BLEND FORMULATION COMPRISING SILICATE AND MICROBIAL AND / OR PLANT CELLS COMPRISING A POLYUNSATURATED FATTY ACID HAVING AT LEAST 20 CARBON ATOMS (LC-PUFA)

Abstract

The present invention relates to a blend formulation (I) comprising (i) at least 50 weight-% (wt-%), based on the total weight of the blend formulation, of a LC-PUFA-containing composition (ii) 10-50 wt-%, based on the total weight of silcate.

Claims

1. A powderous blend formulation (I) comprising: (i) microbial or plant cells, (ii) at least 50 weight percent (wt. %), based on the total weight of the blend formulation, of a composition comprising a long chain polyunsaturated fatty acid (LC-PUFA-containing composition); and (iii) 10-50 wt. %, based on the total weight of the blend formulation, of a silicate.

2. The powderous blend formulation according to claim 1, further comprising 0 to 10 wt. %, based on the total weight of the blend formulation, of water and/or an additive.

3. The powderous blend formulation according to claim 1, wherein the silicate consists of sepiolite.

4. The powderous blend formulation according to claim 3, wherein the sepiolite has an average particle size D(v, 0.5) in a range of 100 to 1500 μm.

5. The powderous blend formulation according to claim 1, wherein powder particles of the powderous blend formulation comprise a coating.

6. The powderous blend formulation according to claim 5, wherein the coating is selected from the group consisting of glycerine monostearate, carnauba wax, candelilla wax, sugarcane wax, palmitic acid, stearic acid hydrogenated cottonseed oil, hydrogenated palm oil, hydrogenated rapeseed oil and mixtures thereof.

7. The powderous blend formulation according to claim 1, wherein the LC-PUFA-containing composition has a moisture content of between 1 and 20 wt. %.

8. The powderous blend formulation according to claim 7, wherein the LC-PUFA-containing composition has an oil content of at least 10 wt. %.

9. The powderous blend formulation according to claim 8, wherein the LC-PUFA-containing composition comprises an oil which comprises at least 10 wt. % to below 80 wt. % of polyunsaturated fatty acids (PUFAs) with at least 3 double bonds with respect to total fatty acids in the oil.

10. The powderous blend formulation according to claim 7, wherein the long chain polyunsaturated acids (LC-PUFAs) in the LC-PUFA-containing composition have at least 3 double bonds.

11. The powderous blend formulation according to claim 1, wherein the cells comprise a microorganism of the order Thraustochytriales.

12. The powderous blend formulation according to claim 1, wherein the cells comprise a microorganism of the genus Thraustochytrium or Schizochytrium.

13. The powderous blend formulation according to claim 1, wherein the cells comprise docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA).

14. Feed products comprising the powderous blend formulation according to claim 1.

Description

EXAMPLES

Example 1

[0072] Fermentation broth of Mortierella alpina, obtained after 8 days of fermentation was pasteurized at 70° C. for 1 hour. The pasteurized broth was filtered, resulting in a filter cake have a moisture content of 50 wt. %. The filter cake was crumbled and extruded at a temperature below 15° C. The extrudate (diameter 3 mm) was dried in a continuous fluid bed drier with three zones to a moisture content of 7%. In the first zone the bed temperature was 32° C. and the air temperature 50° C. (T.sub.dew point=15° C.). 1.sup.st zone: bed temperature 32° C., the air temperature 50° C. (T.sub.dew point=15° C.): 45 minutes 2.sup.nd zone: bed temperature 32° C., air temperature 35° C. (T.sub.dew point=1° C.): 45 minutes 3.sup.rd zone: bed temperature 15° C., air temperature 15° C. (T.sub.dew point=1° C.): 30 minutes The oil content of the dried biomass was 39%. The ARA content was 46% with respect to the total fatty acids in the oil.

[0073] For the preparation of the blend formulation according to the invention, the silica compound is blended with the dried biomass in a separate mixing step.

Example 2

[0074] Seeds containing 19% Arachidonic acid (with respect to total fatty acids) are obtained from transgenic Brassica plants that are transformed using the procedures described in WO2008009600.

[0075] The seeds have the following specifications (determined in accordance with the Official Grain Grading Guide, 2001 of the Canadian Grain Commission): distinctly green<2%, total damaged<5%.

[0076] The seeds, having a moisture content of 17 wt. %, are dried using a fluid bed drier. The bed temperature is 28° C. Conditioned air is used having a dew point of 10° C. The dried seeds have a moisture content of 8.5 wt. %. The oil content is 35 wt. %.

[0077] For the preparation of the blend formulation according to the invention, the silica compound is blended with the dried seed in a separate mixing step.

Example 3 Silicate Powder for Use According to the Invention

[0078]

TABLE-US-00001 Sepiolite: Particle size Sepiolite D1 D2 D3 Sepiolite type 1 170 μm  367 μm  412 μm Sepiolite type 2 713 μm 1038 μm 1486 μm Sepiolite type 3 424 μm  631 μm  950 μm