SUBSTITUTED 1,2,4-OXADIAZOLE, ITS APPLICATION AND A PHARMACEUTICAL PREPARATION COMPRISING IT

Abstract

Disclosed are compounds effective against tuberculosis based on substituted 1,2,4-oxadiazoles of general formula I, where Y═S or CH.sub.2 and R=phenyl- or phenyl-substituted in positions 2, 3, 4, and 5 by one or several electron-acceptor groups or electron-donor groups. These compounds can be produced by easy syntheses and are characterized by low toxicity and high efficacy against mycobacteria, including multiresistant strains thereof. Also disclosed are a pharmaceutical preparation containing substituted 1,2,4-oxadiazole of formula I as an active substance as well as the use of this substituted 1,2,4-oxadiazole as an antituberculosis drug.

Claims

1. A substituted 1,2,4-oxadiazole with general formula I ##STR00028## where Y═S, CH.sub.2; R is selected from the group comprising: phenyl- or phenyl-substituted in positions 2, 3, 4, and 5 by one or several electron-acceptor groups including —NO.sub.2, —N+(C.sub.1-C.sub.4 alkyl).sub.3, —CF.sub.3, CCl.sub.3, —CN, —COOH, —COO(C.sub.1-C.sub.4 alkyl), —COOAryl, —CHO, —CO(C.sub.1-C.sub.4 alkyl), —COAryl, —F, —Cl, —Br, or —I, and/or by one or several electron-donor groups including —NH.sub.2, —NH(C.sub.1-C.sub.4 alkyl), —N(C.sub.1-C.sub.4 alkyl).sub.2, —OH, —O—(C.sub.1-C.sub.4 alkyl), —Oaryl, —NHCOCH.sub.3, —NHCO—(C.sub.1-C.sub.4 alkyl); —NHCOaryl, or —(C.sub.1-C.sub.4 alkyl).

2. The substituted 1,2,4-oxadiazole of general formula I according to claim 1, where Y is S.

3. The substituted 1,2,4-oxadiazole of general formula I according to claim 1, where Y is CH.sub.2.

4. (canceled)

5. (canceled)

6. A pharmaceutical composition useful for treating tuberculosis, comprising a substituted 1,2,4-oxadiazole with general formula I ##STR00029## where Y═S, CH.sub.2; R is selected from the group comprising: phenyl- or phenyl-substituted in positions 2, 3, 4, and 5 by one or several electron-acceptor groups including —NO.sub.2, —N+(C.sub.1-C.sub.4 alkyl).sub.3, —CF.sub.3, CCl.sub.3, —CN, —COOH, —COO(C.sub.1-C.sub.4 alkyl), —COOAryl, —CHO, —CO(C.sub.1-C.sub.4 alkyl), —COAryl, —F, —Cl, —Br, or —I, and/or by one or several electron-donor groups including —NH.sub.2, —NH(C.sub.1-C.sub.4 alkyl), —N(C.sub.1-C.sub.4 alkyl).sub.2, —OH, —O—(C.sub.1-C.sub.4 alkyl), —Oaryl, —NHCOCH.sub.3, —NHCO—(C.sub.1-C.sub.4 alkyl); —NHCOaryl, or —(C.sub.1-C.sub.4 alkyl), in a pharmaceutically acceptable carrier therefor.

7. The composition of claim 6, wherein in the substituted 1,2,4-oxadiazole of general formula I, Y is S.

8. The composition of claim 6, wherein in the substituted 1,2,4-oxadiazole of general formula I, Y is CH.sub.2.

9. The composition of claim 6, wherein the pharmaceutically acceptable carrier comprises a dry carrier.

10. The composition of claim 6, wherein the pharmaceutically acceptable carrier comprises a wet carrier.

11. A method for treatment of tuberculosis in a patient in need of said treatment, comprising administering to said patient, a therapeutically effective amount of a substituted 1,2,4-oxadiazole with general formula I ##STR00030## where Y═S, CH.sub.2; R is selected from the group comprising: phenyl- or phenyl-substituted in positions 2, 3, 4, and 5 by one or several electron-acceptor groups including —NO.sub.2, —N+(C.sub.1-C.sub.4 alkyl).sub.3, —CF.sub.3, CCl.sub.3, —CN, —COOH, —COO(C.sub.1-C.sub.4 alkyl), —COOAryl, —CHO, —CO(C.sub.1-C.sub.4 alkyl), —COAryl, —F, —Cl, —Br, or —I, and/or by one or several electron-donor groups including —NH.sub.2, —NH(C.sub.1-C.sub.4 alkyl), —N(C.sub.1-C.sub.4 alkyl).sub.2, —OH, —O—(C.sub.1-C.sub.4 alkyl), —Oaryl, —NHCOCH.sub.3, —NHCO—(C.sub.1-C.sub.4 alkyl); —NHCOaryl, or —(C.sub.1-C.sub.4 alkyl).

12. The method of claim 11, wherein in the substituted 1,2,4-oxadiazole of general formula I, Y is S.

13. The method of claim 11, wherein in the substituted 1,2,4-oxadiazole of general formula I, Y is CH.sub.2.

14. The method of claim 11, wherein the patient is a human.

Description

DESCRIPTION OF THE INVENTION

[0008] The new compounds show a significant activity against Mycobacterium tuberculosis as well as atypical strains, including pathogenic and multiresistant strains isolated from ill patients. They concern namely 1,2,4-oxadiazoles with general formula I

##STR00003## [0009] where Y═S, CH.sub.2; [0010] R=phenyl- or phenyl substituted in positions 2, 3, 4 and 5 by one or several electron-acceptor groups and/or one or several electron-donor groups.

[0011] Electron-donor groups refer to substituents that increase electron density on the R phenyl substituent. They concern in particular: —NH.sub.2, —NHAl, —NAl.sub.2, —OH, —OAl, —OAr, —NHCOCH.sub.3, —NHCOAl; —NHCOAr; —Al, —Ar, where Alk=Alkyl, in particular the one having 1 to 4 carbon atoms, Ar=Aryl, where aryl=phenyl or phenyl substituted in positions 2, 3, 4 and 5 by one or several electron-acceptor groups and/or one or several electron-donor groups, naphthyl or pyridyl.

[0012] Electron-acceptor groups refer to substituents that decrease electron density on the R phenyl substituent. They concern in particular: —NO.sub.2, —N.sup.+AlK.sub.3, —CF.sub.3, CCl.sub.3, —CN, —COOH, —COOAlk, —COOAr, —CHO, —COAlk, —COAr, —F, —Cl, —Br, —I, where Alk=alkyl, in particular the one having 1 to 4 carbon atoms, Ar=Aryl, where Aryl=phenyl or phenyl substituted in positions 2, 3, 4 and 5 by one or several electron-acceptor groups and/or one or several electron-donor groups, naphthyl or pyridyl.

(source: a) John McMurry: Organic Chemistry, Sixth edition, 2004, Brooks/Cole, and Thomson Learning Company; b) L. G. Wade, Jr.: Organic Chemistry, Sixth edition, 2006, Pearson Prentice Hall Inc.; c) J. Clayden, N. Greeves, S. Warren, P. Wothers: Organic Chemistry, 2001, Oxford University Press)

[0013] Another subject of the invention is the application of the aforementioned substituted 1,2,4-oxadiazoles with general formula I according to the invention to be used as antituberculosis drug.

[0014] Another aspect of the invention is a pharmaceutical preparation containing the active substance in the form of 1,2,4-oxadiazole with formula I.

[0015] Compounds with general formula I can be obtained by commonly available procedures known in organic chemistry. During the synthesis of precursor 1,2,4-oxadiazole-5-thiols required for the synthesis of compounds with general formula I, where Y═S, known synthetic methods used in the following publications were employed: a) Xia, G.; You, X.; Liu, L.; Liu, H.; Wang, J.; Shi, Y.; Li, P.; Xiong, B.; Liu, X.; Shen, J., Design, synthesis and SAR of piperidyl-oxadiazoles as 11beta-hydroxysteroid dehydrogenase 1 inhibitors. European Journal of Medicinal Chemistry 2013, 62, 1-10; b) Burns, A. R.; Kerr, J. H.; Kerr, W. J.; Passmore, J.; Paterson, L. C.; Watson, A. J. B., Tuned methods for conjugate addition to a vinyl oxadiazole; Synthesis of pharmaceutically important motifs. Organic and Biomolecular Chemistry 2010, 8 (12), 2777-2783; c) Charton, J.; Cousaert, N.; Bochu, C.; Willand, N.; Deprez, B.; Deprez-Poulain, R. Tetrahedron Letters, 2007, vol. 48, No. 8, p. 1479-1483; d) Astellas Pharma Inc.—EP2511265, 2012, Al. (Scheme 1).

##STR00004##

[0016] Final products with general formula I, where Y═S, were then obtained by Williamson synthesis from relevant 1,2,4-oxadiazole-5-thiol by reaction with commercially available 3,5-dinitrobenzylchloride (Scheme 2). Their preparation is not demanding in terms of synthesis and raw materials required for the preparation thereof are readily available and cheap.

##STR00005##

[0017] Final products with general formula I, where Y═CH.sub.2, were prepared by methods described in references: a) Xia, G.; You, X.; Liu, L.; Liu, H.; Wang, J.; Shi, Y.; Li, P.; Xiong, B.; Liu, X.; Shen, J., Design, synthesis and SAR of piperidyl-oxadiazoles as 11beta-hydroxysteroid dehydrogenase 1 inhibitors. European Journal of Medicinal Chemistry 2013, 62, 1-10; b) Burns, A. R.; Kerr, J. H.; Kerr, W. J.; Passmore, J.; Paterson, L. C.; Watson, A. J. B., Tuned methods for conjugate addition to a vinyl oxadiazole; Synthesis of pharmaceutically important motifs. Organic and Biomolecular Chemistry 2010, 8 (12), 2777-2783.

[0018] Condensation of 3-(3,5-dinitrophenyl)propionic acid with N-hydroxy-imidamide was performed according to standard procedures known in the chemistry of peptides using water-soluble carbodiimide (El-Faham, A.; Albericio, F. Chem. Rev., 2011, 111 (11), pp 6557-6602). The by-product of the reaction (B) was then converted into the final product with general formula I by reaction with sodium methoxide in tetrahydrofuran at the ambient temperature (Scheme 3).

##STR00006##

[0019] The prepared compounds with general formula I were tested by the Institute of Public Health in Ostrava (Department of Bacteriology and Mycology, Laboratory for Diagnostics of Mycobacteria, Partyzanské náměsti 7, 702 00 Ostrava) under in vitro conditions in liquid Sula's medium and their minimum inhibiting concentrations (MIC) were determined as the biological effect of molecules cannot be predicted. The antimycobacterial activity of the prepared compounds was tested on the collection strain Mycobacterium tuberculosis CNCTC My 331/88, collection atypical strains M. avium CNCTC My 330/88 and M. kansasii CNCTC My 235/80. Their activity was compared with the effect of isoniazide (INH), a commonly used medicament. The results of the tests are summarized in Table No. 3.

[0020] The most effective compounds with general formula I were further tested on multiresistant strains of mycobacteria (MDR strains) designated as Praha 1, Praha 4, Praha 131, 9449/2007, 234/2005, 7357/1998 and 8666/2010, which were clinically isolated from patients and are deposited in the Institute of Public Health in Ostrava (Department of Bacteriology and Mycology, Laboratory for Diagnostics of Mycobacteria, Partyzánské náměstí 7, 702 00 Ostrava). The values of sensitivity of these clinically isolated strains to commonly available antituberculosis drugs are summarized in Table No. 4. The antimycobacterial activities of substances with general formula I against these multiresistant strains are summarized in Table No. 5.

[0021] It has been discovered that the aforementioned substances show a highly selective antimycobacterial effect as they do not affect the viability of other types of cells. For all compounds, their cell toxicity was assessed in vitro on mammalian cell lines (the COS-1, HepG2, CHO-K1 cell lines). The respective substances do not affect the viability of mammalian cells up to the concentration of 50 μM. In addition, the respective substances do not affect the viability of standard G+ and G− bacterial strains or fungal strains up to the concentration of 250 μM.

EXAMPLE OF INVENTION EXECUTION

[0022] Hereunder, substituted 1,2,4-oxadiazoles with general formula I will be disclosed

##STR00007##

where the Y, R symbols have the meaning explained above.

Example 1: 5-((3,5-Dinitrobenzyl)sulfanyl)-3-phenyl-1,2,4-oxadiazole (1)

[0023] ##STR00008##

[0024] The compound 5 -((3,5 -dinitrobenzyl)sulfanyl)-3-phenyl-1,2,4-oxadiazole 1 is prepared according to scheme 2 by reaction of 3-phenyl-1,2,4-oxadiazole-5-thiol (0.42 g, 2.34 mmol) with commercially available 3,5-dinitrobenzylchloride (97% purity, 0.47 g, 2.1 mmol) and commercially available triethylamine (0.35 ml, 2.51 mmol) in acetonitrile (30 ml) at the ambient temperature for a period of 12 hours. After the reaction was completed, acetonitrile was distilled off, the residue of evaporation was mixed with a saturated aqueous solution of NaHCO.sub.3 (15 ml) and the solid fraction was filtered off. The acquired solid substance was resuspended in a mixture of diethylether/ethyl-acetate 2:1 (15 ml), filtered off, and the acquired crystalline 5-((3,5-dinitrobenzyl)sulfanyl)-3-phenyl-1,2,4-oxadiazole 1 was dehydrated in a dessicator. The product may be repurified by column chromatography with the use of the mobile phase hexane/ethyl-acetate 5:1.

[0025] The precursor 3-phenyl-1,2,4-oxadiazole-5-thiol was prepared by a known method (Charton, J.; Cousaert, N.; Bochu, C.; Willand, N.; Deprez, B.; Deprez-Poulain, R. Tetrahedron Letters, 2007, vol. 48, No. 8, p. 1479-1483) according to scheme 1. The other substances are generally commercially available.

Example 2: 5-((3,5-Dinitrobenzyl)sulfanyl)-3-(p-tolyl)-1,2,4-oxadiazole (2)

[0026] ##STR00009##

[0027] The compound 5-((3,5-dinitrobenzyl)sulfanyl)-3-(p-tolyl)-1,2,4-oxadiazole 2 is prepared according to scheme 2 by reaction of 3-(4-methylphenyl)-1,2,4-oxadiazole-5-thiol (0.45 g, 2.34 mmol) with commercially available 3,5-dinitrobenzylchloride (97% purity, 0.47 g, 2.1 mmol) and commercially available triethylamine (0.35 ml, 2.51 mmol) in acetonitrile (30 ml) at the ambient temperature for a period of 12 hours. After the reaction was completed, acetonitrile was distilled off, the residue of evaporation was mixed with a saturated aqueous solution of NaHCO.sub.3 (15 ml) and the solid fraction was filtered off. The acquired solid substance was resuspended in a mixture of diethylether/ethyl-acetate 2:1 (15 ml), filtered off, and the acquired crystalline 5-((3,5-dinitrobenzyl)sulfanyl)-3-(p-tolyl)-1,2,4-oxadiazol 2 was dehydrated in a dessicator. The product may be repurified by column chromatography with the use of the mobile phase hexane/ethyl-acetate 5:1.

[0028] The precursor 3-(4-methylphenyl)-1,2,4-oxadiazol-5-thiol was, according to scheme 1, the reaction of commercially available 1,8-diazabicyclo[5.4.0]undec-7-en (2 ml, 2.03 g, 0.0133 mol) with N′-hydroxy-4-methylbenzimidamide (0.5 g, 0.0033 mol) and commercially available 1.1′-thiocarbonyl-diimidazole (0.89 g, 0.005 mol) in tetrahydrofuran (20 ml) under argon atmosphere for a period of 12 hours. The solvent was then distilled off, the raw product was dissolved in water (30 ml) and washed by diethylether (1×30 ml). The aqueous layer was acidified by hydrochloric acid to pH=2 and the product was filtered off and washed by water. The acquired 3-(4-methylphenyl)-1,2,4-oxadiazole-5-thiol was recrystallized from aqueous ethanol.

[0029] The precursor N′-hydroxy-4-methylbenzimidamide was prepared by a known method invented by Murarka, Sandip; Martin-Gago, Pablo; Schultz-Fademrecht, Carsten; Al Saabi, Alaa; Baumann, Matthias; Fansa, Eyad K.; Ismail, Shehab; Nussbaumer, Peter; Wittinghofer, Alfred; Waldmann, Herbert—Chemistry—A European Journal, 2017, vol. 23, #25, p. 6083-6093. The other substances are generally commercially available.

[0030] Using the aforementioned procedures for synthesis, many other compounds with general formula I (compounds 3-10) can be synthesized.

TABLE-US-00001 TABLE 1 Examples of sunbstances with general formula I (compounds 1-10) Y R Compound formula and name  1 S C.sub.6H.sub.5 [00010]  2 S 4-CH.sub.3C.sub.6H.sub.4 [00011]  3 S 4-CH.sub.3OC.sub.6H.sub.4 [00012]  4 S 4-ClC.sub.6H.sub.4 [00013]  5 S 3-ClC.sub.6H.sub.4 [00014]  6 S 2-ClC.sub.6H.sub.4 [00015]  7 S 3,4-Cl.sub.2C.sub.6H.sub.3 [00016]  8 S 4-FC.sub.6H.sub.4 [00017]  9 S 4-BrC.sub.6H.sub.4 [00018] 10 S 4-NO.sub.2C.sub.6H.sub.4 [00019]

Example 3: 5-(3,5-Dinitrophenethyl)-3-phenyl-1,2,4-oxadiazole (11)

[0031] ##STR00020##

[0032] The compound 5-(3,5-dinitrophenethyl)-3-phenyl-1,2,4-oxadiazole 11 is prepared according to scheme 3 by reaction of 3-(3,5-dinitrophenyl)propanoic acid (0.3 g, 1.25 mmol), commercially available 1-hydroxybenzotriazole hydrate (0.71 g, 4.62 mmol), commercially available diisopropylethylamine (0.65 ml, 0.48 g, 3.75 mmol), commercially available N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (0.44 ml, 0.39 g, 2.5 mmol), and N′-hydroxybenzimidamide (0.19 g, 1.38 mmol) in 30 ml of tetrahydrofuran at the boiling temperature for a period of 8 hours. After the completion of the reaction, the solvent was distilled off, the residue of evaporation was dissolved in ethyl-acetate (30 ml) and washed by saturated solution of NaHCO.sub.3 (1×15 ml) and water (1×30 ml). The organic layer was dehydrated by anhydrous Na.sub.2SO.sub.4, evaporated, and the product 5-(3,5-dinitrophenethyl)-3-phenyl-1,2,4-oxadiazole 11 was separated by column chromatography (mobile phase: hexane/ethyl-acetate 5:1). The by-product, N′-((3-(3,5-dinitrophenyl)propanoyl)oxy)benzimidamide was also separated by column chromatography and can be converted into the final product 11 by reaction with 1.5 molar equivalents of sodium methanolate in tetrahydrofuran.

[0033] The precursor 3-(3,5-dinitrophenyl)propanoic acid was prepared by reduction of 3,5-dinitrocinnamic acid by the aforementioned method (Strawn, L. M.; Martell, R. E.; Simpson, R. U.; Leach, K. L.; Counsell R. E. Iodoaryl Analogues of Dioctanoylglycerol and 1-Oleoyl-2-acetylglycerol as Probes for Protein Kinase C, J. Med. Chem. 1989, 32, 2104-2110.) Hydroxylamonnium sulphate (0.96 g, 5.85 mmol) and hydroxylamin-o-sulphonic acid (2.33 g, 20.6 mmol) were added to the suspension of 3,5-dinitrocinnamic acid (0.7 g, 2.94 mmol) in 30 ml of water at 10° C. The value of pH was adjusted to 6-7 by NaOH solution. The reaction mixture was then stirred at the temperature of 10° C. for a period of 5 hours and then at the ambient temperature for a period of 12 hours. After the aforementioned time, pH was adjusted to the value of 8 by NaOH solution, the solution was filtered off and acidified to pH=2 using concentrated HCl. The aqueous solution was then extracted by ethyl-acetate (2×70 ml), the organic extract was washed by water (2×40 ml) and evaporated. 3-(3,5-Dinitrophenyl)propanoic acid was repurified by column chromatography (mobile phase: Hexane/EtOAc/CH.sub.3COOH, 50:10:1). 3,5-Dinitrocinnamic acid was prepared from commercially available 3,5-dinitro-benzaldehyde according to the following procedure: malonic acid (0.8 g, 7.67 mmol) and 0.1 ml piperidine was added to the solution of 3,5-dinitrobenzaldehyde (1 g, 5.1 mmol) in 10 ml of pyridine. The reaction mixture was heated up to the boiling temperature of the solvent for a period of 6 hours. Then the reaction mixture was poured into 100 ml of 2M solution of HCl. The resulting precipitate was filtered off and dissolved in 70 ml of ethyl-acetate. The organic solution was washed by water (2×40 ml), dried above Na.sub.2SO.sub.4 and evaporated. 3,5-Dinitrocinnamic acid was repurified by column chromatography (mobile phase: Hexane/EtOAc/CH.sub.3COOH, 40:10: 1).

[0034] The precursor N′-hydroxybenzimidamide was prepared by a known method (Burns, A. R.; Kerr, J. H.; Kerr, W. J.; Passmore, J.; Paterson, L. C.; Watson, A. J. B., Tuned methods for conjugate addition to a vinyl oxadiazole; Synthesis of pharmaceutically important motifs. Organic and Biomolecular Chemistry 2010, 8 (12), 2777-2783.).

Example 4:5-(3,5-Dinitrophenethyl)-3-(p-tolyl)-1,2,4-oxadiazole (12)

[0035] ##STR00021##

[0036] The compound 5-(3,5-dinitrofenethyl)-3-(p-tolyl)-1,2,4-oxadiazol 12 is prepared according to scheme 3 by reaction of 3-(3,5-dinitrophenyl)propanoic acid (0.3 g, 1.25 mmol), commercially available 1-hydroxybenzotriazole hydrate (0.71 g, 4.62 mmol), commercially available diisopropylethylamine (0.65 ml, 0.48 g, 3.75 mmol), commercially available N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (0.44 ml, 0.39 g, 2.5 mmol), and N′-hydroxy-4-methylbenzimidamide (0.206 g, 1.38 mmol) in 30 ml of tetrahydrofuran at the boiling temperature of the solvent for a period of 8 hours. After the completion of the reaction, the solvent was distilled off, the residue of evaporation was dissolved in ethyl-acetate (30 ml) and washed by saturated solution of NaHCO.sub.3 (1×15 ml) and water (1×30 ml). The organic layer was dehydrated by anhydrous Na.sub.2SO.sub.4, evaporated, and the product 5-(3,5-dinitrophenethyl)-3-(p-tolyl)-1,2,4-oxadiazole 12 was separated by column chromatography (mobile phase: hexane/ethyl-acetate 5:1). The by-product, N′-((3-(3,5-dinitrophenyl)propanoyl)oxy)-4-methylbenzimidamide was also separated by column chromatography and can be converted into the final product 12 by reaction with 1.5 molar equivalents of sodium methanolate in tetrahydrofuran.

[0037] The precursor 3-(3,5-dinitrophenyl)propanoic acid was prepared by reduction of 3,5-dinitrocinnamic acid by the aforementioned method (Strawn, L. M.; Martell, R. E.; Simpson, R. U.; Leach, K. L.; Counsell R. E. Iodoaryl Analogues of Dioctanoylglycerol and 1-Oleoyl-2-acetylglycerol as Probes for Protein Kinase C, J. Med. Chem. 1989, 32, 2104-2110.) Hydroxylamonnium sulphate (0.96 g, 5.85 mmol) and hydroxylamin-o-sulphonic acid (2.33 g, 20.6 mmol) were added to the suspension of 3,5-dinitrocinnamic acid (0.7 g, 2.94 mmol) in 30 ml of water at 10° C. The value of pH was adjusted to 6-7 by NaOH solution. The reaction mixture was then stirred at the temperature of 10° C. for a period of 5 hours and then at the ambient temperature for a period of 12 hours. After the aforementioned time, pH was adjusted to the value of 8 by NaOH solution, the solution was filtered off and acidified to pH=2 using concentrated HCl. The aqueous solution was then extracted by ethyl-acetate (2×70 ml), the organic extract was washed by water (2 x 40 ml) and evaporated. 3-(3,5-Dinitrophenyl)propanoic acid was repurified by column chromatography (mobile phase: Hexane/EtOAc/CH.sub.3COOH, 50:10:1). 3,5-Dinitrocinnamic acid was prepared from commercially available 3,5-dinitrobenzaldehyde according to the following procedure: malonic acid (0.8 g, 7.67 mmol) and 0.1 ml piperidine was added to the solution of 3,5-dinitrobenzaldehyde (1 g, 5.1 mmol) in 10 ml of pyridine. The reaction mixture was heated up to the boiling temperature of the solvent for a period of 6 hours. Then the reaction mixture was poured into 100 ml of 2M solution of HCl. The resulting precipitate was filtered off and dissolved in 70 ml of ethyl-acetate. The organic solution was washed by water (2×40 ml), dried above Na.sub.2SO.sub.4 and evaporated. 3,5-Dinitrocinnamic acid was repurified by column chromatography (mobile phase: Hexane/EtOAc/CH.sub.3COOH, 40:10:1).

[0038] The precursor N′-hydroxy-4-methylbenzimidamide was prepared by a known method based on the following publication: Murarka, Sandip; Martin-Gago, Pablo; Schultz-Fademrecht, Carsten; Al Saabi, Alaa; Baumann, Matthias; Fansa, Eyad K.; Ismail, Shehab; Nussbaumer, Peter; Wittinghofer, Alfred; Waldmann, Herbert—Chemistry—A European Journal, 2017, vol. 23, #25, p. 6083-6093.

[0039] Using the aforementioned procedures for synthesis, many other compounds with general formula I (compounds 13-16) can be synthesized.

TABLE-US-00002 TABLE 2 Examples of substances with general formula I (compounds 11-16) Y R Compound formula and name 11 CH.sub.2 C.sub.6H.sub.5 [00022] 12 CH.sub.2 4-CH.sub.3C.sub.6H.sub.4 [00023] 13 CH.sub.2 4-CH.sub.3OPh [00024] 14 CH.sub.2 4-ClPh [00025] 15 CH.sub.2 3,4-Cl.sub.2Ph [00026] 16 CH.sub.2 4-FPh [00027]

TABLE-US-00003 TABLE 3 Minimum inhibiting concentration (μmol .Math. l.sup.−1) in vitro of substances with general formula I - the micromethod for determination of minimum inhibiting concentrations of medicines in Sula's medium in plastic P-plates, after 14 and 21 days of incubation for M. tuberculosis and M. avium and after 7, 14, and 21 days of incubations for M. kansasii. M. tuberculosis M. avium M. kansasii My 331/88 My 330/88 My 235/80 1 0.5/1.sup.  250/250 0.5/1/1 2 0.5/1.sup.  250/250 0.5/1/2 3 0.5/0.5 250/250 0.5/1/2 4 0.5/1.sup.  250/250 1/2/4 5 0.5/1.sup.  250/250 2/2/4 6 0.5/1.sup.  125/125 8/16/32 7 1/1 250/250 1/1/2 8 0.25/0.5  250/250 0.5/1/2 9 1/1 125/125 1/1/2 10 0.5/1.sup.  250/250 2/2/4 11 4/4 250/250 2/4/8 12 2/4 250/250 2/4/4 13 0.5/1.sup.  250/250 0.5/1/2 14 >32/>32 250/250 >32/>32/>32 15 >32/>32 250/250 32/>32/>32 16 >32/>32 250/250 >32/>32/>32

TABLE-US-00004 TABLE 4 Minimum inhibiting concentrations (μmol .Math. l.sup.−1) in vitro of commonly used antibiotics and antituberculosis medicaments - the micromethod for determination of minimum inhibiting concentrations of medicines in Sula's medium in plastic P-plates - for multiresistant strains M. tuberculosis. M. tuberculosis Praha 1 Praha 4 Praha 131 9449/2007 234/2005 7357/1998 8666/2010 Streptomycin 13.7 R >27.5 R >27.5 R >27.5 R 27.5 R >27.5 R >27.5 R Isoniazid 14.6 R 14.6 R 14.6 R 58.3 R 14.6 R 14.6 R 29.2 R Etambutol 39.2 R 19.6 R 39.2 R 9.8 C 19.6 R 19.6 R 19.6 R Rifampicin >9.7 R >9.7 R >9.7 R >9.7 R >9.7 R >9.7 R >9.7 R Ofloxacin 1.38 C >22.2 R 22.2 R 2.75 C 0.69 C 11.1 R 11.1 R Gentamicin 1.05 C 0.52 C >8.37 R 1.05 C 0.26 C 1.05 C 2.09 C Clofazimin 0.53 R 0.53 R 0.26 C 0.13 C 0.06 C 0.13 C 2.11 R Amikacin 0.43 C 0.85 C >27.2 R 0.43 C 0.43 C 0.85 C 1.7 C R - strain resistant to the given antituberculosis drug C - strain sensitive to the given antituberculosis drug

TABLE-US-00005 TABLE 5 Minimum inhibiting concentrations (μmol .Math. l.sup.−1) in vitro of substances with general formulation I - the micromethod for determination of minimum inhibiting concentrations of medicines in Sula's medium in plastic P-plates, after 14 days of incubation - for multiresistant strains M. tuberculosis. M. tuberculosis (MDR strains) Praha 1 Praha 4 Praha 131 9449/2007 234/2005 7357/1998 8666/2010 9 0.5 0.5 1 0.5 0.5 1 0.5 10 1 0.5 1 0.5 0.5 1 0.5 5 1 0.5 1 1 0.5 1 0.5 4 2 0.5 1 1 0.5 1 0.5 3 1 0.5 0.5 0.5 0.5 0.5 0.5 8 0.5 0.5 0.5 0.5 0.5 0.5 0.5

TABLE-US-00006 TABLE 6 Melting points and NMR spectra of substances with general formula I Melting point [° C.] .sup.1H NMR .sup.13C NMR 1 135-136° C. .sup.1H NMR (500 MHz, Acetone) δ .sup.13C NMR (126 MHz, Acetone) δ 9.06-9.00 (m, 2H), 8.90-8.84 (m, 178.26, 169.11, 149.35, 142.65, 1H), 8.12-8.01 (m, 2H), 7.64-7.50 132.51, 130.89, 129.94, 128.10, (m, 3H), 5.03-4.93 (m, 2H). 127.07, 118.81, 35.85. .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ .sup.13C NMR (126 MHz, DMSO-d.sub.6) δ 8.94 (d, J = 2.1 Hz, 2H), 8.73 (t, J = 177.57, 167.90, 148.01, 141.57, 2.1 Hz, 1H), 8.03-7.97 (m, 2H), 132.08, 130.25, 129.41, 127.23, 7.64-7.53 (m, 3H), 4.89 (s, 2H). 125.66, 118.12, 34.74. 2 150-152° C. .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ .sup.13C NMR (126 MHz, DMSO-d.sub.6) δ 8.93 (d, J = 1.8 Hz, 2H), 8.72 (t, J = 177.33, 167.85, 147.99, 142.12, 1.8 Hz, 1H), 7.89 (d, J = 8.0 Hz, 2H), 141.59, 130.25, 129.94, 127.16, 7.36 (d, J = 8.0 Hz, 2H), 4.88 (s, 122.86, 118.09, 34.71, 21.23. 2H), 2.38 (s, 3H). 3 155-156° C. .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ .sup.13C NMR (126 MHz, DMSO-d.sub.6) δ 8.92 (d, J = 2.2 Hz, 2H), 8.76-8.56 177.11, 167.63, 162.13, 148.01, (m, 1H), 7.93 (d, J = 8.5 Hz, 2H), 141.64, 130.21, 128.97, 118.10, 7.09 (d, J = 8.5 Hz, 2H), 4.87 (s, 117.87, 114.80, 55.62, 34.68. 2H), 3.84 (s, 3H). 4 173-175° C. .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ .sup.13C NMR (126 MHz, DMSO-d.sub.6) δ 8.93 (d, J = 2.2 Hz, 2H), 8.73 (t, J = 177.87, 167.15, 148.04, 141.50, 2.2 Hz, 1H), 8.01 (d, J = 8.5 Hz, 2H), 136.84, 130.22, 129.60, 129.03, 7.64 (d, J = 8.5 Hz, 2H), 4.89 (s, 124.55, 118.14, 34.74. 2H). 5 133-135° C. .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ .sup.13C NMR (126 MHz, DMSO-d.sub.6) δ 8.93 (d, J = 2.1 Hz, 2H), 8.72 (t, J = 178.07, 166.85, 147.98, 141.51, 2.1 Hz, 1H), 7.97 (t, J = 1.9 Hz, 1H), 134.19, 131.91, 131.40, 130.26, 7.93 (dt, J = 1.1, 1.3 Hz, 1H), 7.67 127.62, 126.85, 125.73, 118.06, (ddd, J = 8.1, 2.2, 1.1 Hz, 1H), 7.58 34.75. (t, J = 7.9 Hz, 1H), 4.88 (s, 2H). 6 158-160° C. .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ .sup.13C NMR (126 MHz, DMSO-d.sub.6) δ 8.89 (d, J = 2.2 Hz, 2H), 8.74 (t, J = 177.24, 166.81, 148.06, 141.61, 2.2 Hz, 1H), 7.94 (dd, J = 7.8, 1.7 133.03, 132.30, 131.90, 131.05, Hz, 1H), 7.67 (dd, J = 8.1, 1.3 Hz, 130.11, 127.78, 124.93, 118.12, 1H), 7.61 (td, J = 7.7, 1.7 Hz, 1H), 34.82. 7.53 (td, J = 7.6, 1.4 Hz, 1H), 4.89 (s, 2H). 7 176-177° C. .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ .sup.13C NMR (126 MHz, DMSO-d.sub.6) δ 8.95 (d, J = 2.1 Hz, 2H), 8.73 (t, J = 178.61, 166.58, 148.31, 141.75, 2.1 Hz, 1H), 8.19 (d, J = 2.0 Hz, 1H), 135.18, 132.76, 132.18, 130.60, 7.96 (dd, J = 8.4, 2.0 Hz, 1H), 7.85 129.32, 127.47, 126.49, 118.42, (d, J = 8.4 Hz, 1H), 4.90 (s, 2H). 35.04. 8 150-152° C. .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ .sup.13C NMR (126 MHz, DMSO-d.sub.6) δ 8.92 (d, J = 2.1 Hz, 2H), 8.72 (t, J = 177.70, 167.13, 164.30 (d, J = 2.1 Hz, 1H), 8.07-8.01 (m, 2H), 249.9 Hz), 148.02, 141.56, 130.21, 7.43-7.36 (m, 2H), 4.88 (s, 2H). 129.80 (d, J = 9.1 Hz), 122.25 (d, J = 3.0 Hz), 118.10, 116.60 (d, J = 22.3 Hz), 34.73. 9 175-176° C. .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ .sup.13C NMR (126 MHz, DMSO-d.sub.6) δ 8.92 (d, J = 2.2 Hz, 2H), 8.72 (t, J = 177.86, 167.25, 148.02, 141.48, 2.1 Hz, 1H), 7.93 (d, J = 8.5 Hz, 2H), 132.50, 130.20, 129.15, 125.70, 7.77 (d, J = 8.4 Hz, 2H), 4.89 (s, 124.88, 118.11, 34.74. 2H). 10 184-185° C. .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ .sup.13C NMR (126 MHz, DMSO-d.sub.6) δ 8.94 (d, J = 2.1 Hz, 2H), 8.73 (t, J = 178.50, 166.68, 149.54, 148.07, 2.1 Hz, 1H), 8.39 (d, J = 8.5 Hz, 2H), 141.39, 131.48, 130.21, 128.65, 8.26 (d, J = 8.5 Hz, 2H), 4.92 (s, 124.57, 118.15, 34.79. 2H). 11 120-122° C. .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ .sup.13C NMR (126 MHz, DMSO-d.sub.6) δ 8.71 (d, J = 2.0 Hz, 2H), 8.71-8.69 179.29, 167.63, 148.13, 144.58, (m, 1H), 8.00-7.96 (m, 2H), 7.60- 131.73, 129.75, 129.42, 127.10, 7.52 (m, 3H), 3.52-3.40 (m, 4H). 126.33, 117.00, 30.64, 26.84. 12 147-148° C. .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ .sup.13C NMR (126 MHz, DMSO-d.sub.6) δ 8.71 (d, J = 2.1 Hz, 2H), 8.70-8.68 179.05, 167.57, 148.10, 144.59, (m, 1H), 7.86 (d, J = 8.2 Hz, 2H), 141.65, 129.93, 129.72, 127.02, 7.35 (d, J = 8.2 Hz, 2H), 3.50-3.38 123.54, 116.95, 30.63, 26.82, (m, 4H), 2.37 (s, 3H). 21.20. 13 128-129° C. .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ .sup.13C NMR (126 MHz, DMSO-d.sub.6) δ 8.73-8.67 (m, 3H), 7.91 (d, J = 8.8 178.90, 167.32, 161.86, 148.12, Hz, 2H), 7.09 (d, J = 8.8 Hz, 2H), 144.61, 129.72, 128.78, 118.59, 3.83 (s, 3H), 3.46-3.42 (m, 4H). 116.97, 114.80, 55.56, 30.66, 26.80. 14 182-183° C. .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ .sup.13C NMR (126 MHz, DMSO-d.sub.6) δ 8.70 (s, 3H), 7.99 (d, J = 8.5 Hz, 179.57, 166.87, 148.15, 144.53, 2H), 7.63 (d, J = 8.5 Hz, 2H), 3.51- 136.48, 129.73, 129.64, 128.91, 3.42 (m, 4H). 125.19, 117.02, 30.61, 26.84. 15 154-155° C. .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ .sup.13C NMR (126 MHz, DMSO-d.sub.6) δ 8.74-8.68 (m, 3H), 8.14-8.11 (m, 179.90, 166.00, 148.13, 144.48, 1H), 7.97-7.92 (m, 1H), 7.86-7.82 134.54, 132.37, 131.96, 129.80, (m, 1H), 3.53-3.39 (m, 4H). 128.76, 127.16, 126.82, 117.02, 30.53, 26.82. 16 169-171° C. .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ .sup.13C NMR (126 MHz, DMSO-d.sub.6) δ 8.73-8,60 (m, 3H), 8.05-7.98 (m, 179.40, 166.84, 164.11 (d, J = 2H), 7.42-7.31 (m, 2H), 3.51-3.40 249.4 Hz), 148.13, 144.55, 129.72, (m, 4H). 129.62 (d, J = 9.0 Hz), 122.89 (d, J = 3.0 Hz), 116.98, 116.59 (d, J = 22.1 Hz), 30.63, 26.82.

TABLE-US-00007 TABLE 9 Elementary analysis of substances with general formula I quantified measured 1 C, 50.28; H, 2.81; N, 15.64; S, 8.95 C, 50.45; H, 2.68; N, 15.52; S, 9.11 2 C, 51.61; H, 3.25; N, 15.05; S, 8.61 C, 51.78; H, 3.33; N, 14.96; S, 8.58 3 C, 49.48; H, 3.11; N, 14.43; S, 8.26 C, 49.67; H, 3.25; N, 14.24; S, 8.19 4 C, 45.87; H, 2.31; N, 14.26; S, 8.16 C, 45.82; H, 2.36; N, 14.18; S, 8.12 5 C, 45.87; H, 2.31; N, 14.26; S, 8.16 C, 45.62; H, 2.48; N, 14.61; S, 7.96 6 C, 45.87; H, 2.31; N, 14.26; S, 8.16 C, 45.98; H, 2.40; N, 14.37; S, 8.41 7 C, 42.17; H, 1.89; N, 13.11; S, 7.50 C, 42.02; H, 2.03; N, 13.0; S, 7.63 8 C, 47.88; H, 2.41; N, 14.89; S, 8.52 C, 44.01; H, 2.34; N, 14.97; S, 8.43 9 C, 41.21; H, 2.07; N, 12.81; S, 7.33 C, 41.39; H, 2.14; N, 12.72; S, 7.21 10 C, 44.67; H, 2.25; N, 17.36; S, 7.95 C, 44.91; H, 2.36; N, 17.27; S, 7.79 11 C, 56.47; H, 3.55; N, 16.46 C, 56.69; H, 3.16; N, 16.39 12 C, 57.63; H, 3.98; N, 15.81 C, 57.49; H, 3.7; N, 15.85 13 C, 55.14; H, 3.81; N, 15.13 C, 55.19; H, 3.83; N, 15.07 14 C, 51.28; H, 2.96; N, 14.95 C, 51.61; H, 2.84; N, 14.9 15 C, 46.97; H, 2.46; N, 13.69 C, 46.81; H, 2.38; N, 13.62 16 C, 53.64; H, 3.09; N, 15.64 C, 53.51; H, 3.03; N, 15.76

Examples of Pharmaceutical Products—Tablets

[0040] In production of pharmaceutical forms, the technology usually employed in this field is opted for, i.e. dry or wet granulation, that is known to persons skilled in the art. Common and well-proven excipients and suitable agents providing the pharmaceutical form with required physical properties are used.

Examples for Dry Granulation

Example 1 (the content of active substance 100 mg)

[0041]

TABLE-US-00008 Active substance with general formula I 1 100.0 mg Microcrocrystalline cellulose 75.0 mg Sodium starch glycolate 3.5 mg Magnesium stearate 0.5 mg Silicone dioxide, colloidal 0.5 mg

Example 2 (the content of active substance 200 mg)

[0042]

TABLE-US-00009 Active substance with general formula I 6 200.0 mg Microcrocrystalline cellulose 95.0 mg Sodium starch glycolate 7.0 mg Magnesium stearate 1.0 mg Silicone dioxide, colloidal 1.0 mg

Example 3 (the content of active substance 300 mg)

[0043]

TABLE-US-00010 Active substance with general formula I 16 300.0 mg Microcrocrystalline cellulose 115.0 mg Sodium starch glycolate 10.5 mg Magnesium stearate 1.5 mg Silicone dioxide, colloidal 1.5 mg

Example 4 (the content of active substance 400 mg)

[0044]

TABLE-US-00011 Active substance with general formula I 12 400.0 mg Microcrocrystalline cellulose 130.0 mg Sodium starch glycolate 14.5 mg Magnesium stearate 2.0 mg Silicone dioxide, colloidal 2.0 mg

Example 5 (the content of active substance 500 mg)

[0045]

TABLE-US-00012 Active substance with general formula I 9 500.0 mg Microcrocrystalline cellulose 140.0 mg Sodium starch glycolate 17.5 mg Magnesium stearate 2.5 mg Silicone dioxide, colloidal 2.5 mg

[0046] Active substance is mixed with individual ingredients of the tablet material and the mixture is transformed into tables on a tablet making machine in a customary method.

Examples for Wet Granulation

Example 6 (the content of active substance 100 mg)

[0047]

TABLE-US-00013 Active substance with general formula I 13 100.0 mg Potato starch 48.0 mg Lactose 27.0 mg Polyvinylpyrrolidon 3.0 mg Sodium starch glycolate 4.0 mg Magnesium stearate 0.2 mg Talc 1.8 mg

Example 7 (the content of active substance 200 mg)

[0048]

TABLE-US-00014 Active substance with general formula I 4 200.0 mg Potato starch 60.8 mg Lactose 34.2 mg Polyvinylpyrrolidon 6.0 mg Sodium starch glycolate 8.0 mg Magnesium stearate 0.4 mg Talc 3.6 mg

Example 8 (the content of active substance 300 mg)

[0049]

TABLE-US-00015 Active substance with general formula I 11 300.0 mg Potato starch 73.6 mg Lactose 41.4 mg Polyvinylpyrrolidon 9.0 mg Sodium starch glycolate 12.0 mg Magnesium stearate 0.6 mg Talc 5.4 mg

Example 9 (the content of active substance 400 mg)

[0050]

TABLE-US-00016 Active substance with general formula I 2 400.0 mg Potato starch 82.3 mg Lactose 46.8 mg Polyvinylpyrrolidon 12.0 mg Sodium starch glycolate 16.0 mg Magnesium stearate 0.8 mg Talc 7.2 mg

Example 10 (the content of active substance 500 mg)

[0051]

TABLE-US-00017 Active substance with general formula I 1 500.0 mg Potato starch 96.0 mg Lactose 54.0 mg Polyvinylpyrrolidon 15.0 mg Sodium starch glycolate 20.0 mg Magnesium stearate 1.0 mg Talc 9.0 mg

[0052] Active substance is gradually mixed with lactose, potato starch, the mixture is granulated by polyvinylpyrrolidon, the dried granulate is mixed with sodium starch glycolate, magnesium stearate and talc and the resulting mixture is formed into tablets in a tablet making machine in a usual method.

APPLICABILITY IN INDUSTRY

[0053] New antituberculosis drugs based on nitro-substituted 1,2,4-oxadiazole compounds effective against sensitive as well as multiresistant mycobacterial strains.