from the group consisting of a bond, —O—, —S(O).sub.w—, and —N(R′)—; [0147] Z is CH or N; [0148] X is selected from the group consisting of phenyl, naphthyl, and heteroaryl; wherein X is optionally substituted with one, two, three, or four R.sup.2 groups; [0149] R.sup.1 is independently for each occurrence selected from the group consisting of —H, —C.sub.1-C.sub.6alkyl, —C.sub.1-C.sub.6alkoxy, —C.sub.1-C.sub.6alkyl-O—C.sub.1-C.sub.6alkyl, halogen, cyano, —OH, —C(O)H, —CO.sub.2R′, —C(O)N(R′)(R″), —C(O)C.sub.1-C.sub.6alkyl, —N(R′)(R″), —NO.sub.2, —N(R)C(O)C.sub.1-C.sub.6alkyl, —S(O).sub.w—C.sub.1-C.sub.6alkyl, —N(R)S(O).sub.w—C.sub.1-C.sub.6alkyl, and —S(O).sub.w—N(R′)(R″); [0150] q is 0, 1, 2, 3 or 4; [0151] w is 0, 1 or 2; [0152] R′ is independently for each occurrence selected from the group consisting of —H and —C.sub.1-C.sub.6alkyl; [0153] R″ is independently for each occurrence selected from the group consisting of —H and —C.sub.1-C.sub.6alkyl; or R′ and R″ are taken together with the nitrogen atom to which they are attached to form a 4-7 membered heterocyclic or heteroaryl ring, each of which is optionally substituted with an oxo group; [0154] R.sup.2 is independently for each occurrence selected from the group consisting of —H, —C.sub.1-C.sub.6alkyl, —C.sub.1-C.sub.6alkoxy, —C.sub.1-C.sub.6alkyl-O—C.sub.1-C.sub.6alkyl, halogen, oxo, cyano, —OH, —C(O)H, —CO.sub.2R′, —C(O)N(R′)(R″), —C(O)C.sub.1-C.sub.6alkyl, —N(R′)(R″), —NO.sub.2, —N(R)C(O)C.sub.1-C.sub.6alkyl, —S(O).sub.w—C.sub.1-C.sub.6alkyl, —N(R)S(O).sub.w—C.sub.1-C.sub.6alkyl, and —S(O).sub.w—N(R′)(R″); and [0155] R.sup.3 is selected from the group consisting of —H, —C.sub.1-C.sub.6alkyl, —N(R′)(R″), —N(R′)C.sub.1-C.sub.6alkyl-N(R′)(R″), —N(R′)—C.sub.1-C.sub.6alkyl-OR′, —OH, —C.sub.1-C.sub.6alkoxy, —O—C.sub.1-C.sub.6alkyl-OR′, —O-heterocyclyl, —O-heteroaryl, —O—C.sub.1-C.sub.6alkyl-heteroaryl, —C.sub.1-C.sub.6alkyl-heteroaryl, heterocyclyl, and heteroaryl, wherein heterocyclyl and heteroaryl are optionally substituted with one or two C.sub.1-C.sub.6alkyl or halogen; [0156] wherein C.sub.1-C.sub.6alkyl or C.sub.1-C.sub.6alkoxy may be independently for each occurrence optionally substituted with one, two, or three halogens.

[0157] The methods described herein can also include a compound of Formula 4 (described in U.S. Provisional Patent Application No. 62/148,994) having the structure:

##STR00031##

or a pharmaceutically acceptable salt or stereoisomer thereof, wherein:

##STR00032##

is selected from the group consisting of phenyl, naphthyl, and heteroaryl; [0158] Y is selected from the group consisting of a bond, —O—, —S(O).sub.w—, and —N(R′)—; [0159] X is selected from the group consisting of phenyl, naphthyl, and heteroaryl; wherein X is optionally substituted with one, two, three, or four R.sup.2 groups; [0160] R.sup.1 is independently for each occurrence selected from the group consisting of —H, —C.sub.1-C.sub.6alkyl, —C.sub.1-C.sub.6alkoxy, —C.sub.1-C.sub.6alkyl-O—C.sub.1-C.sub.6alkyl, halogen, cyano, —OH, —C(O)H, —CO.sub.2R′, —C(O)N(R′)(R″), —C(O)C.sub.1-C.sub.6alkyl, —N(R′)(R″), —NO.sub.2, —N(R)C(O)C.sub.1-C.sub.6alkyl, —S(O).sub.w—C.sub.1-C.sub.6alkyl, —N(R)S(O).sub.w—C.sub.1-C.sub.6alkyl, and —S(O).sub.w—N(R′)(R″); [0161] q is 0, 1, 2, 3 or 4; [0162] w is 0, 1 or 2; [0163] R′ is independently for each occurrence selected from the group consisting of —H and —C.sub.1-C.sub.6alkyl; [0164] R″ is independently for each occurrence selected from the group consisting of —H and —C.sub.1-C.sub.6alkyl; or R′ and R″ are taken together with the nitrogen atom to which they are attached to form a 4-7 membered heterocyclic or heteroaryl ring, each of which is optionally substituted with an oxo group; [0165] R.sup.2 is independently for each occurrence selected from the group consisting of —H, —C.sub.1-C.sub.6alkyl, —C.sub.1-C.sub.6alkoxy, —C.sub.1-C.sub.6alkyl-O—C.sub.1-C.sub.6alkyl, halogen, oxo, cyano, —OH, —C(O)H, —CO.sub.2R′, —C(O)N(R′)(R″), —C(O)C.sub.1-C.sub.6alkyl, —N(R′)(R″), —NO.sub.2, —N(R)C(O)C.sub.1-C.sub.6alkyl, —S(O).sub.w—C.sub.1-C.sub.6alkyl, —N(R)S(O).sub.w—C.sub.1-C.sub.6alkyl, and —S(O).sub.w—N(R′)(R″); and [0166] R.sup.3 is selected from the group consisting of —N(R′)(R″), —N(R)C.sub.1-C.sub.6alkyl-N(R′)(R″), —N(R)—C.sub.1-C.sub.6alkyl-OR′, —OH, —C.sub.1-C.sub.6alkoxy, —O—C.sub.1-C.sub.6alkyl-OR′, —O-heterocyclyl, —O-heteroaryl, —O—C.sub.1-C.sub.6alkyl-heteroaryl, —C.sub.1-C.sub.6alkyl-heteroaryl, heterocyclyl, and heteroaryl, wherein heterocyclyl and heteroaryl are optionally substituted with one or two C.sub.1-C.sub.6alkyl or halogen; [0167] wherein C.sub.1-C.sub.6alkyl or C.sub.1-C.sub.6alkoxy may be independently for each occurrence optionally substituted with one, two, or three halogens

[0168] The methods described herein can also include a compound of Formula 4 (described in International Patent Application No. PCT/US2015/020444) having the structure:

##STR00033##

wherein:

[0169] T is selected from the group consisting of —C(O)—, —CH.sub.2—C(O)—, —N(C(O)—CH.sub.3)—, —NH—, —O—, and S(O).sub.z—, where z is 0, 1, or 2;

[0170] Y is C(R.sup.11).sub.2, S(O).sub.y, NR.sub.Y and O wherein y is 0, 1, or 2;

[0171] R.sub.Y is selected from the group consisting of H, methyl, ethyl, propyl, phenyl and benzyl;

[0172] R.sub.L is selected from the group consisting of H, methyl, and —C(O)—C.sub.1-3alkyl;

[0173] L is a bond or C.sub.1-4 straight chain alkylene optionally substituted by one or two substituents each independently selected from the group consisting of methyl (optionally substituted by halogen or hydroxyl), ethenyl, hydroxyl, NR′R″, phenyl, heterocycle, and halogen and wherein the C.sub.1-4 straight chain alkylene may be interrupted by an —O—;

[0174] R.sup.2 is selected from the group consisting of H, phenyl or naphthyl (wherein the phenyl or naphthyl may be optionally substituted with one, two, three or more substituents selected from the group consisting of halogen, hydroxyl, nitro, cyano, carboxy, C.sub.1-6alkyl, C.sub.2-6alkenyl, C.sub.2-6alkynyl, C.sub.1-6alkoxy, NR′R″, —C(O)—NR′R″, —C(O)—C.sub.1-6alkyl, —C(O)—C.sub.1-6alkoxy, phenyl (optionally substituted by one, two or three substituents each independently selected from the group consisting of halogen, hydroxyl, cyano, C.sub.1-6alkyl, C.sub.2-6alkenyl, C.sub.2-6alkynyl, C.sub.1-6alkoxy, NR′R″, —C(O)—NR′R″, —C(O)—C.sub.1-6alkyl, —C(O)—C.sub.1-6alkoxy, —S(O).sub.w—C.sub.1-6alkyl (where w is 1, 2, or 3), S(O).sub.w—NR′R″ (where w is 1, 2 or 3), —NR′—S(O).sub.w, and —S(O).sub.w—NR′R″ (where w is 1, 2, or 3)), heteroaryl (optionally substituted by one, two or three substituents each independently selected from the group consisting of halogen, hydroxyl, C.sub.1-6alkyl, C.sub.2-6alkenyl, C.sub.2-6alkynyl, C.sub.1-6alkoxy, NR′R″, —C(O)—NR′R″, —C(O)—C.sub.1-6alkyl, —C(O)—C.sub.1-6alkoxy, —S(O).sub.w—C.sub.1-6alkyl (where w is 1, 2, or 3), NR′—S(O).sub.w, and —S(O).sub.w—NR′R″ (where w is 1, 2, or 3)), C.sub.3-6cycloalkyl, —S(O).sub.w—C.sub.1-6alkyl (where w is 1, 2, or 3), —S(O).sub.w—NR′R″ (where w is 1, 2, or 3), and —NR′—S(O) (where w is 1, 2, or 3)), 5-6 membered heteroaryl having one, two, or three heteroatoms each independently selected from O, N, and S (wherein the 5-6 membered heteroaryl may be optionally substituted on a carbon with one, two, three or more substituents selected from the group consisting of halogen, hydroxyl, nitro, cyano, carboxy, C.sub.1-6alkyl, C.sub.2-6alkenyl, C.sub.2-6alkynyl, C.sub.1-6alkoxy, phenyl (optionally substituted by one, two or three substituents each independently selected from the group consisting of halogen, hydroxyl, C.sub.1-6alkyl, C.sub.2-6alkenyl, C.sub.2-6alkynyl, C.sub.1-6alkoxy, NR′R″, —C(O)—NR′R″, —C(O)—C.sub.1-6alkyl, —C(O)—OH, —C(O)—C.sub.1-6alkoxy, —S(O).sub.w—C.sub.1-6alkyl (where w is 1, 2, or 3), —NR′—S(O).sub.w, —S(O).sub.w—NR′R″ (where w is 1, 2, or 3)), heteroaryl, heterocycle, NR′R″, —C(O)—NR′R″, —C(O)—C.sub.1-6alkyl, —C(O)—C.sub.1-6alkoxy, —S(O).sub.w—C.sub.1-6alkyl (where w is 1, 2, or 3), —NR′—S(O).sub.w, and —S(O).sub.w—NR′R″ (where w is 1, 2, or 3), and on a nitrogen by R′), C.sub.1-6alkyl, C.sub.1-6alkoxy, C.sub.2-6alkenyl, C.sub.3-10cycloalkyl (optionally substituted with one, two, three, or more substituents selected from the group consisting of halogen, hydroxyl, nitro, cyano, carboxy, NR′R″, —C(O)—NR′R″, ═CNR′, C.sub.1-6alkyl, C.sub.1-6alkoxy, —C(O)—C.sub.1-6alkyl, and —C(O)—C.sub.1-6alkoxy, and wherein the C.sub.3-10cycloalkyl may optionally be a bridged cycloalkyl)), and a 4-6 membered heterocycloalkyl having one or two heteroatoms each independently selected from O, N, and S (wherein the 4-6 membered heterocycloalkyl may be optionally substituted with one, two, three, or more substituents selected from the group consisting of halogen, hydroxyl, nitro, cyano, carboxy, NR′R″, —C(O)—NR′R″, C.sub.1-6alkyl, C.sub.1-6alkoxy, —C(O)—C.sub.1-6alkyl, and —C(O)—C.sub.1-6alkoxy);

[0175] R′ is selected, independently for each occurrence, from H, methyl, ethyl, propyl, phenyl, and benzyl;

[0176] R″ is selected, independently for each occurrence, from H, methyl, ethyl, propyl, butyl, carboxybenzyl, —C(O)-methyl and —C(O)-ethyl, or R′ and R″ taken together may form a 4-6 membered heterocycle;

[0177] each of moieties R.sup.4, R.sup.5, R.sup.6, R.sup.7, R.sup.8, R.sup.9, R.sup.10, and R.sup.11 are independently selected for each occurrence from the group consisting of hydrogen, C.sub.1-6alkyl, C.sub.2-6alkenyl, C.sub.2-6alkynyl, halogen, hydroxyl, nitro, cyano, NR′R″, —C(O)—NR′R″, —S(O).sub.w—C.sub.1-6alkyl (where w is 1, 2, or 3), —NR′—S(O).sub.w, and —S(O).sub.w—NR′R″ (where w is 0, 1, or 2), C.sub.1-6alkoxy, —C(O)—OH, —C(O)—C.sub.1-6alkyl, and —C(O)—C.sub.1-6alkoxy;

[0178] wherein for each occurrence, C.sub.1-6alkyl may be optionally substituted with one, two, three, or more substituents selected from the group consisting of halogen, hydroxyl, nitro, cyano, carboxy, C.sub.2-6alkenyl, C.sub.2-6alkynyl, C.sub.1-6alkoxy, phenyl, NR′R″, —C(O)—NR′R″, S(O).sub.w-methyl (where w is 1, 2, or 3), —NR′—S(O).sub.w, and S(O).sub.w—NR′R″ (where w is 0, 1, or 2); C.sub.1-6alkoxy may be optionally substituted with one, two, three, or more substituents selected from the group consisting of halogen, hydroxyl, nitro, cyano, carboxy, C.sub.1-6alkyl, phenyl, NR′R″, —C(O)—NR′R″, S(O).sub.w—C.sub.1-6alkyl (where w is 1, 2 or 3), —NR′—S(O).sub.w, and S(O).sub.w—NR′R″ (where w is 0, 1, or 2); and C.sub.3-6cycloalkyl may be optionally substituted with one, two, three, or more substituents selected from the group consisting of halogen, hydroxyl, nitro, cyano, carboxy, C.sub.1-6alkyl, C.sub.1-6alkoxy, —C(O)—C.sub.1-6alkyl, —C(O)—C.sub.1-6alkoxy, and NR′R″; and pharmaceutically acceptable salts thereof.

[0179] In one illustrative embodiment of the disclosure, compounds described in International Patent Publication WO2001/068641 can be used, including a compound having the formula:

##STR00034##

[0180] In another illustrative embodiment of the disclosure, compounds described in International Patent Publication WO2013/019967, including a compound having the formula

##STR00035##

or a pharmaceutically acceptable salt thereof is described, wherein
Ar.sup.1 is selected from the group consisting of phenyl, 2-pyridyl, 3-pyridyl, and 4-pyridyl;
R.sup.1 is hydrogen or pro-drug forming group;
Ak is alkylene;

Z is

[0181] ##STR00036##

where X is CHN.sub.3, C═O, C═NR.sup.5, —C(O)N(R.sup.N)—, or NR.sup.N, where R.sup.5 is hydroxy or a derivative thereof or amino or a derivative thereof, and R.sup.N is selected from the group consisting of alkyl, alkenyl, alkynyl, heteroalkyl, arylalkyl, heteroarylalkyl, alkyl-C(O), heteroalkyl-C(O), alkoxyl-C(O), alkynyl-C(O), alkylacylamino-C(O), and heteroalkylacylamino-C(O), each of which is optionally substituted;
R.sup.4 is alkyl, heteroalkyl, alkenyl, or alkynyl, each of which is optionally substituted;

Y is O or HN;

[0182] R.sup.A represents from 0 to 3 substituents independently in each instance, halo or selected from the group consisting of alkyl, heteroalkyl, aryl, heteroaryl, amino and derivatives thereof, and hydroxyl and derivatives thereof, each of which is optionally substituted; and
R.sup.B represents from 0 to 3 substituents independently in each instance, halogen or selected from the group consisting of alkyl, heteroalkyl, aryl, heteroaryl, amino and derivatives thereof, and hydroxyl and derivatives thereof, each of which is optionally substituted.

[0183] In another illustrative embodiment of the disclosure, compounds described in Bourne et al. (2008) J. Vir. 82(20):10262-10270 and in International Patent Publication WO2013/019967 can be used in accordance with the methods of the disclosure, including a compound having the formula

##STR00037##

or a pharmaceutically acceptable salt thereof is described, wherein;
Ar.sup.2 is aryl or heteroaryl each of which is optionally substituted;
R.sup.1 is independently in each instance selected from the group consisting of hydrogen and pro-drug forming group;
R.sup.4 is alkyl, heteroalkyl, alkenyl, or alkynyl, each of which is optionally substituted;

Y is O or HN;

[0184] R.sup.6 is in each instance independently selected from the group consisting of hydrogen and Ak-Z, where Ak is alkylene, and Z is independently in each instance hydrogen or NR.sub.2R.sub.3; where R.sub.2 and R.sub.3 are independently in each instance hydrogen, or selected from the group consisting of alkyl, cycloalkyl, heteroalkyl and heterocycloalkyl, each of which is optionally substituted, or R.sub.2 and R.sub.3 are taken together with the attached nitrogen to form

##STR00038##

wherein X is CHN.sub.3, C═O, —C(O)N(R.sup.Na)—, C═NR.sup.5, or NR.sup.Na where R.sup.5 is hydroxy or a derivative thereof or amino or a derivative thereof; and RN.sup.a is selected from the group consisting of hydrogen, and alkyl, alkenyl, alkynyl, heteroalkyl, arylalkyl, heteroarylalkyl, alkyl-C(O), heteroalkyl-C(O), alkoxyl-C(O), alkynyl-C(O), alkylacylamino-C(O), and heteroalkylacylamino-C(O), each of which is optionally substituted;
Ak.sup.1 is (CH.sub.2)n, where n is 1 to 4;
R.sup.A represents from 0 to 3 substituents independently in each instance, halo or selected from the group consisting of alkyl, heteroalkyl, aryl, heteroaryl, amino and derivatives thereof, and hydroxyl and derivatives thereof, each of which is optionally substituted; and
R.sup.B represents from 0 to 3 substituents independently in each instance, halogen or selected from the group consisting of alkyl, heteroalkyl, aryl, heteroaryl, amino and derivatives thereof, and hydroxyl and derivatives thereof, each of which is optionally substituted.

[0185] In another illustrative embodiment, compounds described in International Patent Publication WO2010/069147 can be used in accordance with the methods of the disclosure.

[0186] In another illustrative embodiment, compounds described in International Patent Publication WO2013/102655 can be used in accordance with the methods of the disclosure. For example, the present disclosure provides compounds, which can be represented by the formula I:

##STR00039##

including any possible stereoisomers or tautomeric forms thereof, wherein:
B is selected from the group comprising C.sub.1-C.sub.3alkyl optionally substituted with one or more fluoro atoms;
Z is selected from H or halogen; or B and Z together with the carbons to which they are attached form a 4-7 membered ring, optionally containing one or more heteroatoms, wherein the 4-7 membered ring optionally is substituted with one or more substituents selected from the group comprising C.sub.1-C.sub.3alkyl, oxo, OH and halogen;
R.sub.1 is selected from the group comprising heteroaryl and phenyl, optionally substituted with one or more substituents selected from the group comprising halogen and C.sub.1-C.sub.3alkyl;
R.sub.2 is selected from the group comprising —R.sub.6-R.sub.7, C≡N, cyclopropyl, and CF.sub.3;
R.sub.3 is selected from the group comprising C.sub.1-C.sub.3alkoxycarbonyl, and C≡N;
R.sub.4 and R.sub.5 independently are selected from the group comprising H, methyl and halogen;
R.sub.6 is C.sub.1-C.sub.3alkyl, C.sub.2-C.sub.3alkenyl, both optionally substituted with one or more fluoro;
R.sub.7 is selected from the group comprising hydrogen, a hetero C.sub.3-7cycloalkyl, cyclopropyl, C.sub.1-C.sub.3alkoxy and CF.sub.3;
or a pharmaceutically acceptable salt or a solvate thereof.
B for example may be selected from the group comprising C.sub.1-C.sub.3alkyl optionally substituted with one or more fluoro atoms; Z is selected from H or halogen; or B and Z together with the carbons to which they are attached form a 4-7 membered ring, optionally containing one or more heteroatoms, wherein the 4-7 membered ring optionally is substituted with one or more substituents selected from the group comprising C.sub.1-C.sub.3alkyl, oxo and halogen;
R.sub.1 is selected from the group comprising heteroaryl and phenyl, optionally substituted with one or more substituents selected from the group comprising halogen and C.sub.1-C.sub.3alkyl;
R.sub.2 is selected from the group comprising —R.sub.6-R.sub.7, C≡N, cyclopropyl, and CF.sub.3;
R.sub.3 is selected from the group comprising C.sub.1-C.sub.3alkoxycarbonyl, and C≡N;
R.sub.4 and R.sub.5 independently are selected from the group comprising H and halogen;
R.sub.6 is C.sub.1-C.sub.3alkyl, optionally substituted with fluoro;
and R.sub.7 is selected from the group comprising hydrogen, a hetero C.sub.3-7cycloalkyl, cyclopropyl and CF.sub.3; and/or pharmaceutically acceptable salt or solvate thereof.
In a particular group of compounds of Formula I according to the invention, B is selected from the group comprising C.sub.1-C.sub.3alkyl optionally substituted with one or more fluoro atoms;
Z is selected from H or halogen; or B and Z together with the carbons to which they are attached form a 4-7 membered ring, optionally containing one or more heteroatoms, wherein the 4-7 membered ring optionally is substituted with one or more substituents selected from the group comprising C.sub.1-C.sub.3alkyl;
R.sub.1 is selected from the group comprising heteroaryl and phenyl, optionally substituted with 1 or more halogen atoms;

[0187] In yet another aspect, the disclosure relates to a compound according to formula:

##STR00040##

wherein B, Z, R.sub.1 R.sub.3, R.sub.4, and R.sub.5 are defined as above.

[0188] In another illustrative embodiment, compounds described in International Patent Publication No. WO2014/037480 can be used in accordance with the methods of the invention. For example, the present disclosure provides compounds, which can be represented by (i) novel compounds having the general formula I:

##STR00041##

wherein
R.sup.1 is C.sub.1-6alkyl or trifluoromethyl-C.sub.xH.sub.2x—, wherein x is 1-6;
one of R.sup.2 and R.sup.3 is phenyl, which is once or twice or three times substituted by C.sub.1-6alkyl, cyano or halogen; and the other one is hydrogen or deuterium;
R.sup.4 is phenyl, thiazolyl, oxazolyl, imidazolyl, thienyl or pyridinyl, which is unsubstituted or substituted by C.sub.1-6alkyl, C.sub.1-6alkylsulfanyl, halogen or cycloalkyl, where said C.sub.1-6alkyl can be further optionally substituted with halogen;

A is

[0189] ##STR00042##

which is unsubstituted or substituted by groups selected from C.sub.1-6alkyl, deuterium and halogen; or pharmaceutically acceptable salts, or enantiomers, or diastereomers thereof.

[0190] A further embodiment of the present disclosure is (ii) a compound of formula I, wherein

R.sup.1 is methyl, ethyl, propyl, isopropyl, tert-butyl or trifluoromethylmethyl; one of R.sup.2 and R.sup.3 is phenyl, which is once or twice or three times substituted by fluoro, chloro, bromo, iodo, methyl, or cyano; and the other one is hydrogen or deuterium;
R.sup.4 is phenyl, thiazolyl, oxazolyl, imidazolyl, thienyl or pyridinyl, which is unsubstituted or substituted by methyl, isopropyl, tert-butyl, bifluoromethyl, trifluoromethyl, cyclopropyl, methylsulfanyl, fluoro or chloro;

A is

[0191] ##STR00043##

which is unsubstituted or substituted by groups selected from methyl, isopropyl, deuterium and fluoro; or pharmaceutically acceptable salts, or enantiomers, or diastereomers thereof.

[0192] Another embodiment of the present disclosure is (iii) a compound of formula I, wherein

R.sup.1 is C.sub.1-6alkyl or trifluoromethyl-C.sub.xH.sub.2x—, wherein x is 1-6;
one of R.sup.2 and R.sup.3 is phenyl, which is once or twice or three times substituted by C.sub.1-6alkyl, cyano or halogen; and the other one is hydrogen or deuterium;
R.sup.4 is phenyl, thiazolyl, oxazolyl, imidazolyl, thienyl or pyridinyl, which is unsubstituted or substituted by C.sub.1-6alkyl, C.sub.1-6alkylsulfanyl, halogen or cycloalkyl, where said C.sub.1-6alkyl can be further optionally substituted with halogen;

A is

[0193] ##STR00044##

which is unsubstituted or substituted by groups selected from C.sub.1-6alkyl, deuterium and halogen; or pharmaceutically acceptable salts, or enantiomers, or diastereomers thereof.

[0194] A further embodiment of the present disclosure is (iv) a compound of formula I, wherein

R.sup.1 is methyl, ethyl, propyl, isopropyl or trifluoromethylmethyl;
one of R.sup.2 and R.sup.3 is phenyl, which is once or twice or three times substituted by fluoro, chloro, bromo, iodo, methyl or cyano; and the other one is hydrogen or deuterium;
R.sup.4 is phenyl, thiazolyl, oxazolyl, imidazolyl, thienyl or pyridinyl, which is unsubstituted or substituted by methyl, isopropyl, trifluoromethyl, cyclopropyl, methylsulfanyl, fluoro or chloro;

A is

[0195] ##STR00045##

which is unsubstituted or substituted by groups selected from methyl, isopropyl, deuterium and fluoro; or pharmaceutically acceptable salts, or enantiomers, or diastereomers thereof.

[0196] Another embodiment of the present disclosure is (v) a compound of formula I, wherein

R.sup.1 is C.sub.1-6alkyl;
one of R.sup.2 and R.sup.3 is phenyl, which is once or twice or three times substituted by C.sub.1-6alkyl or halogen; and the other one is hydrogen or deuterium;
R.sup.4 is thiazolyl, oxazolyl, imidazolyl, thienyl or pyridinyl, which is unsubstituted or substituted by C.sub.1-6alkyl, halogen or cycloalkyl, where said C.sub.1-6alkyl can be further optionally substituted with halogen;

A is

[0197] ##STR00046##

which is unsubstituted or substituted by groups selected from C.sub.1-6alkyl, deuterium and halogen;
or pharmaceutically acceptable salts, or enantiomers, or diastereomers thereof.

[0198] A further embodiment of the present disclosure is (vi) a compound of formula I, wherein

R.sup.1 is methyl or ethyl;
one of R.sup.2 and R.sup.3 is phenyl, which is once or twice or three times substituted by fluoro, chloro, bromo, iodo or methyl; and the other one is hydrogen or deuterium;
R.sup.4 is thiazolyl, oxazolyl, imidazolyl, thienyl or pyridinyl, which is unsubstituted or substituted by methyl, isopropyl, trifluoromethyl, cyclopropyl or fluoro;

A is

[0199] ##STR00047##

which is unsubstituted or substituted by groups selected from methyl, isopropyl, deuterium and fluoro;
or pharmaceutically acceptable salts, or enantiomers, or diastereomers thereof.

[0200] Another embodiment of the present disclosure is (vii) a compound of formula I or pharmaceutically acceptable salts, or enantiomers, or diastereomers thereof, wherein

R.sup.1 is C.sub.1-6alkyl;
one of R.sup.2 and R.sup.3 is phenyl, which is once or twice or three times substituted by halogen; and the other one is hydrogen;

R.SUP.4 .is

[0201] ##STR00048##

A is

[0202] ##STR00049##

which is unsubstituted or substituted by C.sub.1-6alkyl.

[0203] A further embodiment of the present disclosure is (viii) a compound of formula I or pharmaceutically acceptable salts, or enantiomers, or diastereomers thereof, wherein R.sup.1 is methyl or ethyl;

one of R.sup.2 and R.sup.3 is phenyl, which is once or twice or three times substituted by fluoro, chloro or bromo; and the other one is hydrogen;

R.SUP.4 .is

[0204] ##STR00050##

A is

[0205] ##STR00051##

which is unsubstituted or substituted by methyl.

[0206] A further embodiment of the present disclosure is (ix) a compound of formula I or pharmaceutically acceptable salts, or enantiomers, or diastereomers thereof, wherein

R.sup.1 is methyl or ethyl;
one of R.sup.2 and R.sup.3 is

##STR00052##

and the other one is hydrogen, wherein
A.sup.1 is hydrogen or fluoro;
A.sup.2 is hydrogen or fluoro;
A.sup.3 is fluoro, chloro or bromo; provided that at least one of A.sup.1 and A.sup.2 is hydrogen;

R.SUP.4 .is

[0207] ##STR00053##

A is

[0208] ##STR00054##

wherein A.sup.4 is hydrogen or methyl.

[0209] Another embodiment of the present disclosure is (x) a compound of formula I, wherein

R.sup.1 is C.sub.1-6alkyl or trifluoromethyl-C.sub.xH.sub.2x—, wherein x is 1-6;
one of R.sup.2 and R.sup.3 is phenyl, which is once or twice or three times substituted by C.sub.1-6alkyl, cyano or halogen; and the other one is hydrogen or deuterium;
R.sup.4 is thiazolyl, oxazolyl, imidazolyl, thienyl or pyridinyl, which is unsubstituted or substituted by C.sub.1-6alkyl, C.sub.1-6alkylsulfanyl, halogen or cycloalkyl, where said C.sub.1-6alkyl can be further optionally substituted with halogen;

A is

[0210] ##STR00055##

which is unsubstituted or substituted by groups selected from C.sub.1-6alkyl, deuterium and halogen; or pharmaceutically acceptable salts, or enantiomers, or diastereomers thereof.

[0211] A further embodiment of the present disclosure is (xi) a compound of formula I, wherein

R.sup.1 is methyl, ethyl, propyl, isopropyl or trifluoromethylmethyl;
one of R.sup.2 and R.sup.3 is phenyl, which is once or twice or three times substituted by fluoro, chloro, bromo, methyl, or cyano; and the other one is hydrogen or deuterium;
R.sup.4 is thiazolyl, oxazolyl, imidazolyl, thienyl or pyridinyl, which is unsubstituted or substituted by methyl, isopropyl, trifluoromethyl, cyclopropyl, methylsulfanyl, fluoro or chloro;

A is

[0212] ##STR00056##

which is unsubstituted or substituted by groups selected from methyl, deuterium and fluoro;
or pharmaceutically acceptable salts, or enantiomers, or diastereomers thereof.

[0213] Another embodiment of the present disclosure is (xii) a compound of formula I, wherein

R.sup.1 is C.sub.1-6alkyl or trifluoromethyl-C.sub.xH.sub.2x—, wherein x is 1-6;
one of R.sup.2 and R.sup.3 is phenyl, which is once or twice or three times substituted by C.sub.1-6alkyl or halogen; and the other one is hydrogen or deuterium;
R.sup.4 is thiazolyl, oxazolyl, imidazolyl, thienyl or pyridinyl, which is unsubstituted or substituted by C.sub.1-6alkyl, C.sub.1-6alkylsulfanyl, halogen or cycloalkyl, where said C.sub.1-6alkyl can be further optionally substituted with halogen;

A is

[0214] ##STR00057##

which is unsubstituted or substituted by groups selected from C.sub.1-6alkyl, deuterium and halogen;
or pharmaceutically acceptable salts, or enantiomers, or diastereomers thereof.

[0215] A further embodiment of present disclosure is (xiii) a compound of formula I, wherein

R.sup.1 is methyl, ethyl, isopropyl or trifluoromethylmethyl;
one of R.sup.2 and R.sup.3 is phenyl, which is once or twice or three times substituted by fluoro, chloro, bromo, iodo or methyl; and the other one is hydrogen or deuterium;
R.sup.4 is thiazolyl, oxazolyl, imidazolyl, thienyl or pyridinyl, which is unsubstituted or substituted by methyl, isopropyl, trifluoromethyl, cyclopropyl, methylsulfanyl or fluoro;

A is

[0216] ##STR00058##

which is unsubstituted or substituted by groups selected from methyl, isopropyl, deuterium and fluoro;
or pharmaceutically acceptable salts, or enantiomers, or diastereomers thereof.

[0217] Another embodiment of the present disclosure is (xiv) a compound of formula I or pharmaceutically acceptable salts, or enantiomers, or diastereomers thereof, wherein

R.sup.1 is C.sub.1-6alkyl or trifluoromethyl-C.sub.xH.sub.2x—, wherein x is 1-6;
one of R.sup.2 and R.sup.3 is phenyl, which is once or twice or three times substituted by halogen; and
the other one is hydrogen or deuterium;

R.SUP.4 .is

[0218] ##STR00059##

which is unsubstituted or substituted by C.sub.1-6alkyl, C.sub.1-6alkylsulfanyl or cycloalkyl, where said C.sub.1-6alkyl can be further optionally substituted with halogen;

A is

[0219] ##STR00060##

which is unsubstituted or substituted by groups selected from C.sub.1-6alkyl, deuterium and halogen.

[0220] A further embodiment of the present disclosure is (xv) a compound of formula I or pharmaceutically acceptable salts, or enantiomers, or diastereomers thereof, wherein

R.sup.1 is methyl, ethyl, propyl, isopropyl or trifluoromethylmethyl;
one of R.sup.2 and R.sup.3 is phenyl, which is once or twice or three times substituted by fluoro, chloro, bromo or iodo; and the other one is hydrogen or deuterium;

R.SUP.4 .is

[0221] ##STR00061##

which is unsubstituted or substituted by methyl, isopropyl, trifluoromethyl, cyclopropyl or methylsulfanyl;

A is

[0222] ##STR00062##

which may be unsubstituted or substituted by groups selected from methyl, isopropyl, deuterium and fluoro.

[0223] Another embodiment of the present disclosure is (xvi) a compound of formula I or pharmaceutically acceptable salts, or enantiomers, or diastereomers thereof, wherein

R.sup.1 is C.sub.1-6alkyl;
one of R.sup.2 and R.sup.3 is

##STR00063##

and the other one is hydrogen;

R.SUP.4 .is

[0224] ##STR00064##

which is unsubstituted or substituted by C.sub.1-6alkyl;

A is

[0225] ##STR00065##

which is substituted by halogen.

[0226] Another embodiment of the present disclosure is (xvii) a compound of formula I or pharmaceutically acceptable salts, or enantiomers, or diastereomers thereof, wherein

R.sup.1 is C.sub.1-6alkyl;
one of R.sup.2 and R.sup.3 is phenyl, which is substituted by halogen; and the other one is hydrogen;

R.SUP.4 .is

[0227] ##STR00066##

which is substituted by C.sub.1-6alkyl;

A is

[0228] ##STR00067##

which may be unsubstituted or substituted by halogen.

[0229] Another embodiment of the present disclosure is (xviii) a compound of formula 1b or pharmaceutically acceptable salts, or enantiomers, or diastereomers thereof,

##STR00068##

wherein
R.sup.1 is C.sub.1-6alkyl;
one of R.sup.2 and R.sup.3 is phenyl, which is twice or thrice substituted by cyano or halogen; and the other one is hydrogen or deuterium;
R.sup.4 is phenyl, thiazolyl, oxazolyl, imidazolyl, thienyl or pyridinyl; which is unsubstituted or once or twice substituted by C.sub.1-6alkyl, halogen, cycloalkyl or trifluoromethyl;
R.sup.5 is hydrogen;
R.sup.6 is hydrogen;

A is

[0230] ##STR00069##

which may be unsubstituted or one, two, three or four times substituted by deuterium or halogen.

[0231] Additional compounds suitable for use with the methods described herein are found in Campana et al. (2013) J. Virol. 87(12):6931 and in International Patent Publication No. WO2013/006394, including, e.g., a compound having formula (I):

##STR00070##

wherein
R.sup.1 is hydrogen;
R.sup.2 is selected from the group consisting of hydrogen, methyl, trifluoromethyl, fluorine, and chlorine;
R.sup.3 is selected from the group consisting of hydrogen, methyl, fluorine, and chlorine;
R.sup.4 is selected from the group consisting of hydrogen, fluorine, chlorine, and methyl;
R.sup.5 is selected from the group consisting of hydrogen and chlorine;
R.sup.7 is selected from the group consisting of hydrogen, chlorine, fluorine, and bromine;
R.sup.9 is selected from the group consisting of hydrogen, methyl, fluorine, and chlorine;
R.sup.9 is selected form a group consisting of NH.sub.2,

##STR00071## ##STR00072##

or a pharmaceutically acceptable salt form thereof.

[0232] Additional compounds of the disclosure are found in International Patent Publication No. WO2013/096744, including, e.g., a compound having formula (IV):

Formula IV:

[0233] ##STR00073##

or pharmaceutically acceptable salts thereof wherein
R.sup.4 is H or C.sub.1-C.sub.3alkyl;
wherein each R.sup.5 is independently selected at each occurrence from the group consisting of CH.sub.3, C.sub.1-C.sub.6alkoxy, halo, —CN, —NO.sub.2, -(L).sub.m-SR.sup.9, -(L).sub.m-S(═O)R.sup.9, -(L).sub.m-S(═O).sub.2R.sup.9, -(L).sub.m-NHS(═O).sub.2R.sup.9, -(L).sub.m-C(═O)R.sup.9, -(L).sub.m-OC(═O)R.sup.9, -(L).sub.m-CO.sub.2R.sup.8, -(L).sub.m-OCO.sub.2R.sup.8, -(L).sub.m-N(R.sup.8).sub.2, -(L).sub.m-C(═O)N(R.sup.8).sub.2, -(L).sub.m-OC(═O)N(R.sup.8).sub.2, -(L).sub.m-NHC(═O)NH(R.sup.8), -(L).sub.m-NHC(═O)R.sup.9, -(L).sub.m-NHC(═O)OR.sup.9, -(L).sub.m-C(OH)(R.sup.8).sub.2, -(L).sub.mC(NH.sub.2)(R.sup.8).sub.2, —C.sub.1-C.sub.6haloalkyl, —C.sub.1-C.sub.6dihaloalkyl and —C.sub.1-C.sub.6trihaloalkyl;
L is independently, at each occurrence, a bivalent radical selected from —(C.sub.1-C.sub.3alkylene)-, —(C.sub.3-C.sub.7cycloalkylene)-, —(C.sub.1-C.sub.3alkylene).sub.m-O—(C.sub.1-C.sub.3alkylene).sub.m-, or —(C.sub.1-C.sub.3alkylene).sub.m-NH—(C.sub.1-C.sub.3 alkylene).sub.m-;
each R.sup.8 is independently, at each occurrence, H, C.sub.1-C.sub.6alkyl, —C.sub.1-C.sub.6haloalkyl, —C.sub.1-C.sub.6dihaloalkyl, —C.sub.1-C.sub.6trihaloalkyl, C.sub.1-C.sub.6heteroalkyl, C.sub.3-C.sub.10cycloalkyl, C.sub.3-C.sub.10heterocycloalkyl, aryl, heteroaryl, —C.sub.1-C.sub.4alkyl-(C.sub.3-C.sub.10cycloalkyl), —C.sub.1-C.sub.4alkyl-(C.sub.3-C.sub.10heterocycloalkyl), —C.sub.1-C.sub.4alkyl-(aryl), or —C.sub.1-C.sub.4alkyl(heteroaryl), and wherein the alkyl, heteroalkyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl is optionally substituted with 1-5 substituents selected from R.sup.2;
R.sup.9 is —C.sub.1-6alkyl, —C.sub.1-6haloalkyl, —C.sub.1-6dihaloalkyl, —C.sub.1-6trihaloalkyl, C.sub.1-C.sub.6heteroalkyl, C.sub.3-C.sub.10cycloalkyl, C.sub.3-C.sub.10heterocycloalkyl, aryl, heteroaryl, —C.sub.1-C.sub.4alkyl-(C.sub.3-C.sub.10cycloalkyl), —C.sub.1-C.sub.4alkyl-(C.sub.3-C.sub.10heterocycloalkyl), —C.sub.1-C.sub.4alkyl-(aryl), or —C.sub.1-C.sub.4alkyl-(heteroaryl), and wherein the alkyl, heteroalkyl, cycloalkyl, heterocycloalkyl, aryl or heteroaryl ring is optionally substituted with 0-5 substituents selected from R.sup.2;
R.sup.10 is OH, —C.sub.1-6alkyl, C.sub.1-6alkyl —OH, —C.sub.1-6haloalkyl, —C.sub.1-6dihaloalkyl, —C.sub.1-6trihaloalkyl, C.sub.1-6alkyl heteroalkyl, C.sub.3-C.sub.10cycloalkyl, —C.sub.3-C.sub.10heterocycloalkyl, aryl, heteroaryl, —C.sub.1-C.sub.4alkyl-(C.sub.3-C.sub.10cycloalkyl), —C.sub.1-C.sub.4alkyl-(C.sub.3-C.sub.10heterocycloalkyl), —C.sub.1-C.sub.4alkyl-(aryl), or —C.sub.1-C.sub.4alkyl-(heteroaryl), and wherein the alkyl, heteroalkyl, cycloalkyl, heterocycloalkyl, aryl or heteroaryl ring is optionally substituted with 1-5 substituents selected from R.sup.2;
R.sup.11 is a bond or C.sub.1-3alkylene, wherein the C.sub.1-3alkylene is optionally substituted with 1-3 substituents selected from R.sup.2;
R.sup.2 is independently selected at each occurrence from the group consisting of halo, —CN, —NO.sub.2, —C.sub.1-6 alkyl, —C.sub.1-6alkoxy, —C.sub.1-6alkylhaloalkyl, —C.sub.1-6dihaloalkyl, —C.sub.1-6trihaloalkyl, —C.sub.1-6heteroalkyl, and C(O)—C.sub.1-6 alkyl;
w is 0, 1 or 2;
each occurrence of x is independently selected from the group consisting of 0, 1, 2, 3, and 4;
each occurrence of y is independently selected from the group consisting of 1, 2, and 3;
each occurrence of z is independently selected from the group consisting of 0, 1, 2, and 3;
each occurrence of m is independently 0, 1, or 2.

[0234] Additional compounds of the disclosure are found in International Patent Publication No. WO2014/033167, including, e.g., a compound of Formula (I)

##STR00074##

or a stereoisomer or tautomeric form thereof, wherein:
A represents N, C or O;
B represents C or N;
D represents C or N;
E represents C or N; wherein if A and E are either N or C, they are optionally substituted with R.sup.4;
R.sub.1 represents hydrogen or C.sub.1-3alkyl;
R.sub.2 represents C.sub.1-6alkyl, C.sub.1-3alkyl-R.sub.6, benzyl, or a 3-7 membered saturated ring optionally containing one or more heteroatoms each independently selected from the group consisting of O, S and N, such C.sub.1-6alkyl or a 3-7 membered saturated ring optionally being substituted with one or more substituents each independently selected from the group consisting of hydrogen, halo, C.sub.1-3alkyloxy, C.sub.1-4alkyl, OH, CN, CFH.sub.2, CF.sub.2H or CF.sub.3; or R.sub.1 R.sub.2 together with the nitrogen to which they are attached form a 5-7 membered saturated ring optionally being substituted with one or more substituents each independently selected from the group consisting of hydrogen, halogen, C.sub.1-4alkyloxy, C.sub.1-3alkyl, OH, CN, CFH.sub.2, CF.sub.2H and CF.sub.3;
Each R.sub.3 is independently selected from hydrogen, halo, C.sub.1-4alkyloxy, C.sub.1-4alkyl, OH, CN, CFH.sub.2, CF.sub.2H, CF.sub.3 or a 3-5 membered saturated ring optionally containing one or more heteroatoms each independently selected from the group consisting of O and N;
R.sub.4 represents hydrogen, C.sub.1-4alkyl, C.sub.3-5cycloalkyl, —(C═O)C.sub.1-4-alkyl, —(C═O)—C.sub.1-3alkyloxy or in case A or E equals C, R.sub.4 in addition can be halogen;
R.sub.5 represents hydrogen or halogen;
R.sub.6 represents a 3-7 membered saturated ring optionally containing one or more heteroatoms each independently selected from the group consisting of O, S, and N, such 3-7 membered saturated ring optionally being substituted with one or more substituents each independently selected from the group consisting of hydrogen, halo, C.sub.1-3alkyloxy, C.sub.1-4alkyl, OH, CN, CFH.sub.2, CF.sub.2H, CF.sub.3; or a pharmaceutically acceptable salt or a solvate thereof.

[0235] Additional compounds of the disclosure are found in International Patent Publication No. WO2014/033170, including, e.g., a compound of Formula (Ia)

##STR00075##

or a stereoisomer or tautomeric form thereof, wherein:
B represents a monocyclic 5 to 6 membered aromatic ring, optionally containing one or more heteroatoms each independently selected from the group consisting of O, S, and N, such 5 to 6 membered aromatic ring optionally being substituted with one or more substituents each independently selected from the group consisting of hydrogen, halo, —C.sub.1-C.sub.3alkyl, CN, CFH.sub.2, CF.sub.2H and CF.sub.3;
R.sub.1 represents hydrogen or —C.sub.1-3alkyl;
R.sub.2 represents —C.sub.1-6alkyl, —C.sub.1-3alkyl-R.sub.5, benzyl, —C(═O)—R.sub.5, CFH.sub.2, CF.sub.2H, CF.sub.3, or a 3-7 membered saturated ring optionally containing one or more heteroatoms each independently selected from the group consisting of O, S, and N, such 3-7 membered saturated ring or C.sub.1-6alkyl optionally being substituted with one or more substituents each independently selected from the group consisting of hydrogen, halo, —C.sub.1-4alkyloxy, oxo, —C(═O)—C.sub.1-3alkyl, —C.sub.1-4alkyl, OH, CN, CFH.sub.2, CF.sub.2H and CF.sub.3; or R.sub.1 and R.sub.2 together with the nitrogen to which they are attached form a 1,4-dioxa-8-azaspiro[4.5] moiety or a 5-7 membered saturated ring, optionally containing one or more additional heteroatoms each independently selected from the group consisting of O, S, and N, such 5-7 membered saturated ring optionally being substituted with one or more substituents each independently selected from the group consisting of hydrogen, halo, C.sub.1-4alkyloxy, oxo, —C(═O)—C.sub.1-3alkyl, —C.sub.1-4alkyl, OH, CN, CFH.sub.2, CF.sub.2H and CF.sub.3; each R.sub.4 is independently selected from hydrogen, halo, —C.sub.1-4alkyloxy, —C.sub.1-4alkyl, OH, CN, CFH.sub.2, CF.sub.2H, CF.sub.3 or a 3-5 membered saturated ring optionally containing one or more heteroatoms each independently selected from the group consisting of O and N;
R.sub.5 represents —C.sub.1-6alkyl, CFH.sub.2, CF.sub.2H, CF.sub.3 or a 3-7 membered saturated ring optionally containing one or more heteroatoms each independently selected from the group consisting of O, S and N, such 3-7 membered saturated ring optionally being substituted with one or more substituents each independently selected from the group consisting of hydrogen, halo, C.sub.1-4alkyloxy, oxo, —C(═O)—C.sub.1-3alkyl, C.sub.1-4alkyl, OH, CN, CFH.sub.2, CF.sub.2H and CF.sub.3; or a pharmaceutically acceptable salt or a solvate thereof.

[0236] Additional compounds of the disclosure are found in International Patent Publication No. WO2014/033176, including, e.g., a compound of Formula I:

##STR00076##

or a stereoisomer or tautomeric form thereof, wherein:
B represents a monocyclic 5 to 6 membered aromatic ring, optionally containing one or more heteroatoms each independently selected from the group consisting of O, S, and N, such 5 to 6 membered aromatic ring optionally being substituted with one or more substituents each independently selected from the group consisting of hydrogen, halogen, —C.sub.1-3alkyl, CN, CFH.sub.2, CF.sub.2H and CF.sub.3;
R.sub.1 represents hydrogen or —C.sub.1-3alkyl;
R.sub.2 represents —C.sub.1-6alkyl, —C.sub.1-6alkenyl, —C.sub.1-6alkyl-R.sub.5, —C(═O)—R.sub.5, CFH.sub.2, CF.sub.2H, CF.sub.3, a dihydro-indenyl or tetrahydronaphthalenyl moiety optionally substituted with OH, or a 3-7 membered saturated ring optionally containing one or more heteroatoms each independently selected from the group consisting of O, S, and N, such 3-7 membered saturated ring, —C.sub.1-6alkyl-R.sub.5 or —C.sub.1-6alkyl optionally being substituted with one or more substituents each independently selected from the group consisting of hydrogen, halogen, —C.sub.1-4alkyloxy, —C.sub.1-4alkyloxycarbonyl, oxo, —C(═O)—C.sub.1-3alkyl, —C.sub.1-4alkyl, OH, CN, CFH.sub.2, CF.sub.2H and CF.sub.3; or R.sub.1 and R.sub.2 together with the nitrogen to which they are attached form a 6-10 membered bicyclic or bridged ring or a 5-7 membered saturated ring, such bicyclic, bridged or saturated ring moiety optionally containing one or more additional heteroatoms each independently selected from the group consisting of O, S, and N, such 5-7 membered saturated ring optionally being substituted with one or more substituents each independently selected from the group consisting of hydrogen, halogen, C.sub.1-4alkyloxy, C.sub.1-4alkyloxycarbonyl, oxo, C(═O)—C.sub.1-3alkyl, —C.sub.1-4alkyl, OH, CN, CFH.sub.2, CF.sub.2H and CF.sub.3; each R.sub.4 is independently selected from hydrogen, halo, C.sub.1-4alkyloxy, C.sub.1-4alkyl, C.sub.1-4alkenyl, OH, CN, CFH.sub.2, CF.sub.2H, CF.sub.3, HC≡C— or a 3-5 membered saturated ring optionally containing one or more heteroatoms each independently selected from the group consisting of 0 and N, such —C.sub.1-4alkyl optionally substituted with OH;
R.sub.5 represents —C.sub.1-6alkyl, CFH.sub.2, CF.sub.2H, CF.sub.3, phenyl, pyridyl or a 3-7 membered saturated ring optionally containing one or more heteroatoms each independently selected from the group consisting of O, S, and N, such 3-7 membered saturated ring optionally being substituted with one or more substituents each independently selected from the group consisting of hydrogen, halogen, —C.sub.1-4alkyloxy, —C.sub.1-C.sub.4alkyloxycarbonyl, oxo, —C(═O)—C.sub.1-3 alkyl, —C.sub.1-4alkyl, OH, CN, CFH.sub.2, CF.sub.2H and CF.sub.3;
or a pharmaceutically acceptable salt or a solvate thereof.

Methods of Identifying Compounds

[0237] Also described herein are methods for identifying a compound useful for the treatment of infection by hepatitis B virus (HBV) and/or for clinically curing infection by HBV, comprising (a) measuring the ability of the compound to modulate core protein-mediated regulation of DNA (e.g., cccDNA) and (b) identifying the compound as useful for treating or clinically curing a hepatitis B infection based on the ability of the compound to modulate core protein-mediated regulation of DNA (e.g., cccDNA). The compound can modulate core protein-mediated regulation of DNA (e.g., cccDNA) by (a) modulating the structure of core protein; (b) modulating the function of core protein (thereby affecting, e.g., viral DNA levels, viral RNA levels, and/or viral antigen levels); (c) modulating the binding of core protein to DNA (e.g., cccDNA) (which can be assessed using, e.g., an EMSA assay); (d) depleting the amount of free core protein dimer available to bind to cccDNA; (e) altering nuclear import or export of core protein; (f) altering an interaction between DNA (e.g., cccDNA) and a chromatin component; (g) altering an interaction between core protein and a chromatin component; (h) altering the rate, quantity, quality or stability of RNA expressed from DNA (e.g., cccDNA); (i) altering the stability or maintenance of cccDNA; and/or (j) modulating an innate immune response against HBV. A compound useful for modulating an innate immune response against HBV can be assayed by measuring activation of APOBEC proteins (e.g., by PCT/sequencing of base pair changes secondary to APOBEC activity) or by assaying activation of a cytosolic DNA sensor, such as mitochondrial antiviral signaling protein (MAVS), DNA-dependent activator of IFN-regulatory factors (DAI), P202, LRRFIP1, or absent in melanoma 2 (AIM2), by evaluation of phosophostates and/or changes in downstream markers of immune activation.

[0238] The ability of the compound to modulate core protein-mediated regulation of DNA (e.g., cccDNA) can be measured by detecting a change in an amount of or state of core protein bound to DNA. Assays for detecting a change in the amount of core protein bound to DNA include chromatin immunoprecipitation (ChIP). Assays for detecting a change in the state of core protein bound to cccDNA include immunoprecipitation and mass spectrometry. A South-western blot of isolated DNA, e.g., cccDNA, can be performed using methods known in the art. Modulation of cccDNA can also be evaluated by a qPCR endpoint or real-time reporter assay in order to quantitatively assess either quantity of cccDNA compared to relaxed circular DNA (rcDNA), or to quantitatively assess production of viral RNAs. Other assays which can be used in the methods described herein include measuring viral antigen by ELISA and measuring viral RNA by qRT-PCR. Endpoint or real-time reporter assays can be used to detect changes in quantity of viral RNA (e.g., pgRNA) or protein production. Assays using energy transfer or quenching (1) between labeled core protein and DNA (e.g., cccDNA) or (2) between another DNA (e.g., cccDNA) binding protein and DNA can be used to show a compound's ability to, e.g., disrupt these binding interactions.

[0239] In some embodiments, the ability of the compound to modulate core protein-mediated regulation of DNA (e.g., cccDNA) also can be measured using assays known in the art to assess binding of the compound to a core protein dimer to determine whether the compound modulates core protein-DNA interaction (e.g., a core protein-cccDNA interaction). Differentially reporter-tagged core protein subunits can be used to assess binding of the compound to a core protein dimer. See, e.g., Example 3. In vitro binding of core protein to DNA (e.g., cccDNA) can also be measured. In one embodiment, binding is measure using a competition assay with control DNA. Methods of measuring the presence or quantity of a viral protein (e.g., HBsAg and HBeAg) or RNA (e.g., pgRNA) are known in the art. For example, clinical diagnostic kits for assessment of HBsAg and HBeAg are commercially available, e.g., from Roche® and Abbott®. In some embodiments, the ability of the compound to modulate core protein-mediated regulation of cccDNA is determined by differential scanning fluorimetry, isothermal calorimetry, thermopheresis, or Saturation Transfer Difference NMR. The method can also include varying the concentration of the compound until the compound modulates core protein-cccDNA interaction.

[0240] Methods for identifying a compound useful for the treatment of infection by hepatitis B virus (HBV) and/or for clinically curing infection by HBV, can include measuring the ability of the one or more compounds to modulate core protein structure or assembly and identifying the compound as useful for treating or clinically curing a hepatitis B infection based on the ability of the compound to modulate core protein. The method can also include measuring the ability of the compound to modulate core protein-mediated regulation of cccDNA.

[0241] Modulation of core protein activities can be measured by assessing binding of a labeled compound to a core protein dimer to determine whether the compound affects binding interactions of DNA (e.g., cccDNA) to the core protein dimer. Modulation of core protein assembly can also be determined by measuring fluorescence quenching of labeled core protein. See, e.g., Example 2. Modulation of core protein structure can be determined by measuring direct interaction with core protein, as with ITC or other methods known to those skilled in the art. The ability of the compound to modulate core protein can be measured by measuring altered binding of core protein to antibodies or other proteins sensitive to Cp tertiary or quaternary structure, immunoprecipitation and Western blot, sandwich ELISA, and/or a BRET assay.

Therapy, Administration and Dosage

[0242] The term “therapeutically effective amount” as used herein, refers to that amount of active compound or pharmaceutical agent that elicits the biological or medicinal response in a tissue system, animal or human that is being sought by a researcher, veterinarian, nurse, pharmacist, physician assistant, medical doctor or other medical provider, which includes alleviation of the symptoms of the disease or disorder being treated. In one aspect, the therapeutically effective amount is that which may treat or alleviate the disease or symptoms of the disease at a reasonable benefit/risk ratio applicable to any medical treatment. However, it is to be understood that the total daily usage of the compounds and compositions described herein may be decided by the medical provider within the scope of sound medical judgment. The specific therapeutically-effective dose level for any particular patient will depend upon a variety of factors, including the disorder being treated and the severity of the disorder; activity of the specific compound employed; the specific composition employed; the age, body weight, general health, gender and diet of the patient: the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidentally with the specific compound employed; and like factors well known to the researcher, veterinarian, nurse, pharmacist, physician assistant, medical doctor or other medical provider of ordinary skill.

[0243] It is also appreciated that the therapeutically effective amount, whether referring to monotherapy or combination therapy, is advantageously selected with reference to any toxicity, or other undesirable side effect, that might occur during administration of one or more of the compounds described herein. Further, it is appreciated that the co-therapies described herein may allow for the administration of lower doses of compounds that show such toxicity, or other undesirable side effect, where those lower doses are below thresholds of toxicity or lower in the therapeutic window than would otherwise be administered in the absence of a co-therapy.

[0244] As used herein, the term “composition” generally refers to any product comprising the specified ingredients in the specified amounts, as well as any product which results, directly or indirectly, from combinations of the specified ingredients in the specified amounts. It is to be understood that the compositions described herein may be prepared from isolated compounds described herein or from salts, solutions, hydrates, solvates, and other forms of the compounds described herein. It is also to be understood that the compositions may be prepared from various amorphous, non-amorphous, partially crystalline, crystalline, and/or other morphological forms of the compounds described herein. It is also to be understood that the compositions may be prepared from various hydrates and/or solvates of the compounds described herein. Accordingly, such pharmaceutical compositions that recite compounds described herein are to be understood to include each of, or any combination of, the various morphological forms and/or solvate or hydrate forms of the compounds described herein. Illustratively, compositions may include one or more carriers, diluents, and/or excipients. The compounds described herein, or compositions containing them, may be formulated in a therapeutically effective amount in any conventional dosage forms appropriate for the methods described herein. The compounds described herein, or compositions containing them, including such formulations, may be administered by a wide variety of conventional routes for the methods described herein, and in a wide variety of dosage formats, utilizing known procedures (see generally, Remington: The Science and Practice of Pharmacy, (21.sup.st ed., 2005)).

[0245] As used herein, the term “treatment” or “treating” means any administration of a compound or composition described and includes (1) inhibiting the disease in a patient that is experiencing or displaying the pathology or symptomatology of infection by HBV (i.e., arresting further development of the pathology and/or symptomatology), (2) ameliorating the disease in a patient that is experiencing or displaying the pathology or symptomatology of infection by HBV (i.e., reversing or lessening the pathology and/or symptomatology), inhibiting or (4) preventing of chronic infection by HBV. The term “controlling” includes preventing, treating, eradicating, ameliorating or otherwise reducing the severity of the infection by HBV, reducing production of new virions and/or prevention of hepatic inflammation.

[0246] As used herein, the term “curing” or “cure” means inactivation of cccDNA such that HBsAg and HBV DNA are produced at clinically insignificant levels. “Cure” or “curing” can also mean patients previously on therapy or requiring therapy are no longer deemed to require therapy for chronic HBV.

[0247] As used herein, the term “clinical outcome” means the manifestation of the disease in a patient that has experienced or displayed the pathology or symptomatology of infection by HBV after treatment. For example, a patient's clinical outcome can include inhibition or amelioration of the disease or symptoms of the disease or inhibiting or preventing of chronic infection or sequela of chronic infection by HBV.

[0248] The term “administering” as used herein includes all means of introducing the compounds and compositions described herein to the patient, including, but are not limited to, oral (po), intravenous (iv), intramuscular (im), subcutaneous (sc), transdermal, inhalation, and the like. The compounds and compositions described herein may be administered in unit dosage forms and/or formulations containing conventional nontoxic pharmaceutically-acceptable carriers, adjuvants, and vehicles.

[0249] Illustrative routes of oral administration include tablets, capsules, elixirs, syrups, and the like.

[0250] Illustrative routes for parenteral administration include intravenous, intraarterial, intraperitoneal, epidurial, intraurethral, intrasternal, intramuscular and subcutaneous, as well as any other art recognized route of parenteral administration.

[0251] Illustrative means of parenteral administration include needle (including microneedle) injectors, needle-free injectors and infusion techniques, as well as any other means of parenteral administration recognized in the art. Parenteral formulations are typically aqueous solutions which may contain excipients such as salts, carbohydrates and buffering agents (preferably at a pH in the range from about 3 to about 9), but, for some applications, they may be more suitably formulated as a sterile non-aqueous solution or as a dried form to be used in conjunction with a suitable vehicle such as sterile, pyrogen-free water. The preparation of parenteral formulations under sterile conditions, for example, by lyophilization, may readily be accomplished using standard pharmaceutical techniques well known to those skilled in the art. Parenteral administration of a compound is illustratively performed in the form of saline solutions or with the compound incorporated into liposomes. In cases where the compound in itself is not sufficiently soluble to be dissolved, a solubilizer such as ethanol can be applied.

[0252] The dosage of each compound of the claimed combinations depends on several factors, including: the administration method, the condition to be treated, the severity of the condition, whether the condition is to be treated or prevented, and the age, weight, and health of the person to be treated. Additionally, pharmacogenomic (the effect of genotype on the pharmacokinetic, pharmacodynamic or efficacy profile of a therapeutic) information about a particular patient may affect the dosage used.

[0253] It is to be understood that an effective amount of any one or a mixture of the compounds described herein can be readily determined by the attending diagnostician or physician by the use of known techniques and/or by observing results obtained under analogous circumstances. In determining the effective amount or dose, a number of factors are considered by the attending diagnostician or physician, including, but not limited to the species of mammal, including human, its size, age, and general health, the specific disease or disorder involved, the degree of or involvement or the severity of the disease or disorder, the response of the individual patient, the particular compound administered, the mode of administration, the bioavailability characteristics of the preparation administered, the dose regimen selected, the use of concomitant medication, and other relevant circumstances.

EXAMPLES

Example 1: Binding Labeled Compound to Cp or Capsid

[0254] A fluorescent CpAM, such as FL-HAP (see FIG. 2), is added to a sample of Cp149 capsid, Cp149 dimer, or a mixture of the two as might be found in an assembly reaction. The compound is allowed to equilibrate. The small molecule in the method is to resolve capsid, dimer and free small molecule by size exclusion chromatography where the amount of labeled compound and protein in each fraction can be measured (see FIG. 2). (ii) Another method is measure changes in the fluorescence anisotropy of the small molecule when it binds to the much larger protein or protein complex. (iii) Yet another method is to look for changes in the fluorescence emission associated with the environment of the binding site compared to bulk solution. Another method to detect binding is to observe differences between free and bound fluorophore to the effect of a collisional quencher, such as iodide and acrylamide.

Example 2: Measuring Assembly as a Means for Monitoring Depletion of Free Dimer

[0255] A high throughput fluorescence based assay is used to measure assembly as a means for monitoring depletion of free dimer. As shown in FIG. 3A, dimers have cysteines engineered at the C-termini, at either end, which is labeled with BoDIPY-FL, Cp150Bo. The free dimer is highly fluorescent. When Cp is induced to assemble by adjusting buffer conditions, as modulated by CpAMs, the fluorescent moieties are brought into close proximity at fivefold and quasi-sixfold vertices resulting in self-quenching of fluorescence. As shown in FIG. 3B, dimers are fluorescent (left) capsids (right) are not. FIG. 3C shows that the change in fluorescence matches other methods of monitoring assembly, such as light scattering (LS). Fluorescence monitoring has been used extensively to find molecules that drive assembly. Here the goal is to deplete the concentration of free Cp.

Example 3: Assays Employing Differentially Reporter-Tagged Core Protein Subunits; In Vitro Binding of Core Protein to cccDNA; and In Vitro Binding of Labeled or Unlabeled Core Protein to Labeled or Unlabeled cccDNA in Competition with Unlabeled or Labeled Control DNA

[0256] In one form, unlabeled Cp is added to unlabeled HBV DNA and binding assessed by gel shift. Addition of a CpAM can differentially increase or weaken binding to viral DNA.

[0257] In a second form, Cp, fluorescently labeled, for example, by modification of cysteine 183 is used to titrate HBV DNA and the complex observed by native agarose gel shift.

[0258] In a third variation, the viral DNA is amplified by PCR using fluorescent oligomers. Binding to the labeled oligomer, especially in competition with non-specific unlabeled DNA can be measured by gel shift in a native agarose gel. CpAM addition will modify binding to labeled DNA.

[0259] Cp binding to HBV DNA and non-specific competitor DNA also can be measured in a high throughput manner. The HBV DNA is unlabeled and may be linear, as a component in a circular relaxed plasmid, or as a component on a circular supercoiled plasmid. The competitor DNA is an oligomer of 15 to 30 nucleotides whose sequence is only limited in that it not include specific HBV binding sites whether natural or synthetic. In this assay the Cp is labeled with a fluorophore, typically fluorescein. Also, a mutant Cp that carries the assembly-preventing Y132A mutant is used. There are two methods of read out. In the first method binding to cccDNA (1 MDa DNA or part of >2 MDa plasmid) is read by observing the anisotropy of the labeled Cp so that CpAM-induced loss of specificity is observed as a decrease in anisotropy. In the second method, the competitor DNA oligomers are labeled with a quencher so that CpAM-induced loss of specificity is read as a decrease in fluorescence.

Example 4: Binding Assays Using Fluorescence

[0260] In another example, a fluorescently-labeled a structurally sensitive fluorophore is appended to a Cp-binding CpAM. The compound will exhibit enhanced fluorescence when bound to Cp conformations and substrates of interest. The bound compound can be displaced by a molecule competing for its ligand binding site or by a molecule binding to a different site that allosterically disrupts normal interactions at its binding site.

Example 5: Size Exclusion Chromatography (SEC) and Anisotropy

[0261] SEC can read out hydrodynamic radius (Stokes' radius) of a molecule as can anisotropy. Mutations of Cp that affect its in vitro activity (e.g. assembly) can appear as structural effects evident in SEC. Thus, simply measuring changes in SEC elution or change in anisotropy of a C-terminally labeled Cp signals both binding of Cp by a CpAM and a change in Cp structure. An exemplary SEC experiment is shown in FIG. 4.

Example 6: Differential Scanning Fluorimetry (DSF)

[0262] Differential scanning fluorimetry (DSF) measures the temperature dependence of a protein's interaction with a fluorescent dye. Small molecules that bind to the target protein affect its stability. Using Sypro orange as the reporter dye we observe that HBV dimer undergoes two distinct dye-binding transitions at 66° C. and 74° C. Some dimer binding molecules have substantial concentration-dependent effects on the amplitude of the first transition. The concentration dependence provides a 66° C. KD value. The percent of the maximal amplitude change is reported as % ΔF. A few molecules also affect the second transition. These interactions demonstrate a drug-dependent change in the Cp-melting transition that correlates with limiting the structural transitions available to Cp.

Example 7: Microscale Thermophoresis

[0263] Microscale thermophoresis measures binding of macromolecules (i.e. a protein) by relating binding to changes in diffusion of fluorescently-labeled molecule through a laser induced micro-temperature gradient. Moderate throughput of very small volume samples can be achieved. The instrument is sensitive to conformational changes and can thus be used with fluorescently labeled protein when the ligand modulates Stokes' radius. Thus the effects demonstrated in the SEC experiments will be readily evaluated. Of course, binding of a fluorescently-labeled small molecule to Cp 149 can easily be evaluated. Its displacement by unlabeled analogs that bind a competitive site is used to measure a dissociation constant.

Example 8: Saturation Transfer Difference NMR (STD-NMR)

[0264] Saturation Transfer Difference NMR (STD-NMR) is a method for detecting ligands that are transiently bound to a protein. The protein resonances are saturated so that energy will be transferred to bound ligand, affecting the ligand NMR spectrum and allowing identification of parts of the ligand that are in contact with its receptor. Using solution conditions that inhibit assembly, ligand-Cp dimer interactions can be investigated.

Example 9: Cell Based Assays

[0265] 96-well plates (coated/white) are seeded with HBV producing cells in growth medium and incubated at 37° C. overnight. Test compound placed into wells as a single dose or as a series of dilutions. Media is replaced and new compounds added every 3 days. On day 9, cell viability/compound toxicity is measured and supernatant is assayed for viral load (genome equivalents) by qPCR, HBsAg and HBeAg (e.g., by ELISA).

[0266] This screening assay can also be performed with only one treatment day (in contrast to two treatment days as described above).

EQUIVALENTS

[0267] Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.

INCORPORATION BY REFERENCE

[0268] The entire contents of all patents, published patent applications, websites, and other references cited herein are hereby expressly incorporated herein in their entireties by reference.