CLINICAL-GRADE AUTOLOGOUS BRONCHIAL BASAL CELL, DELIVERABLE FORMULATION, AND PREPARATION PROCESS

Abstract

Provided are a clinical-grade autologous bronchial basal cell, a deliverable formulation thereof, and a preparation process. The preparation process comprises the following steps: acquiring and digesting an in vitro active bronchoscopic brushed-off biopsy tissue, and collecting cells after digestion is terminated; plating the digested cells on a culture plate pre-coated with feeder layer cells, collecting the cultured cells to perform amplification culture on a culture plate pre-coated with feeder layer cells, and when the cells grow to cover 50%-90% of the surface area of the culture plate, performing cell passage operation; and when passaged culture cells grow to cover 85%-95% of the surface area of a culture dish, digesting and collecting adhered cells, and washing. The clinical-grade bronchial basal cell prepared by this method can differentiate stably after the cells reach the lesion and significantly achieve the damaged lung tissue regeneration.

Claims

1. A preparation process of clinical-grade autologous bronchial basal cells, comprising the following steps: tissue preparation: getting an ex vivo bronchoscopic brushed-off biopsy tissue; enzymolysis: digesting the bronchoscopic brushed-off biopsy tissue, and collecting digested cells after terminating the digesting; plating and amplification culture: getting part of the digested cells to perform primary culture by plating the cells on a culture plate pre-coated with feeder layer cells, collecting the primary cultured cells to perform amplification culture on a culture plate pre-coated with feeder layer cells, followed by passage culture when the cells grow to cover 50%-90% of the surface area of the culture plate; cell collecting: when passage cells grow to cover 85%-95% of the surface area of the culture plate, digesting and collecting adherent cells, and washing.

2. The preparation process according to claim 1, wherein in the step of enzymolysis, the digesting is performed at a temperature of 37° C. for a period of 0.5-2 h, with a tissue digestion solution comprising 99 v % of DMEM/F12, 1-20 ng/ml of DNase, 0.1-4 mg/ml of protease Type XIV, and 10-200 ng/ml of trypsin.

3. The preparation process of claim 1, wherein in the step of enzymolysis, said terminating the digesting is performed with a stop buffer comprising 90 v % of DMEM and 10 v % of fetal bovine serum.

4. The preparation process according to claim 1, wherein the bronchoscopic brushed-off biopsy tissue is taken from at least one site of the lung lobe.

5. The preparation process according to claim 1, wherein the digested cells yielded in the step of enzymolysis and the primary cultured cells are put into frozen storage; preferably before the frozen storage, bacterial detection and mycoplasma detection are carried out.

6. The preparation process according to claim 5, wherein the frozen storage cells are thawed and used to perform the primary culture and/or amplification culture.

7. The preparation process according to claim 1, wherein the primary culture comprises the following steps: re-suspending the cells to be primary cultured in a culture medium and plating the resulting suspension on a culture plate pre-coated with feeder layer cells, and adding antibiotics into the culture plate; performing culture at 37° C., 4-8% CO.sub.2, replacing the culture medium every other day, and collecting the cells when the cells are grown to form clones and further form colonies wherein 80% or more of the clones contain 40-100 cells and clones of grade A and grade B are observed in three fields of view among randomly selected five fields of view.

8. The preparation process according to claim 1, wherein said amplification culture on a culture plate pre-coated with feeder layer cells is performed at 37° C., and meanwhile morphology of the cells is observed and detected to ensure clones of grade A and clones of grade B exceed 50% in randomly observed five fields of view; and wherein in said culture plate pre-coated with feeder layer cells, 50-70% of the culture plate area is pre-coated with feeder layer cells, and wherein the clones of grade A have a regular and smooth outline, and the clones show clear boundary, and cells in the clones are closely packed and have uniform size; and wherein the clones of grade B have a basically regular outline, and the clones show smooth and clear boundary, and in the clones most of the cells are closely packed and have uniform size but a small amount of the cells have a slightly larger size and are slightly loose packed.

9. (canceled)

10. The preparation process according to claim 1, wherein the passage culture comprises n passages (1<n≤7), wherein n-1.sup.th passages are carried out with a culture dish pre-coated with feeder layer cells, and when the cells are grown to cover 50-90% of the culture dish surface, the n.sup.th passage is performed with a culture dish pre-coated with matrigel.

11. The preparation process according to claim 10, wherein each passage of said n passages grows to cover 50%-90% of the culture dish surface.

12. The preparation process according to claim 10, wherein the passage culture comprises: 1.sup.st passage: culturing the cells with a culture dish pre-coated with feeder layer cells, removing supernatant when the cells are grown to cover 50%-90% of the culture dish surface, washing once with 1×DPBS, adding trypsin for digesting, pipetting up and down to prepare a single-cell suspension when most of the cells become round and bright, terminating the digesting with a stop buffer, collecting cell suspension to perform centrifuging and remove supernatant, re-suspending the cells in a culture medium, and finally plating the suspension into a culture dish pre-coated with feeder layer cells, and replacing the culture medium every other day; 2.sup.nd to n-1.sup.th passages: when the last passage grows to cover 50%-90% of the culture dish surface, removing culture supernatant, washing once with 1×DPBS, adding trypsin for digesting, pipetting up and down to prepare a single-cell suspension when most of the cells become round and bright, terminating the digesting with a stop buffer, collecting cell suspension to perform centrifuging and remove supernatant, re-suspending the cells in a culture medium, and finally plating the suspension into a culture dish pre-coated with feeder layer cells, and replacing the culture medium every other day; and n.sup.th passage: when the last passage grows to cover 50%-90% of the culture dish surface, removing culture supernatant, washing once with 1×DPBS, adding trypsin and 1×DPBS to perform digesting at 37° C. for 1-2min, detaching the feeder layer cells by pipetting up and down, again washing once with 1×DPBS, again adding trypsin for digesting, detaching adherent cells by pipetting up and down to prepare a single-cell suspension when most of the cells become round and bright, terminating the digesting with a stop buffer, collecting cell suspension to perform centrifuging and remove supernatant, re-suspending the cells in a cultural medium, and finally plating the suspension into a culture dish pre-coated with matrigel, and replacing the culture medium every other day.

13. The preparation process according to claim 10, wherein after n-2.sup.th passages, 1.1 ml of culture supernatant and 3-16×10.sup.5 cells are collected to carry out tests, wherein bacterial detection and mycoplasma detection give negative results, and biological effect test gives a HOPX positive cell percentage of ≥30%, and cell identification test confirms cells are KRT5 positive and purity analysis shows KRT5 cells percentage is ≥90%; and after n-1.sup.th passages, 0.8 ml of culture supernatant is taken to carry out endotoxin content detection and streptomycin residue detection, and the results show that endotoxin content <0.125 Eu/mL, and streptomycin residue <4 ppb.

14. The preparation process according to claim 7, wherein the culture medium has a formulation consisting of: 225 mL of DMEM, 225 mL of F12, 20-70 mL of FBS, 0.2-2 mM of L-glutamine, 1-14 ng/mL of insulin, 0.1-1 ng/mL of epidermal growth factor, 5-30 μg/mL adenine, and 2-20 μg/mL hydrocortisone.

15. Clinical-grade autologous bronchial basal cells prepared by the preparation process of claim 1.

16. A deliverable formulation of clinical-grade autologous bronchial basal cells, comprising the clinical-grade autologous bronchial basal cells of claim 15 and a clinical-grade injection liquid.

17. A preparation process of a deliverable formulation of clinical-grade autologous bronchial basal cells, comprising the following steps: washing cells produced by amplification culture for 2-4 times with 1×DPBS, re-suspending the cells in 1×DPBS after the last washing, followed by centrifuging, discarding the supernatant, re-suspending the cell pellet, adding a clinical-grade injection liquid to reach a cell concentration of 1-22×10.sup.6 cells/mL, mixing well, and taking 1 ml of the resulting preparation as a reserved sample.

18. The preparation process according to claim 17, wherein the clinical-grade injection liquid is normal saline for injection.

19. The preparation process according to claim 17, wherein after said re-suspending the cells in 1×DPBS, 10 μL of the resulting suspension is taken for trypan blue staining, showing ≥90% cells are alive.

20. The preparation process according to claim 12, wherein the culture medium has a formulation consisting of: 225 mL of DMEM, 225 mL of F12, 20-70 mL of FBS, 0.2-2 mM of L-glutamine, 1-14 ng/mL of insulin, 0.1-1 ng/mL of epidermal growth factor, 5-30 μg/mL adenine, and 2-20 μg/mL hydrocortisone.

21. A method for treating a disease selected from the group consisting of bronchiectasis, emphysema, chronic obstructive pulmonary disease, chronic bronchitis and interstitial lung disease, comprising administering the deliverable formulation of clinical-grade autologous bronchial basal cells of claim 16 to a subject in need.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0054] In order to more clearly illustrate the embodiments of the present application and the prior art, the drawings used in the embodiments and the prior art will be briefly described below. Obviously, the drawings attached in the following description only represent some examples of the present application, and those skilled in the art can obtain other drawings based on these drawings without any creative intellectual work.

[0055] FIG. 1 provides a flow chart showing preparation process of deliverable formulation of clinical-grade autologous bronchial basal cells in Example 4 of the present application;

[0056] FIG. 2 provides a photograph showing morphology of cell clones in Example 4 of the present application;

[0057] FIG. 3 provides photographs showing typical morphology of cell clones of different grades in Example 4 of the present application, wherein Figure A shows morphology of cell clones of grade A, and Figure B shows morphology of cell clones of grade B;

[0058] FIG. 4 provide images showing HR-CT results of a bronchiectasis patient before and after application of the deliverable formulation of clinical-grade autologous bronchial basal cells in Experimental Example 2 of the present application;

[0059] FIG. 5 provides images showing CT results (bullae of the lung) of a patient with interstitial lung disease before and after application of the deliverable formulation of clinical-grade autologous bronchial basal cells in Experimental Example 4 of the present application;

[0060] FIG. 6 provides images showing CT results (destroyed lung) of a patient with interstitial lung disease before and after application of the deliverable formulation of clinical-grade autologous bronchial basal cells in Experimental Example 4 of the present application.

DETAILED DESCRIPTION

[0061] In the present application, FBS represents fetal bovine serum;

[0062] DMEM represents a high glucose DMEM medium;

[0063] F12 represents an F12 nutrient mixture;

[0064] DPBS represents Dulbecco's Phosphate Buffered Saline;

[0065] HOPX represents a marker of the mature, functional lung cells;

[0066] KRT5 represents a marker of bronchial basal cells.

Example 1

[0067] Provided is a preparation process of clinical-grade autologous bronchial basal cells, comprising the following steps:

[0068] An ex vivo bronchoscopic brushed-off biopsy tissue is selected. Particularly tissues from two different sites of bronchus are selected in this example. Selecting tissues from two or more different sites of bronchus can increase the success rate of separation.

[0069] Tissue digestion solution and stop buffer are prepared for later use. The tissue digestion solution comprises 99 v % of DMEM/F12, and remaining is: 1-20 ng/mL of DNase, 0.1-4 mg/mL of protease Type XIV, and 10-200 ng/mL of trypsin. The stop buffer comprises 90 v % of DMEM and 10 v % FBS.

[0070] The bronchoscopic brushed-off biopsy tissue is digested with the tissue digestion solution. The digestion is terminated with a stop buffer, and digested cells are collected.

[0071] A culture medium is prepared for later use, wherein the culture medium comprises 225 mL of DMEM, 225 mL of F12, 20-70 mL of FBS, 0.2-2 mM L-glutamine, 1-14 ng/mL of insulin, 0.1-1 ng/mL epidermal growth factor, 5-30 μg/mL adenine, and 2-20 μg/mL hydrocortisone.

[0072] A culture plate pre-coated with feeder layer cells is prepared for later use. The preparation of the plate is as follows: 20% matrigel is firstly added to coat the culture plate and overspread the bottom surface of the culture plate, and excess liquid is sucked out. Then the culture plate is placed at 37° C. for 15 minutes, thereafter irradiation-inactivated feeder layer cells are plated and cultured in a cell incubator (37° C., 5% CO.sub.2). After 1-2 days, the plate is ready for use if the cells spread 50-70% of the bottom surface of the culture plate.

[0073] A part of the digested cells are taken, re-suspended in the culture medium and plated into one well of a 6-well culture plate pre-plated with the feeder layer cells. 200 μL of penicillin-streptomycin solution (10000 μ/mL penicillin and 10000 μg/mL streptomycin) is added into each well, followed by incubation at 37° C. and 4-8% CO.sub.2. The culture medium is replaced every other day. The cells are collected when the cells are grown to form clones and further form colonies wherein 80% or more of the clones contain 40-100 cells and clones of grade A and grade B are observed in three fields of view among randomly selected five fields of view.

[0074] Generally cell clones appear after 4-7 days. The cell clones can be classified as grades A, B, and C depending on the morphology. The clones of grade A have a regular and smooth outline, and the clones show clear boundary, and cells in the clones are closely packed and have uniform size. The clones of grade B have a basically regular outline, and the clones show smooth and clear boundary, and in the clones most of the cells are closely packed and have uniform size but a small amount of the cells have a slightly larger size and are slightly loose packed. The clones of grade C have an irregular outline and unclear boundary, and the cells in the clones are loosely packed and have large size.

[0075] If there are very few cell clones, five fields of view are observed under a 10-fold microscope, and if there are no clones of grade A or B in three of the five fields of view, the cells in the well need to be detached via digestion and plated onto the feeder layer cells previously prepared the day before at a ratio of 1:1 to perform further culture.

[0076] The collected cells are cultured in a 6 cm culture dish pre-plated with feeder layer cells. When the cells are grown to cover 50%-90% of the culture dish surface, first passage is carried out as follows: Culture supernatant is removed, and the cells are washed once with 1×DPBS, then digested with 1 mL of 0.25% (mass/volume ratio: 0.25 g/100 mL) trypsin at 37° C. for 5-10 minutes. Adherent cells are detached from the plate by pipetting up and down and prepared into a single-cell suspension when most of the cells become round and bright. The digestion is terminated with 1 mL of stop buffer, and the resulting cell suspension is collected and centrifuged at 1200 rpm for 3 minute. Supernatant is removed, and the cells are re-suspended in the culture medium. Finally the resulting suspension is plated onto one 6 cm culture dish at a density of 5-10×10.sup.5 cells. The culture medium is replaced every other day.

[0077] 2nd passage is carried out when the first passage grows to cover 50%-90% of the culture dish surface. The operation is as follows: Culture supernatant is removed, and the cells are washed once with 1×DPBS, then digested with 1 mL of 0.25% (mass/volume ratio: 0.25 g/100 mL) trypsin at 37° C. for 5-10 minutes. Adherent cells are detached by pipetting up and down and prepared into a single-cell suspension when most of the cells become round and bright. The digestion is terminated with 1 mL of stop buffer, and the resulting cell suspension is collected and centrifuged at 1200 rpm for 3 minutes. Supernatant is removed, and the cells are re-suspended in culture medium. 1.1 mL supernatant and 3-16×10.sup.5 cells are collected to carry out tests, wherein bacterial detection and mycoplasma detection give negative results, and biological effect test gives a HOPX positive cell percentage of ≥30%, and cell identification test confirms cells are KRT5 positive, and purity analysis shows KRT5 cell percentage is ≥90%. Meanwhile the re-suspended cells are plated into four 10 cm culture dishes pre-coated with feeder layer cells at 5-20×10.sup.5 cells per dish, and culture medium is replaced every other day. 0.8 mL culture supernatant of passage 2 is collected to carry out endotoxin content detection and streptomycin residue detection, and the results show that endotoxin content <0.125 Eu/mL, and streptomycin residue <4 ppb.

[0078] 3rd passage is carried out when passage 2 grows to cover 50%-90% of the culture dish surface. The operation is as follows: Supernatant is removed, and the cells are washed once with 1×DPBS, digested with 2 mL of 0.05% (mass/volume ratio: 0.05 g/100 mL) trypsin at 37° C. for 1-2 minutes. The feeder layer cells are detached from the plate by pipetting up and down, again washed with 1×DPBS, and digested with 2 mL of 0.25% (mass/volume ratio: 0.05 g/100 mL) trypsin. Adherent cells are detached from the plate by pipetting up and down and prepared into a single-cell suspension when most of the cells become round and bright. The digestion is terminated with a stop buffer. A cell suspension is collected and centrifuged at 1200 rpm for 5 minutes, supernatant is removed, and the cells are re-suspended in culture medium. The resulting suspension is plated into twelve 10 cm culture dishes pre-coated with matrigel, and culture medium is replaced every other day.

[0079] Wherein, the culture dishes pre-coated with matrigel are prepared as follows: 20% matrigel is firstly added to coat the culture plate and overspread the bottom surface of the culture plate, and excess liquid is sucked out. Then the culture plate is placed at 37° C. for 15 minutes.

[0080] When the 3rd passage grows to cover 85%-95% of the culture dish surface, each culture dish is washed once with 1×DPBS. 2 mL of 0.25% (mass/volume ratio: 0.05 g/100 mL) trypsin is added, and the dish is placed at 37° C. for 5-15 min. Adherent cells are gently detached from the dish by pipetting up and down with a 1 mL pipette and prepared into a single-cell suspension when most of the cells become round and bright. The digestion is terminated with 2 mL of stop buffer, and a cell suspension is collected. Then the 10 cm culture dish is washed with 2 mL of stop buffer and all cell suspensions are collected. Since there is a large amount of cells to be processed in the present step, no more than five culture dishes are operated at the same time, and the collected cell suspensions are temporarily stored at 2-8° C. before all the dishes are processed. After all the cell suspensions of this batch are collected, the suspension is centrifuged at 1200 rpm for 5 min, supernatant is removed, and the cells are washed 2-4 times with 1×DPBS. The washing operation is as follows: re-suspended the centrifuged cell pellet with 40 mL of 1×DPBS, and centrifuged at 1200 rpm for 5 min, and supernatant is removed.

Example 2

[0081] Provided is a preparation process of clinical-grade autologous bronchial basal cells, comprising the following steps:

[0082] An ex vivo bronchoscopic brushed-off biopsy tissue is selected. Particularly tissues from two different sites of bronchus are selected in this example. Selecting tissues from two or more different sites of bronchus can increase the success rate of separation.

[0083] Tissue digestion solution and stop buffer are prepared for later use. The tissue digestion solution comprises 99 v % of DMEM/F12, and remaining is: 1-20 ng/mL of DNase, 0.1-4 mg/mL of protease Type XIV, and 10-200 ng/mL of trypsin. The stop buffer comprises 90 v % of DMEM and 10 v % FBS.

[0084] The bronchoscopic brushed-off biopsy tissue is digested with the tissue digestion solution. The digestion is terminated with a stop buffer, and digested cells are collected. Bacterial detection and mycoplasma detection are carried out for the digested cells, and then part of the digested cells are put into frozen storage.

[0085] The digested cells in frozen storage are recovered. The operation is as follows: The digested cells in frozen storage are rapidly thawed in a 37° C. water bath, and all of the resulting liquid is collected in a 15 mL tube, into which 2 mL of stop buffer previously warmed at 37° C. is added dropwise. The tube is centrifuged at 1200 rpm for 3 min, supernatant is removed, and the cells are re-suspended in culture medium to prepare a suspension.

[0086] A culture medium is prepared for later use, wherein the culture medium comprises 225 mL of DMEM, 225 mL of F12, 20-70 mL of FBS, 0.2-2 mM L-glutamine, 1-14 ng/mL of insulin, 0.1-1 ng/mL epidermal growth factor, 5-30 μg/mL adenine, and 2-20 μg/mL hydrocortisone.

[0087] A culture plate pre-coated with feeder layer cells is prepared for later use. The preparation of the plate is as follows: 20% matrigel is firstly added to coat the culture plate and overspread the bottom surface of the culture plate, and excess liquid is sucked out. Then the culture plate is placed at 37° C. for 15 minutes, thereafter irradiation-inactivated feeder layer cells are plated and cultured in a cell incubator (37° C., 5% CO.sub.2). After 1-2 days, the plate is ready for use if the cells spread 50-70% of the bottom surface of the culture plate.

[0088] A quarter of the previously suspension of the recovered digested cells is re-suspended in the culture medium and plated into one well of a 6-well culture plate pre-plated with the feeder layer cells, and the remaining three quarters are re-suspended in culture medium and plated into another well of the 6-well culture plate pre-plated with the feeder layer cells. 200 uL of penicillin-streptomycin solution (10000 μ/mL penicillin and 10000 μg/mL streptomycin) is added into each well, followed by incubation at 37° C. and 4-8% CO.sub.2. The culture medium is replaced every other day. The cells are collected when the cells are grown to form clones and further form colonies wherein 80% or more of the clones contain 40-100 cells and clones of grade A and grade B are observed in three fields of view among randomly selected five fields of view.

[0089] Generally cell clones appear after 4-7 days. The cell clones can be classified as grades A, B, and C depending on the morphology. The clones of grade A have a regular and smooth outline, and the clones show clear boundary, and cells in the clones are closely packed and have uniform size. The clones of grade B have a basically regular outline, and the clones show smooth and clear boundary, and in the clones most of the cells are closely packed and have uniform size but a small amount of the cells have a slightly larger size and are slightly loose packed. The clones of grade C have an irregular outline and unclear boundary, and the cells in the clones are loosely packed and have large size.

[0090] If there are very few cell clones, five fields of view are observed under a 10-fold microscope, and if there are no clones of grade A or B in three of the five fields of view, the cells in the well need to be detached via digestion and plated onto the feeder layer cells previously prepared the day before at a ratio of 1:1 to perform further culture.

[0091] 1.1 mL of the culture supernatant is collected to perform bacterial detection and mycoplasma detection before the cells are put into frozen storage.

[0092] When the cells are grown to cover 50%-90% of the culture dish surface, first passage is carried out as follows: Culture supernatant is removed, and the cells are washed once with 1×DPBS, then digested with 1 mL of 0.25% (mass/volume ratio: 0.25 g/100 mL) trypsin at 37° C. for 5-10 minutes. Adherent cells are detached from the plate by pipetting up and down and prepared into a single-cell suspension when most of the cells become round and bright. The digestion is terminated with 1 mL of stop buffer, and the resulting cell suspension is collected and centrifuged at 1200 rpm for 3 minute. Supernatant is removed, and the cells are re-suspended in the culture medium. Finally the resulting suspension is plated onto one 6 cm culture dish at a density of 5-10×10.sup.5 cells. The culture medium is replaced every other day.

[0093] 2nd passage is carried out when the first passage grows to cover 50%-90% of the culture dish surface. The operation is as follows: Culture supernatant is removed, and the cells are washed once with 1×DPBS, then digested with 1 mL of 0.25% (mass/volume ratio: 0.25 g/100 mL) trypsin at 37° C. for 5-10 minutes. Adherent cells are detached from the plate by pipetting up and down and prepared into a single-cell suspension when most of the cells become round and bright. The digestion is terminated with 1 mL of stop buffer, and the resulting cell suspension is collected and centrifuged at 1200 rpm for 3 minutes. Supernatant is removed, and the cells are re-suspended in culture medium. 1.1 mL supernatant and 3-16×10.sup.5 cells are collected to carry out tests, wherein bacterial detection and mycoplasma detection give negative results, and biological effect test gives a HOPX positive cell percentage of ≥30%, and cell identification test confirms cells are KRT5 positive, and purity analysis shows KRT5 cell percentage is ≥90%. Meanwhile the re-suspended cells are plated into four 10 cm culture dishes pre-coated with feeder layer cells at 5-20×10.sup.5 cells per dish, and culture medium is replaced every other day. 0.8 mL culture supernatant of passage 2 is collected to carry out endotoxin content detection and streptomycin residue detection, and the results show that endotoxin content <0.125 Eu/mL, and streptomycin residue <4 ppb.

[0094] 3rd passage is carried out when passage 2 grows to cover 50%-90% of the culture dish surface. The operation is as follows: Supernatant is removed, and the cells are washed once with 1×DPBS, digested at 37° C. for 1-2 minutes with 2 mL of 0.05% (mass/volume ratio: 0.05 g/100 mL) trypsin supplemented with 1.6mL of 1×DPBS. The feeder layer cells are detached from the plate by pipetting up and down, again washed with 1×DPBS, and digested with 2 mL of 0.25% (mass/volume ratio: 0.05 g/100 mL) trypsin. Adherent cells are detached from the plate by pipetting up and down and prepared into a single-cell suspension when most of the cells become round and bright. The digestion is terminated with a stop buffer. A cell suspension is collected and centrifuged at 1200 rpm for 5 minutes, supernatant is removed, and the cells are re-suspended in culture medium. The resulting suspension is plated into twelve 10 cm culture dishes pre-coated with matrigel, and culture medium is replaced every other day.

[0095] Wherein, the culture dishes pre-coated with matrigel are prepared as follows: 20% matrigel is firstly added to coat the culture plate and overspread the bottom surface of the culture plate, and excess liquid is sucked out. Then the culture plate is placed at 37° C. for 15 minutes.

[0096] When the 3rd passage grows to cover 85%-95% of the culture dish surface, each culture dish is washed once with 1×DPBS. 2 mL of 0.25% (mass/volume ratio: 0.05 g/100 mL) trypsin is added, and the dish is placed at 37° C. for 5-15 min Adherent cells are gently detached from the dish by pipetting up and down with a 1 mL pipette and prepared into a single-cell suspension when most of the cells become round and bright. The digestion is terminated with 2 mL of stop buffer, and a cell suspension is collected. Then the 10 cm culture dish is washed with 2 mL of stop buffer and all cell suspensions are collected. Since there is a large amount of cells to be processed in the present step, no more than five culture dishes are operated at the same time, and the collected cell suspensions are temporarily stored at 2-8° C. before all the dishes are processed. After all the cell suspensions of this batch are collected, the suspension is centrifuged at 1200 rpm for 5 min, supernatant is removed, and the cells are washed 2-4 times with 1×DPBS. The washing operation is as follows: re-suspended the cell pellet with 40 mL of 1×DPBS, and centrifuged at 1200 rpm for 5 min, and supernatant is removed.

Example 3

[0097] Provided is a preparation process of clinical-grade autologous bronchial basal cells, comprising the following steps:

[0098] An ex vivo bronchoscopic brushed-off biopsy tissue is selected. Particularly tissues from two different sites of bronchus are selected in this example. Selecting tissues from two or more different sites of bronchus can increase the success rate of separation.

[0099] Tissue digestion solution and stop buffer are prepared for later use. The tissue digestion solution comprises 99 v % of DMEM/F12, and remaining is: 1-20 ng/mL of DNase, 0.1-4 mg/mL of protease Type XIV, and 10-200 ng/mL of trypsin. The stop buffer comprises 90 v % of DMEM and 10 v % FBS.

[0100] The bronchoscopic brushed-off biopsy tissue is digested with the tissue digestion solution. The digestion is terminated with a stop buffer, and digested cells are collected. Bacterial detection and mycoplasma detection are carried out for the digested cells, and then part of the digested cells are put into frozen storage. A culture medium is prepared for later use, wherein the culture medium comprises 225 mL of DMEM, 225 mL of F12, 20-70 mL of FBS, 0.2-2 mM L-glutamine, 1-14 ng/mL of insulin, 0.1-1 ng/mL epidermal growth factor, 5-30 μg/mL adenine, and 2-20 μg/mL hydrocortisone.

[0101] A culture plate pre-coated with feeder layer cells is prepared for later use. The preparation of the plate is as follows: 20% matrigel is firstly added to coat the culture plate and overspread the bottom surface of the culture plate, and excess liquid is sucked out. Then the culture plate is placed at 37° C. for 15 minutes, thereafter irradiation-inactivated feeder layer cells are plated and cultured in a cell incubator (37° C., 5% CO.sub.2). After 1-2 days, the plate is ready for use if the cells spread 50-70% of the bottom surface of the culture plate.

[0102] A part of the digested cells are taken to perform the following steps: A quarter of the cells are re-suspended in the culture medium and plated into one well of a 6-well culture plate pre-plated with the feeder layer cells, and the remaining three quarters are re-suspended in culture medium and plated into another well of the 6-well culture plate pre-plated with the feeder layer cells. 200 μL of penicillin-streptomycin solution (10000 μ/mL penicillin and 10000 μg/mL streptomycin) is added into each well, followed by incubation at 37° C. and 4-8% CO.sub.2. The culture medium is replaced every other day. The cells are collected when the cells are grown to form clones and further form colonies wherein 80% or more of the clones contain 40-100 cells and clones of grade A and grade B are observed in three fields of view among randomly selected five fields of view.

[0103] Generally cell clones appear after 4-7 days. The cell clones can be classified as grades A, B, and C depending on the morphology. The clones of grade A have a regular and smooth outline, and the clones show clear boundary, and cells in the clones are closely packed and have uniform size. The clones of grade B have a basically regular outline, and the clones show smooth and clear boundary, and in the clones most of the cells are closely packed and have uniform size but a small amount of the cells have a slightly larger size and are slightly loose packed. The clones of grade C have an irregular outline and unclear boundary, and the cells in the clones are loosely packed and have large size.

[0104] If there are very few cell clones, five fields of view are observed under a 10-fold microscope, and if there are no clones of grade A or B in three of the five fields of view, the cells in the well need to be detached via digestion and plated onto the feeder layer cells previously prepared the day before at a ratio of 1:1 to perform further culture.

[0105] 1.1 mL of the culture supernatant is collected to perform bacterial detection and mycoplasma detection before the cells are put into frozen storage. Following steps are conducted if the detection results are satisfactory.

[0106] When there is a manufacturing order, the frozen cells are taken out, one tube for cells from each site. The frozen cells are rapidly thawed in a 37° C. water bath, and all of the resulting liquid is collected in a 15 mL tube, into which 2 mL of stop buffer previously warmed at 37° C. is added dropwise. The tube is centrifuged at 1200 rpm for 3 minutes, supernatant is removed, and the cells are re-suspended in culture medium and cultured in a 6-well culture plate pre-coated with feeder layer cells. During the culture, morphology of the cell clones is observed and monitored. The qualification standard is that: clones of grade A and clones of grade B exceed 50% in five randomly observed fields of view. The following steps are conducted when the quality standard is met. In said culture plate pre-coated with feeder layer cells, 50-70% of the culture plate area is pre-coated with feeder layer cells.

[0107] When the thawed cells are grown to cover 50%-90% of the culture dish surface, first passage is carried out as follows: Culture supernatant is removed, and the cells are washed once with 1×DPBS, then digested with 1 mL of 0.25% (mass/volume ratio: 0.25 g/100 mL) trypsin at 37° C. for 5-10 minutes. Adherent cells are detached from the plate by pipetting up and down and prepared into a single-cell suspension when most of the cells become round and bright. The digestion is terminated with 1 mL of stop buffer, and the resulting cell suspension is collected and centrifuged at 1200 rpm for 3 minute. Supernatant is removed, and the cells are re-suspended in the culture medium. Finally the resulting suspension is plated onto one 6 cm culture dish at a density of 5-10×10.sup.5 cells. The culture medium is replaced every other day.

[0108] 2nd passage is carried out when the first passage grows to cover 50%-90% of the culture dish surface. The operation is as follows: Culture supernatant is removed, and the cells are washed once with 1×DPBS, then digested with 1 mL of 0.25% (mass/volume ratio: 0.25 g/100 mL) trypsin at 37° C. for 5-10 minutes. Adherent cells are detached from the plate by pipetting up and down and prepared into a single-cell suspension when most of the cells become round and bright. The digestion is terminated with 1 mL of stop buffer, and the resulting cell suspension is collected and centrifuged at 1200 rpm for 3 minutes. Supernatant is removed, and the cells are re-suspended in culture medium. 1.1 mL supernatant and 3-16×10.sup.5 cells are collected to carry out tests, wherein bacterial detection and mycoplasma detection give negative results, and biological effect test gives a HOPX positive cell percentage of ≥30%, and cell identification test confirms cells are KRT5 positive, and purity analysis shows KRT5 cell percentage is ≥90%. Meanwhile the re-suspended cells are plated into three 10 cm culture dishes pre-coated with feeder layer cells at 5-20×10.sup.5 cells per dish, and culture medium is replaced every other day. 0.8 mL culture supernatant of passage 2 is collected to carry out endotoxin content detection and streptomycin residue detection, and the results show that endotoxin content <0.125 Eu/mL, and streptomycin residue <4 ppb.

[0109] 3rd passage is carried out when passage 2 grows to cover 50%-90% of the culture dish surface. The operation is as follows: Supernatant is removed, and the cells are washed once with 1×DPBS, digested with 2 mL of 0.05% (mass/volume ratio: 0.05 g/100 mL) trypsin at 37° C. for 1-2 minutes. The feeder layer cells are detached from the plate by pipetting up and down, again washed with 1×DPBS, and digested with 2 mL of 0.25% (mass/volume ratio: 0.05 g/100 mL) trypsin. Adherent cells are detached from the plate by pipetting up and down and prepared into a single-cell suspension when most of the cells become round and bright. The digestion is terminated with a stop buffer. A cell suspension is collected and centrifuged at 1200 rpm for 5 minutes, supernatant is removed, and the cells are re-suspended in culture medium. The resulting suspension is all plated into twelve culture dishes pre-coated with matrigel, and culture medium is replaced every other day.

[0110] Wherein, the culture dishes pre-coated with matrigel are prepared as follows: 20% matrigel is firstly added to coat the culture plate and overspread the bottom surface of the culture plate, and excess liquid is sucked out. Then the culture plate is placed at 37° C. for 15 minutes.

[0111] When the 3rd passage grows to cover 85%-95% of the culture dish surface, each culture dish is washed once with 1×DPBS. 2 mL of 0.25% (mass/volume ratio: 0.05 g/100 mL) trypsin is added, and the dish is placed at 37° C. for 5-15 min Adherent cells are gently detached from the dish by pipetting up and down with a 1 mL pipette and prepared into a single-cell suspension when most of the cells become round and bright. The digestion is terminated with 2 mL of stop buffer, and a cell suspension is collected. Then the 10 cm culture dish is washed with 2 mL of stop buffer and all cell suspensions are collected. Since there is a large amount of cells to be processed in the present step, no more than five culture dishes are operated at the same time, and the collected cell suspensions are temporarily stored at 2-8° C. before all the dishes are processed. After all the cell suspensions of this batch are collected, the suspension is centrifuged at 1200 rpm for 5 min, supernatant is removed, and the cells are washed 2-4 times with 1×DPBS. The washing operation is as follows: re-suspended the cell pellet with 40 mL of 1×DPBS, and centrifuged at 1200 rpm for 5 min, and supernatant is removed.

Example 4

[0112] Provided is a preparation process of a deliverable formulation of clinical-grade autologous bronchial basal cells. As shown in FIG. 1, the preparation process is as follows:

[0113] An ex vivo bronchoscopic brushed-off biopsy tissue is selected. Particularly tissues from two different sites of bronchus are selected in this example. Selecting tissues from two or more different sites of bronchus can increase the success rate of separation.

[0114] Tissue digestion solution and stop buffer are prepared for later use. The tissue digestion solution comprises 99 v % of DMEM/F12, and remaining is: 1-20 ng/mL of DNase, 0.1-4 mg/mL of protease Type XIV, and 10-200 ng/mL of trypsin. The stop buffer comprises 90 v % of DMEM and 10 v % FBS.

[0115] The bronchoscopic brushed-off biopsy tissue is digested with the tissue digestion solution. The digestion is terminated with a stop buffer, and digested cells are collected. Bacterial detection and mycoplasma detection are carried out for the digested cells, and then part of the digested cells are put into frozen storage.

[0116] A culture medium is prepared for later use, wherein the culture medium comprises 225 mL of DMEM, 225 mL of F12, 20-70 mL of FBS, 0.2-2 mM L-glutamine, 1-14 ng/mL of insulin, 0.1-1 ng/mL epidermal growth factor, 5-30 μg/mL adenine, and 2-20 μg/mL hydrocortisone.

[0117] A culture plate pre-coated with feeder layer cells is prepared for later use. The preparation of the plate is as follows: 20% matrigel is firstly added to coat the culture plate and overspread the bottom surface of the culture plate, and excess liquid is sucked out. Then the culture plate is placed at 37° C. for 15 minutes, thereafter irradiation-inactivated feeder layer cells are plated and cultured in a cell incubator (37° C., 5% CO.sub.2). After 1-2 days, the plate is ready for use if the cells spread 50-70% of the bottom surface of the culture plate.

[0118] A part of the digested cells are taken to perform the following steps: A quarter of the cells are re-suspended in the culture medium and plated into a 6-well culture plate pre-plated with the feeder layer cells, and the remaining three quarters are re-suspended in culture medium and plated into another 6-well culture plate pre-plated with the feeder layer cells. 200 μL of penicillin-streptomycin solution (10000 μ/mL penicillin and 10000 μg/mL streptomycin) is added into each well, followed by incubation at 37° C. and 4-8% CO.sub.2. The culture medium is replaced every other day. When the cells are grown to form clones and further form colonies (as shown in FIG. 2) wherein 80% or more of the clones contain 40-100 cells and clones of grade A and grade B are observed in three fields of view among five randomly selected fields of view (morphologies of clones of grade A and grade B are respectively as shown in FIG. 3-A and FIG. 3-B, wherein larger sized cells are circled), the cells are collected and are put into frozen storage according to sampling sites of bronchoscopic brushed-off biopsy tissue.

[0119] Generally cell clones appear after 4-7 days. The cell clones can be classified as grades A, B, and C depending on the morphology. The clones of grade A have a regular and smooth outline, and the clones show clear boundary, and cells in the clones are closely packed and have uniform size. The clones of grade B have a basically regular outline, and the clones show smooth and clear boundary, and in the clones most of the cells are closely packed and have uniform size but a small amount of the cells have a slightly larger size and are slightly loose packed. The clones of grade C have an irregular outline and unclear boundary, and the cells in the clones are loosely packed and have large size.

[0120] If there are very few cell clones, five fields of view are observed under a 10-fold microscope, and if there are no clones of grade A or B in three of the five fields of view, the cells in the well need to be detached via digestion and plated onto the feeder layer cells previously prepared the day before at a ratio of 1:1 to perform further culture. The cells are collected and put into frozen storage when the cells are grown to form clones and further form colonies wherein 80% or more of the clones are grown to contain 40-100 cells. The culture supernatant is collected to perform bacterial detection and mycoplasma detection (In-process control, point 1) before the cells are put into frozen storage. The following steps are performed if the detection results are qualified.

[0121] When there is a manufacturing order, the frozen cells are taken out, one tube for cells from each sampling site. The frozen cells are rapidly thawed in a 37° C. water bath, and all of the resulting liquid is collected in a 15 mL tube, into which 2 mL of stop buffer previously warmed at 37° C. is added dropwise. The tube is centrifuged at 1200 rpm for 3 minutes, supernatant is removed, and the cells are re-suspended in culture medium and cultured on feeder layer cells prepared the day before (in one well of 6 wells). Observe every day and replace the culture medium every other day. Feeder layer cells coats 50-70% of the culture plate area. During the culture, morphology of the cell clones is observed and monitored. The qualification standard is that: clones of grade A and clones of grade B exceed 50% in five randomly observed fields of view. The following steps are conducted when the qualification standard is met.

[0122] When the thawed cells are grown to cover 50%-90% of the culture dish surface, first passage is carried out as follows: Culture supernatant is removed, and the cells are washed once with 1×DPBS, then digested with 1 mL of 0.25% (mass/volume ratio: 0.25 g/100 mL) trypsin at 37° C. for 5-10 minutes. Adherent cells are detached from the plate by pipetting up and down and prepared into a single-cell suspension when most of the cells become round and bright. The digestion is terminated with 1 mL of stop buffer, and the resulting cell suspension is collected and centrifuged at 1200 rpm for 3 minute. Supernatant is removed, and the cells are re-suspended in the culture medium. Finally the resulting suspension is plated onto one 6 cm culture dish at a density of 5-10×10.sup.5 cells. The culture medium is replaced every other day.

[0123] 2nd passage is carried out when the first passage grows to cover 50%-90% of the culture dish surface. The operation is as follows: Culture supernatant is removed, and the cells are washed once with 1×DPBS, then digested with 1 mL of 0.25% (mass/volume ratio: 0.25 g/100 mL) trypsin at 37° C. for 5-10 minutes. Adherent cells are detached from the plate by pipetting up and down and prepared into a single-cell suspension when most of the cells become round and bright. The digestion is terminated with 1 mL of stop buffer, and the resulting cell suspension is collected and centrifuged at 1200 rpm for 3 minutes. Supernatant is removed, and the cells are re-suspended in culture medium. 1.1 mL supernatant and 3-16×10.sup.5 cells are collected to carry out tests, wherein bacterial detection and mycoplasma detection give negative results, and biological effect test gives a HOPX positive cell percentage of and cells identification test confirms cells are KRT5 positive, and purity analysis shows KRT5 cell percentage is ≥90% (In-process control, point 2). Meanwhile the re-suspended cells are plated into three 10 cm culture dishes pre-coated with feeder layer cells at 5-20×10.sup.5 cells per dish, and culture medium is replaced every other day. 0.8 mL culture supernatant of passage 2 is collected to carry out endotoxin content detection and streptomycin residue detection, and the results show that endotoxin content <0.125 Eu/mL, and streptomycin residue <4 ppb (In-process control, point 3).

[0124] 3rd passage is carried out when passage 2 grows to cover 50%-90% of the culture dish surface. The operation is as follows: Supernatant is removed, and the cells are washed once with 1×DPBS, digested with 2 mL of 0.05% (mass/volume ratio: 0.05 g/100 mL) trypsin at 37° C. for 1-2 minutes. The feeder layer cells are detached from the plate by pipetting up and down, again washed with 1×DPBS, and digested with 2 mL of 0.25% (mass/volume ratio: 0.05 g/100 mL) trypsin. Adherent cells are detached from the plate by pipetting up and down and prepared into a single-cell suspension when most of the cells become round and bright. The digestion is terminated with a stop buffer. A cell suspension is collected and centrifuged at 1200 rpm for 5 minutes, supernatant is removed, and the cells are re-suspended in culture medium. The resulting suspension is all plated into twelve 10 cm culture dishes pre-coated with matrigel, and culture medium is replaced every other day.

[0125] Wherein, the culture dishes pre-coated with matrigel are prepared as follows: 20% matrigel is firstly added to coat the culture plate and overspread the bottom surface of the culture plate, and excess liquid is sucked out. Then the culture plate is placed at 37° C. for 15 minutes.

[0126] When the 3rd passage grows to cover 85%-95% of the culture dish surface, each culture dish is washed once with 1×DPBS. 2 mL of 0.25% (mass/volume ratio: 0.05 g/100 mL) trypsin is added, and the dish is placed at 37° C. for 5-15 min Adherent cells are gently detached from the dish by pipetting up and down with a 1 mL pipette and prepared into a single-cell suspension when most of the cells become round and bright. The digestion is terminated with 2 mL of stop buffer, and a cell suspension is collected. Then the 10 cm culture dish is washed with 2 mL of stop buffer and all cell suspensions are collected. Since there is a large amount of cells to be processed in the present step, five culture dishes are operated at the same time, and the collected cell suspensions are temporarily stored at 2-8° C. before all the dishes are processed. After all the cell suspensions of this batch are collected, the suspension is centrifuged at 1200 rpm for 5 min, supernatant is removed.

[0127] The cell pellet are re-suspended in 45 mL 1×DPBS. All of the resulting cell suspension is collected into a 50 mL centrifuge tube, and centrifuged at 1200 rpm for 5 minutes. Supernatant is removed. The cell pellet are re-suspended in 45 mL of 1×DPBS, mixed well by inverting the centrifuge tube upside down. 10 uL of the resulting cell suspension is collected for trypan blue staining, showing ≥90% cells are alive (In-process control, point 4). The cells in the centrifuge tube are centrifuged at 1200 rpm for 5 minutes, and supernatant is discarded. The cell pellets are re-suspended in 15 mL of normal saline to reach a cell concentration of 1-22×10.sup.6/mL. 1 mL of the resulting cell preparation is collected as a reserved sample. The appearance test shows that the deliverable formulation is a colorless or white cell suspension without obvious visible impurities (Final quality control, point 5).

[0128] The above mentioned bacterial detection is carried out with a plate culture method. Mycoplasma detection is carried out with PCR method.

[0129] Endotoxin detection is carried out with Limulus Amoebocyte Lysate (LAL) Gel-Clot method.

[0130] Streptomycin residue detection is carried out with competitive enzyme-linked immunosorbent assay (ELISA).

[0131] Biological effect test is carried out with in vitro differentiation culture method, and the cells are immuno-stained with a HopX antibody after differentiation.

[0132] Cell identification and cell purity detection are carried out with immunofluorescence staining method.

[0133] Detection of proportions of alive cells is carried out with Trypan blue staining method.

Experiment Example 1

[0134] A deliverable formulation of clinical-grade autologous bronchial basal cells for lung tissue regeneration medicine is stock-out and transported within 12 h by means of 2-8° C. of cold chain transportation into a hospital for treatment, and then delivered back to a subject through a bronchoscope. The specific steps are as follows:

[0135] (1) When the subject has a transcutaneous oxygen saturation of 92% or more and the vital signs are stable, the deliverable formulation of clinical-grade autologous bronchial basal cells is injected into the lobar or segmental bronchi of the lesion site through a bronchofibroscope.

[0136] (2) After the deliverable formulation of the clinical-grade autologous bronchial basal cells is delivered, the drug delivery tube is firstly taken out, and then the bronchofibroscope is taken out. If necessary, non-invasive continuous positive airway pressure (CPAP) therapy can be applied in order to assist bronchial basal cell attachment to the lung tissue. Fasting for solid food and liquids are required within 2 hours postoperation. The subject shall avoid coughing as much as possible, and codeine can be administered orally if necessary.

[0137] (3) After the delivery is completed, the subject is required to stay in a supine position for about two hours to ensure tight adhesion between the delivered cells and lung injury areas.

Experimental Example 2

[0138] YE, female, 43 years old, suffering from bronchiectasis and emphysema, accompanied by suspected old pulmonary tuberculosis. The treatment included two stages. First stage was to prepare a deliverable formulation of clinical-grade autologous bronchial basal cells, and second stage was to deliver the deliverable formulation of clinical-grade autologous bronchial basal cells back to the lung.

[0139] (1) Preparation of a deliverable formulation of clinical-grade autologous bronchial basal cells: YE received bronchoscope examination in September 2016 and trace amounts of brushed-off biopsy tissue were obtained by bronchoscopic brush biopsy. The formulation of clinical-grade autologous bronchial basal cells was prepared according to the preparation process as described in Example 4.

[0140] (2) Delivery of the deliverable formulation of clinical-grade autologous bronchial basal cells back to the lung

[0141] In April of 2016, the patient received treatment of the above prepared deliverable formulation of clinical-grade autologous bronchial basal cells according to the procedures as described in Experimental Example 1 in the Southwest Hospital of AMU, China.

[0142] From date of cell delivery to August of 2018, the subject did not exhibit adverse reactions associated with the formulation. Pulmonary function tests showed that, one month after cell delivery, the forced vital capacity/predicted (FVC %), which is an index of lung ventilation capacity, was increased to 58.0% from 49.5% at baseline. Three months later the FVC % was further increased to 63.6% and maintained for one year post-treatment. The diffusion capacity of carbon monoxide/predicted (DLCO %) was also increased from 56.3% before treatment to 68.2% one year post-treatment. The result of 6-minute walk test (6 MWT) of the patient was increased from 447 meters before treatment to 495 meters post-treatment (Table 1). The patient reported that she could climb up to the 5.sup.th floor after the treatment, while she could only climb up to the 1.sup.st or 2.sup.nd floor before the treatment. Symptoms, such as shortness of breath, wheeziness and coughing, were also greatly ameliorated. More importantly, comparison of HR-CT results of the subject before and after cell delivery showed that, new lung structures were obviously generated at the destroyed lesions of the right lung as the time goes after the treatment, so the structural damage in the patient's lung was repaired (FIG. 4). This is consistent with previously described improvements in the patient's pulmonary function test results, as well as obvious relief of the various symptoms, confirming the treatment with the deliverable formulation of clinical-grade autologous bronchial basal cells is effective.

TABLE-US-00001 TABLE 1 Results of pulmonary function tests and 6MWT of the patient before and after treatment 1 month 3 months 6 months 1 year Detection Before after after after after index treatment treatment treatment treatment treatment VC MAX % 54.7 57.1 63.9 63.9 63.9 FEV1% 41.1 34.4 41.8 38.9 45.8 FVC % 49.5 58.0 63.6 63.5 65 TLCO-SB % 56.3 57.1 61.8 67.3 68.2 6MWT (meter) 447 495 540 423 495

Experimental Example 3

[0143] In 2016, a subject with chronic obstructive pulmonary disease (chronic bronchitis) received treatment with the deliverable formulation of clinical-grade autologous bronchial basal cells in the Southwest Hospital of AMU, China. The treatment was divided into two stages, wherein the first stage was to prepare a deliverable formulation of clinical-grade autologous bronchial basal cells, and the second stage was to deliver the deliverable formulation of clinical-grade autologous bronchial basal cells back to the subject's lung.

[0144] (1) A deliverable formulation of clinical-grade autologous bronchial basal cells was prepared according to the preparation process as described in Example 4.

[0145] (2) The deliverable formulation of clinical-grade autologous bronchial basal cells was delivered back to the patient.

[0146] The patient received treatment of the above prepared deliverable formulation of clinical-grade autologous bronchial basal cells according to the procedures as described in Experimental Example 1. Half year after the treatment, the subject exhibited no adverse reactions associated with the formulation, while the subject's pulmonary function indexes, such as FEV1, FEV1/FVC, DLCO, MMEF, MVV, etc. were significantly increased, and the symptoms of the subject evaluated by the St George' Respiratory Questionnaire (SGRQ) were significantly improved (Table 2).

TABLE-US-00002 TABLE 2 Results of the pulmonary function tests, 6MWT, and SGRQ score of the subject before and after treatment Detection Before 1 month after 3 months after Half year after index treatment treatment treatment treatment FEV1% 31.7 27.8 37.8 41.0 FVC % 53.7 54.8 62.0 67.7 MMEF75/25% 10.9 9.4 13.0 14.1 TLCO-SB % 37.6 93.9 74.8 82.0 6MWT(meter) 522 429 570 510 SGRQ score 27 30 22 17

Experimental Example 4

[0147] LIANG, male, suffering from interstitial lung disease and CT images showing obvious pulmonary bullae in the right lower lung, received treatment with deliverable formulation of clinical-grade autologous bronchial basal cells in November 2016 in Shanghai East Hospital, China. The treatment was divided into two stages, wherein the first stage was to prepare a deliverable formulation of clinical-grade autologous bronchial basal cells, and the second stage was to deliver the deliverable formulation of clinical-grade autologous bronchial basal cells back to the patient's lung.

[0148] (1) A deliverable formulation of clinical-grade autologous bronchial basal cells was prepared according to the preparation process as described in Example 4.

[0149] (2) The deliverable formulation of clinical-grade autologous bronchial basal cells was delivered back to the patient.

[0150] The patient received treatment of the above prepared deliverable formulation of clinical-grade autologous bronchial basal cells according to the procedures as described in Experimental Example 1. CT images at one-month and three-month after the treatment showed significant remission of the pulmonary bullae in the right lower lung (FIG. 5). The subject reported evidently relieved symptoms. 6 months after the treatment, all pulmonary function indexes were increased significantly. Acute exacerbation rates were reduced from average 4-5 times per year before the treatment to almost none. CT results at 6 months after the cell delivery showed that, new lung structure was formed in the destroyed area of the left lung, and the original damage of the lung structure was mostly repaired (FIG. 6). This is consistent with the improvements in the results of the patient's other indexes and significant alleviation of the symptoms reported by the patient, confirming the treatment of the deliverable formulation of clinical-grade autologous bronchial basal cells is effective.

[0151] Apparently, the aforementioned embodiments are merely examples illustrated for clearly describing the present invention, rather than limiting the implementation ways thereof. For those skilled in the art, various changes and modifications in other different forms can be made on the basis of the aforementioned description. It is unnecessary and impossible to exhaustively list all the implementation ways herein. However, any obvious changes or modifications derived from the aforementioned description are intended to be embraced within the protection scope of the present invention.