Use

Abstract

The present invention relates to the use of erythrulose as antimicrobial agent as well as to composition comprising said agent.

Claims

1. A method of inhibiting microbial growth of microbes selected from the group consisting of Propionibacterium acnes (P. acnes), Staphylococcus epidermis (S. epidermis), Cornyebacterium xerosis (C. xerosis), Aspergillus brasiliensis (A. brasiliensis) and mixtures thereof in a product composition, wherein the method comprises incorporating into the product composition in need of microbial inhibition an antimicrobial growth inhibiting amount of erythrulose.

2. The method according to claim 1, wherein the product composition is selected from the group consisting of cosmetic compositions, household product compositions, plastic product compositions, paper product compositions and paint product compositions.

3. The method according to claim 2, wherein the method improves preservation of the product composition against microbial decay and breakdown as compared to a product composition not containing the erythrulose.

4. The method according to claim 1, wherein the erythrulose is incorporated into the product composition as a deodorant active compound.

5. The method according to claim 1, wherein the erythrulose is incorporated into a cosmetic product composition as an anti-acne compound.

6. The method according to claim 1, wherein the method prevents the microbial decay and breakdown caused by A. brasiliensis of the product composition, and wherein the method comprises adding to the product composition an effective antimicrobial decay and breakdown preventing amount in a range of 0.005 to 0.75 wt. %, based on the total weight of the product composition, of the erythrulose.

7. The method according to claim 6, wherein the effective antimicrobial decay and breakdown preventing amount of the erythrulose is 0.01 to 0.6 wt. %, based on the total weight of the product composition.

8. The method according to claim 6, wherein the product composition further comprises water and at least one agent selected from the group consisting of surfactants, emulsifiers, thickeners and oils.

9. The method according to claim 6, wherein the product composition is a cosmetic composition in a form of a shampoo preparation, a hair conditioner, an oil-in-water (O/W) emulsion, a water-in-oil (W/O) emulsion or a gel.

10. The method according to claim 6, wherein the effective antimicrobial decay and breakdown preventing amount of the erythrulose is 0.025 to 0.5 wt. %, based on the total weight of the product composition.

11. The method according to claim 6, wherein the effective antimicrobial decay and breakdown preventing amount of the erythrulose is 0.04 to 0.05 wt. %, based on the total weight of the product composition.

Description

EXAMPLE 1: ANTIMICROBIAL EFFICACY

(1) The antimicrobial efficacy is assessed in analogy to the regulatory challenge test method (NF EN ISO11930). Thus, a solution of Erythrulose from DSM Nutritional products in two different concentrations was prepared under sterile conditions. For this the Erythrulose was solubilized in physiological serum with 0.85 wt.-% NaCl. Phenoxyethanol was solubilized in a physiological serum with 0.85 wt.-% NaCl further comprising 5 wt.-% ethanol to ensure solubility. The solutions were deposed in 96-deep well plates (1.6 ml/well). The wells were contaminated with the respective bacterial or the fungal strains at 1.9*10.sup.5 to 8.1*10.sup.5 cfu/ml for the bacteria and 3.3*10.sup.3 to 3.8*10.sup.4 cfu/ml for the fungi to obtain the initial contamination as outlined in table 1 and 2. After contamination each well was thoroughly mixed to ensure a homogeneous distribution of the microorganism. Then each plate was incubated at 22° C. for 24 h. The counting of the (remaining) population was carried out 24 h after contamination.

(2) TABLE-US-00001 TABLE 1 Erythrulose, 0.4% active Erythrulose (0.4 wt.-%) Phenoxyethanol (0.5 wt.-%) colony counts [cfu/ml] colony counts [cfu/ml] Bacteria/fungi 0 h 24 h 0 h 24 h P. Acnes 500000 55000 500000 260000 (acne, gram+) S. epidermidis 500000 0 500000 3300 (deo, gram+) C. xerosis 810000 0 810000 0 (deo, gram+) A. brasiliensis 38000 330 38000 78 (preserv., mold) C. albicans 19000 78 19000 1000 (preserv., yeast) P. aeruginosa 810000 0 810000 280000 (preserv., gram−) E. coli 440000 280000 440000 530000 (preserv., gram−) S. aureus 190000 0 190000 78000 (preserv., gram+)

(3) TABLE-US-00002 TABLE 2 Erythrulose, 0.08% active t0 24 h Propionibacterium acnes 310000 3300 (acne, gram+) Staphylococcus epidermidis 560000 0 (deo, gram+) Corynebacterium xerosis 250000 55 (deo, gram+) Aspergillus brasiliensis 10000 10 (preserv., mold) Pseudomonas aeruginosa 450000 530000 (preserv., gram−) Escherichia coli 250000 530000 (preserv., gram−)

(4) As can be retrieved from table 1 erythrulose exhibits an equal or even better activity against microbes as the preservative phenoxyethanol, a well-known preservative in cosmetics, even at lower concentrations (0.4 wt.-% vs 0.5 wt. %).

(5) Furthermore, the results in table 2 illustrate that Erythrulose is particularly and selectively active against microbial cells responsible for the odor formation from sweat e.g. in the axilla, i.e. S. epidermidis and C. xerosis even at very low concentrations, which makes it particularly suitable for deodorant applications while at this concentration level it is not active anymore against other microbes such as P. aeruginosa and E. coli.

(6) The results in table 2 furthermore illustrate that erythrulose at very low concentration still excerpts a good antimicrobial activity against P. acnes, making it suitable for a mild anti-acne treatment.

(7) Furthermore, it also suitable at such low concentration levels to protect products from microbial decay caused by A. brasiliensis.

EXAMPLE 2: O/W FOUNDATION

(8) TABLE-US-00003 Ingredients INCI wt. % Deionised Water Aqua Ad 100 Glycerin Glycerin 2.00 Triethanolamine Triethanolamine 0.80 Paratexin M Methylparaben EP 0.20 Keltrol Xanthan Gum 0.30 Erythrulose Erythrulose 0.10 Titanium dioxide C.I. 77891 4.57 SunCROMA yellow iron oxide C.I. 77492 0.30 SunCROMA red iron oxide C.I. 77491 0.13 SunCROMA black iron oxide C.I. 77499 0.20 DC 556 Phenyl Trimethicone 3.60 Stearic Acid Stearic Acid 1.4 Cetyl Alcohol Cetyl Alcohol 3.0 Paratexin P Propylparaben EP 0.1

EXAMPLE 3: ALCOHOL FREE FACIAL TONIC

(9) TABLE-US-00004 Ingredients INCI wt. % Polysorbate-20 Polysorbate-20 2.00 ALPAFLOR CALENDULA Calendula Officinalis 0.80 AO Extract, Glycerin, Water ALPAFLOR BUDDLEJA Buddleja Davidii 0.80 AO Extract, Glycerin, Water Arlasilk Phospholipd Sodium Coco PG-Dimonium 0.50 CDM Chloride Phosphate Fragrance Parfum 0.10 Deionised Water Aqua Ad 100 Citric Acid Citric Acid 0.01 Erythrulose Erythrulose 0.75 Paratexin FRP Ethylparaben, Butylparaben, 0.10 Propylparaben, Isobutylparaben

EXAMPLE 4: W/O CREAM

(10) TABLE-US-00005 Ingredients INCI wt. % Cremophor WO-7 PEG-7 Hydrogenated Castor Oil 2.50 Elfacos ST-9 PEG-45/Dodecyl Glycol Copolymer 2.00 Cirebelle 303 Synthetic Wax 5.00 Cirebelle 109L Synthetic Wax 7.20 Miglyol 818 Caprylic/Capric/Linoleic Triglyceride 5.00 Eutanol G Octyldodecanol 7.50 Cetiol OE Dicaprylyl Ether 9.20 Deionised Water Aqua Ad 100 Glycerin Glycerin 5.00 Propylene Glycol Propylene Glycol 2.00 Euxyl PE 9010 Phenoxyethanol and Ethylhexylglycerin 0.80 Erythrulose Erythrulose 0.1 

EXAMPLE 5: SOOTHING GEL

(11) TABLE-US-00006 Ingredients INCI wt. % Deionised Water Aqua Ad 100 Keltrol CG RD Xanthan Gum 0.50 Sodium Benzoate Sodium Benzoate 0.20 Potassium Sorbate Potassium Sorbate 0.25 ALPAFLOR Glycerin, Aqua, 3.00 MARRABIUM Marrubium Vulgare, AO Sodium Benzoate, Potassium Sorbate Erythrulose Erythrulose 0.40

EXAMPLE 6: O/W LOTION

(12) TABLE-US-00007 Ingredients INCI wt. % Deionised Water Aqua Ad 100 Menthol Menthol 0.10 Keltrol CG SFT Xanthan Gum 1.25 Ceralution ES Ceteareth-25, Di Sodium Ethylene 2.00 Dicocamide PEG-15 Disulfate Isofol 20 Octyldodecanol 5.00 Paratexin EC5 Benzoic Acid Benzyl Alcohol, 1.00 Dehydroacetic Acid, Sorbic Acid Erythrulose Erythrulose 0.25

EXAMPLE 7: FACIAL CLEANSING GEL

(13) TABLE-US-00008 Ingredients INCI wt. % Deionised Water Aqua Ad 100 Carbopol AQUA Acrylates Copolymer 7.50 SF-1 Polymer Texapon NSO-BZ Sodium Laureth Sulfate 41.00  Miranol Ultra C 32 Sodium Cocoamphoacetate 5.00 Hostapon CLG Sodium Lauroyl Glutamate 4.50 Jaguar C162 Hydroxypropyl Guar Hydroxypro- 1.00 pyltrimonium Chloride Erythrulose Erythrulose 0.50 Euxyl K 300 Phenoxyethanol & Methylparaben & 0.80 Propylparaben & Ethylparaben & Butylparaben & Isobutylparaben ALPAFLOR MALVA Glycerin, Aqua, Malva Sylvestris 2.00 AO (Mallow) Flower Extract, Potassium Sorbate, Sodium Benzoate Parfum Limette Fragrance q.s. FD&C Yellow 5 CI 19140 0.50 Frescolat Plus Menthyl Lactate, Menthol 0.20 Dehyton AB-30 Coco Betaine 2.00 Rewoderm LI S 80 PEG-200 Hydrogenated Glyceryl 1.00 Palmate & PEG-7 Glyceryl Cocoate Citric Acid Citric Acid q.s.

EXAMPLE 8: LEAVE-ON HAIR AND SCALP CONDITIONER

(14) TABLE-US-00009 Ingredients INCI wt. % Deionised Water Aqua Ad 100 Ethanol DEB 96 Alcohol denat. 30.00 PVP/VA Copolymer PVP/VA Copolymer 2.50 Euxyl K-300 Phenoxyethanol, Methylparaben, 0.80 Butylparaben, Ethylparaben, Propylparaben, Isobutylparaben Protachem HCO-40 PEG-40 Hydrogenated Castor Oil 0.50 Fragrance ADAM Parfum 0.10 Triethanolamine 99% Triethanolamine 0.01 FD & C Yellow No 5 CI 19140, Aqua 0.10 (0.5% Solution) FD & C Blue No 1 CI 42090, Aqua 0.10 (0.5% Solution) Erythrulose Erythrulose 0.05

EXAMPLE 9: SHAMPOO

(15) TABLE-US-00010 INCI Nomenclature wt. % Aqua (water) Ad 100 Ammonium laureth sulfate 10.00  Ammonium lauryl sulfate 5.00 Glycol distearate 1.00 Dimethicone 1.00 Cetyl alcohol 0.50 Cocamide MEA 3.00 Erythrulose 0.30 ZPT 0.50 Guar hydroxypropyltrimonium chloride 0.20 Hydrogenated polydecene 1.00 Polyquaternium 10 0.30 PEG 7m 0.50 Trimethylpropane tricaprylate/tricaprate 1.00 Preservative q.s. Fragrance 0.30 E 104, E 110, E 132 0.02

EXAMPLE 10: CLEAR SHAMPOO WITH PLANT EXTRACTS

(16) TABLE-US-00011 INCI Nomenclature wt. % Aqua (water) Ad 100 Sodium laureth sulfate 10.00 Lauryl glucoside 6.00 Cocamidopropyl betaine, 2.00 Propylene glycol 2.00 Perfume oil 1.25 Sodium citrate 0.25 Sodium benzoate 0.20 Panthenol 1.00 Sodium formate 0.20 Polyquaternium-10 0.20 Hydroxypropyl guar hydroxypropyltrimonium chloride 0.05 Erythrulose 0.25 PEG-35 castor oil 1.00 Maris sal 1.25 Polysorbate 20 1.00 Tocopheryl acetate 0.20 Prunus armeniaca 0.20 Echinacea purpurea 0.05 Tocopherol 0.05 Linoleic acid 0.20 Preservative 1.00 CI77891 0.02

EXAMPLE 11: RINSE-OFF HAIR AND SCALP CONDITIONER

(17) TABLE-US-00012 INCI Nomenclature wt. % Aqua (water) Ad 100 Stearyl alcohol 2.50 Cetyl alcohol 2.50 Behentrimonium chloride 1.30 Dimethicone 2.00 Erythrulose 0.50 Fragrance 0.50 Butylene glycol 2.00 Methyl parabene 0.30