Dual purpose pollenizer watermelons

Abstract

The application relates to the field of plant breeding, in particular watermelon breeding. Provided are diploid watermelon plants (and seeds from which these plants can be grown) which produce small, diploid, red watermelon fruits. Also provided are small, diploid watermelon fruits having an average weight of less than 1.8 kg.

Claims

1. A seed of a watermelon variety designated WH9716, a representative sample of seeds of WH9716 having been deposited under Accession Number NCIMB 42704.

2. A plant, or a part thereof, produced by growing the seed of claim 1.

3. The plant or part thereof of claim 2, produced by in vitro culture or by grafting of a scion derived from WH9716 to a different rootstock, a representative sample of seeds of WH9716 having been deposited under Accession Number NCIMB 42704.

4. A fruit produced on the plant of claim 2.

5. The fruit of claim 4, wherein said fruit has an average rind thickness on the sides of at least 0.2 cm and at most 0.4 cm.

6. A watermelon plant which is regenerated from the plant part of claim 2, wherein the part is a scion, a cutting, a rootstock, a cell, a cotyledon, a fruit, a shoot, a shoot tip, a hypocotyl, an embryo, a seed, a protoplast, a leaf, a meristem, a flower or a stem, a part of any of these, or any combination thereof, and wherein the regenerated plant has all the physiological and morphological characteristics of variety WH9716 when grown under the same conditions, wherein a representative sample of seeds of variety WH9716 has been deposited under accession number NCIMB 42704.

7. A fruit produced on the plant of claim 6.

8. The fruit of claim 7, wherein said fruit has an average rind thickness on the sides of at least 0.2 cm and at most 0.4 cm.

9. The seed of claim 1, wherein the seed is primed and/or wherein the seed further comprises one or more of: a biological control agent, a fungicidal composition, a insecticidal composition, a nematicidal composition, an acaricidal composition, or any combination thereof.

10. The plant of claim 2, wherein the plant comprises a different rootstock.

11. The plant of claim 6, wherein the plant comprises a different rootstock.

12. A method for producing triploid watermelon fruits a field, comprising: a) interplanting a triploid watermelon plant and the plant of claim 2 in a field; b) allowing pollen from the plant of claim 2 to pollinate flowers of the triploid plant to produce triploid hybrid plants; and c) harvesting fruits produced on the triploid hybrid plants.

13. A method for producing triploid watermelon fruits a field, comprising: a) interplanting a triploid watermelon plant and the plant of claim 6 in a field; b) allowing pollen from the plant of claim 6 to pollinate flowers of the triploid plant to produce triploid hybrid plants; and c) harvesting fruits produced on the triploid hybrid plants.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 shows fruits of exemplary pollenizers according to the invention and described in Example 2.

(2) FIG. 2 shows exemplary pollenizers according to the invention and described in Example 5.

DETAILED DESCRIPTION OF EMBODIMENTS OF THE INVENTION

(3) The invention provides plants (and seeds from which such plants are grown) of the species Citrullus lanatus, wherein said plant is suitable as a pollenizer in triploid watermelon production, is diploid and produces marketable diploid fruits having an average weight of less than 2.0 kg, preferably less than 1.8 kg, more preferably equal to or less than 1.7, 1.6, 1.5, 1.4, 1.3, 1.2, 1.0 kg, more preferably equal to or less than 0.9, 0.8, 0.7, 0.65 kg, such as equal to or less than 0.6, 0.5, 0.4, 0.35, 0.3 or 0.25 kg at harvest (i.e. at maturity). In a further embodiment average fruit size is equal to or less than about 0.9 kg (such as 0.8, 0.7, 0.65, 0.6, 0.5, 0.4 kg) but above 0.25 kg. To determine average fruit characteristics, such as average fruit weight, of a plant line or hybrid according to the invention several plants of a line or hybrid are grown in one location and 3, 4, 5 or more fruits are harvested from 2, 3, or more plants of the same line and e.g. weighed. Reference plant lines (such as known pollenizers) can be grown at the same location (e.g. in the same trial or under the same environmental conditions) as a comparison. The average fruit weight according to the invention is in one embodiment preferably less than 0.65 kg, more preferably equal to or less than about 0.6, 0.5, 0.4, 0.3 or 0.25 kg. In another embodiment the average fruit size is less than 0.65 kg but above 0.25 kg.

(4) Fruit diameters can be variable, but preferably average fruit length is less than 12 cm, more preferably equal to or less than about 11.5 or 11 cm, such as equal to or less than 10.5, 10.0, 9.5, 9.0, 8.5 or 8.0 cm, while the average fruit width is preferably less than 11, 10.5 or 10 cm, such as equal to or less than 9.5, 9.0, 8.5, 8.0 cm. Thus, average fruit dimensions range have ranges of 8.0-12.0 cm average length×8.0-11 cm average width. Preferably at least fruit length is on average below 12 cm, preferably below 11.5 cm, more preferably about 11 cm or less. Preferably, both length and width of the fruits is on average about 11 cm or less. In one embodiment the average length by width is 10×11 cm, or less. In one embodiment average fruit dimensions for both lengths and width are equal to or below 10.5 cm. These dimensions are particularly encompassed for the diploid fruit weights of less than 0.65 kg on average. For the heavier diploid fruits (e.g. less than 1.8 kg but above 0.65 kg) also larger dimensions are encompassed herein, such as an average length of equal to or less than 17 cm (such as 16, 15, 14, 13, 12 cm) and an average width of equal to or less than 15 cm, such as 14, 13, 12 cm).

(5) The fruits, i.e. the fruit flesh, produced on the pollenizer plants are edible at maturity. The flesh color of the mature fruits of the diploid plants according to the invention is in one embodiment red, having an RHS mini color chart value (Royal Horticultural Society mini color chart) of 39 or higher, especially between 39 and 41. Especially fruits with flesh colors RHS 39B, 41A, 41B, 42 A, 42B, 43A, 43B, 44A, 44B are encompassed. Also, the average percent Total Soluble Solids (% TTS; herein also referred to as degrees Brix, or ° Brix) of the fruits is at maturity at least about 7.5%, preferably at least about 8% or 8.5%, more preferably at least about 9% or 9.5%, and even more preferably at least about 10%, 10.5%, 11%, 11.5%, 12%, 12.5%, 13%, or more. Average TSS can, for example, be determined using a refractometer as described in the Examples. The average percentage TSS of the diploid fruits produced by the pollenizer plants according to the invention can be increased by traditional breeding techniques, e.g. by crossing the plants provided herein with watermelon plants comprising high TSS and selection of progeny (e.g. obtainable by one or more selfings and/or backcross populations) producing fruits with higher TSS values while maintaining small fruit size.

(6) As mentioned, the diploid fruits according to the invention are edible, i.e. they have fruit quality characteristics which make them marketable for human consumption. This means that the fruits have good flavor properties (no off-flavors etc.). For fruits to have at least good flavor properties a minimum average brix of at least about 7.5 degrees is desired. Flavor properties can be determined and scored (e.g. as bad, good, very good) by trained test-panels using known methods for evaluating sensory properties of fruits (Karen L. Bett, Ch 13, Fresh-Cut Fruits and Vegetables, Science, Technology, and Market; Edited by Olusola Lamikanra, CRC Press 2002, Print ISBN: 978-1-58716-030-1). Selection for good flavor includes test panels to select against bitterness and other unpleasant flavors, such as caramel flavor. Watermelon checks of varieties which have good flavor properties (e.g. Allsweet, Crimson Sweet) are preferably included in the test.

(7) The fruits should preferably also not be susceptible to what is known as “fruit cracking” and/or should preferably not contain the brittle gene as present in SP-1. The Super Pollenizers such as SP-1 (as described in WO03/075641) bear brittle fruits, which makes the fruits (in particular the fruit flesh) unmarketable as fresh produce (although the seeds contained within the fruits can be harvested and marketed). Crack-resistance is generally selected for during breeding (e.g. in field observations and/or using for example pressure tests or other tests), as cracking is an undesired fruit quality characteristic. See also Haikun et al. 2010, Acta Hort. (ISHS) 871:223-230).

(8) Other fruit characteristics can be introduced by traditional breeding methods (see further below) and thereby combined with pollenizers producing small, edible diploid fruits according to the invention. For example plants can be selected which produce small, edible fruits, as described above, with increased or reduced rind thickness, increased or reduced rind brittleness, various skin/rind colors (e.g. light green; dark green; green-striped with narrow, medium or wide stripes; grey types; with or without spotting; Golden yellow) and rind surfaces (e.g. furrowed or smooth surface), flesh structure/flesh firmness, different fruit shapes (elongate, oval, blocky, spherical or round), higher brix content, higher lycopene and/or vitamin content, different sugar:acid ratios, very good fruit flavor, etc. Also the combination of small edible fruits with another flesh color than red is possible, for example genetic determinants for yellow flesh or orange flesh or white flesh may be introduced, e.g. by backcross breeding with another color-type. See Guner and Wehner 2004, Hort Science 39(6): 1175-1182, in particular pages 1180-1181 describing genes for fruit characteristics. Generally important breeding objectives are early maturity, high fruit yield, high internal fruit quality (good uniform color, high sugar, proper sugar:acid ratio, good flavor, high vitamin and lycopene content, firm flesh texture, non-fibrous flesh texture, freedom from defects such as hollow heart, rind necrosis, blossom-end rot or cross stitch and good rind characteristics and cracking-resistance).

(9) Rind thickness is a characteristic which influences damage during handling and transporting (too thin or too brittle rind), but thin rinds may also be desirable for consumers. For example, when whole fruits are eaten like apples, a thin rind can be desirable. In one embodiment the fruits have a thin rind, such as an average rind thickness (measured on the side) of at least about 0.2 cm, 0.21 cm, 0.22 cm, 0.23 cm, 0.24 cm, 0.25 cm, 0.26 cm, 0.27 cm, 0.28 cm, 0.29 cm, 0.3 cm, 0.4 cm, but less than 0.5 cm, more preferably less than or equal to 0.4 cm, such as 0.2 cm to 0.4 cm. In a preferred aspect the rind is at least 0.2 cm and at most 0.3 cm, preferably between about 0.2 and 0.3 cm. Thus, in one embodiment the rind is thicker than the rind of SP-4 fruit, but thinner than the rind of Polimax, and optionally thinner than the rind of Sidekick fruit. For certain embodiments thicker rinds may be desired and small fruits having a rind of 0.5 or more cm, such as 0.6 and 0.7 cm are also encompassed herein. The rind of the fruits preferably does not crack easily (i.e. is cracking-resistant), both in fruits with thin rinds and thick rinds.

(10) In one embodiment of the invention, the fruits preferably also do not have a brittle rind and/or an explosive rind as described in WO03/075641 on page 13 and 14, i.e. the fruits do not break under pressure in the range of 90 to 140 g/mm.sup.2.

(11) Flesh firmness of the diploid fruits is preferably at least about 0.8 (average firmness in kg as in Example 1), more preferably at least about 0.9, 1.0, or more. Flesh firmness can be increased by e.g. crossing with watermelons having firmer fruit flesh and selection for firmer flesh without increasing fruit size. Plants producing fruits with ultra-firm flesh are, for example, described in US2006/0005284.

(12) The diploid plants provided herein are suitable as pollenizers, which means that they produce sufficient pollen at the right time of the day and for an appropriate period of time to induce fruit set in triploid hybrids, leading to a (average) triploid fruit yield at least comparable to that obtained when using e.g. Polimax as pollinator. However, the plants according to the invention need not be sold or marketed as pollenizer for triploid fruit production and need not be used as pollenizer in triploid fruit production. They may also be marketed and/or used solely for diploid fruit production on their own.

(13) In one embodiment the pollenizer plants preferably produce a large number of male flowers at the appropriate time during flowering of the triploid hybrids, preferably at least about 35 open male flowers at day 15 and/or at least about 30 open male flowers at day 22 from the first day of flowering, although the number of male flowers is not critical in determining triploid fruit set, as long as sufficient pollen is produced by the available male flowers to lead to good triploid fruit set. As the pollenizer plants are also used for diploid fruit production, also sufficient female flowers must be produced by the pollenizers to ensure their dual purpose.

(14) As can be seen in the Examples (e.g., Example 2, Table 4), many open male and female flowers were present over the 3 week period counted. At the flowering date (the date when more than 50% of the plant in the plot has male/female flowers) the 11 pollenizer lines had on average 12.6 open male and 3.9 open female flowers. Each line generally had significantly more open male flowers than SP-4 and Polimax. At day 15 and 22 from 1st day of flowering all lines produce significantly more open male flowers than Sidekick, SP-4 and Polimax, all having at least 35 open male flowers at day 15 and at least 30 open male flowers at day 22. However, Polimax is a very good pollenizer for triploid hybrids, despite the fact that the number of male flowers is significantly lower than in the hybrids provided in the Examples. Polimax had only about 18 open male flowers at day 15 and day 20. The pollenizers according to the invention, therefore, may have a lower number of male flowers than in Examples (e.g., Example 2, Table 4), e.g. plants having about the same number of open male flowers as Polimax are encompassed herein, i.e. pollenizers having at least about 15, 18, 20, 25, 30, or more open male flowers at day 15 and/or at day 22 from the 1st day of flowering are also an embodiment of the invention.

(15) Also the number of open female flowers was good, indicating that the pollinizers are indeed suitable for dual-purposes, i.e. pollination and fruit production of triploid hybrids and/or pollination and fruit production on the diploid plants themselves (fruits produced by self-pollination of the pollenizer plant).

(16) In one embodiment of the invention the pollenizer plants are dual purpose pollenizer plants. In particular, the average number of open male flowers at day 22 from flowering is at least 15, 18, 20, 25 or 30 or more.

(17) In one embodiment the pollenizer plants according to the invention are hybrid diploids (F1 diploids) and not open pollinated (OP).

(18) In yet a further embodiment of the invention, the dual purpose pollenizer plants are not of the “edible seed watermelon” or “confectionary” type, as for example produced in variety San Juan (Native Seeds). These types of watermelon are produced to harvest the seeds for consumption or seed oil production and the fruits produce large, black or red edible seeds with soft seed coats, see e.g. Zhang 1996, Cucurbit Genetics Cooperative Report 19:66-67 (article 24) and Zhang and Wang, 1990, Cucurbit Genetics Cooperative Report 13:43-44 (article 16). In contrast, the seeds produced in the diploid fruits according to the invention are small (preferably tomato seed size to medium seed size, but not large) and are not suitable for seed harvest and seed consumption. In one embodiment of the invention the seeds of the diploid fruits are on average shorter than or equal to 5 mm in length, for example shorter than or equal to 4 mm, 3.5 mm, 3 mm, 2.5 mm, 2 mm, 1.5 mm or 1 mm in length.

(19) The dual purpose pollenizer plants not only produce very small, edible fruits, but also high numbers of marketable fruits. In one embodiment a plant according to the invention produces at maturity on average at least about 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 or more fruits per plant, more preferably at least 25 fruits, more preferably at least about 27, 28, 29, 30, 35, 40, 50, 60, 65 or more fruits.

(20) The pollenizer plants according to the invention can be combined with different vine types, such as compact vine type, standard vine type or, most preferably an “intermediate” vine type using normal breeding techniques. For example the pollenizers according to the invention may be crossed with a standard vine type and progeny may be selected which have an intermediate vine type.

(21) It is understood that it is also an object of the invention to provide seeds from which the pollenizer plants described herein can be grown. Also seedlings, scions and rootstocks, as well as cells and tissues of the pollenizer plants are encompassed herein. Such plant parts comprise the genetic determinants for producing dual purpose pollenizer plants according to the invention. Thus whole plants obtained from seedlings, scions and rootstocks, as well as cells and tissues of the pollenizer plants retain all the physiological and morphological characteristics of the pollenizer plants according to the invention when grown under the same environmental conditions.

(22) It is a further object of the invention to provide a plurality of diploid watermelon fruits obtainable on a pollenizer plant according to the invention as described above, and/or seeds present in those fruits. Thus, in one embodiment, harvested diploid fruits are provided, such as packaged whole fruits or fruit parts and/or processed fruits or fruit parts.

(23) Also progeny of the plants according to the invention are provided herein, such as seeds obtainable by crossing a pollenizer plant described herein with another watermelon plant and/or by selling a plant according to the invention to produce F1 seeds (and F1 plants grown from these seeds, as well as fruit produced by self-pollinating the F1 plants).

(24) Further provided are plant cells, cell cultures or tissue cultures of plants according to the invention, as well as root stocks, scions, transplants and vegetative propagations of plants according to the invention or of progeny thereof.

(25) In one aspect, a representative sample of seeds of the plants according to the invention are deposited under accession number NCIMB 41773 (WH9307) or accession number 42704 (WH9716).

(26) In one aspect a watermelon plant (or a seed from which the plant can be grown), or fruit and/or seed thereof, or part of the plant, having essentially all morphological and physiological fruit characteristics and/or flowering characteristics of the plants of which representative samples of seeds have been deposited are provided.

(27) In another aspect a watermelon plant (or a seed from which the plant can be grown), or fruit and/or seed thereof, or part of the plant, having essentially all morphological and physiological characteristics of the plants of which representative samples of seeds have been deposited are provided.

(28) Plants having the genetic determinants for producing small, edible fruits are, therefore, obtainable from the deposited seeds. The genetic determinants (i.e. the combination of genes) for producing small, edible fruits can be transferred to other watermelon plants, for example to create other diploid pollenizers with this phenotype or other diploids (open pollinated or inbred lines, or hybrid diploids). This can be done by using the pollenizers according to the invention as a parental line in breeding methods, i.e. as male or female parent in a cross with another watermelon plant. Known breeding methods can be used alone or in combination, such as (but not limited to) recurrent selection, pedigree breeding, backcross breeding, inbred development, hybrid testing, marker assisted breeding, etc. Diploids may also be used for tetraploid development, using e.g. colchicine treatment. Progeny are then selected which retain the small fruit dimensions and fruit weight, a brix of at least 7.5% and dual purpose pollenizer characteristics, all as described herein.

(29) Other watermelon plants may be used as a starting point to develop dual purpose pollenizer plants producing small, edible fruits according to the invention. For example, small fruited cultivars or lines may be used as starting material, such as for example Sidekick (U.S. Pat. No. 7,314,979) and/or SP-1 (WO 03/075641) and/or watermelon plants carrying the “tomato seed” mutant (gene ts) described in Guner and Wehner (2004, HortScience 39(6):1175-1182) and obtainable from gene curator of the Cucurbit Genetics Cooperative), and selecting for fruit quality characteristics (e.g. high brix, good flavor), small fruit size (e.g. small fruit dimensions and e.g. less than 0.9 kg weight) and small seed size in the diploid fruits, as well as pollenizer characteristics (e.g. many male flowers) as described herein.

(30) Selection for small seed size encompasses selecting for an average seed length of equal to or less than 8 mm, preferably 7 mm, 6 mm or more preferably equal to or less than 5.0 mm, preferably equal to or less than 4.5 mm, e.g. equal to or less than about 4.0 mm, 3.5 mm, 3.0 mm, 2.5 mm or 1.5 mm or 1.0 mm seed length.

(31) In one embodiment a breeding method for producing diploid dual purpose pollenizers according to the invention is provided, comprising: a) providing a breeding population of diploid watermelon plants, and b) selecting progeny for small fruit size, small seed size, high brix, good flesh color and pollenizer characteristics (all as described throughout this application).

(32) Plants obtainable by this method are encompassed herein. As mentioned, the breeding population can be provided by using at least one, preferably two small-fruited parents and crossing these, to generate an F1 and further progeny generations (F2, etc.). For example, one of the plants provided herein (e.g. WH9307) is used in step a) and is crossed to another watermelon plant. The progeny generations are then selected for at least the characteristics described in b).

(33) In one embodiment one or more hybrid plants is provided, obtained from crossing a pollenizer plant according to the invention with another watermelon plant and harvesting the F1 seeds of said cross. The F1 seeds may then be grown into F1 plants and self-pollinated or sib-pollinated to produce F2 seeds. If the parents used in the initial cross differ by one or more characteristics (e.g. disease resistance and fruit size), the F2 population will segregate for these trait(s) and the breeder can select plants in this and/or further progeny generations (F3, F4, etc.) which combine the desired traits (e.g. small fruit size and disease resistance). Alternatively, the F1 may be backcrossed to the recurrent parent (e.g. the pollenizer according to the invention into which a trait is to be introduced) or the F1 may be selfed to produce an F2 population segregating for the trait of interest and selected F2 plants having the trait of interest may be backcrossed to the recurrent parent.

(34) Thus, one or more traits not present in the pollenizer plants according to the invention can be introduced into a pollenizer plant according to the invention, while maintaining the genetic determinants for small diploid fruits. For example other fruit characteristics as described above can be introduced (e.g. darker red flesh color, higher brix, firmer flesh, a different flesh color, etc.), or any other traits can be introduced, such as one or more QTLs for high yield, disease resistance genes, stress tolerance genes (e.g. water stress tolerance), etc. resistance to fungal-, bacterial-, viral-diseases, root-knot nematodes and/or insect pests may be introduced. For example, resistance to Fusarium wilt (Fusarium oxysporum fsp. niveum race 0, race 1 and/or race 2, and/or race 3, and/or other new races which may develop), Anthracnose (Colletotrichum lagenarium races 1-7, or other new races), Gummy stem blight, powdery mildew, Verticillium wilt, bacterial fruit blotch, papaya ringspot virus (PRSV), watermelon mosaic virus (WMV) or zucchini yellow mosaic virus (ZYMV).

(35) Resistance to Fusarium wilt races 0 and 1 is present in many commercial varieties, and also resistance to race 2 has been identified in PI296341 and PI271769 and is also present in SP-4 (U.S. Pat. No. 7,550,652). Anthracnose resistance to race 1 is for examples present in SP-4 and resistance to race 2 in AU-Sweet Scarlet (AW-82-50CS) (Breeder: Alabama Agric. Expt. Station, Auburn University). Crimson Sweet has the Ar-1 gene, which provides resistance to anthracnose races 1 and 3. Gummy stem blight resistance is also found in Plant Introduction lines.

(36) Also provided is a method for producing diploid and triploid watermelon fruits in one field, said method comprising: (a) interplanting diploid pollenizer plants according to the invention and triploid hybrid plants in one field, (b) allowing pollination of flowers of the triploid hybrid plants with pollen of the diploid pollenizer plants and allowing pollination of flowers of the diploid pollenizer plants with pollen of the diploid pollenizer plants, (c) harvesting fruits produced on the triploid hybrid plants and, optionally, harvesting fruits produced on the diploid pollenizer plants.

(37) In one embodiment of the invention the diploid fruits and/or the triploid fruits are edible and red-fleshed, white-fleshed, orange-fleshed or yellow-fleshed.

(38) In a further embodiment the rind of the diploid fruits is not yellow or Golden (as controlled by the recessive gene go) (Barham 1965, Proc Ameri Soc Hort Sci 67: 487-489).

(39) Interplanting in one field may be either done by seeding or transplants of the pollenizer and triploids. Various interplanting methods can be used, as known in the art and various ratios of pollenizer:triploid hybrid may be used. One row of pollenizer plants may for example be present at least every 2, at least every 3 or at least every 4 rows of triploids, but other methods of interplanting may also be used.

(40) Any triploid hybrid may be used, such as known triploid hybrid varieties.

(41) Pollination is usually done by bees, and bee hives can be provided to the fields unless sufficient wild bees are naturally present. Pollination can also be performed by manual or mechanical means. Harvest at maturity may be done by hand or mechanized.

(42) The diploid fruit may be distinguished from the triploid fruit based on the smaller fruit size of the diploid fruit, and/or alternatively by a different rind pattern. In one embodiment the rind of the diploid fruits according to the invention is not yellow or golden. Preferably harvested diploid and triploid fruit are placed into different containers. Thus, in one embodiment a container comprising solely small diploid fruits according to the invention is provided. Any type of container may be used, e.g. cartons, boxes, etc.

(43) Also a method for producing small diploid edible watermelon fruits having an average weight of less than 1.8 kg, 1.7 kg, 1.0 kg, 0.9 kg, 0.8 kg, 0.7 kg, preferably equal to or less than 0.65 kg (but in one embodiment larger than 0.25 kg), is provided comprising:

(44) (a) growing a plant according to the invention, i.e. a pollenizer as described herein,

(45) (b) pollinating the female flowers of said plant with pollen of said plant,

(46) (c) harvesting the fruits produced on said plant.

(47) Thus, by self-pollination of a diploid plant according to the invention, small edible diploid fruits are produced. The pollenizers according to the invention may be grown in a field without other watermelon plants being present, and the small diploid fruits may be harvested and placed in containers for transport. Step (b) may be performed by allowing insect pollination or any other means of pollinating.

(48) In one embodiment a diploid pollenizer plant (or a seed from which the plant can be grown) capable of producing small edible diploid fruits having an average weight of less than 1.8 kg, 1.7 kg, 1.0 kg, 0.9 kg, 0.8 kg, 0.7 kg or equal to or less than 0.65 kg (but in one embodiment larger than 0.25 kg) is provided, wherein a representative sample of seed containing the genetic elements for producing said small fruits has been deposited under accession number NCIMB 41773 (WH9307) or accession number 42704 (WH9716).

(49) Especially, pollenizer hybrid WH 9307 comprises the genetic elements for producing said small, edible fruits according to the invention and a representative sample of seeds have been deposited under accession number NCIMB 41773. Thus, when referring herein to seed deposits of pollenizers according to the invention, this pollenizer is referred to as a representative plant according to the invention, but seeds of the other hybrids or lines are also suitable and when reference to WH9307 or progeny thereof (or parts of any of these) is made, the other hybrids or lines mentioned herein are equally implied.

(50) In one embodiment a hybrid watermelon seed is provided, having as a male or female parent (preferably as a male parent) a diploid pollenizer plant capable of producing small edible diploid fruits having an average weight of less than 1.8 kg, 1.7 kg, 1.0 kg, 0.9 kg, 0.8 kg, 0.7 kg or equal to or less than 0.65 kg (but in one embodiment larger than 0.25 kg) is provided, wherein a representative sample of seed containing the genetic elements for producing said small diploid fruits has been deposited under accession number NCIMB 41773 (WH9307) or accession number 42704 (WH9716).

(51) In one embodiment the fruit of a cross-pollination between a diploid pollenizer plant as pollen donor capable of producing small diploid fruits having an average weight of less than 1.8 kg, 1.7 kg, 1.0 kg, 0.9 kg, 0.8 kg, 0.7 kg or equal to or less than 0.65 kg (but in one embodiment larger than 0.25 kg) is provided, wherein a representative sample of seed containing the genetic elements for producing said small fruits has been deposited under accession number NCIMB 41773 (WH9307) or accession number 42704 (WH9716) and the pistillate flowers of another watermelon plant is provided. The other watermelon plant is preferably a triploid hybrid and the fruit is triploid and preferably seedless.

(52) In one embodiment a diploid fruit and/or an inbred diploid seed is provided produced by self-pollinating a diploid pollenizer plant capable of producing small edible diploid fruits having an average weight of less than 1.8 kg, 1.7 kg, 1.0 kg, 0.9 kg, 0.8 kg, 0.7 kg or equal to or less than 0.65 kg (but in one embodiment larger than 0.25 kg) is provided, wherein a representative sample of seed containing the genetic elements for producing said small diploid fruits has been deposited under accession number NCIMB 41773 (WH9307) or accession number 42704 (WH9716).

(53) In one aspect of the invention plants according to the invention and fruits according to the invention are obtainable from WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, WH9721 or of progeny of any of these.

(54) Plants obtained (derived), or obtainable (derivable), from plants according to the invention (e.g. from deposited seeds) include, therefore, plants obtained by breeding methods, such as selfing, crossing, backcrossing, recurrent selection, double haploid production, marker assisted selection, clonal propagations, transformants, etc., whereby the derived plants produce small, edible fruits according to the invention.

(55) In another aspect of the invention plants having essentially all the morphological and/or physiological characteristics of WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, WH9721 or of progeny of any of these, are provided. Representative examples of physiological and morphological characteristics are provided in Tables 1-8.

(56) Also provided is a progeny plant of WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320 or WH9321, WH9716, WH9717, WH9721 obtained by further breeding with said plant, wherein said progeny plant has essentially all physiological and morphological characteristics of said plant.

(57) A plant comprising a first set of chromosomes of any of the plants WH9716, or WH9717, or WH9721, a representative sample of seeds of WH9716 having been deposited under Accession Number NCIMB 42704.

(58) A seed comprising a first set of chromosomes of any of the plants WH9716, or WH9717, or WH9721, a representative sample of seeds of WH9716 having been deposited under Accession Number NCIMB 42704.

(59) Essentially all physiological and morphological characteristics include herein at least small, edible fruits and dual purpose pollenizer characteristics as described throughout the description and examples. Thus, in one aspect, essentially all physiological and morphological characteristics refer herein to a plant having the fruit characteristics and/or the flowering (pollenizer) characteristics as described in specification and the Examples.

(60) Also a plant part (e.g. a fruit, tissue, cell, cell culture, vegetative propagation, pollen, etc.) is provided which, when regenerated or grown into a whole plant, has essentially all the morphological and/or physiological characteristics of WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320 or WH9321, WH9716, WH9717, WH9721.

(61) Also provided is a plant derived from (or obtained from) or derivable from (or obtainable from) any of WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320 or WH9321, WH9716, WH9717, WH9721 having one or two physiological and/or morphological characteristics which are different from the WH plant listed and which otherwise has essentially all physiological and morphological characteristics of a plant designated WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320 or WH9321, WH9716, WH9717, WH9721 obtainable by further breeding with the WH plant and/or by selecting a natural or induced mutant, or a somaclonal variant from a population of plants designated WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320 or WH9321 WH9716, WH9717, WH9721.

(62) Thus, the invention provides a seed of watermelon variety WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721.

(63) The invention provides a plant of watermelon variety WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721, or a part thereof.

(64) The invention provides a fruit of watermelon variety WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721, or a plant part produced from the plant above.

(65) The invention provides a method of producing a watermelon plant, comprising crossing any one of the plants of WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721 with a second watermelon plant one or more times, and selecting progeny from said crossing.

(66) The invention provides a method of producing a watermelon plant, comprising selling any one of the plants of WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721 one or more times, and selecting progeny from said selling.

(67) The invention provides progeny of watermelon variety WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721 obtained by further breeding with said variety.

(68) The invention provides the progeny of watermelon variety WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721, wherein said progeny have all the physiological and morphological fruit and/or flowering characteristics of watermelon variety WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721 respectively when grown under the same environmental conditions.

(69) The invention provides the progeny of watermelon variety WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721, wherein said progeny have all the physiological and morphological characteristics of watermelon variety WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721, respectively, when grown under the same environmental conditions.

(70) The invention provides an Essentially Derived Variety of any one of WH9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721 having one, two or three physiological and/or morphological characteristics which are different from those of WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721 and which otherwise has all the physiological and morphological characteristics of WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721.

(71) The invention provides a method of producing plants, or a part thereof, of variety WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721 comprising vegetative propagation of variety WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721, respectively.

(72) In one aspect said vegetative propagation comprises regenerating a whole plant from a part of variety WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721.

(73) In one aspect, said part is a cutting, a cell culture or a tissue culture.

(74) The invention provides a vegetative propagated plant of variety WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721, or a part thereof, having all the morphological and physiological characteristics of WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721, respectively, when grown under the same environmental conditions.

(75) The invention provides a plant part derived from variety WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721, or from the vegetatively propagated plant above, wherein said plant part are harvested fruit or parts thereof, pollen, cells, leaves or parts thereof, petioles, shoots or parts thereof, stems or parts thereof, roots or parts thereof, cuttings, or flowers or parts thereof.

(76) The invention provides a food or feed product comprising such a plant part. The plant part is fresh or processed.

(77) The invention provides a watermelon plant produced by growing the seed of WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721.

(78) The invention provides a method of producing a watermelon plant having a desired trait, wherein the method comprises transforming the watermelon plant WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721 with a transgene that confers the desired trait, wherein the transformed plant retains all the phenotypic and morphological characteristics of variety WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721, respectively, and contains the desired trait.

(79) The invention provides a watermelon plant produced by the method above, wherein the plant comprises the desired trait and all of the physiological and morphological characteristics of WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721. The invention provides a watermelon plant comprising at least a first set of the chromosomes of watermelon line WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721, and further comprising a single locus conversion, wherein said plant has essentially all of the morphological and physiological characteristics of the plant comprising at least a first set of the chromosomes of watermelon line WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721.

(80) The single locus conversion in one aspect confers a trait of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, environmental stress tolerance, modified carbohydrate metabolism, modified protein metabolism, or any combination thereof.

(81) The invention also provides use of any one of WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721, or progeny of any one of these, for providing pollen to produce triploid, seedless watermelon fruits.

(82) The invention thus also provides triploid fruits produced from one of WH 9306, WH9307, WH9308, WH9309, WH9322, WH9313, WH9317, WH9318, WH9319, WH9320, WH9321, WH9716, WH9717, or WH9721, or progeny of any one of these, as male parent (pollenizer) and a triploid watermelon plant as female parent.

DEPOSIT INFORMATION

(83) Applicant(s) maintain a deposit of at least 2500 seeds of hybrid pollenizers mentioned in the Examples, and parent inbred lines, at Nunhems B. V. Applicant has deposited hybrid WH 9307 at the NCIMB on 12 Nov. 2010 under Accession number NCIMB 41773, and WH 9716 at the NCIMB on 12 Dec. 2016 under Accession number NCIMB 42704. Access to this deposit will be available during the pendency of this application to persons determined by the Commissioner of Patent and Trademarks to be entitled thereto upon request.

(84) Subject to 37 C.F.R. §1.808(b), all restrictions imposed by the depositor on the availability to the public of one or more deposits will be irrevocably removed upon the granting of the patent by affording access to a deposit of at least 2500 seeds with the American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, Va. 20110 or National Collections of Industrial, Food and Marine Bacteria (NCIMB), 23 St. Machar Drive, Aberdeen, Scotland, AB24 3RY, United Kingdom. The deposit will be maintained for a period of 30 years, or 5 years after the most recent request, or for the enforceable life of the patent whichever is longer, and will be replaced if it ever becomes nonviable during that period. Applicant does not waive any rights granted under this patent on this application or under the Plant Variety Protection Act (7 USC 2321 et seq.).

(85) The following non-limiting Examples describe the production of diploid pollenizers and small, edible diploid fruits according to the invention. Unless stated otherwise in the Examples, methods for conventional watermelon breeding are used, such as e.g. described in Maynard 2001, Watermelons—Characteristics, Production and Marketing, ASHS Press; Mohr H. C. Watermelon Breeding in Mark J. Bassett (editor) 1986 Breeding Vegetable Crops, AVI Publishing Company.

EXAMPLES

Example 1—Breeding History

(86) Breeding for the hybrids goes back to 1985, to in-house breeding lines and crosses of these with the variety Allsweet. Selected self-pollinations were backcrossed with Tomato Seed OP (Sugar Baby mutant; ts gene) and selections were made for red flesh and small seed. Further self-pollinations were selected for red flesh, small seeds and agronomic traits in general. Selections were crossed with a line with many male flowers, followed by several selfings and selection for the production of many male flowers and small red fruits.

Example 2—Pollenizer Characteristics

(87) 2.1—Materials and Methods

(88) A field trial was conducted in Italy (Sant'Agata Bolognese-BO). Seeds were sown on 7 Apr. 2010 and transplanted into the field on 21 May 2010 (100 cm within the row, 250 cm between the rows). The plot contained 10 plants per line. Fruits were harvested on 26/27 Jul. 2010 for evaluation.

(89) 2.1.1 Flowering

(90) The number of open male and female flowers were counted for three plants per line on the flowering date (day 1=24 Jun. 2010), and 8, 15 and 22 days after the flowering date (day 8=2 Jul. 2010; day 15=9 Jul. 2010; day 22=16 Jul. 2010). The mean number of three plants per line was calculated.

(91) 2.1.2 Fruits

(92) 1) Average fruit number: at maturity (26/27 July) the total number of fruits harvested from two plants was counted and the average fruit number determined.

(93) 2) Average fruit weight: mean of the weight of three fruits per line randomly harvested from two plants at maturity

(94) 3) Brix: Value is the mean of three reading for three fruit, collected between the centre and the rind of the fruit; expressed in Degrees Brix)(° using the K71901 portable refractometer Mod. RLC ATC 0-18% (OPTECH).

(95) 4) Flesh structure (flesh firmness): Value is the mean of the reading of three fruits; expressed in kg using the fruit pressure tester FT 011 (Cientec Instrumentos)

(96) 5) Flesh colour: evaluated using the Royal Horticultural Society mini colour chart (http://www.rhs.org.uk/Plants/RHS-Publications/RHS-colour-charts)

(97) 2.1.3 Other Fruit Characteristics

(98) Measurements were done for three fruits:

(99) 1) Fruit length (cm), indicated as FRT-cm_L

(100) 2) Fruit diameter at midsection (cm), indicated as FRT-cm_D

(101) 3) Fruit rind thickness (cm), blossom end, indicated as RND-cm_BE

(102) 4) Fruit rind thickness (cm), side of fruit, indicated as RND-cm_S.

(103) Rind thickness is measured from the outer edge of the fruit to the boundary between white mesocarp and colored endocarp.

(104) 2.2—Results

(105) 2.2.1—Fruit Characteristics

(106) TABLE-US-00001 TABLE 1 Fruit characteristics of fruits from 11 diploid pollenizers according to the invention and commercial diploid pollenizers (Sidekick, SP-4 and Polimax) Average Fruit Dual Purpose fruit flesh Pollenizers Average weight structure Flesh color (diploid hybrids) fruit no. (kg) ° Brix (kg) (RHS) WH 9306x 38.5 0.52 7.0 0.6 Red (41A) WH 9307x 41.0 0.55 8.5 1.4 Red (41A) WH 9308x 51.0 0.44 7.7 0.6 Red (41B) WH 9309x 38.5 0.45 8.0 0.6 Red (41A) WH 9311x 56.5 0.55 7.3 0.6 Red (41B) WH 9313x 23.5 0.59 6.3 0.5 Red (39B) WH 9317x 51.5 0.45 5.7 0.6 Red (41B) WH 9318x 34.5 0.51 7.7 0.6 Red (39B) WH 9319x 52.5 0.57 7.3 0.6 Red (39B) WH 9320x 35.5 0.49 7.7 0.5 Red (41B) WH 9321x 49.5 0.33 6.2 0.5 Red (39B) Min-Max 23.5-56.5 0.33-0.59 5.7-8.5 0.5-1.4 Red (RHS 39B-41A) (average) (43.0) (0.49) (7.2) (0.6) Commercial pollenizers: Sidekick 27.5 0.75 7.7 0.6 Pink (31D) SP-4  8.5 1.4  5.2 1.1 white Polimax  5.0 2.3  10.3  1.0 red

(107) As can be seen from Table 1, the diploid pollenizers according to the invention produced very small, red-fleshed edible fruits. Fruit weight in Table 1 is significantly smaller than that of commercial pollenizers such as Sidekick, SP-4 and Polimax.

(108) TABLE-US-00002 TABLE 2 Fruit dimensions Average length (cm) Average width (cm) Pollenizers (diploid hybrids) (FRT-cm_L) (FRT-cm_D) WH 9306x 11.33 10.17 WH 9307x 10.50 9.83 WH 9308x 10.50 9.67 WH 9309x 11.00 10.00 WH 9311x 10.38 9.88 WH 9313x 11.33 10.00 WH 9317x 10.83 9.17 WH 9318x 11.17 9.67 WH 9319x 10.67 9.33 WH 9320x 11.33 9.17 WH 9321x 9.67 8.67 Commercial pollenizers: Sidekick 12.00 11.33 SP-4 15.33 13.17 Polimax 16.50 16.00

(109) Table 2 shows that the pollenizers according to the invention have on average smaller fruit dimensions than the commercial pollenizers. The fruit length is on average 11.33 cm or smaller, while in Sidekick the average fruit length is 12 cm. The diameter is also smaller, only 10.17 cm or less, compared to 11.33 cm in Sidekick. Thus fruit dimensions of equal to or below 11.33×10.17 cm (e.g. even as small as 9.67×8.67 cm) are significantly smaller than 12.00×11.33 cm in Sidekick.

(110) Table 3 shows mean rind thicknesses per line, measured at two points of the fruit. Thin rinds are an advantage for consumption.

(111) TABLE-US-00003 TABLE 3 Rind thickness at the blossom end and on the side of the fruit Average Average rind Pollenizers rind thickness - blossom end thickness - side (cm) (diploid hybrids) (cm) (RND-cm_BE;) (RND-cm_S) WH 9306x 0.17 0.37 WH 9307x 0.27 0.43 WH 9308x 0.20 0.37 WH 9309x 0.20 0.40 WH 9311x 0.25 0.40 WH 9313x 0.23 0.47 WH 9317x 0.15 0.33 WH 9318x 0.20 0.30 WH 9319x 0.23 0.40 WH 9320x 0.20 0.40 WH 9321x 0.13 0.30 Commercial pollenizers: Sidekick 0.30 0.43 SP-4 0.10 0.10 Polimax 0.40 0.70

(112) The pollenizers according to the invention have good, although thin, rind thickness of between 0.30 and 0.47 cm. The rind is also not susceptible to cracking, giving the fruits good handling properties. Fruits do also not have a brittle or explosive rind.

(113) The rind pattern of the fruits of all pollenizers according to the invention is a Crimson Sweet type rind pattern (medium-striped or netted), but the small fruit size can also be combined with other rind colors using standard breeding methods.

(114) 2.2.2—Flowering Characteristics

(115) TABLE-US-00004 TABLE 4 Flowering characteristics of 11 diploid pollenizers according to the invention and commercial diploid pollenizers (Sidekick, SP-4 and Polimax) No. of open male flowers No. of open female flowers Day 1 Day 8 Day 15 Day 22 Day 1 Day 8 Day 15 Day 22 Pollenizer WH 9306x 18.3 48.3 49.0 35.7 4.7 2.0 0.0 1.3 WH 9307x 15.0 51.0 53.7 35.0 4.7 3.0 0.3 1.0 WH 9308x 12.3 45.0 36.0 41.7 4.3 4.7 0.0 2.0 WH 9309x 10.0 42.3 49.7 46.7 2.0 1.0 0.3 2.3 WH 9311x 11.7 54.7 57.0 61.7 1.3 6.3 0.7 3.0 WH 9313x 9.0 57.3 47.7 40.3 5.0 2.7 0.3 1.3 WH 9317x 12.0 56.7 68.3 82.3 5.0 4.7 0.7 5.3 WH 9318x 7.0 38.7 65.3 68.7 4.7 5.7 0.0 5.3 WH 9319x 18.3 71.7 54.3 56.7 3.7 3.3 0.3 1.3 WH 9320x 12.0 43.7 47.7 33.3 3.3 2.0 0.0 0.7 WH 9321x 13.3 42.7 65.0 56.7 4.3 5.3 0.0 3.3 Min-Max 7.0-13.8 38.7-71.7 36.0-68.3 33.3-82.3 1.3-5.0 1.0-6.3 0.0-0.7 0.7-5.3 (average) (12.6) (50.2) (54.0) (50.8) (3.9) (3.7) (0.2) (2.5) Commercial pollenizers: Sidekick 14.3 46.3 33.0 24.3 2.0 4.7 0.0 0.0 SP-4 13.3 11.7 29.7 28.7 2.3 1.3 2.3 1.0 Polimax 14.3 10.0 18.3 18.7 1.3 1.7 1.3 1.3
2.2.3—Vegetative Types

(116) The hybrids have a relatively compact growth type, with the (average/pollenizer line of the) longest branch falling between 113.5 and 180.0 cm long and the shortest branch being between 57.0 and 80.0 cm long. The average number of primary branches per pollenizer line was between 3 and 3.5. The average number of secondary branches per pollenizer line at 30 cm and at 90 cm was between 56.0 and 88.5 (at 30 cm) and between 77.5 and 144.0 cm. Internode length ranged between 4.3 and 5.3 on average, depending on the line. Leaf length and width is also relatively compact, with leaf widths between 7.1 and 9.4 cm and lengths between 7.8 and 9.1 cm.

Example 3—Use of Pollenizers According to the Invention

(117) 3.1—Trial Set-Up

(118) Three trials were carried out in Spain using pollenizers WH9317x, WH9318x, WH9320x and WH9321x for triploid fruit production on the triploid hybrid variety ‘Fashion’.

(119) Trial 1:

(120) Location: green house

(121) Trial dimensions: 3600 m.sup.2

(122) Transplanting date: 19 Mar. 2010

(123) Harvest date: 16 Jun. 2010

(124) Scheme: pollenizers and triploids were in separate rows, alternating one row of triploid and one row of pollenizer. Distance between rows was 3 meter, distance between plants in a row was 1 meter.

(125) Trial 2:

(126) Location: open field

(127) Trial dimensions: 2500 m.sup.2

(128) Transplanting date: 24 Mar. 2010

(129) Harvest date: 6 Jul. 2010

(130) Scheme: pollenizers and triploids were interplanted in the same rows, with pollenizers making up 25% of the total plants. Distance between rows was 3 meters and distance between plants in a row was 1 meter.

(131) Trial 3:

(132) Location: open field

(133) Trial dimensions: 1500 m.sup.2

(134) Transplanting date: 20 Mar. 2010

(135) Harvest date: 2 Jul. 2010

(136) Scheme: pollenizers and triploids were interplanted in the same rows, with pollenizers making up 25% of the total plants. Distance between rows was 2 meters and distance between plants in a row was 1.8 meter.

(137) 3.2—Trial Results

(138) TABLE-US-00005 TABLE 5 mean value* of the triploid fruit weight (kg) of Fashion (triploid hybrid) for three trials carried out in three locations in Spain 2010 Trial 1 Trial 2 Trial 3 WH9317x 5.14 4.18 4.27 WH9318x 5.06 4.48 4.12 WH9320x 5.22 3.94 4.23 WH9321x 4.65 4.35 4.30 Average 5.02 4.24 4.23 (min-MAX) (2.5-9.08) (2.5-7.64) (2.56-7.60) Commercial Pollenizers Jenny 5.15 4.26 4.96 Polimax 5.30 3.90 4.54 SP-4 6.14 4.26 4.44 Sidekick 5.35 *Mean value: mean of the weights of the total marketable triploid fruits (>2.5 kg) harvested in an area of 60 m.sup.2, 90 m.sup.2 and 30 m.sup.2 of the Trial 1, Trial 2 and Trial 3, respectively.

(139) The trial results of Table 5 show that the dual purpose pollenizers of the invention are indeed good pollenizers and lead to good triploid fruit yields.

Example 4

(140) Three further diploid hybrids were developed having dual purpose pollenizer characteristics as described for the above hybrids but having a higher brix content and being thus particularly suitable for fresh consumption similar to apples.

(141) Fruit characteristics—harvested at maturity (method for measurements as in examples 1 and 2)

(142) TABLE-US-00006 TABLE 6 Average Dual Purpose fruit Fruit flesh Pollenizers Average weight structure Flesh color (diploid hybrids) fruit no. (kg) ° Brix (kg) (RHS) WH9716 61.7 0.5 8.0 0.7 Red (44A) WH9717 65.7 0.7 10.1 0.8 Red (41A) WH9721 57.3 0.5 9.2 0.7 Red (41A) Comparison 9.3 2.4 10.0 1.2 Red (44A) Polimax

(143) TABLE-US-00007 TABLE 7 Average length (cm) Average width (cm) Pollenizers (diploid hybrids) (FRT-cm_L) (FRT-cm_D) WH9716 10.0 9.2 WH9717 10.4 9.7 WH9721 11.0 9.7 Comparison 17.0 16.0 Polimax

(144) TABLE-US-00008 TABLE 8 Average Average rind Pollenizers rind thickness - blossom end thickness - side (cm) (diploid hybrids) (cm) (RND-cm_BE;) (RND-cm_S) WH9716 0.2 0.2 WH9717 0.1 0.3 WH9721 0.1 0.3 Comparison 0.5 0.5 Polimax

(145) These three dual purpose pollenizers thus produce small diploid edible fruits on the pollinizer plants, which are particularly suitable for being sold for fresh consumption.

Example 5

(146) In another experiment fruit characteristics of the three diploid hybrid pollenizers given in example 4 have been determined.

(147) Trial 4:

(148) Location: Open Field, Acampo Calif. USA N32° 46.530′, W115° 33.017′, Elevation 16 m, Trial dimensions: 472 m.sup.2.

(149) Transplanting date: 6 Aug. 2012; Harvest date: 17 Oct. 2012.

(150) Scheme: The distance between rows (beds) were 5′, the distance between plants, 3′.

(151) Fruit characteristics harvested at maturity (Measurement method: see examples 1 and 2). “USDA descriptors” are the plant variety descriptors described for watermelon in the “Objective description of Variety Watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai”, ST-470-18 (dated Jul. 1, 2009) as published by U.S. Department of Agriculture, Agricultural Marketing Service, Science and Technology, Plant Variety Protection Office, Beltsville, Md. 20705 (available and which can be downloaded on the world wide web at usda.gov).

(152) TABLE-US-00009 TABLE 9 Characteristics WH WH WH Poli- 1)-14) USDA descriptor 9716 9717 9721 max 1. General Fruit Type 1 = Oblong, 2 = Round Large, 3 = Round 4 4 4 3 small (icebox), 4 = round mini 2. Area of best adaptation 1 = Southern US, 2 = Northeast/Central US, 4 4 4 4 3 = Southwest US, 4 = Most US Areas 5 = other 3. Maturity No. of days from emergence to anthesis — — — — No. of days from pollination to maturity 33 33 33 35 Days Relative Maturity (as reported in seed 75 75 75 78 catalogs) Maturity category 1 = early, 2 = medium, 1 1 1 1 3 = late 4. Ploidy 1 1 1 1 1 = diploid, 2 = tetraploid, 3 = triploid 5. PLANT Cotyledon shape: 1 = flat, 2 = folded 1 + 2 1 + 2 1 + 2 1 + 2 Plant sex form: 1 = monoecious, 2 = 1 1 1 1 andromonoecious No. of main stems at crown 3 3 3 3 1 Number of flowers per plant at first fruit set: 25.6 35.8 31.8 7.8 Staminate Number of flowers per plant at first fruit set: 1.6 2.4 3.4 3 Pistillate Number of flowers per plant at first fruit set: 0 0 0 0 Perfect 6. STEM Stem shape (cross section): 1 = round, 2 = 2 2 2 2 angular 8 Diameter (mm) at second node 9.4 8.9 8.6 7.2 Stem surface: 3 3 3 3 1 = glabrous, 2 = scabrous, 3 = pubescent, 4 = bristled 2 Vine length (cm) (at last harvest) 109.8 128.3 110.5 155.1 3 No. of Internodes (at last harvest) 19 22 20.8 19 Ratio Vine length (cm): No of internodes (at 5.8 5.8 5.3 8.2 last harvest) 7. LEAF Leaf shape: 1 = ovate, 2 = obovate, 3 = round 1 1 1 1 Leaf lobes: 1 = none, 2 = lobed 2 2 2 2 Leaf length (cm) 16.4 16.0 12.7 15.5 Leaf width (cm) 13.3 13.7 11.4 13.9 Leaf size: 1 = longer than wide, 2 = length- 1 1 1 1 width equal, 3 = wider than long Dorsal surface pubescence: 1 = smooth, 2 = 2 2 2 2 pubescent Ventral surface pubescence: 1 = smooth, 2 = 2 2 2 2 pubescent Leaf color: 1 = light green, 2 = gray green, 3 = 3 3 3 2 medium green, 4 = dark green Color chart name: RHS; Value 147B 137B 137B 138B 8. FLOWER Diameter across Staminate (cm) 2.8 3.2 2.9 3.1 Diameter across Pistillate (cm) 2.2 2.96 2.5 3.3 Flower color: 1 = lemon yellow, 2 = yellow, 3 = 2 2 2 2 orange Color chart name: RHS; Value 7B 7B 7B 7A 9. MATURE FRUIT Fruit shape: 1 = round, 2 = oval, 3 = 1 2 2 2 cylindrical 4 (cm) Long 10.7 11.4 12.0 19.6 5 Diameter at midsection (cm) 10.1 10.1 9.9 16.5 6 Average weight (kg) 0.60 0.647 0.665 2.9 7 Maximum fruit weight (kg) 0.81 0.83 0.848 3.7 Index = length ÷ diameter × 10 10.6 11.3 12.1 11.9 Fruit surface: 1 = smooth, 2 = slightly 1 1 1 1 grooved, 3 = deeply grooved Skin color pattern: 1 = solid (one color), 2 = 2 2 2 3 stripe, 3 = mottle/net Primary color: 1 = Yellow Green (Desert 4 4 4 5 King), 2 = Light Green (Charleston Gray), 3 = Medium Green (Sugar baby), 4 = dark green (Florida Giant), 5 = Gray Green Color chart name: RHS; Value: N137A N137A N137A 138C Secondary color 1 = Yellow Green, 2 = Light 1 1 1 3 Green, 3 = Medium green, 4 = dark green Color chart name: RHS; Value: 145D 145D 145D 146A 10. RIND Rind texture: 1 = tender, 2 = brittle, 3 = tough 2 2 2 2 9 Thickness blossom end (mm) 2.6 2.0 2.4 5.4 10 Thickness sides (mm) 4.4 3.4 4.1 8.6 11. FLESH Flesh texture: 1 = crisp, 2 = soft 2 2 2 2 Flesh coarseness: 1 = course fibrous, 2 = fine - 2 2 2 2 little fiber 11 Flesh color: 1 = white, 2 = yellow, 3 = 5 5 5 3 orange, 4 = pink, 5 = red, 6 = dark red 12 Refractometer: % Soluble solids of juice 8.4 8.4 9.0 10.1 (Center of fruit) (Brix) % Hollow heart 0 0 0 0 13 % placental separation 0 0 0 6.7 14 % traverse crack 0 0 0 6.7 12. SEED Seed Size: 1 = Small, 2 = Medium, 3 = Large 1 1 1 1 Length (mm) 5.25 5.02 4.92 5.17 Width (mm) 3.34 3.45 3.19 3.32 Thickness (mm) 1.62 1.70 1.81 1.63 Index = Length ÷ Width × 10 15.72 14.55 15.42 15.57 gm per 1000 Seeds 10 11.5 11.8 10.8 No. of seeds per Fruit 568 236 372.9 2430 Seed Color: 1 = white, 2 = white-tan tipped, 3 = 4 + 8 8 7 8 white-pink tipped, 4 = tan, 5 = green, 6 = red, 7 = dark brown, 8 = dark brown mottled, 9 = black, 10 = mottled black, 11 = other (specify)       Color chart name: RHS, Value 161C + 165A 165A 165A 165A

(153) Characteristics 1-14 are characteristics in which the diploid pollenizer hybrids of the invention differ (statistically) significantly from the most similar pollenizer Polimax.

(154) These are typical values. Values may vary due to environment. Other values that are substantially equivalent are also within the scope of the invention. For example, Brix is environmentally sensitive which explains the slight differences in brix of ripe fruit between Examples 4 and 5.

(155) All of the patent documents (e.g., patents and published applications) and non-patent literature documents referred to herein are hereby incorporated by reference herein in their entireties.