TRADITIONAL CHINESE MEDICINE COMPOSITION WITH IMPROVING COGNITION EFFECT, PREPARATION METHOD THEREOF AND TRADITIONAL CHINESE MEDICINE PREPARATION

Abstract

The present disclosure relates to the technical field of traditional Chinese medicine, and more specifically, to a traditional Chinese medicine composition with improving cognition effect, its preparation method and a traditional Chinese medicine preparation. In parts by weight, the traditional Chinese medicine composition is made from 1-20 parts of Gastrodiae Rhizoma, 1-15 parts of Polygala tenuifolia Willd., 1-30 parts of Acorus tatarinowii, 0.1-10 parts of Cistanche deserticola Ma, 0.1-10 parts of Rehmanniae Radix Praeparata, and 0.01-1 parts of curcumin. The pharmacodynamic substances of Gastrodiae Rhizoma, Polygala tenuifolia Willd., Acorus tatarinowii, Cistanche deserticola Ma and Rehmanniae Radix Praeparata are extracted completely, concentrated and dried as much as possible. The volatile oil of Acorus tatarinowii and curcumin are encapsulated and dried by the volatile oil encapsulation technology. The dry powder of the traditional Chinese medicine compound extract is evenly mixed with the volatile oil encapsulation compound.

Claims

1. A traditional Chinese medicine composition with improving cognition effect, wherein the traditional Chinese medicine composition is made from following raw materials: 16-20 parts by weight of Gastrodiae Rhizoma, 10-14 parts by weight of Polygala tenuifolia Willd., 16-20 parts by weight of Acorus tatarinowii, 5-7 parts by weight of Cistanche deserticola Ma, 5-7 parts by weight of Rehmanniae Radix Praeparata, and 0.05-0.15 parts by weight of curcumin; a preparation method for the traditional Chinese medicine composition comprises the following steps: ethanol extraction: heating and refluxing Gastrodiae Rhizoma and Polygala tenuifolia Willd. with ethanol aqueous solution, and collecting an ethanol extraction solution and an ethanol extraction residue; water extraction: heating and refluxing Acorus tatarinowii with water, and collecting Acorus tatarinowii solution, Acorus tatarinowii residue and Acorus tatarinowii volatile oil; heating and refluxing Cistanche deserticola Ma, Rehmanniae Radix Praeparata, the ethanol extraction residue, the Acorus tatarinowii residue with water, and collecting a water extraction solution; filtration and concentration: mixing the ethanol extraction solution, the Acorus tatarinowii solution and the water extraction solution to obtain a mixing solution, and filtrating and concentrating the mixing solution to obtain an extractum; encapsulation: mixing the Acorus tatarinowii volatile oil and curcumin, and obtaining micropellets by cyclodextrin encapsulating; and mixing: mixing the extractum and the micropellets.

2. The traditional Chinese medicine composition of claim 1, wherein the traditional Chinese medicine composition is made from following raw materials: 18 parts by weight of Gastrodiae Rhizoma, 12 parts by weight of Polygala tenuifolia Willd., 18 parts by weight of Acorus tatarinowii, 6 parts by weight of Cistanche deserticola Ma, 6 parts by weight of Rehmanniae Radix Praeparata, and 0.1 parts by weight of curcumin.

3. The preparation method for the traditional Chinese medicine composition of claim 1, comprising the following steps: ethanol extraction: heating and refluxing Gastrodiae Rhizoma and Polygala tenuifolia Willd. with ethanol aqueous solution, and collecting an ethanol extraction solution and an ethanol extraction residue; water extraction: heating and refluxing Acorus tatarinowii with water, and collecting Acorus tatarinowii solution, Acorus tatarinowii residue and Acorus tatarinowii volatile oil; heating and refluxing Cistanche deserticola Ma, Rehmanniae Radix Praeparata, the ethanol extraction residue, the Acorus tatarinowii residue with water, and collecting a water extraction solution; filtration and concentration: mixing the ethanol extraction solution, the Acorus tatarinowii solution and the water extraction solution to obtain a mixing solution, and filtrating and concentrating the mixing solution to obtain an extractum; encapsulation: mixing the Acorus tatarinowii volatile oil and curcumin, and obtaining micropellets by cyclodextrin encapsulating; and mixing: mixing the extractum and the micropellets.

4. The preparation method of claim 3, wherein in the ethanol extraction step, a volume percentage concentration of the ethanol aqueous solution is 50-80%.

5. The preparation method of claim 3, wherein in the ethanol extraction step, the ethanol aqueous solution is used in an amount of 8-20 times of the total weight of the Gastrodiae Rhizoma and the Polygala tenuifolia Willd.; a number of heating and refluxing is 1-5 times, and 0.5-3 hours each time.

6. The preparation method of claim 3, wherein in the water extraction step, 8-30 times the weight of the water is added into the Acorus tatarinowii for heating and refluxing, and a number of heating and refluxing is 1-3 times, and 3-5 hours each time, and the Acorus tatarinowii solution, the Acorus tatarinowii residue and the Acorus tatarinowii volatile oil are collected; water 8-30 times the total weight of medicinal materials is added into the Acorus tatarinowii solution, Acorus tatarinowii residue and Acorus tatarinowii volatile oil, a the number of heating and refluxing is 1-5 times, and 0.5-3 hours each time, and the water extraction solution is collected.

7. The preparation method of claim 3, wherein in the encapsulation step, a weight ratio of the mixture of the Acorus tatarinowii volatile oil and the curcumin to β-cyclodextrin is 1:(1-5); a polishing time during the encapsulation is 1-4 hours, and a drying temperature is 40-60° C.

8. A traditional Chinese medicine preparation, wherein the traditional Chinese medicine preparation is composed of the traditional Chinese medicine composition of claim 1 and pharmaceutically acceptable excipients.

9. The traditional Chinese medicine preparation of claim 8, wherein a dosage form of the traditional Chinese medicine preparation is a capsule, a tablet or a granule.

Description

DETAILED DESCRIPTION OF THE EMBODIMENTS

[0039] A traditional Chinese medicine composition with improving cognition effect, a preparation method of the traditional Chinese medicine composition and a traditional Chinese medicine preparation are disclosed. Those skilled in the art can learn from the contents of the description and appropriately improve the realization of process parameters. In particular, it should be noted that all similar substitutions and modifications are obvious to those skilled in the art, and they are considered to be included in the present disclosure. The method and application of the disclosure have been described through preferred embodiments. It is obvious that relevant personnel can realize and apply the technology of the disclosure by changing or appropriately changing and combining the method and application described herein without departing from the content, spirit and scope of the disclosure.

[0040] The raw materials or excipients used in the traditional Chinese medicine composition with improving cognition effect, its preparation method and the traditional Chinese medicine preparation can be purchased from the market.

[0041] The disclosure is further described below in combination with the embodiments.

[0042] Embodiment 1 Traditional Chinese medicine composition and its preparation method

[0043] 1. The formula of this embodiment was:

[0044] Gastrodiae Rhizoma 180 g, Polygala tenuifolia Willd. 120 g, Acorus tatarinowii 180 g, Cistanche deserticola Ma 60 g, Rehmanniae Radix Praeparata 60 g, and curcumin 1 g.

[0045] 2. The preparation method was as follows.

[0046] (1) Gastrodiae Rhizoma and Polygala tenuifolia Willd. were heated and refluxed with 10 times of 70% ethanol aqueous solution for 3 times, 1 hour each time, and the solution and residue were collected respectively.

[0047] (2) Acorus tatarinowii was heated and refluxed with 14 times of water once for 5 hours, and the solution, residue and volatile oil were collected.

[0048] (3) Cistanche deserticola Ma, Rehmanniae Radix Praeparata, the residue collected in steps (1) and (2) are heated and refluxed with 10 times of water twice for 1.5 hours each time, and the solution was collected.

[0049] (4) All the solution obtained in steps (1), (2) and (3) was filtrated and concentrated to obtain the extractum.

[0050] (5) The volatile oil obtained in step (2) and curcumin were mixed, and the micropellets were obtained by cyclodextrin encapsulating. The weight ratio of the mixture of the volatile oil and curcumin to β-cyclodextrin was 1:1.5, the grinding time was 2 hours, and the drying temperature was 45° C.

[0051] (6) The extractum obtained in step (4) and the micropellets obtained in step (5) were mixed to obtain the traditional Chinese medicine composition.

Comparative Example 1

[0052] 1. The formula of this example was:

[0053] Gastrodiae Rhizoma 180 g, Polygala tenuifolia Willd. 120 g, Acorus tatarinowii 180 g, Cistanche deserticola Ma 60 g, Rehmanniae Radix Praeparata 60 g, and curcumin 1 g.

[0054] 2. The preparation method was as follows.

[0055] (1) Gastrodiae Rhizoma was grinded into powder and passed through No. 6 pharmacopoeia sieve.

[0056] (2) Polygala tenuifolia Willd., Acorus tatarinowii, Cistanche deserticola Ma and Rehmanniae Radix Praeparata were decocted with 10 times the amount of water twice for 2 hours each time, and the solution was collected.

[0057] (3) All the solution obtained in step (2) was filtrated and concentrated to obtain the extractum.

[0058] (4) The extractum obtained in step (2), the Gastrodiae Rhizoma powder obtained in step (1) and the curcumin were mixed.

Comparative Example 2

[0059] 1. The formula of this example was:

[0060] Gastrodiae Rhizoma 180 g, Polygala tenuifolia Willd. 120 g, Acorus tatarinowii 180 g, Cistanche deserticola Ma 60 g, Rehmanniae Radix Praeparata 60 g, and curcumin 1 g.

[0061] 2. The preparation method was as follows.

[0062] (1) The crude Gastrodiae Rhizoma powder was heated and refluxed with 70% ethanol aqueous solution for 3 times, 2 hours each time. The drug-liquid ratio was 10 times, 8 times and 8 times respectively. The extracted solution was collected, filtrated and cyclone dried to obtain a dry extract.

[0063] (2) The Polygala tenuifolia Willd. was heated and refluxed with 80% ethanol aqueous solution for 3 times, 2 hours each time. The drug-liquid ratio was 10 times, 8 times and 8 times respectively. The extracted solution was collected, filtrated and cyclone dried to obtain a dry extract.

[0064] (3) The Acorus tatarinowii was heated and refluxed with 10 times the amount of water for 3 times, 2 hours each time. The drug-liquid ratio was 10 times, 8 times and 8 times respectively. The extracted solution was collected, filtrated and cyclone dried to obtain a dry extract.

[0065] (4) The Cistanche deserticola Ma was heated and refluxed with 10 times the amount of water for 3 times, 2 hours each time. The drug-liquid ratio was 10 times, 8 times and 8 times respectively. The extracted solution was collected, filtrated and cyclone dried to obtain a dry extract.

[0066] (5) The Cistanche deserticola Ma was heated and refluxed with 10 times the amount of water for 3 times, 2 hours each time. The drug-liquid ratio was 10 times, 8 times and 8 times respectively. The extracted solution was collected, filtrated and cyclone dried to obtain a dry extract.

[0067] (6) The Rehmanniae Radix Praeparata was heated and refluxed with 10 times the amount of water for 3 times, 2 hours each time. The drug-liquid ratio was 10 times, 8 times and 8 times respectively. The extracted solution was collected, filtrated and cyclone dried to obtain a dry extract.

[0068] (7) The dry extracts obtained in steps (1)-(6) were mixed with the curcumin.

Comparative Example 3

[0069] 1. The formula of this example was:

[0070] Gastrodiae Rhizoma 300 g, Pheretima 200 g, Acorus tatarinowii 300 g, Polygala tenuifolia Willd. 200 g, Rehmanniae Radix Praeparata 100 g, and Cistanche deserticola Ma 100 g.

[0071] 2. The preparation method was as follows.

[0072] (1) Gastrodiae Rhizoma, Pheretima, Acorus tatarinowii, Polygala tenuifolia Willd., Rehmanniae Radix Praeparata, and Cistanche deserticola Ma were decocted with 10 times the amount of water twice for 2 hours each time, and the solution was collected.

[0073] (2) All the solution obtained in step (1) was filtrated and concentrated to obtain the extractum.

Embodiment 1 Efficacy Test

[0074] Drugs to be tested: Embodiment 1 group, comparative example 1 group, comparative example 2 group and comparative example 3 group. Positive drug: huperzine-A.

[0075] 1. Object Recognition Test

[0076] Test Method:

[0077] Adaptation period: on the first day, the mice were placed in the test room for 1 hour of environmental adaptation and familiarity, and then placed in the open field box for 20 minutes (there was no object in the box), so as to eliminate the impact of the open field box on the tested mice. After the experiment of each mouse, the open field box was cleaned with 75% ethanol.

[0078] Familiarization period: on the second day (24 hours after the adaptation period), the mice were placed in the test room for 1 hour of environmental adaptation and familiarization, then two yellow cylindrical bottles of the same size and texture were placed at the left and right ends of the same side of the open field box as familiar objects, and then the mouse was put into the box with its back towards the objects. The mouse's exploration of the objects within 5 min was recorded (timing standard: the exploration time within 2 cm of the mouse's nose or mouth from the object, and its lying on the objects or just walking around the objects was not included). After the experiment of each mouse, the open field box was cleaned with 75% ethanol.

[0079] Test period: 2 h after the familiarization period, replace a yellow cylindrical bottle in the open field box was replaced with a green conical bottle of the same size as a strange object, and then the mouse was put into the box with its back towards the objects. The total exploration time of novel object (Tn) and the total exploration time of familiar object (Tf) were recorded. The recognition index (RI) was calculated: RI=Tn/(Tn+Tf). After the experiment of each mouse, the open field box was cleaned with 75% ethanol.

[0080] Statistical methods: the data were analyzed by GraphPad Prism 7.01 software (GraphPad Software, Inc., San Diego, Calif., USA). Before the parameter test of all group tests, the data were tested by normality test (Kolmogorov-Smirnov test) and variance homogeneity test (Levene test). Except for the escape latency in water maze, all test parameters were compared between groups by non paired T-test (single tail), and the test level was set as p<0.05. The escape latency in water maze was compared between two groups by two-way analysis of variance. The post test was conducted by Sidak's multiple comparisons test, and the test level was set as p<0.05. The results are shown in Table 1:

TABLE-US-00001 TABLE 1 Effects of the drugs to be tested on the recognition index in the novel object recognition test of mice Dose Number of Recognition index Groups (crude drug) animals/pieces (x□SE) Control group — 17 0.5715 ± 0.06626  Model group — 17 0.4180 ± 0.02726* Positive drug 0.67 tablets/kg 17 0.5341 ± 0.04280# group Embodiment 1 1.002 g/kg 17 0.5322 ± 0.04504# group Comparative 1.002 g/kg 17 0.5081 ± 0.05318  example 1 group Comparative 1.002 g/kg 17 0.5269 ± 0.04310# example 2 group Comparative 1.200 g/kg 17 0.5050 ± 0.03855# example 3 group Note: compared with the control group, *p < 0.05; compared with the model group, #p < 0.05.

[0081] Novel object recognition test is a fine and sensitive behavioral method to detect the recognition memory of animals by using the instinct of animals to approach and explore novel objects. It can be seen from the statistical data that the recognition index of the model group decreased significantly compared with the normal control group, while the recognition index of the positive drug group, embodiment 1 group, comparative example 1 group, comparative example 3 group increased significantly after intragastric administration. The results showed that the declarative memory ability of mice in the model group decreased and the model was successfully established. The declarative memory ability of stressed mice could be improved after the treatment of positive drug group, embodiment 1 group, comparative example 1 group, comparative example 3 group. In the embodiment 1 group, the recognition index was the highest and closest to the control group.

[0082] 2. Spatial Learning Ability Test of Mice by Step-Down Test

[0083] Test method: the grouping, sample preparation and modeling of mouse step-down test were the same as above. At the beginning of the test, the step-down training was carried out, where the adaptation was carried out for 3 min and then the power on training was carried out for 300 s. The test was conducted after 24 hours. The drug was administered 1 hour before the test, and scopolamine hydrobromide and normal saline were injected intraperitoneally 15-20 minutes before the test. Then the mouse was put on the platform and the latency of jumping off the platform for the first time and the number of errors (getting off the platform) within 300 s were recorded. If the mouse did not jump off the platform within 300 s, the number of errors was recorded as 0 and the latency was recorded as 300 s.

[0084] The results are shown in Table 2 and 3:

TABLE-US-00002 TABLE 2 Effects of the drugs to be tested on the latency in the step-down test of mice Dose Number of Groups (crude drug) animals/pieces Latency/s Control group — 17 100.3 ± 31.82  Model group — 17 42.01 ± 13.95* Positive drug 0.67 tablets/kg 17 94.12 ± 29.48# group Embodiment 1 1.002 g/kg 17  137.7 ± 25.71## group Comparative 1.002 g/kg 17 103.5 ± 28.91# example 1 group Comparative 1.002 g/kg 17 108.2 ± 25.66# example 2 group Comparative 1.200 g/kg 17 83.39 ± 23 .71  example 3 group Note: compared with the control group, *p < 0.05, **p < 0.01; compared with the model group, #p < 0.05, ##p < 0.01.

TABLE-US-00003 TABLE 3 Effects of the drugs to be tested on the number of errors in the step-down test of mice Dose Number of Number of errors Groups (crude drug) animals/pieces (x□SE) Control group — 17 1.375 ± 0.2869 Model group — 17  2.714 ± 0.3696** Positive drug 0.67 tablets/kg 17  1.833 ± 0.3445# group Embodiment 1 1.002 g/kg 17  1.667 ± 0.3333# group Comparative 1.002 g/kg 17 2.471 ± 0.5153 example 1 group Comparative 1.002 g/kg 17 2.600 ± 0.5589 example 2 group Comparative 1.200 g/kg 17 2.824 ± 0.5767 example 3 group Note: compared with the control group, *p < 0.05, **p < 0.01; compared with the model group, #p < 0.05, ##p < 0.01.

[0085] After intragastric administration, the latency of the model group was significantly shorter than that of the control group, the number of errors was significantly increased, and the model was successfully established. After intragastric administration, the latencies of the positive drug group, embodiment 1 group, comparative example 1 group and comparative example 2 group were significantly longer than that of the model group. The numbers of errors in positive drug group and embodiment 1 group were significantly lower than that in the model group. The results showed that the memory ability of the model group mice in the step-down test was defective. The positive drug group and embodiment 1 group could improve the memory function defect of the step-down test. The above research shows that the drug combination of the disclosure can improve the defect of spatial memory ability of mice to a certain extent and can significantly improve the memory ability of step-down test of mice.

[0086] 3. Spatial Learning Ability Test of Mice by Morris Water Maze Test

[0087] Implementation method: the test procedure included two parts: directional navigation and space exploration. A total of 5 consecutive days were tested. The first part was the directional navigation experiment, which was tested on mice for 4 days, and the second part was the space exploration experiment, which was tested for 1 day. The melanin edible toner was washed away with warm water to hide the platform, and it was kept in sharp contrast with the color of mice during camera shooting for easy tracking. The platform was located 0.8 cm below the water surface and the water depth was 21 cm. It was located in the NW quadrant. The water temperature in the water maze was maintained at a constant temperature of 22-24° C. with a heating rod.

[0088] Directional navigation experiment: each mouse was trained 4 times a day, with an interval of 15-20 min. During the test, the water entry point was selected in a semi random way each time. The experimenter held the mouse by hand, made it face the pool wall, and gently put it into the water. In each training, if the mouse could find the platform within 60 s, and the time spent on the platform was more than 2 S, it was regarded as the sign of successful platform search. The time spent by the mice from entering the water to successful platform search was recorded as the latency. If the mouse failed to find the platform within 60 s, the experimenter guided it to the platform by hand, and the latency was 60 s. After the mice boarded the platform, the mice were kept on the platform for 10 s, so that they could carry out spatial learning and memory according to the reference objects of the four quadrants, and the tension of the mice were reduced. The average value of daily latency was calculated to evaluate the acquisition ability of animals' spatial memory.

[0089] Space exploration experiment: the platform was removed and each mouse was tested once for 60 s. The SE quadrant was selected and the mouse was gently put into the water facing the edge of the pool wall. The spatial memory ability of the animal was evaluated by the number of times the animal crossed the original platform position within 60 s, the run ratio and time ratio of the original platform quadrant (i.e. the ratio of the run and time of the animal's original platform quadrant to the total run and time).

[0090] The results are shown in table 4-8:

TABLE-US-00004 TABLE 4 Effects of the drugs to be tested on escape latency in water maze test of mice Dose Number of First day (s) Second day (s) Third day (s) Fourth day (s) Groups (crude drug) animals/pieces (x□SE) (x□SE) (x□SE) (x□SE) Control group — 17 45.8 ± 2.51 34.0 ± 2.90 26.1 ± 2.50 27.0 ± 2.68 Model group — 17 53.2 ± 2.18 44.2 ± 2.77 42.4 ± 2.93   47.7 ± 2.37**** Positive drug group 0.67 tablets/kg 17 52.7 ± 2.29 44.5 ± 2.80 43.6 ± 2.91 43.5 ± 2.83 Embodiment 1 group 1.002 g/kg 17 50.3 ± 2.48 40.5 ± 2.92 39.0 ± 3.12 35.8 ± 2.76 Comparative 1.002 g/kg 17 49.9 ± 2.56 49.9 ± 2.33 46.2 ± 2.71 39.0 ± 2.75 example 1 group Comparative 1.002 g/kg 17 46.4 ± 2.89 46.8 ± 2.74 46.3 ± 2.84 40.6 ± 2.81 example 2 group Comparative 1.200 g/kg 17 49.9 ± 2.50 44.1 ± 2.69 44.9 ± 2.80 45.5 ± 2.54 example 3 group

TABLE-US-00005 TABLE 5 Effects of the drugs to be tested on the escape latency in the exploration period in the water maze test of the mice Dose Number of Escape latency/s Groups (crude drug) animals/pieces (x□SE) Control group — 17 20.05 ± 3.983  Model group — 17  39.27 ± 4.814** Positive drug 0.67 tablets/kg 17 22.91 ± 3.922# group Embodiment 1 1.002 g/kg 17 24.45 ± 4.652# group Comparative 1.002 g/kg 17 32.12 ± 5.158  example 1 group Comparative 1.002 g/kg 17 30.16 ± 5.256  example 2 group Comparative 1.200 g/kg 17 27.10 ± 4.234# example 3 group

TABLE-US-00006 TABLE 6 Effects of the drugs to be tested on the stay time in the target quadrant during the exploration escape latency in the water maze test of the mice Stay time in target Dose Number of quadrant/s Groups (crude drug) animals/pieces (x□SE) Control group — 17 17.30 ± 0.8384 Model group — 17 14.15 ± 1.354* Positive drug 0.67 tablets/kg 17 15.04 ± 0.9555 group Embodiment 1 1.002 g/kg 17 16.04 ± 0.7052 group Comparative 1.002 g/kg 17 15.60 ± 0.9211 example 1 group Comparative 1.002 g/kg 17 15.11 ± 0.8451 example 2 group Comparative 1.200 g/kg 17 15.88 ± 1.3682 example 3 group

TABLE-US-00007 TABLE 7 Effects of the drugs to be tested on the target quadrant run distance in the exploration period in the water maze test of the mice Run distance of Dose Number of target quadrant/mm Groups (crude drug) animals/pieces (x□SE) Control group — 17 2821 ± 167.1 Model group — 17 2373 ± 175.4 Positive drug 0.67 tablets/kg 17 2867 ± 207.6 group Embodiment 1 1.002 g/kg 17 2804 ± 143.7 group Comparative 1.002 g/kg 17 2531 ± 208.0 example 1 group Comparative 1.002 g/kg 17 2413 ± 178.2 example 2 group Comparative 1.200 g/kg 17 2539 ± 175.9 example 3 group

TABLE-US-00008 TABLE 8 Effects of the drugs to be tested on the number of crossing the platform in the exploration period in the water maze test of the mice Number of Dose Number of crossing platform Groups (crude drug) animals/pieces (x□SE) Control group — 17 3.125 ± 0.3966 Model group — 17  1.308 ± 0.3820** Positive drug 0.67 tablets/kg 17  2.786 ± 0.4591# group Embodiment 1 1.002 g/kg 17  2.647 ± 0.4922# group Comparative 1.002 g/kg 17 2.006 ± 0.4180 example 1 group Comparative 1.002 g/kg 17 1.967 ± 0.4008 example 2 group Comparative 1.200 g/kg 17 2.433 ± 0.4563 example 3 group

[0091] Note (Table 48): compared with the control group, *p<0.05,**p<0.01,****p <0.0001; compared with the model group, #p<005.

[0092] After intragastric administration, the number of crossing the platform in the model group was significantly lower than that in the control group, and increased after the intervention of the drug to be test. The run ratio and time of the target quadrant were significantly higher than those in the model group. It is suggested that the drug to be tested can improve the defect of spatial memory ability in mice to a certain extent. On the 4th day of the water maze test, the escape latency of the model group was significantly longer than that of the control group, suggesting that the memory storage ability of the model group mice was defective. During the exploration period of water maze, the escape latency in the model group was significantly longer than that in the control group, and the stay time in the target quadrant and the number of crossing the platform in the model group were significantly lower than those in the control group, suggesting that the memory retrieval ability of the model group was impaired. After intragastric administration, the escape latency of the positive drug group, embodiment 1 group and comparative example 3 group was significantly shorter than that of the model group, and the number of crossing the platform was significantly higher than that of the model group, suggesting that the positive drug, embodiment 1 group and comparative example 3 group could improve the defect of spatial memory retrieval ability of mice, and the defect of spatial extraction ability of mice could be improved in different degrees in the comparative example 1 group and comparative example 2 group.

[0093] Based on the results of classic animal memory triple behavioral tests, namely object recognition test, step-down test and water maze test, the results show that the formula of the disclosure has the trend and effect of improving cognition and memory of mice, and the optimized preparation process can better realize the drug effect, which is better than the traditional extraction process.

[0094] The above is only the preferred embodiments of the disclosure. It should be pointed out that for ordinary technicians in the technical field, several improvements and refinements can be made without departing from the principles of the disclosure, and these improvements and refinements should also be regarded as the protection scope of the disclosure.