TEXTILE TREATMENT COMPOSITIONS

Abstract

A novel liquid composition that provides antimicrobial and antiviral disinfection containing a quaternary ammonium salt fabric softener and a quaternary silicon compound which can be applied in a washing machine during the rinse cycle. The mixture is efficacious against most bacteria, virus and micro-organism. The dispensing in the wash cycle and machine drying sets the mixture into the fabric for residual efficacy. The compounds provide for softening and deodorizing of textiles garments. In addition, the compounds provide for sanitizing and extended residual sanitized efficacy for textiles garments. The compounds provide efficacy against many common virus, bacteria, including MRSA.

Claims

1. A liquid antimicrobial and antiviral fabric treating composition comprising: (a) a quaternary ammonium compound having fabric softening properties; and (b) an antimicrobial agent having the formula I ##STR00006## wherein R.sub.1 is an C.sub.1-30 alkyl group, preferably C.sub.8-30 alkyl group, R.sub.2 and R.sub.3, R.sub.4 and R.sub.5 each independently are an C.sub.1-30 alkyl group or hydrogen, and X is a counter ion, such as Cl.sup.−, Br.sup.−, I.sup.− or CH3COO.sup.−.

2. The liquid composition of claim 1, wherein the quaternary ammonium fabric softener is a compound of the formula II: ##STR00007## wherein R.sub.4 is hydrogen or lower alkyl of 1 to 4 and preferably 1 to 3 and especially preferably 1 or 2 carbon atoms, R.sub.5 is an alkyl containing from 9 to 25 carbon atoms, preferably linear higher alkyl of from about 13 to 23 and especially preferably 13 to 19 carbon atoms, R.sub.6 is an alkyl containing from 8 to 25 carbons and preferably a substantially linear higher alkyl group of about 13 to 23, and preferably 13 to 19 carbon atoms; R.sub.7 is hydrogen or an alkyl containing from 1 to 4 carbon atoms, preferably 1 or 2 carbon atoms, and X is as defined above.

3. The liquid composition of claim 2, wherein said quaternary ammonium fabric softener is selected from the group consisting of: methyl-1-tallow amido-ethyl-2-tallow imidazolinium methyl sulfate, methyl-1-oleyl amido-ethyl-2-oleyl imidazolinium methyl sulfate, 1-methyl-1-(palmitoylamido)-ethyl-2-octadecyl-4,5-dihydroimidazolinium chloride, 2-hepadecyl-1-methyl-1-(2-stearylamido)-ethyl-imidazolinium chloride, 2-lauryl-1-hydroxyethyl-1-oleyl-imidazolinium chloride, 2-tallow-1-(2-stearoyloxyethyl)-imidazoline chloride, 2-tallow-1-(2-stearoyloxyethyl)-imidazoline sulfate, 2-tallow-1-(2-stearoyloxyethyl)-imidazoline methosulfate, 2-tallow-1-methyl-3-(2-stearoylamidoethyl)-imidazoline chloride, 2-tallow-1-methyl-3-(2-stearoylamidoethyl)-imidazoline sulfate, 2-tallow-1-methyl-3-(2-stearoylamidoethyl)-imidazoline methosulfate, 2-heptadecyl-1-methyl-1-oleylamidoethyl-imidazolinium-metho-sulfate, 2-heptadecyl-1-methyl-1-(2-stearoylamido)ethyl-imidazolinium-sulfate, 2-coco-1-(2-hydroxyethyl)-1-benzyl-imidazolinium-chloride, 2-coco-1-(2-hydroxyethyl)-1-(4-chlorobutyl)-imidazolinium-chloride, 2-coco-1-(2-hydroxyethyl)-1-octadecenyl-imidazolinium-chloride, 2-tallow-1-(2-hydroxyethyl)-1-benzyl-imidazolinium-chloride, 2-tallow-1-(2-hydroxyethyl)-1-(4-chlorobutyl)-imidazolinium-chloride, 2-heptadecenyl-1-(2-hydroxyethyl)-1-(4-chlorobutyl)-imidazolinium-chloride, 2-heptadecenyl-1-(2-hydroxyethyl)-1-benzyl-imidazolinium-chloride and 2-heptadecenyl-1-(2-hydroxyethyl)-1-octadecyl-imidazolinium-chloride.

4. The liquid composition of claim 1, wherein the quaternary ammonium fabric softener is a compound of the formula HI: ##STR00008## wherein R represents a hydrocarbyl group of from about 12 to 24 and preferably about 14 to 22 carbon atoms; R.sub.1 represents lower alkyl of 1 to 4, preferably 1 to 3, carbon atoms, or a hydrocarbyl group of from 12 to 24, preferably 14 to 22, carbon atoms; R.sub.2 and R.sub.3 represent lower alkyl of 1 to 4, preferably 1 to 3, carbon atoms, and X represents an anion capable of imparting water solubility or dispersibility, such as halide, e.g. chloride, bromide and iodide; sulfate, methosulfate, nitrite, nitrate, phosphate, and carboxylate, e.g. acetate, adipate, propionate, phthalate, benzoate and oleate.

5. The liquid composition of claim 4, wherein said quaternary ammonium fabric softener is selected from the group consisting of: distearyl dimethyl ammonium chloride, ditallow dimethyl ammonium chloride, dihexadecyl dimethyl ammonium chloride, distearyl dimethyl ammonium bromide, di(hydrogenated tallow) dimethyl ammonium bromide, distearyl di(isopropyl)ammonium chloride, distearyl dimethyl ammonium methosulfate and dilauryldimethylammonium chloride.

6. The liquid composition of claim 1, wherein the antimicrobial agent of formula I is selected from the group consisting of: 3-(triethoxysilyl)-propyl-dimethyloctadecyl ammonium chloride, 3-(trimethoxysilyl)-propyl-methyl-dioctyl ammonium chloride, 3-(trimethoxysilyl)-propyl-dimethyldecyl ammonium chloride, 3-(trimethoxysilyl)-propyl-methyldidecyl ammonium chloride, 3-(trimethoxysilyl)-propyl-dimethyldodecyl ammonium chloride, 3-(trimethoxysilyl)-propyl-methyldidodecyl ammonium chloride, 3-(trimethoxysilyl)-propyl-dimethyltetradecyl ammonium chloride, 3-(trimethoxysilyl)-propyl-methyldihexadecyl ammonium chloride, and 3-(trimethoxysilyl)-propyl-dimethyloctadecyl ammonium chloride.

7. The liquid composition of claim 1, wherein said composition can be applied on garments in textile mills or in the home washing machine, or rinse cycle.

8. The liquid composition of claim 1, wherein said composition provides a softened hand feel on the textile garment.

9. The liquid composition of claim 1, wherein said composition removes and reduces odors on the textile garment.

10. The liquid composition of claim 1, wherein said composition provides the efficacy of removal or reduction in microorganism population on the garment.

11. The liquid composition of claim 1, wherein said composition once infused into the garments fibers provides persistent efficacy as an antimicrobial and anti-viral.

12. The liquid composition of claim 1, wherein said composition is efficacious against E coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae and Methicillin resistant Staphylococcus aureus, Influenza, Coronaviridae and Noro virus families

13. The liquid composition of claim 1, wherein said composition is non-allergenic on garments and clothing.

14. The liquid composition of claim 1, wherein said composition is applied on textile substrates selected from the group consisting of cotton, polyester blends, and polyether-polyurea copolymers such as spandex, lycra or elastane.

15. The liquid composition of claim 1, wherein said composition provides antistatic properties to the textile garment.

16. The liquid composition of claim 1, wherein said composition provides hospital linens and uniforms with antimicrobial properties to protect the hospital care worker.

17. The liquid composition of claim 1, further including polyhexylmethylene biguanide HCl.

18. The liquid composition of claim 1, wherein said composition further includes a nonionic surfactant.

19. The liquid composition of claim 1, wherein said composition further includes a preservative.

20. The liquid composition of claim 1, wherein said composition when applied to surgical masks prevents microorganisms from traveling through the textile filter due to cationic charge and van der Walls attraction.

21. The liquid composition of claim 1, wherein said composition can be applied to treat medical bandages, gauze, pre-op non-woven, cotton gloves and surgical disposable textiles and cellulosic paper barriers.

22. A method for providing antimicrobial and antiviral properties to a fabric, said method comprising contacting said fabric with the composition of claim 1.

Description

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0042] The term “antimicrobial”, as used herein, refers to the capability of an article to destroy (kill), inhibit the growth or reproduction of, immobilize and/or remove microorganisms, for example bacteria, fungi, yeasts and algae.

[0043] The term “antiviral” as used herein, refers to the capability of an article to destroy (kill), inhibit the growth or reproduction of, immobilize and/or remove viruses.

[0044] The compositions of the present invention provide anti-microbial and antiviral protection to fabrics. The also provide anti-viral protection against corona-viruses and more in particular Covid 19 also known as SARS-2.

[0045] The compositions of the present invention are stable aqueous compositions which contain as the active fabric softening components cationic fabric softeners or mixtures thereof which is preferably a water-dispersible quaternary ammonium compound salt or a water dispersible alkyl imidazolinium compound salt or a water dispersible alkyl imidazolinium compound salt as hereinafter described in more detail, and or other ingredients such as fatty alcohols, nonionic ethoxylated or propoxylated alcohols also as hereinafter described.

[0046] The aqueous compositions of this invention contain as the first active fabric softening component at least one cationic fabric softener in an amount generally ranging from about 74 to 86% by weight, preferably from about 75 to 85% by weight of the total active fabric softening components (cationic fabric softener plus other ingredients), or on the basis of the total aqueous composition, the amount will be: for a concentrated formulation, from about 8 to 15% by weight, preferably from about 10 to 13%, by weight and for a ready-to-use formulation from about 2.2 to 7%, preferably from about 2.3 to 6.6%, by weight.

[0047] It is understood, of course, that a ready-to-use product is one for which the consumer is instructed to use the product at full strength (i.e. without further dilution except, of course, for the rinse water, etc.) at the specified dosage level, e.g. ¼ to ½ cup per load of wash (usually about 6 to 15 pounds). On the other hand, a concentrated formula is one for which the consumer is instructed to dilute the product (e.g. from about 1 to 4×) to make up the required dosage, e.g. ¼ to ½ cup per load of wash. Accordingly, it is clear that for the preferred concentrated formula, the consumer will have at least the perceived notion of greater economy and flexibility in use.

[0048] Softening agents are used to render fabrics or textile soft, and the terms “softening” and “softener” refer to the handle, hand, touch, or feel; this is the tactile impression given by fabrics or textiles to the hand or body and is of aesthetic and commercial importance. The cationic fabric softeners used in the present invention can be any of the commercially available and known cationic fabric softeners and preferably are of the water-dispersible quaternary ammonium compound salt or alkyl imidazolinium compound salt type including at least one, and preferably two hydrophobic groups containing at least 12 and preferably at least 14 carbon atoms.

[0049] A first preferred class of the cationic fabric softener active ingredient are the imidazolinium compounds of the formula I:

##STR00002##

wherein R.sub.4 is hydrogen or lower alkyl of 1 to 4 and preferably 1 to 3 and especially preferably 1 or 2 carbon atoms, R.sub.5 is an alkyl containing from 9 to 25 carbon atoms, preferably linear higher alkyl of from about 13 to 23 and especially preferably 13 to 19 carbon atoms, R.sub.6 is an alkyl containing from 8 to 25 carbons and preferably a substantially linear higher alkyl group of about 13 to 23, and preferably 13 to 19 carbon atoms; R.sub.7 is hydrogen or an alkyl containing from 1 to 4 carbon atoms, preferably 1 or 2 carbon atoms, and X is as defined above.

[0050] Typical examples of the alkyl imidazolinium compounds of formula I include: [0051] methyl-1-tallow amido-ethyl-2-tallow imidazolinium methyl sulfate, [0052] methyl-1-oleyl amido-ethyl-2-oleyl imidazolinium methyl sulfate, [0053] 1-methyl-1-(palmitoylamido)-ethyl-2-octadecyl-4,5-dihydroimidazolinium chloride, [0054] 2-hepadecyl-1-methyl-1-(2-stearylamido)-ethyl-imidazolinium chloride, [0055] 2-lauryl-1-hydroxyethyl-1-oleyl-imidazolinium chloride, [0056] 2-tallow-1-(2-stearoyloxyethyl)-imidazoline chloride, [0057] 2-tallow-1-(2-stearoyloxyethyl)-imidazoline sulfate, [0058] 2-tallow-1-(2-stearoyloxyethyl)-imidazoline methosulfate, [0059] 2-tallow-1-methyl-3-(2-stearoylamidoethyl)-imidazoline chloride, [0060] 2-tallow-1-methyl-3-(2-stearoylamidoethyl)-imidazoline sulfate, [0061] 2-tallow-1-methyl-3-(2-stearoylamidoethyl)-imidazoline methosulfate, [0062] 2-heptadecyl-1-methyl-1-oleylamidoethyl-imidazolinium-metho-sulfate, [0063] 2-heptadecyl-1-methyl-1-(2-stearoylamido)ethyl-imidazolinium-sulfate, [0064] 2-coco-1-(2-hydroxyethyl)-1-benzyl-imidazolinium-chloride, [0065] 2-coco-1-(2-hydroxyethyl)-1-(4-chlorobutyl)-imidazolinium-chloride, [0066] 2-coco-1-(2-hydroxyethyl)-1-octadecenyl-imidazolinium-chloride, [0067] 2-tallow-1-(2-hydroxyethyl)-1-benzyl-imidazolinium-chloride, [0068] 2-tallow-1-(2-hydroxyethyl)-1-(4-chlorobutyl)-imidazolinium-chloride, [0069] 2-heptadecenyl-1-(2-hydroxyethyl)-1-(4-chlorobutyl)-imidazolinium-chloride, [0070] 2-heptadecenyl-1-(2-hydroxyethyl)-1-benzyl-imidazolinium-chloride and [0071] 2-heptadecenyl-1-(2-hydroxyethyl)-1-octadecyl-imidazolinium-chloride.

[0072] Another preferred class of the cationic softeners are the quaternary ammonium salts of the formula II:

##STR00003##

wherein R represents a hydrocarbyl group of from about 12 to 24 and preferably about 14 to 22 carbon atoms; R.sub.1 represents lower alkyl of 1 to 4, preferably 1 to 3, carbon atoms, or a hydrocarbyl group of from 12 to 24, preferably 14 to 22, carbon atoms; R.sub.2 and R.sub.3 represent lower alkyl of 1 to 4, preferably 1 to 3, carbon atoms, and X represents an anion capable of imparting water solubility or dispersibility, such as halide, e.g. chloride, bromide and iodide; sulfate, methosulfate, nitrite, nitrate, phosphate, and carboxylate, e.g. acetate, adipate, propionate, phthalate, benzoate, oleate, etc.).

[0073] The hydrocarbyl groups are preferably alkyl but may be alkenyl, aryl, or aralkyl and may include various substituents or interrupting groups such as halo, amide, hydroxyl, and carboxyl substituents or interrupting functional groups and ethoxy or polyethoxy interrupting groups. In addition, one or more of the lower alkyl groups may also be substituted, for example, by an hydroxy group. Typical cationic fabric softener compounds of formula III include the following: distearyl dimethyl ammonium chloride, ditallow dimethyl ammonium chloride, dihexadecyl dimethyl ammonium chloride, distearyl dimethyl ammonium bromide, di(hydrogenated tallow) dimethyl ammonium bromide, distearyl di(isopropyl)ammonium chloride, distearyl dimethyl ammonium methosulfate and dilauryldimethylammonium chloride.

[0074] A highly preferred class of the cationic fabric softeners of formula HI are the water-insoluble compounds wherein the groups R and R.sub.1 are C.sub.14 to C.sub.18, R.sub.2 is methyl or ethyl and R.sub.3 is methyl, ethyl, isopropyl, n-propyl, hydroxyethyl or hydroxypropyl.

[0075] The water dispersible cationic fabric softeners which can be used in the compositions of the present invention are not limited to those described above and any of the other known useful water dispersible cationic fabric softeners can be used. Furthermore, mixtures of the above mentioned cationic fabric softeners can also be used.

[0076] The anti-microbial and antiviral component of the formulation is a suitable organosilicon quaternary ammonium salt compound represented by Formula F

##STR00004##

wherein R.sub.1 is an C.sub.1-30 alkyl group, preferably C.sub.8-30 alkyl group, R.sub.2 and R.sub.3, R.sub.4 and R.sub.5 each independently are an C.sub.1-30 alkyl group or hydrogen, and X is a counter ion, such as Cl.sup.−, Br.sup.−, I.sup.− or CH3COO.sup.−.

[0077] A preferred organosilicon quaternary ammonium salt has the structural formula

##STR00005##

[0078] Other examples of organosilicon quaternary ammonium salt compounds for use according to the invention are 3-(triethoxysilyl)-propyl-dimethyloctadecyl ammonium chloride, 3-(tri-methoxysilyl) propyl-methyl-dioctyl ammonium chloride, 3-(trimethoxysilyl) propyl-dimethyl-decyl ammonium chloride, 3-(trimethoxysilyl)-propyl-methyldidecyl ammonium chloride, 3-(trimethoxysilyl) propyldimethyldodecyl ammonium chloride, 3-(tri-methoxysilyl)-propyl-methyl didodecyl ammonium chloride, 3-(trimethoxysilyl) propyl-dimethyltetradecyl ammonium chloride, 3-(trimethoxysilyl)-propyl-methyldihexadecyl ammonium chloride, and 3-(trimethoxy-silyl) propyl-dimethyloctadecyl ammonium chloride.

[0079] More preferably, the organosilicon quaternary ammonium salt compound is a 3-(tri-methoxysilyl) propyl-dimethyloctadecyl ammonium halide, most preferably 3-(trimethoxysilyl) propyl-dimethyloctadecyl ammonium chloride (R.sub.1=—C.sub.18H.sub.37, R.sub.2=—CH.sub.3, R.sub.3=—CH.sub.3, R.sub.4=—C.sub.3H.sub.6, R.sub.5=—CH.sub.3, and X=Cl.sup.−.

[0080] The concentration of the organosilicon quaternary is in the range of 1-5% by weight and more preferably in the range of 1-2.5% by weight.

[0081] Aqueous or alcoholic solutions of the compositions of the invention can be used to treat textiles, minerals, glass and many other substrates, to render the surfaces of the substrates resistant to the growth of microorganisms or viruses. Preferably, the textile fabrics treated with the compositions of the invention have free hydroxy groups on their surfaces. In this regard, it has been found that, after treatment of a substrate surface, having free hydroxy groups, with an alcohol solution of the compositions, the composition is not readily washed off or leached from the treated substrate surface by water. It is believed that this phenomenon results from the formation of covalent bonds between the silyl moiety of the silicon quaternary and the free hydroxy groups on the surface of the substrate, whereby the composition is chemically bonded to the substrate's surface.

[0082] Any substrate having free hydroxy groups on its surface can be usefully treated with the composition of the invention. Among the substrates having free hydroxy groups on their surfaces, which can be suitably bonded to the organosilicon quaternary ammonium compounds, are natural and man-made fiber fabrics, such as cotton, wool, linen, felt, acrylic, polyester, cellulose acetate, rayon, nylon and vinyl, and metals such as aluminum, stainless steel and galvanized metal, as well as wood, rubber, paper, glass and ceramic.

[0083] In the presence of moisture, this antimicrobial silicon containing quaternary ammonium agent imparts a durable, wash resistant, broad spectrum biostatic surface antimicrobial and antiviral finish to a substrate. The organosilicon quaternary ammonium compound is leach resistant, nonmigrating and is not consumed by microorganisms. It is effective against gram positive and gram negative bacteria, viruses, fungi algae, yeasts, mold, rot and mildew. The silicone quaternary ammonium salt provides durable, bacteriostatic, virucidal, fungistatic and algistatic surfaces. It can be applied to organic or inorganic surfaces as a dilute aqueous or solvent solution of 0.1-1.5 percent by weight of active ingredient. After the alkoxysilane is applied to a surface, it is chemically bonded to the substrate by condensation of the silanol groups at the surface. The pure compound is crystalline whereas methanol solutions of the compound are low viscosity, light to dark amber liquids, soluble in water, alcohols, ketones, esters, hydrocarbons and chlorinated hydrocarbons. The compound has been used in applications such as, for example, socks, filtration media, bed sheets, blankets, bedspreads, carpet, draperies, fire hose fabric materials, humidifier belts, mattress pads, health care apparel, mattress ticking, underwear, nonwoven disposable diapers, nonwoven fabrics, outerwear fabrics, nylon hosiery, vinyl paper, wallpaper, polyurethane cushions, roofing materials, sand bags, tents, tarpaulins, sails, rope, blood pressure cuffs, athletic and casual shoes, shoe insoles, shower curtains, toilet tanks, toilet seat covers, throw rugs, towels, umbrellas, upholstery fiberfill, intimate apparel, wiping cloths and medical devices such as blood pressure cuffs.

[0084] The compositions of the invention are prepared by dissolving the components in water. The resulting aqueous solutions may also include nonionic surfactants, phenoxyethanol and fragrances.

[0085] The compositions may also contain one or more optional additives selected from the group consisting of perfumes, dyes, pigments, opacifiers, optical brighteners, anti-corrosion agents, and preservatives, the amount of each additive being up to 0.5% by weight, and

[0086] In further embodiments of the invention, the starting textile material can be treated with one or more further antimicrobial agents, in particular at least one selected from the group consisting of benzalkonium chloride; benzethonium chloride; benzoxonium chloride; dequa-linium; vinylbenzyltrimethylammonium chloride; cetrimonium bromide, optionally in combination with reactive amino silicone having alkoxy groups like hydroxyl or methoxy or ethoxy groups; 2-phenylphenol, Acibenzolar, Paclobutrazol, Azoxystrobin, Epoxiconazole, Binapacryl, Iprodion, Triadimefon, Fuberidazole, Flusilazole, 2,4,6-tribromophenol, Vinciozolin, Pyrazophos, Tebuconazole, Metalaxy, Dichlofluanid, Strobilurins, Myclobutanil, Fenpropimorph with blocked isocyanate, vinylbenzyltrimethylammonium chloride, didecyldimethylammonium chloride, Fenticlor, 9-aminoacridine, polyhexamethylene biguanide, Dibromopropamidine, Chlorothalonil, Povidone-Iodine, Fenamidone, Pencycuron, cetyl pyridinium chloride, Cetrimonium, cetyl Trimethylammonium, Bupirimate, Fluopicolide, Hexachlorophene, Triclo-carban, Nitrofuran, Clioquinol, methyl-paraben, Propamocarb, cinnamaldehyde, hexamidine, and Falcarindio. The further antimicrobial agent is preferably used in an amount of 0.1 to 10% by weight, in particular in an amount of 0.1 to 5%, 0.1 to 3% or 0.1 to 1% by weight, based on the weight of the textile material.

[0087] Nonionic materials may also be incorporated in the compositions of the invention. Typical non-ionics include alkylphenoxypolyethoxyethanols having alkyl groups of about seven to eighteen carbon atoms and 6 to 60 or more oxyethylene units, such as heptyl-phenoxypolyethoxyethanols, octylphenoxynonylphenoxyethanols, dodecylphenoxypolyethoxy-ethanols, and the like; and polyethoxy ethanol derivatives of methylene-linked alkyl phenols.

[0088] The invention incorporates cationic compounds in solution to perform several functions.

Example I

[0089] In the first set of formulations, two distinct chemistries were combined namely a cationic softening agent, 4,5 Dihydro-1-methyl-2-nortallow alkyl-1-(2-tallow amidoethyl) imidazolinium compound methyl sulfates; and a cationic biocide namely, l-Octadecanaminium-N,N-Dimethyl-N-[(3-trimethoxysilyl) propyl] chloride.

[0090] Imidazolinium DMS quaternaries are considered the best softeners on an activity basis providing exceptional softening properties in addition to providing superior static control. 4,5 Dihydro-1-methyl-2-nortallow alkyl-1-(2-tallow amidoethyl) imidazolinium compound methyl sulfates are noted for a low base odor.

[0091] The 1-Octadecanaminium-N,N-Dimethyl-N-[(3-trimethoxysilyl) propyl] chloride has a known reliability to reduce micro-organisms while binding to the textile fabric. This compound has been used on textiles and hosiery for over 40 years.

[0092] Further formulations used other biocides in addition to l-Octadecanaminium-N,N-Dimethyl-N-[(3-trimethoxysilyl) propyl] chloride to enhance the antimicrobial efficacy. The efficacy was enhanced by the addition of ammonium chloride quaternaries and polyhexa-methylene diguanide HCl.

[0093] In experimental trials 1 through 5, the compounds were mixed in a glass reactor at 50 C into deionized water.

TABLE-US-00002 TABLE 1 Formulations For Antimicrobial Softener TRIAL TRIAL TRIAL TRIAL TRIAL ITEM RAW MATERIAL 1 2 3 4 5 1 Methyl-1-Tallow Amidoethyl-2-  12.5%  12.5%  12.5%  6.2%  3.1% Tallow Imidazolinium Methyl Sulfates 2 1-Octadecaniminium-N,N-Dimethyl-  1.04%  1.04%  2.2%  2.2%  2.2% N-[(3trimethoxysilyl) Propyl] Chloride 3 Deionized Water 85.96% 85.46%  84.3%  90.6%  93.7% 4 Polyethoxylene Isodecyl Ether —  0.5%  0.5%  0.5%  0.5% 5 Phenoxyethanol  0.5%  0.5%  0.5%  0.5%  0.5% 6 Lemon Fragrance — — — — — Total   100%   100%   100%   100%   100%
Samples of the compounds were chosen to be tested at various laboratories for antimicrobial efficacy.

[0094] The compounds were designated to the laboratories with a trade name as “Laundry Complete”. In Formulation Trial 1 a 12.5% wt of 4,5 dihydro-1-methyl-2-nortallow alkyl-1(2-tallow amidoethyl) imidazolium compounds methyl sulfates (80% wt active) fabric softener was combined with a 1.039% wt of 1-octadecaminium-N,N-dimethyl-n-[(3-tri-methoxysilyl) propyl]chloride, (72% wt active) antimicrobial in demineralized water. The raw materials were mixed at 50 C and a macro emulsion was formed.

The formulation Trial 1 was subject to microbial testing against E coli and Staphylococcus aureus. Results yielded a log 0.44 kill for E coli Table 2 and a log 0.27 kill for Staphylococcus aureus.

TABLE-US-00003 TABLE 2 Antimicrobial Efficacy Laundry Complete With E Coli With Formulation Trial 1 (Table 1) AVERAGE NO NO BACTEIA BACTERIA IN OF COLONIES IN THE TEST PERCENTAGE TEST SERIAL CONTROL IN CONTROL SAMPLE LOG KILL ORGANISM NO CFU/ML CFU/ML CFU/ML KILL % Echerichia Coli 1 3.0 × 10.sup.7 3.3 × 10.sup.7 1.1 × 10.sup.7 0.44 63.333 8099 Echerichia Coli 2 3.3 × 10.sup.7 3.3 × 10.sup.7 1.2 × 10.sup.7 0.44 63.636 8099 Echerichia Coli 3 3.5 × 10.sup.7 3.3 × 10.sup.7 1.2 × 10.sup.7 0.46 65.714 8099
Technical Standard for Disinfection (2002 Ministry of Health P.R. China)-2.1.1.7.4 Suspension quantitative germicidal test. Test time 10 minutes.
Technical Standard for Disinfection (2002 Ministry of Health P.R. China)-2.1.1.5.5 Quantitative determination of neutralizer suspension.

TABLE-US-00004 TABLE 2.2 Antimicrobial Efficacy Laundry Complete With Staphylococcus Aureus Trial 1 (Table 1) AVERAGE No. No. BACTERIA BACTERIA IN OF COLONIES IN THE TEST PERCENTAGE TEST SERIAL CONTROL IN CONTROL SAMPLE LOG KILL ORGANISM No. CFU/ML CFU/ML CFU/ML KILL % Staphylococcus 1 4.2 × 10.sup.7 4.2 × 10.sup.7 2.3 × 10.sup.7 0.26 45.238 Aureus ATCC 6538 Staphylococcus 2 3.9 × 10.sup.7 4.2 × 10.sup.7 2.0 × 10.sup.7 0.29 48.718 Aureus ATCC 6538 Staphylococcus 3 4.5 × 10.sup.7 4.2 × 10.sup.7 2.4 × 10.sup.7 0.27 46.667 Aureus ATCC 6538
Technical Standard for Disinfection (2002 Ministry of Health P.R. China)-2.1.1.7.4 Suspension quantitative germicidal test. Test time 10 minutes.
Technical Standard for Disinfection (2002 Ministry of Health P.R. China)-2.1.1.5.5 Quantitative determination of neutralizer suspension.

[0095] While the log kills of 0.44 and 0.27 proved the antimicrobial agent 1-octadecaminium-N,N-dimethyl-n-[(3-trimethoxysilyl) propyl]chloride at 1.0% wt had an effect, the effect was not at the log 3 range (99.9%) needed in 10 minutes.

Example II

[0096] Formulations Trials 6 through Trial 10 from Table 3 added a mixture of ammonium chloride quaternaries to include alkyl dimethyl benzyl ammonium chloride, octadecyl dimethyl ammonium chloride, dioctyldecyl dimethyl ammonium chloride and didecyl dimethyl ammonium chloride at a 0.36 wt % to the 12.5% wt of 4,5 dihydro-1-methyl-2-nortallow alkyl-1(2-tallow amidoethyl) imidazolium compounds methyl sulfates (80% wt active) fabric softener combined with a 1.039% wt of l-octadecaminium-N,N-dimethyl-n-[(3-trimethoxysilyl) propyl]chloride, (72% wt active) antimicrobial in demineralized water.

[0097] A quantitative analysis on cotton fabric test was performed JIS L-1902. The cotton fabric was treated with Laundry Complete and held 5 days prior to commencement of JIS L-1902. The addition of 100 ml of Formulation Trial 8 from Table 3NS FOR LAUNDRYT COMPLETE per 10 Liter of water (1.0% per weight of goods) was added to the rinse cycle in a washing machine. The fabric was dried and held in constant relative humidity until dry. JIS L-1902 began. Table 4.1 shows that the efficacy for Staphylococcus aureus was log 5.8 after 20 hour duration. Table 4.2 shows the efficacy for Klebsiella Pneumoniae was log 6.3. Table 4.3 shows the efficacy for E coli was log 4.9. Table 4.4 shows the efficacy for Pseudomonas aeruginosa was log 2.2. Table 4.5 shows the efficacy for MRSA was log 6.1.

TABLE-US-00005 TABLE 3 FORMULATIONS FOR LAUNDRY COMPLETE TRIAL 6 TRIAL 7 TRIAL 8 TRIAL 9 TRIAL 10 CHEMICAL NAME WT % WT % WT % WT % WT % 4,5 Dihydro-1-Methyl-2-Nortallow 12.5 12.5 12.5% 12.5 6.25 Alkyl-1(2-Tallow Amidoethyl) Imidazolium Compounds Methyl Sulfates (80% Active) Imidazolinium Compounds 2-(C17 And 0 0 0 0 0 C17 Unsaturated Alkyl)-1-[2-(C18 And C18 Unsaturated Amido) Ethyl]-4,5 Dihydro-1-Methyl Sulfates In Propylene Glycol 1-Octadecaminium-N,N-Dimethyl-N-[(3- 1.039 1.039 1.039 1.039 1.039 Trimethoxysilyl) Propyl]Chloride (72.0% Active) Alkyl Dimethyl Benzyl Ammonium 0 0.36 0.36 0.36 0.36 Chloride & Octyl Decyl Dimethyl Ammonium Chloride & Dioctyl Dimethyl Ammonium Chloride & Didecyl Dimethyl Ammonium Chloride Polihexanide (20% Active) 0.50 0.50 0.50 Deionized Water 86.081 85.764 85.624 85.324 Phenoxyethanol 0.3 0.3 Polyoxyethylene 9 Tridecyl Ether 0.38 0.33 0.33 0.33 0.33 Fragrance 0.007 0.007 0.007

TABLE-US-00006 TABLE 4.1 Quantatative Method For Antimicrobial Efficacy By Absorbtion Method JIS L 1902: 2008 For Staphyloccocus Aureus Attc 6538 For Laundry Complete Table 3 Formula Trial 8 DIFFERENCE OF DIFFERENCE OF EXTREMES EXTREMES ANTI- CONCEN- FOR 3 CONTROL FOR 3 CONTROL GROWTH GROWTH MICROBIAL TRATION SPECIEMENS SPECIEMENS VALUE OF F VALUE OF G ACTIVITY OF (LOG) (LOG) (F = LOGC.sub.t − (G = LOGT.sub.t − VALUE HRS INOCULUM (CONDITION <1 (CONDITION <2 LOGC.sub.0) LOGT.sub.0) (A = F − G) 2.1 × 10.sup.5 +2.5 −3.3 5.8 LGC.sub.t: +7.1 LGT.sub.t: +1.3 LGC.sub.0: +4.6 LGT.sub.0: +1.3  0 HR <0.1 0.0 20 HR <0.1 0.0

TABLE-US-00007 TABLE 4.2 Quantatative Method For Antimicrobial Efficacy By Absorbtion Method Jis L 1902: 2008 For Klebsiella Pneumoniae ATCC 4352 For Laundry Complete Table 1 Formula Trial 2 DIFFERENCE OF DIFFERENCE OF EXTREMES EXTREMES ANTI- CONCEN- FOR 3 CONTROL FOR 3 CONTROL GROWTH GROWTH MICROBIAL TRATION SPECIEMENS SPECIEMENS VALUE OF F VALUE OF G ACTIVITY OF (LOG) (LOG) (F = LOGC.sub.t − (G = LOGT.sub.t − VALUE HRS INOCULUM (CONDITION <1 (CONDITION <2 LOGC.sub.0) LOGT.sub.0) (A = F − G) 1.8 × 10.sup.5 +3.1 −3.2 6.3 LGC.sub.t: +7.6 LGT.sub.t: +1.3 LGC.sub.0: +4.5 LGT.sub.0: +1.3  0 HR 0.1 0.0 20 HR <0.1 0.0

TABLE-US-00008 TABLE 4.3 Quantatative Method For Antimicrobial Efficacy By Absorbtion Method Jis L 1902: 2008 For Escherichia Coli ATCC 8739 For Laundry Complete Table 3 Formula Trial 8 DIFFERENCE OF DIFFERENCE OF EXTREMES EXTREMES ANTI- CONCEN- FOR 3 CONTROL FOR 3 CONTROL GROWTH GROWTH MICROBIAL TRATION SPECIEMENS SPECIEMENS VALUE OF F VALUE OF G ACTIVITY OF (LOG) (LOG) (F = LOGC.sub.t − (G = LOGT.sub.t − VALUE HRS INOCULUM (CONDITION <1 (CONDITION <2 LOGC.sub.0) LOGT.sub.0) (A = F − G) 2.4 × 10.sup.5 +3.4 −1.5 4.9 LGC.sub.t: +8.0 LGT.sub.t: +3.1 LGC.sub.0: +4.6 LGT.sub.0: +4.1  0 HR <0.1 <0.1 20 HR <0.1 <0.1

TABLE-US-00009 TABLE 4.4 Quantatative Method For Antimicrobial Efficacy By Absorbtion Method Jis L 1902: 2008 For Pseudomonas Aeruginosa ATCC 9027 For Laundry Complete Table 3 Formula Trial 8 DIFFERENCE OF DIFFERENCE OF EXTREMES EXTREMES ANTI- CONCEN- FOR 3 CONTROL FOR 3 CONTROL GROWTH GROWTH MICROBIAL TRATION SPECIEMENS SPECIEMENS VALUE OF F VALUE OF G ACTIVITY OF (LOG) (LOG) (F = LOGC.sub.t − (G = LOGT.sub.t − VALUE HRS INOCULUM (CONDITION <1 (CONDITION <2 LOGC.sub.0) LOGT.sub.0) (A = F − G) 2.4 × 10.sup.5 +3.0 0.8 2.2 LGC.sub.t: +7.6 LGT.sub.t: +5.4 LGC.sub.0: +4.6 LGT.sub.0: +4.5  0 HR <0.1 <0.1 20 HR 0.1 0.1

TABLE-US-00010 TABLE 4.5 Quantatative Method For Antimicrobial Efficacy By Absorbtion Method Jis L 1902: 2008 For Methicillin Resistant Staphylococcus Aureus ATCC 33591 For Laundry Complete Table 3 Formula Trial 8 DIFFERENCE OF DIFFERENCE OF EXTREMES EXTREMES ANTI- CONCEN- FOR 3 CONTROL FOR 3 CONTROL GROWTH GROWTH MICROBIAL TRATION SPECIEMENS SPECIEMENS VALUE OF F VALUE OF G ACTIVITY OF (LOG) (LOG) (F = LOGC.sub.t − (G = LOGT.sub.t − VALUE HRS INOCULUM (CONDITION <1 (CONDITION <2 LOGC.sub.0) LOGT.sub.0) (A = F − G) 1.5 × 10.sup.5 +3.0 −3.1 6.1 LGC.sub.t: +7.4 LGT.sub.t: +1.3 LGC.sub.0: +4.4 LGT.sub.0: +2.3  0 HR 0.1 0.1 20 HR 0.1 0.0

Example III

[0098] In Formulations from Table 5 Trials 11 through 15, the use of a 80% Active 4,5 dihydro-1-methyl-2-nortallow alkyl-1(2-tallow amidoethyl) imidazolium compounds methyl sulfates was used at various concentrations. The compounds were mixed in 50° C. Demineralized water with and without surfactants.

[0099] Stable emulsions were made, and viscosities were suitable for the 7.5% by wt 4,5 dihydro-1-methyl-2-nortallow alkyl-1(2-tallow amidoethyl) imidazolium compounds methyl sulfates

TABLE-US-00011 TABLE 5 FORMULATIONS FOR LAUNDRY COMPLETE TRIAL TRIAL TRIAL TRIAL TRIAL 11 12 13 14 15 CHEMICAL NAME Wt % Wt % Wt % Wt % Wt % 4,5 Dihydro-1-Methyl-2-Nortallow Alkyl- 11.1 7.5 6.2 6.2 6.2 1(2-Tallow Amidoethyl) Imidazolium Compounds Methyl Sulfates (80% Active) Imidazolinium Compounds 2-(C.sub.17 And C.sub.17 0 0 0 0 0 Unsaturated Alkyl)-1-[2-(C.sub.18 And C.sub.18 Unsaturated Amido) Ethyl]-4,5 Dihydro-1- Methyl Sulfates In Propylene Glycol 1-Octadecaminium-N,N-Dimethyl-N-[(3- 1.039 1.039 1.039 1.039 1.039 Trimethoxysilyl) Propyl]Chloride (72% Wt Active) Alkyl Dimethyl Benzyl Ammonium 0.36 2.25 2.25 2.25 2.25 Chloride & Octyl Decyl Dimethyl Ammonium Chloride & Dioctyl Dimethyl Ammonium Chloride & Didecyl Dimethyl Ammonium Chloride Polihexanide 0.50 0.50 Deionized Water 86.864 90.464 91.864 92.194 91.371 Phenoxyethanol 0.3 0.3 0.3 0.3 0.3 Polyoxyethylene Isodecyl 6 0.33 0.33 0.33 0.33 Fragrance 0.007 0.007 0.007 0.007

Example IIIA

[0100]

TABLE-US-00012 TABLE 5.1 Quantatative Method For Antimicrobial Efficacy By Absorbtion Method Iso20743: 2013 For Staphylococcus Aureus Attc 6538 For Laundry Complete Table 5 Formula Trial 15 DIFFERENCE OF DIFFERENCE OF EXTREMES EXTREMES ANTI- CONCEN- FOR 3 CONTROL FOR 3 TEST GROWTH GROWTH MICROBIAL TRATION SPECIEMENS SPECIEMENS VALUE OF F VALUE OF G ACTIVITY OF (LOG) (LOG) (F = LOGC.sub.t − (G = LOGT.sub.t − VALUE HRS INOCULUM (CONDITION <1 (CONDITION <2 LOGC.sub.0) LOGT.sub.0) (A = F − G) 1.4 × 10.sup.5 3.4 −3.3 5.7 LGC.sub.t: +7.7 LGT.sub.t: +2.0 LGC.sub.0: +4.3 LGT.sub.0: +4.3  0 HR 0.2 0.0 24 HR 0.1 0.0

TABLE-US-00013 TABLE 5.2 Quantatative Method For Antimicrobial Efficacy By Absorbtion Method Iso 20743: 2013 For Klebsiella Pneumoniae ATCC 4352 For Laundry Complete Table 5 Formula Trial 15 DIFFERENCE OF DIFFERENCE OF EXTREMES EXTREMES ANTI- CONCEN- FOR 3 CONTROL FOR 3 CONTROL GROWTH GROWTH MICROBIAL TRATION SPECIEMENS SPECIEMENS VALUE OF F VALUE OF G ACTIVITY OF (LOG) (LOG) (F = LOGC.sub.t − (G = LOGT.sub.t − VALUE HRS INOCULUM (CONDITION <1 (CONDITION <2 LOGC.sub.0) LOGT.sub.0) (A = F − G) 3.1 × 10.sup.5 +3.0 −2.7 5.7 LGC.sub.t: +7.7 LGT.sub.t: +2.0 LGC.sub.0: +4.7 LGT.sub.0: +4.7  0 HR 0.5 0.0 24 HR 0.0 0.0

Example IV

[0101] With the SARS CoV2 pandemic affecting the world, a series of experiments at the University of Alabama Huntsville Ga. under Dr Debra Moriarity were conducted with a SARS CoV-2 CDC surrogate, namely a Murine Hepatitis virus, MHV-S. MHV-S was grown in a mouse liver cell line, NCTC clone 1469. Isolated virus was incubated with a dilution of the Laundry Complete based on its use instructions, at room temperature for 15 minutes. The samples were then neutralized and passed over a dextran bead column to remove potential cytotoxic substances. Column eluates were used to inoculate NCTC done 1469 cells in a 96 well plate. After 7 days the wells were examined for cytopathological effects (CPE) in the cells and scored accordingly. The protocol followed DIN EN-14476 European Standard for Quantitative Suspension Test of Chemical Disinfectants and Antiseptics for Virucidal Activity, December 2015.

[0102] Cell culture: NCTC Clone 1469 (ATCC® CCL-9.1™) was maintained in DMEM with 4500 g/l glucose plus L-gln and 1.5 g/l sodium bicarbonate, pH 7.3, supplemented with 10% Donor Horse Serum (Biotechne, Minneapolis, Minn.) in a humidified incubator at 37° C. and 5% CO.sub.2. Cells were passaged by scraping cells from the flask surface, centrifuging and resuspending in new growth media. 5×10.sup.4 cells/well were plated in DMEM+10% horse serum in a 96 well plate 24 hours before the assay and incubated as above.

[0103] Virus preparation: MHV-S (ATCC VR-766™) was used to inoculate NCTC Clone 1469 cells at a moi of about 1.0 following published procedures (Leibowitz et al., 2011). Isolated virus was stored at ˜80° C. in 1.0 ml aliquots. Virus titer was determined using the endpoint dilution procedure to obtain the TCID.sub.5o on the NCTC Clone 0.1469 cells. Suspension Test Laundry Complete, Lot 05062020-LC-A, was diluted at a ratio of 4.69 mi to 250 ml with ultrapure water. All tests contained 0.03% bovine serum albumin (BSA) as a clean interfering substance.

[0104] Sterile tubes were set up as follows:

[0105] Tube A: Virus Titer: 0.1 ml stock virus, 0.1 ml 0.3% BSA, 0.8 ml PBS (phosphate buffered saline)

[0106] Tube B: Product Test: 0.1 ml stock vims, 0.1 ml 0.3% BSA, 0.8 ml diluted Laundry Complete

[0107] Tube C: Cytotoxicity Control: 0.1 ml DMEM+2% horse serum (DMEM-2), 0.1 ml 0.3% BSA, 0.8 ml diluted Laundry Complete

[0108] Tube D: Virus killing control: 0.1 ml stock virus, 0.1 ml 0.3% BSA, 0.2 ml 1.4% Formaldehyde

[0109] All tubes were incubated for 15 minutes at 21.8° C., 52% relative humidity. At the end of that incubation 1.0 ml of Dey/Engly Broth (DIE broth) neutralizer was added to tubes A, B and C and 0.4 ml of DIE broth was added to tube D. All tubes were place on ice. A 500 μl sample from each tube was placed on a 4.0 ml column of Sephadex LH-20 and centrifuged at 100×g at 4° C. for 10 min. The eluant was collected and serial 10-fold dilutions were made in ice-cold DMEM.

[0110] Media was removed from cells in the 96-well plate and 100 μl samples from each test condition were added to quadruplicate wells. The plates were incubated at 37° C. and 5% CO.sub.2 for 7 days, at which time they were examined for CPE.

TABLE-US-00014 TABLE 6.1 Results - Test done on Jul. 3, 2020; plate scored on Jul. 10, 2020 Dilution (Log 10) Virus control Test Product Cytotoxicity Cell Control −2 ++++ CT ++++ 0000 −3 ++++ 0++0 0000 NA −4 ++++ 0000 0000 NA −5 ++++ 0000 0000 NA −6 ++++ 0000 0000 NA −7 +00+ 0000 0000 NA Log10TCID.sub.50 −7.0 −3.0 −2.5 NA Log 10 Reduction 4.0 NA % kill >99.99% NA + CPE (cytopathic/cytotoxic effect) present 0 CPE (cytopathic/cytotoxic effect) not detected N/A Not applicable CT Cytotoxicity
A neutralizer cytotoxicity control, run separately, showed no CPE at any dilutions of the DIE broth.
Formaldehyde killed all cells to the 10.sup.4 dilution.
The results showed significant kill.
The virus TCID.sub.50 (log 10) was determined to be 7.0
The virus TCID.sub.50 (log 10) after treatment with Laundry Complete was 3.0.
The log difference between them was 4.0 indicating 99.99% of the vims was killed.

Example V

[0111] Dimethyloctadecyl (3-trimethoxysilyl) propyl ammonium chloride is known to covalently bind to textile fabrics and will maintain a presence for up to 25 industrial washes. Dimethyloctadecyl (3-trimethoxysilyl) propyl ammonium chloride is also known to be bacteriastatic and fungistatic but not bactericidal, viricidal nor fungicidal. The Laundry Complete formulation containing ammonium chloride quats, and polyhexamethylene biguanide hcl form a polymeric nano composite matrix which imparts antiviral traits which are persistent. Continuing with the SARS CoV2, the second of a series of experiments at the University of Alabama Huntsville Ga. under Dr Debra Moriarity were conducted with a SARS CoV-2 CDC surrogate, namely a Murine Hepatitis virus, MHV-S. MHV-S was grown in a mouse liver cell line, NCTC clone 1469

[0112] Isolated virus was incubated with a piece of fabric treated with My Shield Laundry Complete (Treated on Dec. 16, 2019 and kept in a sterile container since then). Controls were untreated fabric pieces of the same size. After incubation with the virus for 15, 30 or 60 minutes the samples were neutralized with D/E broth, vortexed to remove the virus from the fabric and passed over a Sephadex LH-20 column to remove potential cytotoxic substances. Serial dilutions of the column eluates were used to inoculate NCTC clone 1469 cells in a 96 well plate to determine the TCID.sub.50 for the virus at each time. After 14 days the wells were examined for cytopathological effects (CPE) in the cells and scored accordingly.

TABLE-US-00015 TABLE 6.2 TREATED TEXTILE WITH LAUNDRY COMPLETE AT DAY 200 AND RESULTS AS ANTIVIRAL BIOCIDE Dilution Virus, no 15 minute** 30 minute** 60 minute** (−Log.sub.10) cloth Control Treated Control Treated Control treated Cytotoxicity 2 ++++ ++++ ++++ ++++ ++++ ++++ ++++ ++++ ++++ ++++ ++++ ++++ ++++ ++++ ++++ ++++ 3 ++++ ++++ ++++ ++++ 0+++ ++++ ++++ 0000 ++++ ++++ ++++ ++++ ++++ ++++ ++++ 0000 4 ++++ ++++ ++00 +0++ 0000 ++++ +00+ 0000 ++++ ++0+ 0000 +0++ +++0 ++++ 0+0+ 0000 5 000+ 0+00 00+0 ++00 0000 +0++ 000+ 0000 00+0 000+ 0000 ++0+ 00+0 ++0+ 0000 0000 6 0000 0000 0000 0000 0000 0++0 0000 ND 0000 0000 00+0 0000 0000 +0+0 0000 7 0000 0+00 0000 0000 0000 0+00 0000 ND 0000 0000 0000 00+0 0000 0000 0000 −Log.sub.10 4.67 4.44 3.67 5.20 3.75 6.00 4.00 2.50 TCID.sub.50/ml Log ≥0.77 ≥1.45 ≥2.0 NA difference* % kill ≥83.2% ≥96.5 ≥99% + = CPE 0 = live cells still visible ND = not determined *between control and treated fabric coupon for each time point **time virus was exposed to the fabric coupon

Results

[0113] The virus TCID.sub.50 (log 10) was determined to be 4.67
Cytotoxicity was only observed at 10.sup.−2 dilution.
A neutralizer cytotoxicity control, run separately, showed no CPE at any dilutions of the D/E broth.
Formaldehyde killed all cells to the 10.sup.−2 dilution.
Most significantly, the persistence of the virucidal activity of the My Shield Laundry Complete was evident even after more than 200 days with at least 99% kill of virus after only an hour of contact with the fabric, and nearly 97% kill after only 30 min.

Example VI

[0114] In a further experiment with actual wild strain SARS CoV-2 done in a BSL3 laboratory, a cotton textile product treated with Laundry Complete on Feb. 6, 2021. The sample was inoculated and tested 53 days later on Apr. 5-9, 2021.

[0115] A test suspension of the SARS-CoV-2 virus was inoculated onto My-Shield Laundry Complete coated fabric #06302020-LC-A (100% cotton) and its respective control fabric (100% cotton) surface.

[0116] The surfaces loaded with virus inoculum were maintained at specified temperature (25° C.±1° C.) for a contact period of 2 hours. At the end of the contact time remaining infectious virus particles were recovered individually from onto My-shield Laundry Complete #06302020-LC-A coated fabric specimen and its respective control fabric specimen by washing the surfaces followed by vortex and agitation in neutralizing medium. Quantification of recovered surviving organisms (infectious virus particles) was done by plaque assay. As prescribed in guideline assay was performed in triplicate using 3 test specimen for each step.

TABLE-US-00016 TABLE 7.1 VIRUS DATA Realm Riboviria Order Nidovirales Family Coronaviridae Genus Betacoronavirus Species COVID-19 NCBI Accession number MT416726[MB1] for virus isolate

TABLE-US-00017 TABLE 7.2 TEST OUTLINE Guideline referred ISO 18184 (Textiles: Determination of Antiviral activity of textile products) Details of the specimen My-shield Laundry Complete coated fabric Lot #06302020-LC-A (100% cotton) and control fabric (100% cotton) of size 20 mm X 20 mm, 0.4 g Date of coating of test sample 6.sup.th Feb. 2021 as provided by Zeta Tech Assay performed on 53 days after treatment My-shield Laundry Complete onto the fabric specimen Specimen storage condition Ambient Virus used for testing COVID 19 (SARS-CoV-2) P6 Host Cell line used for testing Vero cell line Pl56 Volume of test inoculum used 200 μI (0.7 × 10.sup.5 PFU/ml) Test Concentrations RTU (150 ml per 4.5 kg fabric in standard wash) refer CoAProduct Safety Labs # 53706 Test Temperature 25° C. ± 1° C. Temperature of 37° C. incubation in plaque assay Contact time 2 hours Stability and appearance of the 200 μl of the virus specimen was specimen during the test absorbed over a period of 2 hours in test and control specimen upper layer only. Neutralizer used Ice cold 2% Minimal Essential media

[0117] My-Shield Laundry Complete Lot #06302020-LC-A coated fabric has exhibited antiviral activity value Mv of 2,643 against SARS-CoV2 virus after 2 hours of contact time when tested after 53 days applying coating onto the fabric (100% cotton) specimen.

[0118] The antiviral efficacy of My-shield Laundry Complete coated fabric Lot #06302020-LC-A tested after 53 days of coating onto the specimen (100% cotton fabric) has a GOOD EFFECT’ as per the antiviral performance standard against SARS-CoV2.

[0119] The results indicates that My-shield Laundry Complete treated fabric specimen Lot #06302020-LC-A exhibited virucidal activity against SARS-CoV2 virus. No live infectious SARS-CoV-2 virus particles were retained on My-shield Laundry Complete treated fabric specimen Lot #06 A panel than that of respective untreated control specimen after contact time of 2 hours.

TABLE-US-00018 TABLE 7.3 DATA SUMMARY V.sub.P Number of plaques recovered (PFU/ M Mν Specimen 1 Specimen 2 Specimen 3 z vial) value value Untreated A 25 21 22 25 13 9 19.166 Vb 19166 0.36B 2.643 Treated A# ND ND ND ND ND ND  1#    Ve 1000 Control A 49 48 28 25 59 55 44    Va 44000 A Dilution Factor: 10; #As the sample is cytotoxic at 10.sup.−1, the plaque were not determined. Since no plaques were observed in 10.sup.−2 dilution, following ISO18184 guideline the number of plaques is recorded as ‘1’ Logarithm reduction value of infective titre of control specimen should be ≤1.0.

[0120] The contents of all references cited in the instant specifications and all cited references in each of those references are incorporated in their entirety by reference herein as if those references were each individually denoted in the text.

[0121] While the many embodiments of the invention have been disclosed above and include presently preferred embodiments, many other embodiments and variations are possible within the scope of the present disclosure and in the appended claims that follow. Accordingly, the details of the preferred embodiments and examples provided are not to be construed as limiting. It is to be understood that the terms used herein are merely descriptive rather than limiting and that various changes, numerous equivalents may be made without departing from the spirit or scope of the claimed invention.