Bioluminescence resonance energy transfer between bioluminescent proteins and semiconductive nanomaterials
09758808 · 2017-09-12
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Abstract
A bioluminescence energy transfer (BRET) nanosystem having semiconductive quantum rods (QRs) bound by firefly luciferase Photinus pyralis (Ppy) for improved conversion of chemical energy to light, such as in solid-state lighting, near-infrared imaging systems, and in vivo infrared imaging. The nanosystems are formed by synthesizing CdSe/CdS or CdSe/CdS/ZnS quantum rods, rendering the dots hydrophilic and colloidially stable with a facile His-capping, incubating with a Ppy variant (PpyGRTS) at increasing loading ratios, and adding an excess of the luciferin (LH2) substrate to the PpyGRTS-QRs.
Claims
1. A bioluminescent energy transfer system, comprising: a semiconductive quantum rod having a rod-shaped core surrounded by a rod-shaped shell that fluoresces at a predetermined frequency; and at least one luciferase protein having a hexahistidine tag, wherein the hexahistidine tag binds the luciferase protein directly to the shell of the quantum rod via non-covalent coordination.
2. The system of claim 1, wherein said hexahistidine tag comprises an N-terminus hexahistidine tag.
3. The system of claim 2, wherein said luciferase comprises Photinus pyralis luciferase.
4. The system of claim 1, wherein said rod-shaped core comprises a CdSe rod-shaped core and said rod-shaped shell comprises a CdS rod-shaped shell.
5. The system of claim 4, wherein said quantum rod has an aspect ratio of at least 3.1.
6. The system of claim 1, wherein said quantum rod further comprises a layer of ZnS.
7. The system of claim 6, wherein said quantum rod has an aspect ratio of about 3.1.
8. The system of claim 1, wherein said predetermined frequency is in the infrared spectrum.
9. The system of claim 1, wherein said predetermined frequency is in the visible spectrum.
10. A method of emitting light, comprising the steps of: providing a bioluminescent complex having a semiconductive quantum rod a rod-shaped core surrounded by a rod-shaped shell that fluoresces at a predetermined frequency and at least one luciferase protein having hexahistidine tag that binds the luciferase protein directly to the surface of the quantum rod via non-covalent coordination; and exposing said bioluminescent complex to a substrate of said luciferase molecule.
11. The method of claim 10, wherein said hexahistidine tag resides at the N-terminus of said luciferase.
12. The method of claim 11, wherein said luciferase comprises Photinus pyralis luciferase.
13. The method of claim 11, wherein rod-shaped core comprises a CdSe rod-shaped core and said rod-shaped shell comprises a CdS rod-shaped shell.
14. The method of claim 13, wherein said quantum rod has an aspect ratio of about 3.1.
15. The method of claim 11, wherein said quantum rod further comprises a layer of ZnS.
16. The method of claim 15, wherein said quantum rod has an aspect ratio of about 3.0.
17. The method of claim 10, wherein said predetermined frequency is in the infrared spectrum.
18. The method of claim 10, wherein said predetermined frequency is in the visible spectrum.
Description
BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWING(S)
(1) The present invention will be more fully understood and appreciated by reading the following Detailed Description in conjunction with the accompanying drawings, in which:
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DETAILED DESCRIPTION OF THE INVENTION
(23) Referring now to the drawings, wherein like reference numerals refer to like parts throughout, there is seen in
(24) The assembly design and main structural concerns of a biotic-abiotic BRET nanosystem according to the present invention is further shown in
(25) Referring to 2(a), BRET was observed between the PpyGRTS donor, and CdSe/CdS QR acceptor with λA=675 nm (denoted as QR(675)). The TEM micrograph of the QR(675) revealed an aspect ratio (l/w) of 3.1±0.5 (1=24.8±3.6 nm, w=8.2±1.3 nm). The BRET energy transfer efficiency, BRET ratio (BR), was found to be highly susceptible to the molar loading ratio, L=[PpyGRTS]:[QR]. As seen in
(26) The resonance energy transfer efficiency of a donor/acceptor pair is highly sensitive to center-to-center (dipole-to-dipole) distances. As a morphological control, r was increased by re-processing the CdSe/CdS QR(675) to grow a 0.5 nm thick layer of ZnS, creating CdSe/CdS/ZnS QRs (denoted as QR(675*)). As a result, the QR(675*) have a slightly decreased aspect ratio, l/w=3.0±0.5 (1=26.6±3.8 nm, w=9.1±1.3 nm). Interestingly, this additional layer and larger distance had a significant effect on the BRET response, as seen in
(27) The dependence of QR aspect ratio and L on BRET response was next studied using both longer and shorter QRs. Referring to
(28) To further investigate the BR dependence on aspect ratio, CdSe/CdS QRs with smaller aspect ratio than both QR(675) and QR(628) were fabricated.
(29) The BRET efficiencies measured in
(30) TABLE-US-00001 TABLE 1 Bioluminscence properties of Ppy measured at pH 7.8 λ.sub.D De- Relative Activity.sup.1 (nm, Rise.sup.2 cay.sup.3 Flash Integration K.sub.m (μM) fwhm).sup.4 Enzyme (s) (min) height (15 min) LH.sub.2 Mg—ATP pH 7.8 Ppy WT 0.37 0.15 100 100 15 ± 2 160 ± 20 561 (79) 6xHis- 0.52 0.65 39 100 23 ± 3 163 ± 27 546 (77) Ppy GRTS
(31) By comparison, the R.sub.0 of 8.2 and 7.3 for QR(628) and QR(613), results in decreased efficiency ranges. The addition of the ZnS layers slightly changes the absorption profile of the QR, increasing R.sub.0 due to higher J-values, as represented by the dashed lines in
(32) In addition to spectral considerations, the BRET characteristics observed also indicate that QR morphology plays a critical role in influencing energy transfer parameters, as seen in
(33) This is illustrated in
(34) The present invention of quantum rods conjugated with the firefly P. pyralis luciferase variant PpyGRTS have high BRET ratios that are dependent upon enzyme loading, rod aspect ratio, and donor-to-acceptor distances. Moreover, and optimum loading of ˜5 was demonstrated, with QRs of aspect ratio of ˜3 that have rod-in-rod internal microstructure possessing the highest BRET efficiency of 44. Considering that such morphologies allow for a considerable red shift in QR emission, the present invention may be useful for optimizing NIR emission in future applications ranging from night vision capabilities to in-vivo imaging.
(35) The present invention may also be used to create BRET between PpyGRTS and near infrared (nIR) QD emitters, in which BR of 5˜6 are routine. The general QD functionalization process is outlined in
(36) Upon addition of the substrate LH.sub.2 to the PpyGRTS-QD(800) conjugates, we interestingly observed little to no visible light, suggesting the efficient energy transfer into the nIR.
(37) To better understand these results, the spectroscopic properties were compared, as seen in
(38) Nevertheless, BR of 5.0, and emission at 800 nm, this BRET nanosystem according to the present invention is easily observed via nIR imaging. As seen in
(39) Thus, the present invention provides a mechanism for directly attaching firefly P. pyralis luciferase variants (PpyGRTS) to nIR emitting QDs. Due to the high spectral overall provided by the nIR acting as the energy acceptor, high BRET efficiencies have been observed. The BRET ratios of 4-5 were measured using loading ratios of 0.5-2, making this one of the highest efficient BRET nanosystem. The resulting nIR emission is invisible to eye, but easily observed via night vision (i.e., nIR) detectors opening up this work to contribute to the fields of in-vivo imaging as well as broader scale signaling and sensing, each of which take advantage of the chemical substrate fuel.
Example
(40) Chemicals & Materials
(41) Cadmium oxide (CdO, 99.99%), trioctylphine (TOP, 90%), trioctylphine oxide (TOPO, 90%), octadecene (ODE, 90%), methylphosphonic acid (MPA, 98%), sulfur (S, 100 mesh), zinc acetate (ZnAc.sub.2, 99.99%), octylamine (99%), olelyamine (90%), L-histidine (His, >99.8%), sodium borohydrate (NaBH.sub.4, >96%), sodium tetraborate (99.5%), boric acid (>99.5%), toluene (≧99.5%), chloroform (>99.8%), methanol (>99.8%), acetone (99.5%) were purchased from Sigma Aldrich. Selenium (Se, 200 mesh 99.99%) was purchased from Alfa Aesar. Octadecylphosphonic acid (ODPA, 98%) and hexylphosphonic acid (HPA, 98%) were purchased from Strem Chemicals. Ultrapure water (18.2 MΩ) was provided from a Sartorius Stedim Arium 61316 reverse osmosis unit combined with an Arium 611DI polishing unit. The Mg-ATP (bacterial source) was purchased from Sigma-Aldrich, and restriction endonucleases from New England Biolabs (Beverly, Mass.). Firefly luciferin (LH.sub.2) was a generous gift from Promega (Madison, Wis.).
(42) Ppy Expression
(43) The Ppy WT was expressed as a GST-fusion protein and purified by affinity chromatography and stored. The plasmids for 6×His-Ppy GRTS was constructed by excising the corresponding genes for Ppy GRTS from the pGEX-6P-2 vector and ligating them into a modified pQE30 expression vector using previously described procedures. The His-tagged proteins were expressed, purified and stored using procedures described elsewhere. The found molecular masses (Da) of the proteins not previously reported were within the allowable experimental error (0.01%) of the calculated values (in parenthesis): 6×His-Ppy GRTS, 61 996 (62 002).
(44) Protein concentrations were determined with the Bio-Rad Protein Assay system using BSA as the standard. DNA sequencing to verify the ligations was performed at the W. M. Keck Biotechnology Laboratory at Yale University. Specific activity and steady state kinetics measurements were determined except that the final LH.sub.2 concentration was 0.3 mM and integration times were 15 min. Bioluminescence emission spectra were obtained using methods and equipment previously described. Mass spectral analyses were performed by tandem HPLC-electrospray ionization mass spectrometry (LC/ESIMS) using a ThermoFinnigan Surveyor HPLC system and a ThermoFinnigan LCQ Advantage mass spectrometer and previously developed conditions for protein mass determinations.
(45) Quantum Rod Synthesis:
(46) Quantum Rods with Dot-in-Dot (D/D) Morphology (CdSe/CdS(613))
(47) CdSe QDs were synthesized following traditional methods with slight modification. In a typical synthesis, CdO (0.06 g, 0.47 mmol), TOPO (3.00 g, 7.7 mmol), ODPA (0.28 g, 0.84 mmol) and 2 mL of ODE were mixed and heated to 150° C. under vacuum for 1 hour. Then, in an inert atmosphere, the reaction mixture was heated to 330° C. in order to dissolve CdO. When the solution changed from red-brown to clear and colorless, the temperature was increased to 360° C. Once the temperature stabilized an injection of Se (0.05 g, 0.63 mmol) and TOP (1.5 mL) was prepared in the glove box and injected into the reaction mixture. The reaction mixture was allowed to anneal for 10 minutes, then finally cooled to room temperature. In order to prevent solidification, a small amount of toluene was added at 60° C. The QDs were then purified free of excess ligands via multiple methanol extraction and precipitations. This process was repeated twice, and the final QD product was dried and dispersed in toluene. The approximate QD size and concentration was calculated as described below. The QD had PL emission centered at 602 nm, and the cores are denoted as CdSe(602).
(48) For rod growth, recent advances in methodology was applied (S8). For CdS shell deposition and rod growth on CdSe(602), the toluene was removed from the CdSe QDs via rotary evaporation and the QDs were dispersed in 5 mL of ODE and heated to 200° C. under inert atmosphere in a four-neck round-bottom flask. TOPO (0.5 g, 1.3 mmol) and oleylamine (0.75 mL, 2.3 mmol) were added to the mixture in order to limit CdSe QD growth. Then calculated amounts of cadmium precursor (0.2 M CdAc.sub.2 dissolved in octylamine) and sulfur precursor (0.2 M S dissolved in ODE) were injected sequentially, waiting 10 minutes between injections to allow for shell growth. After the final injection, CdSe/CdS(613) were annealed for 30 minutes. Finally the reaction mixture was cooled to room temperature and purified in the same manner as the CdSe QDs. The resulting CdSe/CdS(613) QR are characterized in
(49) Quantum Rods with Dot-in-Rod (D/R) Morphology (CdSe/CdS(628))
(50) CdSe QD cores were synthesized similarly to the CdSe QD described above with minor changes. The selenium precursor was injected at 370° C., then reaction mixture was taken off the heating mantle and cooled to room temperature as soon as a color change was observed, resulting in smaller QDs (d=3.9 nm). The QD cores were then purified as described above, and used for CdS rod shell was grown. In a typical experiment, a 25 mL four neck flask was filled with CdO (0.06 g, 0.47 mmol), TOPO (3.00 g, 7.7 mmol), ODPA (0.28 g, 0.84 mmol), HPA (0.08 g, 0.48 mmol) and 2 mL of ODE and heated to 150° C. under vacuum for 1 hour. Then in an inert atmosphere, the reaction was heated to 330° C. until the solution turned clear and colorless, then the temperature was increased to 370° C. Once the temperature stabilized a mixture of 8×10.sup.−8 moles of dried CdSe QDs and sulfur (0.12 g, 3.7 mmol) dissolved in 2.0 mL of TOP in the glove box and quickly injected into the reaction mixture and annealed for 10 min. The resulting CdSe/CdS(628) QR with dot-in-rod morphology are characterized in
(51) Quantum Rods with Rod-in-Rod (R/R) Morphology (CdSe/CdS(675)
(52) First, CdSe cores with rod morphology were synthesized. In a typical experiment, a mixture of CdO (0.06 g, 0.47 mmol), TOPO (3.00 g, 7.7 mmol), ODPA (0.24 g, 0.72 mmol), MPA (0.01 g, 0.10 mmol) and 2 mL of ODE in a four-neck round-bottom flask was heated to 150° C. under vacuum for one hour. Then the reaction mixture was placed under argon and the temperature was increased to 330° C. in order to dissolve CdO. When the solution changed from red-brown to clear and colorless, the temperature was raised to 365° C. Then 0.5 mL of TOP was injected. Once the temperature stabilized at 365° C., an injection of Se (0.05 g, 0.63 mmol) dissolved 1 mL of TOP prepared in the glove box, was swiftly injected into the reaction mixture and annealed for 10 minutes. Finally the reaction solution was cooled to room temperature. In order to prevent solidification, a small amount of toluene was added at 60° C. then cleaned and dispersed in toluene. The resulting CdSe rod cores had an aspect ratio of l/w=2.4±0.3. For growth of the CdS rod shell, CdO (0.03 g, 0.23 mmol), TOPO (3.00 g, 7.7 mmol), ODPA (0.14 g, 0.42 mmol), HPA (0.04 g, 0.24 mmol) and 2 mL of ODE were mixed and heated to 150° C. under vacuum for 1 hour. Then in an inert atmosphere, the reaction was heated to 330° C. until the solution turned clear and colorless, then the temperature was increased to 365° C. Once the temperature stabilized, a solution of 1×10.sup.−8 moles of dried CdSe QRs and sulfur (0.06 g, 1.9 mmol) dissolved in 2.0 mL of TOP in the glove box and quickly injected into the reaction flask. For the growth of CdS shell, the reaction mixture was annealed for 10 minutes after the injection. Next the QRs were cooled to room temperature and treated the same way as CdSe seeds. The resulting CdSe/CdS(675) QR with rod-in-rod morphology are characterized in
(53) ZnS Shell Growth on QRs.
(54) For each of the QR synthesized above, and additional thin layer of ZnS could also be grown following the SILAR approach. This was achieved at 200° C. in a mixture of QRs, TOPO (1.0 g) and ODE (5.0 mL). The zinc precursor (0.2 M ZnAc.sub.2 dissolved in octylamine) and sulfur precursor (0.2 M S dissolved in ODE) were injected slowly and sequentially, 100-200 μl injections at a time waiting 10 minutes between injections. After the final injection, the reaction mixture was allowed to anneal for 30 minutes. The total volume of precursor used was calculated based on the size of the QRs and the desired shell thickness. The resulting CdSe/CdS/ZnS QRs are characterized in
(55) Histidine-Mediated Phase Transfer
(56) In order to phase transfer the hydrophobic QRs into aqueous buffers a phase transfer technique was used. The organic ligands of the QR were directly exchanged with the small molecule L-histidine (His), rendering them hydrophilic, and both colloidally and optically stable. This histidine-mediated phase transfer method was achieved by adding a ˜5000-fold [His]:[QR] molar excess. Initially a histidine solution was prepared by dissolving histidine in a basic 3:1 MeOH/H.sub.2O solution. Then, ˜5000-fold excess of the histidine solution was added to cleaned QRs dispersed in chloroform and vortexed for 1 minute. This resulted in the QRs being transferred to the aqueous layer, to 10 mM borate buffer (pH 8.3). Excess organic ligands were back-extracted by addition of fresh chloroform, vortexing, and decanting of the organic solution. This extraction procedure was repeated at least four times. Then excess histidine molecules were removed by rinsing the hydrophilic QRs with 10 mM borate buffer using a 100 kDa molecular weight centrifugal filter (Millipore). Finally the QRs were dispersed in 10 mM borate buffer and refrigerated before use. The QR concentration was calculated as described below.
(57) PpyGRTS-QR Conjugation
(58) To construct the PpyGRTS-QR BRET nanosystems, the His-functionalized QRs were incubated with the histagged PpyGRTS in 10 mM borate buffer at loading ratios, L=[PpyGRTS]/[QR] on ice. Incubation was allowed to proceed for at least 15 minutes before BRET analysis.
(59) Instrumentation
(60) UV-Vis Spectrophotometry (UV-Vis).
(61) The UV-Vis measurements were collected on a Varian Cary100 Bio UV-Vis spectrophotometer between 200 and 900 nm. The instrument is equipped with an 8-cell automated holder with high precision Peltier heating controller.
(62) Photoluminescence (PL) and Bioluminescence
(63) The QR PL emission and Ppy bioluminescence was collected on a Fluoromax-4 photon counting spectrofluorometer (Horiba Jobin Yvon). The instrument is equipped with a 150 W xenon white light excitation source and computer-controlled monochromator. The detector is a R928P high sensitivity photon counting detector that is calibrated to emission wavelength. All PL emission and excitation spectra were collected using both wavelength correction of source intensity and detector sensitivity. The excitation wavelength for QR quantum yield calculations was 400 nm using 3 nm excitation and emission slits unless otherwise noted. Bioluminescence spectra were collected with the excitation source blocked.
(64) Transmission Electron Microscopy (TEM)
(65) TEM measurements were performed on a JEOL 2000EX instrument operated at 100 kV with a tungsten filament (SUNY-ESF, N.C. Brown Center for Ultrastructure Studies). Negative staining was achieved using phosphotungstic acid. Particle size and aspect ratio were analyzed manually with statistical analysis per-formed using ImageJ software on populations of at least 100 counts.
(66) BRET Measurement and Analysis
(67) In a typical BRET experiment, a mixture of 100 μL of 91 μM LH.sub.2 (firefly luciferin) and 30 μL of 8.66 mM Mg-ATP in 25 mM gly-gly buffer (pH 7.8) is quickly added to the Ppy-QR conjugate solution ([QR]=230-700 nM) in a 96-well plate and bioluminescence emission is immediately collected. The bioluminescence and BRET were collected on a Varian Cary-Eclipse spectrophotometer in bioluminescence/chemiluminescence mode using a 96-well plate reading accessory. White 96-well plates were employed, with volumes ranging from 50-200 mL. Bioluminescence spectra were collected every 15 seconds for 7.5 minutes. The instrument was corrected for detector sensitivity by comparison of fluorescence standard emission intensities (500-800 nm) with the corrected detector on the Fluoromax-4 spectrophotometer (see above). The presented BRET results are the average of the first five spectra collected over 1.5 min after addition of LH.sub.2. Control experiments showed that the BRET ratio did not change over the course of the typical BRET decay. Additionally, control experiments showed that BRET efficiency was not influenced greatly by [LH.sub.2], but overall signals improved at high excess of LH.sub.2. Finally, the BRET efficiencies of the systems were calculated as BRET ratio (BR), which is defined as the ratio of peak area of the acceptor and donor emission respectively. Peak area was calculated by spectral deconvolution of each spectrum using the data analysis package in Igor Pro (Wavemetrics Inc.).
(68) Calculations
(69) QD and QR Concentration
(70) The concentrations of the QD cores were calculated based on UV-vis optical absorption measurements of the QD first band edge absorption (1s-1s) intensity using QD size dependent optical extinction coefficients (ε). The concentration of particles with QR morphologies was calculated based on UV-vis optical absorption at 350 nm and extinction coefficients (ε.sub.QR) based on the average volume of the QRs. The final concentrations were obtained using the Beer-Lambert equation, Abs=εbc; where c is the estimated extinction coefficient (M.sup.−1 cm-1), b is the path length, and c is concentration. A tabulation of the calculated c values is given in Table 2 below.
(71) TABLE-US-00002 TABLE 2 Morphological and optical properties for the quantum rods (QR) of the present invention Morphology Optical Properties Name Type Class.sup.A l/w l(nm) W(nm) QY(%) λ.sub.Abs(nm).sup.B εA(M.sup.−1cm.sup.−1).sup.C QR(675) CdSe/CdS (R/R) 3.1 ± 0.5 24.8 ± 3.6 8.2 ± 1.3 23 632 791559.8 QR(675*) CdSe/CdS/ZnS (R/R) 3 ± 0.5 26.6 ± 3.8 9.1 ± 1.3 14 638 940895.7 QR(628) CdSe/CdS (D/R) 8.7 ± 1.6 50.1 ± 5.5 5.9 ± 0.8 33 603 357503.9 QR(628*) CdSe/CdS/ZnS (D/R) 7.4 ± 1.5 52.1 ± 5.8 7.2 ± 1.1 21 610 429857 QR(613) CdSe/CdS (D/D) 1.8 ± 0.2 9.1 ± 0.9 5 ± 0.4 19 600 330942.4 QR(613*) CdSe/CdS/ZnS (D/D) 1.9 ± 0.3 10.3 ± 1.3 5.3 ± 0.5 18 603 357503.9
(72) Quantum Yield (QY)
(73) The QR photoluminescence quantum yields (QY) were calculated based on comparison to a reference dye using standard methods (equation 1) (S13):
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where QY.sub.R is the reference dye quantum yield (Rhodamine 6G=95%), Abs.sub.R and Abs.sub.QD are the optical absorption at specific excitation for the reference dye and QR samples respectively. Here, careful attention was paid to prepare samples with optical absorption below 0.10 in order to limit inner filter effects. PL.sub.R and PL.sub.QD correspond to the total area of the PL emission after wavelength dependent calibration of both the excitation source, and photoluminescence detector, as well as after PL spectra baseline correction.
(75) Förster Resonance Energy Transfer (FRET) Calculations
(76) In this study, the bioluminescence resonance energy transfer (BRET) constants were calculated in the identical manner to FRET. In FRET, the Förster distance (R.sub.0), in units of angstroms, is calculated using equation 2:
R.sub.0=9.78×10.sup.3((κ.sup.2n.sup.−4Q.sub.DJ(λ)).sup.1/6 (2)
where n refractive index of the medium (n=1.33), κ is the dipole orientation factor (κ=⅔), Q.sub.D is the donor quantum yield QY(GRTS)≈32%, and J(λ) is the spectral overlap integral. The J(λ) value can be calculated using equation 3:
J(λ)=∫F.sub.D(λ)ε.sub.A(λ)λ.sup.4dλ, (3)
where λ is the defined wavelength of the donor-acceptor spectral overlap, and F.sub.D(λ) is the integrated donor emission with area normalized to unity, and ε.sub.A(λ) represents the acceptor extinction coefficient at the particular wavelength. When ε.sub.A(λ) is in units of M.sup.−1 cm.sup.−1, and l is in units of cm, the units for J(λ) are M.sup.−1 cm.sup.3, and equation 2 is relevant.
(77) Using the R.sub.0 values calculated above, the FRET efficiency, E, was calculated using equation 4:
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where F.sub.DA is donor fluorescence in the presence of acceptor, and F.sub.D is fluorescence of the donor without acceptor. A tabulation of the calculated BRET constants is shown in Table 3 below:
(79) TABLE-US-00003 TABLE 3 Calculated BRET constants for the Ppy-QR nanosystems PPy GRTS.sup.A Name Type J (M.sup.−1 cm.sup.3).sup.B R.sub.0 (mn).sup.C QR(675) CdSe/CdS (R/R) 1.19 × 10.sup.−11 9.4 QR(675*) CdSe/CdS/ZnS (R/R) 1.65 × 10.sup.−11 10.0 QR(628) CdSe/CdS (D/R) 5.26 × 10.sup.−12 8.2 QR(628*) CdSe/CdS/ZnS (D/R) 6.21 × 10.sup.−12 8.5 QR(613) CdSe/CdS (D/D) 2.49 × 10.sup.−12 7.3 QR(613*) CdSe/CdS/ZnS (D/D) 2.77 × 10.sup.−12 7.4
where .sup.APPy GRTS, QY=32%, λ.sub.D=546 nm; .sup.B Calculated using equation 3; and .sup.CCalculated using equation 2.