QUANTIFICATIONAL TESTING METHOD FOR SEMEN LIQUEFACTION ABILITY

Abstract

This invention publicly announces a quantificational testing method of semen liquefaction ability. By following the steps of sample preparation, image collection, image quantificational analysis and curve analysis to analyze the quantificational testing of semen liquefaction ability; indirectly indicate the semen liquefaction ability by observing the color depth change in the sperm sampled cover black area; to analyze the quantification of semen liquefaction by observing the level of change in grey level value versus time; thus, to efficiently, subjectively and standardly test the semen liquefaction ability and avoid human errors.

Claims

1-10. (canceled)

11. A quantificational testing method of semen liquefaction ability, comprising: Step 1: sample preparation by adding a semen sample to testing sample system; installing black sample pool inside the sample's system; adding semen sample to the sample pool; Step 2: image collection beginning from adding semen sample to the sample pool, collecting the semen sample images of the sample pool by following the time order to receive analyzing images; Step 3: sample quantification analysis by calculating each image's gray level value to gain gray value versus time graph; wherein the ratio difference of the graph represents the change of color on the image within the timeline; Step 4: graph analysis wherein semen liquefaction ability is related to image's change of color; the graph also represents sperm liquefaction ability; the semen liquefaction ability analysis is gained by obtaining gray level value change within unit time; wherein more change of gray level value within unit time represents stronger semen liquefaction ability, less change of gray level value within unit time represents weaker semen liquefaction ability.

12. The method of claim 11, wherein one of the quantification testing method for semen liquefaction ability, its feature is as following: during step 1, the height of sample pool is 2 to 5 millimeters.

13. The method of claim 12, further comprising: during step 1, the transparent cover plate is placed on top of the sample pool.

14. The method of claim 11, wherein during step 2, the images are collected at the same height above the sample pool.

15. The method of claim 14, wherein during step 2, the images are collected at the same time gaps.

16. The method of claim 15, wherein during the step 2, the time gap is between 5 to 60 seconds.

17. The method of claim 11, wherein during step 2, the collection of images is operated under stale lighting conditions.

18. The method of claim 11, wherein during step 3, choosing the same and fixed area of each image to calculated the area's grey level value in order to analyze the grey level value versus time graph.

19. The method of claim 18, wherein during step 3, utilizing RBG color system, eight-digit image calculating grey level value and pure white grey level value at 225, and the actual estimated entire grey level value of assigned area is calculated by using formula 1, wherein $\begin{array}{cc}\mathrm{Grey}.Math..Math.\mathrm{Level}.Math..Math.\mathrm{Value}=\underset{i=1}{\overset{n}{.Math.}}.Math..Math.x_i;& \mathrm{Formula}.Math..Math.1\end{array}$ the actual estimated average grey level value of assigned area is calculated by using formula 2, wherein $\begin{array}{cc}\mathrm{Grey}.Math..Math.\mathrm{Level}.Math..Math.\mathrm{Value}=\underset{i=1}{\overset{n}{.Math.}}.Math..Math.x_i/n;& \mathrm{Formula}.Math..Math.2\end{array}$ the semen sample's entire grey level value of assigned area is calculated by using formula 3, wherein $\begin{array}{cc}\mathrm{Grey}.Math..Math.\mathrm{Level}.Math..Math.\mathrm{Value}=\underset{i=1}{\overset{n}{.Math.}}.Math..Math.\left(255-x_i\right);& \mathrm{Formula}.Math..Math.3\end{array}$ the semen sample's entire average value of assigned area is calculated by using formula 4, wherein $\begin{array}{cc}\mathrm{Grey}.Math..Math.\mathrm{Level}.Math..Math.\mathrm{Value}=\underset{i=1}{\overset{n}{.Math.}}.Math..Math.\left(255-x_i\right)/n;& \mathrm{Formula}.Math..Math.4\end{array}$ and during the calculation, n is a pixel number of an assigned area; and xi is an actual estimated gray level of in the assigned area.

20. The method of claim 11, wherein sample testing system includes a testing card, light source above the testing card; the sample pool is the place at the bottom of cover groove; the bottom of the sample pool is black; a transparent cover is place in the testing pool's cover groove; the testing card has a surplus sample containing groove; the surplus sample containing groove and sample pool are connected by a surplus flow groove; one end of the surplus flow groove is connected to the top of the sample pool; there is stable placement of an image collector on top of the sample pool; the image collector is able to connect to cellphone by wire or wireless connection; and the cellphone is connected to long distant computer.

Description

DESCRIPTION OF FIGURES

[0036] FIG. 1: procedure image of the method of testing quantification of the semen liquefaction ability in this invention;

[0037] FIG. 2: structural image of the testing sample system in this invention;

[0038] FIG. 3: structural image of reagent card 1 in FIG. 2;

[0039] FIG. 4: upward view of FIG. 3;

[0040] FIG. 5: the grey level value versus time graph obtained by experimental sample 1's data;

[0041] FIG. 6: the grey level versus time graph obtained by experimental sample 2's data;

[0042] FIG. 7: the grey level versus time graph obtained by experimental sample 3's data;

DETAILED IMPLEMENTING METHOD

[0043] ONE. To further explain this invention by the combination of implementing examples and figures.

[0044] According to FIG. 1, a method of testing the quantification of semen liquefaction by the following steps:

[0045] Step 1: Sample Preparation

[0046] Adding semen sample into testing sample system within 2 minutes of semen come out from the penis; placing black bottom sample pool inside the sample system; adding semen sample into the sample pool; semen sample is placed 2-5 millimeters in the sample pool; after placing the semen sample into the sample pool, covering the sample pool with transparent cover.

[0047] Step 2: Image Collection

[0048] Beginning with semen sample being added to the sample pool, collecting sample pool's semen sample images at the same height above the semen pool at time gap between 5-60 seconds.

[0049] When collection the images, the process must undergo under stable natural light source; if the natural sunlight is unstable, the process must undergo under stable artificial light source.

[0050] Step 3: Image Quantification Analysis

[0051] Choosing the same stable area of each analyzed image to form calculation area; utilizing RBG color system and 8-digit to calculate the area's grey level; the standard pure with grey level is 255 at this time.

[0052] The actual estimated total grey level value in the appointed calculating area is calculated by using formula 1:

[00005]$\begin{array}{cc}\mathrm{Grey}.Math..Math.\mathrm{Level}.Math..Math.\mathrm{Value}=\underset{i=1}{\overset{n}{.Math.}}.Math..Math.x_i& \mathrm{Formula}.Math..Math.1\end{array}$

[0053] The actual estimated average grey level value of assigned area is calculated by using formula 2.

[00006]$\begin{array}{cc}\mathrm{Grey}.Math..Math.\mathrm{Level}.Math..Math.\mathrm{Value}=\underset{i=1}{\overset{n}{.Math.}}.Math..Math.x_i/n& \mathrm{Formula}.Math..Math.2\end{array}$

[0054] The semen sample's entire grey level value of assigned area is calculated by using formula 3.

[00007]$\begin{array}{cc}\mathrm{Grey}.Math..Math.\mathrm{Level}.Math..Math.\mathrm{Value}=\underset{i=1}{\overset{n}{.Math.}}.Math..Math.\left(255-x_i\right)& \mathrm{Formula}.Math..Math.3\end{array}$

[0055] The semen sample's entire average value of assigned area is calculated by using formula 4.

[00008]$\begin{array}{cc}\mathrm{Grey}.Math..Math.\mathrm{Level}.Math..Math.\mathrm{Value}=\underset{i=1}{\overset{n}{.Math.}}.Math..Math.\left(255-x_i\right)/n& \mathrm{Formula}.Math..Math.4\end{array}$

[0056] In the calculation, n is number of appointed calculating areas' pixel spot; xi is actual estimated grey level value in the pointed calculating area's each digital pixel spot.

[0057] The grey level value versus time graph represents the analyzed image depth change degree within unit time.

[0058] Step 4: Graph Analysis

[0059] The ability of semen liquefaction is related to change in color depth of analyzing image; the change ratio of grey level value versus time graph represents the semen liquefaction ability; to analyze the quantification of semen liquefaction ability through change ratio of grey level within unit time; higher change ratio of grey level value within unit time presents stronger liquefaction ability, otherwise, weaker sperm liquefaction ability.

[0060] According to FIGS. 2, 3 and 4, the testing sample system includes testing card 1 and light source 3 above the testing card 1.

[0061] The testing card 1 is rectangular shaped, this testing card 1 is made of black plastic material; the testing pool's cover groove 1b is placed on top in the middle of the testing card 1; the sample pool 1a is placed on the bottom of the testing sample cover groove 1b; the sample pool 1a is hold like; surplus sample groove 1c is placed on one side of the testing pool's cover groove 1b on the testing card 1. The surplus sample groove 1c is connected to sample pool 1a by surplus flow groove 1d; one side of the surplus flow groove 1d is connected to hatch on the of the sample pool 1a; other side of the testing cover's groove 1b, the testing card 1 is control area; the testing cover's groove 1b would have placement of transparent cover 11.

[0062] The height of the sample pool 1a is 2-5 mm to maintain the same height as semen sample in the sample pool; after sperm sample is added to sample pool, adding the semen sample to fill up the sample pool 1a; then, close the transparent cover 11; relatively isolate the semen sample in the sample pool 1a; avoid some of the effects from out side on the semen liquefaction; surplus semen sample would follow through flow groove into surplus sample groove 1c without outside pollution; after closing the transparent cover 11; the semen sample is even with the height of sample pool 1a; semen sample maintains the same height in the sample pool 1a; to compare different semen samples' liquefaction abilities.

[0063] The image collector 2 is placed on top and in the middle of the sample pool 1a; the image collector 2 is image camera or video camera, it also could be other image collector; after adding the semen sample, the image collector 2 is collecting images from sample pool 1a; as the semen sample liquidizes, the transparency of the semen sample increases and blackness from the bottom of sample pool 1a increases; image collector 2 records the change in depth of color of the sample pool 1a in real time and sends the images information to cellphone; the cellphone sends the compressed images information to long distant computer via WIFI of mobile network; long distant computer finishes the images data analysis and grey level color value calculation to obtain testing results and reply back to cellphone's APP at live scene; As the alternative method of connection, the image collector could directly connect to processor; after the processor obtains the results, is directly presents the results to user; but, the device has a high cost which is not suitable for families; thus, utilizing cellphone as connection is more acceptable by family users.

[0064] If the natural light source is unstable, the light source 3 could be artificial light source, and the LED light source would be the best; the semen liquefaction ability's testing sample could be placed in the dark box at this time; surrounding the image collector 2 with the light source 3 to set up.

[0065] When the natural light source is stable, the light source 3 could utilize natural light source.

[0066] TWO. To further explain this invention with combination of experimental samples and figures in the followings:

[0067] Picking random semen samples from three people; utilizing the method of testing the quantification of semen liquefaction ability; each semen sample is under same conditions; mark the semen sample added into the sample pool as time 0; all three semen samples are collected from leaving the penis to time 0 without exceeding 2 minutes; starting from time 0, the semen samples' images are collected with the time gap of 30 seconds; stopping the image collection after 10 minutes; obtaining image analysis of each sample; point a stable area as calculating area for each image analysis; choosing 100 evenly distributed pixel points within the calculation area; utilizing RBG color system and 8-digit image to calcite the grey level of the calculating area; the standard pure white grey level is 225.

[0068] The actual estimated total grey level value in the appointed calculating area is calculated by using formula 1:

[00009]$\begin{array}{cc}\mathrm{Grey}.Math..Math.\mathrm{Level}.Math..Math.\mathrm{Value}=\underset{i=1}{\overset{n}{.Math.}}.Math..Math.x_i& \mathrm{Formula}.Math..Math.1\end{array}$

[0069] The actual estimated average grey level value of assigned area is calculated by using formula 2.

[00010]$\begin{array}{cc}\mathrm{Grey}.Math..Math.\mathrm{Level}.Math..Math.\mathrm{Value}=\underset{i=1}{\overset{n}{.Math.}}.Math..Math.x_i/n& \mathrm{Formula}.Math..Math.2\end{array}$

[0070] The semen sample's entire grey level value of assigned area is calculated by using formula 3.

[00011]$\begin{array}{cc}\mathrm{Grey}.Math..Math.\mathrm{Level}.Math..Math.\mathrm{Value}=\underset{i=1}{\overset{n}{.Math.}}.Math..Math.\left(255-x_i\right)& \mathrm{Formula}.Math..Math.3\end{array}$

[0071] The semen sample's entire average value of assigned area is calculated by using formula 4.

[00012]$\begin{array}{cc}\mathrm{Grey}.Math..Math.\mathrm{Level}.Math..Math.\mathrm{Value}=\underset{i=1}{\overset{n}{.Math.}}.Math..Math.\left(255-x_i\right)/n& \mathrm{Formula}.Math..Math.4\end{array}$

[0072] n the calculation, n is number of appointed calculating areas' pixel spot; n=100; xi is actual estimated grey level in the pointed calculating areas' each digital pixel spot; estimating the actual grey level of each pixel point is known by technicians within the field; thus, there no need to explain it in this content.

[0073] To obtain 3 semen samples by calculation at different times

[00013]$\underset{i=1}{\overset{n}{.Math.}}.Math..Math.x_i,$

as shown in table 1.

[0074] Thus, 3 semen samples at different times;

[0075] The actual estimated total grey value in the appointed calculating

[00014]$\mathrm{area}=\underset{i=1}{\overset{n}{.Math.}}.Math..Math.x_i;$

[0076] The actual estimated average grey value in the appointed calculating

[00015]$\mathrm{area}=\underset{i=1}{\overset{n}{.Math.}}.Math..Math.x_i/100;$

[0077] The actual relatively total grey value in the appointed calculating

[00016]$\mathrm{area}=25500-\underset{i=1}{\overset{n}{.Math.}}.Math..Math.x_i;$

[0078] The actual relatively average grey value in the appointed calculating

[00017]$\mathrm{area}=\left(25500-\underset{i=1}{\overset{n}{.Math.}}.Math..Math.x_i\right)/100=255-\underset{i=1}{\overset{n}{.Math.}}.Math..Math.x_i/100.$

TABLE-US-00001 TABLE 1 sperm samples at different times: Time/Second Sample 1 Sample 2 Sample 3 0 11745 12234 12097 30 9842 12035 12002 60 7696 11812 11979 90 5674 11657 11958 120 4017 11482 11913 150 3130 11297 11872 180 2724 11064 11834 210 2583 10880 11762 240 2332 10637 11740 270 2235 10473 11716 300 2196 10249 11690 330 2177 10032 11676 360 2165 9805 11634 390 2153 9614 11616 420 2149 9471 11575 450 2147 9255 11539 480 2147 9016 11509 510 2147 8854 11462 540 2147 8661 11377 570 2147 8479 11320 600 2146 8233 11290 630 2146 8045 11278 660 2146 7891 11265 690 2146 7617 11246 720 2146 7439 11237 750 2146 7236 11192 780 2146 7070 11156 810 2146 6821 11113 840 2145 6684 11053 870 2145 6425 11025 900 2145 6208 11013

[0079] According to data from table 1, utilizing formula 1, 2, 3 and 4 to calculate grey level value in order to obtain grey level value versus time graph.

[0080] For example, utilizing formula 2 to calculate grey level to draw the grey level value versus time graph from sample 1, 2 and 3; as shown in image 5, 6 and 7.

[0081] According to image 5, grey level value of sample 1 decreases as time increases; the grey level value has an obvious decrease within 200 seconds; change ratio of the graph is strong; this phenomenon indicates that semen liquidizes at highest rate within 200 seconds; the change ration of the graph is weaker after first 200 seconds; this phenomenon indicates that the semen liquidizes at lower rate; thus, sample 1 has strong semen liquefaction ability.

[0082] According to image 6, grey level value of sample 2 decreases as time increases; the grey level values weaker decrease within 200 seconds comparing to sample 1; change ration of the graph is also weaker than sample 1; until 900 seconds, change ration of the graph is still not obvious; the phenomenon indicates that the semen of sample 2 has not liquidized completely; thus, sample 2 has weak semen liquefaction ability.

[0083] According to image 7, grey level value of sample 3 does not decrease as the time increases; change ration of the graph is almost none; thus sample 3 does not have sperm liquefaction ability.

[0084] According to image 5, 6 and 7, through quantificational analysis, the semen liquefaction ability could be directly represented; the semen liquefaction speed within a particular time could be precisely obtained by calculating change ratio of grey level within a particular time; the semen liquefaction speed at a particular time could be precisely obtained by calculating change ratio of grey level (curve slope) at a particular time; thus, the semen liquefaction ability could be efficiently, subjectively and standardly estimated in order to eliminate human errors in the testing analysis.

[0085] By utilizing formula 4 to calculate grey level values to draw the grey level value versus time graphs of sample 1, 2 and 3, as indicated by FIGS. 8, 9 and 10.

[0086] By analyzing FIGS. 8, 9 and 10, estimation would be the same as discussed above.

[0087] Lastly, the description in the above is only preferred selection of examples of this invention. By being under the inspiration of this invention and obeying the purpose and rights of this invention, the technician within the field could present this invention in other similar ways; other presentations would be under the protection of this invention.