COMBINATION SEROTONIN SPECIFIC REUPTAKE INHIBITOR AND SEROTONIN 1A RECEPTOR PARTIAL AGONIST FOR REDUCING L-DOPA-INDUCED DYSKINESIA
20210393621 · 2021-12-23
Inventors
- Christopher Roy Bishop (Vestal, NY, US)
- Anthony West (North Chicago, IL, US)
- Fredric Manfresson (Grand Rapids, MI, US)
Cpc classification
A61P25/14
HUMAN NECESSITIES
A61K31/4545
HUMAN NECESSITIES
A61K31/5377
HUMAN NECESSITIES
A61K45/06
HUMAN NECESSITIES
A61K31/198
HUMAN NECESSITIES
A61K31/496
HUMAN NECESSITIES
A61K31/495
HUMAN NECESSITIES
A61K31/198
HUMAN NECESSITIES
A61K31/5377
HUMAN NECESSITIES
A61K9/0053
HUMAN NECESSITIES
A61P1/06
HUMAN NECESSITIES
A61K2300/00
HUMAN NECESSITIES
A61K31/496
HUMAN NECESSITIES
A61K2300/00
HUMAN NECESSITIES
International classification
A61K31/496
HUMAN NECESSITIES
A61K31/198
HUMAN NECESSITIES
A61K31/4545
HUMAN NECESSITIES
A61K45/06
HUMAN NECESSITIES
Abstract
A method of treating and attenuating L-DOPA-induced dyskinesia, comprising administering an effective dose of at least one pharmacological agent, e.g., vilazodone, having serotonin-specific reuptake inhibition (SSRI) and serotonin receptor 1A (5-HT1AR) partial agonism activity, in conjunction with L-DOPA. Other agents, such as an L-DOPA decarboxylase inhibitor, e.g., carbidopa, or other adjunct treatments may also be provided.
Claims
1. A method of treating or reducing risk of a dyskinesia in a human patient, comprising administering an agent having a serotonin-specific reuptake inhibitor activity and a 5-HT1A receptor agonist activity, in a sufficient amount, and for a sufficient duration, to treat the dyskinesia of the human patient.
2-3. (canceled)
4. The method according to claim 1, wherein further comprising administering L-DOPA to the patent, and the agent is administered according to a protocol effective to reduce L-DOPA induced dyskinesia (LID).
5. The method according to claim 4, wherein the agent comprises at least one of vilazodone or vortioxetine, in an amount of 5 mg or less, which are administered to the patient within a common pharmaceutically acceptable dosage form comprising an effective amount of the L-DOPA to treat a movement disorder in the patient.
6. The method according to claim 1, wherein the agent comprises vilazodone.
7. The method according to claim 1, wherein the agent comprises vortioxetine.
8. The method according to claim 1, wherein the agent comprises hypidone.
9. The method according to claim 4, further comprising administering a peripherally-acting DOPA decarboxylase inhibitor to the human patient.
10-14. (canceled)
15. The method according to claim 1, further comprising administering at least one of a Catechol-O-methyl transferase inhibitors monoamine oxidase type B inhibitor, a dopamine receptor agonist, an anticholinergic agent, an antimuscarinic agent, benzatropine, diphenylhydramine, dimenhydrinate, scopolamine, cannabidiol (CBD), and cannabidiolic acid (CBDA) to the human patient to the human patient.
16. The method according to claim 1, further comprising administering amantadine to the human patient.
17-53. (canceled)
54. A pharmaceutical oral unit dosage form, comprising L-DOPA and an agent having activity both as serotonin-selective reuptake inhibitor and as a 5-HT1A receptor partial agonist.
55. The pharmaceutical dosage form according to claim 54, wherein the agent is selected from the group consisting of vilazodone and vortioxetine, in an amount of 5 mg or less, and the L-DOPA is in an amount of between 100 mg and 250 mg.
56-59. (canceled)
60. The pharmaceutical oral unit dosage form according to claim 54, wherein the at least one agent comprises vilazodone.
61. The pharmaceutical oral unit dosage form according to claim 54, wherein the at least one agent comprises vortioxetine.
62. The pharmaceutical oral unit dosage form according to claim 54, wherein the at least one agent comprises hypidone.
63. The pharmaceutical dosage form according to claim 54, further comprising a peripherally-acting DOPA decarboxylase inhibitor.
64-68. (canceled)
69. The pharmaceutical oral unit dosage form according to claim 54, further comprising at least one of a Catechol-O-methyl transferase inhibitor, a monoamine oxidase type B inhibitor, a dopamine receptor agonist, an anticholinergic agent, an antimuscarinic agent, benzatropine, diphenylhydramine, dimenhydrinate, scopolamine, cannabidiol (CBD), and cannabidiolic acid (CBDA).
70. The pharmaceutical oral unit dosage form according to claim 54, further comprising amantadine.
71-94. (canceled)
95. The pharmaceutical oral unit dosage form according claim 54, comprising at least 100 mg L-DOPA, at least 10 mg of a peripherally-acting DOPA decarboxylase inhibitor, and between 2.5-40 mg vilazodone or vortioxetine.
96. The pharmaceutical oral unit dosage form according to claim 54, wherein the L-DOPA is formulated with an extended release pharmacokinetic profile.
97-98. (canceled)
99. A method of treating a human receiving L-DOPA for treatment of Parkinson's disease, and suffering from or at risk of L-DOPA-induced dyskinesia (LID), comprising administering to the human a sufficient amount of a pharmaceutically acceptable dosage form of a single compound which is both an SSRI and a 5-HT1AR partial agonist, selected from the group consisting of vilazodone, vortioxetine, and hypidone, to treat or reduce risk of LID, concurrent with administration of L-DOPA to the human.
100. (canceled)
Description
BRIEF DESCRIPTION OF THE DRAWINGS
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DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
Example 1
[0103] The serotonergic system is a well-established modulator of L-DOPA-induced dyskinesia (LID). To date, targeting serotonin (5-HT) transporters (SERT) or 5-HT1A receptors (5-HT1AR) has shown promise in reducing LID, however these strategies have yet to translate clinically. Ideally, a compound acting at both known anti-dyskinetic sites could optimize such 5-HT-mediated approaches. Vilazodone (VZD) is an FDA-approved antidepressant that acts as a selective serotonin reuptake inhibitor and a partial 5-HT1AR agonist, situating Vilazodone in a unique position to reduce LID, without compromising L-DOPA-mediated motor improvements.
[0104] In Experiments 1 and 2, L-DOPA-naïve and L-DOPA-primed animals were co-administered Vilazodone and 11) L-DOPA daily for 3 weeks to model sub-chronic use. In these experiments, Vilazodone significantly suppressed developing and established LID, without compromising the pro-motor effects of L-DOPA therapy. Post-mortem neurochemical analysis revealed that in the dopamine (DA)-depleted striatum, Vilazodone-L-DOPA co-treatment increased DA content, suggesting a normalization of DA kinetics in dyskinetic brain. Analysis of striatal gene expression revealed that Vilazodone treatment reduced L-DOPA-induced c-Fos and preprodynorphin mRNA overexpression, indicative of attenuated DA D1 receptor-mediated direct pathway over-activity.
[0105] In Experiment 3, when tested against 5-HT1AR and 5-HT1B receptor (5-HT1BR) antagonists, WAY100635 and NAS-181, respectively, WAY100635 alone partially attenuated Vilazodone's anti-dyskinetic efficacy, suggesting both SERT-dependent effects and 5-HT1AR in Vilazodone actions. Such findings implicate mechanisms of action for Vilazodone and its potential for repositioning against LID development and expression in PD.
[0106] L-3,4-dihidroxyphenylalanine (L-DOPA) remains the standard treatment for late-stage Parkinson's disease (PD) symptom management (Mercuri and Bernardi, 2005). Unfortunately, its chronic administration often results in abnormal involuntary movements (AIMs) termed L-DOPA-induced dyskinesia (LID; Garcia-Ruiz et al., 2011). LID pathogenesis is multifaceted, but unregulated dopamine (DA) release from raphe-striatal serotonin (5-HT) neurons is associated with LID in both PD patients and experimental models (de la Fuente-Fernandez et al., 2004; Navailles et al., 2010). This 5-HT gain-of-function is supported by evidence of increased striatal 5-HT innervation (Zeng et al., 2010), 5-HT transporter (SERT) expression (Rylander et al., 2010; Politis et al., 2014), and SERT: DA transporter (DAT) ratios (Conti et al., 2016; Roussakis et al., 2016), all which positively correlate with LID.
[0107] To treat LID, current serotonergic strategies have focused primarily on 5-HT1A receptors (5-HT1AR) or more recently SERT. 5-HT1AR agonists and selective 5-HT reuptake inhibitors (SSRIs) reduce LID in animal models (Bishop et al., 2012; Conti et al., 2014; Fidalgo et al., 2015; Huot et al., 2015). Despite their pre-clinical efficacy, 5-HT1AR agonists produce mild anti-dyskinetic efficacy and/or worsening motor symptoms in large clinical cohorts (Kannari et al., 2002; Goetz et al., 2007). Eltoprazine, a selective 5-HT1A/1B partial agonist, displays anti-dyskinetic efficacy in rodents, non-human primates, and patients though it too may attenuate L-DOPA's benefits (Bezard et al., 2013; Svenningsson et al., 2015). Clinical assessment of chronic SSRIs is limited to retrospective analyses reporting delays in LID onset, LID severity and reduced peak-dose LID (Mazzucchi et al., 2015).
[0108] Serotonergic compounds reduce LID through a diverse set of actions. For example, in the dorsal raphe nucleus (DRN), 5-HT1AR agonists temper raphe-striatal DA release via by stimulation of local 5-HT1AR autoreceptors (Eskow et al., 2009; Navailles et al., 2010), whereas in the striatum, they mitigate striatal over activity through local hetero-receptors (Bishop et al., 2009; Meadows et al., 2017; Munoz et al., 2008). In comparison, SSRIs appear to increase endogenous 5-HT at DRN 5-HT1AR autoreceptors to reduce LID while coincidentally blocking striatal DA uptake through SERT to maintain L-DOPA's anti-parkinsonian effects (Kannari et al., 2006; Navailles et al., 2010; Larsen et al., 2011; Conti et al., 2014). Therefore, combining the activity of SSRIs and 5-HT1AR agonists may enhance the anti-dyskinetic potential of both targets.
[0109] Vilazodone (VZD), an FDA-approved SSRI and 5-HT1AR partial agonist (Cruz, 2012; Owen, 2011) was during chronic treatment to suppress both LID development and expression hemi-parkinsonian rats, while maintaining L-DOPA efficacy. Neurochemical and cellular analyses revealed target specific modulation of monoamine neurotransmission and dyskinesia-related gene expression, suggesting engagement of unique mechanisms to optimize L-DOPA therapy.
[0110] Materials and methods: Studies used adult male Sprague-Dawley rats (N=85, 250 g upon arrival; Harlan Farms, N.Y., USA), housed in plastic cages (22×45×23 cm) with ad libitum access to standard laboratory chow (Rodent Diet 5001; Lab Diet Brentwood, Mo.) and water. The colony room was set on a 12 h light/dark cycle (lights on at 07:00 h) at 22-23° C. and animals were maintained in accordance with the Institutional Animal Care and Use Committee of Binghamton University and the ‘Guide for the Care and Use of Laboratory Animals’ (Institute of Laboratory Animal Resources, National Academic Press, 2011).
[0111] 6-Hydroxydopamine-lesion surgeries: One week after arrival, rats received unilateral vehicle or 6-hydroxydopamine (6-OHDA) injections to the left medial forebrain bundle (MFB) to destroy DA neurons. Rats were administered Desipramine HCl (25 mg/kg, i.p.; Sigma, St Louis, Mo.) and buprenorphine HCl (0.03 mg/kg, i.p.; Reckitt Benckiser Pharmaceuticals Inc., Richmond, Va.) 30 and 5 min prior to surgery to protect norepinephrine neurons and provide analgesia respectively. Rats were anesthetized with inhalant isoflurane (2-3%; Sigma) in oxygen (2.5 L/min), and placed in a stereotaxic apparatus (Kopf Instruments, Tujunga, Calif.). MFB injection coordinates were AP: −1.8 mm, ML: +2.0 mm, DV: −8.6 mm relative to bregma, with the incisor bar 5.0 mm below the interaural line (Paxinos and Watson, 1998). For infusions, a 26-gauge needle delivered 4 μL 6-OHDA (0 or 3 μg/μL; Sigma) dissolved in 0.9% NaCl+0.1% ascorbic acid at 2 μL/min. The needle was withdrawn 5 min post-infusion. Rats were provided with soft chow and saline to facilitate recovery and allowed 3 weeks before experimentation.
[0112] Experiment 1: Chronic intervention with Vilazodone in L-DOPA-primed, hemi-parkinsonian rats Rats (n=30) were rendered hemi-parkinsonian with unilateral 6-OHDA lesions of the left MFB. Three-weeks post-lesion, animals were divided into 2 equally disabled groups, measured by forepaw adjusting steps (FAS, see below). One group (n=8) began receiving 14 days of daily vehicle (0.9% NaCl+0.1% ascorbic acid) and the other (n=22) received L-DOPA methyl ester (6 mg/kg, s.c.; Sigma)+DL-serine 2-(2,3,4-trihydroxybenzyl) hydrazine hydrochloride (benserazide; 15 mg/kg, s.c.; Sigma), hereafter L-DOPA, dissolved in vehicle at a volume of 1 mg/kg. Treatment persisted for 14 days to produce stable AIMs expression (Putterman et al., 2007; Conti et al., 2014). Thereafter, L-DOPA-treated animals with Axial, Limb, and Orolingual (ALO) AIMs (see description below,
[0113] Experiment 2: Chronic Prevention with Vilazodone in L-DOPA-Naïve, Hemi-Parkinsonian Rats
[0114] Another cohort (n=38) received unilateral vehicle or 6-OHDA lesions. After recovery, FAS established baseline motor disability. 6-OHDA-lesioned animals were divided into 3 equally disabled groups. Rats then received daily injections of Vilazodone (0, 10, or 20 mg/kg, s.c.) 5 min prior to L-DOPA (0 or 6 mg/kg; s.c.) for 23 days in a between-subjects design. The 4 groups were: Sham+VZD (0)+LD (0), 6-OHDA Lesion+VZD (0)+LD (6), 6-OHDA Lesion+VZD (10)+LD (6), and 6-OHDA Lesion+VZD (20)+LD (6). AIMs (
[0115] Experiment 3: Characterizing the Role of the 5-HT1AR and 5-HT1BR in Vilazodone's Effects
[0116] All 6-OHDA-lesioned rats (n=17) were tested on the FAS prior to treatment regimens to establish baseline motor performance. Three-weeks post-surgery, all rats were primed with L-DOPA (6 mg/kg, s.c.) for 14 days. On days 1, 8, and 14 of L-DOPA treatment ALO AIMs were observed and rats with ALO AIMs scores <30 by day 14 were excluded in the study. Experiments began 3 days after cessation of priming and testing occurred every 3-4 days until completion.
[0117] One group of rats (n=9) received vehicle or the selective 5-HT1AR antagonist N-[2-[4(2-Methoxyphenyl)-1-piperazinyl] ethyl]-N-2-pyridinylcyclohexanecarboxamide maleate salt (WAY100635; 0.5 mg/kg, s.c.; Sigma) 5 min prior to vehicle or Vilazodone (10 mg/kg; s.c.) in a counterbalanced within-subjects design. All rats received injections of L-DOPA (6 mg/kg; s.c.) administered 5 min after their second injection after which AIMs rating commenced.
[0118] A second group of animals (n=8) received dH.sub.2O vehicle or the selective 5-HT1BR antagonist (R)-(+)-2-(3-morpholinomethyl-2H-chromen-8-yl) oxymethyl-morpholine methane-sulfonate (NAS-181; 3.0 mg/kg, s.c.; Fisher Scientific Hampton, N.H.) 5 min prior to vehicle or Vilazodone (10 mg/kg; s.c.) in a counterbalanced within-subjects design. All rats received injections of L-DOPA (6 mg/kg; s.c.) administered 5 min after their second injection after which AIMs were rated.
[0119] Behavioral Analyses
[0120] Abnormal involuntary movements (AIMs): The AIMs procedure measures rodent dyskinesia severity (Bishop et al., 2012). Beginning 10 min post-treatment, a trained and blinded observer assigned a severity score (0-4) to each of the ALO AIMs based on 1 min ratings every 10 min for 3 h: 0, not present; 1, present from 1-29 s; 2, present 30-59 s; 3, present all 60 s, but interruptible by a cylinder tap; 4, present 60 s and not interruptible by a cylinder tap.
[0121] Forepaw adjusting steps (FAS): The FAS test is a measure of forelimb akinesia performed according to previous protocols (Bishop et al., 2012; Chang et al., 1999). Data are presented as mean percent intact stepping where the sum of the total steps with the lesioned forepaw was divided by the total steps with the unlesioned forepaw multiplied by 100. Lower percent intact scores indicate greater forelimb akinesia. Prior to baseline, rats received 2 acclimation periods. On-treatment FAS was performed 60 min post-L-DOPA.
[0122] High-performance liquid chromatography (HPLC): HPLC, a method for semi-automated catecholamine analysis with coulometric detection, was performed according to prior protocols (Bishop et al., 2009) to determine the tissue levels of monoamines, their metabolites, and monoamine turnover in the striatum and DRN. The limit of detection for DOPAC, DA, 5-hydroxyindoleacetic acid (5-HIAA) and 5-HT was 10.sup.−10 M. Final oxidation current values were plotted on a standard curve from 10.sup.−6 M to 10.sup.−9 M and expressed as pg of monoamine or metabolite per mg of tissue unless otherwise specified.
[0123] Real-time RT-PCR: Striatal gene expression was measured by post-mortem analysis of c-Fos, preprodynorphin (PPD), preproenkephalin (PPE),) and 5-HT1AR mRNA expression normalized to the housekeeper gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Tissue was processed with RNeasy mini protocol (Qiagen, Hilden Germany), as detailed in prior work (Barnum et al., 2008). The following primer sequences were used:
TABLE-US-00001 GAPDH SEQ ID NO. 001 5′-GTGCCAGCCTCGTCTCATAG-3′/ SEQ ID NO. 002 5′-AGAGAAGGCAGCCCTGGTAA-3′ c-Fos SEQ ID NO. 003 5′-CCAAGCGGAGACAGATCAAC-3′/ SEQ ID NO. 004 5′-AAGTCCAGGGAGGTCACAGA-3′ PPD SEQ ID NO. 005 5′-GGGTTCGCTGGATTCAAATA-3′/ SEQ ID NO. 006 5′-TGTGTGGAGAGGGACACTCA-3′ PPE SEQ ID NO. 007 5′-AAAATCTGGGAGACCTGCAA-3′/ SEQ ID NO. 008 5′-CATGAAACCGCCATACCTCT-3′ 5-HT1AR SEQ ID NO. 009 5′-GATCTCGCTCACTTGGCTCA-3′/ SEQ ID NO. 010 5′′-AAAGCGCCGAAAGTGGAGTA-3′.
[0124] Statistical analyses: Parametric data are expressed as mean±standard error of the mean (SEM). FAS data were analyzed by 5×4 (Day×Treatment) mixed model ANOVAs. HPLC data were analyzed by 2×4 (Side'Treatment) and one-factor ANOVAs. RT-PCR data are reported as percent change from striata of vehicle-treated sham animals. Scores >2 S.D.s from the group mean were discarded and replaced with the new group mean (except for GAPDH). The data were analyzed using a 2×4 (Lesion×Treatment) mixed-model ANOVA. One subject was dropped from RT-PCR analysis because GAPDH was an outlier. Fisher's LSD post-hocs or planned comparisons between key conditions were employed as outlined below. Non-parametric AIMs data were expressed as medians±median absolute deviation (MAD). For within-subject designs, ALO AIMs were analyzed by Friedman ANOVAs and Wilcoxon post-hoc tests. ALO AIMs of between-subject designs were evaluated with Kruskal-Wallis ANOVAs and Mann-Whitney post-hocs. All statistical analyses were performed using SPSS 19.0 (IBM, Chicago, Ill.) with alpha set at 0.05.
[0125] Results
[0126] Experiment 1-Chronic Vilazodone reduces established LID: AIMs were monitored to determine Vilazodone-mediated changes to LID. During 14 days of priming, L-DOPA-treated groups did not significantly differ from each other, but each group produced significantly greater LID than animals administered L-DOPA vehicle (all χ.sup.2, (3)>17.481, p<0.02;
[0127] FAS was employed to measure treatment effects on motor performance. A 5×4 mixed ANOVA revealed a main effect of treatment (F3,26=12.00, p=0.00) and day (F4,104=23.79, p=0.00), with a significant interaction (F12,104=2.28, p=0.013). As shown in
[0128] Vilazodone modifies DA and 5-HT content in L-DOPA-primed and treated rats: Rats in Experiment 1 were killed 60 min post-L-DOPA and tissue was processed for HPLC analysis to determine the effects of treatment on striatal and raphe tissue neurochemistry. In striatum, a 2×4 (Lesion×Treatment) ANOVA revealed main effects of lesion on DA (F1,52=922.23, p=0.00), DOPAC (F1,52=696.86, p=0.00), DA turnover (F1,52=24.78, p=0.00), 5-HT (F1,52=6.54, p=0.014), 5-HIM (F1,52=31.34, p=0.00), and 5-HT turnover (F1,52=51.92, p=0.00), a main effect of treatment on DA turnover (F3,52=2.82, p=0.048), 5-HIM (F3,52=9.83, p=0.00), and 5-HT turnover (F3,52=6.15, p=0.001), and an interaction of lesion by treatment on DA turnover (F3,52=4.25, p=0.009; Table 1). Planned comparisons revealed that both doses of Vilazodone significantly increased DA in the lesioned striatum compared to groups receiving Vilazodone vehicle (0 mg/kg; all p<0.05), without altering significant L-DOPA-induced decreases in DA turnover (all p<0.05). Vilazodone (20 mg/kg) attenuated 5-HIM compared to all other treatments (all p<0.05) and reduced 5-HT Turnover compared to Vilazodone (0 mg/kg)+L-DOPA (0 mg/kg) (p=0.026).
TABLE-US-00002 TABLE 1 Treatment DA 5-HT Structure (mg/kg) DA* DOPAC* Turnover* 5-HT* 5-HIAA* Turnover* Striatum Intact VZD(0) + LD(0) 4584 ± 354 3310 ± 207 0.73 ± 0.03+ 366 ± 47.8 706 ± 62.8 2.02 ± 0.12+ VZD(0) + LD(6) 4561 ± 210 4051 ± 224 0.89 ± 0.04 241 ± 29.6{circumflex over ( )} 650 ± 68.9 2.77 ± 0.15+ VZD(10) + LD(6) 4827 ± 222 4138 ± 332{circumflex over ( )} 0.85 ± 0.05 326 ± 24.3 470 ± 35.5{circumflex over ( )}# 1.45 ± 0.04 VZD(20) + LD(6) 4560 ± 337 3839 ± 306 0.84 ± 0.03 305 ± 33.5 422 ± 38.6{circumflex over ( )}# 1.41 ± 0.07 Lesion VZD(0) + LD(0) 40.2 ± 7.26 93.7 ± 8.35 3.50 ± 1.43+ 245 ± 32.3 1047 ± 54.9 5.12 ± 0.99 VZD(0) + LD(6) 83.3 ± 7.99 .sup. 127 ± 30.7 1.42 ± 0.23 264 ± 39.6 907 ± 82.5 3.93 ± 0.63 VZD(10) + LD(6) 128 ± 14.2{circumflex over ( )}# .sup. 193 ± 20.5{circumflex over ( )} 1.56 ± 0.18 261 ± 30.7 842 ± 78.8 3.38 ± 0.31 VZD(20) + LD(6) 128 ± 26.2{circumflex over ( )}# .sup. 190 ± 44.6 1.50 ± 0.15 223 ± 23.8 596 ± 55.1+ 2.84 ± 0.33{circumflex over ( )} DorsalRaphe VZD(0) + LD(0) 48.6 ± 2.87+ .sup. 248 ± 29.8+ 5.06 ± 0.57 4448 ± 494.sup. 2643 ± 387.sup. 0.58 ± 0.04 Nucleus VZD(0) + LD(6) .sup. 112 ± 10.7 1114 ± 211 10.3 ± 1.76{circumflex over ( )} 5457 ± 786.sup. 2778 ± 261.sup. 0.57 ± 0.09 VZD(10) + LD(6) .sup. 153 ± 22.9 1175 ± 234 7.60 ± 0.78 7013 ± 462{circumflex over ( )}.sup. 2541 ± 187.sup. 0.37 ± 0.02{circumflex over ( )}# VZD(20) + LD(6) .sup. 128 ± 15.2 909 ± 186 7.19 ± 1.12 5946 ± 776.sup. 1985 ± 345.sup. 0.32 ± 0.03{circumflex over ( )}#
[0129] L-DOPA (LD)-primed rats were treated with Vilazodone (VZD; 0, 10, or 20 mg/kg, s.c.) 5 min prior to LD (0 or 6 mg/kg, s.c.) for 22 days. On the final treatment day, animals were sacrificed 60 min post-LD treatment and left and right striata and dorsal raphe nucleus (DRN) were collected for high performance liquid chromatography (HPLC) analysis. Values (means±standard mean error) are expressed as picograms of monoamine or metabolite per milligram wet tissue weight. Turnover estimates were determined by dividing a subjects metabolite value by its corresponding monoamine. Differences were assessed by a 2×4 (lesion×treatment) ANOVA, followed by Fisher's LSD post-hocs when appropriate. *p<0.01 vs lesion vs intact; +p<0.05 vs all; {circumflex over ( )}p<0.05 vs VZD (0)+LD (0); #p<0.05 vs VZD (0)+LD (6)
[0130] Analyses of the DRN revealed a main effect of treatment on DA (F3,26=9.88, p=0.00), DOPAC (F3,26=5.86, p=0.003), DA Turnover (F3,26=3.66, p=0.025) and 5-HT Turnover (F3,26=6.94, p=0.001). Post-hoc analysis revealed that L-DOPA treatment significantly increased DRN levels of DA, DOPAC and DA Turnover (all p<0.05), while only DA Turnover was attenuated in animals treated with Vilazodone (10 or 20 mg/kg). Interestingly, planned comparisons revealed Vilazodone (10 mg/kg) significantly increased DRN 5-HT while both doses of Vilazodone attenuated 5-HT Turnover (all p<0.05).
[0131] Experiment 2-Chronic Vilazodone blocks LID development: In Experiment 2, the ability of Vilazodone to prevent LID development was assessed. As shown in
[0132] Analyses of FAS revealed main effects of day (F4,136=32.89, p=0.00), treatment (F3,34=23.01, p=0.00) a significant interaction of day and treatment (F12,136=6.66, p=0.00;
[0133] L-DOPA-induced striatal c-Fos, PPD and PPE, up-regulation is attenuated by Vilazodone: All animals in Experiment 2 were killed on treatment and striatal tissue was analyzed with Real Time RT-PCR to determine if Vilazodone alters dyskinesia-associated striatal gene expression. A 2×4 (Side×Treatment) ANOVA revealed similar effect patterns in striatal PPD and c-Fos (
[0134] Experiment 3-Vilazodone works via 5-HT1AR but not 5-HT1BR: Pharmacologic antagonism of 5-HT1Rs was used to understand Vilazodone anti-LID efficacy. Testing 5-HT1AR contributions, analyses of summed ALO scores revealed a main effect of treatment on LID (χ2, (3)>17.76, p=0.0005;
[0135] When investigating 5-HT1BR, a main effect of treatment (χ2 (3)=15.45, p=0.001;
[0136] Discussion
[0137] Aberrant serotonergic neurotransmission is associated with LID, yet loss of L-DOPA efficacy and side effects have hindered clinical translation of 5-HT compounds. Independently, SERT or 5-HT1AR convey beneficial anti-dyskinetic effects, but prior studies have not simultaneously targeted both sites to optimize their application. Vilazodone, acting through both SERT and 5-HT1AR, dramatically reduces LID development and expression, while maintaining or with chronic administration even improving the pro-motor benefits of L-DOPA. Moreover, chronic Vilazodone attenuates aberrant striatal monoamine signaling, c-Fos mRNA, and PPD mRNA associated with LID.
[0138] Throughout chronic Vilazodone treatment, LID and motor performance were evaluated to characterize potential therapeutic efficacy. In L-DOPA-primed and L-DOPA-naïve rodents, Vilazodone treatment stably reduced AIMs. Within a week of treatment Vilazodone 10 mg/kg also facilitated stepping improvements, the effective pre-clinical anti-depressant dose (Oosting et al., 2016). While other 5-HT compounds suppress experimental LID, loss of L-DOPA efficacy, 5-HT side effects, have hindered therapeutic application (Conti et al., 2014; Fidalgo et al., 2015; Iravani et al., 2006; Lindenbach et al., 2015). Compound selectivity, dose, and timing relative to L-DOPA treatment produce variable and limited clinical efficacy (Dell'Agnello et al., 2001; Mazzucchi et al., 2015). With respect to 5-HT strategies, timing is of importance. Similar to SSRIs like citalopram and paroxetine, Vilazodone treatment acutely suppressed L-DOPA-induced motor improvements (Conti et al., 2014; Fidalgo et al., 2015), but this was transient and side effects, like 5-HT syndrome, weren't observed at any time point (Lindenbach et al., 2015). Indeed, effective adjuvant strategies will require chronic administration, which will change 5-HT neurotransmission. For example, emerging improvements in stepping may have occurred through 5-HT1AR desensitization (Ashby Jr et al., 2013; El Mansari et al., 2015). Thus, it is possible that chronic Vilazodone stimulation of DRN 5-HT1ARs normalized DA efflux without compromising movement as previously seen (Iravani et al., 2006; Goetz et al., 2007; Bezard et al., 2013).
[0139] Striatal neurochemistry was assayed after chronic treatment to isolate Vilazodone's mechanism of action. Traditional SSRI and 5-HT1A agonist effects on LID have been attributed to both short-term alterations in DAergic neurotransmission and long-term neuroplasticity. Prior work has shown that in dyskinetic rat, chronic citalopram-L-DOPA co-administration increases striatal DA over L-DOPA treatment alone (Conti et al., 2014). The 5-HT1AR agonist buspirone reportedly normalizes extracellular DA following L-DOPA administration (Politis et al., 2014). In the current work, HPLC analysis revealed that, as expected, 6-OHDA MFB lesions significantly depleted striatal DA values, but only Vilazodone-L-DOPA co-treatment significantly increased striatal DA, compared to vehicle, similar to experiments with citalopram (Conti et al., 2014). Although these effects were observed in post-mortem tissue, Kannari et al. (2006) demonstrated that in vivo local SERT blockade led to increased extracellular striatal DA after L-DOPA treatment. This effect contributes to the observed maintenance of L-DOPA's motor facilitation with chronic Vilazodone administration. Of note, L-DOPA also resulted in significantly increased levels of DA and DOPAC in the DRN, reflecting the global compensatory role of 5-HTergic neurons in taking up L-DOPA and converting it to DA for release (Arai et al., 1996; Navailles et al., 2010; Eskow-Jaunarajs et al., 2012). Importantly, Vilazodone also had significant effects on 5-HT neurotransmission, significantly attenuating striatal 5-HIAA and reducing DRN 5-HT turnover. These results suggest reduced 5-HT reuptake and metabolism, a common feature of chronic SSRI administration in rodents, non-human primates, and humans (De Bellis et al., 1993; Honig et al., 2009; Kreiss and Lucki, 1995; Smith et al., 2000). In fact Vilazodone-induced elevations in raphe 5-HT levels may elicit anti-dyskinetic actions of endogenous 5-HT at raphe 5-HT1AR autoreceptors (Eskow et al., 2009). The 5-HT1AR antagonist WAY100635 partially reversed the anti-LID efficacy of Vilazodone and other SSRIs, like citalopram (Conti et al., 2014). While a prior work suggests that co-stimulation of 5-HT1AR and 5-HT1B receptors can synergistically reduce LID (Munoz et al., 2008; Svenningsson et al., 2015), Vilazodone, despite increasing endogenous 5-HT, doesn't seem to act through this mechanism. NAS-181, a selective 5-HT1B antagonist, failed to reverse Vilazodone's anti-dyskinetic effects.
[0140] c-Fos mRNA increased in the dorsal striatum following chronic L-DOPA treatment (Bishop et al., 2009; Lopez et al., 2001; Mura et al., 2002). Vilazodone attenuated this dyskinesiogenic c-Fos induction, a key feature of anti-dyskinetic compounds (Bishop et al., 2009; Cenci, 2002; Lindenbach et al., 2011). Since c-Fos does not provide striatal pathway specificity, the opioid pre-cursors PPD and PPE, associated with D1R direct and D2R indirect pathway activity, respectively, were also measured (Gerfen et al., 1990; Sgroi et al., 2016). LID is associated with D1R direct pathway overactivity and increased PPD mRNA expression is consistently observed in DA-lesioned, L-DOPA-treated striata (Aubert et al., 2005; Bishop et al., 2009; Gross et al., 2003; Lindenbach et al., 2011; Tamim et al., 2010). L-DOPA (6 mg/kg) increases striatal PPD, compared to sham animals, and, more importantly is normalized by Vilazodone co-treatment. The role of D2R-coupled indirect pathway activity in LID is less clear (Politis et al., 2017). Striatal DA loss is associated with increased striatal PPE mRNA expression in rodents and non-human primates and this is exacerbated in the off-state following L-DOPA priming (Ravenscroft et al., 2004; Tamim et al., 2010). DA-lesioned L-DOPA-treated rats displayed striatal PPE mRNA upregulation. Only Vilazodone (20 mg/kg) attenuated this increase, suggesting an indirect pathway-independent mechanism for reducing LID. While Vilazodone significantly altered markers of striatal activation, it did not affect striatal 5-HT1AR mRNA. However, Real-Time RT-PCR does not measure protein levels or cellular localization so Vilazodone may alter 5-HT1AR expression post-transcriptionally, or alter receptor trafficking and sensitivity.
[0141] SERT and 5-HT1AR are well-characterized anti-dyskinetic targets. Vilazodone's unique pharmacologic profile predicted robust anti-LID efficacy. Vilazodone-L-DOPA co-administration significantly suppressed LID development and expression over a 3-week period with minimal effects on L-DOPA-induced motor facilitation. Vilazodone reduces LID via multiple points of neural articulation. Neurochemically, Vilazodone appears to maintain striatal L-DOPA-derived DA while augmenting aberrant signaling via endogenous 5-HT1AR neurotransmission, providing pro-motor and anti-dyskinetic effects coincidently. Such actions modulate downstream dyskinesia-associated striatal gene expression.
Example 2
[0142] 6-OHDA-induced dopaminergic lesions: Adult male Sprague-Dawley rats were randomly assigned to groups receiving either a 6-OHDA or sham lesion. All rats received desipramine (10 mg/kg i.p.) 30 min prior to surgery to prevent the lesioning of norepinephrine neurons by 6-OHDA. A single injection of 6-OHDA (8 μg of 6-OHDA free base in 4 μl of 0.1% ascorbic acid) was delivered into the right medial forebrain bundle (coordinates taken from bregma: −4.3 mm AP, 1.6 mm ML, −8.3 mm DV). The same experimental treatment was performed for the sham-operated rats using vehicle (4 μl of 0.1% ascorbic acid). A standard stepping test was used to evaluate the effectiveness of the 6-OHDA lesion. Rats exhibiting a significant lesion-induced decrease in adjusting steps (>75%) in the forelimb contralateral to the lesion were selected for further study. [Altwal 2019].
[0143] Unilateral 6-OHDA-lesioned rats modeling PD were treated with either vehicle and L-DOPA (5.0 mg/kg), vilazodone (10 mg/kg) and L-DOPA (5.0 mg/kg), or escitalopram (12.5 mg/kg) and L-DOPA (5.0 mg/kg). Rats were treated for 5 consecutive days/week (Mon-Fri), for 2 weeks. On the second day of each week, stepping tests were performed prior to drug administration, and 60 minutes post L-DOPA treatment.
[0144] All rats were evaluated three times per week for the presence of L-DOPA-induced dyskinesias (LIDs) (Wed-Fri) and once for the stepping test (Tue). LIDs were videotaped (2 min) at 30 min intervals (30-180 min) post-injection. Scores were given over 1 min epochs and classified as axial, limb, orolingual and locomotive.
[0145] The severity of each LID was scored using a standard scale. Each dyskinetic behavior was given an intensity and frequency score, which were then multiplied. The average sum of the products of each LID was determined in each animal. These data were then analyzed using a two-way RM ANOVA with a Tukey post-hoc test and outcomes exhibiting p-values of <0.05 were considered significant.
[0146] Escitalopram pretreatment significantly reduced LIDs severity following L-DOPA injection of 5 mg/kg. However, it affected L-DOPA therapeutic efficacy. Vilazodone pretreatment had the most significant effect on attenuating LIDs in 6-OHDA lesioned rats, and had no effects on forelimb akinesia or L-DOPA-induced prokinetic effects. Blocking the 5-HT1A partial agonism property of vilazodone using WAY100635 reverses the beneficial effects of vilazodone on LIDs. Vilazodone beneficial effects on LIDs is likely as a result of normalizing corticostriatal glutamatergic drive and GABAergic output of MSNs, as shown in electrophysiological studies.
[0147]
[0148]
[0149]
[0150]
Example 3
[0151] [Sellnow 2019] report that the hallmark motor symptoms in Parkinson's disease (PD) arise following substantial dopaminergic denervation within the striatum. Denervation results from the death of tyrosine hydroxylase (TH) expressing DA neurons of the substantia nigra pars compacta (SNc) as the disease progresses [Hoehn 1967, Kordower 2013]. The lack of proper DA signaling to the striatum creates an imbalance of the basal ganglia motor circuit, thus, causing bradykinesia, rigidity, tremor, and gait problems characteristic of PD [Goldman 2014]. Current treatment strategies, while not able to affect disease progression, are aimed at treating these primary motor symptoms. Since the late 1960s, L-3,4-dihydroxyphenylalanine (levodopa or L-DOPA) has been used as a catecholamine replacement therapy to alleviate motor symptoms [Cotzias 1967]. L-DOPA remains the gold-standard pharmacological treatment for PD.
[0152] While effective initially, the therapeutic window of L-DOPA narrows with the continuous loss of SNc neurons as the disease progresses, and higher doses are required to maintain the anti-akinetic effects of L-DOPA. Moreover, chronic treatment with L-DOPA leads to the development of L-DOPA-induced dyskinesias (LID), a series of motor symptoms distinct and independent from the PD motor deficits being treated (reviewed in [Bastide 2015]). These symptoms, comprised of painful and disrupting movements including hyperkinesia, dystonia, and chorea, occur in a majority of PD patients, developing in up to 50% of patients within 5 years of beginning treatment, and up to 90% of patients within 10 years [Ahlskog 2001, Manson 2012].
[0153] Studies show that LID development is a multifaceted process. However, it is largely agreed upon that the intermittent oral dosing of L-DOPA results in large variations in extracellular DA. Ultimately, this pulsatile release of DA, together with the denervated state of the striatum, results in maladaptive molecular and structural changes in the DA-responsive neurons of the striatum, specifically medium spiny neurons (MSNs), leading to altered basal ganglia signaling (reviewed in [Cenci 2010]). Given the extreme nigrostriatal denervation at the time of diagnosis [Kordower 2013], the actual source of striatal DA following L-DOPA administration has been debated over the past half century. The leading hypothesis is that uptake of L-DOPA and its subsequent dysregulated metabolism to DA, and release by serotonergic 5-hydroxytryptamine (5-HT) neurons in the dorsal raphe nucleus (DRN) may be linked to dyskinesogenesis (reviewed in [De Deurwaerdere 2016]). These neurons express aromatic L-amino acid decarboxylase (MDC) and can therefore convert L-DOPA into DA. However, DRN neurons do not express the regulatory mechanisms to monitor and control DA synthesis and release into the synapse, allowing for the unregulated release of DA into a hypersensitized striatum [Maeda 1999]. Additionally, serotonergic innervation of the striatum increases substantially following DA denervation, allowing the majority of L-DOPA to be metabolized and released as DA by serotonergic terminals [Lundblad 2002, Maeda 2005, Roussakis 2016, Rylander 2010, Yamada 2007]. This overwhelming exposure of the DA-depleted striatal MSNs to exogenous DA is hypothesized to be a large contributor to LID. In fact, studies in rats show that specifically lesioning the DRN [Carta 2007, Eskow 2009] or co-administering L-DOPA with 5-HT1 receptor agonists [Bezard 2013, Ghiglieri 2016, Meadows 2017, Politis 2014], effectively reduces or eliminates LID.
[0154] Normal regulation of DA signaling is mediated pre-synaptically primarily through the DA active transporter (DAT) and the DA autoreceptor. DAT directly regulates the levels of DA in the synapse by transporting synaptic DA back into the terminal. The dopamine autoreceptor (D2Rs) is an isoform of the D2 DA receptor (D2RL) missing 29 amino acids from the third intracellular loop [Dal Toso 1989]. D2Rs detects synaptic DA levels and regulates DA signaling in three ways, 1) by downregulating DA production through TH regulation, 2) regulation of reuptake through DAT, and 3) by directly inhibiting DA release (reviewed in [Ford 2014]). Each of these modes of action are mediated through the inhibitory Gi alpha protein signaling pathways following D2Rs activation.
[0155] These canonical G-protein-coupled receptor (GPCR) signaling pathways similarly inhibit serotonergic signaling in DRN neurons through 5-HT1 autoreceptor activation [Harrington 1988, Okada 1989]. Previous studies using 5-HT1 agonists show promising reductions in LID. Unfortunately, these drugs can negate the anti-parkinsonian therapeutic benefits of L-DOPA animal models, and in some cases worsen PD symptoms in clinical trials [Cheshire 2012, Iravani 2006, Kannari 2002, Olanow 2004].
[0156] While current evidence suggests a crucial role of serotonergic input and activity in LID, direct evidence of the abnormal dopaminergic neurotransmission and dysregulated DA release is lacking. Unequivocal evidence is provided for the role of serotonergic DA neurotransmission in dyskinesogenesis and examine a novel therapeutic approach of modulating this non-physiological adaptation in the parkinsonian brain. To do this, serotonergic neurons were provided with DAergic regulatory mechanisms by ectopically expressing the D2Rs autoreceptor in the DRN of parkinsonian 6-OHDA lesioned rats, and evaluated the effect of ectopic D2Rs activity on L-DOPA efficacy, LID formation, response to DA agonists, and striatal DA release.
[0157] Adeno-associated virus production: The D2Rs and GFP coding sequences were cloned into MV genomes under the control of the chicken β-actin/cytomegalovirus (CBA/CMV) promoter for ubiquitous and robust expression. MV 2/9 was produced via triple-transfection of HEK 293T cells with the genome and helper plasmids. Virus was recovered from cells using freeze-thaw cycles, purified using an iodixanol gradient (Optiprep Density Gradient Sigma-Aldrich, St Louis, Mo.), followed by buffer exchange and concentration using concentrator columns (Orbital Biosciences, Topsfield, Mass.) as described previously [Benskey 2016 (B)]. The viral titer was determined using digital droplet PCR (ddPCR) and normalized to 1×10.sup.13 vector genomes (μg)/ml using Balanced Salt Solution (Sigma-Aldrich, St Louis, Mo.).
[0158] Animals and surgeries: Studies were performed using adult male Fischer F344 rats (200-220 g upon arrival; Charles River, Wilmington, Mass.).
[0159] All 6-OHDA and vector surgeries were performed under 2% isoflurane. After being anesthetized, animals were placed in a stereotaxic frame and were injected using a glass capillary needle fitted to a Hamilton syringe (Hamilton, Reno, Nev.) [Benskey 2016 (A)]. Three weeks following lesion surgery, animals were tested for spontaneous forepaw use (cylinder test) to estimate lesion efficacy. Vector treatment groups were normalized using forepaw deficits in order to ensure equal lesions between the treatment groups.
[0160] For lesion surgeries 5 mg/ml 6-OHDA hydrobromide (Sigma-Aldrich, St. Louis, Mo.) was prepared in 0.2 mg/ml ascorbic acid immediately prior to the injections. Animals received 2μl injections of 6-OHDA into the medial forebrain bundle (MFB) (from bregma: Anterior Posterior (AP) −4.3 mm, Medial Lateral (ML)+1.6 mm, Dorsal Ventral (DV) −8.4 mm from skull) and the SNc (from bregma: AP −4.8 mm, ML+1.7 mm, DV −8.0 mm from skull), for a total of 10 μg 6-OHDA per site and 20 μg per animal. The glass needle was lowered to the site and the injection started after 30s. 6-OHDA was injected at a rate of 0.5 μl/minute. The needle was removed 2 minutes after the injection was finished and cleaned between each injection.
[0161] Vector delivery was performed 3 weeks following the 6-OHDA lesion via stereotaxic delivery [Benskey 2016 (A)]. A subset of animals (N=7) destined for electrophysiological measures did not receive a 6-OHDA lesion. Using the same procedure as described for the lesion surgeries, animals received a single midline 2 μl injection of virus (AAV2/9-DRs, 1×10.sup.13 μg/ml; AAV2/9-GFP, 1×10.sup.13 μg/ml) to the DRN (from bregma: AP −7.8, ML −3.1, DV −7.5 from skull). The stereotaxic arm was positioned in a 30° lateral angle in order to avoid the cerebral aqueduct.
[0162] Rats were tested for baseline forepaw adjusting steps. Thereafter, microdialysis cannulation surgery was performed under 2-3% isoflurane in oxygen with the tooth bar set to 5 mm below the interaural line. Five minutes before surgery and 24 h after surgery rats received an injection of Buprinex (0.03 mg/kg, i.p.). A unilateral dorsal striatal-directed cannula (CMA 12 Elite; Stockholm, Sweden) was implanted ipsilateral to lesion (from bregma AP: 1.2 mm; ML: −2.8 mm; DV: −3.7 mm). The cannula was fixed in place by four jeweler's screws, jet liquid, and dental acrylic (Lang Dental, Wheeling, Ill.). Two weeks following cannulation surgery, rats underwent behavioral testing.
[0163] Burr holes (˜1 mm in diameter) were drilled in the skull overlying the DRN of non-lesioned rats. Prior to experimentation all animals were anesthetized with urethane (1.5 g/kgi.Math.p.) and placed in a stereotaxic apparatus. Bipolar stimulating/recording electrodes were implanted in the frontal cortex and DRN on the right side using a micromanipulator (coordinates from Bregma: AP: 3.2 mm; ML: 0.8 mm lateral; DV: 4.4 mm ventral (frontal cortex) or AP: 7.8 mm; ML 3.1 mm; DV: 7.5 mm with the manipulator angled 30 degrees toward Bregma) as previously described [Chakroborty 2017].
[0164] Abnormal involuntary movement (AIM) ratings and drug treatments: Animals were allowed to recover for 3 weeks following vector injections, and to allow for peak expression of the viral transgene [Reimsnider 2007]. After this time, L-DOPA treatment and abnormal involuntary movement (AIM) scale ratings began (see time line in
[0165] Animals received subcutaneous injections of L-DOPA/benserazide (Sigma-Aldrich, St Louis, Mo.) three times per week and were rated using the AIM scale in 25-min intervals post-injection until all LID behavior had subsided. L-DOPA doses ranged between 2 mg/kg-12 mg/kg (
[0166] Parkinsonian motor evaluation: To assess whether D2Rs viral therapy affects the anti-parkinsonian properties of L-DOPA therapy, parkinsonian motor behavior on and off L-DOPA was evaluated using the cylinder task and the forepaw adjusting steps (FAS) test. Rats with significant lesions perform poorly on both these tests, with impairment to the forepaw contralateral to the lesion that is alleviated with L-DOPA treatment [Chang 1999, Schallert 2006]. The cylinder task was conducted as previously reported [Manfredsson 2007]. Animals were placed in a clear Plexiglas cylinder on top of a light box for 5 to 7 minutes while being recorded. Each animal was rated by counting ˜20 weight-bearing forepaw placements on the cylinder (contralateral to the lesion, ipsilateral to the lesion, both) to determine the percentage use of the forepaw contralateral to the lesion, which is derived by dividing the sum of contralateral touches and half of both forepaw touches by the total forepaw touches, and multiplying this number by 100. Trials were performed following the initial L-DOPA treatment (AIM evaluation) period, and tested either off L-DOPA or, on the following day, 50 min after receiving a 6 mg/kg L-DOPA injection (12 mg/kg benserazide).
[0167] The FAS test was performed per [Meadows 2017]. Briefly, rats were restrained by an experimenter so that only one forepaw was free to touch the counter. Rats were then dragged laterally along a 90 cm distance over 10 s while a trained rater blind to the experimental condition counted the number of steps. Data are represented as forehand percent intact, which are derived by taking the number of steps taken by the contralateral forehand and dividing it by the ipsilateral forehand, and then multiplying this number by 100. The test was performed over 2 days either off L-DOPA or 60 min following an 8 mg/kg or 12 mg/kg L-DOPA injection.
[0168] Tissue collection: Two hours following the final L-DOPA administration, animals from the AIM experimentation were sacrificed via sodium pentobarbital overdose and intracardially perfused with Tyrode's solution (137 mM sodium chloride, 1.8 mM calcium chloride dihydrate, 0.32 mM sodium phosphate monobasic dihydrate, 5.5 mM glucose, 11.9 mM sodium bicarbonate, 2.7 mM potassium chloride). Brains were rapidly removed and coronally hemisected, with the rostral portion of the left and right striatum dissected out and flash frozen in liquid nitrogen for biochemical analysis. The caudal portion of the brain was postfixed for 72 h in 4% paraformaldehyde (PFA) in phosphate-buffered saline and then cryoprotected by saturation in 30% sucrose. Brains were frozen and sectioned coronally at 40 μm thickness using a sliding microtome into free floating sections and stored in cryoprotectant (30% ethylene glycol, 0.8 mM sucrose in 0.5× tris-buffered saline) until further use.
[0169] Immunohistochemistry: A 1:6 series of free-floating tissue was stained immunohistochemically for TH (MAB318, MilliporeSigma, Burlington, Mass.), D2R (AB5084P, MilliporeSigma, Burlington, Mass.), GFP (AB290, Abcam, Cambridge, United Kingdom), IBA1 (019-19,741, Wako Life Sciences, Richmond, Va.), or 5-HT (NT-102, Protos Biotech, New York, N.Y.) using methods previously reported [Benskey 2018]. Sections were washed in 1× Tris-buffered saline (TBS) with 0.25% Triton x-100, incubated in 0.3% H2O2 for 30 min, and rinsed and blocked in 10% normal goat serum for 2 h. Tissue was incubated in primary antibody (TH 1:4000, D2R 1:1000, GFP 1:20,000, IBA1 1:4000, 5-HT 1:10,000) overnight at room temperature. After washing, tissue was incubated in secondary antibody (biotinylated horse anti-mouse IgG 1:500, BA-2001; Vector Laboratories, Burlingame, Calif.; biotinylated goat anti-rabbit IgG 1:500, AP132B, Millipore-Sigma, Burlington, Mass.) followed by the Vectastain ABC kit (Vector Laboratories, Burlingame, Calif.). Tissue staining was developed with 0.5 mg/ml 3,3′ diaminobenzidine (DAB, Sigma-Aldrich, St. Louis, Mo.) and 0.03% H.sub.2O.sub.2. Sections were mounted on glass slides, dehydrated, and coverslipped with Cytoseal (ThermoFisher, Waltham, Mass.).
[0170] Tissue for immunofluorescence dual labeling of D2Rs or GFP with SERT (340-004, Synaptic Systems, Goettingen, Germany) were washed with 1×TBS with 0.25% Triton x-100, blocked in 10% normal goat serum for 2 h, and probed with primary antibody overnight (D2Rs 1:1000, GFP 1:20,000, SERT 1:300). Tissue was incubated with secondary antibody (A110081:500, A110761:500; ThermoFischer, Waltham, Mass.) in the dark for 2 hours, and washed in TBS before being mounted and coverslipped with Vectashield Hardset Antifade Mounting Medium (Vector Laboratories, Burlingame, Calif.). Images were taken on a Nikon Eclipse 90i microscope with a QICAM fast 1394 camera (fluorescence; QImaging, Surrey, British Columbia, Canada) or a Nikon D-1 camera (brightfield microscopy; Nikon, Tokyo, Japan). The figures were made using Photoshop 7.0 (Adobe, San Jose, Calif.) with the brightness, sharpness, and saturation adjusted only as needed to best represent the staining as it is viewed directly under the microscope.
[0171] In vivo microdialysis: As outlined above, a separate cohort of parkinsonian rats treated with GFP or D2Rs were utilized for in vivo microdialysis. The night before the procedure, striatal probes (CMA 12 Elite; membrane length=3 mm; 20,000 Da; Stockholm, Sweden) were inserted into the guide cannula so that they extended from bregma DV: −3.7 to −6.7 mm within the dorsal striatum. Rats underwent microdialysis at least 2 days following the last L-DOPA administration. During microdialysis, rats received intrastriatal infusion of filtered artificial cerebrospinal fluid (aCSF) (128 mM NaCl, 2.5 mM KCl, 1.3 mM CaCl.sub.2), 2.1 mM MgCl2, 0.9 mM NaH2PO4, 2.0 mM Na2HPO4, and 1.0 mM glucose, pH 7.4). Dialysate samples were collected every 20 min. Briefly, rats were habituated to microdialysis for 1 h. Fifty minutes into the procedure, rats received a subcutaneous injection of L-DOPA vehicle, which consisted of 0.9% NaCl, and 0.1% ascorbate. Rats then underwent baseline testing for 1 hour to determine baseline levels of monoamines prior to L-DOPA treatment. After that a new collection tube was used and 10 minutes later rats received an injection of L-DOPA (12 mg/kg+12 mg/kg Benserazide, s.c.). Samples were taken every 20 min for 3 h. Following the procedure, rats were removed from the microdialysis bowl and striatal probes were replaced with a dummy probe. At least 2 days after microdialysis, rats were sacrificed via rapid decapitation, the anterior striatum was taken for verification of cannula placement, the posterior striatum was taken for HPLC, and the hindbrain was placed in 4% PFA for 3 days before being placed in 30% sucrose in phosphate-buffered saline (PBS). Brains were shipped on ice in a 50 mL conical containing 30% sucrose in 0.1 M PBS to MSU.
[0172] High-performance liquid chromatography for monoamine tissue analysis: Striatal tissue and in vivo microdialysis samples were analyzed using HPLC. Reverse-phase HPLC was performed on striatal tissue samples as previously described [Kilpatrick 1986, Meadows 2017]. Briefly, tissue samples were homogenized in ice-cold perchloric acid (0.1 M) with 1% ethanol and 0.02% ethylenediaminetetraacetic acid (EDTA). Homogenate was spun at 4° C. for 45 min at 14,000 g. Supernatant was removed and, using an ESA solvent delivery system (Model 542; Chelmsford, Mass., USA) ESA autoinjector (Model 582), analyzed for levels of norepinephrine, 3,4-dihydroxyphenylacetic acid (DOPAC), DA, 5-hydroxyindoleacetic acid (5-HIAA), and 5-HT. Monoamines and metabolites were detected as a generated current as a function of time by EZCHROM ELITE software via a Scientific Software, Inc. (SS240x) Module. Data are displayed as peaks for monoamines and metabolites, which are compared to a standard curve made from monoamine and metabolite samples of known concentrations ranging from 1e-6 to 1e-9. Values were then normalized to tissue weight and lesion deficits are reported as percent depletion, which is equal to 100 (1-M Lesion/M Intact).
[0173] Dialysate samples were analyzed via reverse-phase HPLC on an Eicom HTEC-500 System (Amuza Inc., San Diego, Calif.). Briefly, 10 μL of each dialysate sample was analyzed for NE, DA, and 5-HT using an Eicompak CAX column maintained at 35° C. with a flow rate of 250 μL/min. Mobile phase (75 mM Ammonium acetate, 9.36 mM acetic acid, 1.33 mM EDTA, 0.94 mM Methanol, 50 mM sodium sulfate). Samples were compared to known concentrations of monoamines (100, 10, 1, 0.1, and 0.05 ng/μL dissolved in a potassium phosphate buffer (0.1 mM potassium phosphate monobasic, 0.1 mM ethylenediaminetetraacetic acid, 0.02 mM phosphoric acid), resulting in a final value of monoamine in ng/μL.
[0174] Total enumeration of TH+ and 5-HT+ neurons: Lesion severity was determined using total enumeration of TH-positive neurons in three representative sections within the SNc identified by the presence and proximity to the medial terminal nucleus (MTN) of the accessory optic tract at levels equivalent to −5.04 mm, −5.28 mm and −5.52 mm relative to bregma according to a previously validated method [Gombash 2014]. Briefly, the intact and lesion SNc were quantified for all TH immunoreactive cells using a 20× objective and MicroBrightfield StereoInvestigator software (MicroBrightfield Bioscience, Williston, Vt.). The total number of TH cells on the intact and lesioned hemispheres were averaged, and lesion efficacy was derived by dividing the lesioned hemisphere average by the intact hemisphere average and multiplying that value by 100.
[0175] Total number of 5-HT positive neurons in the DRN were also quantified with total enumeration [Gombash 2014]. Three sections of the DRN were quantified for all 5-HT immunoreactive cells under a 20× objective using MicroBrightfield StereoInvestigator (MicroBrightfield Bioscience, Williston, Vt.). The total number of from all three sections per animal were summed to give a total number of 5-HT neurons.
[0176] Electrophysiology: Recording microelectrodes were manufactured from 2.0 mm OD borosilicate glass capillary tubing and filled with sodium chloride (2M) solution. Electrode impedance was 5-15MΩ. The signal to noise ratio for all recordings was >4:1. The level of urethane anesthesia was periodically verified via the hind limb compression reflex and maintained using supplemental administration as previously described [Padovan-Neto 2015, Sammut 2010]. Temperature was monitored using a rectal probe and maintained at 37 C.° using a heating pad (VI-20F, Fintronics Inc., Orange, Conn.). Electrical stimuli (duration=500 μs, intensity=1000 μA) were generated using a Grass stimulator and delivered in single pulses (0.5 Hz) while searching for cells [Padovan-Neto 2015]. Once isolated, recordings consisted of basal (pre-drug), saline vehicle, and drug-treatment-(see below) induced changes in spike activity recorded in a series of 3 min duration epochs.
[0177] All compounds and physiological 0.9% saline were prepared daily and administered intravenously (i.v.) through the lateral tail vein to enable rapid examination of potential acute effects of vehicle or drug on DRN neuronal activity. The selective 5-HT1A agonist 8-OH-DPAT (5 μg/kg, i.v.), the selective 5-HT1A antagonist WAY100635 (100 μg/kg, i.v.), and the D2R agonist Quinpirole (500 μg/kg, i.v.) were dissolved in vehicle and administered systemically to either BFP or D2Rs rats. DRN 5-HT neuron activity was recorded prior to and immediately following drug administration as described above.
[0178] Statistical analysis: Statistical analysis was performed using Statview (version 5.0) or in SPSSversion 23 with a set to 0.05. All graphs were created in GraphPad Prism version 7.0 (GraphPad Software, La Jolla, Calif.) or Excel (Microsoft, Redmond, Wash.). Lesion status was evaluated using unpaired, one-tailed t-tests. AIMs were evaluated using a non-parametric Mann-Whitney U test, with p≤0.05 being considered statistically significant Bonferroni post-hoc tests were employed when significant main effects were detected. Cylinder and FAS data for forehand and backhand stepping were submitted to a mixed model ANOVA with within-subjects factors of treatment (2: Baseline, L-DOPA) and between-subjects factors of vector (GFP, D2R). Overall percent intact values for FAS were determined by taking the overall number of right paw steps divided by the number of left paw steps and multiplying the quotient by 100. Similarly, overall percent intact values were analyzed via a repeated-measures ANOVA with within-subjects factor of treatment and between subjects factor of vector. Monoamine content (as determined by HPLC) was submitted to a mixed-model ANOVA with within-subjects factor of treatment (2: Vehicle, L-DOPA) and between-subjects factor of vector. Fisher's least significant difference (LSD) post-hocs and planned paired-samples t-tests were employed as appropriate to clarify significant effects. Additionally, independent-samples t-tests were employed to reveal effects of vector on the timing of DA, NE, and 5-HT efflux. HPLC values for striatal tissue were submitted to a mixed-model ANOVA with within-subjects factor of side and between-subjects factor of vector. Subsequently, since DA depletion did not vary as a function of vector, values for each monoamine for each side were collapsed across treatments and compared via paired-samples t-tests. For electrophysiology experiments, the difference between the spontaneous and evoked electrophysiological activity of identified DRN-5-HT neurons across groups was determined and served as the dependent variable for analyses. A two-way repeated measures ANOVA (GFP vs. gene therapy (ectopic expression of the DA D2 AR in 5-HT DR neurons))×2 (vehicle vs. drug treatment) with a set to 0.05 and all “n's” adequately powered for electrophysiological studies was conducted using Sigma Stat software (San Jose, Calif.), and the potential two-way interaction effect was examined to determine how treatment effects differ as a function of drug treatment or gene therapy [Padovan-Neto 2015].
[0179] Validation of lesion and transgene expression: In order to assess if exogenous expression of D2Rs in the DRN could inhibit LID development or decrease LID severity, adult Fischer rats were rendered parkinsonian with 6-OHDA delivered to the SNc and MFB. Because LID is dependent on the severity of the lesion [Winkler 2002] sufficient nigrostriatal denervation was validated post mortem. Immunohistochemistry of the striatum (
[0180] After a three-week recovery period, rAAV 2/9 expressing either D2Rs or GFP was delivered by stereotaxic injection into the DRN. Following sacrifice, transduction was confirmed with immunohistochemistry (INC) of D2Rs or GFP (
[0181] D2Rs delivery to the dorsal raphe eliminates LID: After a 4-week recovery period to allow for optimal transgene expression [Reimsnider 2007], animals were treated with L-DOPA and rated for AIMs (see
[0182] D2Rs does not affect parkinsonian motor behavior: To assess if rAAV-D2Rs treatment alters the anti-akinetic properties of L-DOPA, motor behavior was examined using the cylinder task (
[0183] Dopamine receptor agonists do not induce significant AIMs in L-DOPA-primed rAAV-D2Rs rats: Next, it dopamine agonists were examined for their ability to induce AIMs in the rAAV-D2Rs treated rats that had remained resistant to LID after the L-DOPA dosing paradigm. Animals received three repeated doses each of a non-selective DA agonist (apomorphine, 0.1 mg/kg), a D2/3-specific receptor agonist (quinpirole, 0.2 mg/kg), and a D1-specific receptor agonist (SKF-81297, 0.8 mg/kg) and were evaluated for AIM severity (see timeline in
[0184] D2Rs expression in the dorsal raphe reduces striatal dopamine efflux following L-DOPA delivery: In order to determine if ectopic D2Rs expression in the DRN was inhibiting LID by moderating DA release from serotonergic neurons, a second cohort of animals was generated in order to perform in vivo microdialysis (rAAV-D2Rs n=6, rAAV-GFP n=7). Animals were lesioned and received vector in an identical manner to the first cohort, and subsequently treated with L-DOPA to establish LID. In order to determine differences between vector groups in the absence of L-DOPA, striatal dialysate was analyzed via HPLC and data for monoamine content were examined using a 2 (vector)×2 (treatment) mixed-model ANOVA. Overall, DA values were dependent upon treatment, F(1,11)=124.35, p<0.05, and vector, F(1,11)=7.39, p<0.05. Planned pairwise comparisons revealed that L-DOPA treatment increased striatal DA efflux in both groups. However, rats treated with the D2Rs viral vector had lower levels of DA efflux than did rats treated with the GFP vector (p<0.05) (
[0185] D2Rs expression inhibits 5-HT neuron activity: In order to demonstrate that the ectopically expressed D2Rs have the capacity to inhibit the activity of identified 5-HT neurons, electrophysiological recordings were performed on a separate cohort of (intact, non-L-DOPA-treated, non-dyskinetic) animals. Animals received a stereotaxic delivery of either vector as described above, and 4-12 weeks later in vivo single-unit extracellular recordings of DRN neurons were performed. Putative 5-HT neurons were identified based initially on their firing characteristics (e.g., long-duration action potentials, regular firing pattern interrupted with burst activity). Next, neurons were identified as serotonergic based on well characterized responses to systemic administration (i.v.) of 5HT1AR agonist (8-OH-DPAT) and reversal with antagonist (WAY-100635) which restored 5-HT neuron firing to that of baseline (
[0186] Conclusions
[0187] rAAV was used to ectopically express the dopamine autoreceptor (D2Rs) in order to equip DRN 5-HT neurons with a DA-mediated autoregulatory mechanism. Dysregulated DA release from 5-HT neurons through a phenomenon known as “false neurotransmission” has been extensively implicated as a key contributor to LID development [Bibbiani 2001, Carta 2007, Eskow 2009, Maeda 2003, Maeda 1999, Maeda 2005, Munoz 2009]. The data demonstrate that providing DA-dependent autoregulation in 5-HT neurons can prevent LID formation, thus, providing unambiguous evidence that 5-HT neurons play a central role in DA-dependent symptomology.
[0188] Studies supporting the serotonin hypothesis of LID suggest that DA synthesis and release from striatal 5-HT terminals is involved in AIM presentation. Specifically, studies ablating DRN neurons or dampening their activity with serotonin autoreceptor agonists have been shown to reduce or eliminate LID; the hypothesized reasoning being that reducing aberrant serotonergic neuronal activity following L-DOPA administration leads to a reduction in striatal DA release from ectopically sprouted DRN terminals [Carta 2007, Eskow 2007, Iravani 2006]. Although the mechanism by which 5-HT neurons process L-DOPA and release DA is not fully established, it is well known that the synthesis and vesicular packaging mechanisms are present in serotonergic neurons [Arai 1995, Gantz 2015, Tanaka 1999].
[0189] When DRN are induced to ectopically express D2Rs autoreceptors, 1) hyper-DA release in the striatum following L-DOPA is significantly dampened, presumably by providing DA-dependent autoregulation in striatal 5-HT terminals and 2) that this approach can completely prevent LID formation without compromising motor benefit. These data provide unambiguous evidence that 5-HT neurons play a central role in the DA-dependent pathophysiology of LID.
[0190] Dopamine autoregulation in the dorsal raphe blocks 5-HT neuron activity and LID development: Expressing DA regulatory factors in 5-HT neurons decreases LID severity. 5-HT autoreceptors share a canonical signaling cascade with the D2-type DA autoreceptors—both are inhibitory G-protein coupled receptors (GPCRs) that reduce cellular cAMP to inhibit neuronal signaling [Harrington 1988, Neve 2004]. Ectopically expressing the DA autoreceptor D2Rs in DRN neurons can serve a physiological autoregulatory function. D2Rs autoreceptor expression in the DRN of naïve mice results in a reduction of 5-HT-mediated currents [Gantz 2015]. Direct recordings of single 5-HT neurons in the DRN reveal that ectopic D2Rs expression can provide an inhibitory neuromodulatory effect in 5-HT neurons, characterized by a strong decrease in spontaneous firing following systemic DA D2R agonist administration. rAAV targeted to the DRN in hemiparkinsonian rats that subsequently received a LID-inducing dosing regimen of L-DOPA, revealed that DRN expression of D2Rs provided complete protection against the development of LID, an effect that also persisted at high doses of L-DOPA. There was no difference in the extent of nigrostriatal denervation between the groups, nor was there any demonstrable toxicity due to either treatment in the DRN. Thus, prevention of LID was explicitly due to expression of D2Rs in the DRN.
[0191] Dopamine efflux into the striatum is reduced with dorsal raphe D2Rs expression: Although there is a wealth of research supporting the abnormal serotonergic input in LID development [Arai 1995, Nicholson 2002, Scholtissen 2006], direct evidence showing that the contribution is due to an increase in DA release from these neurons is limited. Using in vivo microdialysis, L-DOPA mediated DA efflux into the striatum was shown to be significantly modulated by negatively regulating DRN serotonin neurons with D2Rs expression. A total blockade of LID development was observed with a partial reduction in DA efflux in the striatum. This indicates that a complete block of DA signaling in the striatum is not required for LID inhibition, but rather, mitigation of the pulsatile DAergic tone that occurs with oral administration of L-DOPA is required. Additionally, achieving a total depletion of DA release in the striatum would likely result in a loss of L-DOPA efficacy, as the primary source of L-DOPA metabolism and DA release in severely DA denervated animals originates from DRN neurons. The data suggests that partial DA efflux reduction and proper DAergic regulation is sufficient to ameliorate LID in the animal model.
[0192] In contrast to DA efflux, there was no evidence of decreased 5-HT efflux in the striatum in rAAV-D2Rs animals, suggesting 5-HT release was not affected. This is surprising given the finding that autoreceptor stimulation effectively reduces 5-Ht neuron firing. One likely explanation for this observation is that the lack of impact on striatal 5-HT release was due to a lack of direct stimulation of 5-HT release concomitant with L-DOPA treatment, thus, the measurements reflected baseline 5-HT release. Nevertheless, the findings demonstrate that D2Rs can induce DAergic regulation in 5-HT neurons, by ‘hijacking’ endogenous signaling cascades and reducing neuronal activity following L-DOPA administration.
[0193] The in vivo electrophysiology and microdialysis data together suggest that the mechanism by which expression of D2Rs in DRN neurons provides complete protection against the development of LID is through a neuromodulatory feedback mechanism. This is further supported based on equal levels of nigral DA neuron loss between rAAV-D2R and rAAV-GFP groups, supporting that this antidyskinetic efficacy was explicitly due to expression of D2Rs in the DRN.
[0194] The data indicate that exogenously provided D2Rs can couple with Gαi subunits in DRN neurons, and induce the appropriate signaling cascades to reduce neuronal activity in the presence of exogenous L-DOPA. In conjunction with the LID studies utilizing serotonin agonists, the data confirm that reducing the activity of the serotonin system can dramatically inhibit LID. However, the critical advantage of this target-specific gene therapy approach over pharmacological therapy [Cheshire 2012, Iravani 2006, Kannari 2002, Olanow 2004] is that there is no decrease in motor benefit of L-DOPA. Serotonergic neurons, when supplied exogenously with a single DA-regulatory factor, can modulate DA release and completely prevent the induction of LID in a ‘prevention’ scenario.
[0195] Ectopic D2Rs Expression in the Dorsal Raphe Blocks: L-DOPA Priming in the Striatum
[0196] In order to better understand the global impact of striatal DA regulation via DRN D2Rs expression on an array of DA therapies in parkinsonian subjects, the hypothesis that the protective effects of this autoreceptor treatment would be negated in the presence of DA-receptor agonists which directly bind to DA receptors on striatal medium spiny neurons (MSNs) was tested. Since the DA regulation thru the D2Rs is a presynaptic mechanism, treatment with DA receptor agonists, which act at postsynaptic receptors that become supersensitive with striatal DA depletion and result in dyskinesias in animal models and patients [Boraud 2001, Boyce 2001, Chondrogiorgi 2014, Gomez-Mancilla 1992], should induce AIMs in rAAV-D2Rs-treated animals resistant to LID. Treatment with D1-, D2-specific, or pan-DA agonists did not induce severe AIMs in rAAV-D2Rs animals, and only a mild-to-modest dyskinetic response was seen with the D1 agonist SKF-81297, the last of the three DA agonist drugs tested. This suggests that D2Rs therapy disallowed LID priming to occur in striatal MSNs. The autoreceptor allows for proper regulation of DA signaling from DRN neurons, removing the pulsatile stimulation induced by intermittent DA dosing which is important in LID development Thus, the MSNs of rAAV-D2Rs treated animals first exposure to abnormal DA signaling would be at the initial agonist challenge, where priming could begin. This increase in AIMs behavior with the D1 agonist may have been due to a mild degree of DA-agonist induced priming, a phenomenon that is to be expected as direct MSN DA receptor activation would not be mitigated by DRN D2Rs expression. This is supported by the experimentation by Carta and colleagues, where the co-administration of apomorphine with the 5-HT1A agonist after an induction period where L-DOPA was administered over 3 weeks, did not alleviate LID, suggesting that the induction phase irreversibly primed the neurons to LID [Carta 2007].
[0197] It is well established that LID development is associated with a “priming-period” consisting of discontinuous, non-physiological, striatal DA tone that results in morphological and molecular changes to the MSNs [Carta 2003, Cenci 2010, Morelli 1989, Pinna 1997, Simola 2009, Steece-Collier 2009, Zhang 2013]. The data therefore indicates that D2Rs-treated animals were blocked from the L-DOPA priming by counteracting the non-physiological surges of DA release, thereby preventing a host of pathological molecular mechanisms that may include normalizing postsynaptic striatal DA receptor supersensitivity. The fact that at the end of the treatment a mild-to-moderate increase in AIM presentation in rAAV-D2Rs animals with DA agonist treatment was observed as compared to L-DOPA, suggest that these animals were in the early stages of priming, a phenomenon that is to be expected as direct MSN DA receptor activation would not be mitigated by DRN D2Rs expression. While there was a break between L-DOPA and DA agonist treatment (
[0198] Inhibition of dorsal raphe serotonergic neurons does not mitigate the anti-parkinsonian benefits of L-DOPA: As briefly discussed above, it was important to confirm that D2Rs expression in the DRN does not negatively affect the therapeutic efficacy of L-DOPA in the PD model, as this has been an issue with serotonin agonist-type therapies in clinical trials for LID [Cheshire 2012, Kannari 2002, Olanow 2004], and an imperative problem to mitigate for all future therapies. The current studies demonstrate that this gene therapy approach of providing DA autoregulatory properties to DRN neurons results in no changes in motor improvement between control and D2Rs animals. This was confirmed in two separate cohorts of rats and using two different motor tests. Both tests demonstrated that rats with the D2Rs in DRN neurons maintain a significant improvement in motor function with the administration of L-DOPA, reflecting recovery back to a pre-lesion state. This shows that D2Rs activity in serotonergic terminals of the striatum (or elsewhere) does not interfere with the pharmacological benefits of L-DOPA, and implicates D2Rs therapy as a potential potent treatment option for LID. It is important to note that while many preclinical studies using 5-HT agonists did not show an effect on L-DOPA-induced motor improvement, these results have not translated clinically. While multiple trials have used a variety of 5-HT agonists and seen reductions in AIM scores, many of these compounds contribute to worsening of parkinsonian symptoms and OFF L-DOPA periods, or have been abandoned due to lack of antidyskinetic efficacy (reviewed in [Cheshire 2012]). The discrepancy between the D2Rs approach and the use of agonists is unclear given that these two approaches conceivably evoke the same mechanism. Nevertheless, 5-HT1 compounds may produce their own side effects [Lindenbach 2015]. Second, their effects are dependent on an exogenously administered compound and hold a potential for suboptimal dosing (and timing of administration) as opposed to a gene therapy approach. Nevertheless, further studies are warranted to determine if D2Rs expression in the raphe is successful in other preclinical models of LID.
[0199] Pharmacological manipulations of 5-HT neurons in the treatment of LID, although successful pre-clinically, have not been fully translated. The transient nature of the anti-dyskinetic effect of currently available 5-HT approaches may be due to pharmacologic limitations of these drugs, including lack of specificity and potency for the specific receptor. Moreover, timing and comparative pharmacodynamics with L-DOPA delivery may be preventative [Mazzucchi 2015]. Because of this, a genetic approach in the form of continuous 5-HT inhibition should bypass such pharmacological limitations and provide meaningful and lasting protection against LID. Moreover, the finding that D2Rs gene therapy does not interfere with L-DOPA efficacy in the rat model provides promise for such an approach. Of course, the DR innervates a large part of the brain, providing many crucial functions, and the D2Rs therapy undertaken here does not distinguish between various projections. Thus, understanding any off-target effects from DA-mediated regulation of 5-HT neurons remains one important caveat that requires further research. The study was limited, and provided no genetic precision with the vector delivery as would be afforded in, for instance, a CRE animal. Although a majority of somatic transduction was observed in the area of the DR, it is also possible that other circuits were transduced with the vectors.
[0200] Changes in 5-HT innervation occur concomitant with nigrostriatal denervation and PD. Both 5-HT hyperinnervation [Bedard 2011, Politis 2010, Rylander 2010] as well as a decrease in 5-HT terminals [Guttman 2007, Kim 2003, Kish 2008, Scatton 1983] has been documented in human disease. Although the cause of these divergent findings is unknown, it is highly likely that 5-HT neurons play an important role in PD symptomology and, as the findings would suggest, in LID. As nigrostriatal denervation in human PD is near complete at the time of diagnosis [Kordower 2013] it is conceivable to speculate that changes in 5-HT innervation and function—and the capacity of these neurons to release DA—is a crucial component to dyskinesogenesis. To that end, understanding both the mechanisms of how 5-HT neurons process and release DA, and the underlying etiology of presynaptic 5-HT changes are important components to understand LID etiology and PD nonmotor symptoms, and represents a new therapeutic modality.
[0201] In conclusion, DA release from DRN 5-HT neurons can be regulated with ectopic expression of D2Rs, altering the activity and DA release properties of these neurons in a therapeutically meaningful way.
[0202]
[0203]
[0204]
[0205]
[0206]
[0207]
[0208]
[0209]
[0210]
[0211] As used herein, the term “combinations” shall be taken to mean one or more substances which can be administered together, one after the other or separately in one combined unit dosage form or in two separate unit dosage forms.
[0212] Administration of the dosage forms may be co-cominantly, simultaneously, part-simultaneously, separately or sequentially. The dosage forms of the combination may not necessarily be of the same dosage form and may comprise one or more of: Enteral: Oral (capsule, tablet, solution), Rectal (suppository) Parenteral: Intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, intramammary injection Respiratory: Inhalation, Intranasal, Intratracheal Topical: Mucous membrane application, skin application.
[0213] In addition, the release profiles of the medicaments may not be the same, for example one or more component of the combination may be of extended release form.
[0214] Compounds having mGluR modulating activity, in particular antagonistic activity, may be used to treat Parkinson's Disease and disorders associated with Parkinson's Disease. See, U.S. Pat. No. 8,703,809. In particular, mGluR modulators may be used to treat dyskinesia, a disorder associated with Parkinson's Disease and treatment thereof. In particular, it has been found that mGluR5 modulators, e.g. mGluR5 antagonists, may be used to treat Parkinson's Disease and associated disorders e.g. LID.
[0215] For the above-mentioned indications (the conditions and disorders) the appropriate dosage(s) will vary depending upon, for example, the compound(s) employed, the host, the mode of administration and the nature and severity of the condition being treated. For compounds disclose in the prior art literature, the recommendations and regulatory agency approved package inserts provide reasonable guidelines for predictable administration of the respective agents, though due care should be exercised for combinations of agents that may act additively, synergistically, or antagonistically at the same receptors, or in their functional results.
[0216] For use according to the invention, the various agents and compositions may be administered as single active agent or in combination with other active agents, in any usual manner, e.g. orally, for example in the form of tablets or capsules, or parenterally, for example in the form of injection solutions or suspensions. Moreover, these may be in association with at least one pharmaceutical carrier or diluent for use in the treatment of, e.g., Parkinson's Disease or LID. Such compositions may be manufactured in conventional manner.
[0217] The pharmaceutical compositions according to the invention are compositions for enteral, such as nasal, rectal or oral, or parenteral, such as intramuscular or intravenous, administration to warm-blooded animals (human beings and animals) that comprise an effective dose of the pharmacological active ingredient alone or together with a significant amount of a pharmaceutically acceptable carrier. The dose of the active ingredient depends on the species of warm-blooded animal, body weight, age and individual condition, individual pharmacokinetic data, the disease to be treated and the mode of administration. The pharmaceutical compositions comprise from approximately 1% to approximately 95%, preferably from approximately 20% to approximately 90%, active ingredient Pharmaceutical compositions according to the invention may be, for example, in unit dose form, such as in the form of ampoules, vials, suppositories, dragees, tablets or capsules. The pharmaceutical compositions of the present invention are prepared in a manner known per se, for example by means of conventional dissolving, lyophilizing, mixing, granulating or confectioning processes. Such processes are exemplified in WO 2005/079802, WO 2003/047581, WO 2004/000316, WO 2005/044265, WO 2005/044266, WO 2005/044267, WO 2006/114262 and WO 2007/071358.
[0218] Pharmaceutical compositions and medicaments may be described as mixtures of two or more components “by volume,” which is herein defined as the volume due to one component divided by the volume of all components of the composition. This ratio may be converted to or reported as a percentage of the total composition volume. Such a quantity may also be indicated by “v/v” or “percent v/v.” Similarly, the phrases “by weight” and “by mass” describe the weight or mass due to one component divided by the weight or mass of all components of the composition. This ratio may be converted to or reported as a percentage of the total composition weight or mass. Such a quantity may also be indicated by “w/w”, “mass percent” or percent w/w.”
[0219] A further aspect of the present invention is a kit for the prevention of, delay of progression of, treatment of a disease or condition according to the present invention comprising (a) an amount of a SERT-active agent having 5-HT1A receptor agonistic properties, or a pharmaceutically acceptable salt thereof, in a first unit dosage form; (b) an amount of at least one active ingredient selected from L-DOPA, and/or a DOPA decarboxylase inhibitor, or a catechol-O-methyl transferase inhibitor, or a dopamine agonist or, in each case, where appropriate, a pharmaceutically acceptable salt thereof; and (c) a container for containing said first, second etc. unit forms.
[0220] In a variation thereof, the present invention likewise relates to a “kit-of-parts”, for example, in the sense that the components to be combined according to the present invention can be dosed independently or by use of different fixed combinations with distinguished amounts of the components, i.e. simultaneously or at different time points.
[0221] The parts of the kit of parts can then e.g. be administered simultaneously or chronologically staggered, that is at different time points and with equal or different time intervals for any part of the kit of parts. Preferably, the time intervals are chosen such that the effect on the treated disease or condition in the combined use of the parts is larger than the effect that would be obtained by use of only any one of the components.
[0222] The present invention thus also relates to a kit of parts comprising (a) an amount of the agent or a pharmaceutically acceptable salt thereof in a first unit dosage form; (b) an amount of at least one active ingredient selected from L-DOPA, and/or a DOPA decarboxylase inhibitor, or a catechol-O-methyl transferase inhibitor, or a dopamine agonist or, in each case, where appropriate, a pharmaceutically acceptable salt thereof, in the form of two or three or more separate units of the components (a) to (b), especially for the prevention of, delay of progression of, treatment of a disease or condition according to the present invention.
[0223] The invention furthermore relates to a commercial package comprising the combination according to the present invention together with instructions for simultaneous, separate or sequential use.
[0224] In a preferred embodiment, the (commercial) product is a commercial package comprising as active ingredients the combination according to the present invention (in the form of two or three or more separate units of the components (a) or (b)), together with instructions for its simultaneous, separate or sequential use, or any combination thereof, in the delay of progression or treatment of the diseases as mentioned herein.
[0225] All the preferences mentioned herein apply to the combination, composition, use, method of treatment, “kit of parts” and commercial package of the invention.
[0226] These pharmaceutical preparations are for enteral, such as oral, and also rectal or parenteral, administration to homeotherms, with the preparations comprising the pharmacological active compound either alone or together with customary pharmaceutical auxiliary substances. For example, the pharmaceutical preparations consist of from about 0.1% to 90%, preferably of from about 1% to about 80%, of the active compound. Pharmaceutical preparations for enteral or parenteral, and also for ocular, administration are, for example, in unit dose forms, such as coated tablets, tablets, capsules or suppositories and also ampoules. These are prepared in a manner that is known per se, for example using conventional mixing, granulation, coating, solubilizing or lyophilizing processes. Thus, pharmaceutical preparations for oral use can be obtained by combining the active compound(s) with solid excipients, if desired granulating a mixture which has been obtained, and, if required or necessary, processing the mixture or granulate into tablets or coated tablet cores after having added suitable auxiliary substances. The dosage of the active compound can depend on a variety of factors, such as mode of administration, homeothermic species, age and/or individual condition. Preferred dosages for the active ingredients of the pharmaceutical combination according to the present invention are therapeutically effective dosages, especially those which are commercially available. The dosage of the active compound can depend on a variety of factors, such as mode of administration, homeothermic species, age and/or individual condition.
[0227] The pharmaceutical composition according to the present invention as described hereinbefore may be used for simultaneous use or sequential use in any order, for separate use or as a fixed combination.
[0228] One skilled in the art will readily appreciate that the specific methods and results discussed are merely illustrative of the invention as described more fully in the claims which follow thereafter.
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