Assembled bioreactor chamber suitable for perfusion culture

Abstract

Provided is an assembled bioreactor chamber comprising an upper end portion, at least one intermediate portion and a lower end portion for forming a chamber of the reactor chamber. The upper end portion and the lower end portion are respectively provided with an interface connected with a culture medium line, and the upper end portion and the intermediate portion, and the intermediate portion and the lower end portion are detachably connected in a sealed manner through a connecting mechanism; when the number of the intermediate portions is ≥2, the adjacent intermediate portions are detachably connected in a sealed manner through a connecting mechanism; and the chamber also comprises a cell carrier for cell attachment growth.

Claims

1. An assembled bioreactor chamber suitable for perfusion culture, said bioreactor chamber comprising an upper end portion, one or more intermediate portions and a lower end portion which are successively connected, and said upper end portion, said one or more intermediate portions and said lower end portion forming a chamber of the assembled bioreactor chamber, wherein said upper end portion is provided with an upper end portion interface for connecting with a culture medium line, said lower end portion is provided with a lower end portion interface for connecting with a culture medium line, and the upper end portion and the one or more intermediate portions are detachably connected in a sealed manner, the one or more intermediate portions and the lower end portion are detachably connected in a sealed manner, and when the number of the one or more intermediate portions is ≥2, adjacent intermediate portions are detachably connected with each other in a sealed manner; wherein at least one barrier sheet is integrated onto the one or more intermediate portions for changing the flow direction of a culture medium, wherein said assembled bioreactor chamber is a fixed bed bioreactor chamber or a fluidized bed bioreactor chamber.

2. The assembled bioreactor chamber according to claim 1, wherein said assembled bioreactor chamber comprises bolts and nuts for integrally securing said upper end portion, said one or more intermediate portions and said lower end portion.

3. The assembled bioreactor chamber according to claim 2, wherein said assembled bioreactor chamber further comprises plug-in slots and plugs which are matched to each other, said plug-in slots and said plugs are provided on connecting end faces of said upper end portion, said one or more intermediate portions and said lower end portion.

4. The assembled bioreactor chamber according to claim 3, wherein said plug-in slots extend inwardly and perpendicularly to the end faces and show an L-shape, and said plugs extend outwardly and perpendicularly to the end faces and show an L-shape.

5. The assembled bioreactor chamber according to claim 3, wherein the number of the one or more intermediate portions of said assembled bioreactor chamber is ≥2, and said assembled bioreactor chamber comprises two kinds of intermediate portions having different internal space volumes, with a ratio of the internal space volume of one kind of intermediate portion to the internal space volume of the other kind of intermediate portion being from 1:2 to 1:5.

6. The assembled bioreactor chamber according to claim 3, wherein said assembled bioreactor chamber is a fixed bed bioreactor chamber, said one or more intermediate portions are provided with a basket for supporting cell growth, and said basket is filled with a cell carrier for cell attachment growth.

7. The assembled bioreactor chamber according to claim 2, wherein the number of the one or more intermediate portions of said assembled bioreactor chamber is ≥2, and said assembled bioreactor chamber comprises two kinds of intermediate portions having different internal space volumes, with a ratio of the internal space volume of one kind of intermediate portion to the internal space volume of the other kind of intermediate portion being from 1:2 to 1:5.

8. The assembled bioreactor chamber according to claim 2, wherein said assembled bioreactor chamber is a fixed bed bioreactor chamber, said one or more intermediate portions are provided with a basket for supporting cell growth, and said basket is filled with a cell carrier for cell attachment growth.

9. The assembled bioreactor chamber according to claim 2, wherein, said at least one barrier sheet is provided within the chamber and arranged in such a staggered manner that the culture medium forms a zigzag flow in the chamber.

10. The assembled bioreactor chamber according to claim 1, wherein the number of the one or more intermediate portions of said assembled bioreactor chamber is ≥2, and said assembled bioreactor chamber comprises two kinds of intermediate portions having different internal space volumes, with a ratio of the internal space volume of one kind of intermediate portion to the internal space volume of the other kind of intermediate portion being from 1:2 to 1:5.

11. The assembled bioreactor chamber according to claim 10, wherein the ratio of the number of the intermediate portions having a larger internal space volume to the number of the intermediate portions having a smaller internal space volume is from 1:1 to 1:5.

12. The assembled bioreactor chamber according to claim 10, wherein said assembled bioreactor chamber is a fixed bed bioreactor chamber, said one or more intermediate portions are provided with a basket for supporting cell growth, and said basket is filled with a cell carrier for cell attachment growth.

13. The assembled bioreactor chamber according to claim 1, wherein said assembled bioreactor chamber is a fixed bed bioreactor chamber, said one or more intermediate portions are provided with a basket for supporting cell growth, and said basket is filled with a cell carrier for cell attachment growth.

14. The assembled bioreactor chamber according to claim 13, wherein the cell carrier is a polyester fiber carrier, a polystyrene fiber carrier, a porous polymer material carrier or a porous ceramic material carrier.

15. The assembled bioreactor chamber according to claim 1, wherein, said at least one barrier sheet is provided within the chamber and arranged in such a staggered manner that the culture medium forms a zigzag flow in the chamber.

16. The assembled bioreactor chamber according to claim 15, wherein the length of said at least one barrier sheet is from 6/10 to 9/10 times a cross-sectional dimension of the chamber.

17. The assembled bioreactor chamber according to claim 15, wherein the length of said at least one barrier sheet is 4/5 times a cross-sectional dimension of the chamber.

18. The assembled bioreactor chamber according to claim 1, wherein said assembled bioreactor chamber further comprises sealing rings for connecting said upper end portion, said one or more intermediate portions and said lower end portion in a sealed manner.

19. The assembled bioreactor chamber according to claim 1, wherein the number of the at least one barrier sheet is equal to or less than the number of the one or more intermediate portions.

20. An assembled bioreactor chamber suitable for perfusion culture, said bioreactor chamber comprising an upper end portion, one or more intermediate portions and a lower end portion which are successively connected, and said upper end portion, said one or more intermediate portions and said lower end portion forming a chamber of the assembled bioreactor chamber, wherein said upper end portion is provided with an upper end portion interface for connecting with a culture medium line, said lower end portion is provided with a lower end portion interface for connecting with a culture medium line, and the upper end portion and the one or more intermediate portions are detachably connected in a sealed manner, the one or more intermediate portions and the lower end portion are detachably connected in a sealed manner, and when the number of the one or more intermediate portions is ≥2, adjacent intermediate portions are detachably connected with each other in a sealed manner; wherein at least one barrier sheet is integrated onto the one or more intermediate portions for changing the flow direction of a culture medium, wherein the number of the at least one barrier sheet is equal to or less than the number of the one or more intermediate portions.

Description

BRIEF DESCRIPTION OF FIGURES

(1) Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings, in which:

(2) FIG. 1 is a schematic view of an upper end portion of an assembled bioreactor chamber in accordance with one embodiment of the present invention.

(3) FIG. 2 is a schematic exploded view of an assembled bioreactor chamber in accordance with the present invention, including an upper end portion and an intermediate portion.

(4) FIG. 3 is a schematic view of an assembled bioreactor chamber in accordance with one embodiment of the present invention.

(5) FIG. 4 is a schematic exploded view of an assembled bioreactor chamber in accordance with one embodiment of the present invention.

(6) FIG. 5 is a schematic view of an assembled bioreactor chamber in accordance with one embodiment of the present invention after being assembled.

(7) FIG. 6 is a schematic view of the flow state of a culture medium within an assembled bioreactor chamber in accordance with the present invention.

(8) FIG. 7 is a schematic view of another flow state of a culture medium within an assembled bioreactor chamber in accordance with the present invention.

(9) FIG. 8 is a schematic view of an assembled bioreactor chamber in accordance with one embodiment of the present invention after being assembled.

BEST MODE FOR CARRYING OUT THE INVENTION

(10) The present invention is described in detail with reference to specific embodiments. The examples are given merely to illustrate the present invention and are not intended to limit the protection scope of the present invention.

Example 1

(11) Referring to FIG. 1 to FIG. 5, these figures show an assembled bioreactor chamber, which comprises an upper end portion 1, a plurality of intermediate portions 2, a lower end portion 3 and sealing rings 6.

(12) The upper end portion 1 is provided with an upper end portion interface 11 for connecting with a culture medium line, and the lower end portion 3 is provided with a lower end portion interface 31 for connecting with a culture medium line. The intermediate portion 2 is provided with a basket for supporting cell growth (not shown), and said basket is filled with a polyester fiber carrier.

(13) The upper end portion 1 and the intermediate portion 2, the intermediate portion 2 and the lower end portion 3, and the adjacent intermediate portions 2 can be detachably connected in a sealed manner through a connecting mechanism. Said connecting mechanism comprises L-shaped plug-in slots 7 and L-shaped plugs 8 which are matched to each other, and bolts 4 and nuts 5 which are matched to each other, and the L-shaped plug-in slots 7 and the L-shaped plugs 8 are provided on the connecting end faces of the upper end portion 1, the intermediate portion 2 and the lower end portion 3. Two plug-in slots 7 and two plugs 8 are respectively provided on each connecting end face, and the plug-in slots and the plugs are arranged at intervals. In addition, the upper end portion 1, the intermediate portion 2 and the lower end portion 3 are provided with holes for mounting the bolts 4.

(14) During assembly, an upper end portion 1, a plurality of intermediate portions 2 and a lower end portion 3 are arranged successively from top to bottom, and a sealing ring 6 is placed between the portions respectively. An intermediate portion 2 is pushed towards the lower end portion 3 in the axial direction of the assembled bioreactor chamber, so that the plugs 8 is inserted into the plug-in slots 7, and then the intermediate portion 2 is rotated. Intermediate portion 2 and the upper end portion 1 are successively assembled in accordance with this method, and then the portions are secured with the bolts 4 and the nuts 5. The upper end portion 1, the plurality of intermediate portions 2 and the lower end portion 3 thereby form a chamber of the assembled bioreactor chamber.

(15) As shown in FIG. 6, in Example 1, the culture medium can form a through flow along the direction indicated by the arrow.

Example 2

(16) The assembled bioreactor chamber of this Example is substantially the same as that of Example 1, except that the intermediate portion 2 is provided with a barrier sheet 21, and adjacent barrier sheets are arranged in a staggered manner, wherein the length of the barrier sheet 21 is 4/5 times the cross-sectional dimension of the chamber. As indicated by the arrows in FIG. 7, the culture medium can form a zigzag flow in the chamber.

Example 3

(17) The assembled bioreactor chamber of this Example is substantially the same as that of Example 1, except that said connecting mechanism comprises bolts 4 and nuts 5, and the upper end portion 1, the intermediate portion 2 and the lower end portion 3 are provided with holes for mounting the bolts 4.

(18) Referring to FIG. 8, during assembly, an upper end portion 1, a plurality of intermediate portions 2 and a lower end portion 3 are arranged successively from top to bottom, and sealing rings 6 are placed between the portions respectively, then secured with the bolts 4 and the nuts 5. The upper end portion 1, the plurality of intermediate portions 2 and the lower end portion 3 thereby form a chamber of the assembled bioreactor chamber.