Alkoxybenzo-five-membered (six-membered) heterocyclic amine compound and pharmaceutical use thereof

Abstract

Alkoxybenzeno five- or six-member heterocyclic amines compounds, their pharmaceutically acceptable salts, and pharmaceutical compositions are used as the active ingredients, and their application in drugs which can prevent and cure diseases caused by abnormal increasing of SM. These diseases caused by abnormal increasing of SM include atherosclerosis, type II diabetes, fatty liver, obesity, metabolic syndromes, enteritis and other inflammatory diseases.

Claims

1. Benzeno five member heterocyclic amine compounds represented by formula (I), and their pharmaceutically acceptable salts, wherein formula (I) is ##STR00058## wherein, X and Y are independently selected from oxygen, nitrogen, and sulfur; and X and Y are not both oxygen or sulfur; R.sub.1 is chosen from benzene ring or 5-6 membered heterocycle, wherein the heteroatoms in the heterocycle are one to three atoms optionally selected from oxygen, nitrogen and sulfur; R.sub.2 is chosen from one of hydrogen, methyl, ethyl, and propyl; R.sub.3 is chosen from phenyl methylene and substituted phenyl methylene, wherein the substituents in phenyl are one to three groups independently selected from halogen, —CN, C.sub.1-C.sub.3 alkyl, C.sub.1-C.sub.3 alkoxyl, C.sub.1-C.sub.3 haloalkyl, C.sub.1-C.sub.3 haloalkoxyl, wherein each of haloalkyl or haloalkoxyl independently contains one to three halogen atoms, the halogen atom is selected from F, Cl and Br, wherein the compounds comprise the following structures: ##STR00059##

2. The heterocyclic amine compounds according to claim 1, further comprises the following structures: ##STR00060## ##STR00061## ##STR00062## ##STR00063## ##STR00064## ##STR00065## ##STR00066## ##STR00067## ##STR00068##

3. The heterocyclic amine compounds according to claim 1, wherein the pharmaceutically acceptable salts comprise hydrochloride salts, hydrogen bromide salts, tartrate and methanesulfonate.

4. A pharmaceutical composition with medicinally acceptable carriers comprising the heterocyclic amine compounds and their pharmaceutically acceptable salts according to claim 1.

5. A method of treatment of abnormal increase of sphingomyelin level, comprising treating a patient of said disease with the pharmaceutical composition with medicinally acceptable carriers, the heterocyclic amine compounds and their pharmaceutically acceptable salts according to claim 1.

6. The heterocyclic amine compounds according to claim 1, wherein the pharmaceutically acceptable salts comprise solvates of the heterocyclic amine compounds, wherein the solvates comprise water, ethanol, and methanol.

7. A method of treatment of diseases caused by abnormal increase of sphingomyelin level, comprising treating a patient of said diseases with drugs prepared from the pharmaceutical composition with medicinally acceptable carriers, the heterocyclic amine compounds and their pharmaceutically acceptable salts according to claim 5.

8. The method according to claim 7, wherein the diseases are selected from atherosclerosis, type II diabetes, fatty liver, obesity, metabolic syndromes, enteritis and other inflammatory diseases.

9. The heterocyclic amine compounds according to claim 1, wherein the compounds comprise the following structures: ##STR00069## wherein X, Y, R.sub.2 and R.sub.3 are defined as above in claim 1.

10. The pharmaceutical composition with medicinally acceptable carriers comprising the heterocyclic amine compounds and their pharmaceutically acceptable salts according to claim 4, wherein the pharmaceutically acceptable salts comprise solvates of the heterocyclic amine compounds, wherein the solvates comprise water, ethanol, and methanol.

Description

DETAILED DESCRIPTION

Application Case 1: Preparation of 4-((2-ethylbenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-1)

1. Synthesis of 2-(Benzyloxy)-6-fluorobenzonitrile (Compound 4)

(1) ##STR00016##

(2) To a mixture of 10.00 g (73.0 mmol, 2.0 eq) K.sub.2CO.sub.3, 200 mg KI and 5.00 g 2-fluoro-6-hydroxybenzonitrile (36.5 mmol, 1.0 eq) in 100 ml acetonitrile, then 6.55 g (38.3 mmol, 1.05 eq) benzyl bromide was added to the mixture, and the whole was stirred at room temperature for 12 h. After the reaction was complete, most of the solution was removed under reduced pressure, water was added to the residue and extracted with EA, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated. The residue was purified with column chromatography (PE/EA=15:1) to give compound 4, 8.0 g white solid, yield 96%.

(3) The structure is confirmed correct and data are as follow: MS (ESI) (m/z): 228.0 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 7.73-7.62 (m, 1H), 7.43 (d, J=7.0 Hz, 2H), 7.38 (t, J=7.4 Hz, 2H), 7.32 (d, J=7.1 Hz, 1H), 7.16 (d, J=8.7 Hz, 1H), 7.03 (t, J=8.8 Hz, 1H), 5.27 (s, 2H).

2. Synthesis of 4-(Benzyloxy)benzo[d]isoxazol-3-amine (Compound 6)

(4) ##STR00017##

(5) To a solution of 4.0 g acetohydroxamic acid (53.3 mmol, 1.5 eq) in 150 ml dry DMF, then add 6.0 g t-BuOK (53.3 mmol, 1.5 eq) and stir at rt for 0.5 h under nitrogen. Then, 8.0 g 2-(Benzyloxy)-6-fluorobenzonitrile (35.2 mmol, 1.0 eq) was added in batches. After an additional 6 h of stirring at rt, most of the solution was removed under reduced pressure, water was added to the residue and extracted with EA, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated. The crude product was purified by recrystallization with a mixed solvent of DCM and PE to give 2.0 g compound 6, yield 24%.

(6) The structure is confirmed correct and data are as follow: MS(ESI) (m/z): 241.0 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 7.50 (d, J=6.8 Hz, 2H), 7.42-7.34 (m, 3H), 7.34-7.28 (m, 1H), 6.96 (d, J=8.4 Hz, 1H), 6.76 (d, J=8.0 Hz, 1H), 5.86 (s, 2H), 5.29 (s, 2H).

3. Synthesis of 4-(Benzyloxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Compound 8)

(7) ##STR00018##

(8) A mixture of 3.0 g 4-(benzyloxy)benzo[d]isoxazol-3-amine (12.5 mmol, 1.0 eq) and 3.0 g 3-bromopyridine (18.75 mmol, 1.5 eq), 1.14 g Pd.sub.2(dba).sub.3(1.25 mmol, 0.1 eq), 1.44 g Xantphos (2.50 mmol, 0.2 eq), and 3.45 g anhydrous potassium carbonate (25.0 mmol, 2.0 eq) in 50 ml dioxane. After nitrogen substitution three times, heat to 125° C. and reflux for 12 h under nitrogen protection. After the reaction was complete, most of the solution was removed under reduced pressure, water was added to the residue and extracted with EA, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated. The residue was purified with column chromatography (PE/EA=3:2) to give compound 8, 3.1 g beige solid, yield 78%.

(9) The structure is confirmed correct and data are as follow: MS(ESI) (m/z): 318.0 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.73 (d, J=2.8 Hz, 1H), 8.28 (s, 1H), 8.18 (dd, J=4.7, 1.4 Hz, 1H), 8.09-8.01 (m, 1H), 7.54 (d, J=7.5 Hz, 2H), 7.47 (t, J=8.2 Hz, 1H), 7.43-7.34 (m, 3H), 7.31 (t, J=7.2 Hz, 1H), 7.11 (d, J=8.4 Hz, 1H), 6.85 (d, J=8.0 Hz, 1H), 5.41 (s, 2H).

4. Synthesis of 3-(pyridin-3-ylamino)benzo[d]isoxazol-4-ol (Compound 9)

(10) ##STR00019##

(11) A mixture of 3.1 g 4-(Benzyloxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (9.8 mmol, 1.0 eq) in 20 ml 40% HBr aqueous solution and 20 ml HOAc was heated to 65° C. and reacted for 12 h. After the reaction was complete, most of the solution was removed under reduced pressure, then neutralize with saturated sodium carbonate aqueous solution to pH=8, add appropriate EA to extract, then obtain a suspension, filter to obtain a part of gray solid product, the mother liquor continues to extract with EA, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated. The residue was purified with column chromatography (DCM/MeOH=20:1) to give compound 9, 1.4 g gray solid, yield 63%.

(12) The structure is confirmed correct and data are as follow: MS (ESI) (m/z): 228.0 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 11.20 (s, 1H), 8.90 (d, J=2.7 Hz, 1H), 8.35 (s, 1H), 8.20-8.13 (m, 2H), 7.40-7.32 (m, 2H), 6.97 (d, J=8.3 Hz, 1H), 6.65 (d, J=7.8 Hz, 1H).

5. Synthesis of 2-ethylbenzyl Bromide (Compound 11)

(13) ##STR00020##

(14) Dissolve 250 mg 2-ethylbenzyl alcohol (1.84 mmol, 1.0 eq) in 10 mL anhydrous ether, cool to 0° C. with an ice water bath, add 191 mg of phosphorus tribromide (0.72 mmol, 0.5 eq). After reacting at 0° C. for 15 min, the ice-water bath was removed, wait for the temperature to rise to room temperature and react for 2 h. Then the mixture was cooled to 0° C. with ice-water bath, water was added to the residue and extracted with EA, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated to give compound 11, 250 mg oily substance, yield 89%. The crude product was used without purification in the next step directly.

6. Synthesis of 4-((2-ethylbenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-1)

(15) ##STR00021##

(16) 50 mg 3-(pyridin-3-ylamino)-4-hydroxybenzo[d]isoxazole (0.22 mmol, 1.0 eq), 44 mg 2-ethylbenzyl bromide (0.22 mmol, 1.0 eq) and 61 mg anhydrous potassium carbonate (0.44 mmol, 2.0 eq) was mixed, and 5 mL acetone was added, then the mixture was reacted at room temperature for 3 h, water was added, extracted with EA, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated. The residue was purified with column chromatography (PE/EA=2:1) to give compound I-1, 30 mg white solid, yield 39%.

(17) The structure is confirmed correct and data are as follow: MS (ESI) (m/z): 346.0 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.64 (d, J=2.7 Hz, 1H), 8.22 (s, 1H), 8.15 (d, J=4.6 Hz, 1H), 8.00 (dt, J=8.6, 2.0 Hz, 1H), 7.48 (t, J=7.8 Hz, 2H), 7.35 (dd, J=8.4, 4.7 Hz, 1H), 7.28-7.21 (m, 2H), 7.22-7.15 (m, 1H), 7.11 (d, J=8.4 Hz, 1H), 6.89 (d, J=8.0 Hz, 1H), 5.44 (s, 2H), 2.73 (t, J=7.5 Hz, 2H), 1.11 (t, J=7.5 Hz, 3H).

Application Case 2: Synthesis of Scheme I-2, I-4˜I-27

(18) TABLE-US-00001 Scheme I R Yield (%) I-2  2-Cl,5F 59 I-4  2-F 52 I-5  3-F 58 I-6  4-F 70 I-7  2-Cl 55 I-8  3-Cl 60 I-9  4-Cl 65 I-10 2-Me 50 I-11 3-Me 56 I-12 4-Me 65 I-13 2-CF.sub.3 40 I-14 3-CF.sub.3 60 I-15 4-CF.sub.3 65 I-16 2-OMe 38 I-17 3-OMe 49 I-18 4-OMe 50 I-19 2-OCF.sub.3 43 I-20 3-OCF.sub.3 60 I-21 4-OCF.sub.3 66 I-22 2-CN 45 I-23 3-CN 50 I-24 4-CN 70 I-25 2-Me,6-Me 35 I-26 2-Cl,6-Cl 38 I-27 2-F,6-F 40

(19) Referring to reaction conditions of the fifth step of synthesizing Scheme I-1 in Application case 1, reaction of 3-(Pyridin-3-ylamino)benzo[d]isoxazol-4-ol with the corresponding substituted benzyl bromide gives the corresponding target compounds Scheme I-2 and I-4 to I-27, that is: 4-((2-Chloro-5-fluorobenzyl)oxy)-N-(pyridin-3-yl)benzo[d]-isoxazol-3-amine (Scheme I-2); 4-((2-fluorobenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-4); 4-((3-fluorobenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-5); 4-((4-fluorobenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-6); 4-((2-chlorobenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-7); 4-((3-chlorobenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-8); 4-((4-chlorobenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-9); 4-((2-methylbenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-10); 4-((3-methylbenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-11); 4-((4-methylbenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-12); 4-((2-Trifluoromethylbenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazole-3-amine (Scheme I-13); 4-((3-Trifluoromethylbenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazole-3-amine (Scheme I-14); 4-((4-Trifluoromethylbenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazole-3-amine (Scheme I-15); 4-((2-Methoxybenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-16); 4-((3-Methoxybenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-17); 4-((4-Methoxybenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-18); 4-((2-Trifluoromethoxybenzyl)oxy)-N-(pyridin-3-yl)benzo[d]-isoxazol-3-amine (Scheme I-19); 4-((3-Trifluoromethoxybenzyl)oxy)-N-(pyridin-3-yl)benzo[d]-isoxazol-3-amine (Scheme I-20); 4-((4-Trifluoromethoxybenzyl)oxy)-N-(pyridin-3-yl)benzo[d]-isoxazol-3-amine (Scheme I-21); 4-((2-Cyanobenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-22); 4-((3-Cyanobenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-23); 4-((4-Cyanobenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-24); 4-((2,6-Dimethylbenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-25); 4-((2,6-Dichlorobenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-26); 4-((2,6-Difluorobenzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (scheme I-27).

(20) The structures were confirmed correct and data are as follow:

(21) Scheme I-2 MS(ESI) (m/z): 370.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.72 (d, J=2.7 Hz, 1H), 8.32 (s, 1H), 8.18 (dd, J=4.7, 1.5 Hz, 1H), 8.05 (d, J=8.4 Hz, 1H), 7.64-7.51 (m, 3H), 7.38 (dd, J=8.4, 4.7 Hz, 1H), 7.29 (dt, J=8.7, 4.3 Hz, 1H), 7.20 (d, J=8.4 Hz, 1H), 6.90 (d, J=8.0 Hz, 1H), 5.46 (s, 2H).

(22) Scheme I-4 MS(ESI) (m/z): 336.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.67 (d, J=2.7 Hz, 1H), 8.20 (s, 1H), 8.15 (d, J=4.6 Hz, 1H), 8.00 (d, J=8.5 Hz, 1H), 7.60 (t, J=7.6 Hz, 1H), 7.48 (t, J=8.2 Hz, 1H), 7.35 (td, J=9.2, 8.7, 5.6 Hz, 2H), 7.21 (dt, J=15.3, 8.6 Hz, 2H), 7.12 (d, J=8.6 Hz, 1H), 6.89 (d, J=8.1 Hz, 1H), 5.44 (s, 2H).

(23) Scheme I-5 MS(ESI) (m/z): 336.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.75 (d, J=2.7 Hz, 1H), 8.34 (s, 1H), 8.16 (dd, J=4.7, 1.5 Hz, 1H), 8.06 (ddd, J=8.4, 3.0, 1.5 Hz, 1H), 7.48 (d, J=8.2 Hz, 1H), 7.46-7.39 (m, 2H), 7.36 (dd, J=8.7, 3.9 Hz, 2H), 7.12 (d, J=8.4 Hz, 2H), 6.82 (d, J=8.0 Hz, 1H), 5.41 (s, 2H).

(24) Scheme I-6 MS(ESI) (m/z): 336.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.71 (s, 1H), 8.25 (s, 1H), 8.14 (d, J=4.6 Hz, 1H), 8.02 (d, J=8.4 Hz, 1H), 7.65-7.51 (m, 2H), 7.43 (t, J=8.2 Hz, 1H), 7.33 (dd, J=8.3, 4.7 Hz, 1H), 7.18 (t, J=8.7 Hz, 2H), 7.08 (d, J=8.4 Hz, 1H), 6.81 (d, J=8.1 Hz, 1H), 5.35 (s, 2H).

(25) Scheme I-7 MS(ESI) (m/z): 336.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.67 (s, 1H), 8.23 (s, 1H), 8.15 (s, 1H), 8.01 (d, J=8.4 Hz, 1H), 7.60 (s, 1H), 7.49 (q, J=8.9, 7.5 Hz, 2H), 7.34 (s, 3H), 7.14 (d, J=8.4 Hz, 1H), 6.85 (d, J=7.9 Hz, 1H), 5.46 (s, 2H).

(26) Scheme I-8 MS(ESI) (m/z): 352.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.77 (d, J=2.6 Hz, 1H), 8.33 (s, 1H), 8.21-8.14 (m, 1H), 8.11-8.03 (m, 1H), 7.67 (s, 1H), 7.48 (dd, J=9.4, 7.2 Hz, 2H), 7.37 (td, J=10.2, 8.7, 5.8 Hz, 3H), 7.13 (d, J=8.4 Hz, 1H), 6.83 (d, J=8.0 Hz, 1H), 5.41 (s, 2H).

(27) Scheme I-9 MS(ESI) (m/z): 352.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.77 (d, J=2.7 Hz, 1H), 8.30 (s, 1H), 8.19 (d, J=4.6 Hz, 1H), 8.11-8.02 (m, 1H), 7.58 (d, J=8.1 Hz, 2H), 7.45 (dd, J=8.1, 6.2 Hz, 3H), 7.37 (dd, J=8.4, 4.7 Hz, 1H), 7.12 (d, J=8.4 Hz, 1H), 6.83 (d, J=8.0 Hz, 1H), 5.41 (s, 2H).

(28) Scheme I-10 MS(ESI) (m/z): 332.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.54 (s, 1H), 8.17-7.98 (m, 2H), 7.89 (d, J=8.4 Hz, 1H), 7.34 (d, J=8.0 Hz, 2H), 7.29-7.17 (m, 1H), 7.09 (s, 3H), 6.99 (d, J=8.4 Hz, 1H), 6.75 (d, J=8.0 Hz, 1H), 5.28 (s, 2H), 2.24 (s, 3H).

(29) Scheme I-11 MS(ESI) (m/z): 332.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.71 (d, J=2.7 Hz, 1H), 8.28 (s, 1H), 8.16 (d, J=4.6 Hz, 1H), 8.03 (d, J=8.4 Hz, 1H), 7.46 (t, J=8.2 Hz, 1H), 7.35 (q, J=5.3 Hz, 2H), 7.30 (d, J=7.7 Hz, 1H), 7.25 (t, J=7.5 Hz, 1H), 7.10 (d, J=8.1 Hz, 2H), 6.82 (d, J=8.0 Hz, 1H), 5.35 (s, 2H), 2.26 (s, 3H).

(30) Scheme I-12 MS(ESI) (m/z): 332.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.73 (s, 1H), 8.27 (s, 1H), 8.17 (s, 1H), 8.05 (d, J=8.3 Hz, 1H), 7.44 (t, J=8.2 Hz, 3H), 7.37 (d, J=7.5 Hz, 1H), 7.17 (d, J=7.6 Hz, 2H), 7.10 (d, J=8.4 Hz, 1H), 6.83 (d, J=7.9 Hz, 1H), 5.36 (s, 2H), 2.25 (s, 3H).

(31) Scheme I-13 MS(ESI) (m/z): 386.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.66 (d, J=2.8 Hz, 1H), 8.19 (s, 1H), 8.15 (d, J=4.6 Hz, 1H), 8.08-7.95 (m, 1H), 7.79 (d, J=7.9 Hz, 2H), 7.69 (t, J=7.7 Hz, 1H), 7.52 (dt, J=29.6, 7.9 Hz, 2H), 7.34 (dd, J=8.5, 4.7 Hz, 1H), 7.15 (d, J=8.4 Hz, 1H), 6.76 (d, J=8.0 Hz, 1H), 5.55 (s, 2H).

(32) Scheme I-14 MS(ESI) (m/z): 386.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.73 (d, J=2.7 Hz, 1H), 8.37 (s, 1H), 8.19-8.12 (m, 1H), 8.08-8.01 (m, 1H), 7.97 (s, 1H), 7.82 (d, J=7.6 Hz, 1H), 7.66 (d, J=7.9 Hz, 1H), 7.60 (t, J=7.7 Hz, 1H), 7.48 (t, J=8.3 Hz, 1H), 7.34 (dd, J=8.4, 4.7 Hz, 1H), 7.12 (d, J=8.5 Hz, 1H), 6.85 (d, J=8.1 Hz, 1H), 5.48 (s, 2H).

(33) Scheme I-15 MS(ESI) (m/z): 386.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.79 (d, J=2.7 Hz, 1H), 8.40 (s, 1H), 8.17 (d, J=4.7 Hz, 1H), 8.08 (d, J=8.5 Hz, 1H), 7.74 (s, 4H), 7.46 (t, J=8.2 Hz, 1H), 7.36 (dd, J=8.4, 4.8 Hz, 1H), 7.12 (d, J=8.5 Hz, 1H), 6.79 (d, J=8.1 Hz, 1H), 5.52 (s, 2H).

(34) Scheme I-16 MS(ESI) (m/z): 348.0 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.66 (d, J=2.7 Hz, 1H), 8.18 (d, J=7.1 Hz, 2H), 8.01 (d, J=8.0 Hz, 1H), 7.53-7.43 (m, 2H), 7.39-7.30 (m, 2H), 7.10 (dd, J=19.3, 8.3 Hz, 2H), 6.94 (t, J=7.3 Hz, 1H), 6.85 (d, J=8.0 Hz, 1H), 5.38 (s, 2H), 3.81 (s, 3H).

(35) Scheme I-17 MS(ESI) (m/z): 348.0 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.74 (d, J=2.7 Hz, 1H), 8.31 (s, 1H), 8.17 (d, J=4.7 Hz, 1H), 8.06 (d, J=8.1 Hz, 1H), 7.46 (t, J=8.2 Hz, 1H), 7.40-7.32 (m, 1H), 7.28 (t, J=7.9 Hz, 1H), 7.11 (dd, J=14.6, 6.4 Hz, 3H), 6.85 (t, J=8.9 Hz, 2H), 5.37 (s, 2H), 3.69 (s, 3H).

(36) Scheme I-18 MS(ESI) (m/z): 348.0 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.71 (d, J=2.7 Hz, 1H), 8.22 (s, 1H), 8.17 (d, J=4.7 Hz, 1H), 8.07-8.01 (m, 1H), 7.47 (dd, J=12.7, 8.1 Hz, 3H), 7.36 (dd, J=8.4, 4.7 Hz, 1H), 7.09 (d, J=8.4 Hz, 1H), 6.92 (d, J=8.3 Hz, 2H), 6.87 (d, J=8.0 Hz, 1H), 5.32 (s, 2H), 3.70 (s, 3H).

(37) Scheme I-19 MS(ESI) (m/z): 402.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.68 (d, J=2.7 Hz, 1H), 8.21 (s, 1H), 8.17 (d, J=4.7 Hz, 1H), 8.02 (d, J=7.9 Hz, 1H), 7.73 (d, J=7.6 Hz, 1H), 7.55-7.47 (m, 2H), 7.44 (d, J=7.6 Hz, 2H), 7.36 (dd, J=8.4, 4.8 Hz, 1H), 7.16 (d, J=8.4 Hz, 1H), 6.85 (d, J=8.0 Hz, 1H), 5.48 (s, 2H).

(38) Scheme I-20 MS(ESI) (m/z): 402.0 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.75 (d, J=2.8 Hz, 1H), 8.33 (s, 1H), 8.17 (d, J=4.6 Hz, 1H), 8.09-8.01 (m, 1H), 7.57 (d, J=10.4 Hz, 2H), 7.50 (dt, J=12.3, 8.1 Hz, 2H), 7.39-7.27 (m, 2H), 7.13 (d, J=8.4 Hz, 1H), 6.86 (d, J=8.0 Hz, 1H), 5.45 (s, 2H).

(39) Scheme I-21 MS(ESI) (m/z): 402.0 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.76 (d, J=2.7 Hz, 1H), 8.31 (s, 1H), 8.18 (d, J=4.6 Hz, 1H), 8.05 (d, J=8.5 Hz, 1H), 7.67 (d, J=8.3 Hz, 2H), 7.48 (t, J=8.2 Hz, 1H), 7.38 (d, J=8.1 Hz, 3H), 7.12 (d, J=8.4 Hz, 1H), 6.84 (d, J=7.9 Hz, 1H), 5.44 (s, 2H).

(40) Scheme I-22 MS(ESI) (m/z): 343.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.71 (d, J=2.6 Hz, 1H), 8.17 (d, J=4.7 Hz, 2H), 8.04 (ddd, J=8.3, 2.8, 1.4 Hz, 1H), 7.96-7.88 (m, 1H), 7.83 (d, J=7.8 Hz, 1H), 7.79-7.71 (m, 1H), 7.61-7.47 (m, 2H), 7.36 (dd, J=8.4, 4.7 Hz, 1H), 7.17 (d, J=8.4 Hz, 1H), 6.95 (d, J=8.0 Hz, 1H), 5.60 (s, 2H).

(41) Scheme I-23 MS(ESI) (m/z): 343.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.74 (s, 1H), 8.33 (s, 1H), 8.14 (d, J=4.6 Hz, 1H), 8.04 (d, J=5.3 Hz, 2H), 7.85 (d, J=7.9 Hz, 1H), 7.76 (d, J=7.6 Hz, 1H), 7.58 (d, J=7.7 Hz, 1H), 7.45 (t, J=8.2 Hz, 1H), 7.39-7.29 (m, 1H), 7.11 (d, J=8.4 Hz, 1H), 6.80 (d, J=8.0 Hz, 1H), 5.42 (s, 2H).

(42) Scheme I-24 MS(ESI) (m/z): 343.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.78 (s, 1H), 8.37 (s, 1H), 8.21-8.10 (m, 1H), 8.06 (d, J=8.4 Hz, 1H), 7.83 (d, J=7.8 Hz, 2H), 7.69 (d, J=7.9 Hz, 2H), 7.44 (t, J=8.2 Hz, 1H), 7.35 (t, J=6.7 Hz, 1H), 7.11 (d, J=8.4 Hz, 1H), 6.76 (d, J=8.0 Hz, 1H), 5.49 (s, 2H).

(43) Scheme I-25 MS(ESI) (m/z): 346.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.27 (d, J=2.7 Hz, 1H), 8.11 (d, J=4.6 Hz, 1H), 7.79 (d, J=5.5 Hz, 2H), 7.56 (t, J=8.2 Hz, 1H), 7.29 (dd, J=8.5, 4.7 Hz, 1H), 7.16 (dd, J=7.8, 5.8 Hz, 2H), 7.08 (d, J=7.5 Hz, 2H), 7.01 (d, J=8.0 Hz, 1H), 5.34 (s, 2H), 2.36 (s, 6H).

(44) Scheme I-26 MS(ESI) (m/z): 386.0 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.46 (d, J=2.7 Hz, 1H), 8.15 (d, J=4.7 Hz, 1H), 7.94 (s, 1H), 7.89 (d, J=9.5 Hz, 1H), 7.60 (d, J=7.6 Hz, 3H), 7.50 (dd, J=8.9, 7.2 Hz, 1H), 7.34 (dd, J=8.4, 4.6 Hz, 1H), 7.23 (d, J=8.4 Hz, 1H), 7.13 (d, J=8.0 Hz, 1H), 5.54 (s, 2H).

(45) Scheme I-27 MS(ESI) (m/z): 354.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.60 (s, 1H), 8.19 (d, J=4.3 Hz, 1H), 8.11 (s, 1H), 7.97 (d, J=8.2 Hz, 1H), 7.66-7.48 (m, 2H), 7.37 (dd, J=8.3, 4.4 Hz, 1H), 7.26-7.14 (m, 3H), 7.07 (d, J=7.9 Hz, 1H), 5.49 (s, 2H).

Application Case 3: Synthesis of 4-((5-chloro-2-(trifluoromethoxy)benzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-3)

1. Synthesis of 5-chloro-2-(trifluoromethoxy)benzaldehyde (Compound 13)

(46) ##STR00024##

(47) Dissolve 1.0 g 1-chloro-4-(trifluoromethoxy)benzene (5.1 mmol, 1.0 eq) in 20 mL anhydrous tetrahydrofuran, protect with nitrogen, cool to −80° C., and add 3.1 mL 2M LDA (6.1 mmol, 1.2 eq) dropwise. After 15 minutes of dripping, keep at −80° C. for 20 min, add 0.47 mL DMF, slowly warm to −50° C. for 40 min, add 1.22 g acetic acid (20.4 mmol, 4.0 eq) to quench the reaction. Then water was added to the residue and extracted with EA, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated. The residue was purified with column chromatography (PE/EA=20:1) to give compound 13, 800 mg light yellow oily substance, yield 70%.

(48) The structure is confirmed correct and data are as follow: MS (ESI) (m/z): 225.0 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 10.14 (s, 1H), 7.91 (d, J=2.7 Hz, 1H), 7.88 (dd, J=8.8, 2.6 Hz, 1H), 7.60 (dd, J=8.8, 1.7 Hz, 1H).

2. Synthesis of 5-chloro-2-(trifluoromethoxy)phenyl)methanol (Compound 14)

(49) ##STR00025##

(50) Add 800 mg 5-chloro-2-(trifluoromethoxy)benzaldehyde (3.57 mmol, 1.0 eq) and 10 mL ethanol to the reaction flask, and add 160 mg NaBH.sub.4 (4.21 mmol, 1.2 eq) to the above system under an ice bath. Then, the reaction was stirred for 30 min in an ice bath, and then raised to room temperature and reacted for 3 h. After the most of the solution was removed under reduced pressure, water was added to the residue and extracted with EA, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated. The residue was purified with column chromatography (PE/EA=15:1) to give compound 14, 600 mg white solid, yield 75%.

(51) The structure is confirmed correct and data are as follow: MS (ESI) (m/z): 225.0 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 7.56 (d, J=2.6 Hz, 1H), 7.42 (dd, J=8.8, 2.6 Hz, 1H), 7.33 (dd, J=8.7, 1.6 Hz, 1H), 5.49 (t, J=5.8 Hz, 1H), 4.52 (d, J=5.9 Hz, 2H).

3. Synthesis of 4-((5-chloro-2-(trifluoromethoxy)benzyl)oxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-3)

(52) ##STR00026##

(53) With reference to the conditions of the fifth and six-step synthesis of Scheme I-1 in application case 1, the substituted benzyl alcohol was used to prepare substituted benzyl bromide, substituted benzyl bromide and 3-(Pyridin-3-ylamino)benzo[d]isoxazol-4-ol reaction yields the corresponding target compound.

(54) The structure is confirmed correct and data are as follow: Scheme I-3 MS(ESI) (m/z): 436.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.68 (s, 1H), 8.29 (s, 1H), 8.12 (d, J=4.5 Hz, 1H), 8.00 (d, J=8.3 Hz, 1H), 7.84 (s, 1H), 7.53-7.44 (m, 3H), 7.35-7.30 (m, 1H), 7.14 (d, J=8.4 Hz, 1H), 6.84 (d, J=8.0 Hz, 1H), 5.39 (s, 2H).

Application Case 4: Synthesis of 4-((2,6-dichlorobenzyl)oxy)-N-methyl-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-28)

(55) ##STR00027##

(56) Add 40 mg I-26 (0.10 mmol, 1.0 eq) and 5 mL DMF to the flask, add 5 mg sodium hydride (0.14 mmol, 1.3 eq) to the system under ice bath, then add 15 mg methyl iodide (0.104 mmol, 1.0 eq). After stirring the reaction for 15 min in an ice bath, and then raised to room temperature and reacted for 1 h, After the reaction was complete, water was added to the residue and extracted with EA, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated. The residue was purified with column chromatography (PE/EA=3:1) to give compound I-28, 20 mg white solid, yield 50%.

(57) The structure was confirmed correct and data are as follow: MS (ESI) (m/z): 401.0 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d6) δ 7.92 (d, J=2.7 Hz, 1H), 7.86-7.78 (m, 1H), 7.55 (t, J=8.2 Hz, 1H), 7.44 (d, J=3.3 Hz, 3H), 7.24 (d, J=8.5 Hz, 1H), 7.07 (ddd, J=8.2, 3.0, 1.4 Hz, 1H), 6.91 (d, J=8.0 Hz, 1H), 6.69 (dd, J=8.3, 4.7 Hz, 1H), 4.95 (s, 2H), 3.26 (s, 3H).

Application Case 5: Synthesis of 4-(benzyloxy)-N-(pyridin-3-yl)-1H-indazol-3-amine (Scheme I-29)

1. Synthesis of 4-(benzyloxy)-1H-indazol-3-amine (Compound 16)

(58) ##STR00028##

(59) Add 1.0 g 4 (4.4 mmol, 1.0 eq) and 4 mL 85% hydrazine hydrate, 10 mL ethanol to the reaction flask, and raise the temperature to 100° C. to react overnight. After the reaction is complete, evaporate the solvent, add 5 mL water and stir to obtain a suspension. The suspension was filtered to obtain compound 16, 840 mg white solid, yield 80%.

(60) The structure is confirmed correct and data are as follow: MS(ESI) (m/z): 240.0 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 11.39 (s, 1H), 7.47 (d, J=7.5 Hz, 2H), 7.37 (t, J=7.5 Hz, 2H), 7.29 (t, J=7.3 Hz, 1H), 7.04 (t, J=8.0 Hz, 1H), 6.73 (d, J=8.3 Hz, 1H), 6.35 (d, J=7.7 Hz, 1H), 5.17 (s, 2H), 4.90 (s, 2H).

2. Synthesis of 2-(4-(benzyloxy)-1H-indazol-3-yl)isoindoline-1,3-dione (Compound 17)

(61) ##STR00029##

(62) Add 240 mg 15 (1.0 mmol, 1.0 eq) and 148 mg phthalic anhydride (1.0 mmol, 1.0 eq) to the reaction flask, raise the temperature to 170° C. and react for 30 minutes. After the reaction is complete, cool to room temperature and add 5 mL EA, and then stirred to obtain a suspension, and filtered to obtain compound 17, 185 mg white solid, yield 50%.

(63) The structure is confirmed correct and data are as follow: MS(ESI) (m/z): 370.0 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 13.46 (s, 1H), 7.93-7.83 (m, 4H), 7.32 (t, J=8.0 Hz, 1H), 7.15 (d, J=8.4 Hz, 1H), 7.08-6.97 (m, 3H), 6.92 (t, J=7.6 Hz, 2H), 6.65 (d, J=7.7 Hz, 1H), 4.96 (s, 2H).

3. Synthesis of tert-butyl 4-(benzyloxy)-3-(1,3-dioxoisoindolin-2-yl)-1H-indazole-1-carboxylate (Compound 18)

(64) ##STR00030##

(65) Add 200 mg 17 (0.54 mmol, 1.0 eq) and 198 mg DMAP (1.63 mmol, 3.0 eq) to the reaction flask, then dissolve in 5 mL DCM, and then add 142 mg (Boc).sub.2O (0.65 mmol, 1.2 eq). After reacting at room temperature overnight, add water, extracted with DCM, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated. The residue was purified with column chromatography (PE/EA=6:1) to give compound 18, 80 mg white solid, yield 31%.

(66) The structure is confirmed correct and data are as follow: MS(ESI) (m/z): 470.0 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 7.85 (s, 4H), 7.71 (d, J=8.5 Hz, 1H), 7.61 (t, J=8.2 Hz, 1H), 7.04 (t, J=7.2 Hz, 1H), 7.02-6.96 (m, 3H), 6.94 (t, J=7.5 Hz, 2H), 4.99 (s, 2H), 1.63 (s, 9H).

4. Synthesis of tert-butyl 3-amino-4-(benzyloxy)-1H-indazole-1-carboxylate (Compound 19)

(67) ##STR00031##

(68) Add 50 mg 18 (0.11 mmol, 1.0 eq) and 6.9 mg 85% hydrazine hydrate (0.14 mmol, 1.4 eq), 3 mL ethanol to the reaction flask, and react at room temperature for 5 h. After the reaction is complete, evaporate the solvent, add water, extracted with DCM, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated. The residue was purified with column chromatography (PE/EA=4:1) to give compound 19, 20 mg white solid, yield 56%.

(69) The structure is confirmed correct and data are as follow: MS (ESI): m/z 340[M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 7.51-7.42 (m, 3H), 7.40-7.32 (m, 3H), 7.33-7.27 (m, 1H), 6.78 (d, J=7.9 Hz, 1H), 5.81 (s, 2H), 5.26 (s, 2H), 1.52 (s, 9H).

5. Synthesis of tert-butyl 4-(benzyloxy)-3-(pyridin-3-ylamino)-1H-indazole-1-carboxylate (Compound 20)

(70) ##STR00032##

(71) According to the conditions for synthesizing compound 8 in the third step in application case 1, the corresponding amino compound 19 is reacted with 3-bromopyridine (compound 7) to obtain the corresponding target compound 20.

(72) The structure is confirmed correct and data are as follow: MS (ESI): m/z 417[M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.74 (s, 1H), 8.18 (s, 1H), 8.15 (d, J=2.4 Hz, 1H), 8.14-8.10 (m, 1H), 7.55 (d, J=7.5 Hz, 2H), 7.52-7.43 (m, 2H), 7.40 (t, J=7.5 Hz, 2H), 7.37-7.29 (m, 2H), 6.89 (d, J=7.5 Hz, 1H), 5.40 (s, 2H), 1.59 (s, 9H).

6. Synthesis of 4-(benzyloxy)-N-(pyridin-3-yl)-1H-indazol-3-amine (Scheme I-29)

(73) ##STR00033##

(74) Add 50 mg 20 (0.12 mmol, 1.0 eq) to the reaction flask, dissolve in 5 mL dichloromethane, add 0.3 mL trifluoroacetic acid, react at room temperature for 2 h, after the reaction is complete, add water, extracted with DCM, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated. The residue was purified with column chromatography (PE/EA=3:1) to give compound I-29, 20 mg white solid, yield 53%.

(75) The structure is confirmed correct and data are as follow: MS (ESI): m/z 317[M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 12.16 (s, 1H), 8.54 (d, J=2.7 Hz, 1H), 7.98 (d, J=4.6 Hz, 1H), 7.96-7.91 (m, 1H), 7.86 (s, 1H), 7.48 (d, J=7.4 Hz, 2H), 7.36 (t, J=7.4 Hz, 2H), 7.30 (d, J=7.1 Hz, 1H), 7.23 (dd, J=8.4, 4.7 Hz, 1H), 7.16 (t, J=8.0 Hz, 1H), 6.89 (d, J=8.3 Hz, 1H), 6.49 (d, J=7.7 Hz, 1H), 5.27 (s, 2H).

Application Case 6: Synthesis of 4-(benzyloxy)-1-methyl-N-(pyridin-3-yl)-1H-indazol-3-amine (Scheme I-30)

1. Synthesis of 2-(4-(benzyloxy)-1-methyl-1H-indazol-3-yl)isoindoline-1,3-dione (Compound 21)

(76) ##STR00034##

(77) Add 150 mg 17 (0.41 mmol, 1.0 eq) and 112 mg K.sub.2CO.sub.3 (0.81 mmol, 2.0 eq) to the reaction flask, dissolve in 5 mL DMF, then add 75 mg methyl iodide (0.53 mmol, 1.3 eq) at room temperature. After overnight reaction, add water, extracted with DCM, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated. The residue was purified with column chromatography (PE/EA=4:1) to give compound 21, 100 mg white solid, yield 64%.

(78) The structure is confirmed correct and data are as follow: MS (ESI): m/z 384[M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 7.77 (t, J=1.8 Hz, 4H), 7.31-7.23 (m, 1H), 7.16 (dd, J=8.6, 1.8 Hz, 1H), 6.95-6.86 (m, 3H), 6.86-6.77 (m, 2H), 6.58 (dd, J=7.6, 1.7 Hz, 1H), 4.86 (s, 2H), 3.94 (s, 3H).

2. Synthesis of 4-(benzyloxy)-1-methyl-N-(pyridin-3-yl)-1H-indazol-3-amine (Scheme I-30)

(79) ##STR00035##

(80) According to the conditions for synthesizing compound 20 in the third and fourth steps in application case 5, the corresponding compound 21 is used in place of compound 18 to carry out the corresponding similar reaction to obtain the corresponding target compound I-30.

(81) The structure is confirmed correct and data are as follow: MS (ESI): m/z 311[M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.56 (d, J=2.7 Hz, 1H), 8.00 (d, J=4.7 Hz, 1H), 7.95 (dt, J=8.5, 2.0 Hz, 1H), 7.90 (s, 1H), 7.47 (d, J=7.4 Hz, 2H), 7.36 (t, J=7.4 Hz, 2H), 7.30 (d, J=7.2 Hz, 1H), 7.27-7.17 (m, 2H), 7.00 (d, J=8.4 Hz, 1H), 6.51 (d, J=7.7 Hz, 1H), 5.29 (s, 2H), 3.84 (s, 3H).

Application Case 7: Synthesis of 4-(benzyloxy)-N-(pyridin-3-yl)benzo[d]isothiazol-3-amine (Scheme I-31)

1. Synthesis of 4-(benzyloxy)benzo[d]isothiazol-3-amine (Compound 23)

(82) ##STR00036##

(83) Add 228 mg 4 (1.0 mmol, 1.0 eq) and 78 mg sodium sulfide (1.0 mmol, 1.0 eq) to the reaction flask, dissolve it in 5 mL DMSO under nitrogen, and react at 70° C. for 12 h. Then the whole reaction system was cooled to 0° C., 1.4 mL 25% aqueous ammonia solution and 1.4 mL of 15% sodium hypochlorite solution were added dropwise. The reaction was slowly warmed to room temperature and reacted for 5 h. After the reaction is complete, add water, extracted with EA, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated. The residue was purified with column chromatography (PE/EA=4:1) to give compound 23, 200 mg white solid, yield 78%.

(84) The structure is confirmed correct and data are as follow: MS (ESI): m/z 257 [M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 7.50 (s, 1H), 7.48 (s, 1H), 7.42-7.32 (m, 4H), 7.36-7.27 (m, 1H), 6.89 (d, J=7.4 Hz, 1H), 6.45 (s, 2H), 5.28 (s, 2H).

2. Synthesis of 4-(benzyloxy)-N-(pyridin-3-yl)benzo[d]isothiazol-3-amine (Scheme I-31

(85) ##STR00037##

(86) According to the conditions for synthesizing compound 8 in the third step in application case 1, the corresponding amino compound 23 is reacted with 3-bromopyridine (compound 7) to obtain the corresponding target compound I-31.

(87) The structure is confirmed correct and data are as follow: MS (ESI): m/z 334 [M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 9.03 (s, 1H), 8.44 (d, J=2.7 Hz, 1H), 8.23 (dd, J=8.6, 1.8 Hz, 1H), 8.12 (dd, J=4.7, 1.8 Hz, 1H), 7.62 (d, J=7.2 Hz, 2H), 7.58 (d, J=8.1 Hz, 1H), 7.53-7.38 (m, 4H), 7.30 (dd, J=8.6, 4.7 Hz, 1H), 7.07 (d, J=7.8 Hz, 1H), 5.42 (s, 2H).

Application Case 8: Synthesis of 8-(benzyloxy)-N-(pyridin-3-yl)isoquinolin-1-amine (Scheme I-32)

1. Synthesis of 8-(benzyloxy)isoquinoline (Compound 25)

(88) ##STR00038##

(89) Referring to the conditions for synthesizing compound 2 in the first step in application case 1, the corresponding hydroxy compound 24 is reacted with benzyl bromide to obtain the corresponding target compound 25.

(90) The structure is confirmed correct and data are as follow: MS (ESI): m/z 236[M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 10.70 (s, 1H), 7.48 (d, J=7.6 Hz, 2H), 7.39 (t, J=8.0 Hz, 1H), 7.24 (t, J=7.5 Hz, 2H), 7.14 (t, J=7.3 Hz, 1H), 6.99 (d, J=7.9 Hz, 1H), 6.94 (t, J=6.5 Hz, 1H), 6.87 (d, J=8.1 Hz, 1H), 6.25 (d, J=7.0 Hz, 1H), 5.07 (s, 2H).

2. Synthesis of 8-(benzyloxy)isoquinoline 2-oxide (compound 26)

(91) ##STR00039##

(92) Add 290 mg 26 (1.23 mmol, 1.0 eq) and 225 mg m-chloroperoxybenzoic acid (m-CPBA) (1.48 mmol, 1.2 eq) to the reaction flask, dissolve in 5 mL DCM, and react at room temperature for 12 h. Quench the reaction with saturated aqueous sodium carbonate, add water, extracted with EA, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated. The residue was purified with column chromatography (PE/EA=1:1) to give compound 26, 260 mg white solid, yield 84%.

(93) The structure is confirmed correct and data are as follow: MS (ESI): m/z 252[M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.61 (t, J=1.2 Hz, 1H), 8.02 (dd, J=7.1, 1.8 Hz, 1H), 7.76 (d, J=7.1 Hz, 1H), 7.43-7.34 (m, 4H), 7.28 (t, J=7.4 Hz, 2H), 7.21 (t, J=7.2 Hz, 1H), 7.12-7.06 (m, 1H), 5.18 (s, 2H).

3. Synthesis of 8-(benzyloxy)-1-chloroisoquinoline (Compound 27)

(94) ##STR00040##

(95) Add 200 mg 26 (0.80 mmol, 1.0 eq) and 1.5 mL phosphorus oxychloride (POCl.sub.3) to the reaction flask, and react at 90° C. for 5 h. After the reaction was completed, most of the solvent was distilled off under reduced pressure, water was added, the pH was adjusted to 8-9 with saturated sodium carbonate aqueous solution, extracted with DCM, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated to give compound 27. Without further purification, it was directly used in the next reaction.

4. Synthesis of 8-(benzyloxy)-N-(pyridin-3-yl)isoquinolin-1-amine (Scheme I-32)

(96) ##STR00041##

(97) Referring to the conditions for synthesizing compound 8 in the third step in application case 1, the corresponding compound 27 is reacted with 3-aminopyridine to obtain the corresponding target compound I-32.

(98) The structure is confirmed correct and data are as follow: MS (ESI): m/z 328[M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 10.01 (s, 1H), 8.19 (d, J=2.6 Hz, 1H), 8.14 (dd, J=8.6, 2.4 Hz, 1H), 8.08 (d, J=4.0 Hz, 1H), 7.97 (d, J=5.7 Hz, 1H), 7.72-7.64 (m, 2H), 7.63 (t, J=8.0 Hz, 1H), 7.54-7.45 (m, 3H), 7.40 (d, J=8.0 Hz, 1H), 7.27 (d, J=7.8 Hz, 1H), 7.23 (dd, J=8.3, 4.7 Hz, 1H), 7.13 (d, J=5.7 Hz, 1H), 5.40 (s, 2H).

Application Case 9: Synthesis of 4-((2-chloro-5-fluorobenzyl)oxy)-N-(pyrimidin-5-yl)benzo[d]isoxazol-3-amine (Scheme I-33)

1. Synthesis of 2-((2-chloro-5-fluorobenzyl)oxy)-6-fluorobenzonitrile (Compound 29)

(99) ##STR00042##

(100) Referring to the conditions of the first step in application case 1 to synthesize compound 4, the corresponding compound 2 and 28 are reacted to obtain the corresponding target compound 29.

(101) The structure is confirmed correct and data are as follow: MS (ESI): m/z 280 [M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 7.77-7.66 (m, 1H), 7.57 (dd, J=8.9, 5.1 Hz, 1H), 7.47 (dd, J=9.2, 3.1 Hz, 1H), 7.33-7.24 (m, 1H), 7.22 (d, J=8.6 Hz, 1H), 7.08 (t, J=8.8 Hz, 1H), 5.30 (s, 2H).

2. Synthesis of 4-((2-chloro-5-fluorobenzyl)oxy)benzo[d]isoxazol-3-amine (Compound 30)

(102) ##STR00043##

(103) Referring to the conditions for synthesizing compound 6 in the second step in application case 1, the corresponding compound 29 is reacted with 5 to obtain the corresponding target compound 30.

(104) The structure is confirmed correct and data are as follow: MS (ESI): m/z 293[M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 7.60-7.52 (m, 1H), 7.53-7.44 (m, 1H), 7.43-7.34 (m, 1H), 7.31-7.21 (m, 1H), 7.03-6.96 (m, 1H), 6.74 (d, J=8.0 Hz, 1H), 5.85 (s, 2H), 5.30 (s, 2H).

3. Synthesis of 4-((2-chloro-5-fluorobenzyl)oxy)-N-(pyrimidin-5-yl)benzo[d]isoxazol-3-amine (Scheme I-33)

(105) ##STR00044##

(106) Referring to the conditions for synthesizing compound 8 in the third step in application case 1, the corresponding compounds 30 and 31 are reacted to obtain the corresponding target compound I-33.

Application Case 10: Synthesis of N-(4-((2-chloro-5-fluorobenzyl)oxy)benzo[d]isoxazol-3-yl)-2-(dimethylamino) acetamide (Scheme I-34)

1. Synthesis of 2-chloro-N-(4-((2-chloro-5-fluorobenzyl)oxy)benzo[d]isoxazol-3-yl)acetamide (Compound 33)

(107) ##STR00045##

(108) Add 200 mg 30 (0.68 mmol, 1.0 eq) and 108 mg pyridine (1.36 mmol, 2.0 eq) to the reaction flask, dissolve in 10 mL DCM, then slowly add 116 mg chloroacetyl chloride (1.02 mmol, 1.5 eq). React at room temperature for 2 h. Then add water, extracted with DCM, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated. The residue was purified with column chromatography (PE/EA=2:1) to give compound 33, 130 mg white solid, yield 52%.

(109) The structure is confirmed correct and data are as follow: MS (ESI): m/z 370[M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 10.53 (s, 1H), 7.62-7.52 (m, 2H), 7.48 (dd, J=9.4, 3.0 Hz, 1H), 7.29 (d, J=8.5 Hz, 1H), 7.25 (dd, J=8.5, 3.0 Hz, 1H), 6.91 (d, J=8.0 Hz, 1H), 5.28 (s, 2H), 4.22 (s, 2H).

2. Synthesis of N-(4-((2-chloro-5-fluorobenzyl)oxy)benzo[d]isoxazol-3-yl)-2-(dimethylamino)acetamide (Scheme I-34)

(110) ##STR00046##

(111) Add 70 mg 33 (0.19 mmol, 1.0 eq) and 2 mL of 2M dimethylamine (4 mmol, 20.0 eq) in tetrahydrofuran to the reaction flask, dissolve in 5 mL acetonitrile, then add 66 mg potassium carbonate (0.48 mmol, 2.5 eq) and 15 mg potassium iodide, heated to 45° C. for 2 h. Add water, extracted with EA, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated. The residue was purified with column chromatography (PE/EA=1:1) to give compound I-34, 29 mg white solid, 40% yield.

(112) The structure is confirmed correct and data are as follow: MS (ESI): m/z 378[M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 10.10 (s, 1H), 7.67-7.57 (m, 3H), 7.40-7.31 (m, 1H), 7.28 (d, J=8.4 Hz, 1H), 7.03 (d, J=8.0 Hz, 1H), 5.31 (s, 2H), 3.00 (s, 2H), 1.96 (s, 6H).

Application Case 11: Synthesis of I-35˜I-38

(113) TABLE-US-00002 Scheme I R Yield (%) I-35 48 I-36 0 55 I-37 53 I-38 58

(114) According to the conditions for synthesizing the compound formula I-34 in the second step in application case 10, the corresponding alkylamine is reacted with the intermediate 31 to obtain the corresponding target compounds formula I-35 to I-38, that is: N-(4-((2-chloro-5-fluorobenzyl)oxy)benzo[d]isoxazol-3-yl)-2-(diethylamino) acetamide (scheme I-35); N-(4-((2-chloro-5-fluorobenzyl)oxy)benzo[d]isoxazol-3-yl)-2-(pyrrolidin-1-y) acetamide (scheme I-36); N-(4-((2-chloro-5-fluorobenzyl)oxy)benzo[d]isoxazol-3-yl)-2-(piperidin-1-yl)acetamide (scheme I-37); N-(4-((2-chloro-5-fluorobenzyl)oxy)benzo[d]isoxazol-3-yl)-2-morpholinoacetamide (scheme I-38).

(115) The structures were confirmed correct and data are as follow:

(116) Scheme I-35 MS(ESI) (m/z): 406.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 10.18 (s, 1H), 7.71-7.52 (m, 3H), 7.39-7.28 (m, 1H), 7.24 (d, J=8.4 Hz, 1H), 7.02 (d, J=8.1 Hz, 1H), 5.29 (s, 2H), 3.00 (s, 2H), 2.11 (q, J=7.2 Hz, 4H), 0.74 (t, J=7.2 Hz, 6H).

(117) Scheme I-36 MS(ESI) (m/z): 404.1 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 10.10 (s, 1H), 7.57-7.51 (m, 3H), 7.25 (q, J=8.6 Hz, 2H), 6.92 (d, J=8.0 Hz, 1H), 5.30 (s, 2H), 3.16 (s, 2H), 2.38 (s, 4H), 1.47 (s, 4H).

(118) Scheme I-37 MS(ESI) (m/z): 418 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 10.11 (s, 1H), 7.61-7.53 (m, 2H), 7.53-7.46 (m, 1H), 7.33-7.20 (m, 2H), 6.92 (d, J=8.1 Hz, 1H), 5.37 (s, 2H), 3.02 (s, 2H), 2.29 (s, 4H), 1.37-1.20 (m, 6H).

(119) Scheme I-38 MS(ESI) (m/z): 420 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 10.08 (s, 1H), 7.62-7.44 (m, 3H), 7.24 (d, J=8.5 Hz, 2H), 6.88 (d, J=7.8 Hz, 1H), 5.33 (s, 2H), 3.37 (s, 4H), 3.04 (s, 2H), 2.30 (s, 4H).

Application Case 12: Synthesis of 4-((2-phenylpyridin-4-yl)methoxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-39)

1. Synthesis of 2-phenylisonicotinaldehyde (compound 34)

(120) ##STR00053##

(121) Dissolve 500 mg 2-chloroisonicotinaldehyde (3.53 mmol, 1.0 eq) and 517 mg phenylboronic acid (4.24 mmol, 1.2 eq) in 50 mL toluene, add 204 mg tetrakis(triphenylphosphine)palladium (0.177 mmol), 0.05 eq) and 2N sodium carbonate (3.53 mL), heated to 90° C. for 12 h under nitrogen, add water, extracted with EA, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated. The residue was purified with column chromatography (PE/EA=15:1) to give compound 34, 455 mg white solid, yield 70%.

(122) The structure is confirmed correct and data are as follow: MS (ESI): m/z 184[M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 10.14 (s, 1H), 8.93 (dd, J=4.9, 0.9 Hz, 1H), 8.39 (s, 1H), 8.17-8.12 (m, 2H), 7.74 (dd, J=4.9, 1.4 Hz, 1H), 7.56-7.45 (m, 3H).

2. Synthesis of (2-phenylpyridin-4-yl)methanol (Compound 35)

(123) ##STR00054##

(124) Referring to the synthesis of compound 14.

(125) The structure is confirmed correct and data are as follow: MS (ESI): m/z 186[M+H].sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.57 (dd, J=5.0, 0.8 Hz, 1H), 8.08-8.01 (m, 2H), 7.85 (dd, J=1.6, 0.8 Hz, 1H), 7.52-7.44 (m, 2H), 7.43-7.38 (m, 1H), 7.30-7.26 (m, 1H), 5.49 (t, J=5.8 Hz, 1H), 4.60 (dt, J=5.8, 0.9 Hz, 2H).

3. Synthesis of 4-((2-phenylpyridin-4-yl)methoxy)-N-(pyridin-3-yl)benzo[d]isoxazol-3-amine (Scheme I-39)

(126) ##STR00055##

(127) According to the conditions of the fifth and six-step synthesis of the Scheme I-1 in application case 1, the substituted benzyl alcohol was used to prepare substituted benzyl bromide, followed by 3-(Pyridin-3-ylamino)benzo[d]isoxazol-4-ol reaction to obtain the corresponding target compound I-39.

(128) The structure is confirmed correct and data are as follow: Scheme I-39 MS(ESI) (m/z): 395 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.85 (d, J=2.8 Hz, 1H), 8.66 (d, J=5.0 Hz, 1H), 8.53 (s, 1H), 8.19 (dd, J=4.7, 1.4 Hz, 1H), 8.16 (s, 1H), 8.10 (dd, J=8.4, 2.8 Hz, 1H), 8.08-8.03 (m, 2H), 7.52 (d, J=8.2 Hz, 1H), 7.50-7.41 (m, 4H), 7.38-7.33 (m, 1H), 7.17 (d, J=8.4 Hz, 1H), 6.87 (d, J=8.0 Hz, 1H), 5.55 (s, 2H).

Application Case 13: Synthesis of Tert-Butyl 4-(3-((3-(pyridin-3-ylamino)benzo[d]isoxazol-4-yl)oxy)propyl)piperidine-1-carboxylate (Scheme I-40)

1. Synthesis of Tert-Butyl 4-(3-((methylsulfonyl)oxy)propyl)piperidine-1-carboxylate (Compound 38)

(129) ##STR00056##

(130) Dissolve 250 mg compound 37 (1.03 mmol, 1.0 eq) and 159 mg DIPEA (diisopropylethylamine) (1.23 mmol, 1.2 eq) in 5 mL dry DCM. Then 1.44 mg methanesulfonyl chloride (1.23 mmol, 1.2 eq) was added dropwise in the system under ice-water bath, and the reaction was carried out in an ice water bath for 15 min, then raised to room temperature and reacted overnight. Add water, extracted with DCM, the organic layers washed with saturated sodium chloride solution, dried over Na.sub.2SO.sub.4, filtered, and concentrated. It was used in the next reaction without further purification.

2. Synthesis of Tert-Butyl 4-(3-((3-(pyridin-3-ylamino)benzo[d]isoxazol-4-yl)oxy)propyl)piperidine-1-carboxylate (Scheme I-40)

(131) ##STR00057##

(132) Referring to the conditions for synthesizing scheme I-1 in the sixth step in application case 1, compound 38 was substituted for substituted benzyl bromide to react with 3-(Pyridin-3-ylamino)benzo[d]isoxazol-4-ol to obtain the corresponding target compound I-40.

(133) The structure is confirmed correct and data are as follow: Scheme I-40 MS(ESI) (m/z): 453 (M+H).sup.+. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 8.80 (t, J=3.3 Hz, 1H), 8.22 (ddt, J=6.2, 3.3, 1.5 Hz, 2H), 8.09 (ddt, J=8.3, 3.0, 1.7 Hz, 1H), 7.55 (t, J=8.1 Hz, 1H), 7.41 (dd, J=8.3, 4.7 Hz, 1H), 7.17 (dd, J=8.3, 6.4 Hz, 1H), 6.88 (t, J=7.1 Hz, 1H), 4.23 (t, J=6.4 Hz, 2H), 3.92 (s, 2H), 2.67 (s, 2H), 1.92 (d, J=7.6 Hz, 2H), 1.67 (d, J=12.9 Hz, 2H), 1.44 (d, J=29.6 Hz, 3H), 1.38 (s, 9H), 0.99 (dd, J=15.0, 10.4 Hz, 2H).

Application Case 14: Preparation of Hydrochloride Salt of Scheme I-2

(134) Dissolve 0.60 g compound I-2 (1.63 mmol, 1.0 eq) in 10 mL anhydrous ethyl acetate, and add 1.44 mL HCl (g) (1.8 mmol, 1.1 eq) in ethyl acetate (c=1.25 mol/L) to the above solution dropwise under ice-water bath, suction filtered after 10 minutes of reaction, and dried to obtain 0.53 g of white powdery solid in 80% yield.

Application Case 15: Determination of In Vitro Inhibition of Alkoxybenzeno Five- or Six-Member Heterocyclic Amines to Sphingomyelin Synthase 2

(135) Laboratory Instruments and Materials

(136) 1. Electric-heated thermostatic water bath (Shanghai Hengyi Science and Technology Co., Ltd.)

(137) 2. Vortex Mixers (XW-80A, Shanghai Jingke Industrial Co., Ltd.)

(138) 3. High-speed centrifuge (Eppendorf 5804R)

(139) 4. HPLC Agilent 1100 (Agilent Technologies, Palo Alto, Calif., USA), equipped with a quaternary pump, a vacuum degassing and an FLD fluorescence detector.

(140) 5. HPLC Column: COSMOSIL 5C18-MS-II (4.6 mm I.D.×250 mm).

(141) 6. DMPC. Purchased from Santa Cruz (USA) and dissolved in ethanol to prepare a solution of 40 mmol/L.

(142) 7. C6-NBD-Ceramide (6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl)-sphingosine). Purchased from Santa Cruz (USA) and dissolved in DMSO to prepare a solution of 1.16 mmol/L.

(143) 8. The organic solvents were purchased from Shanghai Sinopharm Reagent Company; methanol is of HPLC grade; water is ultrapure water filtrated by Milli-Q pump and deionized and ultrafiltrated by 0.22 μm ultrafiltration membrane. Other biological supplies are purchased in domestic companies.
9. Preparation of under tested compound solution: To each accurate weighed compound for 1˜2 mg, an appropriate amount of DMSO was added to formulate a stock solution of 3 mmol/L precisely. To a certain volume of the DMSO stock solution of the test compound, the appropriate volume of DMSO was added to dilute the solution to the desired concentration.
10. SMS1, SMS2 pure enzyme DDM solution and buffer were provided by Yu, Cao research group of National Protein Science Center (Shanghai).
Part 1. Activity Assay for Inhibition of Alkoxybenzeno Five- or Six-Member Heterocyclic Amines to Sphingomyelin Synthase 2

(144) Add 0.03 μL SMS2 pure enzyme DDM solution (total protein content 1.5 μg/μL), 1 μL test compound in DMSO solution or blank DMSO solution, 79.7 μL DDM buffer to 1.5 mL Eppendorf tube, vortex 30 seconds, let stand at room temperature for 5 min. Then add 20 μL DDM buffer containing 1 μL DMPC in ethanol (40 mmol/L) and 1 μL C6-NBD-Ceramide in DMSO (1.16 mmol/L). After vortexing for 30 seconds, Incubate for 0.5 h under water bath at 37° C. Then remove, add 200 μL of absolute ethanol, and vortex for 30 seconds. Take out 200 μL of the mixture and store it at 4° C. for high performance liquid chromatography analysis.

(145) Part 2. Activity Assay for Inhibition of Alkoxybenzeno Five- or Six-Member Heterocyclic Amines to Sphingomyelin Synthase 1

(146) Referring to the above SMS2 inhibitory activity detection method, and using SMS1 pure enzyme instead of SMS2 pure enzyme for corresponding operation.

(147) Using the same HPLC fluorogenic quantitative detecting method as reference (Xiaodong Deng; Hong Sun; et al. Analytical Letters, 2012, 45:12, 1581-1589) to analyses samples obtained above. Analysize and record peak areas of C6-NBD-SM (Asm) and C6-NBD-Ceramide (Acer) of each sample from blank group, positive control group (compound D2) and under tested compound group. Calculate inhibition rate from the formula below:

(148) $\mathrm{Inhibition}\mathrm{rate}\%=\frac{\mathrm{Blank}\left(\mathrm{Asm}\right)-\mathrm{Tested}\mathrm{compound}\left(\mathrm{Asm}\right)}{\mathrm{Blank}\left(\mathrm{Asm}\right)}\times 100$

(149) In vitro SMS2 inhibitory activity data of scheme I-1 I-38 obtained by HPLC fluorogenic quantitative detecting method are listed below:

(150) Part 3. Determination of SMS2 Median Inhibitory Concentration (IC50) of Alkoxybenzeno Five- or Six-Member Heterocyclic Amines (Scheme I-1˜I-38)

(151) The DMSO stock solution of under tested compound (6 mM) was diluted stepwise into five concentration gradient. 1 μL solution of each concentration was added into the trial system to prepare samples with the method mentioned in the first step of application case 15. The Asm values of the five concentration solution of tested compound were measured (compound D2 is a positive control), and the inhibition rate under the five concentration were calculated and fitted to obtain median inhibitory concentration (IC.sub.50). Each compound was measured three parallel groups. SMS2 median inhibitory concentration and SMS1 single inhibitory concentration (50 μM) of Scheme I-1˜I-38, and SMS1 median inhibitory concentration of some compounds are listed below in Table 1.

(152) Part 4. Determination of SMS1 Median Inhibitory Concentration (IC50) and SMS1 Single Concentration (50 μM) Inhibitory Concentration of Alkoxybenzeno Five- or Six-Member Heterocyclic Amines (Scheme I-1˜I-38)

(153) The experiment can be carried out with the corresponding concentration according to the operation similar to the determination of the median inhibitory concentration (IC50) of SMS2.

(154) TABLE-US-00003 TABLE 1 SMS2 Median Inhibitory Concentration and SMS1 Single Inhibitory Concentration (50 μM) of Scheme I-1~I-38 SMS2 SMS1 Inhibition SMS1 Selectivity Scheme IC.sub.50 (μM) (50 μM) IC.sub.50 (μM) ratio D2 20.9.sup.b  26% — — I-1  0.117  65% 40 342 I-2  0.102  44% 55 539 I-3  2.000  49% — — I-4  0.660   6% — — I-5  0.156   6% — — I-6  1.521   7% — — I-7  0.270  41% 79 292 I-8  0.094  44% 79 840 I-9  >10   5% — — I-10 0.498  41% 70 140 I-11 0.190  20% — — I-12 >10   5% — — I-13 0.255  21% — — I-14 0.486  25% — — I-15 >10   9% — — I-16 0.239  49% 48 201 I-17 0.523  17% — — I-18 >10  −5% — — I-19 0.107  59% 67 726 I-20 1.270  30% — — I-21 >10   5% — — I-22 4.139   4% — — I-23 0.945   8% — — I-24 >10 −18% — — I-25 0.153  53% 47 307 I-26 0.079  25% 70 886 I-27 0.720  30% — — I-28 10.000  29% — — I-29 10.000  −7% — — I-30 >10   1% — — I-31 1.459  13% — — I-32 >10  18% — — I-33 0.860  60% — — I-34 10.000   3% — — I-35 >10  11% — — I-36 >10   9% — — I-37 >10  60% — — I-38 >10  −1% — — I-39 13  −6% — — I-40 −8.3%   9% — — D2 56.2.sup.a — — — “a” refers to reference value. “b” refers to experimental value. “—” refers to not tested.