PESTICIDAL MIXTURES

Abstract

A method for controlling phytopathogenic pests on cereals, wherein the pest, their habitat, breeding grounds, their locus or the plants to be protected against pest attack, the soil or plant propagation material are treated with an effective amount of a fungicidal mixture comprising, as active components, 1) 1-[2-[[1-(4-chlorophenyl)pyrazol-3-yl]oxymethyl]-3-methyl-phenyl]-4-methyl-tetrazol-5-one as compound I, and 2) one fungicidal compound II selected from [2, 2-bis(4-fluorophenyl)-1-methyl-ethyl] 2-[(3-acetoxy-4-methoxy-pyridine-2-carbonyl)amino]propanoate (compound 11-1) and [(1S)-2,2-bis(4-fluorophenyl)-1-methyl-ethyl](2S)-2-[(3-acetoxy-4-methoxy-pyridine-2-carbonyl)amino]propanoate (compound II-2).

Claims

1. A method for controlling a phytopathogenic pest on cereals, wherein the pest, its habitat, breeding grounds, its locus or the plant to be protected against pest attack, soil or plant propagation material is treated with an effective amount of a fungicidal mixture comprising, as active components, 1) 1-[2-[[1-(4-chlorophenyl)pyrazol-3-yl]oxymethyl]-3-methyl-phenyl]-4-methyl-tetrazol-5-one (compound I), and 2) one fungicidal compound II selected from [2,2-bis(4-fluorophenyl)-1-methyl-ethyl] 2-[(3-acetoxy-4-methoxy-pyridine-2-carbonyl)amino]propanoate (compound II-1) and [(1S)-2,2-bis(4-fluorophenyl)-1-methyl-ethyl] (2S)-2-[(3-acetoxy-4-methoxy-pyridine-2-carbonyl)amino]propanoate (compound II-2).

2. The method according to claim 1 for controlling a phytopathogenic fungus, wherein the phytopathogenic fungus is Microduchium nivale, Erysiphe graminis tritici, Septoria tritici, Phaeospheria nodorum, or Pyrenophera tritici on wheat.

3. The method according to claim 1 for controlling a phytopathogenic fungus, wherein the phytopathogenic fungus is Erysiphe graminis hordei, Pyrenophera teres, Ramularia collicygni, or Rynchosporium secalis on barley.

4. The method according to claim 1 for controlling a phytopathogenic fungus, wherein the phytopathogenic fungus is Puccinia recondita (brown or leaf rust), Puccinia striiformis (stripe or yellow rust), or Puccinia graminis (stem or black rust) on wheat, barley, or rye.

5. The method as according to claim 1, wherein said fungicidal mixture is applied simultaneously either jointly or separately, or in succession.

Description

EXPERIMENTS

[0079] Microtest

[0080] The active compounds were formulated separately as a stock solution having a concentration of 10000 ppm in dimethyl sulfoxide.

[0081] 1. Activity Against Wheat Leaf Spots Caused by Leptosphaeria nodorum (LEPTNO)

[0082] The stock solutions were mixed according to the ratio, pipetted onto a micro titer plate (MTP) and diluted with water to the stated concentrations. A spore suspension of Leptosphaeria nodorum in an aqueous biomalt or yeast-bactopeptone-glycerine or DOB solution was then added. The plates were placed in a water vapor-saturated chamber at a temperature of 18° C. Using an absorption photometer, the MTPs were measured at 405 nm 7 days after the inoculation.

[0083] 2. Activity Against Microdochium nivale (MONGNI)

[0084] The stock solutions were mixed according to the ratio, pipetted onto a micro titer plate (MTP) and diluted with water to the stated concentrations. A spore suspension of Microdochium nivale in an aqueous biomalt or yeast-bactopeptone-glycerine or DOB solution was then added. The plates were placed in a water vapor-saturated chamber at a temperature of 18° C. Using an absorption photometer, the MTPs were measured at 405 nm 7 days after the inoculation.

[0085] 3. Activity Against Net Blotch Pyrenophora teres on Barley in the Microtiter Test (PYRNTE)

[0086] The stock solutions were mixed according to the ratio, pipetted onto a micro titer plate (MTP) and diluted with water to the stated concentrations. A spore suspension of a Qoi resistant isolate of Pyrenophora teres in an aqueous biomalt or yeast-bactopeptone-glycerine or DOB solution was then added. The plates were placed in a water vapor-saturated chamber at a temperature of 18° C. Using an absorption photometer, the MTPs were measured at 405 nm 7 days after the inoculation.

[0087] 4. Activity Against Rhynchosporium secalis (RHYNSE)

[0088] The stock solutions were mixed according to the ratio, pipetted onto a micro titer plate (MTP) and diluted with water to the stated concentrations. A spore suspension of Rhynchosporium secalis in an aqueous biomalt or yeast-bactopeptone-glycerine or DOB solution was then added. The plates were placed in a water vapor-saturated chamber at a temperature of 18° C. Using an absorption photometer, the MTPs were measured at 405 nm 7 days after the inoculation.

[0089] 5. Activity Against Leaf Blotch on Wheat Caused by Septoria tritici (SEPTTR)

[0090] The stock solutions were mixed according to the ratio, pipetted onto a micro titer plate (MTP) and diluted with water to the stated concentrations. A spore suspension of a Qoi resistant isolate of Septoria tritici in an aqueous biomalt or yeast-bactopeptone-glycerine or DOB solution was then added. The plates were placed in a water vapor-saturated chamber at a temperature of 18° C. Using an absorption photometer, the MTPs were measured at 405 nm 7 days after the inoculation.

[0091] The measured parameters were compared to the growth of the active compound-free control variant (100%) and the fungus-free blank value to determine the relative growth in % of the pathogens in the respective active compounds.

[0092] These percentages were converted into efficacies.

[0093] An efficacy of 0 means that the growth level of the pathogens corresponds to that of the untreated control; an efficacy of 100 means that the pathogens were not growing.

[0094] The expected efficacies of active compound mixtures were determined using Colby's formula [R. S. Colby, “Calculating synergistic and antagonistic responses of herbicide combinations”, Weeds 15, 20-22 (1967)] and compared with the observed efficacies.

TABLE-US-00002 LEPTNO Calculated efficacy Active according compound/ Concentration Observed to Colby Synergism active mixture (ppm) Mixture efficacy (%) (%) Metyltetraprol 0.016 — 49 Florylpicoxamid 0.004 — 13 Metyltetraprol 0.016 4:1 94 55 39 Florylpicoxamid 0.004

TABLE-US-00003 MONGNI Calculated efficacy Active according compound/ Concentration Observed to Colby Synergism active mixture (ppm) Mixture efficacy (%) (%) Metyltetraprol 0.016 — 32 0.004 — 1 Florylpicoxamid 0.063 — 44 0.016 — 18 0.004 — 3 Metyltetraprol 0.016 1:1 85 44 41 Florylpicoxamid 0.016 Metyltetraprol 0.016 4:1 61 34 27 Florylpicoxamid 0.004 Metyltetraprol 0.016 1:4 99 62 37 Florylpicoxamid 0.063 Metyltetraprol 0.004  1:16 95 44 51 Florylpicoxamid 0.063

TABLE-US-00004 PYRNTE Qoi restistant Calculated efficacy Active according compound/ Concentration Observed to Colby Synergism active mixture (ppm) Mixture efficacy (%) (%) Metyltetraprol 0.063 — 11 Florylpicoxamid 0.063 — 9 Metyltetraprol 0.063 1:4 39 19 20 Florylpicoxamid 0.063

TABLE-US-00005 RHYNSE Calculated efficacy Active according compound/ Concentration Observed to Colby Synergism active mixture (ppm) Mixture efficacy (%) (%) Metyltetraprol 0.016 — 14 Florylpicoxamid 0.063 — 55 Metyltetraprol 0.016 1:4 85 61 24 Florylpicoxamid 0.063

TABLE-US-00006 SEPTTR Qoi resistant Calculated efficacy Active according compound/ Concentration Observed to Colby Synergism active mixture (ppm) Mixture efficacy (%) (%) Metyltetraprol 0.063 — 1 0.016 — 0 0.004 — 1 Florylpicoxamid 0.063 — 12 0.016 — 0 Metyltetraprol 0.063 1:1 50 13 37 Florylpicoxamid 0.063 Metyltetraprol 0.063 4:1 38 1 37 Florylpicoxamid 0.016 Metyltetraprol 0.016 1:4 41 12 29 Florylpicoxamid 0.063 Metyltetraprol 0.004  1:16 40 13 27 Florylpicoxamid 0.063