Regulator for increasing plant beta-glucan content

Abstract

A regulator for increasing plant β-glucan content includes the following components by weight: 30-50 parts of a magnetic material, 20-30 parts of an oyster shell powder, 20-30 parts of an illite powder, and 10-20 parts of a mushroom polysaccharide extract.

Claims

1. A regulator for increasing plant β-glucan content, consisting of the following components by weight: 30-50 parts of a magnetic material, 20-30 parts of an oyster shell powder, 20-30 parts of an illite powder, and 10-20 parts of a mushroom extract, wherein the magnetic material is a mixture of a permanent ferrite powder and a magnetite powder, and the magnetite powder is Panzhihua vanadium-titanium magnetite; wherein the mushroom extract is a shiitake mushroom extract, a straw mushroom extract, a signorina mushroom extract, a boletus extract, or a mixture thereof; and wherein the mushroom extract is prepared by the following steps: freeze drying a raw material, crushing the raw material to 30 mesh at room temperature, adding water in an amount of 5-10 times the weight of the raw material, adding calcium hydroxide in an amount of 0.1% of the water, stirring, conducting an ultrasonication extraction for 2-3 hours, filtering to obtain a filtrate, concentrating the filtrate under reduced pressure, and freeze drying to obtain the mushroom extract.

2. A regulator for increasing plant β-glucan content, consisting of the following components by weight: 39 parts of a magnetic material, 28 parts of an oyster shell powder, 21 parts of an illite powder, and 12 parts of a mushroom extract.

Description

DETAILED DESCRIPTION OF THE ILLUSTRATED EMBODIMENTS

(1) Reference will now be made in detail to embodiments of the present invention.

EXAMPLE 1

(2) A regulator for increasing plant β-glucan content includes the following components by weight: 33 parts of a magnetic material, 26 parts of an oyster shell powder, 25 parts of an illite powder, and 16 parts of a mushroom polysaccharide extract.

(3) Among them, the magnetic material is a mixture of a permanent ferrite powder (purchased from Dongyang Hengdian Excellent Magnet Factory) and Panzhihua vanadium-titanium magnetite powder (from the Panzhihua vanadium-titanium magnetite deposit 15 km northeast of Panzhihua City, crushed to 50 mesh). The mass ratio of the permanent ferrite powder to the Panzhihua vanadium-titanium magnetite powder is 35:65.

(4) The mushroom polysaccharide extract is prepared as follows: taking the freeze-dried raw material (shiitake mushroom and boletus, 1:1 by weight), pulverizing it to 30 mesh at room temperature, adding water in an amount of 10 times the weight of the raw material, and adding calcium hydroxide in an amount of about 0.1% of the water mass, mixing well, conducting an ultrasonication extraction for 3 hours, filtering, distilling under reduced pressure, and freeze-drying to obtain the extract as a powder.

(5) The above materials are weighed and mixed uniformly to obtain “Regulator A.”

EXAMPLE 2

(6) A regulator for increasing plant β-glucan content includes the following components by weight: 39 parts of a magnetic material, 28 parts of an oyster shell powder, 21 parts of an illite powder, and 12 parts of a mushroom polysaccharide extract.

(7) Among them, the magnetic material is a mixture of a permanent ferrite powder (purchased from Dongyang Hengdian Excellent Magnet Factory) and Panzhihua vanadium-titanium magnetite powder (from the Panzhihua vanadium-titanium magnetite deposit 15 km northeast of Panzhihua City, crushed to 50 mesh). The mass ratio of the permanent ferrite powder to the Panzhihua vanadium-titanium magnetite powder is 35:65.

(8) The mushroom polysaccharide extract is prepared as follows: taking the freeze-dried raw material (shiitake mushroom and boletus, 1:1 by weight), pulverizing it to 30 mesh at room temperature, adding water in an amount of 10 times the weight of the raw material, and adding calcium hydroxide in an amount of about 0.1% of the water mass, mixing well, conducting an ultrasonication extraction for 3 hours, filtering, distilling under reduced pressure, and freeze-drying to obtain the extract as a powder.

(9) The above materials are weighed and mixed uniformly to obtain “Regulator B.”

EXAMPLE 3

(10) A regulator for increasing plant β-glucan content includes the following components by weight: 30 parts of a magnetic material, 30 parts of an oyster shell powder, 30 parts of an illite powder, and 10 parts of a mushroom polysaccharide extract.

(11) Among them, the magnetic material is a mixture of a permanent ferrite powder (purchased from Dongyang Hengdian Excellent Magnet Factory) and Panzhihua vanadium-titanium magnetite powder (from the Panzhihua vanadium-titanium magnetite deposit 15 km northeast of Panzhihua City, crushed to 50 mesh). The mass ratio of the permanent ferrite powder to the Panzhihua vanadium-titanium magnetite powder is 35:65.

(12) The mushroom polysaccharide extract is prepared as follows: taking the freeze-dried raw material (shiitake mushroom and boletus, 1:1 by weight), pulverizing it to 30 mesh at room temperature, adding water in an amount of 10 times the weight of the raw material, and adding calcium hydroxide in an amount of about 0.1% of the water mass, mixing well, conducting an ultrasonication extraction for 3 hours, filtering, distilling under reduced pressure, and freeze-drying to obtain the extract as a powder.

(13) The above materials are weighed and mixed uniformly to obtain “Regulator C.”

EXAMPLE 4

(14) This example is a comparative example. Compared with Example 2, this example does not include the oyster shell powder, i.e. the materials include 39 parts of the magnetic material, 21 parts of the illite powder, and 12 parts of the mushroom polysaccharide extract. The rest is the same as in Example 2. “Comparative Example A” is obtained.

EXAMPLE 5

(15) This example is a comparative example. Compared with Example 2, this example does not include the illite powder, that is, materials include 39 parts of the magnetic material, 28 parts of the oyster shell powder, and 12 parts of the mushroom polysaccharide extract. The rest is the same as in Example 2. “Comparative Example B” is obtained.

EXAMPLE 6

(16) This example is a comparative example. Compared with Example 2, this example does not include the magnetic material, i.e. the materials include 28 parts of oyster shell powder, 21 parts of the illite powder, and 12 parts of the mushroom polysaccharide extract. The rest is the same as in Example 2. “Comparative Example C” is obtained.

EXAMPLE 7

(17) This example is a comparative example. Compared with Example 2, this example does not include the mushroom polysaccharide extract, i.e. the materials include 39 parts of the magnetic material, 28 parts of the oyster shell powder, and 21 parts of the illite powder. The rest is the same as in Example 2. “Comparative Example D” is obtained.

EXAMPLE 8

(18) In this example, planting rice was used as an example, and a test was performed in a paddy field in Pengzhou, Sichuan, China. Base fertilizer, tiller fertilizer, and ear fertilizer (including combined fertilizers including nitrogen, phosphorus, potassium etc.) were applied according to conventional methods, respectively. Parallel experiments were carried out on six paddy fields with an area of about 1 mu each. In five of the test fields, 9% of “Regulator B,” “Comparative Example A,” “Comparative Example B,” “Comparative Example C,” and “Comparative Example D,” were added to the fertilizers applied each time. For the remaining paddy field, no regulator was added, proceed as usual, i.e., “Blank Example.”

(19) After harvesting, the rice was dried, and the β-glucan in the rice was measured. The contents of β-1,3 glucan and β-1,6 glucan in the total β-glucan were measured, respectively.

(20) The specific measuring method is:

(21) The sample to be tested was first crushed and passed through a 35-mesh sieve. 2 g of the sample was placed in a 100 mL centrifuge tube, 10 mL of ethanol-water solution (50%, v/v) and 30 mL of sodium phosphate buffer solution (20 mM, pH 6.5) were added to the centrifuge tube. The centrifuge tube was heated in boiling water for 5 minutes, shaken, and then heated again for 3 minutes. Gel pieces were not formed in the centrifuge tube. After the centrifuge tube was cooled to 40° C., 2 mL of lichenase was added. After enzymolysis at 40° C. for 1 hour, 18 mL of water was added to adjust the volume to 60 mL. Then it was centrifuged at 1000 r/min for 10 min, and 1 mL of the supernatant was transferred to a glass test tube. 1 mL of β-glucanase was added and hydrolyze at 40° C. for 15 min. 3 mL of GOPOD reagent was added and reacted at 40° C. for 20 min. The absorbance at 510 nm was measured, and converted to the percentage content. The results are shown in Table 1.

(22) TABLE-US-00001 TABLE 1 Contents of Total β-glucan in Rice Obtained by Various Treatment Methods (%) Regulator Comparative Comparative Comparative Comparative Blank Groups B Ex. A Ex. B Ex. C Ex. D Example Content 5.61 4.93 5.35 2.36 1.98 0.39

(23) In addition, in terms of the amount of pesticide used, the amount used for the Regulator B group was reduced by about 20% compared with the Blank Example.

(24) The β-1,3 glucan content was measured using a β-1,3 glucanase detection kit from Solarbio. The results are shown in Table 2.

(25) TABLE-US-00002 TABLE 2 Contents of β-1,3 Glucan in Rice Obtained by Various Treatment Methods (%) Regulator Comparative Comparative Comparative Comparative Blank Groups B Ex. A Ex. B Ex. C Ex. D Example Content 5.33 4.68 5.11 2.01 1.73 0.35

EXAMPLE 9

(26) In this example, planting tomato was used as an example. Tomato was planted in the way as Example 8 except that the planting type, fertilization period, and the amount of fertilizer used were different. The method for measuring β-glucan is also the same as that in Example 8. The results are shown in Table 3.

(27) TABLE-US-00003 TABLE 3 Total β-Glucan Content in Tomatoes Obtained by Various Treatment Methods (%) Regulator Comparative Comparative Comparative Comparative Blank Groups B Ex. A Ex. B Ex. C Ex. D Example Content 2.69 2.31 2.39 0.96 0.93 0.31